Experts and Doctors on hydrogen in Bochum, North Rhine Westphalia, Germany
Locale: Bochum, North Rhine Westphalia, Germany
Stripp S, Sanganas O, Happe T, Haumann M. The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy. Biochemistry. 2009;48:5042-9 pubmed publisher
..g., to the binding of an exogenous CO at 2Fe(H) in CO-inhibited enzyme. Our XAS studies pave the way for the monitoring of atomic level structural changes at the H-cluster during H(2) catalysis...
Czech I, Stripp S, Sanganas O, Leidel N, Happe T, Haumann M. The [FeFe]-hydrogenase maturation protein HydF contains a H-cluster like [4Fe4S]-2Fe site. FEBS Lett. 2011;585:225-30 pubmed publisher
..Presumably, only the 2Fe cluster is transferred from HydF to HydA1, thereby forming the active site...
Noth J, Krawietz D, Hemschemeier A, Happe T. Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii. J Biol Chem. 2013;288:4368-77 pubmed publisher
..reinhardtii PFR1 was also able to allow H(2) evolution in reaction mixtures containing Clostridium acetobutylicum 2[4Fe-4S]-ferredoxin and [Fe-Fe]-hydrogenase HYDA...
Winkler M, Kuhlgert S, Hippler M, Happe T. Characterization of the key step for light-driven hydrogen evolution in green algae. J Biol Chem. 2009;284:36620-7 pubmed publisher
..Mapping of relevant HydA1 and PetF residues constitutes an important basis for manipulating the physiological photosynthetic electron flow in favor of light-driven H(2) production. ..
Philipps G, Krawietz D, Hemschemeier A, Happe T. A pyruvate formate lyase-deficient Chlamydomonas reinhardtii strain provides evidence for a link between fermentation and hydrogen production in green algae. Plant J. 2011;66:330-40 pubmed publisher
..This analysis shows that the PFL1 pathway has a significant impact on hydrogen metabolism in C. reinhardtii. ..
Engelbrecht V, Rodríguez Maciá P, Esselborn J, Sawyer A, Hemschemeier A, Rüdiger O, et al
. The structurally unique photosynthetic Chlorella variabilis NC64A hydrogenase does not interact with plant-type ferredoxins. Biochim Biophys Acta. 2017;1858:771-778 pubmed publisher
..In an electrochemical characterization, the enzyme exhibited features typical of bacterial [FeFe]-hydrogenases (e.g. minor anaerobic oxidative inactivation), as well as of algal enzymes (very high oxygen sensitivity). ..