Experts and Doctors on fluorescence resonance energy transfer in Bielefeld, North Rhine Westphalia, Germany
Locale: Bielefeld, North Rhine Westphalia, Germany
Topic: fluorescence resonance energy transfer
Vogelsang J, Doose S, Sauer M, Tinnefeld P. Single-molecule fluorescence resonance energy transfer in nanopipets: improving distance resolution and concentration range. Anal Chem. 2007;79:7367-75 pubmed
..In addition, our experiments emphasize the influence of photoinduced dark states on both the mean energy transfer efficiency and the width of FRET histograms. ..
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda S, Gärtner F, Ströher E, et al
. Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast. Mol Plant. 2009;2:1273-88 pubmed publisher
..In summary, the data indicate a dynamic function of plant 2-CysPrx as redox sensor, chaperone, and regulator in the chloroplast with diverse functions beyond its role as thiol peroxidase. ..
Seidel T, Kluge C, Hanitzsch M, Ross J, Sauer M, Dietz K, et al
. Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. J Biotechnol. 2004;112:165-75 pubmed
..Excitation of CFP as donor fluorophore caused increased emission of YFP-fluorescence in protoplasts due to FRET. Our results give strong evidence for physical interaction of subunits c and a in living plant cells. ..
Seidel T, Schnitzer D, Golldack D, Sauer M, Dietz K. Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis. BMC Cell Biol. 2008;9:28 pubmed publisher
..However, neither VHA-c" isoform 1 nor any of the two VHA-e isoforms were identified at the tonoplast. This suggest a function in endomembrane specific VHA-assembly or targeting rather than proton transport. ..
Yang O, Popova O, Süthoff U, Lüking I, Dietz K, Golldack D. The Arabidopsis basic leucine zipper transcription factor AtbZIP24 regulates complex transcriptional networks involved in abiotic stress resistance. Gene. 2009;436:45-55 pubmed publisher
..The modification of transcriptional control by regulatory transcription factors provides a useful strategy for improving salt tolerance in plants. ..
Seidel T, Seefeldt B, Sauer M, Dietz K. In vivo analysis of the 2-Cys peroxiredoxin oligomeric state by two-step FRET. J Biotechnol. 2010;149:272-9 pubmed publisher
..In addition to the proof of oligomerisation in vivo, the results demonstrate the large potential of the method for investigating tripartite protein interactions in subcellular compartments and in general cell biology. ..
Ritzefeld M, Walhorn V, Kleineberg C, Bieker A, Kock K, Herrmann C, et al
. Cooperative binding of PhoB(DBD) to its cognate DNA sequence-a combined application of single-molecule and ensemble methods. Biochemistry. 2013;52:8177-86 pubmed publisher
..Consequently, it could be shown that investigating the interactions between DNA and proteins with complementary techniques is necessary to fully understand the corresponding recognition process...
Koschut D, Richert L, Pace G, Niemann H, Mely Y, Orian Rousseau V. Live cell imaging shows hepatocyte growth factor-induced Met dimerization. Biochim Biophys Acta. 2016;1863:1552-8 pubmed publisher
..The tools established in this work will be useful to further characterize the mechanism of Met activation and to define the contribution of co-receptors. ..