Experts and Doctors on chlamydomonas reinhardtii in Bochum, North Rhine Westphalia, Germany

Summary

Locale: Bochum, North Rhine Westphalia, Germany
Topic: chlamydomonas reinhardtii

Top Publications

  1. Stripp S, Sanganas O, Happe T, Haumann M. The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy. Biochemistry. 2009;48:5042-9 pubmed publisher
    ..g., to the binding of an exogenous CO at 2Fe(H) in CO-inhibited enzyme. Our XAS studies pave the way for the monitoring of atomic level structural changes at the H-cluster during H(2) catalysis...
  2. Jacobs J, Glanz S, Bunse Grassmann A, Kruse O, Kück U. RNA trans-splicing: identification of components of a putative chloroplast spliceosome. Eur J Cell Biol. 2010;89:932-9 pubmed publisher
  3. Czech I, Stripp S, Sanganas O, Leidel N, Happe T, Haumann M. The [FeFe]-hydrogenase maturation protein HydF contains a H-cluster like [4Fe4S]-2Fe site. FEBS Lett. 2011;585:225-30 pubmed publisher
    ..Presumably, only the 2Fe cluster is transferred from HydF to HydA1, thereby forming the active site...
  4. Eisenhauer K, Kuhne J, Ritter E, Berndt A, Wolf S, Freier E, et al. In channelrhodopsin-2 Glu-90 is crucial for ion selectivity and is deprotonated during the photocycle. J Biol Chem. 2012;287:6904-11 pubmed publisher
    ..By dynamic homology modeling, we further hypothesized that the conductive pore is flanked by Glu-90 and located between helices A, B, C, and G. ..
  5. Trebst A, Depka B, Holländer Czytko H. A specific role for tocopherol and of chemical singlet oxygen quenchers in the maintenance of photosystem II structure and function in Chlamydomonas reinhardtii. FEBS Lett. 2002;516:156-60 pubmed
    ..This supports the notion that 1O2 is the intermediate in light triggered D1 protein turnover. ..
  6. Hemschemeier A, Jacobs J, Happe T. Biochemical and physiological characterization of the pyruvate formate-lyase Pfl1 of Chlamydomonas reinhardtii, a typically bacterial enzyme in a eukaryotic alga. Eukaryot Cell. 2008;7:518-26 pubmed publisher
    ..1.1.1) or pyruvate ferredoxin oxidoreductase (EC 1.2.7.1). The latter enzymes might substitute for Pfl1 activity when Pfl1 is specifically inhibited by hypophosphite...
  7. Jacobs J, Pudollek S, Hemschemeier A, Happe T. A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii. FEBS Lett. 2009;583:325-9 pubmed publisher
    ..Thus, Fdx5 seems to have a special role in a yet to be identified anaerobic pathway...
  8. Winkler M, Kuhlgert S, Hippler M, Happe T. Characterization of the key step for light-driven hydrogen evolution in green algae. J Biol Chem. 2009;284:36620-7 pubmed publisher
    ..Mapping of relevant HydA1 and PetF residues constitutes an important basis for manipulating the physiological photosynthetic electron flow in favor of light-driven H(2) production. ..
  9. Lambertz C, Hemschemeier A, Happe T. Anaerobic expression of the ferredoxin-encoding FDX5 gene of Chlamydomonas reinhardtii is regulated by the Crr1 transcription factor. Eukaryot Cell. 2010;9:1747-54 pubmed publisher
    ..Moreover, in a Crr1-deficient C. reinhardtii strain, FDX5 is not expressed. These results clearly indicate that Crr1 is involved in the transcriptional regulation of the FDX5 gene in the absence of oxygen or copper...

More Information

Publications14

  1. Philipps G, Krawietz D, Hemschemeier A, Happe T. A pyruvate formate lyase-deficient Chlamydomonas reinhardtii strain provides evidence for a link between fermentation and hydrogen production in green algae. Plant J. 2011;66:330-40 pubmed publisher
    ..This analysis shows that the PFL1 pathway has a significant impact on hydrogen metabolism in C. reinhardtii. ..
  2. Pape M, Lambertz C, Happe T, Hemschemeier A. Differential expression of the Chlamydomonas [FeFe]-hydrogenase-encoding HYDA1 gene is regulated by the copper response regulator1. Plant Physiol. 2012;159:1700-12 pubmed publisher
    ..Electrophoretic mobility shift assays showed that the CRR1 SBP domain binds to one of the GTAC cores in vitro. These combined results prove that CRR1 is involved in HYDA1 promoter activation...
  3. Noth J, Krawietz D, Hemschemeier A, Happe T. Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii. J Biol Chem. 2013;288:4368-77 pubmed publisher
    ..reinhardtii PFR1 was also able to allow H(2) evolution in reaction mixtures containing Clostridium acetobutylicum 2[4Fe-4S]-ferredoxin and [Fe-Fe]-hydrogenase HYDA...
  4. Hemschemeier A, Düner M, Casero D, Merchant S, Winkler M, Happe T. Hypoxic survival requires a 2-on-2 hemoglobin in a process involving nitric oxide. Proc Natl Acad Sci U S A. 2013;110:10854-9 pubmed publisher
    ..As recombinant THB8 furthermore reacts with NO, the results of this study indicate that THB8 is part of an NO-dependent signaling pathway. ..
  5. Engelbrecht V, Rodríguez Maciá P, Esselborn J, Sawyer A, Hemschemeier A, Rüdiger O, et al. The structurally unique photosynthetic Chlorella variabilis NC64A hydrogenase does not interact with plant-type ferredoxins. Biochim Biophys Acta. 2017;1858:771-778 pubmed publisher
    ..In an electrochemical characterization, the enzyme exhibited features typical of bacterial [FeFe]-hydrogenases (e.g. minor anaerobic oxidative inactivation), as well as of algal enzymes (very high oxygen sensitivity). ..