Experts and Doctors on amino acid sequence in Wuppertal, North Rhine Westphalia, Germany
Locale: Wuppertal, North Rhine Westphalia, Germany
Topic: amino acid sequence
Freiberg C, Pohlmann J, Nell P, Endermann R, Schuhmacher J, Newton B, et al
. Novel bacterial acetyl coenzyme A carboxylase inhibitors with antibiotic efficacy in vivo. Antimicrob Agents Chemother. 2006;50:2707-12 pubmed
..The obvious improvements achieved by chemical derivatization reflect the potential of this novel antibiotic compound class for future therapy. ..
Kapoor K, Finer Moore J, Pedersen B, Caboni L, Waight A, Hillig R, et al
. Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides. Proc Natl Acad Sci U S A. 2016;113:4711-6 pubmed publisher
..The inhibitor cocomplex structures of hGLUT1 provide an important structural insight for the design of more selective inhibitors for hGLUTs and hGLUT1 in particular. ..
Seip S, Lanz R, Gutknecht R, Flükiger K, Erni B. The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit. Eur J Biochem. 1997;243:306-14 pubmed
..The protein fold of IIBLev has no similarity to any of the known structures of other phosphoenolpyruvate-dependent-carbohydrate-phosphotransferase-system proteins. ..
Rumsfeld J, Ziegelbauer K, Spaltmann F. High-throughput assay for inorganic pyrophosphatases using the cytosolic enzymes of Saccharomyces cerevisiae and human as an example. Protein Expr Purif. 2000;18:303-9 pubmed
..The resulting complex was quantified spectrophotometrically. The assay was adapted to a microtiter plate format. Thus, it is possible to screen a large compound pool for iPPase inhibitors in a short period of time. ..
Kaschabek S, Kuhn B, Müller D, Schmidt E, Reineke W. Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. 2002;184:207-15 pubmed
Hortner M, Nielsch U, Mayr L, Heinrich P, Haan S. A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor. Eur J Biochem. 2002;269:2516-26 pubmed
Oehme F, Ellinghaus P, Kolkhof P, Smith T, Ramakrishnan S, Hütter J, et al
. Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors. Biochem Biophys Res Commun. 2002;296:343-9 pubmed
..Suppression of transactivation was correlated with decrease of cellular contents of HIF. Thus, PH-4 might be related to cellular oxygen sensing. ..
Möhlmann S, Bringmann P, Greven S, Harrenga A. Site-specific modification of ED-B-targeting antibody using intein-fusion technology. BMC Biotechnol. 2011;11:76 pubmed publisher
..The described protocols can be used to prepare immunoconjugates of high homogeneity and with a defined drug load of two. The attachment to the C-termini is expected to retain the affinity and effector functions of the antibodies. ..
Hoffmann L, Schmidt P, Keim Y, Hoffmann C, Schmidt H, Stasch J. Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells. PLoS ONE. 2011;6:e23596 pubmed publisher
..Nevertheless, it allows to monitor sGC's redox regulation in living cells and future enhancements might be able to extend this approach to in vivo conditions. ..