Experts and Doctors on escherichia coli proteins in Osnabrück, Lower Saxony, Germany


Locale: Osnabrück, Lower Saxony, Germany
Topic: escherichia coli proteins

Top Publications

  1. Sippach M, Weidlich D, Klose D, Abé C, Klare J, Schneider E, et al. Conformational changes of the histidine ATP-binding cassette transporter studied by double electron-electron resonance spectroscopy. Biochim Biophys Acta. 2014;1838:1760-8 pubmed publisher
    ..Our results are in line with a rearrangement of transmembrane helices 4 and 4' of HisQM during the closed to the semi-open transition of HisP2 driven by the reorientation of the coupled helices 3a and 3b to occur upon hydrolysis. ..
  2. Jung H. Towards the molecular mechanism of Na(+)/solute symport in prokaryotes. Biochim Biophys Acta. 2001;1505:131-43 pubmed
    ..Taken together, the studies substantiate the common idea that Na(+)/solute symport is the result of a series of ligand-induced structural changes. ..
  3. Erdmann F, Jung M, Maurer P, Harsman A, Zimmermann R, Wagner R. The mammalian and yeast translocon complexes comprise a characteristic Sec61 channel. Biochem Biophys Res Commun. 2010;396:714-20 pubmed publisher
    ..We therefore propose that considerable differences between the respective eukaryote and prokaryote protein-conducting channel units and their regulation exist. ..
  4. Zimmann P, Steinbrügge A, Schniederberend M, Jung K, Altendorf K. The extension of the fourth transmembrane helix of the sensor kinase KdpD of Escherichia coli is involved in sensing. J Bacteriol. 2007;189:7326-34 pubmed
    ..These data clearly show that the extension of the fourth transmembrane helix encompassing the arginine cluster is mainly involved in sensing both K+ limitation and osmotic upshift, which may not be separated mechanistically. ..
  5. Göhler A, Staab A, Gabor E, Homann K, Klang E, Kosfeld A, et al. Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12. J Bacteriol. 2012;194:1024-35 pubmed publisher
    ..This proteolytic activity of MtfA modulated by Mlc constitutes a newly identified PTS output signal that responds to changes in environmental conditions. ..
  6. Irzik K, Pfrötzschner J, Goss T, Ahnert F, Haupt M, Greie J. The KdpC subunit of the Escherichia coli K+-transporting KdpB P-type ATPase acts as a catalytic chaperone. FEBS J. 2011;278:3041-53 pubmed publisher
  7. Heitkamp T, Kalinowski R, Bottcher B, Börsch M, Altendorf K, Greie J. K+-translocating KdpFABC P-type ATPase from Escherichia coli acts as a functional and structural dimer. Biochemistry. 2008;47:3564-75 pubmed publisher
  8. Greie J, Altendorf K. The K+-translocating KdpFABC complex from Escherichia coli: a P-type ATPase with unique features. J Bioenerg Biomembr. 2007;39:397-402 pubmed
  9. Becker D, Fendler K, Altendorf K, Greie J. The conserved dipole in transmembrane helix 5 of KdpB in the Escherichia coli KdpFABC P-type ATPase is crucial for coupling and the electrogenic K+-translocation step. Biochemistry. 2007;46:13920-8 pubmed
    ..In addition, these findings strongly suggest that the dipole residues in KdpB are not directly responsible for the characteristic electrogenic reaction step of KdpFABC, which most likely occurs within the K+-translocating KdpA subunit. ..

More Information


  1. Altendorf K, Voelkner P, Puppe W. The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coli. Res Microbiol. 1994;145:374-81 pubmed
  2. Greie J, Heitkamp T, Altendorf K. The transmembrane domain of subunit b of the Escherichia coli F1F(O) ATP synthase is sufficient for H(+)-translocating activity together with subunits a and c. Eur J Biochem. 2004;271:3036-42 pubmed
    ..Furthermore, the data obtained functionally support the monomeric NMR structure of the synthetic b(1-34). ..
  3. Heermann R, Fohrmann A, Altendorf K, Jung K. The transmembrane domains of the sensor kinase KdpD of Escherichia coli are not essential for sensing K+ limitation. Mol Microbiol. 2003;47:839-48 pubmed
  4. Stallkamp I, Altendorf K, Jung K. Amino acid replacements in transmembrane domain 1 influence osmosensing but not K+ sensing by the sensor kinase KdpD of Escherichia coli. Arch Microbiol. 2002;178:525-30 pubmed
    ..The results reveal that the osmosensing and K+ -sensing properties of KdpD can be dissected. Furthermore, the data support the hypothesis that osmosensing involves amino acid residues of the transmembrane domains. ..
  5. Gassel M, Altendorf K. Analysis of KdpC of the K(+)-transporting KdpFABC complex of Escherichia coli. Eur J Biochem. 2001;268:1772-81 pubmed
    ..A simultaneous substitution of both regions was not possible. ..
  6. Weber Sparenberg C, Pöplau P, Brookman H, Rochon M, Möckel C, Nietschke M, et al. Characterization of the type III export signal of the flagellar hook scaffolding protein FlgD of Escherichia coli. Arch Microbiol. 2006;186:307-16 pubmed
    ..Analysis of frame-shift mutations and alterations of the nucleotide sequence suggest a proteinaceous nature of the signal. Furthermore, the physicochemical properties of the first about eight amino acids are crucial for export. ..
  7. Heitkamp T, Bottcher B, Greie J. Solution structure of the KdpFABC P-type ATPase from Escherichia coli by electron microscopic single particle analysis. J Struct Biol. 2009;166:295-302 pubmed publisher
    ..Overall, the arrangement of subunits agrees with biochemical data and the predictions on subunit interactions. ..
  8. Jung H. Topology and function of the Na+/proline transporter of Escherichia coli, a member of the Na+/solute cotransporter family. Biochim Biophys Acta. 1998;1365:60-4 pubmed
    ..The functional importance of TM II is further confirmed by the observation that replacement of Arg40, Ser50, Ala53, or Ser57 alters transport kinetics dramatically. ..