Genomes and Genes
Experts and Doctors on tertiary protein structure in Berlin, Germany
Locale: Berlin, Germany
Topic: tertiary protein structure
Publications103 found, 100 shown here
- Mehlitz A, Banhart S, Mäurer A, Kaushansky A, Gordus A, Zielecki J, et al. Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1. J Cell Biol. 2010;190:143-57 pubmed publisher..trachomatis-induced cell survival and suggest that Tarp functions as a multivalent phosphorylation-dependent signaling hub that is important during the early phase of chlamydial infection. ..
- Krause A, Stoye J, Vingron M. Large scale hierarchical clustering of protein sequences. BMC Bioinformatics. 2005;6:15 pubmed..molgen.mpg.de/. Comparisons with other widely used clustering methods on various data sets show the abilities and strengths of our clustering methods in producing a biologically meaningful grouping of protein sequences. ..
- Heink S, Ludwig D, Kloetzel P, Krüger E. IFN-gamma-induced immune adaptation of the proteasome system is an accelerated and transient response. Proc Natl Acad Sci U S A. 2005;102:9241-6 pubmed
- Ullmann G, Hauswald M, Jensen A, Kostic N, Knapp E. Comparison of the physiologically equivalent proteins cytochrome c6 and plastocyanin on the basis of their electrostatic potentials. Tryptophan 63 in cytochrome c6 may be isofunctional with tyrosine 83 in plastocyanin. Biochemistry. 1997;36:16187-96 pubmed..Now we corroborate this proposal by analyzing available amino acid sequences. ..
- Faelber K, Kirchhofer D, Presta L, Kelley R, Muller Y. The 1.85 A resolution crystal structures of tissue factor in complex with humanized Fab D3h44 and of free humanized Fab D3h44: revisiting the solvation of antigen combining sites. J Mol Biol. 2001;313:83-97 pubmed..A strategy is proposed on how to exploit this high resolution structural data to guide the affinity maturation of humanised antibodies. ..
- Schwabe G, Turkmen S, Leschik G, Palanduz S, Stöver B, Goecke T, et al. Brachydactyly type C caused by a homozygous missense mutation in the prodomain of CDMP1. Am J Med Genet A. 2004;124A:356-63 pubmed..The mutation described here points toward an important function of the prodomain for the folding, secretion, and availability of biologically active CDMP1. ..
- Landgraf C, Panni S, Montecchi Palazzi L, Castagnoli L, Schneider Mergener J, Volkmer Engert R, et al. Protein interaction networks by proteome peptide scanning. PLoS Biol. 2004;2:E14 pubmed..WISE can be easily extended to a variety of protein interaction domains, including those binding to modified peptides, thereby offering a powerful proteomic tool to help completing a full description of the cell interactome. ..
- Staub E, Fiziev P, Rosenthal A, Hinzmann B. Insights into the evolution of the nucleolus by an analysis of its protein domain repertoire. Bioessays. 2004;26:567-81 pubmed..Supplementary material for this article can be found on the BioEssays website at http://www.interscience.wiley.com/jpages/0265-9247/suppmat/index.html. ..
- Kuhn H, Walther M, Kuban R. Mammalian arachidonate 15-lipoxygenases structure, function, and biological implications. Prostaglandins Other Lipid Mediat. 2002;68-69:263-90 pubmed..In addition, the major hypotheses on the physiological and patho-physiological roles of 15-LOXs will be discussed briefly. ..
- Wüller S, Wiesner B, Löffler A, Furkert J, Krause G, Hermosilla R, et al. Pharmacochaperones post-translationally enhance cell surface expression by increasing conformational stability of wild-type and mutant vasopressin V2 receptors. J Biol Chem. 2004;279:47254-63 pubmed..The mechanisms involved in successful rescue of cell surface delivery are explained in a three-dimensional homology model of the antagonist-bound hV2R. ..
- Kofler M, Motzny K, Beyermann M, Freund C. Novel interaction partners of the CD2BP2-GYF domain. J Biol Chem. 2005;280:33397-402 pubmed..A direct interaction of the CD2BP2-GYF domain with the novel protein interaction partners PI31 and NPWBP was verified by yeast two-hybrid analysis. ..
- Mankertz J, Waller J, Hillenbrand B, Tavalali S, Florian P, Schoneberg T, et al. Gene expression of the tight junction protein occludin includes differential splicing and alternative promoter usage. Biochem Biophys Res Commun. 2002;298:657-66 pubmed..Gene expression mediated by this promoter is influenced by the pro-inflammatory cytokine tumor necrosis factor alpha. ..
- Weber H, Bernhardt A, Dieterle M, Hano P, Mutlu A, Estelle M, et al. Arabidopsis AtCUL3a and AtCUL3b form complexes with members of the BTB/POZ-MATH protein family. Plant Physiol. 2005;137:83-93 pubmed..The results presented here provide strong evidence that AtCUL3a and AtCUL3b can assemble in Arabidopsis with BTB/POZ-MATH and AtRBX1 proteins to form functional E3 ligases. ..
- Rao Y, Ma Q, Vahedi Faridi A, Sundborger A, Pechstein A, Puchkov D, et al. Molecular basis for SH3 domain regulation of F-BAR-mediated membrane deformation. Proc Natl Acad Sci U S A. 2010;107:8213-8 pubmed publisher..Our data thus suggest a structure-based model for SH3-mediated regulation of BAR/F-BAR domain function. ..
- Grantcharova E, Furkert J, Reusch H, Krell H, Papsdorf G, Beyermann M, et al. The extracellular N terminus of the endothelin B (ETB) receptor is cleaved by a metalloprotease in an agonist-dependent process. J Biol Chem. 2002;277:43933-41 pubmed..This is the first example of a multispanning membrane protein, which is cleaved by a metalloprotease, but retains its functional activity and overall structure. ..
- Buscher K, Riese S, Shakibaei M, Reich C, Dernedde J, Tauber R, et al. The transmembrane domains of L-selectin and CD44 regulate receptor cell surface positioning and leukocyte adhesion under flow. J Biol Chem. 2010;285:13490-7 pubmed publisher..These findings demonstrate that TM domains of L-selectin and CD44 play a crucial role in cell adhesion under flow by targeting receptors to microvilli or the cell body, respectively. ..
- Alder Baerens N, Muller P, Pohl A, Korte T, Hamon Y, Chimini G, et al. Headgroup-specific exposure of phospholipids in ABCA1-expressing cells. J Biol Chem. 2005;280:26321-9 pubmed..In control cells, however, fluid-phase endocytosis but not receptor-mediated endocytosis was significantly reduced upon glyburide treatment...
- Voigt P, Dorner M, Schaefer M. Characterization of p87PIKAP, a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B. J Biol Chem. 2006;281:9977-86 pubmed
- Kolbeck B, May P, Schmidt Goenner T, Steinke T, Knapp E. Connectivity independent protein-structure alignment: a hierarchical approach. BMC Bioinformatics. 2006;7:510 pubmed..GANGSTA is able to detect structural similarity of protein folds that are assigned to different superfamilies but nevertheless possess similar structures and perform related functions, even if these proteins differ in SSE connectivity. ..
- Glienke J, Fenten G, Seemann M, Sturz A, Thierauch K. Human SPRY2 inhibits FGF2 signalling by a secreted factor. Mech Dev. 2000;96:91-9 pubmed
- Tegethoff S, Behlke J, Scheidereit C. Tetrameric oligomerization of IkappaB kinase gamma (IKKgamma) is obligatory for IKK complex activity and NF-kappaB activation. Mol Cell Biol. 2003;23:2029-41 pubmed..These data thus suggest that IKKgamma tetramerization enforces a spatial positioning of two kinase dimers to facilitate transautophosphorylation and activation. ..
- Jung R, Wendeler M, Danevad M, Himmelbauer H, Gessner R. Phylogenetic origin of LI-cadherin revealed by protein and gene structure analysis. Cell Mol Life Sci. 2004;61:1157-66 pubmed..These findings strengthen our assumption that LI-cadherin originated from an ancestral cadherin with five domains by a partial gene duplication event. ..
- Ludewig A, Kober Eisermann C, Weitzel C, Bethke A, Neubert K, Gerisch B, et al. A novel nuclear receptor/coregulator complex controls C. elegans lipid metabolism, larval development, and aging. Genes Dev. 2004;18:2120-33 pubmed..We propose that the DIN-1S/DAF-12 complex serves as a molecular switch that implements slow life history alternatives in response to diminished hormonal signals. ..
- Langenickel T, Buttgereit J, Pagel I, Dietz R, Willenbrock R, Bader M. Forced homodimerization by site-directed mutagenesis alters guanylyl cyclase activity of natriuretic peptide receptor B. Hypertension. 2004;43:460-5 pubmed..Localization of the crosslink in relation to the cell membrane is important for configuration of the extracellular domain and the consecutive signal transduction. ..
- Rutz C, Renner A, Alken M, Schulz K, Beyermann M, Wiesner B, et al. The corticotropin-releasing factor receptor type 2a contains an N-terminal pseudo signal peptide. J Biol Chem. 2006;281:24910-21 pubmed..Our data also show that the extreme N termini of the individual CRF receptor subtypes differ substantially. ..
- Schuler M, Connell S, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, et al. Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nat Struct Mol Biol. 2006;13:1092-6 pubmed..Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation. ..
- Kofler M, Heuer K, Zech T, Freund C. Recognition sequences for the GYF domain reveal a possible spliceosomal function of CD2BP2. J Biol Chem. 2004;279:28292-7 pubmed..The colocalization of CD2BP2 and SmB proteins in the nucleus of Jurkat T cells and HeLa cells suggests a function of the GYF domain of CD2BP2 in mediating protein-protein interactions within the spliceosome. ..
- Opitz B, Püschel A, Schmeck B, Hocke A, Rosseau S, Hammerschmidt S, et al. Nucleotide-binding oligomerization domain proteins are innate immune receptors for internalized Streptococcus pneumoniae. J Biol Chem. 2004;279:36426-32 pubmed..pneumoniae. Furthermore, Rip-2 and members of the Toll-like receptor-signaling cascade are involved in the Nod2-dependent activation of NF-kappaB induced by pneumococci. ..
- Reiche J, Theilig F, Rafiqi F, Carlo A, Militz D, Mutig K, et al. SORLA/SORL1 functionally interacts with SPAK to control renal activation of Na(+)-K(+)-Cl(-) cotransporter 2. Mol Cell Biol. 2010;30:3027-37 pubmed publisher..Our data suggest a novel regulatory pathway whereby intracellular trafficking of SPAK by the sorting receptor SORLA is crucial for proper NKCC2 activation and for maintenance of renal ion balance. ..
- Schultz J. HTTM, a horizontally transferred transmembrane domain. Trends Biochem Sci. 2004;29:4-7 pubmed..The conservation of VKGC catalytic sites also indicates an enzymatic function for the other family members. ..
- Pless O, Kowenz Leutz E, Knoblich M, Lausen J, Beyermann M, Walsh M, et al. G9a-mediated lysine methylation alters the function of CCAAT/enhancer-binding protein-beta. J Biol Chem. 2008;283:26357-63 pubmed publisher..Our data identify C/EBPbeta as a direct substrate of G9a-mediated post-translational modification that alters the functional properties of C/EBPbeta during gene regulation. ..
- Jurchott K, Bergmann S, Stein U, Walther W, Janz M, Manni I, et al. YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression. J Biol Chem. 2003;278:27988-96 pubmed..Thus, we have identified YB-1 as a cell cycle stage-specific transcription factor important for cell proliferation. ..
- Macias M, Gervais V, Civera C, Oschkinat H. Structural analysis of WW domains and design of a WW prototype. Nat Struct Biol. 2000;7:375-9 pubmed
- Preissner S, Kroll K, Dunkel M, Senger C, Goldsobel G, Kuzman D, et al. SuperCYP: a comprehensive database on Cytochrome P450 enzymes including a tool for analysis of CYP-drug interactions. Nucleic Acids Res. 2010;38:D237-43 pubmed publisher..SuperCYP is meant to be a platform and a starting point for scientists and health professionals for furthering their research. ..
- von Kries J, Winbeck G, Asbrand C, Schwarz Romond T, Sochnikova N, Dell Oro A, et al. Hot spots in beta-catenin for interactions with LEF-1, conductin and APC. Nat Struct Biol. 2000;7:800-7 pubmed..Binding of APC to conductin/axin activates the latter and occurs between their SAMP and RGS domains, respectively. ..
- Bojarski C, Weiske J, Schoneberg T, Schröder W, Mankertz J, Schulzke J, et al. The specific fates of tight junction proteins in apoptotic epithelial cells. J Cell Sci. 2004;117:2097-107 pubmed..While the amount of claudin-2 protein was reduced similarly to occludin, ZO-1 and ZO-2, claudin-1 was either fully preserved or was even increased in apoptotic cells. ..
- Nonhoff U, Ralser M, Welzel F, Piccini I, Balzereit D, Yaspo M, et al. Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules. Mol Biol Cell. 2007;18:1385-96 pubmed..Thus, our data imply that the cellular ataxin-2 concentration is important for the assembly of stress granules and P-bodies, which are main compartments for regulating and controlling mRNA degradation, stability, and translation. ..
- Max K, Wunderlich M, Roske Y, Schmid F, Heinemann U. Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability. J Mol Biol. 2007;369:1087-97 pubmed publisher..The stabilizing mutations are clustered at a contiguous surface area of Bs-CspB, which apparently is critically important for the stability of the beta-barrel structure but not well optimized in the wild-type protein...
- Richter G, Hein G, Blankenstein T, Diamantstein T. The rat interleukin 4 receptor: coevolution of ligand and receptor. Cytokine. 1995;7:237-41 pubmed
- Veit M. The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues. Biochem J. 2004;384:233-7 pubmed..The N-terminal domain of human Ykt6 contains a Pal-CoA binding site and is required for the reaction. ..
- Voigt P, Brock C, Nurnberg B, Schaefer M. Assigning functional domains within the p101 regulatory subunit of phosphoinositide 3-kinase gamma. J Biol Chem. 2005;280:5121-7 pubmed..In addition to establishing a domain structure for p101, our results point to the existence of a previously unknown, p101-related regulatory subunit for PI3Kgamma. ..
- Rückert C, Fiorillo M, Loll B, Moretti R, Biesiadka J, Saenger W, et al. Conformational dimorphism of self-peptides and molecular mimicry in a disease-associated HLA-B27 subtype. J Biol Chem. 2006;281:2306-16 pubmed..These results lend support to the concept that conformational dimorphisms of MHC class I-bound peptides might be connected with the occurrence of self-reactive CTL. ..
- Schulze A, Standera S, Buerger E, Kikkert M, van Voorden S, Wiertz E, et al. The ubiquitin-domain protein HERP forms a complex with components of the endoplasmic reticulum associated degradation pathway. J Mol Biol. 2005;354:1021-7 pubmed..We propose that ubiquitylation, as well as retro-translocation of proteins from the ER are performed by this modular protein complex, which permits the close coordination of these consecutive steps within ERAD. ..
- Kaden D, Munter L, Joshi M, Treiber C, Weise C, Bethge T, et al. Homophilic interactions of the amyloid precursor protein (APP) ectodomain are regulated by the loop region and affect beta-secretase cleavage of APP. J Biol Chem. 2008;283:7271-9 pubmed publisher..We suggest that APP acquires a loop-mediated homodimeric state that is further stabilized by interactions of hydrophobic residues of neighboring domains. ..
- Wolff T, Unterstab G, Heins G, Richt J, Kann M. Characterization of an unusual importin alpha binding motif in the borna disease virus p10 protein that directs nuclear import. J Biol Chem. 2002;277:12151-7 pubmed
- Borchert A, Savaskan N, Kuhn H. Regulation of expression of the phospholipid hydroperoxide/sperm nucleus glutathione peroxidase gene. Tissue-specific expression pattern and identification of functional cis- and trans-regulatory elements. J Biol Chem. 2003;278:2571-80 pubmed..The decision, which GPx isoform is expressed in a given cell, appears to be made by alternative splicing of a joint primary transcript. ..
- Manjasetty B, Niesen F, Scheich C, Roske Y, Goetz F, Behlke J, et al. X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1). BMC Struct Biol. 2005;5:21 pubmed..However, it remains to be established whether the rather weak dimerisation of APEG-1 involving this motif is physiologically relevant. ..
- Andersen O, Schmidt V, Spoelgen R, Gliemann J, Behlke J, Galatis D, et al. Molecular dissection of the interaction between amyloid precursor protein and its neuronal trafficking receptor SorLA/LR11. Biochemistry. 2006;45:2618-28 pubmed..These data shed new light on the molecular determinants of neuronal APP trafficking and processing and on possible targets for intervention with senile plaque formation in patients with AD. ..
- Maritzen T, Podufall J, Haucke V. Stonins--specialized adaptors for synaptic vesicle recycling and beyond?. Traffic. 2010;11:8-15 pubmed publisher..In this review, we summarize the current state of knowledge about mammalian stonins with a special focus on the role of stonin 2 in SV recycling at presynaptic nerve terminals. ..
- Patzke C, Max K, Behlke J, Schreiber J, Schmidt H, Dorner A, et al. The coxsackievirus-adenovirus receptor reveals complex homophilic and heterophilic interactions on neural cells. J Neurosci. 2010;30:2897-910 pubmed publisher..The results of these functional and binding studies are correlated to a U-shaped homodimer of the complete extracellular domains of CAR detected by x-ray crystallography. ..
- Ash M, Faelber K, Kosslick D, Albert G, Roske Y, Kofler M, et al. Conserved beta-hairpin recognition by the GYF domains of Smy2 and GIGYF2 in mRNA surveillance and vesicular transport complexes. Structure. 2010;18:944-54 pubmed publisher..The current study highlights the structural basis for PRS recognition by Smy2-type GYF domains, and implicates Smy2 and GIGYF2 in both mRNA processing and the secretory pathway. ..
- Schmidt A, Utepbergenov D, Krause G, Blasig I. Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludin. Biochem Biophys Res Commun. 2001;288:1194-9 pubmed..We demonstrate the use of SPR spectroscopy as an effective approach for characterisation of the interactions of junction proteins. ..
- Schultz Heienbrok R, Maier T, Strater N. A large hinge bending domain rotation is necessary for the catalytic function of Escherichia coli 5'-nucleotidase. Biochemistry. 2005;44:2244-52 pubmed..The kinetic, spectroscopic, and theoretical data are brought together to discuss the domain rotation in terms of the kinetic functioning of E. coli 5'-nucleotidase. ..
- Connell S, Takemoto C, Wilson D, Wang H, Murayama K, Terada T, et al. Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors. Mol Cell. 2007;25:751-64 pubmed..Therefore, a network of interaction with the ribosome establishes the active GTP conformation of EF-G and thus facilitates GTP hydrolysis and tRNA translocation. ..
- Rossmann M, Schultz Heienbrok R, Behlke J, Remmel N, Alings C, Sandhoff K, et al. Crystal structures of human saposins C andD: implications for lipid recognition and membrane interactions. Structure. 2008;16:809-17 pubmed publisher..The crystal structure of SapD suggests that side chains of Lys10 and Arg17 are involved in initial association with the preferred anionic biological membranes by forming salt bridges with sulfate or phosphate lipid headgroups. ..
- Schubert M, Oschkinat H, Schmieder P. Amino acid type-selective backbone 1H-15N-correlations for Arg and Lys. J Biomol NMR. 2001;20:379-84 pubmed..The new experiments are recorded as two-dimensional 1H-15N-correlations and their performance is demonstrated with the application to a protein domain of 83 amino acids...
- Geier C, Perrot A, Ozcelik C, Binner P, Counsell D, Hoffmann K, et al. Mutations in the human muscle LIM protein gene in families with hypertrophic cardiomyopathy. Circulation. 2003;107:1390-5 pubmed..Family studies revealed cosegregation of clinically affected individuals with the respective mutations in MLP. Here, we present evidence that mutations in the CRP3/MLP gene can cause HCM. ..
- Bannert N, Vollhardt K, Asomuddinov B, Haag M, König H, Norley S, et al. PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits. J Biol Chem. 2003;278:42190-9 pubmed..Alternatively, an intracellular complex of npro-IL-16, ion channels, and components of myosin motors in neurons suggests a role in protein targeting. ..
- Haase H, Pagel I, Khalina Y, Zacharzowsky U, Person V, Lutsch G, et al. The carboxyl-terminal ahnak domain induces actin bundling and stabilizes muscle contraction. FASEB J. 2004;18:839-41 pubmed..Our results suggest that the carboxyl-terminal ahnak domain exerts a stabilizing effect on muscle contractility via its interaction with actin of thin filaments. ..
- Rüder C, Reimer T, Delgado Martinez I, Hermosilla R, Engelsberg A, Nehring R, et al. EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin. Mol Biol Cell. 2005;16:1245-57 pubmed..The conjunction between EBAG9 and Snapin adds an additional layer of control on exocytosis processes; in addition, mechanistic evidence is provided that inhibition of phosphorylation has a regulatory function in exocytosis. ..
- Mo X, Kowenz Leutz E, Laumonnier Y, Xu H, Leutz A. Histone H3 tail positioning and acetylation by the c-Myb but not the v-Myb DNA-binding SANT domain. Genes Dev. 2005;19:2447-57 pubmed..Our data link a novel chromatin function of c-Myb with lineage-specific expression of differentiation genes and relate the loss of this function with the leukemic conversion of Myb...
- Agarwal N, Hardt T, Brero A, Nowak D, Rothbauer U, Becker A, et al. MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation. Nucleic Acids Res. 2007;35:5402-8 pubmed..We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation. ..
- Maritzen T, Schmidt M, Kukhtina V, Higman V, Strauss H, Volkmer R, et al. A novel subtype of AP-1-binding motif within the palmitoylated trans-Golgi network/endosomal accessory protein Gadkin/gamma-BAR. J Biol Chem. 2010;285:4074-86 pubmed publisher..Our studies thus unravel the molecular basis for Gadkin-mediated AP-1 recruitment to TGN/endosomal membranes and identify a novel subtype of the AP-1-binding motif. ..
- Rose A, Goede A, Hildebrand P. MPlot--a server to analyze and visualize tertiary structure contacts and geometrical features of helical membrane proteins. Nucleic Acids Res. 2010;38:W602-8 pubmed publisher..URL: http://proteinformatics.charite.de/mplot/. ..
- Petersen H, Hilpert J, Jacobsen C, Lauwers A, Roebroek A, Willnow T. Low-density lipoprotein receptor-related protein interacts with MafB, a regulator of hindbrain development. FEBS Lett. 2004;565:23-7 pubmed..The ICD co-localizes with MafB to the nucleus and negatively regulates its transcriptional activity, suggesting a possible role for LRP in brain development. ..
- Kofler M, Motzny K, Freund C. GYF domain proteomics reveals interaction sites in known and novel target proteins. Mol Cell Proteomics. 2005;4:1797-811 pubmed..The binding epitope of the GYF domain from the yeast SMY2 protein was mapped by NMR spectroscopy and led to a structural model that accounts for the different binding properties of SMY2-type GYF domains and the CD2BP2-GYF domain. ..
- Jehle S, van Rossum B, Stout J, Noguchi S, Falber K, Rehbein K, et al. alphaB-crystallin: a hybrid solid-state/solution-state NMR investigation reveals structural aspects of the heterogeneous oligomer. J Mol Biol. 2009;385:1481-97 pubmed publisher..8. This steep pH-dependent switch may be important for alphaB to function optimally (e.g., to preserve the filament integrity of cardiac muscle proteins such as actin and desmin during cardiac ischemia, which is accompanied by acidosis). ..
- Kahlfeldt N, Vahedi Faridi A, Koo S, Schäfer J, Krainer G, Keller S, et al. Molecular basis for association of PIPKI gamma-p90 with clathrin adaptor AP-2. J Biol Chem. 2010;285:2734-49 pubmed publisher..We postulate a model according to which multiple interactions between PIPKI gamma-p90 and AP-2 lead to spatiotemporally controlled PI(4,5)P(2) synthesis during clathrin-mediated SV endocytosis. ..
- Kerst B, Mennerich D, Schuelke M, Stoltenburg Didinger G, von Moers A, Gossrau R, et al. Heterozygous myogenic factor 6 mutation associated with myopathy and severe course of Becker muscular dystrophy. Neuromuscul Disord. 2000;10:572-7 pubmed..This mutation is normally associated with a mild to moderate course of Becker muscular dystrophy but the father suffered from a severe course of Becker muscular dystrophy suggesting MYF6 as a modifier. ..
- Weger S, Hammer E, Engstler M. The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation. Exp Cell Res. 2003;290:13-27 pubmed..One SUMO-1 acceptor site at lysine residue 560 could be identified within this region. However, sumolation-deficient Topors mutants showed that sumolation obviously is not required for localization to nuclear speckles. ..
- Kottwitz D, Kukhtina V, Dergousova N, Alexeev T, Utkin Y, Tsetlin V, et al. Intracellular domains of the delta-subunits of Torpedo and rat acetylcholine receptors--expression, purification, and characterization. Protein Expr Purif. 2004;38:237-47 pubmed..The results suggest that the spatial structure of the intracellular loops is dependent on environment and species, but is not changed significantly upon enzymatic phosphorylation...
- Ruehl M, Erben U, Schuppan D, Wagner C, Zeller A, Freise C, et al. The elongated first fibronectin type III domain of collagen XIV is an inducer of quiescence and differentiation in fibroblasts and preadipocytes. J Biol Chem. 2005;280:38537-43 pubmed..Based on these finding the development of small molecular analogs that modulate fibroblast cell growth and differentiation, e.g. in wound healing and fibrosis, seems feasible. ..
- Loll B, Kern J, Zouni A, Saenger W, Biesiadka J, Irrgang K. The antenna system of photosystem II from Thermosynechococcus elongatus at 3.2 A resolution. Photosynth Res. 2005;86:175-84 pubmed..The chlorophyll a in the lumenal layer are less well conserved between Photosystems I and II and even between CP43 and CP47 with 4 chlorophyll a in the former and 7 in the latter. ..
- Hebbeln P, Rodionov D, Alfandega A, Eitinger T. Biotin uptake in prokaryotes by solute transporters with an optional ATP-binding cassette-containing module. Proc Natl Acad Sci U S A. 2007;104:2909-14 pubmed..The results substantiate our earlier suggestion of a mechanistically novel group of membrane transporters...
- Thaa B, Herrmann A, Veit M. The polybasic region is not essential for membrane binding of the matrix protein M1 of influenza virus. Virology. 2009;383:150-5 pubmed publisher..We therefore suppose that M1 probably interacts with membranes via multiple binding sites. ..
- Schaper S, Nardmann J, L der G, Lurz R, Speck C, Messer W. Identification of the chromosomal replication origin from Thermus thermophilus and its interaction with the replication initiator DnaA. J Mol Biol. 2000;299:655-65 pubmed publisher..Furthermore, high-affinity DNA-binding of Tth-DnaA is dependent on the presence of ATP. The Thermus DnaA/oriC interaction will be compared with oriC complex formation generated by other DnaA proteins...
- Leitner D, Wahl M, Labudde D, Krause G, Diehl A, Schmieder P, et al. The solution structure of an N-terminally truncated version of the yeast CDC24p PB1 domain shows a different beta-sheet topology. FEBS Lett. 2005;579:3534-8 pubmed..Residues which are important for the heterodimerization with BEM1p are structurally preserved. ..
- Hoppe B, Heymann G, Kiesewetter H, Salama A. Identification and characterization of a novel HLA-DRB1 allele, DRB1*0830*. Tissue Antigens. 2005;66:160-2 pubmed..Allele-specific DNA sequencing confirmed a 286 T-->A substitution resulting in Phe67Ile. ..
- Kümmel D, Heinemann U, Veit M. Unique self-palmitoylation activity of the transport protein particle component Bet3: a mechanism required for protein stability. Proc Natl Acad Sci U S A. 2006;103:12701-6 pubmed..As a consequence of its structural defect nonacylated Bet3 does not bind to TPC6, a further subunit of the transport protein particle complex, and is degraded inside cells. ..
- Strödicke M, Karberg S, Korge G. Domina (Dom), a new Drosophila member of the FKH/WH gene family, affects morphogenesis and is a suppressor of position-effect variegation. Mech Dev. 2000;96:67-78 pubmed..The chromatin modifying capability of DOM is probably based on the FKH/WH domain, which shows a remarkable structural similarity to the winged-helix structures of H1 and the central globular domain of H5. ..
- Schleicher E, Kowalczyk R, Kay C, Hegemann P, Bacher A, Fischer M, et al. On the reaction mechanism of adduct formation in LOV domains of the plant blue-light receptor phototropin. J Am Chem Soc. 2004;126:11067-76 pubmed..In light of these observations, we discuss a radical-pair reaction mechanism for the primary photoreaction in LOV domains...
- Schwieger I, Lautz K, Krause E, Rosenthal W, Wiesner B, Hermosilla R. Derlin-1 and p97/valosin-containing protein mediate the endoplasmic reticulum-associated degradation of human V2 vasopressin receptors. Mol Pharmacol. 2008;73:697-708 pubmed..Our results demonstrate the presence of a Derlin-1-mediated ERAD pathway degrading wild-type and disease-causing V2R mutants with different misfolded domains in a mammalian system. ..
- van Wijk N, Witte F, Feike A, Schambony A, Birchmeier W, Mundlos S, et al. The LIM domain protein Wtip interacts with the receptor tyrosine kinase Ror2 and inhibits canonical Wnt signalling. Biochem Biophys Res Commun. 2009;390:211-6 pubmed publisher..Both genes and proteins show overlapping expression in the mouse embryo. We show that Wtip is able to inhibit canonical Wnt signalling in mammalian cells and in Xenopus embryos linking Wtip to a crucial developmental pathway. ..
- Hundsrucker C, Skroblin P, Christian F, Zenn H, Popara V, Joshi M, et al. Glycogen synthase kinase 3beta interaction protein functions as an A-kinase anchoring protein. J Biol Chem. 2010;285:5507-21 pubmed publisher..We show here that this domain of GSKIP and its vertebrate orthologues binds both PKA and GSK3beta and thereby provides a mechanism for the integration of PKA and GSK3beta signaling pathways. ..
- Schwabe G, Tinschert S, Buschow C, Meinecke P, Wolff G, Gillessen Kaesbach G, et al. Distinct mutations in the receptor tyrosine kinase gene ROR2 cause brachydactyly type B. Am J Hum Genet. 2000;67:822-31 pubmed..The BDB phenotype, as well as the location and the nature of the BDB mutations, suggests a specific mutational effect that cannot be explained by simple haploinsufficiency and that is distinct from that in Robinow syndrome...
- Ansieau S, Leutz A. The conserved Mynd domain of BS69 binds cellular and oncoviral proteins through a common PXLXP motif. J Biol Chem. 2002;277:4906-10 pubmed..Because deletions in E1A or EBNA2 that cover the PXLXP motifs are non-transforming, our observations suggest a role for BS69 in cell growth control that is reminiscent of abrogation of the Rb function by various oncoproteins. ..
- Kilian K, Dernedde J, Mueller E, Bahr I, Tauber R. The interaction of protein kinase C isozymes alpha, iota, and theta with the cytoplasmic domain of L-selectin is modulated by phosphorylation of the receptor. J Biol Chem. 2004;279:34472-80 pubmed..The phosphorylation-regulated association of PKC isozymes with the cytoplasmic domain of L-selectin indicates an important role of this kinase family in L-selectin signal transduction. ..
- Jung N, Wienisch M, Gu M, Rand J, Müller S, Krause G, et al. Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2. J Cell Biol. 2007;179:1497-510 pubmed publisher..Synaptotagmin-binding-defective UNC-41B is unable to rescue paralysis in C. elegans stonin mutant animals, suggesting that the mechanism of stonin-mediated SV cargo recognition is conserved from worms to mammals. ..