Genomes and Genes
Experts and Doctors on protein transport in Berlin, Germany
Locale: Berlin, Germany
Topic: protein transport
- Vehring S, Pakkiri L, Schröer A, Alder Baerens N, Herrmann A, Menon A, et al. Flip-flop of fluorescently labeled phospholipids in proteoliposomes reconstituted with Saccharomyces cerevisiae microsomal proteins. Eukaryot Cell. 2007;6:1625-34 pubmed..Our work defines yeast as a promising model system for future attempts to identify the ER phospholipid flippase and to test and purify candidate flippases...
- Mousa S, Shaqura M, Khalefa B, Zöllner C, Schaad L, Schneider J, et al. Rab7 silencing prevents ?-opioid receptor lysosomal targeting and rescues opioid responsiveness to strengthen diabetic neuropathic pain therapy. Diabetes. 2013;62:1308-19 pubmed publisher..This is in contrast to peripheral sensory neuron MOR upregulation and antinociception in inflammatory pain, and provides intriguing evidence that regulation of opioid responsiveness varies as a function of pain pathogenesis. ..
- Peth A, Boettcher J, Dubiel W. Ubiquitin-dependent proteolysis of the microtubule end-binding protein 1, EB1, is controlled by the COP9 signalosome: possible consequences for microtubule filament stability. J Mol Biol. 2007;368:550-63 pubmed..A nocodazole-induced cell-cycle arrest was partially rescued by CSN1 or EB1. These data demonstrate that the CSN-dependent protection of EB1 is important for microtubule function. ..
- Krauss M, Haucke V. Phosphoinositide-metabolizing enzymes at the interface between membrane traffic and cell signalling. EMBO Rep. 2007;8:241-6 pubmed
- Neuber A, Franke J, Wittstruck A, Schlenstedt G, Sommer T, Stade K. Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast. Mol Cell Biol. 2008;28:5348-58 pubmed publisher..In addition, we find a subpopulation of Xpo1 localized at the SPB. Based on these data, we propose a functional link between Xpo1 and the SPB and discuss a role for this exportin in spindle biogenesis in budding yeast. ..
- Gunzel D, Stuiver M, Kausalya P, Haisch L, Krug S, Rosenthal R, et al. Claudin-10 exists in six alternatively spliced isoforms that exhibit distinct localization and function. J Cell Sci. 2009;122:1507-17 pubmed publisher..The extent of all these effects strongly depended on the endogenous claudins of the transfected cells. ..
- Matz J, Matuschewski K, Kooij T. Two putative protein export regulators promote Plasmodium blood stage development in vivo. Mol Biochem Parasitol. 2013;191:44-52 pubmed publisher..Together, our results indicate auxiliary roles for TRX2 and PTEX88 and central roles for EXP2, PTEX150, and HSP101 during P. berghei blood infection. ..
- Brueckl C, Kaestle S, Kerem A, Habazettl H, Krombach F, Kuppe H, et al. Hyperoxia-induced reactive oxygen species formation in pulmonary capillary endothelial cells in situ. Am J Respir Cell Mol Biol. 2006;34:453-63 pubmed..Our findings demonstrate rapid activation of ECs by hyperoxia in situ and identify mechanisms that may be relevant in the initiation of hyperoxic lung injury. ..
- Hu Y, Warnatz H, Vanhecke D, Wagner F, Fiebitz A, Thamm S, et al. Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins. BMC Genomics. 2006;7:155 pubmed..The results from this study reveal novel functional features of human Chr21 proteins, which should contribute to further understanding of the molecular pathology of Down's syndrome. ..
- Liesche J, He H, Grimm B, Schulz A, Kuhn C. Recycling of Solanum sucrose transporters expressed in yeast, tobacco, and in mature phloem sieve elements. Mol Plant. 2010;3:1064-74 pubmed publisher..The results provide new insight into the regulation of sucrose transport and the mechanism of endocytosis in plant cells. ..
- Herda S, Raczkowski F, Mittrücker H, Willimsky G, Gerlach K, Kuhl A, et al. The sorting receptor Sortilin exhibits a dual function in exocytic trafficking of interferon-? and granzyme A in T cells. Immunity. 2012;37:854-66 pubmed publisher..Our results suggest that Sortilin modulates systemic immune responses through exocytic sorting of immunological effector molecules. ..
- Holland C, Mak T, Zimny Arndt U, Schmid M, Meyer T, Jungblut P, et al. Proteomic identification of secreted proteins of Propionibacterium acnes. BMC Microbiol. 2010;10:230 pubmed publisher..acnes virulence factors remains scarce. Here, we identified proteins secreted during anaerobic cultivation of a range of skin and clinical P. acnes isolates, spanning the four known phylogenetic groups...
- Alabert C, Bukowski Wills J, Lee S, Kustatscher G, Nakamura K, de Lima Alves F, et al. Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components. Nat Cell Biol. 2014;16:281-93 pubmed publisher..Together, this provides an extensive resource to understand genome and epigenome maintenance. ..
- Brackmann M, Schuchmann S, Anand R, Braunewell K. Neuronal Ca2+ sensor protein VILIP-1 affects cGMP signalling of guanylyl cyclase B by regulating clathrin-dependent receptor recycling in hippocampal neurons. J Cell Sci. 2005;118:2495-505 pubmed..g. by influencing cGMP-signalling. ..
- Rosenthal R, Milatz S, Krug S, Oelrich B, Schulzke J, Amasheh S, et al. Claudin-2, a component of the tight junction, forms a paracellular water channel. J Cell Sci. 2010;123:1913-21 pubmed publisher..By contrast, claudin-10b transfection did not alter water flux. We conclude that claudin-2, but not claudin-10b, forms a paracellular water channel and thus mediates paracellular water transport in leaky epithelia. ..
- Franco I, Gulluni F, Campa C, Costa C, Margaria J, Ciraolo E, et al. PI3K class II ? controls spatially restricted endosomal PtdIns3P and Rab11 activation to promote primary cilium function. Dev Cell. 2014;28:647-58 pubmed publisher..Thus, PI3K-C2? regulates the formation of a PtdIns3P pool at the PRE required for Rab11 and Shh pathway activation. ..
- Rao Y, Rückert C, Saenger W, Haucke V. The early steps of endocytosis: from cargo selection to membrane deformation. Eur J Cell Biol. 2012;91:226-33 pubmed publisher..Here we summarize recent insights gathered from a combination of structural, biochemical, and cell biological studies that have revealed a remarkable complexity of the machinery for endocytic sorting and membrane deformation. ..
- Schmidt V, Sporbert A, Rohe M, Reimer T, Rehm A, Andersen O, et al. SorLA/LR11 regulates processing of amyloid precursor protein via interaction with adaptors GGA and PACS-1. J Biol Chem. 2007;282:32956-64 pubmed..Thus, our findings identified altered routing of sorLA as a major cellular mechanism contributing to abnormal APP processing and enhanced amyloid beta-peptide formation. ..
- Grosse G, Eulitz D, Thiele T, Pahner I, Schröter S, Takamori S, et al. Axonal sorting of Kir3.3 defines a GABA-containing neuron in the CA3 region of rodent hippocampus. Mol Cell Neurosci. 2003;24:709-24 pubmed..Kir3.3 neurons might modulate the activity of CA3 circuitries and consequently memory processing in the hippocampus. ..
- Weger S, Hammer E, Götz A, Heilbronn R. Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2). Virology. 2007;362:192-206 pubmed..Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment. ..
- Mössinger J, Wieffer M, Krause E, Freund C, Gerth F, Krauss M, et al. Phosphatidylinositol 4-kinase II? function at endosomes is regulated by the ubiquitin ligase Itch. EMBO Rep. 2012;13:1087-94 pubmed publisher..These findings unravel a new molecular link between phosphoinositide-regulated endosomal membrane traffic, ubiquitin and the modulation of Wnt signalling. ..
- Alex M, Weger S, Mietzsch M, Slanina H, Cathomen T, Heilbronn R. DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8. J Virol. 2012;86:2859-63 pubmed publisher..Our data suggest that HSV-dependent AAV replication is initiated by Rep contacting the AAV ITR and by cooperative binding of ICP8 on AAV ssDNA. ..
- Baldinger T, Gossen M. Binding of Drosophila ORC proteins to anaphase chromosomes requires cessation of mitotic cyclin-dependent kinase activity. Mol Cell Biol. 2009;29:140-9 pubmed publisher..Expression of either stable cyclin A, B, or B3 prevents this reassociation, suggesting that cessation of mitotic cyclin-dependent kinase activity is essential for binding of the DmOrc proteins to chromosomes. ..
- Maritzen T, Podufall J, Haucke V. Stonins--specialized adaptors for synaptic vesicle recycling and beyond?. Traffic. 2010;11:8-15 pubmed publisher..In this review, we summarize the current state of knowledge about mammalian stonins with a special focus on the role of stonin 2 in SV recycling at presynaptic nerve terminals. ..
- Aranda Orgilles B, Aigner J, Kunath M, Lurz R, Schneider R, Schweiger S. Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A. PLoS ONE. 2008;3:e3507 pubmed publisher..Furthermore, a failure in the microtubule directed transport of this protein complex would be an attractive mechanism underlying the pathogenesis of OS in patients with B-box1 mutations. ..
- Welzel F, Kaehler C, Isau M, Hallen L, Lehrach H, Krobitsch S. FOX-2 dependent splicing of ataxin-2 transcript is affected by ataxin-1 overexpression. PLoS ONE. 2012;7:e37985 pubmed publisher..Thus, our results demonstrate that FOX-2 is involved in splicing of ataxin-2 transcripts and that this splicing event is altered by overexpression of ataxin-1. ..
- Said A, Azab W, Damiani A, Osterrieder N. Equine herpesvirus type 4 UL56 and UL49.5 proteins downregulate cell surface major histocompatibility complex class I expression independently of each other. J Virol. 2012;86:8059-71 pubmed publisher..5 interferes with MHC-I antigen presentation by blocking peptide transport in the ER...
- Li P, Li J, Müller E, Otto A, Dietz R, von Harsdorf R. Phosphorylation by protein kinase CK2: a signaling switch for the caspase-inhibiting protein ARC. Mol Cell. 2002;10:247-58 pubmed..ARC is able to bind to caspase-8 only when it is localized to mitochondria but not to the cytoplasm. Our results reveal a molecular mechanism by which a caspase-inhibiting protein requires phosphorylation in order to prevent apoptosis. ..
- Theilig F, Goranova I, Hirsch J, Wieske M, Unsal S, Bachmann S, et al. Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney. Cell Physiol Biochem. 2008;21:63-74 pubmed publisher..Overall, the glomerulus, thin limbs and medullary collecting ducts were devoid of THIK-1 and THIK-2 signal. In summary, THIK-1 and THIK-2 are abundantly expressed in the proximal and distal nephron of the mammalian kidney. ..
- Dorner A, Giessen S, Gaub R, Grosse Siestrup H, Schwimmbeck P, Hetzer R, et al. An isoform shift in the cardiac adenine nucleotide translocase expression alters the kinetic properties of the carrier in dilated cardiomyopathy. Eur J Heart Fail. 2006;8:81-9 pubmed..90.4 +/- 2.9 microM in controls (p < 0.03). The altered isoform expression in DCM hearts entails changes in the kinetic properties of total ANT protein restricting ANT function and contributing to disturbed energy metabolism in DCM. ..
- Sinzelle L, Kapitonov V, Grzela D, Jursch T, Jurka J, Izsvák Z, et al. Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Proc Natl Acad Sci U S A. 2008;105:4715-20 pubmed publisher..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes. ..
- Jerke U, Rolle S, Purfürst B, Luft F, Nauseef W, Kettritz R. ?2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells. J Biol Chem. 2013;288:12910-9 pubmed publisher..The data suggest an alternative to EC uptake of soluble MPO, namely the cell contact-dependent, ?2 integrin-mediated transfer from neutrophils. The findings could be of therapeutic relevance in atherosclerosis and vasculitis. ..
- Leisle L, Ludwig C, Wagner F, Jentsch T, Stauber T. ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. EMBO J. 2011;30:2140-52 pubmed publisher..Our work suggests that gating underlies the rectification of all endosomal/lysosomal CLCs and extends the concept of voltage gating beyond channels to ion exchangers. ..
- Breiderhoff T, Christiansen G, Pallesen L, Vaegter C, Nykjaer A, Holm M, et al. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction. PLoS ONE. 2013;8:e75006 pubmed publisher..In conclusion, our studies have identified a novel function for VPS10P domain receptors in control of synaptic depression and suggest SORCS3 as a novel factor modulating aversive memory extinction. ..
- Wilhelm B, Mandad S, Truckenbrodt S, Kr hnert K, Sch fer C, Rammner B, et al. Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science. 2014;344:1023-8 pubmed publisher..In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones...
- Keil C, Gröbe T, Oei S. MNNG-induced cell death is controlled by interactions between PARP-1, poly(ADP-ribose) glycohydrolase, and XRCC1. J Biol Chem. 2006;281:34394-405 pubmed..In XRCC1-deficient cells, the same MNNG treatment caused non-apoptotic cell death without accumulation of poly(ADP-ribose). Thus, XRCC1 seems to be involved in regulating a poly(ADP-ribose)-mediated apoptotic cell death. ..
- Pech M, Karim Z, Yamamoto H, Kitakawa M, Qin Y, Nierhaus K. Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations. Proc Natl Acad Sci U S A. 2011;108:3199-203 pubmed publisher..The strong selective advantage conferred by the presence of EF4 at high intracellular ionic strength or low temperatures explains the ubiquitous distribution and high conservation of EF4. ..
- Brembeck F, Schwarz Romond T, Bakkers J, Wilhelm S, Hammerschmidt M, Birchmeier W. Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions. Genes Dev. 2004;18:2225-30 pubmed..During zebrafish embryogenesis, BCL9-2 acts in the Wnt8-signaling pathway and regulates mesoderm patterning. ..
- Bulwin G, Heinemann T, Bugge V, Winter M, Lohan A, Schlawinsky M, et al. TIRC7 inhibits T cell proliferation by modulation of CTLA-4 expression. J Immunol. 2006;177:6833-41 pubmed..Splenocytes obtained from CTLA-4-deficient mice are not responsive to TIRC7 Ab targeting. Thus, TIRC7 acts as an upstream regulatory molecule of CTLA-4 expression. ..
- Thaa B, Siche S, Herrmann A, Veit M. Acylation and cholesterol binding are not required for targeting of influenza A virus M2 protein to the hemagglutinin-defined budozone. FEBS Lett. 2014;588:1031-6 pubmed publisher..The motifs are thus irrelevant for M2 targeting in cells. ..
- Kononenko N, Diril M, Puchkov D, Kintscher M, Koo S, Pfuhl G, et al. Compromised fidelity of endocytic synaptic vesicle protein sorting in the absence of stonin 2. Proc Natl Acad Sci U S A. 2013;110:E526-35 pubmed publisher..Our results are consistent with a model whereby Stn2 is required to preserve SV protein composition but is dispensable for maintaining the speed of SV recycling. ..
- Willnow T, Andersen O. Sorting receptor SORLA--a trafficking path to avoid Alzheimer disease. J Cell Sci. 2013;126:2751-60 pubmed publisher..As discussed here, these findings uncovered a unique regulatory pathway for the control of neuronal protein transport, and provide clues as to why defects in this pathway cause neurodegenerative disease. ..
- Reiche J, Theilig F, Rafiqi F, Carlo A, Militz D, Mutig K, et al. SORLA/SORL1 functionally interacts with SPAK to control renal activation of Na(+)-K(+)-Cl(-) cotransporter 2. Mol Cell Biol. 2010;30:3027-37 pubmed publisher..Our data suggest a novel regulatory pathway whereby intracellular trafficking of SPAK by the sorting receptor SORLA is crucial for proper NKCC2 activation and for maintenance of renal ion balance. ..
- Andersen O, Schmidt V, Spoelgen R, Gliemann J, Behlke J, Galatis D, et al. Molecular dissection of the interaction between amyloid precursor protein and its neuronal trafficking receptor SorLA/LR11. Biochemistry. 2006;45:2618-28 pubmed..These data shed new light on the molecular determinants of neuronal APP trafficking and processing and on possible targets for intervention with senile plaque formation in patients with AD. ..
- Kaiser F, Kaufmann S, Zerrahn J. IIGP, a member of the IFN inducible and microbial defense mediating 47 kDa GTPase family, interacts with the microtubule binding protein hook3. J Cell Sci. 2004;117:1747-56 pubmed..Our experiments identify hook3 as the first cooperation partner of a member of the 47 kDa GTPase protein family and indicate that hook3 links in an IFNgamma-inducible fashion to cytoskeleton-based membrane trafficking. ..
- Wolff T, Unterstab G, Heins G, Richt J, Kann M. Characterization of an unusual importin alpha binding motif in the borna disease virus p10 protein that directs nuclear import. J Biol Chem. 2002;277:12151-7 pubmed
- Bothe M, Mundhenk L, Kaup M, Weise C, Gruber A. The murine goblet cell protein mCLCA3 is a zinc-dependent metalloprotease with autoproteolytic activity. Mol Cells. 2011;32:535-41 pubmed publisher..The data strongly suggest that secreted CLCA proteins have zinc-dependent autoproteolytic activity and that they may cleave additional proteins. ..
- Fogeron M, Müller H, Schade S, Dreher F, Lehmann V, Kühnel A, et al. LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells. Nat Commun. 2013;4:1531 pubmed publisher..We propose that LGALS3BP suppresses assembly of centriolar substructures, and when depleted, causes accumulation of centriolar complexes comprising CPAP, acetylated tubulin and centrin...
- Burgert T, Schmidt V, Caglayan S, Lin F, Füchtbauer A, Füchtbauer E, et al. SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes. Mol Cell Biol. 2013;33:4308-20 pubmed publisher..Thus, our data identified the importance of PACS1-dependent protein sorting for amyloidogenic-burden control via both SORLA-dependent and SORLA-independent mechanisms. ..
- Kolisek M, Nestler A, Vormann J, Schweigel Röntgen M. Human gene SLC41A1 encodes for the Na+/Mg²+ exchanger. Am J Physiol Cell Physiol. 2012;302:C318-26 pubmed publisher..Based on this finding, the analysis of Na(+)/Mg(2+) exchanger regulation and its involvement in the pathogenesis of diseases such as Parkinson's disease and hypertension at the molecular level should now be possible. ..
- Kozjak Pavlovic V, Ross K, Götz M, Goosmann C, Rudel T. A tag at the carboxy terminus prevents membrane integration of VDAC1 in mammalian mitochondria. J Mol Biol. 2010;397:219-32 pubmed publisher..This effect offers easy tools for studying the requirements for the membrane assembly of beta-barrel proteins, but also advises caution when interpreting the outcome of the beta-barrel protein overexpression experiments. ..
- Werner A, Sparkes I, Romeis T, Witte C. Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean. Plant Physiol. 2008;146:418-30 pubmed..These findings demonstrate that after the generation of allantoin in the peroxisome, plant purine degradation continues in the endoplasmic reticulum. ..
- Diril M, Schmidt S, Krauss M, Gawlik V, Joost H, Schurmann A, et al. Lysosomal localization of GLUT8 in the testis--the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction. FEBS J. 2009;276:3729-43 pubmed publisher..Therefore, we suggest a potential role for GLUT8 in the so far unexplored substrate transport across intracellular membranes. ..
- Ingmundson A, Nahar C, Brinkmann V, Lehmann M, Matuschewski K. The exported Plasmodium berghei protein IBIS1 delineates membranous structures in infected red blood cells. Mol Microbiol. 2012;83:1229-43 pubmed publisher..berghei-induced structures (IBIS) are membranous indicating that P. berghei, like P. falciparum, creates an intracellular membranous network in infected red blood cells...
- Henn V, Edemir B, Stefan E, Wiesner B, Lorenz D, Theilig F, et al. Identification of a novel A-kinase anchoring protein 18 isoform and evidence for its role in the vasopressin-induced aquaporin-2 shuttle in renal principal cells. J Biol Chem. 2004;279:26654-65 pubmed..AVP not only recruited AQP2, but also AKAP18delta to the plasma membrane. The elevation of cAMP caused the dissociation of AKAP18delta and PKA. The data suggest that AKAP18delta is involved in the AQP2 shuttle. ..
- Halic M, Gartmann M, Schlenker O, Mielke T, Pool M, Sinning I, et al. Signal recognition particle receptor exposes the ribosomal translocon binding site. Science. 2006;312:745-7 pubmed
- Nedvetsky P, Stefan E, Frische S, Santamaria K, Wiesner B, Valenti G, et al. A Role of myosin Vb and Rab11-FIP2 in the aquaporin-2 shuttle. Traffic. 2007;8:110-23 pubmed..In summary, we identified myosin Vb as a motor protein involved in AQP2 recycling and show that myosin Vb- and Rab11-FIP2-dependent recycling of AQP2 is an integral part of the AQP2 shuttle. ..
- Berger F, Lau C, Ziegler M. Regulation of poly(ADP-ribose) polymerase 1 activity by the phosphorylation state of the nuclear NAD biosynthetic enzyme NMN adenylyl transferase 1. Proc Natl Acad Sci U S A. 2007;104:3765-70 pubmed..We conclude that, depending on its state of phosphorylation, NMNAT-1 binds to activated, automodifying PARP-1 and thereby amplifies poly(ADP-ribosyl)ation. ..
- Eitel J, Krüll M, Hocke A, N Guessan P, Zahlten J, Schmeck B, et al. Beta-PIX and Rac1 GTPase mediate trafficking and negative regulation of NOD2. J Immunol. 2008;181:2664-71 pubmed..Overall, our data indicate that beta-PIX and Rac1 mediate trafficking and negative regulation of NOD2-dependent signaling which is different from Rac1's positive regulatory role in TLR2 signaling. ..
- Fouquet W, Owald D, Wichmann C, Mertel S, Depner H, Dyba M, et al. Maturation of active zone assembly by Drosophila Bruchpilot. J Cell Biol. 2009;186:129-45 pubmed publisher..BRP seems responsible for effectively clustering Ca(2+) channels beneath the T-bar density late in a protracted AZ formation process, potentially through a direct molecular interaction with intracellular Ca(2+) channel domains. ..
- Tavraz N, Dürr K, Koenderink J, Freilinger T, Bamberg E, Dichgans M, et al. Impaired plasma membrane targeting or protein stability by certain ATP1A2 mutations identified in sporadic or familial hemiplegic migraine. Channels (Austin). 2009;3:82-7 pubmed
- Kastner C, Pohl M, Sendeski M, Stange G, Wagner C, Jensen B, et al. Effects of receptor-mediated endocytosis and tubular protein composition on volume retention in experimental glomerulonephritis. Am J Physiol Renal Physiol. 2009;296:F902-11 pubmed publisher..Enhanced proteolytic cleavage of ENaC points to a novel mechanism of channel activation which may involve the action of filtered plasma proteases. ..
- Said A, Osterrieder N. Equine herpesvirus type 1 (EHV-1) open reading frame 59 encodes an early protein that is localized to the cytosol and required for efficient virus growth. Virology. 2014;449:263-9 pubmed publisher..Recombinant EHV-1 lacking ORF59 (rAb4?59) exhibited a small-plaque phenotype and could not be propagated. Our findings suggest that the ORF59 protein plays a major role in EHV-1 replication in vitro and likely in vivo. ..
- Alken M, Rutz C, Köchl R, Donalies U, Oueslati M, Furkert J, et al. The signal peptide of the rat corticotropin-releasing factor receptor 1 promotes receptor expression but is not essential for establishing a functional receptor. Biochem J. 2005;390:455-64 pubmed..Thus the signal peptide of the CRF-R1 promotes an early step of receptor biogenesis, such as targeting of the nascent chain to the ER membrane and/or the gating of the protein-conducting translocon of the ER membrane. ..
- Knoll M, Yanagisawa Y, Simmons S, Engels N, Wienands J, Melchers F, et al. The non-Ig parts of the VpreB and ?5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor. J Immunol. 2012;188:6010-7 pubmed publisher..By contrast, the non-Ig part of ?5 crosslinks preBCRs for downregulation and stimulation. ..
- Köchl R, Alken M, Rutz C, Krause G, Oksche A, Rosenthal W, et al. The signal peptide of the G protein-coupled human endothelin B receptor is necessary for translocation of the N-terminal tail across the endoplasmic reticulum membrane. J Biol Chem. 2002;277:16131-8 pubmed..We postulate that signal peptides are necessary for those G protein-coupled receptors for which post-translational translocation of the N terminus is impaired or blocked by the presence of stably folded domains. ..
- Delroisse J, Ullrich Lüter E, Blaue S, Ortega Martínez O, Eeckhaut I, Flammang P, et al. A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence. Open Biol. 2017;7: pubmed publisher..In these two benthic suspension-feeding species, similar ecological pressures would constitute strong selective forces for the functional shift of these enzymes and the emergence of bioluminescence. ..
- Zhao C, Anand R, Braunewell K. Nicotine-induced Ca2+-myristoyl switch of neuronal Ca2+ sensor VILIP-1 in hippocampal neurons: a possible crosstalk mechanism for nicotinic receptors. Cell Mol Neurobiol. 2009;29:273-86 pubmed publisher..VILIP-1 might provide a novel functional crosstalk between alpha4- and alpha7-containing nAChRs. ..
- Zander J, Münster Wandowski A, Brunk I, Pahner I, Gómez Lira G, Heinemann U, et al. Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses. J Neurosci. 2010;30:7634-45 pubmed publisher..Thus, synaptic and vesicular coexistence of VGLUT and VGAT is more widespread than previously anticipated, putatively influencing fine-tuning and control of synaptic plasticity. ..
- Kaufer B, Smejkal B, Osterrieder N. The varicella-zoster virus ORFS/L (ORF0) gene is required for efficient viral replication and contains an element involved in DNA cleavage. J Virol. 2010;84:11661-9 pubmed publisher..Since the sequence element has no counterpart at the other end of U(L), we concluded that our results can provide an explanation for the almost exclusive orientation of the U(L) seen in packaged VZV DNA...
- Chlench S, Mecha Disassa N, Hohberg M, Hoffmann C, Pohlkamp T, Beyer G, et al. Regulation of Foxo-1 and the angiopoietin-2/Tie2 system by shear stress. FEBS Lett. 2007;581:673-80 pubmed..eNOS and Tie2 were upregulated. No effects on Ang-1 expression were detected. In conclusion, Foxo-1 and Ang-2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis. ..
- Marinc C, Prüss H, Derst C, Veh R. Immunocytochemical localization of TASK-3 channels in rat motor neurons. Cell Mol Neurobiol. 2012;32:309-18 pubmed publisher..In situ hybridization using a TASK-3 specific riboprobe verified the expression of TASK-3 in motor neurons at the mRNA level. ..
- Lenk U, Sommer T. Ubiquitin-mediated proteolysis of a short-lived regulatory protein depends on its cellular localization. J Biol Chem. 2000;275:39403-10 pubmed..These observations imply that both the cellular targeting of a substrate and the compartment-specific activity of components of the ubiquitin-proteasome system define the half-life of naturally short-lived proteins. ..
- Mitter D, Reisinger C, Hinz B, Hollmann S, Yelamanchili S, Treiber Held S, et al. The synaptophysin/synaptobrevin interaction critically depends on the cholesterol content. J Neurochem. 2003;84:35-42 pubmed..Variations in the availability of cholesterol may promote or impair synaptic efficiency by interfering with this complex. ..