Experts and Doctors on peptide hydrolases in Berlin, Germany


Locale: Berlin, Germany
Topic: peptide hydrolases

Top Publications

  1. Braumann C, Tangermann J, Jacobi C, Muller J, Dubiel W. Novel anti-angiogenic compounds for application in tumor therapy - COP9 signalosome-associated kinases as possible targets. Mini Rev Med Chem. 2008;8:421-8 pubmed
    ..Whereas curcumin and emodin can act via inhibition of COP9 signalosome-associated kinases, taurolidine blocks protein biosynthesis. ..
  2. Siems W, Maul B, Wiesner B, Becker M, Walther T, Rothe L, et al. Effects of kinins on mammalian spermatozoa and the impact of peptidolytic enzymes. Andrologia. 2003;35:44-54 pubmed
    ..The effects of Bk on sperm cells are not mediated by the B2 Bk receptor. Using several biochemical, molecular and genetic methods we could not detect any Bk receptor on spermatozoa. ..
  3. Gummlich L, Rabien A, Jung K, Dubiel W. Deregulation of the COP9 signalosome-cullin-RING ubiquitin-ligase pathway: mechanisms and roles in urological cancers. Int J Biochem Cell Biol. 2013;45:1327-37 pubmed publisher
    ..Recently evaluated miRNAs altered in urological cancers might have impact on the CSN-CRL pathway which has to be analyzed in future experiments. We propose that the pathway is a suitable target for future tumor therapy. ..
  4. Gummlich L, Kähne T, Naumann M, Kilic E, Jung K, Dubiel W. New Insights Into the Mechanism of COP9 Signalosome-Cullin-RING Ubiquitin-Ligase Pathway Deregulation in Urological Cancers. Int Rev Cell Mol Biol. 2016;323:181-229 pubmed publisher
    ..The occurrence of p27(Kip1)V109G and p27(Kip1)I119T in RCC makes the CSN-CRL pathway an attractive therapeutic target. ..
  5. Richter R, Hejazi M, Kraft R, Ziegler K, Lockau W. Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterizatio. Eur J Biochem. 1999;263:163-9 pubmed
    ..Based on inhibitor sensitivity and primary sequence, cyanophycinase appears to be a serine-type exopeptidase related to dipeptidase E [Conlin, C.A., Haakensson, K., Liljas, A. & Miller, C.G. (1994) J. Bacteriol. 176, 166-172]. ..
  6. Bech Otschir D, Kraft R, Huang X, Henklein P, Kapelari B, Pollmann C, et al. COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system. EMBO J. 2001;20:1630-9 pubmed
    ..It induces the cyclin-dependent inhibitor p21. In HeLa and MCF-7 cells, inhibition of CSN kinase by curcumin or Deltap53(145-164) results in accumulation of endogenous p53. ..
  7. George M, Schwecke T, Beimforde N, Hohn O, Chudak C, Zimmermann A, et al. Identification of the protease cleavage sites in a reconstituted Gag polyprotein of an HERV-K(HML-2) element. Retrovirology. 2011;8:30 pubmed publisher
    ..During this process, the subdomains of the Gag polyproteins are released by proteolytic cleavage, although the nature of the mature HERV-K(HML-2) Gag proteins and the exact position of the cleavage sites have until now remained unknown...
  8. Hannss R, Dubiel W. COP9 signalosome function in the DDR. FEBS Lett. 2011;585:2845-52 pubmed publisher
    ..DDR is a protection against cancer and deregulation of CSN function causes cancer making it an attractive pharmacological target. Here we review current knowledge on CSN function in DDR. ..
  9. Peth A, Boettcher J, Dubiel W. Ubiquitin-dependent proteolysis of the microtubule end-binding protein 1, EB1, is controlled by the COP9 signalosome: possible consequences for microtubule filament stability. J Mol Biol. 2007;368:550-63 pubmed
    ..A nocodazole-induced cell-cycle arrest was partially rescued by CSN1 or EB1. These data demonstrate that the CSN-dependent protection of EB1 is important for microtubule function. ..

More Information


  1. Naumann M, Bech Otschir D, Huang X, Ferrell K, Dubiel W. COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK. J Biol Chem. 1999;274:35297-300 pubmed
    ..The c-Jun activation caused by Sgn2 overexpression is independent of JNK and mitogen-activated protein kinase kinase 4. The data demonstrate the existence of a novel COP9 signalosome-directed c-Jun activation pathway. ..
  2. Dechend R, Hirano F, Lehmann K, Heissmeyer V, Ansieau S, Wulczyn F, et al. The Bcl-3 oncoprotein acts as a bridging factor between NF-kappaB/Rel and nuclear co-regulators. Oncogene. 1999;18:3316-23 pubmed
    ..These data implicate Bcl-3 as an adaptor between NF-kappaB p50/p52 and other transcription regulators and suggest that its gene activation function may at least in part be due to recruitment of the Tip60 histone actetylase. ..
  3. Seeger M, Kraft R, Ferrell K, Bech Otschir D, Dumdey R, Schade R, et al. A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits. FASEB J. 1998;12:469-78 pubmed
    ..Considering the putative role of the complex in signal transduction and its widespread occurrence, we suggest the name JAB1-containing signalosome. ..
  4. Dubiel W, Ferrell K, Dumdey R, Standera S, Prehn S, Rechsteiner M. Molecular cloning and expression of subunit 12: a non-MCP and non-ATPase subunit of the 26 S protease. FEBS Lett. 1995;363:97-100 pubmed
    ..In contrast, after transfer from native gels, the epitope(s) recognized by anti-S12f205 is exposed in the regulatory complex but appears to be masked when the regulatory complex associates with the multicatalytic protease. ..
  5. Kukhtina V, Kottwitz D, Strauss H, Heise B, Chebotareva N, Tsetlin V, et al. Intracellular domain of nicotinic acetylcholine receptor: the importance of being unfolded. J Neurochem. 2006;97 Suppl 1:63-7 pubmed
    ..Identification of proteins that interact with the intracellular domain will provide a better understanding of protein-protein interactions involved in nAChR assembly, trafficking and clustering...
  6. Artuc M, Bohm M, Grützkau A, Smorodchenko A, Zuberbier T, Luger T, et al. Human mast cells in the neurohormonal network: expression of POMC, detection of precursor proteases, and evidence for IgE-dependent secretion of alpha-MSH. J Invest Dermatol. 2006;126:1976-81 pubmed
    ..Moreover, IgE-dependent release of alpha-MSH suggests an immunomodulatory role of this neurohormone during inflammatory and allergic reactions of the skin. ..
  7. Hetfeld B, Peth A, Sun X, Henklein P, Cohen G, Dubiel W. The COP9 signalosome-mediated deneddylation is stimulated by caspases during apoptosis. Apoptosis. 2008;13:187-95 pubmed
    ..The cleavage of Rbx1 and increased deneddylation of cullins inactivate CRLs and presumably stabilize pro-apoptotic factors for final apoptotic steps. ..
  8. Lang C, Bottner M, Holz C, Veen M, Ryser M, Reindl A, et al. Specific Lactobacillus/Mutans Streptococcus co-aggregation. J Dent Res. 2010;89:175-9 pubmed publisher
    ..It is thus seen to present a potential strategy for in vivo alteration of plaque biofilm and caries. ..
  9. Urban S, Textoris Taube K, Reimann B, Janek K, Dannenberg T, Ebstein F, et al. The efficiency of human cytomegalovirus pp65(495-503) CD8+ T cell epitope generation is determined by the balanced activities of cytosolic and endoplasmic reticulum-resident peptidases. J Immunol. 2012;189:529-38 pubmed publisher
    ..As a consequence, our experiments reveal that the combination of cytosolic and ER-resident peptidase activities strongly shape the pool of specific antigenic peptides and thus modulate MHC class I epitope presentation efficiency. ..
  10. Schlosser A, Vanselow J, Kramer A. Mapping of phosphorylation sites by a multi-protease approach with specific phosphopeptide enrichment and NanoLC-MS/MS analysis. Anal Chem. 2005;77:5243-50 pubmed
  11. Hetfeld B, Helfrich A, Kapelari B, Scheel H, Hofmann K, Guterman A, et al. The zinc finger of the CSN-associated deubiquitinating enzyme USP15 is essential to rescue the E3 ligase Rbx1. Curr Biol. 2005;15:1217-21 pubmed
    ..In summary, a functional Zn finger of USP15 is needed to maintain a conformation essential for disassembling poly-Ub chains, a prerequisite for rescuing the E3 ligase Rbx1. ..
  12. Eitinger T. In vivo production of active nickel superoxide dismutase from Prochlorococcus marinus MIT9313 is dependent on its cognate peptidase. J Bacteriol. 2004;186:7821-5 pubmed
    ..NiSOD activity was observed in Escherichia coli when sodN and sodX (encoding a putative peptidase) from Prochlorococcus marinus MIT9313 were coexpressed...
  13. Jarosch E, Taxis C, Volkwein C, Bordallo J, Finley D, Wolf D, et al. Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48. Nat Cell Biol. 2002;4:134-9 pubmed
    ..Moreover, our results suggest that the AAA proteins of the 26S proteasome are not directly involved in substrate export. Instead, a related AAA complex Cdc48, is required for ER-associated protein degradation upstream of the proteasome. ..
  14. Dubiel W, Ferrell K, Rechsteiner M. Tat-binding protein 7 is a subunit of the 26S protease. Biol Chem Hoppe Seyler. 1994;375:237-40 pubmed
    ..Based on peptide matches covering more than 10% of the TBP7 sequence, and the fact that the migration of S6 on SDS-PAGE is consistent with the estimated molecular mass for TBP7, we conclude that subunit 6 of the 26S protease is TBP7. ..
  15. Ziemert N, Ishida K, Liaimer A, Hertweck C, Dittmann E. Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria. Angew Chem Int Ed Engl. 2008;47:7756-9 pubmed publisher
  16. Lenk U, Yu H, Walter J, Gelman M, Hartmann E, Kopito R, et al. A role for mammalian Ubc6 homologues in ER-associated protein degradation. J Cell Sci. 2002;115:3007-14 pubmed
    ..Similarly, we describe that the expression level of Ubc6p in yeast is also critical for ERAD, suggesting that the Ubc6p function is highly conserved from yeast to mammals. ..