Genomes and Genes
Experts and Doctors on mitosis in Heidelberg, Baden Württemberg, Germany
Locale: Heidelberg, Baden Württemberg, Germany
- De Simone A, NÃ©dÃ©lec F, GÃ¶nczy P. Dynein Transmits Polarized Actomyosin Cortical Flows to Promote Centrosome Separation. Cell Rep. 2016;14:2250-2262 pubmed publisher..This mechanism elegantly couples cell polarization with centrosome separation, thus ensuring faithful cell division. ..
- Caydasi A, Lohel M, Grünert G, Dittrich P, Pereira G, Ibrahim B. A dynamical model of the spindle position checkpoint. Mol Syst Biol. 2012;8:582 pubmed publisher..Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition. ..
- Haering C, Jessberger R. Cohesin in determining chromosome architecture. Exp Cell Res. 2012;318:1386-93 pubmed publisher..This review focuses on cohesin's role in structuring chromosomes during mitotic and meiotic cell divisions and during interphase. ..
- Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, et al. Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells. Oncotarget. 2016;7:4062-76 pubmed publisher..This study suggests that the APC/C and its co-activator Cdc20 could be a new and promising target especially in high-risk MM patients. ..
- Meitinger F, Petrova B, Lombardi I, Bertazzi D, Hub B, Zentgraf H, et al. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010;123:1851-61 pubmed publisher..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
- Edgar B, Zielke N, Gutierrez C. Endocycles: a recurrent evolutionary innovation for post-mitotic cell growth. Nat Rev Mol Cell Biol. 2014;15:197-210 pubmed publisher..Endocycles probably evolved many times, and the various endocycle mechanisms found in nature highlight the versatility of the cell cycle control machinery. ..
- Nilsson I, Hoffmann I. Cell cycle regulation by the Cdc25 phosphatase family. Prog Cell Cycle Res. 2000;4:107-14 pubmed..Cdc25A and B are potential human oncogenes. In addition, Cdc25 is a main player of the G2 arrest caused by DNA damage or in the presence of unreplicated DNA. ..
- Meitinger F, Boehm M, Hofmann A, Hub B, Zentgraf H, Lehmann W, et al. Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes Dev. 2011;25:875-88 pubmed publisher..Genetic and functional data support a model in which Dbf2-Mob1 regulates Hof1 by inducing domain rearrangements, leading to the exposure of the Hof1 RLS domain during telophase. ..
- Gruss O, Wittmann M, Yokoyama H, Pepperkok R, Kufer T, Sillje H, et al. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nat Cell Biol. 2002;4:871-9 pubmed..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells. ..
- Andersen S, Ashford A, Tournebize R, Gavet O, Sobel A, Hyman A, et al. Mitotic chromatin regulates phosphorylation of Stathmin/Op18. Nature. 1997;389:640-3 pubmed..We have also shown that Stathmin/Op18 is one of the factors regulated by mitotic chromatin that governs preferential microtubule growth around chromosomes during spindle assembly. ..
- Kammerer D, Stevermann L, Liakopoulos D. Ubiquitylation regulates interactions of astral microtubules with the cleavage apparatus. Curr Biol. 2010;20:1233-43 pubmed publisher..Photoconversion experiments showed that Kar9 association with aMTs is stable. We propose that ubiquitylation controls interactions of aMTs with the cleavage apparatus through localized disassembly of Kar9 complexes. ..
- Gorjanacz M, Klerkx E, Galy V, Santarella R, Lopez Iglesias C, Askjaer P, et al. Caenorhabditis elegans BAF-1 and its kinase VRK-1 participate directly in post-mitotic nuclear envelope assembly. EMBO J. 2007;26:132-43 pubmed..We propose that phosphorylation of BAF by VRK plays an essential regulatory role in the association of BAF with chromatin and nuclear membrane proteins during NE formation. ..
- Zigman M, Laumann Lipp N, Titus T, Postlethwait J, Moens C. Hoxb1b controls oriented cell division, cell shape and microtubule dynamics in neural tube morphogenesis. Development. 2014;141:639-49 pubmed publisher..We propose that Hox genes can influence global tissue morphogenesis by control of microtubule dynamics in individual cells in vivo. ..
- Cizmecioglu O, Warnke S, Arnold M, Duensing S, Hoffmann I. Plk2 regulated centriole duplication is dependent on its localization to the centrioles and a functional polo-box domain. Cell Cycle. 2008;7:3548-55 pubmed..In addition, we find that siRNA-mediated downregulation of Plk2 leads to the formation of abnormal mitotic spindles confirming that Plk2 may have a function in the reproduction of centrioles. ..
- Fumagalli S, Totty N, Hsuan J, Courtneidge S. A target for Src in mitosis. Nature. 1994;368:871-4 pubmed..Purification and microsequencing of p68 showed that it was related to the previously described GAP-associated protein p62 (ref. 6). ..
- Mata J, Curado S, Ephrussi A, Rørth P. Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis. Cell. 2000;101:511-22 pubmed..By regulating CDC25, Tribbles serves to coordinate entry into mitosis with morphogenesis and cell fate determination. ..
- Tibelius A, Marhold J, Zentgraf H, Heilig C, Neitzel H, Ducommun B, et al. Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1. J Cell Biol. 2009;185:1149-57 pubmed publisher..In this study, we show that a lack of MCPH1 or PCNT results in a loss of Chk1 from centrosomes with subsequently deregulated activation of centrosomal cyclin B-Cdk1. ..
- Maier B, Kirsch M, Anderhub S, Zentgraf H, Krämer A. The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells. Cell Cycle. 2013;12:1457-71 pubmed publisher..Together, we propose that MISP links microtubules to the actin cytoskeleton and focal adhesions in order to properly position the mitotic spindle. ..
- Mardin B, Isokane M, Cosenza M, KrÃ¤mer A, Ellenberg J, Fry A, et al. EGF-induced centrosome separation promotes mitotic progression and cell survival. Dev Cell. 2013;25:229-40 pubmed publisher..This has important implications for cancer therapy because cells with high centrosomal response to EGF are more susceptible to combinatorial inhibition of EGFR and Eg5. ..
- Petrova B, Dehler S, Kruitwagen T, Hériché J, Miura K, Haering C. Quantitative analysis of chromosome condensation in fission yeast. Mol Cell Biol. 2013;33:984-98 pubmed publisher..Thus, the chromosome condensation assay provides a direct and sensitive system for the discovery and characterization of components of the chromosome condensation machinery in a genetically tractable eukaryote. ..
- Tegha Dunghu J, Neumann B, Reber S, Krause R, Erfle H, Walter T, et al. EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. J Cell Sci. 2008;121:1718-26 pubmed publisher..Our proteomic identification screen combined with sensitive phenotypic analysis therefore provides a reliable platform for the identification and characterisation of proteins important for correct cell division. ..
- Lankenau D, Gloor G. In vivo gap repair in Drosophila: a one-way street with many destinations. Bioessays. 1998;20:317-27 pubmed..This review summarizes results that provide new insights into the process of gap repair in Drosophila and outline some recent experiments that demonstrate the power of the gene targeting technique. ..
- Grigaravicius P, Kaminska E, HÃ¼bner C, McKinnon P, von Deimling A, Frappart P. Rint1 inactivation triggers genomic instability, ER stress and autophagy inhibition in the brain. Cell Death Differ. 2016;23:454-68 pubmed publisher..Altogether, our findings highlight the crucial roles of Rint1 in vivo in genomic stability maintenance, as well as in prevention of ER stress and autophagy. ..
- Telley I, Gaspar I, Ephrussi A, Surrey T. A single Drosophila embryo extract for the study of mitosis ex vivo. Nat Protoc. 2013;8:310-24 pubmed publisher..The assay enables a unique combination of genetic, biochemical, optical and mechanical manipulations of the mitotic machinery. ..
- Ferby I, Blazquez M, Palmer A, Eritja R, Nebreda A. A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes. Genes Dev. 1999;13:2177-89 pubmed..Our results identify a novel p34(cdc2) binding and activating protein that regulates the G(2)/M transition during oocyte maturation. ..
- Palani S, Meitinger F, Boehm M, Lehmann W, Pereira G. Cdc14-dependent dephosphorylation of Inn1 contributes to Inn1-Cyk3 complex formation. J Cell Sci. 2012;125:3091-6 pubmed publisher..We propose that Cdc14 counteracts Cdk1 phosphorylation of Inn1 to facilitate Inn1-Cyk3 complex formation and so promote cytokinesis. ..
- Isokane M, Walter T, Mahen R, Nijmeijer B, HÃ©richÃ© J, Miura K, et al. ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol. 2016;212:647-59 pubmed publisher..This mitosis-specific role is independent of ARHGEF17's RhoGEF activity in interphase. Our study thus assigns a new mitotic function to ARHGEF17 and reveals the molecular mechanism for a key step in SAC establishment. ..
- Schecher S, Walter B, Falkenstein M, Macher Goeppinger S, Stenzel P, KrÃ¼mpelmann K, et al. Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer. Int J Cancer. 2017;141:1643-1653 pubmed publisher..Moreover, our results indicate that Cyclin K is a putative predictive biomarker for clinical outcome and therapy response for patients with prostate cancer. ..
- Tournebize R, Popov A, Kinoshita K, Ashford A, Rybina S, Pozniakovsky A, et al. Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nat Cell Biol. 2000;2:13-9 pubmed..These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1. ..
- Pau G, Walter T, Neumann B, Hériché J, Ellenberg J, Huber W. Dynamical modelling of phenotypes in a genome-wide RNAi live-cell imaging assay. BMC Bioinformatics. 2013;14:308 pubmed publisher..The described population-based modelling method might be applicable to other large-scale cell-based assays with temporal readout when only population-level measures are available. ..
- Cuylen S, Haering C. Deciphering condensin action during chromosome segregation. Trends Cell Biol. 2011;21:552-9 pubmed publisher..In this review we discuss possible mechanisms that could explain the variety of condensin actions during chromosome segregation...
- Mardin B, Agircan F, Lange C, Schiebel E. Plk1 controls the Nek2A-PP1? antagonism in centrosome disjunction. Curr Biol. 2011;21:1145-51 pubmed publisher..We propose that centrosome disjunction is regulated by Plk1, providing a well-balanced control between the counteracting Nek2A and PP1? activities on the centrosome linker. ..
- Gawlinski P, Nikolay R, Goursot C, Lawo S, Chaurasia B, Herz H, et al. The Drosophila mitotic inhibitor Frühstart specifically binds to the hydrophobic patch of cyclins. EMBO Rep. 2007;8:490-6 pubmed..We propose that binding of Frs to cyclins blocks the hydrophobic patch to interfere with Cdk1 substrate recognition. ..
- Bischoff F, Ponstingl H. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature. 1991;354:80-2 pubmed..Here we show that RCC1 specifically catalyses the exchange of guanine nucleotides on the Ran protein but not on the protein c-Ha-ras p21 (p21ras). ..
- König C, Maekawa H, Schiebel E. Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol. 2010;188:351-68 pubmed publisher..Consistent with this model, only triple mutants that lack BUB2 and the Cdk1 phosphorylation sites in Mob1 and Cdc15 show mitotic exit defects. ..
- Jekely G. Small GTPases and the evolution of the eukaryotic cell. Bioessays. 2003;25:1129-38 pubmed..Based on the reconstruction of putative roles for ancestral small GTPases, a hypothetical scenario on the origins of the first endomembranes, the nucleus, and phagocytosis is presented. ..
- Stachora A, Schäfer R, Pohlmeier M, Maier G, Ponstingl H. Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis. FEBS Lett. 1997;409:74-8 pubmed..One of them, with the consensus sequence P-T/S-P-S-P-Q/A-S/G-Y, is similar to the C-terminal domain in the largest subunit of RNA polymerase II. ..
- Maier P, Rathfelder N, Finkbeiner M, Taxis C, Mazza M, Le Panse S, et al. Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. EMBO J. 2007;26:1843-52 pubmed
- Rebacz B, Larsen T, Clausen M, Rønnest M, Loffler H, Ho A, et al. Identification of griseofulvin as an inhibitor of centrosomal clustering in a phenotype-based screen. Cancer Res. 2007;67:6342-50 pubmed
- Carmo Fonseca M, Ferreira J, Lamond A. Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis--evidence that the coiled body is a kinetic nuclear structure. J Cell Biol. 1993;120:841-52 pubmed..CBs are thus, dynamic nuclear organelles and we propose that cycling interactions of splicing snRNPs with CBs may be important for their participation in the processing or transport of pre-mRNA in mammalian cells. ..
- Cuylen S, Blaukopf C, Politi A, MULLER REICHERT T, Neumann B, Poser I, et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature. 2016;535:308-12 pubmed publisher..Our study thus elucidates a biomechanical role of the mitotic chromosome periphery in mammalian cells and suggests that natural proteins can function as surfactants in intracellular compartmentalization. ..
- Askjaer P, Galy V, Hannak E, Mattaj I. Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos. Mol Biol Cell. 2002;13:4355-70 pubmed..Finally, we have demonstrated that both IMA-2 and IMB-1, the homologues of vertebrate importin alpha and beta, are essential for both spindle assembly and nuclear formation in early embryos. ..
- Gonzalez C, Sunkel C, Glover D. Interactions between mgr, asp, and polo: asp function modulated by polo and needed to maintain the poles of monopolar and bipolar spindles. Chromosoma. 1998;107:452-60 pubmed..We speculate that Asp protein might be a substrate for the serine-threonine protein kinase encoded by polo. ..
- Brendle A, Brandt A, Johansson R, Enquist K, Hallmans G, Hemminki K, et al. Single nucleotide polymorphisms in chromosomal instability genes and risk and clinical outcome of breast cancer: a Swedish prospective case-control study. Eur J Cancer. 2009;45:435-42 pubmed publisher..54-0.99). As there is increasing evidence that CENPF is associated with poor prognosis in patients with primary BC, further independent studies are needed to clarify the importance of genetic variation in the CENPF gene in the clinic. ..
- Lange B, Bachi A, Wilm M, Gonzalez C. Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates. EMBO J. 2000;19:1252-62 pubmed..This suggests that another role of Hsp90 might be to ensure proper centrosome function. ..
- Thole T, Lodrini M, Fabian J, Wuenschel J, Pfeil S, Hielscher T, et al. Neuroblastoma cells depend on HDAC11 for mitotic cell cycle progression and survival. Cell Death Dis. 2017;8:e2635 pubmed publisher..The data indicate a significant role of HDAC11 for mitotic cell cycle progression and survival of MYCN-amplified neuroblastoma cells, and suggests that HDAC11 could be a valuable drug target. ..
- B renz F, Inoue D, Yokoyama H, Tegha Dunghu J, Freiss S, Draeger S, et al. The centriolar satellite protein SSX2IP promotes centrosome maturation. J Cell Biol. 2013;202:81-95 pubmed publisher..It preserves centrosome integrity and faithful mitosis during the rapid cleavage division of blastomeres and in somatic cells...
- ..Here, we review the complex interplay between Wnt signalling and the cell cycle. ..
- Carazo Salas R, Guarguaglini G, Gruss O, Segref A, Karsenti E, Mattaj I. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature. 1999;400:178-81 pubmed..We propose that RCC1 generates a high local concentration of Ran-GTP around chromatin which in turn induces the local nucleation of microtubules. ..
- Galy V, Askjaer P, Franz C, Lopez Iglesias C, Mattaj I. MEL-28, a novel nuclear-envelope and kinetochore protein essential for zygotic nuclear-envelope assembly in C. elegans. Curr Biol. 2006;16:1748-56 pubmed..We show that MEL-28 is an early-assembling, stable NE component required for all aspects of NE assembly. ..
- Giarre M, Török I, Schmitt R, Gorjanacz M, Kiss I, Mechler B. Patterns of importin-alpha expression during Drosophila spermatogenesis. J Struct Biol. 2002;140:279-90 pubmed..These data indicate that each Imp-alpha protein carries during Drosophila spermatogenesis distinct, albeit overlapping, functions that may involve nuclear import of proteins, microtubule organisation, and other yet unknown processes. ..
- Torok I, Strand D, Schmitt R, Tick G, Torok T, Kiss I, et al. The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis. J Cell Biol. 1995;129:1473-89 pubmed..Moreover, the cell cycle-dependent accumulation of OHO31 in the nucleus indicates that this protein may be required for critical nuclear reactions occurring at the onset of mitosis. ..
- Bertazzi D, Kurtulmus B, Pereira G. The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4. J Cell Biol. 2011;193:1033-48 pubmed publisher..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB. ..
- Bayliss R, Sardon T, Vernos I, Conti E. Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. Mol Cell. 2003;12:851-62 pubmed..The recognition between Aurora-A and TPX2 resembles that between the cAPK catalytic core and its flanking regions, suggesting this molecular mechanism may be a recurring theme in kinase regulation. ..
- Schaefer M, Steringer J, Lyko F. The Drosophila cytosine-5 methyltransferase Dnmt2 is associated with the nuclear matrix and can access DNA during mitosis. PLoS ONE. 2008;3:e1414 pubmed publisher..Our results represent the first comprehensive characterization of Dnmt2 proteins on the cellular level and have important implications for our understanding of the molecular activities of Dnmt2. ..
- Asencio C, Davidson I, Santarella Mellwig R, Ly Hartig T, Mall M, Wallenfang M, et al. Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis. Cell. 2012;150:122-35 pubmed publisher..By coordinating VRK-1- and PP2A-mediated signaling on BAF, Lem4/LEM-4L controls postmitotic NE formation in a function conserved from worms to humans. ..
- Zhang Y, Heermann D. Loops determine the mechanical properties of mitotic chromosomes. PLoS ONE. 2011;6:e29225 pubmed publisher..We suggest that changes in the mechanical characteristics of chromosomes in different stages of the cell cycle, for example, can be explained by an altered internal loop structure...
- Martin C, Ahmad I, Klingseisen A, Hussain M, Bicknell L, Leitch A, et al. Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nat Genet. 2014;46:1283-1292 pubmed publisher..Furthermore, we establish that different levels of impaired PLK4 activity result in growth and cilia phenotypes, providing a mechanism by which microcephaly disorders can occur with or without ciliopathic features. ..
- Hofmann J, Tegha Dunghu J, Dräger S, Will C, Luhrmann R, Gruss O. The Prp19 complex directly functions in mitotic spindle assembly. PLoS ONE. 2013;8:e74851 pubmed publisher..Our data identify the Prp19 complex as the first spliceosome subcomplex that directly contributes to mitosis in vertebrates independently of its function in interphase. ..
- Yokoyama H, Rybina S, Santarella Mellwig R, Mattaj I, Karsenti E. ISWI is a RanGTP-dependent MAP required for chromosome segregation. J Cell Biol. 2009;187:813-29 pubmed publisher..Our results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle MTs in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation...