Experts and Doctors on membrane transport proteins in Freiburg, Baden Württemberg, Germany


Locale: Freiburg, Baden Württemberg, Germany
Topic: membrane transport proteins

Top Publications

  1. Fröbel J, Rose P, Muller M. Twin-arginine-dependent translocation of folded proteins. Philos Trans R Soc Lond B Biol Sci. 2012;367:1029-46 pubmed publisher
    ..Ultimately, this leads to the formation of a transmembrane protein conduit that possibly consists of a pore-like TatA structure. The translocation step again is dependent on the PMF. ..
  2. Kern W, Steinke P, Schumacher A, Schuster S, von Baum H, Bohnert J. Effect of 1-(1-naphthylmethyl)-piperazine, a novel putative efflux pump inhibitor, on antimicrobial drug susceptibility in clinical isolates of Escherichia coli. J Antimicrob Chemother. 2006;57:339-43 pubmed
    ..NMP is moderately active in reversing MDR in clinical isolates of E. coli and can partially restore fluoroquinolone susceptibility through inhibition of efflux pumps. ..
  3. Speck J, Arndt K, Müller K. Efficient phage display of intracellularly folded proteins mediated by the TAT pathway. Protein Eng Des Sel. 2011;24:473-84 pubmed publisher
  4. Deshmukh S, Kremer B, Freudenberg M, Bauer S, Golenbock D, Henneke P. Macrophages recognize streptococci through bacterial single-stranded RNA. EMBO Rep. 2011;12:71-6 pubmed publisher
    ..The role of ssRNA in the recognition of Gram-positive bacteria--leading to the induction of inflammatory cytokines--has potential implications for sepsis pathogenesis, diagnosis and treatment. ..
  5. Bohnert J, Schuster S, Kern W, Karcz T, Olejarz A, Kaczor A, et al. Novel Piperazine Arylideneimidazolones Inhibit the AcrAB-TolC Pump in Escherichia coli and Simultaneously Act as Fluorescent Membrane Probes in a Combined Real-Time Influx and Efflux Assay. Antimicrob Agents Chemother. 2016;60:1974-83 pubmed publisher
    ..The EPIs BM-38 and BM-19 displayed the most rapid influx of all compounds, whereas BM-27, which did not act as an EPI, showed the slowest influx. ..
  6. Bartholomé K, Rius M, Letschert K, Keller D, Timmer J, Keppler D. Data-based mathematical modeling of vectorial transport across double-transfected polarized cells. Drug Metab Dispos. 2007;35:1476-81 pubmed
    ..The same systems biology approach may be applied to other cellular systems and to different substances. ..
  7. Fröbel J, Rose P, Muller M. Early contacts between substrate proteins and TatA translocase component in twin-arginine translocation. J Biol Chem. 2011;286:43679-89 pubmed publisher
    ..It strictly depends on the presence of TatB and TatC, the latter of which is shown to make contacts with the transmembrane helix of TatA. ..
  8. Becker T, Wenz L, Thornton N, Stroud D, Meisinger C, Wiedemann N, et al. Biogenesis of mitochondria: dual role of Tom7 in modulating assembly of the preprotein translocase of the outer membrane. J Mol Biol. 2011;405:113-24 pubmed publisher
    ..Thus, Tom7 modulates the biogenesis of topologically different proteins, the ?-barrel forming protein Tom40 and Tom22 that contains a transmembrane ?-helix. ..
  9. Chacinska A, van der Laan M, Mehnert C, Guiard B, Mick D, Hutu D, et al. Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol Cell Biol. 2010;30:307-18 pubmed publisher
    ..Depending on the signals of the preproteins, switches between the different forms of supercomplex and TIM23 are required for the completion of preprotein import. ..

More Information


  1. Kutik S, Rissler M, Guan X, Guiard B, Shui G, Gebert N, et al. The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesis. J Cell Biol. 2008;183:1213-21 pubmed publisher
    ..The pleiotropic effects of the translocator maintenance protein on preprotein import and respiratory chain can be attributed to its role in biosynthesis of mitochondrial cardiolipin. ..
  2. Rieg S, Huth A, Kalbacher H, Kern W. Resistance against antimicrobial peptides is independent of Escherichia coli AcrAB, Pseudomonas aeruginosa MexAB and Staphylococcus aureus NorA efflux pumps. Int J Antimicrob Agents. 2009;33:174-6 pubmed publisher
    ..These findings do not support a critical role of MDR efflux pumps in the tested pathogens as a strategy to increase virulence by circumventing the antimicrobial action of innate defence AMPs. ..
  3. Panahandeh S, Maurer C, Moser M, DeLisa M, Muller M. Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli. J Biol Chem. 2008;283:33267-75 pubmed publisher
    ..Collectively, our results are consistent with some degree of quality control by TatBC and a recruitment of TatA to a folded substrate that has functionally engaged the twin-arginine translocase. ..
  4. Gebert M, Schrempp S, Mehnert C, Heißwolf A, Oeljeklaus S, Ieva R, et al. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol. 2012;197:595-604 pubmed publisher
    ..We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import. ..
  5. Kutik S, Stojanovski D, Becker L, Becker T, Meinecke M, Krüger V, et al. Dissecting membrane insertion of mitochondrial beta-barrel proteins. Cell. 2008;132:1011-24 pubmed publisher
    ..We propose that a two-stage mechanism of signal-driven insertion into a membrane protein complex and subsequent integration into the lipid phase may represent a general mechanism for biogenesis of beta-barrel proteins. ..
  6. Dal Bosco C, Dovzhenko A, Palme K. Intracellular auxin transport in pollen: PIN8, PIN5 and PILS5. Plant Signal Behav. 2012;7:1504-5 pubmed publisher
    ..Although our investigation shed light on intracellular auxin transport in pollen, the physiological function of PIN8 still remains to be elucidated. Here we discuss our data taking in consideration other recent findings. ..
  7. Bohnert J, Schuster S, Fähnrich E, Trittler R, Kern W. Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF). J Antimicrob Chemother. 2007;59:1216-22 pubmed
    ..This single point mutation in the periplasmic loop of the pump can increase resistance to a given drug such as a fluoroquinolone while decreasing resistance to another one. ..
  8. Meisinger C, Wiedemann N, Rissler M, Strub A, Milenkovic D, Schönfisch B, et al. Mitochondrial protein sorting: differentiation of beta-barrel assembly by Tom7-mediated segregation of Mdm10. J Biol Chem. 2006;281:22819-26 pubmed
    ..Thus the role of Tom7 is not limited to the TOM complex. Tom7 functions in mitochondrial protein biogenesis by a new mechanism, segregation of a sorting component, leading to a differentiation of beta-barrel assembly. ..
  9. Alami M, Lüke I, Deitermann S, Eisner G, Koch H, Brunner J, et al. Differential interactions between a twin-arginine signal peptide and its translocase in Escherichia coli. Mol Cell. 2003;12:937-46 pubmed
  10. Muller M, Klösgen R. The Tat pathway in bacteria and chloroplasts (review). Mol Membr Biol. 2005;22:113-21 pubmed
    ..Current evidence suggests that a functional Tat translocase is assembled from separate TatBC and TatA assemblies only on demand, i.e., in the presence of transport substrate and a transmembrane H+-motive force. ..
  11. Muller M. Twin-arginine-specific protein export in Escherichia coli. Res Microbiol. 2005;156:131-6 pubmed
    ..Whereas TatC proteins function in the specific recognition of substrate, TatA might be the major pore-forming subunit. ..
  12. Meisinger C, Pfannschmidt S, Rissler M, Milenkovic D, Becker T, Stojanovski D, et al. The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J. 2007;26:2229-39 pubmed
    ..We conclude that assembly of mitochondrial beta-barrel proteins represents a major function of the morphology proteins Mdm12/Mmm1. ..
  13. Holzschuh J, Ryu S, Aberger F, Driever W. Dopamine transporter expression distinguishes dopaminergic neurons from other catecholaminergic neurons in the developing zebrafish embryo. Mech Dev. 2001;101:237-43 pubmed
    ..Neurons expressing th but not dat are adrenergic or noradrenergic, and are found in the locus coeruleus, the medulla, the likely analog of the carotid body, and precursors of the enteric and sympathetic nervous system. ..
  14. Reichelt M, von Specht B, Hahn H. The Caulobacter crescentus outer membrane protein Omp58 (RsaF) is not required for paracrystalline S-layer secretion. FEMS Microbiol Lett. 2001;201:277-83 pubmed
    ..crescentus surface-layer export system. ..
  15. Welte T, Kudva R, Kuhn P, Sturm L, Braig D, Muller M, et al. Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle. Mol Biol Cell. 2012;23:464-79 pubmed publisher
  16. Chacinska A, Pfannschmidt S, Wiedemann N, Kozjak V, Sanjuán Szklarz L, Schulze Specking A, et al. Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J. 2004;23:3735-46 pubmed
    ..We conclude that Mia40 is a central component of the protein import and assembly machinery of the mitochondrial IMS. ..
  17. Rose P, Fröbel J, Graumann P, Muller M. Substrate-dependent assembly of the Tat translocase as observed in live Escherichia coli cells. PLoS ONE. 2013;8:e69488 pubmed publisher
    ..Our collective results are consistent with distinct Tat translocation sites dynamically forming in vivo in response to newly synthesized Tat substrates. ..
  18. Weise A, Lalonde S, K hn C, Frommer W, Ward J. Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells. Plant Mol Biol. 2008;68:251-62 pubmed publisher
    ..The combination of all introns is required for the full expression pattern in phloem, guard cells and trichomes...
  19. Wiedemann N, van der Laan M, Hutu D, Rehling P, Pfanner N. Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain. J Cell Biol. 2007;179:1115-22 pubmed
    ..The motor does not assemble en bloc with the translocase but apparently in a step-wise manner with the Pam16/18 module before the Hsp70 core. ..
  20. Moser M, Panahandeh S, Holzapfel E, Muller M. In vitro analysis of the bacterial twin-arginine-dependent protein export. Methods Mol Biol. 2007;390:63-79 pubmed
    ..This system has proven useful and is being further exploited for the study of precursor-translocase interactions, assembly of the translocase, and the mechanism of transmembrane passage. ..
  21. Wenger C, Oeljeklaus S, Warscheid B, Schneider A, Harsman A. A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase. PLoS Pathog. 2017;13:e1006550 pubmed publisher
    ..Thus, the trypanosomal small Tims play an unexpected and essential role in the biogenesis of the single TIM complex, which for one of them is not linked to import of TbTim17. ..
  22. Gebert N, Chacinska A, Wagner K, Guiard B, Koehler C, Rehling P, et al. Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep. 2008;9:548-54 pubmed publisher
  23. Gebert N, Joshi A, Kutik S, Becker T, McKenzie M, Guan X, et al. Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome. Curr Biol. 2009;19:2133-9 pubmed publisher
    ..Our findings reveal a new role for cardiolipin in protein sorting at the mitochondrial outer membrane and bear implications for the pathogenesis of Barth syndrome. ..
  24. Maurer C, Panahandeh S, Moser M, Muller M. Impairment of twin-arginine-dependent export by seemingly small alterations of substrate conformation. FEBS Lett. 2009;583:2849-53 pubmed publisher
    ..This finding suggests that even incomplete folding of a substrate can interfere with transport by the Tat translocase of Escherichia coli. ..
  25. Bömer U, Rassow J, Zufall N, Pfanner N, Meijer M, Maarse A. The preprotein translocase of the inner mitochondrial membrane: evolutionary conservation of targeting and assembly of Tim17. J Mol Biol. 1996;262:389-95 pubmed
    ..The mechanisms of targeting and assembly were indistinguishable between the Tim17s from distinct organisms, indicating a high evolutionary conservation. ..
  26. Ganas P, Mihasan M, Igloi G, Brandsch R. A two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by Arthrobacter nicotinovorans. Microbiology. 2007;153:1546-55 pubmed publisher
    ..NepAB may function as a metabolic valve by exporting methylamine, the end product of nicotine catabolism, and, in conditions under which it accumulates, the intermediate gamma-N-methylaminobutyrate...
  27. Deitermann S, Sprie G, Koch H. A dual function for SecA in the assembly of single spanning membrane proteins in Escherichia coli. J Biol Chem. 2005;280:39077-85 pubmed
    ..This could suggest that single and multiple spanning membrane proteins are processed differently by the bacterial SecY translocon. ..
  28. Geissler A, Chacinska A, Truscott K, Wiedemann N, Brandner K, Sickmann A, et al. The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell. 2002;111:507-18 pubmed
    ..Thus, Tim50 is crucial for guiding the precursors of matrix proteins to their insertion site in the inner membrane. ..
  29. Koch H, Muller M. Dissecting the translocase and integrase functions of the Escherichia coli SecYEG translocon. J Cell Biol. 2000;150:689-94 pubmed
    ..In addition, the experimental setup used here enabled us to demonstrate that SRP-dependent integration of a multispanning protein into membrane vesicles leads to a biologically active enzyme. ..