Experts and Doctors on spliceosomes in Paris, Île de France, France
Locale: Paris, Île de France, France
Legrain P, Chapon C. Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex. Science. 1993;262:108-10 pubmed
..Structurally and functionally related proteins are found in mammalian cells and are associated in a single biochemical fraction. This strongly suggests that the PRP9-SPP91-PRP11 complex is a key element of the splicing machinery. ..
Legrain P, Chapon C, Galisson F. Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly. Genes Dev. 1993;7:1390-9 pubmed
..Finally, we demonstrate that the PRP9 protein acts after the formation of the U1 snRNP-pre-mRNA complex. The existence of a protein complex including the PRP9 factor is discussed. ..
Galisson F, Legrain P. The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP. Nucleic Acids Res. 1993;21:1555-62 pubmed
..These results establish that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP. We found no evidence for the presence of the PRP6 protein in the U4/U6 particle. ..
Rain J, Tartakoff A, Kramer A, Legrain P. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA. 1996;2:535-50 pubmed
..They do not include the most conserved surp1 module, suggesting that the conservation of this motif in two families of proteins may reflect a still unknown function dispensable in yeast under standard conditions. ..
Minc E, Allory Y, Worman H, Courvalin J, Buendia B. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. Chromosoma. 1999;108:220-34 pubmed
..Thus, subtle differences in primary sequence and in posttranslational modifications may promote their targeting at different chromatin sites, generating pleiotropic effects. ..
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, et al
. Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. Nat Struct Mol Biol. 2012;19:983-90 pubmed publisher
..We elucidated the initial step of EJC assembly and the duality of CWC22 function that hinders eIF4AIII from nonspecifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. ..
Quidville V, Alsafadi S, Goubar A, Commo F, Scott V, Pioche Durieu C, et al
. Targeting the deregulated spliceosome core machinery in cancer cells triggers mTOR blockade and autophagy. Cancer Res. 2013;73:2247-58 pubmed publisher
..These findings provide a rational to develop new therapeutic agents targeting spliceosome core components in oncology. ..
Furney S, Pedersen M, Gentien D, Dumont A, Rapinat A, Desjardins L, et al
. SF3B1 mutations are associated with alternative splicing in uveal melanoma. Cancer Discov. 2013;3:1122-1129 pubmed publisher
..SF3B1 encodes a component of the spliceosome, and RNA sequencing revealed that SF3B1 mutations were associated with differential alternative splicing of protein coding genes, including ABCC5 and UQCC, and of the long noncoding RNA CRNDE...
Bonnet A, Grosso A, Elkaoutari A, Coleno E, Presle A, Sridhara S, et al
. Introns Protect Eukaryotic Genomes from Transcription-Associated Genetic Instability. Mol Cell. 2017;67:608-621.e6 pubmed publisher
..Our results thereby provide a possible rationale for the conservation of introns throughout the eukaryotic lineage. ..