Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Gif sur Yvette, Île de France, France
Locale: Gif sur Yvette, Île de France, France
Topic: saccharomyces cerevisiae
- Nasr F, Bertauche N, Dufour M, Minet M, Lacroute F. Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo py. Mol Gen Genet. 1994;244:23-32 pubmed
- Jia Y, Becam A, Herbert C. The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase. Mol Microbiol. 1997;24:53-9 pubmed..In vitro import experiments showed that cit3p is transported into the mitochondria. Taken together, these data show that the CIT3 gene encodes a second mitochondrial isoform of citrate synthase. ..
- Petitjean A, Bonneaud N, Lacroute F. The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins. Mol Cell Biol. 1995;15:5071-81 pubmed..Polysome profile analysis suggests that the primary defect caused by the depletion in Ssm1p is at the level of translation initiation. ..
- Azevedo D, Tacnet F, Delaunay A, Rodrigues Pousada C, Toledano M. Two redox centers within Yap1 for H2O2 and thiol-reactive chemicals signaling. Free Radic Biol Med. 2003;35:889-900 pubmed..These data indicate that yeast cells cannot sense these compounds through the same molecular devices, albeit they are all electrophilic. ..
- Prouteau M, Daugeron M, Seraphin B. Regulation of ARE transcript 3' end processing by the yeast Cth2 mRNA decay factor. EMBO J. 2008;27:2966-76 pubmed publisher..Consistently, Cth2 localization in the nucleus suggests that it may interfere with poly(A) site selection. Our analysis reveal that ARE-binding protein may affect mRNA 3' end processing and that this contributes to mRNA destabilization. ..
- Rouillard J, Brendolise C, Lacroute F. Rna14p, a component of the yeast nuclear cleavage/polyadenylation factor I, is also localised in mitochondria. Mol Gen Genet. 2000;262:1103-12 pubmed..The localisation data strongly suggest that, besides an essential function in mRNA polyadenylation, the Rna14p protein has a non essential function in mitochondrial metabolism. ..
- Bousset L, Belrhali H, Janin J, Melki R, Morera S. Structure of the globular region of the prion protein Ure2 from the yeast Saccharomyces cerevisiae. Structure. 2001;9:39-46 pubmed..The cap region is highly flexible and may interact with the N-terminal region of the partner subunit in the dimer. The implication of this interaction in the assembly of Ure2p into amyloid fibrils is discussed. ..
- Marsolier Kergoat M. A simple model for the influence of meiotic conversion tracts on GC content. PLoS ONE. 2011;6:e16109 pubmed publisher..Moreover, the model points out to specific constraints on protein fragments encoded by exon terminal sequences, which are the most affected by the GC bias. ..
- Vallieres C, Trouillard M, Dujardin G, Meunier B. Deleterious effect of the Qo inhibitor compound resistance-conferring mutation G143A in the intron-containing cytochrome b gene and mechanisms for bypassing it. Appl Environ Microbiol. 2011;77:2088-93 pubmed publisher..cerevisiae G143A mutant, had a similar compensatory effect. These bypass mechanisms identified in yeast could potentially arise in pathogenic fungi. ..
- Padovani D, Zeghouf M, Traverso J, Giglione C, Cherfils J. High yield production of myristoylated Arf6 small GTPase by recombinant N-myristoyl transferase. Small Gtpases. 2013;4:3-8 pubmed publisher..This establishes in vitro myristoylation as a novel and simple method that could be used to produce other myristoylated Arf and Arf-like GTPases for biochemical assays. ..
- Huet J, Sentenac A. The TATA-binding protein participates in TFIIIB assembly on tRNA genes. Nucleic Acids Res. 1992;20:6451-4 pubmed..The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP. ..
- Thomas D, Barbey R, Henry D, Surdin Kerjan Y. Physiological analysis of mutants of Saccharomyces cerevisiae impaired in sulphate assimilation. J Gen Microbiol. 1992;138:2021-8 pubmed..cerevisiae. Thiosulphate is cleaved into sulphite and sulphide prior to utilization by the sulphate assimilation pathway, as the metabolism of one sulphur atom from thiosulphate requires the presence of an active sulphite reductase. ..
- Nagy M, Lacroute F, Thomas D. Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc Natl Acad Sci U S A. 1992;89:8966-70 pubmed..cerevisiae uses fumarate as terminal electron acceptor. These results are discussed in relation to the anaerobic growth competence of the two yeasts and to the fermentative processes they use. ..
- Panozzo C, Nawara M, Suski C, Kucharczyka R, Skoneczny M, Bécam A, et al. Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae. FEBS Lett. 2002;517:97-102 pubmed..cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph. ..
- Ostojic J, Panozzo C, Lasserre J, Nouet C, Courtin F, Blancard C, et al. The energetic state of mitochondria modulates complex III biogenesis through the ATP-dependent activity of Bcs1. Cell Metab. 2013;18:567-77 pubmed publisher..Our study further highlights the intramitochondrial adenine nucleotide pool as a potential target for the treatment of Bcs1-based disorders. ..
- Rieger K, Lazowska J, Pohl T, Slonimski P, Aljinovic G. A novel nuclear gene, CBT1, essential for mitochondrial cytochrome b formation: terminal processing of mRNA and intron dependence. Curr Genet. 1997;32:163-74 pubmed..We propose, that the CBT1 protein is necessary for the correct trimming of the end of cytb pre-mRNA and may be a part of the multi-component complex involved in this process. ..
- Racki W, Becam A, Nasr F, Herbert C. Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. EMBO J. 2000;19:4524-32 pubmed
- Li de la Sierra Gallay I, Collinet B, Graille M, Quevillon Cheruel S, Liger D, Minard P, et al. Crystal structure of the YGR205w protein from Saccharomyces cerevisiae: close structural resemblance to E. coli pantothenate kinase. Proteins. 2004;54:776-83 pubmed..The nature of the phosphate accepting substrate remains to be determined. ..
- Delaunay A, Pflieger D, Barrault M, Vinh J, Toledano M. A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation. Cell. 2002;111:471-81 pubmed..These data reveal a redox-signaling function for a GPx-like enzyme and elucidate a eukaryotic hydroperoxide-sensing mechanism. Gpx3 is thus a hydroperoxide receptor and redox-transducer. ..
- Thomas D, Surdin Kerjan Y. Structure of the HOM2 gene of Saccharomyces cerevisiae and regulation of its expression. Mol Gen Genet. 1989;217:149-54 pubmed..We have found that ASA DH is the first reported enzyme of the related threonine and methionine pathway to be regulated by the general control of amino acid synthesis. ..
- Merlot S, Leonhardt N, Fenzi F, Valon C, Costa M, Piette L, et al. Constitutive activation of a plasma membrane H(+)-ATPase prevents abscisic acid-mediated stomatal closure. EMBO J. 2007;26:3216-26 pubmed
- Ghislain M, Udvardy A, Mann C. S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase. Nature. 1993;366:358-62 pubmed..The CLB2 and CLB3 cyclins also accumulate in the cim mutants. Thus the 26S protease is required in vivo for the degradation of ubiquitinated substrates and for anaphase chromosome separation. ..
- Ozier Kalogeropoulos O, Adeline M, Yang W, Carman G, Lacroute F. Use of synthetic lethal mutants to clone and characterize a novel CTP synthetase gene in Saccharomyces cerevisiae. Mol Gen Genet. 1994;242:431-9 pubmed..Nevertheless, URA8 alone also allows yeast growth, at least under standard laboratory conditions. ..
- Groudinsky O, Bousquet I, Wallis M, Slonimski P, Dujardin G. The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae. Mol Gen Genet. 1993;240:419-27 pubmed..The abundance of transcripts from five other genes was either slightly (21S rRNA) or not at all (cox2, cox3, atp9 and 15S rRNA) affected by the nam1 inactivation.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Duport C, Schoepp B, Chatelain E, Spagnoli R, Dumas B, Pompon D. Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast. Eur J Biochem. 2003;270:1502-14 pubmed..Thus, the observation that the highest cellular levels of free ergosta-5-eneol are found in the plasma membrane suggests that the substrate is freely available for pregnenolone synthesis. ..
- Rouillon A, Surdin Kerjan Y, Thomas D. Transport of sulfonium compounds. Characterization of the s-adenosylmethionine and s-methylmethionine permeases from the yeast Saccharomyces cerevisiae. J Biol Chem. 1999;274:28096-105 pubmed
- Thomas D, Surdin Kerjan Y. SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression. J Biol Chem. 1987;262:16704-9 pubmed..The DNA sequence of SAM1 is reported. The translation product shows a high homology with the one deduced from the sequence of the MetK gene encoding the SAM synthetase of Escherichia coli. ..
- Minet M, Dufour M, Lacroute F. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J. 1992;2:417-22 pubmed..The cDNA complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenase sequence. ..
- Walbott H, Auxilien S, Grosjean H, Golinelli Pimpaneau B. The carboxyl-terminal extension of yeast tRNA m5C methyltransferase enhances the catalytic efficiency of the amino-terminal domain. J Biol Chem. 2007;282:23663-71 pubmed..It is not required for m(5)C formation and does not appear to contribute to substrate specificity. However, it enhances considerably the catalytic efficiency of the amino-terminal domain. ..
- Rougemaille M, Gudipati R, Olesen J, Thomsen R, Seraphin B, Libri D, et al. Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. EMBO J. 2007;26:2317-26 pubmed
- Cherest H, Davidian J, Thomas D, Benes V, Ansorge W, Surdin Kerjan Y. Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae. Genetics. 1997;145:627-35 pubmed..Sul3p has been shown to be involved in the transcriptional regulation of the SUL2 gene. ..
- Peyroche A, Paris S, Jackson C. Nucleotide exchange on ARF mediated by yeast Gea1 protein. Nature. 1996;384:479-81 pubmed..We propose that Gea1 and ARNO, a human protein with a homologous Sec7 domain, are members of a new family of ARF guanine-nucleotide exchange factors. ..
- Mazzoni C, Zarov P, Rambourg A, Mann C. The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae. J Cell Biol. 1993;123:1821-33 pubmed..Furthermore, slt2::HIS3 act1-1 and slt2::HIS3 myo2-66 double mutants are inviable. We suggest that Slt2p functions downstream or in parallel with Cdc28p in promoting bud formation and apical growth. ..
- Hlavacek O, Bourens M, Salone V, Lachacinski N, Lemaire C, Dujardin G. The transcriptional activator HAP4 is a high copy suppressor of an oxa1 yeast mutation. Gene. 2005;354:53-7 pubmed..We show that the suppressor gene corresponds to the nuclear transcriptional activator Hap4p which is known to regulate respiratory functions. ..
- Szczepanek T, Lazowska J. Replacement of two non-adjacent amino acids in the S.cerevisiae bi2 intron-encoded RNA maturase is sufficient to gain a homing-endonuclease activity. EMBO J. 1996;15:3758-67 pubmed..capensis bi2-encoded protein which functions in both splicing and intron mobility in the wild-type cells. These results provide new insight into our understanding of the activity and the evolution of group I intron-encoded proteins. ..
- Thomas D, Kuras L, Barbey R, Cherest H, Blaiseau P, Surdin Kerjan Y. Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol. 1995;15:6526-34 pubmed..By the two-hybrid method, we showed that Met30p interacts with Met4p and identified a region of Met4p involved in this interaction. Further analysis reveals that expression of Met30p is essential for cell viability. ..
- Lancelot N, Charier G, Couprie J, Duband Goulet I, Alpha Bazin B, Quemeneur E, et al. The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA. Nucleic Acids Res. 2007;35:5898-912 pubmed..However, their modes of chromatin recognition are different, suggesting that the corresponding interactions are differently regulated. ..
- Dubacq C, Chevalier A, Mann C. The protein kinase Snf1 is required for tolerance to the ribonucleotide reductase inhibitor hydroxyurea. Mol Cell Biol. 2004;24:2560-72 pubmed..Quantitative regulation of Snf1 kinase activity may contribute to the specificity of the effector responses that it controls. ..
- Nouet C, Bourens M, Hlavacek O, Marsy S, Lemaire C, Dujardin G. Rmd9p controls the processing/stability of mitochondrial mRNAs and its overexpression compensates for a partial deficiency of oxa1p in Saccharomyces cerevisiae. Genetics. 2007;175:1105-15 pubmed
- Dubacq C, Chevalier A, Courbeyrette R, Petat C, Gidrol X, Mann C. Role of the iron mobilization and oxidative stress regulons in the genomic response of yeast to hydroxyurea. Mol Genet Genomics. 2006;275:114-24 pubmed..These results highlight the importance of the redox and iron mobilization regulons in the cellular response to HU. ..
- van Dijk E, Le Hir H, Seraphin B. DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway. Proc Natl Acad Sci U S A. 2003;100:12081-6 pubmed..m7GDP breakdown should prevent misincorporation of methylated nucleotides in nucleic acids and could generate a unique indicator allowing the cell to monitor mRNA decay. ..
- Bourens M, Panozzo C, Nowacka A, Imbeaud S, Mucchielli M, Herbert C. Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism. Genetics. 2009;183:161-73 pubmed publisher..Our experiments reveal a multilayered system controlling aspects of cell separation, cell integrity, mating, and polarized growth. ..
- David Bosne S, Florent I, Lund Winther A, Hansen J, Buch Pedersen M, Machillot P, et al. Antimalarial screening via large-scale purification of Plasmodium falciparum Ca2+-ATPase 6 and in vitro studies. FEBS J. 2013;280:5419-29 pubmed publisher..The present study describes a multidisciplinary approach allowing the selection of promising PfATP6-specific inhibitors with good antimalarial activity. ..
- Arrebola R, Manaud N, Rozenfeld S, Marsolier M, Lefebvre O, Carles C, et al. Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding. Mol Cell Biol. 1998;18:1-9 pubmed..These results indicated that tau91 cooperates with tau138 for DNA binding. Recombinant tau91 by itself did not interact with a tRNA gene, although it showed a strong affinity for single-stranded DNA. ..
- Jackson C, Casanova J. Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors. Trends Cell Biol. 2000;10:60-7 pubmed..A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals. ..
- Hamel P, Sakamoto W, Wintz H, Dujardin G. Functional complementation of an oxa1- yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J. 1997;12:1319-27 pubmed..Our results suggest that the Oxa1At protein is essential for the respiratory complex assembly in A. thaliana, and that genes involved in mitochondrial multiprotein complex formation can be conserved between plants and other organisms. ..
- Ozier Kalogeropoulos O, Fasiolo F, Adeline M, Collin J, Lacroute F. Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase. Mol Gen Genet. 1991;231:7-16 pubmed..This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate. ..
- Minet M, Lacroute F. Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae. Curr Genet. 1990;18:287-91 pubmed..subtilis AIR carboxylase (E.C.188.8.131.52). In agreement with these homologies, pADE2H1 clones complement both ade1 and ade2 mutants of S. cerevisiae, as was also recently reported for a 3.1 kb cDNA isolated from human hepatocytes. ..
- Amrani N, Dufour M, Bonneaud N, Lacroute F. Mutations in STS1 suppress the defect in 3' mRNA processing caused by the rna15-2 mutation in Saccharomyces cerevisiae. Mol Gen Genet. 1996;252:552-62 pubmed..Use of the two-hybrid system suggests that Sts1p does not interact directly with Rna15p, but may be active as a homodimer. The present data suggest that Sts1p may play a role in the transport of Rna15p from the cytoplasm to the nucleus...
- Linder P, Slonimski P. An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. Proc Natl Acad Sci U S A. 1989;86:2286-90 pubmed..Inactivation of either gene by gene disruption has no effect on cell viability or on mitochondrial functions. However, simultaneous inactivation of both genes is lethal to the cell. ..
- Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed..The possible mechanisms by which the NAM1 gene product may function are discussed. ..
- Shpakovski G, Acker J, Wintzerith M, Lacroix J, Thuriaux P, Vigneron M. Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:4702-10 pubmed..Finally, a doubly chimeric S. cerevisiae strain bearing the Sp6 cDNA and the human Hs10 beta cDNA was also viable. No interspecific complementation was observed for the human hRPB25 (Hs5) homolog of the yeast ABC27 (Sc5) subunit. ..
- Auzat I, Le Bras G, Branny P, de la Torre F, Theunissen B, Garel J. The role of Glu187 in the regulation of phosphofructokinase by phosphoenolpyruvate. J Mol Biol. 1994;235:68-72 pubmed..This requirement of a negative charge for ADP binding could explain the striking conservation of an aspartate residue at position 187 in all the eukaryotic phosphofructokinases. ..
- Leroy C, Lee S, Vaze M, Ochsenbein F, Ochsenbien F, Guerois R, et al. PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell. 2003;11:827-35 pubmed..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53. ..
- Mann C, Micouin J, Chiannilkulchai N, Treich I, Buhler J, Sentenac A. RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest. Mol Cell Biol. 1992;12:4314-26 pubmed..The BN51 cDNA was originally isolated by its ability to complement a temperature-sensitive hamster cell mutant that undergoes a G1 cell division arrest, as is true for the rpc53 mutants. ..
- Marsolier Kergoat M. Asymmetry indices for analysis and prediction of replication origins in eukaryotic genomes. PLoS ONE. 2012;7:e45050 pubmed publisher..However, I show here that the skew jumps at C. albicans centromeres are not related to replication and that replication-associated GC and TA skews in C. albicans have in fact the opposite directions of what was proposed. ..
- Albertin W, Marullo P. Polyploidy in fungi: evolution after whole-genome duplication. Proc Biol Sci. 2012;279:2497-509 pubmed publisher..In particular, the genus Saccharomyces emerges as a relevant model for polyploid studies, in addition to plant and animal models. ..
- Saulou C, Jamme F, Maranges C, Fourquaux I, Despax B, Raynaud P, et al. Synchrotron FTIR microspectroscopy of the yeast Saccharomyces cerevisiae after exposure to plasma-deposited nanosilver-containing coating. Anal Bioanal Chem. 2010;396:1441-50 pubmed publisher..For control experiments with the organosilicon matrix alone, no antimicrobial effect was observed, which was consistent with synchrotron FTIR results and TEM observations. ..
- Cottrelle P, Cool M, Thuriaux P, Price V, Thiele D, Buhler J, et al. Either one of the two yeast EF-1 alpha genes is required for cell viability. Curr Genet. 1985;9:693-7 pubmed..In contrast, attempts to isolate a yeast haploid strain with both TEF1 and TEF2 inactivated have failed suggesting that the double gene disruption is a lethal event. ..
- Le Tallec B, Barrault M, Courbeyrette R, Guerois R, Marsolier Kergoat M, Peyroche A. 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell. 2007;27:660-74 pubmed..Our findings provide evidence for a remarkable conservation of a pairwise chaperone-assisted proteasome assembly throughout evolution. ..
- Rousselet G, Simon M, Ripoche P, Buhler J. A second nitrogen permease regulator in Saccharomyces cerevisiae. FEBS Lett. 1995;359:215-9 pubmed..Our data suggest that this protein is a post-transcriptional regulator of nitrogen permeases. ..
- Harington A, Schwarz E, Slonimski P, Herbert C. Subcellular relocalization of a long-chain fatty acid CoA ligase by a suppressor mutation alleviates a respiration deficiency in Saccharomyces cerevisiae. EMBO J. 1994;13:5531-8 pubmed..These results support the hypothesis that some form of fatty acid synthesis, specific for the mitochondria, is essential for the function of the organelle. ..
- Harington A, Herbert C, Tung B, Getz G, Slonimski P. Identification of a new nuclear gene (CEM1) encoding a protein homologous to a beta-keto-acyl synthase which is essential for mitochondrial respiration in Saccharomyces cerevisiae. Mol Microbiol. 1993;9:545-55 pubmed..Thus it is possible that the CEM1 protein is involved in the synthesis of a specialized molecule, probably related to a fatty acid, which is essential for mitochondrial respiration. ..
- Thuret J, Valay J, Faye G, Mann C. Civ1 (CAK in vivo), a novel Cdk-activating kinase. Cell. 1996;86:565-76 pubmed..Civ1 is the only CAK for which there are genetic data indicating that its activity is physiologically relevant in vivo. ..
- Gadal O, Mariotte Labarre S, Chedin S, Quemeneur E, Carles C, Sentenac A, et al. A34.5, a nonessential component of yeast RNA polymerase I, cooperates with subunit A14 and DNA topoisomerase I to produce a functional rRNA synthesis machine. Mol Cell Biol. 1997;17:1787-95 pubmed..A34.5 (but not A14) becomes quasi-essential in strains lacking DNA topoisomerase I, suggesting a specific role of this subunit in helping Pol I to overcome the topological constraints imposed on ribosomal DNA by transcription. ..
- Amrani N, Minet M, Le Gouar M, Lacroute F, Wyers F. Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. Mol Cell Biol. 1997;17:3694-701 pubmed..Our data indicate that Pab1 is also a part of the 3'-end RNA-processing complex and thus participates in the control of the poly(A) tail lengths during the polyadenylation reaction. ..
- Vo L, Minet M, Schmitter J, Lacroute F, Wyers F. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol. 2001;21:8346-56 pubmed..These results show that Mpe1p plays a crucial role in 3' end formation probably by promoting the specific link between the CFI/CPF complex and pre-mRNA. ..
- Daniel J. Direct in vivo access to potential gene targets of the RPD3 histone deactylase using fitness-based interferential genetics. Yeast. 2007;24:575-87 pubmed
- Pompon D. cDNA cloning and functional expression in yeast Saccharomyces cerevisiae of beta-naphthoflavone-induced rabbit liver P-450 LM4 and LM6. Eur J Biochem. 1988;177:285-93 pubmed..Enzymatic and evolutionary implications of these results are discussed. ..
- Pinskaya M, Gourvennec S, Morillon A. H3 lysine 4 di- and tri-methylation deposited by cryptic transcription attenuates promoter activation. EMBO J. 2009;28:1697-707 pubmed publisher..Our data support a model wherein certain promoters are embedded in a repressive chromatin controlled by cryptic transcription. ..
- Lecain E, Chenivesse X, Spagnoli R, Pompon D. Cloning by metabolic interference in yeast and enzymatic characterization of Arabidopsis thaliana sterol delta 7-reductase. J Biol Chem. 1996;271:10866-73 pubmed..In vitro tests indicated that delta 7-reductase activity is preferentially associated with the endoplasmic reticulum membrane and confirmed the previous finding that NADPH is the reducing agent. ..
- Li G, Becam A, Slonimski P, Herbert C. In vitro mutagenesis of the mitochondrial leucyl tRNA synthetase of Saccharomyces cerevisiae shows that the suppressor activity of the mutant proteins is related to the splicing function of the wild-type protein. Mol Gen Genet. 1996;252:667-75 pubmed..These data show that the splicing and suppressor functions are linked and that the suppressor activity of the mutant alleles results from a modification of the wild-type splicing activity. ..
- Marsolier Kergoat M, Goldar A. DNA replication induces compositional biases in yeast. Mol Biol Evol. 2012;29:893-904 pubmed publisher..Taken together, our results suggest that replication-related compositional biases may be a feature of many eukaryotic genomes despite the stochastic nature of the firing of replication origins in these genomes. ..
- Cardazzo B, Hamel P, Sakamoto W, Wintz H, Dujardin G. Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity. Gene. 1998;221:117-25 pubmed..thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function. ..
- Bousquet I, Dujardin G, Slonimski P. ABC1, a novel yeast nuclear gene has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex. EMBO J. 1991;10:2023-31 pubmed
- Stettler S, Mariotte S, Riva M, Sentenac A, Thuriaux P. An essential and specific subunit of RNA polymerase III (C) is encoded by gene RPC34 in Saccharomyces cerevisiae. J Biol Chem. 1992;267:21390-5 pubmed..The latter phenotype was observed for all conditionally defective RNA polymerase III mutants isolated so far. ..