Genomes and Genes
Experts and Doctors on molecular sequence data in Gif sur Yvette, Île de France, France
Locale: Gif sur Yvette, Île de France, France
Topic: molecular sequence data
Publications135 found, 100 shown here
- Morel N, Dedieu J, Philippe J. Specific sorting of the a1 isoform of the V-H+ATPase a subunit to nerve terminals where it associates with both synaptic vesicles and the presynaptic plasma membrane. J Cell Sci. 2003;116:4751-62 pubmed..This V-ATPase interacts with VAMP-2 and with the SNARE complexes involved in synaptic vesicle docking and exocytosis. ..
- Weill L, Louis D, Sargueil B. Selection and evolution of NTP-specific aptamers. Nucleic Acids Res. 2004;32:5045-58 pubmed..The characteristics of these aptamers are discussed along with implications for in vitro molecular evolution. ..
- Chiron S, Suleau A, Bonnefoy N. Mitochondrial translation: elongation factor tu is essential in fission yeast and depends on an exchange factor conserved in humans but not in budding yeast. Genetics. 2005;169:1891-901 pubmed..We find that S. cerevisiae EF-Tu is functionally equivalent to the S. pombe EF-Tu/EF-Ts couple. Point mutations in the S. pombe EF-Tu can render it independent of its exchange factor, thereby mimicking the situation in S. cerevisiae. ..
- D Angelo Picard C, Faure D, Penot I, Dessaux Y. Diversity of N-acyl homoserine lactone-producing and -degrading bacteria in soil and tobacco rhizosphere. Environ Microbiol. 2005;7:1796-808 pubmed
- Jézéquel L, Loeper J, Pompon D. Sequence-independent construction of ordered combinatorial libraries with predefined crossover points. Biotechniques. 2008;45:523-32 pubmed publisher..Fragments are finally reassembled using a fusion PCR. The process is illustrated via the construction of a set of mosaic CYP2B enzymes using this highly modular approach. ..
- Cortial S, Chaignon P, Iorga B, Aymerich S, Truan G, Gueguen Chaignon V, et al. NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role. FEBS Lett. 2010;584:3916-22 pubmed publisher..Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously. ..
- Mirabeau O, Joly J. Molecular evolution of peptidergic signaling systems in bilaterians. Proc Natl Acad Sci U S A. 2013;110:E2028-37 pubmed publisher..In addition to shedding light on the function of human G protein-coupled receptor PSs, this work presents orthology markers to study ancestral neuron types that were probably present in the last common bilaterian ancestor. ..
- Viel A, le Maire M, Philippe H, Morales J, Mazabraud A, Denis H. Structural and functional properties of thesaurin a (42Sp50), the major protein of the 42 S particles present in Xenopus laevis previtellogenic oocytes. J Biol Chem. 1991;266:10392-9 pubmed..In particular, the 42 S particle contains GTP and efficiently transfers aminoacyl-tRNA to 80 S ribosomes without addition of exogenous elongation factor. ..
- Teschner W, Serre M, Garel J. Enzymatic properties, renaturation and metabolic role of mannitol-1-phosphate dehydrogenase from Escherichia coli. Biochimie. 1990;72:33-40 pubmed..The enzymatic properties of isolated D-mannitol-1-phosphate dehydrogenase are discussed in relation to the role of this enzyme in the intracellular metabolism. ..
- Lalo D, Carles C, Sentenac A, Thuriaux P. Interactions between three common subunits of yeast RNA polymerases I and III. Proc Natl Acad Sci U S A. 1993;90:5524-8 pubmed..These results suggest that the assembly of RNA polymerase I and III requires the association of ABC10 beta subunit with an AC19/AC40 heterodimer. ..
- Kamoun C, Payen T, Hua Van A, Filee J. Improving prokaryotic transposable elements identification using a combination of de novo and profile HMM methods. BMC Genomics. 2013;14:700 pubmed publisher..For metagenomic data, profile HMM search should be favored, a BLAST-based step is only useful to the final annotation into groups and families. ..
- Gillet D, Ducancel F, Pradel E, Leonetti M, Menez A, Boulain J. Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities. Protein Eng. 1992;5:273-8 pubmed..We propose to use the system described in this paper for fast preparation of properly folded disulfide-containing enzymatic probes. ..
- Mary J, Redeker V, Le Caer J, Prome J, Rossier J. Class I and IVa beta-tubulin isotypes expressed in adult mouse brain are glutamylated. FEBS Lett. 1994;353:89-94 pubmed..Anionic peptides were then characterized by amino acid sequencing and mass spectrometry. We show that brain-specific class IVa and constitutive class I beta-tubulin isotypes can be glutamylated, at Glu434 and Glu441, respectively. ..
- Thomas D, Kuras L, Barbey R, Cherest H, Blaiseau P, Surdin Kerjan Y. Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol. 1995;15:6526-34 pubmed..By the two-hybrid method, we showed that Met30p interacts with Met4p and identified a region of Met4p involved in this interaction. Further analysis reveals that expression of Met30p is essential for cell viability. ..
- Garreau de Loubresse N, Ruiz F, Beisson J, Klotz C. Role of delta-tubulin and the C-tubule in assembly of Paramecium basal bodies. BMC Cell Biol. 2001;2:4 pubmed..Delta-tubulin homologs have been identified in the genomes of mammals and protozoa, but their phylogenetic relationships are unclear and their function is not yet known...
- Graille M, Stura E, Housden N, Beckingham J, Bottomley S, Beale D, et al. Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins. Structure. 2001;9:679-87 pubmed..It consists of up to five homologous immunoglobulin binding domains that interact with the variable (VL) regions of kappa light chains found on two thirds of mammalian antibodies...
- Lescop E, Briand L, Pernollet J, Guittet E. Structural basis of the broad specificity of a general odorant-binding protein from honeybee. Biochemistry. 2009;48:2431-41 pubmed publisher..We propose that the broad ligand specificity of GOBPs compared with PBPs is conferred by the cumulative effects of weak nonspecific protein-ligand interactions and of enhanced protein internal dynamics at the ligand entry site. ..
- Bontems F, Gilquin B, Roumestand C, Menez A, Toma F. Analysis of side-chain organization on a refined model of charybdotoxin: structural and functional implications. Biochemistry. 1992;31:7756-64 pubmed..Thus, charybdotoxin and scyllatoxin, which present the same global fold, interact with two different classes of K+ channels by two different parts of the motif. ..
- Minvielle Sebastia L, Winsor B, Bonneaud N, Lacroute F. Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein. Mol Cell Biol. 1991;11:3075-87 pubmed
- Powell J, Hsu Y, Weyler W, Chen S, Salach J, Andrikopoulos K, et al. The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes. Biochem J. 1989;259:407-13 pubmed..Analysis of mRNAs coding for MAO enzymes showed a heterogeneity of transcripts consistent with several different forms of monoamine oxidase. ..
- Le Bourdelles B, Boularand S, Boni C, Horellou P, Dumas S, Grima B, et al. Analysis of the 5' region of the human tyrosine hydroxylase gene: combinatorial patterns of exon splicing generate multiple regulated tyrosine hydroxylase isoforms. J Neurochem. 1988;50:988-91 pubmed..Comparison of the 5' flanking regions of human and rat genes discloses several highly conserved segments, likely to play an important role in the regulation of TH gene expression. ..
- Lamouroux A, Vigny A, Faucon Biguet N, Darmon M, Franck R, Henry J, et al. The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme. EMBO J. 1987;6:3931-7 pubmed..Comparative amino acid sequence analysis establishes that DBH shares no homology with the other catecholamine synthesizing enzymes, tyrosine hydroxylase and phenylethanolamine-N-methyl transferase. ..
- Marck C, Lefebvre O, Carles C, Riva M, Chaussivert N, Ruet A, et al. The TFIIIB-assembling subunit of yeast transcription factor TFIIIC has both tetratricopeptide repeats and basic helix-loop-helix motifs. Proc Natl Acad Sci U S A. 1993;90:4027-31 pubmed..A motif akin to the basic helix-loop-helix motif of MyoD suggests the direct involvement of tau 131 in promoting DNA binding of TFIIIB. ..
- Potier M, Chelot E, Pekarsky Y, Gardiner K, Rossier J, Turnell W. The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21. Genomics. 1995;29:562-70 pubmed..The chromosomal localization of the gene for human MLCK is shown to be at 3qcen-q21, as determined by PCR and Southern blotting using two somatic cell hybrid panels. ..
- Amrani N, Minet M, Wyers F, Dufour M, Aggerbeck L, Lacroute F. PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I. Mol Cell Biol. 1997;17:1102-9 pubmed..Moreover, fractions obtained by anion-exchange chromatography of extracts from the wild-type strain contain both Pcf11 and Rna15 in the same fractions, as shown by immunoblotting with a Pcf11-specific antibody. ..
- Blaiseau P, Isnard A, Surdin Kerjan Y, Thomas D. Met31p and Met32p, two related zinc finger proteins, are involved in transcriptional regulation of yeast sulfur amino acid metabolism. Mol Cell Biol. 1997;17:3640-8 pubmed..These results therefore emphasise the diversity of the mechanisms allowing regulation of the expression of the methionine biosynthetic genes. ..
- Boquet I, Boujemaa R, Carlier M, Preat T. Ciboulot regulates actin assembly during Drosophila brain metamorphosis. Cell. 2000;102:797-808 pubmed..Genetic experiments show that Cib and the Drosophila profilin protein Chickadee (Chic) cooperate in central brain metamorphosis. ..
- Bichon A, Boukhatem N, Gay P, Dru P, Terzian H, Petitjean A, et al. Genetic and molecular features of Su(P), a gene that interacts with ref(2)P in male fertility of Drosophila melanogaster. Mol Genet Genomics. 2001;265:354-61 pubmed..The Su(P) gene was cloned by P element tagging. The P element-tagged alleles identified a Su(P) transcript as a 1.4-kb mRNA produced in the soma of both males and females, which is also abundant in ovaries. ..
- Messageot F, Salhi S, Eon P, Rossignol J. Proteolytic processing of the hepatitis B virus e antigen precursor. Cleavage at two furin consensus sequences. J Biol Chem. 2003;278:891-5 pubmed..In addition, we show that in our experimental system, the HBeAg is a 164-amino acid protein and not a 159-amino acid protein as previously reported. ..
- González Sama A, de la Peña T, Kevei Z, Mergaert P, Lucas M, de Felipe M, et al. Nuclear DNA endoreduplication and expression of the mitotic inhibitor Ccs52 associated to determinate and lupinoid nodule organogenesis. Mol Plant Microbe Interact. 2006;19:173-80 pubmed..In view of our results, Ccs52A-mediated endoreduplication appears to be a universal mechanism required for nodule cell differentiation during the establishment of nitrogen-fixing symbioses. ..
- Duquerroy S, Stura E, Bressanelli S, Fabiane S, Vaney M, Beale D, et al. Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturation. J Mol Biol. 2007;368:1321-31 pubmed..The antibody contacts also involve two somatically mutated V(H) residues, reinforcing the suggestion of a process of antigen-driven maturation and selection for IgG Fc during the generation of this RF autoantibody. ..
- Van de Vrede Y, Fossier P, Baux G, Joels M, Chameau P. Control of IsAHP in mouse hippocampus CA1 pyramidal neurons by RyR3-mediated calcium-induced calcium release. Pflugers Arch. 2007;455:297-308 pubmed..Our results argue in favor of a specialized function of RyR3 in CA1 pyramidal cells in triggering IsAHP due to their localization in the apical dendrite. ..
- Hua Van A, Capy P. Analysis of the DDE motif in the Mutator superfamily. J Mol Evol. 2008;67:670-81 pubmed publisher..The characteristics of this motif are depicted in each known family of MULEs. Different hypotheses about the evolution of this triad are discussed. ..
- Laporte P, Satiat Jeunemaitre B, Velasco I, Csorba T, Van de Velde W, Campalans A, et al. A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis. Plant J. 2010;62:24-38 pubmed publisher..MtSNARP2 RNA interference (RNAi) transgenic roots showed aberrant early senescent nodules where differentiated bacteroids degenerate rapidly. Hence, a functional symbiotic interaction may be regulated by secreted RNA-binding peptides. ..
- Sirichandra C, Davanture M, Turk B, Zivy M, Valot B, Leung J, et al. The Arabidopsis ABA-activated kinase OST1 phosphorylates the bZIP transcription factor ABF3 and creates a 14-3-3 binding site involved in its turnover. PLoS ONE. 2010;5:e13935 pubmed publisher..In a wider physiological context, we propose that the long term responses to ABA that require sustained gene expression is, in part, mediated by the stabilization of ABFs driven by ABA-activated SnRK2s. ..
- Albertin W, Marullo P, Bely M, Aigle M, Bourgais A, Langella O, et al. Linking post-translational modifications and variation of phenotypic traits. Mol Cell Proteomics. 2013;12:720-35 pubmed publisher..These results highlight the importance of post-translational modifications in the diversity of metabolic and life-history traits. ..
- BarthÃ©lemy N, Fenaille F, Hirtz C, Sergeant N, Schraen Maschke S, Vialaret J, et al. Tau Protein Quantification in Human Cerebrospinal Fluid by Targeted Mass Spectrometry at High Sequence Coverage Provides Insights into Its Primary Structure Heterogeneity. J Proteome Res. 2016;15:667-76 pubmed publisher..This MS-based strategy provided results complementary to those of previous ELISA or Western Blot studies of CSF tau and could be applied to tau monitoring in human CSF cohorts. ..
- Frénois F, Legrand P, Fribourg S. Sqt1p is an eight-bladed WD40 protein. Acta Crystallogr F Struct Biol Commun. 2016;72:59-64 pubmed publisher..On the basis of the atomic structure of Sqt1p, the potential residues involved in uL16 interaction were identified and tested. ..
- Thomas D, Surdin Kerjan Y. Structure of the HOM2 gene of Saccharomyces cerevisiae and regulation of its expression. Mol Gen Genet. 1989;217:149-54 pubmed..We have found that ASA DH is the first reported enzyme of the related threonine and methionine pathway to be regulated by the general control of amino acid synthesis. ..
- Bucheton A. I transposable elements and I-R hybrid dysgenesis in Drosophila. Trends Genet. 1990;6:16-21 pubmed..The mechanism and control of this process are now being dissected at the molecular level. ..
- Dje M, Mazabraud A, Viel A, le Maire M, Denis H, Crawford E, et al. Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis. Nucleic Acids Res. 1990;18:3489-93 pubmed..The latter two proteins are very similar to known eukaryotic EF-1 alpha from other organisms and presumably function in their respective cell types to support protein synthesis. ..
- Chaminade M, Chelot E, Prado De Carvalho L, Bochet P, Rossier J. Cat proenkephalin-A does not contain the opioid octapeptide. Neurochem Int. 1996;28:155-60 pubmed..Furthermore, the peptide is not flanked by paired basic residues, suggesting it is not processed. ..
- Siatecka M, Rozek M, Barciszewski J, Mirande M. Modular evolution of the Glx-tRNA synthetase family--rooting of the evolutionary tree between the bacteria and archaea/eukarya branches. Eur J Biochem. 1998;256:80-7 pubmed..The transient capture of UAA and UAG codons could have favored the emergence of a GlnRS in early eukaryotes. ..
- Traiffort E, Ruat M, O Regan S, Meunier F. Molecular characterization of the family of choline transporter-like proteins and their splice variants. J Neurochem. 2005;92:1116-25 pubmed..These findings suggest specific roles for CTL1 splice variants in both neuronal and oligodendrocyte physiology. ..
- Zaros C, Briand J, Boulard Y, Labarre Mariotte S, Garcia Lopez M, Thuriaux P, et al. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases. Nucleic Acids Res. 2007;35:634-47 pubmed
- Walbott H, Auxilien S, Grosjean H, Golinelli Pimpaneau B. The carboxyl-terminal extension of yeast tRNA m5C methyltransferase enhances the catalytic efficiency of the amino-terminal domain. J Biol Chem. 2007;282:23663-71 pubmed..It is not required for m(5)C formation and does not appear to contribute to substrate specificity. However, it enhances considerably the catalytic efficiency of the amino-terminal domain. ..
- Ben Amor B, Wirth S, Merchan F, Laporte P, d Aubenton Carafa Y, Hirsch J, et al. Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses. Genome Res. 2009;19:57-69 pubmed publisher..Hence, together with small RNAs, long npcRNAs encompass a sensitive component of the transcriptome that have diverse roles during growth and differentiation. ..
- Benassi V, Aulard S, Mazeau S, Veuille M. Molecular variation of Adh and P6 genes in an African population of Drosophila melanogaster and its relation to chromosomal inversions. Genetics. 1993;134:789-99 pubmed..Their relation with In(2L)t supports the hypothesis that WaS recently recombined away from an In(2L)t chromosome which may be the cause of its divergence from the other haplotypes. ..
- Timchenko T, Katul L, Sano Y, de Kouchkovsky F, Vetten H, Gronenborn B. The master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. Virology. 2000;274:189-95 pubmed publisher..Such cross-species replication may reflect a considerable potential for genetic reassortment among nanoviruses in nature and be of significance for their evolution...
- Le Masson M, Baharoglu Z, Michel B. ruvA and ruvB mutants specifically impaired for replication fork reversal. Mol Microbiol. 2008;70:537-48 pubmed..These results show that a partial RuvA or RuvB defect affects primarily RFR, implying that RFR is a more demanding reaction than Holliday junction resolution. ..
- Boulay C, Wilson A, D Haene S, Kirilovsky D. Identification of a protein required for recovery of full antenna capacity in OCP-related photoprotective mechanism in cyanobacteria. Proc Natl Acad Sci U S A. 2010;107:11620-5 pubmed publisher..The discovery of FRP and its characterization are essential elements in the understanding of the OCP-related photoprotective mechanism in cyanobacteria. ..
- Dequard Chablat M, Riva M, Carles C, Sentenac A. RPC19, the gene for a subunit common to yeast RNA polymerases A (I) and C (III). J Biol Chem. 1991;266:15300-7 pubmed..The 5'-upstream region contains a sequence element, the PAC box, that has been conserved in at least 10 genes encoding subunits of RNA polymerases A and C. ..
- Zinn Justin S, Pillet L, Ducancel F, Thomas A, Smith J, Boulain J, et al. Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody. Protein Eng. 1994;7:917-23 pubmed..We found that full antigenicity was restored in the previously unrecognized variant. The implications of this finding are discussed. ..
- Couriaud C, Ripoche P, Rousselet G. Cloning and functional characterization of a rat urea transporter: expression in the brain. Biochim Biophys Acta. 1996;1309:197-9 pubmed..When expressed in Xenopus oocytes, the protein induces a 10-fold rise in urea permeability, inhibited by mercurial reagents and phloretin. The rUT11 mRNA is massively expressed in the brain. ..
- Jackson C, Casanova J. Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors. Trends Cell Biol. 2000;10:60-7 pubmed..A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals. ..
- Galat A. Sequence diversification of the FK506-binding proteins in several different genomes. Eur J Biochem. 2000;267:4945-59 pubmed..These variations at the sequence positions which are crucial for binding the immunosuppressive macrolide FK506 and peptidyl-prolyl cis/trans isomerase (PPIase) activity are small. ..
- Rouget C, Quinton L, Maïga A, Gales C, Masuyer G, Malosse C, et al. Identification of a novel snake peptide toxin displaying high affinity and antagonist behaviour for the ?2-adrenoceptors. Br J Pharmacol. 2010;161:1361-74 pubmed publisher..Additionally, its affinity and atypical mode of interaction open up the possibility of its use as a new pharmacological tool, in the study of the physiological roles of ?(2)-adrenoceptor subtypes...
- Chiannilkulchai N, Stalder R, Riva M, Carles C, Werner M, Sentenac A. RPC82 encodes the highly conserved, third-largest subunit of RNA polymerase C (III) from Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:4433-40 pubmed..A group of RNA polymerase C-specific subunits are proposed to form a substructure of the enzyme. ..
- Lazard M, Mirande M. Cloning and analysis of a cDNA encoding mammalian arginyl-tRNA synthetase, a component of the multisynthetase complex with a hydrophobic N-terminal extension. Gene. 1993;132:237-45 pubmed..By using the isolated cDNA, a Northern blot analysis showed a single mRNA species. Thus, there is a possibility that the free and complexed forms of ArgRS are encoded by the same gene. ..
- Baudin Baillieu A, Tollervey D, Cullin C, Lacroute F. Functional analysis of Rrp7p, an essential yeast protein involved in pre-rRNA processing and ribosome assembly. Mol Cell Biol. 1997;17:5023-32 pubmed..We believe that Rrp7p is required for correct assembly of rpS27 into the preribosomal particle, with the inhibition of pre-rRNA processing appearing as a consequence of this defect. ..
- Corbi J, Dutheil J, Damerval C, Tenaillon M, Manicacci D. Accelerated evolution and coevolution drove the evolutionary history of AGPase sub-units during angiosperm radiation. Ann Bot. 2012;109:693-708 pubmed publisher..Finally, the pattern we observed during LSU evolution is consistent with repeated sub-functionalization under 'Escape from Adaptive Conflict', a model rarely illustrated in the literature. ..
- Geffrotin C, Chardon P, de Andres Cara D, Feil R, Renard C, Vaiman M. The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex. Anim Genet. 1990;21:1-13 pubmed..The results demonstrate that the swine 21-hydroxylase gene is located within or close to the swine MHC. Taken together, the present results suggest the existence of a single 21-hydroxylase gene per haploid genome. ..
- Wolff N, Gilquin B, Courchay K, Callebaut I, Worman H, Zinn Justin S. Structural analysis of emerin, an inner nuclear membrane protein mutated in X-linked Emery-Dreifuss muscular dystrophy. FEBS Lett. 2001;501:171-6 pubmed..The existence of a conserved solvent-exposed surface on the LEM domains of LAP2 and emerin is discussed, as well as the nature of a possible common target. ..
- Petitjean A, Bonneaud N, Lacroute F. The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins. Mol Cell Biol. 1995;15:5071-81 pubmed..Polysome profile analysis suggests that the primary defect caused by the depletion in Ssm1p is at the level of translation initiation. ..
- Dezeure F, Vaiman M, Chardon P. Characterization of a polymorphic heat shock protein 70 gene in swine outside the SLA major histocompatibility complex. Biochim Biophys Acta. 1993;1174:17-26 pubmed..Restriction mapping of the Hsp70 gene indicated the absence of introns, and showed that the polymorphic PstI site was located upstream of the gene's 5' coding region, and the PvuII site inside its coding region. ..
- Dassa J, Fsihi H, Marck C, Dion M, Kieffer Bontemps M, Boquet P. A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA). Mol Gen Genet. 1991;229:341-52 pubmed
- Mirande M, Waller J. Molecular cloning and primary structure of cDNA encoding the catalytic domain of rat liver aspartyl-tRNA synthetase. J Biol Chem. 1989;264:842-7 pubmed..The primary structure of the DRS1 gene product is discussed in relation to the occurrence of two distinct forms of that enzyme. ..
- Szczepanek T, Lazowska J. Replacement of two non-adjacent amino acids in the S.cerevisiae bi2 intron-encoded RNA maturase is sufficient to gain a homing-endonuclease activity. EMBO J. 1996;15:3758-67 pubmed..capensis bi2-encoded protein which functions in both splicing and intron mobility in the wild-type cells. These results provide new insight into our understanding of the activity and the evolution of group I intron-encoded proteins. ..
- Lazard M, Agou F, Cavarelli J, Latreille M, Moras D, Mirande M. Genomic organization of the rat aspartyl-tRNA synthetase gene family: a single active gene and several retropseudogenes. Gene. 1996;180:197-205 pubmed..Taken together, these results clearly demonstrate that the AspRS gene family is composed of only one active gene. The availability of the gene structure of AspRS could help to clarify molecular evolution of class II aaRS. ..
- Mével Ninio M, Fouilloux E, Guenal I, Vincent A. The three dominant female-sterile mutations of the Drosophila ovo gene are point mutations that create new translation-initiator AUG codons. Development. 1996;122:4131-8 pubmed..We propose that premature expression of long Ovo protein isoforms occurs in ovoD mutants and interferes with wild-type Ovo function in controlling female germline differentiation. ..
- Firsov D, Elalouf J. Molecular cloning of two rat GRK6 splice variants. Am J Physiol. 1997;273:C953-61 pubmed..The identification of GRK6b extends the family of GRKs. Further studies will be required to establish the tissue and subcellular distribution of this protein and to delineate its physiological role...
- Hamel P, Sakamoto W, Wintz H, Dujardin G. Functional complementation of an oxa1- yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J. 1997;12:1319-27 pubmed..Our results suggest that the Oxa1At protein is essential for the respiratory complex assembly in A. thaliana, and that genes involved in mitochondrial multiprotein complex formation can be conserved between plants and other organisms. ..
- Francin M, Kaminska M, Kerjan P, Mirande M. The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain. J Biol Chem. 2002;277:1762-9 pubmed publisher..Our results provide new insights into tRNA(Lys) channeling in eukaryotic cells and shed new light on the possible requirement of native LysRS for triggering tRNA(3)Lys packaging into human immunodeficiency virus, type 1 viral particles...
- Kevei Z, Vinardell J, Kiss G, Kondorosi A, Kondorosi E. Glycine-rich proteins encoded by a nodule-specific gene family are implicated in different stages of symbiotic nodule development in Medicago spp. Mol Plant Microbe Interact. 2002;15:922-31 pubmed..Differences in kinetics and localization of gene activation as well as in the primary structure of proteins suggest nonredundant roles for these GRPs in nodule organogenesis...
- Gaisne M, Bonnefoy N. The COX18 gene, involved in mitochondrial biogenesis, is functionally conserved and tightly regulated in humans and fission yeast. FEMS Yeast Res. 2006;6:869-82 pubmed..This transcriptional control contrasts with the constitutive abundance of the OXA1 mRNAs and might reflect major functional differences between these nevertheless structurally related genes. ..
- Lestini R, Michel B. UvrD controls the access of recombination proteins to blocked replication forks. EMBO J. 2007;26:3804-14 pubmed..UvrD action on RecA is conserved in evolution as it can be performed in E. coli by the UvrD homologue from Bacillus subtilis, PcrA. ..
- James L, Sargueil B. RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region. Nucleic Acids Res. 2008;36:4653-66 pubmed publisher..In addition, we observed footprints of Gag protein on each side of the initiation codon, this sheds light on the role of the sequences required for encapsidation...
- Thomas D, Cherest H, Surdin Kerjan Y. Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur. EMBO J. 1991;10:547-53 pubmed..That AdoMet controls a part of the pentose phosphate pathway sheds new light on mechanisms regulating the relative fluxes of carbon utilization through the pentose phosphate pathway and glycolysis. ..
- Pradel E, Marck C, Boquet P. Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase. J Bacteriol. 1990;172:802-7 pubmed..Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit. ..
- Pompon D. cDNA cloning and functional expression in yeast Saccharomyces cerevisiae of beta-naphthoflavone-induced rabbit liver P-450 LM4 and LM6. Eur J Biochem. 1988;177:285-93 pubmed..Enzymatic and evolutionary implications of these results are discussed. ..
- Deana A, Ehrlich R, Reiss C. Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genes. J Bacteriol. 1996;178:2718-20 pubmed..For the bla gene, the exchange of 24 codons for the most frequently used synonymous codons extended the bla mRNA half-life. A modification of ribosome traffic could account for these observations. ..
- Leriche C, Badet Denisot M, Badet B. Affinity labeling of Escherichia coli glucosamine-6-phosphate synthase with a fructose 6-phosphate analog--evidence for proximity between the N-terminal cysteine and the fructose-6-phosphate-binding site. Eur J Biochem. 1997;245:418-22 pubmed..These results provide evidence for the close proximity of glutamine and fructose-6-phosphate-binding sites recently suggested by Bearne [Bearne, S. L. (1996) J. Biol. Chem. 271, 3052-3057]. ..
- Guigueno A, Dassa J, Belin P, Boquet P. Oversynthesis of a new Escherichia coli small RNA suppresses export toxicity of DsbA'-PhoA unfoldable periplasmic proteins. J Bacteriol. 2001;183:1147-58 pubmed..UptR RNA is the first example of a small RNA implicated in extracytoplasmic toxicity suppression. It appears to offer a new way of suppressing toxicity, and its possible modes of action are discussed. ..
- Sisso ff L, Mousli M, England P, Tuffereau C. Stable trimerization of recombinant rabies virus glycoprotein ectodomain is required for interaction with the p75NTR receptor. J Gen Virol. 2005;86:2543-52 pubmed publisher..Altogether, these results show that the C-terminal fusion of the RVG ectodomain with the fibritin foldon is a powerful way to obtain a recombinant trimeric native-like structure of the p75NTR binding domain of RVG...
- Mosrin C, Riva M, Beltrame M, Cassar E, Sentenac A, Thuriaux P. The RPC31 gene of Saccharomyces cerevisiae encodes a subunit of RNA polymerase C (III) with an acidic tail. Mol Cell Biol. 1990;10:4737-43 pubmed..Its reduced rate of tRNA synthesis in vivo relative to 5.8S rRNA supports the hypothesis that the C31 protein is a functional subunit of RNA polymerase C. ..
- Auzat I, Byrnes W, Garel J, Chang S. Role of residue 161 in the allosteric transitions of two bacterial phosphofructokinases. Biochemistry. 1995;34:7062-8 pubmed
- Cherel I, Thuriaux P. The IFH1 gene product interacts with a fork head protein in Saccharomyces cerevisiae. Yeast. 1995;11:261-70 pubmed..These data suggest a direct interaction between the Fhl1p and Ifh1p proteins, and are consistent with a model where Fhl1p is converted from a transcriptional repressor to an activator on binding of Ifh1p. ..
- Castaing B, Geiger A, Seliger H, Nehls P, Laval J, Zelwer C, et al. Cleavage and binding of a DNA fragment containing a single 8-oxoguanine by wild type and mutant FPG proteins. Nucleic Acids Res. 1993;21:2899-905 pubmed..In contrast, the 4 Fpg proteins with Cys-->Gly mutations within the zinc finger motif neither cleave nor bind the (*G/C) duplex, nor do these proteins suppress the fpg-1 mutator phenotype. ..
- Zahaf O, Blanchet S, de Z licourt A, Alunni B, Plet J, Laffont C, et al. Comparative transcriptomic analysis of salt adaptation in roots of contrasting Medicago truncatula genotypes. Mol Plant. 2012;5:1068-81 pubmed publisher..Despite the global complexity of the differential transcriptional responses, we propose that an increase in this bHLH TF expression may be linked to the adaptation of M. truncatula to saline soil environments...
- Tremeau O, Lemaire C, Drevet P, Pinkasfeld S, Ducancel F, Boulain J, et al. Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues. J Biol Chem. 1995;270:9362-9 pubmed..The variable residues might contribute to the selectivity of Ea for some AcChoRs, including those from fish, the prey of sea snakes. ..
- Mazzoni C, Zarov P, Rambourg A, Mann C. The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae. J Cell Biol. 1993;123:1821-33 pubmed..Furthermore, slt2::HIS3 act1-1 and slt2::HIS3 myo2-66 double mutants are inviable. We suggest that Slt2p functions downstream or in parallel with Cdc28p in promoting bud formation and apical growth. ..