Genomes and Genes
Experts and Doctors on mitochondria in Gif sur Yvette, Île de France, France
Locale: Gif sur Yvette, Île de France, France
- Rouillard J, Brendolise C, Lacroute F. Rna14p, a component of the yeast nuclear cleavage/polyadenylation factor I, is also localised in mitochondria. Mol Gen Genet. 2000;262:1103-12 pubmed..The localisation data strongly suggest that, besides an essential function in mRNA polyadenylation, the Rna14p protein has a non essential function in mitochondrial metabolism. ..
- Bernard D, Gabilly S, Dujardin G, Merchant S, Hamel P. Overlapping specificities of the mitochondrial cytochrome c and c1 heme lyases. J Biol Chem. 2003;278:49732-42 pubmed..Based on the functional interaction of Cyc2p with CCHL and the presence of a putative FAD-binding site in the protein, we hypothesize that Cyc2p controls the redox chemistry of the heme lyase reaction. ..
- Ostojic J, Panozzo C, Lasserre J, Nouet C, Courtin F, Blancard C, et al. The energetic state of mitochondria modulates complex III biogenesis through the ATP-dependent activity of Bcs1. Cell Metab. 2013;18:567-77 pubmed publisher..Our study further highlights the intramitochondrial adenine nucleotide pool as a potential target for the treatment of Bcs1-based disorders. ..
- Kaminska M, Francin M, Shalak V, Mirande M. Role of HIV-1 Vpr-induced apoptosis on the release of mitochondrial lysyl-tRNA synthetase. FEBS Lett. 2007;581:3105-10 pubmed..These results suggest that HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial LysRS. ..
- Gargouri A. The reverse transcriptase encoded by ai1 intron is active in trans in the retro-deletion of yeast mitochondrial introns. FEMS Yeast Res. 2005;5:813-22 pubmed..We conclude that the reverse transcriptase activity, even when encoded by only ai1 intron, can act in trans in the intron deletion process, during the reversion of intronic mutations. ..
- Chiron S, Suleau A, Bonnefoy N. Mitochondrial translation: elongation factor tu is essential in fission yeast and depends on an exchange factor conserved in humans but not in budding yeast. Genetics. 2005;169:1891-901 pubmed..We find that S. cerevisiae EF-Tu is functionally equivalent to the S. pombe EF-Tu/EF-Ts couple. Point mutations in the S. pombe EF-Tu can render it independent of its exchange factor, thereby mimicking the situation in S. cerevisiae. ..
- Harington A, Schwarz E, Slonimski P, Herbert C. Subcellular relocalization of a long-chain fatty acid CoA ligase by a suppressor mutation alleviates a respiration deficiency in Saccharomyces cerevisiae. EMBO J. 1994;13:5531-8 pubmed..These results support the hypothesis that some form of fatty acid synthesis, specific for the mitochondria, is essential for the function of the organelle. ..
- Jia Y, Becam A, Herbert C. The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase. Mol Microbiol. 1997;24:53-9 pubmed..In vitro import experiments showed that cit3p is transported into the mitochondria. Taken together, these data show that the CIT3 gene encodes a second mitochondrial isoform of citrate synthase. ..
- Mirande M. The ins and outs of tRNA transport. EMBO Rep. 2007;8:547-9 pubmed
- Zagorski W, Castaing B, Herbert C, Labouesse M, Martin R, Slonimski P. Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase. J Biol Chem. 1991;266:2537-41 pubmed..The N-terminal sequence of the protein has been determined and shows that the first nine amino acids predicted by the gene sequence have been removed, probably during transport into the mitochondria. ..
- Meunier B, Maréchal A, Rich P. Construction of histidine-tagged yeast mitochondrial cytochrome c oxidase for facile purification of mutant forms. Biochem J. 2012;444:199-204 pubmed publisher..Mutations in both the D and the H channels affect respiratory growth and these effects are discussed in terms of their putative roles in CcO mechanism. ..
- Glatigny A, Mathieu L, Herbert C, Dujardin G, Meunier B, Mucchielli Giorgi M. An in silico approach combined with in vivo experiments enables the identification of a new protein whose overexpression can compensate for specific respiratory defects in Saccharomyces cerevisiae. BMC Syst Biol. 2011;5:173 pubmed publisher..Thus, this systems biology approach appears to be a fruitful new way to study the biogenesis of mitochondrial multi-subunit complexes. ..
- Valerio M, Haraux F. Catalytic and activating protons follow different pathways in the H(+)-ATPase of potato tuber mitochondria. FEBS Lett. 1993;336:83-6 pubmed..The results support the idea of separate pathways or binding sites for catalytic and activating protons. ..
- Groudinsky O, Bousquet I, Wallis M, Slonimski P, Dujardin G. The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae. Mol Gen Genet. 1993;240:419-27 pubmed..The abundance of transcripts from five other genes was either slightly (21S rRNA) or not at all (cox2, cox3, atp9 and 15S rRNA) affected by the nam1 inactivation.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Fosset C, Chauveau M, Guillon B, Canal F, Drapier J, Bouton C. RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells. J Biol Chem. 2006;281:25398-406 pubmed..Finally, depletion of m-Nfs1, which conferred on apo-IRP-1 a high affinity for ferritin mRNA, was associated with the down-regulation of the iron storage protein ferritin. ..
- Rouillard J, Dufour M, Theunissen B, Mandart E, Dujardin G, Lacroute F. SLS1, a new Saccharomyces cerevisiae gene involved in mitochondrial metabolism, isolated as a syntheticlethal in association with an SSM4 deletion. Mol Gen Genet. 1996;252:700-8 pubmed..Thus, the SLS1 gene, encodes a mitochondrial integral membrane protein and is paradoxically synlethal in association with a deletion of the SSM4 gene, which encodes an integral nuclear membrane protein. ..
- Bousquet I, Dujardin G, Slonimski P. ABC1, a novel yeast nuclear gene has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex. EMBO J. 1991;10:2023-31 pubmed
- Rieger K, Lazowska J, Pohl T, Slonimski P, Aljinovic G. A novel nuclear gene, CBT1, essential for mitochondrial cytochrome b formation: terminal processing of mRNA and intron dependence. Curr Genet. 1997;32:163-74 pubmed..We propose, that the CBT1 protein is necessary for the correct trimming of the end of cytb pre-mRNA and may be a part of the multi-component complex involved in this process. ..
- Hamel P, Sakamoto W, Wintz H, Dujardin G. Functional complementation of an oxa1- yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J. 1997;12:1319-27 pubmed..Our results suggest that the Oxa1At protein is essential for the respiratory complex assembly in A. thaliana, and that genes involved in mitochondrial multiprotein complex formation can be conserved between plants and other organisms. ..
- Li G, Becam A, Slonimski P, Herbert C. In vitro mutagenesis of the mitochondrial leucyl tRNA synthetase of Saccharomyces cerevisiae shows that the suppressor activity of the mutant proteins is related to the splicing function of the wild-type protein. Mol Gen Genet. 1996;252:667-75 pubmed..These data show that the splicing and suppressor functions are linked and that the suppressor activity of the mutant alleles results from a modification of the wild-type splicing activity. ..
- Sellem C, Begel O, Sainsard Chanet A. Recombinant mitochondrial DNA molecules suggest a template switching ability for group-II-intron reverse transcriptase. Curr Genet. 2000;37:24-8 pubmed..This activity might be involved in a number of mitochondrial rearrangements occurring in degenerative syndromes and in some long-lived mutants...
- Wallis M, Groudinsky O, Slonimski P, Dujardin G. The NAM1 protein (NAM1p), which is selectively required for cox1, cytb and atp6 transcript processing/stabilisation, is located in the yeast mitochondrial matrix. Eur J Biochem. 1994;222:27-32 pubmed
- Hamel P, Saint Georges Y, de Pinto B, Lachacinski N, Altamura N, Dujardin G. Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana. Mol Microbiol. 2004;51:307-17 pubmed..Finally, we demonstrate that Pic2p is a mitochondrial protein and that its steady state level increases at high temperature. We propose that Pic2p is a minor form of mPic which plays a role under specific stress conditions. ..
- Desjardin C, Chat S, Gilles M, Legendre R, Rivière J, Mata X, et al. Involvement of mitochondrial dysfunction and ER-stress in the physiopathology of equine osteochondritis dissecans (OCD). Exp Mol Pathol. 2014;96:328-38 pubmed publisher..In addition, 11 modulated proteins and several candidate mutations located in QTL regions were identified, bringing new insight into the molecular physiopathology and genetic basis of OCD. ..
- Valerio M, Diolez P, Haraux F. Deactivation of F0F1 ATPase in intact plant mitochondria. Effect of pH and inhibitors. Eur J Biochem. 1994;221:1071-8 pubmed..These results, obtained with intact mitochondria, extend previous observations on the isolated enzyme and question the binding of IF1 as a rate-limiting step for ATPase deactivation. ..
- Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed..The possible mechanisms by which the NAM1 gene product may function are discussed. ..
- Labouesse M. The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns. Mol Gen Genet. 1990;224:209-21 pubmed..We conclude that the mLRS is a splicing factor essential for the excision of the group I introns bI4 and aI4 and probably important for the excision of other group I introns. ..