Genomes and Genes
Experts and Doctors on escherichia coli proteins in Paris, Île de France, France
Locale: Paris, Île de France, France
Topic: escherichia coli proteins
Publications161 found, 100 shown here
- Carlier L, Haase A, Burgos Zepeda M, Dassa E, Lequin O. The C-terminal domain of the Uup protein is a DNA-binding coiled coil motif. J Struct Biol. 2012;180:577-84 pubmed publisher..These structural data are the first reported for a non-ATPase domain within ABC REG subfamily...
- Egile C, d Hauteville H, Parsot C, Sansonetti P. SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneri. Mol Microbiol. 1997;23:1063-73 pubmed..The construction and phenotypic characterization of a sopA mutant demonstrated that SopA is required for exclusive polar localization of IcsA on the bacterial surface and proper expression of the motility phenotype in infected cells...
- Fontaine F, Stewart E, Lindner A, Taddei F. Mutations in two global regulators lower individual mortality in Escherichia coli. Mol Microbiol. 2008;67:2-14 pubmed..The identification of these two mutants implies that reducing mortality is not impossible; rather, due to its cost, it is subject to trade-offs with other traits that contribute to the competitive success of the organism. ..
- Haentjens Sitri J, Allemand F, Springer M, Chiaruttini C. A competition mechanism regulates the translation of the Escherichia coli operon encoding ribosomal proteins L35 and L20. J Mol Biol. 2008;375:612-25 pubmed..We show that the pseudoknot, which is known to be essential for L20 binding and regulation, also enhances 30S binding to mRNA as if this structure is specifically recognised by the ribosome. ..
- Mora L, Klepsch M, Buckingham R, Heurgué Hamard V, Kervestin S, de Zamaroczy M. Dual roles of the central domain of colicin D tRNase in TonB-mediated import and in immunity. J Biol Chem. 2008;283:4993-5003 pubmed..Klebicin D has, like colicin D, a ribonuclease activity toward tRNAArg and a central domain, which can form a complex with ImmD but which does not function in TonB-mediated transport. ..
- Al Refaii A, Alix J. Inhibition of chaperone-dependent bacterial ribosome biogenesis. Methods Mol Med. 2008;142:75-85 pubmed publisher..This question is important due to their implication in many pathways in immunology, cancer biology, and neurodegenerative disorders. ..
- Magnet S, Dubost L, Marie A, Arthur M, Gutmann L. Identification of the L,D-transpeptidases for peptidoglycan cross-linking in Escherichia coli. J Bacteriol. 2008;190:4782-5 pubmed publisher..This activity partially replaces the D,D-transpeptidase activity of penicillin-binding proteins. ..
- Nogueira T, Rankin D, Touchon M, Taddei F, Brown S, Rocha E. Horizontal gene transfer of the secretome drives the evolution of bacterial cooperation and virulence. Curr Biol. 2009;19:1683-91 pubmed publisher..As a consequence, horizontal transfer promoted by agents such as plasmids, phages, or integrons drives microbial cooperation. ..
- Maes A, Gracia C, Bréchemier D, Hamman P, Chatre E, Lemelle L, et al. Role of polyadenylation in regulation of the flagella cascade and motility in Escherichia coli. Biochimie. 2013;95:410-8 pubmed publisher..We propose that PAP I participates in the ability of the bacteria to adapt to and survive detrimental conditions by constantly monitoring and adjusting to its environment. ..
- Plumbridge J, Kolb A. DNA loop formation between Nag repressor molecules bound to its two operator sites is necessary for repression of the nag regulon of Escherichia coli in vivo. Mol Microbiol. 1993;10:973-81 pubmed..These observations show that repression is the result of co-operative binding of the repressor to its two sites, resulting in the formation of a loop of DNA. ..
- Laurent Winter C, Ngo S, Danchin A, Bertin P. Role of Escherichia coli histone-like nucleoid-structuring protein in bacterial metabolism and stress response--identification of targets by two-dimensional electrophoresis. Eur J Biochem. 1997;244:767-73 pubmed..Finally, synthesis of several proteins belonging to the heat-shock regulon, more particularly molecular chaperones, was induced in an hns mutant. ..
- Danchin A. Comparison between the Escherichia coli and Bacillus subtilis genomes suggests that a major function of polynucleotide phosphorylase is to synthesize CDP. DNA Res. 1997;4:9-18 pubmed..This article briefly discusses the availability of cytosine nucleotides in eukaryotes, and suggests that they are derived from phospholipids turnover. Finally, the GC content of genomes is discussed in this new light. ..
- Boursaux Eude C, Margarita D, Belfaiza J, Old I, Saints Girons I. Homologues of helicase genes priA and ruvAB of Borrelia burgdorferi, the Lyme borreliosis agent. Res Microbiol. 1998;149:235-45 pubmed..priA, which encodes the potential recognition factor for the primosome assembly site, was found at 15 kbp from the left telomere. RuvB and PriA sequences have motifs characteristic of helicases: a DExH box and an ATP binding site...
- Seydel A, Gounon P, Pugsley A. Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection. Mol Microbiol. 1999;34:810-21 pubmed..Four representative class II mutants analysed were shown not to carry mutations in the lolA or lolB genes, known to be involved in the sorting of lipoproteins to the outer membrane...
- Giraud A, Matic I, Tenaillon O, Clara A, Radman M, Fons M, et al. Costs and benefits of high mutation rates: adaptive evolution of bacteria in the mouse gut. Science. 2001;291:2606-8 pubmed..The short-term advantages and long-term disadvantages of mutator bacteria could account for their frequency in nature. ..
- Guillier M, Allemand F, Raibaud S, Dardel F, Springer M, Chiaruttini C. Translational feedback regulation of the gene for L35 in Escherichia coli requires binding of ribosomal protein L20 to two sites in its leader mRNA: a possible case of ribosomal RNA-messenger RNA molecular mimicry. RNA. 2002;8:878-89 pubmed..However L20 can bind to each site independently in vitro. One site is similar to the L20-binding site on 23S rRNA. Here we propose that L20 recognizes its mRNA and its rRNA in similar way. ..
- Oberto J, Nabti S, Jooste V, Mignot H, Rouviere Yaniv J. The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction. PLoS ONE. 2009;4:e4367 pubmed publisher..The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism. ..
- Le H, Gautier V, Kthiri F, Kohiyama M, Katayama T, Richarme G. DNA replication defects in a mutant deficient in the thioredoxin homolog YbbN. Biochem Biophys Res Commun. 2011;405:52-7 pubmed publisher..These results suggest that the DNA replication and cell division defects of the ybbN mutant might best be explained by chaperone functions of YbbN in the biogenesis of DNA polymerase 3 holoenzyme. ..
- Bountra K, Hagelueken G, Choudhury H, Corradi V, El Omari K, Wagner A, et al. Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD. EMBO J. 2017;36:3062-3079 pubmed publisher..We propose that shielding of the cavity ensures that the transporter is available to export the newly synthesized peptides, preventing toxic-level build-up. ..
- Plumbridge J. A dominant mutation in the gene for the Nag repressor of Escherichia coli that renders the nag regulon uninducible. J Gen Microbiol. 1992;138:1011-7 pubmed..The mutation is semi-dominant since heterozygous diploids have reduced growth rates on glucosamine and N-acetylglucosamine compared to the wild-type strain. ..
- Raibaud O, Vidal Ingigliardi D, Kolb A. Genetic studies on the promoter of malT, the gene that encodes the activator of the Escherichia coli maltose regulon. Res Microbiol. 1991;142:937-42 pubmed..5, and that all of the elements necessary and sufficient for the regulation by CRP are located downstream from position -122. ..
- Honore N, Cole S. Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins. Nucleic Acids Res. 1990;18:653 pubmed
- Raibaud O, Vidal Ingigliardi D, Richet E. A complex nucleoprotein structure involved in activation of transcription of two divergent Escherichia coli promoters. J Mol Biol. 1989;205:471-85 pubmed..We propose a model for the nucleoprotein structure that might be involved in transcription activation at these divergent promoters. ..
- Dumay V, Danchin A, Crasnier M. Regulation of Escherichia coli adenylate cyclase activity during hexose phosphate transport. Microbiology. 1996;142 ( Pt 3):575-83 pubmed..We present data indicating that Glc6P per se or its degradation is unlikely to be responsible for the low cAMP level. It is concluded that the level of cAMP in the cell is determined by the flux of Glc6P through UhpT. ..
- Arluison V, Folichon M, Marco S, Derreumaux P, Pellegrini O, Seguin J, et al. The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer. Eur J Biochem. 2004;271:1258-65 pubmed..On the basis of these results, we propose that the C-terminal domain could protect the interface between the subunits and stabilize the hexameric Hfq structure. The origin of this C-terminal domain is also discussed. ..
- Alvarez Añorve L, Calcagno M, Plumbridge J. Why does Escherichia coli grow more slowly on glucosamine than on N-acetylglucosamine? Effects of enzyme levels and allosteric activation of GlcN6P deaminase (NagB) on growth rates. J Bacteriol. 2005;187:2974-82 pubmed..The effects of the mutations on growth in vivo are discussed in light of their in vitro kinetics. ..
- Zidi Yahiaoui N, Ripoche P, Le Van Kim C, Gane P, D Ambrosio A, Cartron J, et al. Ammonium transport properties of HEK293 cells expressing RhCG mutants: preliminary analysis of structure/function by site-directed mutagenesis. Transfus Clin Biol. 2006;13:128-31 pubmed
- Zidi Yahiaoui N, Callebaut I, Genetet S, Le Van Kim C, Cartron J, Colin Y, et al. Functional analysis of human RhCG: comparison with E. coli ammonium transporter reveals similarities in the pore and differences in the vestibule. Am J Physiol Cell Physiol. 2009;297:C537-47 pubmed publisher..The impact of the mutations suggests that, although having a common function and a similar channel structure, bacterial AmtB and human Rh vary in several aspects of the NH(3) transport mechanisms. ..
- Warren H, Fitting C, Hoff E, Adib Conquy M, Beasley Topliffe L, Tesini B, et al. Resilience to bacterial infection: difference between species could be due to proteins in serum. J Infect Dis. 2010;201:223-32 pubmed publisher..The involvement of circulating proteins as key molecules raises hope that the process might be manipulated to create better animal models and potentially new drug targets. ..
- Korea C, Ghigo J, Beloin C. The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism. Bioessays. 2011;33:300-11 pubmed publisher
- Maes A, Gracia C, Hajnsdorf E, Regnier P. Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs. Mol Microbiol. 2012;83:436-51 pubmed publisher..This demonstrates that the surveillance of tRNA maturation described for a defective tRNA also applies to a wild-type tRNA. ..
- Bonis M, Williams A, Guadagnini S, Werts C, Boneca I. The effect of bulgecin A on peptidoglycan metabolism and physiology of Helicobacter pylori. Microb Drug Resist. 2012;18:230-9 pubmed publisher..Hence, we propose that bulgecin A constitutes an attractive compound for the development of new therapeutic strategies against H. pylori combined with other inhibitors of PG biosynthetic enzymes. ..
- Dezi M, Di Cicco A, Bassereau P, Levy D. Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents. Proc Natl Acad Sci U S A. 2013;110:7276-81 pubmed publisher..Our method should thus apply to a wide variety of membrane or peripheral proteins for producing more complex biomimetic GUVs...
- Maes A, Gracia C, Innocenti N, Zhang K, Aurell E, Hajnsdorf E. Landscape of RNA polyadenylation in E. coli. Nucleic Acids Res. 2017;45:2746-2756 pubmed publisher..Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments. ..
- Bonnefoy E, Almeida A, Rouviere Yaniv J. Lon-dependent regulation of the DNA binding protein HU in Escherichia coli. Proc Natl Acad Sci U S A. 1989;86:7691-5 pubmed
- Possot O, Vignon G, Bomchil N, Ebel F, Pugsley A. Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE. J Bacteriol. 2000;182:2142-52 pubmed
- Chami M, Pehau Arnaudet G, Lambert O, Ranck J, Levy D, Rigaud J. Use of octyl beta-thioglucopyranoside in two-dimensional crystallization of membrane proteins. J Struct Biol. 2001;133:64-74 pubmed
- Mathy N, Pellegrini O, Serganov A, Patel D, Ehresmann C, Portier C. Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation. Mol Microbiol. 2004;52:661-75 pubmed..However, specific S15-rpsO mRNA interactions can also be found, probably with A(-46) in loop L1 of the pseudoknot, demonstrating that mimicry between the two targets is limited. ..
- Herve G, Schmitt B, Serre V. Cooperativity and high pressure: stabilization of the R conformation of the allosteric aspartate transcarbamylase under the influence of pressure. Cell Mol Biol (Noisy-le-grand). 2004;50:347-52 pubmed..This result emphasizes a significant role of the protein-solvent interactions in this important regulatory conformational change. ..
- Guianvarc h D, Guangqi E -, Drujon T, Rey C, Wang Q, Ploux O. Identification of inhibitors of the E. coli cyclopropane fatty acid synthase from the screening of a chemical library: In vitro and in vivo studies. Biochim Biophys Acta. 2008;1784:1652-8 pubmed publisher..coli cells, at 150 microM. This molecule is thus the first inhibitor of a cyclopropane synthase that is active in vivo, contrary to sinefungin and other analogs that are only active on the isolated enzyme. ..
- Elez M, Radman M, Matic I. Stoichiometry of MutS and MutL at unrepaired mismatches in vivo suggests a mechanism of repair. Nucleic Acids Res. 2012;40:3929-38 pubmed publisher..Our results corroborate the hypothesis postulating that MutL accumulation assures the coordination of the MMR activities between the mismatch and the strand discrimination site. ..
- Turlin E, Debarbouille M, Augustyniak K, Gilles A, Wandersman C. Staphylococcus aureus FepA and FepB proteins drive heme iron utilization in Escherichia coli. PLoS ONE. 2013;8:e56529 pubmed publisher..Our results show that the function in heme iron extraction is conserved in the two orthologous systems. ..
- Choudhury H, Tong Z, Mathavan I, Li Y, Iwata S, Zirah S, et al. Structure of an antibacterial peptide ATP-binding cassette transporter in a novel outward occluded state. Proc Natl Acad Sci U S A. 2014;111:9145-50 pubmed publisher..coli confirmed the presence of the occluded state. We therefore propose that the outward-occluded state represents a transition intermediate between the outward-open and inward-open conformation of ABC exporters. ..
- Vignon G, Köhler R, Larquet E, Giroux S, Prevost M, Roux P, et al. Type IV-like pili formed by the type II secreton: specificity, composition, bundling, polar localization, and surface presentation of peptides. J Bacteriol. 2003;185:3416-28 pubmed..Abnormally long pili that extend beyond the outer membrane are produced only when pilus length control and retraction are deregulated by overproduction of the major pseudopilus subunit (PulG). ..
- Gagyi C, Bucurenci N, Sîrbu O, Labesse G, Ionescu M, Ofiteru A, et al. UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity. Eur J Biochem. 2003;270:3196-204 pubmed..subtilis and E. coli enzymes. ..
- Saleh O, Bigot S, Barre F, Allemand J. Analysis of DNA supercoil induction by FtsK indicates translocation without groove-tracking. Nat Struct Mol Biol. 2005;12:436-40 pubmed..We show that this rate of supercoil induction is markedly near to the ideal value that would minimize in vivo disturbance to the chromosomal supercoil density, suggesting an origin for the unusual rotational behavior of FtsK. ..
- Martineau P, Saurin W, Hofnung M, Spurlino J, Quiocho F. Progress in the identification of interaction sites on the periplasmic maltose binding protein from E coli. Biochimie. 1990;72:397-402 pubmed..Because of the structural similarities between periplasmic binding proteins, the regions of interaction defined could be relevant for other members of this family...
- Hardie K, Lory S, Pugsley A. Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J. 1996;15:978-88 pubmed..PulS is only the second component of the main terminal branch of the GSP for which a precise function can be proposed. ..
- Kuznetsov S, Sidorkina O, Jurado J, Bazin M, Tauc P, Brochon J, et al. Effect of single mutations on the structural dynamics of a DNA repair enzyme, the Escherichia coli formamidopyrimidine-DNA glycosylase--a fluorescence study using tryptophan residues as reporter groups. Eur J Biochem. 1998;253:413-20 pubmed..The influence of the single mutations on the enzyme activities of the Fpg protein is discussed. ..
- Bourhy P, Saint Girons I. Localization of the Leptospira interrogans metF gene on the CII secondary chromosome. FEMS Microbiol Lett. 2000;191:259-63 pubmed..interrogans MetF was found to be thermolabile. The metF gene belonged to the CII secondary chromosome, in contrast to the previously isolated metY and metX genes, which have been localized to the CI chromosome of Leptospira sp. ..
- Plumbridge J. DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli. Nucleic Acids Res. 2001;29:506-14 pubmed..Replacing the A/T at +11/-11 with C/G allows repression by NagC in the absence of the nagB operator. ..
- Plumbridge J. Regulation of PTS gene expression by the homologous transcriptional regulators, Mlc and NagC, in Escherichia coli (or how two similar repressors can behave differently). J Mol Microbiol Biotechnol. 2001;3:371-80 pubmed..Displacement of Mlc from its binding sites occurs during growth on glucose and other PTS sugars and involves sequestration of the repressor to membranes by binding to dephosphorylated PtsG. ..
- Schlecht G, Loucka J, Najar H, Sebo P, Leclerc C. Antigen targeting to CD11b allows efficient presentation of CD4+ and CD8+ T cell epitopes and in vivo Th1-polarized T cell priming. J Immunol. 2004;173:6089-97 pubmed..In vivo, CyaA induces strong and specific Th1 CD4(+) and CD8(+) T cell responses against, respectively, the MalE(100-114) and OVA(257-264) epitopes. These results underscore the potency of CyaA for design of new vaccines...
- Folichon M, Allemand F, Regnier P, Hajnsdorf E. Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails. FEBS J. 2005;272:454-63 pubmed..We demonstrate also that Hfq binding is not affected by the phosphorylation status of the RNA molecule and occurs equally well at terminal or internal stretches of poly(A). ..
- Caldas T, Malki A, Kern R, Abdallah J, Richarme G. The Escherichia coli thioredoxin homolog YbbN/Trxsc is a chaperone and a weak protein oxidoreductase. Biochem Biophys Res Commun. 2006;343:780-6 pubmed
- Houdouin V, Bonacorsi S, Mahjoub Messai F, Mariani Kurkdjian P, Bidet P, Sebag G, et al. Phylogenetic groups and virulence factors of Escherichia coli strains causing pyelonephritis in children with and without urinary tract abnormalities. Clin Microbiol Infect. 2007;13:740-2 pubmed..Further studies are required to determine whether screening for E. coli virulence factors may help to identify children warranting anatomical investigations. ..
- Peigne C, Bidet P, Mahjoub Messai F, Plainvert C, Barbe V, Medigue C, et al. The plasmid of Escherichia coli strain S88 (O45:K1:H7) that causes neonatal meningitis is closely related to avian pathogenic E. coli plasmids and is associated with high-level bacteremia in a neonatal rat meningitis model. Infect Immun. 2009;77:2272-84 pubmed publisher..9 log CFU/ml). Thus, pS88 is a major virulence determinant related to avian pathogenic plasmids that has spread not only through meningitis clonal groups but also human urosepsis and avian pathogenic strains. ..
- Kthiri F, Le H, Gautier V, Caldas T, Malki A, Landoulsi A, et al. Protein aggregation in a mutant deficient in yajL, the bacterial homolog of the Parkinsonism-associated protein DJ-1. J Biol Chem. 2010;285:10328-36 pubmed publisher..These two functions of YajL might explain the protein aggregation phenotype of the yajL mutant. ..
- Karimova G, Davi M, Ladant D. The ?-lactam resistance protein Blr, a small membrane polypeptide, is a component of the Escherichia coli cell division machinery. J Bacteriol. 2012;194:5576-88 pubmed..Altogether, our results suggest that the small membrane polypeptide Blr is a novel component of the E. coli cell division apparatus involved in the stabilization of the divisome under certain stress conditions. ..
- Vimont S, Boyd A, Bleibtreu A, Bens M, Goujon J, Garry L, et al. The CTX-M-15-producing Escherichia coli clone O25b: H4-ST131 has high intestine colonization and urinary tract infection abilities. PLoS ONE. 2012;7:e46547 pubmed publisher..In conclusion, our findings indicate that TN03 is a potent UPEC strain that colonizes the intestinal tract and may persist in the kidneys of infected hosts...
- Coornaert A, Chiaruttini C, Springer M, Guillier M. Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB. PLoS Genet. 2013;9:e1003156 pubmed publisher..These results highlight the complex connections between small RNAs and transcriptional regulation networks in bacteria...
- Mora L, Moncoq K, England P, Oberto J, de Zamaroczy M. The Stable Interaction Between Signal Peptidase LepB of Escherichia coli and Nuclease Bacteriocins Promotes Toxin Entry into the Cytoplasm. J Biol Chem. 2015;290:30783-96 pubmed publisher..Genomic screening identified the conserved LepB binding motif in colicin-like ORFs from 13 additional bacterial species. These findings establish a new paradigm for the functional adaptability of an essential inner-membrane enzyme. ..
- Gaschignard J, Geslain G, Mallet C, Lorrot M, Blot N, Alison M, et al. Spondylodiscitis in a healthy 12-year-old girl with Extraintestinal pathogenic Escherichia coli (ExPEC) bacteraemia. BMC Infect Dis. 2017;17:380 pubmed publisher..coli (ExPEC). Immunological work-up was normal. The identification of E. coli in a spondylodiscitis should lead to the search for immunosuppression of the host and virulence factors of the strain, particularly those of ExPEC. ..
- Rida S, Caillet J, Alix J. Amplification of a novel gene, sanA, abolishes a vancomycin-sensitive defect in Escherichia coli. J Bacteriol. 1996;178:94-102 pubmed..coli chromosome by homologous recombination, is perfectly viable, but after two generations at high temperature (43 degrees C), the barrier function of its envelope towards vancomycin is defective...
- Plumbridge J. How to achieve constitutive expression of a gene within an inducible operon: the example of the nagC gene of Escherichia coli. J Bacteriol. 1996;178:2629-36 pubmed..The nagC gene is subject to low-level translation so that the amount of NagC protein is kept low despite the relatively high transcription levels. ..
- Hajnsdorf E, Regnier P. Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I. Proc Natl Acad Sci U S A. 2000;97:1501-5 pubmed..The similar properties of Hfq and the PABPII poly(A) binding protein, which stimulates poly(A) tail elongation in mammals, indicates that similar mechanisms control poly(A) tail synthesis in prokaryotes and eukaryotes. ..
- Mora L, Heurgué Hamard V, Champ S, Ehrenberg M, Kisselev L, Buckingham R. The essential role of the invariant GGQ motif in the function and stability in vivo of bacterial release factors RF1 and RF2. Mol Microbiol. 2003;47:267-75 pubmed
- Ziolkowska K, Derreumaux P, Folichon M, Pellegrini O, Regnier P, Boni I, et al. Hfq variant with altered RNA binding functions. Nucleic Acids Res. 2006;34:709-20 pubmed
- Kern R, Malki A, Abdallah J, Tagourti J, Richarme G. Escherichia coli HdeB is an acid stress chaperone. J Bacteriol. 2007;189:603-10 pubmed..Thus, we can conclude that Escherichia coli possesses two acid stress chaperones that prevent periplasmic-protein aggregation at acidic pH. ..
- Coornaert A, Lu A, Mandin P, Springer M, Gottesman S, Guillier M. MicA sRNA links the PhoP regulon to cell envelope stress. Mol Microbiol. 2010;76:467-79 pubmed publisher..In addition, we found that Hfq strongly affects the expression of phoP independently of MicA, raising the possibility that even more sRNAs, which remain to be identified, could regulate PhoPQ synthesis. ..
- Clermont O, Gordon D, Brisse S, Walk S, Denamur E. Characterization of the cryptic Escherichia lineages: rapid identification and prevalence. Environ Microbiol. 2011;13:2468-77 pubmed publisher..These results suggest that members of the cryptic clades are unlikely to be of significance to human and health but may influence the use of 'E. coli' as an indicator of water quality. ..
- Skurnik D, Clermont O, Guillard T, Launay A, Danilchanka O, Pons S, et al. Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans. Mol Biol Evol. 2016;33:898-914 pubmed publisher..coli clonal complex of animal origin capable of spreading antimicrobial resistance to other members of microbial communities. ..
- Malabirade A, Jiang K, Kubiak K, Diaz Mendoza A, Liu F, van Kan J, et al. Compaction and condensation of DNA mediated by the C-terminal domain of Hfq. Nucleic Acids Res. 2017;45:7299-7308 pubmed publisher..The propensity for bridging and compaction of DNA by the C-terminal domain might be related to aggregation of bound protein and may have implications for protein binding related gene regulation. ..
- Kaminski P, Desnoues N, Elmerich C. The expression of nifA in Azorhizobium caulinodans requires a gene product homologous to Escherichia coli HF-I, an RNA-binding protein involved in the replication of phage Q beta RNA. Proc Natl Acad Sci U S A. 1994;91:4663-7 pubmed..A null mutant, constructed by disruption of nrfA, exhibited the same phenotype as the missense mutant. Thus, an additional factor can be added to the already complex system of nifA regulation in A. caulinodans...
- Danot O, Raibaud O. On the puzzling arrangement of the asymmetric MalT-binding sites in the MalT-dependent promoters. Proc Natl Acad Sci U S A. 1993;90:10999-1003 pubmed
- Plumbridge J. Control of the expression of the manXYZ operon in Escherichia coli: Mlc is a negative regulator of the mannose PTS. Mol Microbiol. 1998;27:369-80 pubmed..The mlc gene is shown to be allelic with the previously characterized dgsA mutation affecting the mannose phosphoenolpyruvate-dependent phosphotransferase system (PTS). ..
- Plumbridge J. A mutation which affects both the specificity of PtsG sugar transport and the regulation of ptsG expression by Mlc in Escherichia coli. Microbiology. 2000;146 ( Pt 10):2655-63 pubmed..Analysis of the two mutations present individually on plasmids shows that the IIC mutation is responsible for both the effect on sugar specificity and regulation. ..
- Dawid A, Croquette V, Grigoriev M, Heslot F. Single-molecule study of RuvAB-mediated Holliday-junction migration. Proc Natl Acad Sci U S A. 2004;101:11611-6 pubmed..The average migration rate at zero load was found to be approximately 43 bp/sec. Furthermore, the load dependence of the migration rate is small, within the force range of -3.4 pN (hindering force) to +3.4 pN (assisting force). ..
- Destoumieux Garzón D, Duquesne S, Peduzzi J, Goulard C, Desmadril M, Letellier L, et al. The iron-siderophore transporter FhuA is the receptor for the antimicrobial peptide microcin J25: role of the microcin Val11-Pro16 beta-hairpin region in the recognition mechanism. Biochem J. 2005;389:869-76 pubmed..coli. Therefore the Val11-Pro16 beta-hairpin region of MccJ25, which is disrupted upon cleavage by thermolysin, is required for microcin recognition. ..
- Saleh O, Allemand J, Croquette V, Bensimon D. Single-molecule manipulation measurements of DNA transport proteins. Chemphyschem. 2005;6:813-8 pubmed..Each of the experiments described represents a significant advance in the understanding of the mechanisms of DNA transport. ..
- Clermont O, Lavollay M, Vimont S, Deschamps C, Forestier C, Branger C, et al. The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup. J Antimicrob Chemother. 2008;61:1024-8 pubmed publisher..coli diffusing clone is associated with a high level of antibiotic resistance and with high virulence, showing that, under certain selective pressures, the previously observed trade-off between resistance and virulence may not apply...
- El Qaidi S, Plumbridge J. Switching control of expression of ptsG from the Mlc regulon to the NagC regulon. J Bacteriol. 2008;190:4677-86 pubmed publisher..In addition, these experiments confirmed that two operators are necessary for regulation by NagC. We also show that regulation of ptsG by Mlc involves some cooperative binding of Mlc to the two operators. ..
- Low H, Gubellini F, Rivera Calzada A, Braun N, Connery S, Dujeancourt A, et al. Structure of a type IV secretion system. Nature. 2014;508:550-553 pubmed publisher..Our results show a secretion system with markedly different architecture, and consequently mechanism, to other known bacterial secretion systems. ..
- Dardonville B, Raibaud O. Characterization of malT mutants that constitutively activate the maltose regulon of Escherichia coli. J Bacteriol. 1990;172:1846-52 pubmed..Both proteins exhibited a fivefold-higher affinity for maltotriose than the wild-type protein did. ..
- Eiglmeier K, Honore N, Iuchi S, Lin E, Cole S. Molecular genetic analysis of FNR-dependent promoters. Mol Microbiol. 1989;3:869-78 pubmed..Studies with two mutant frd promoters from Escherichia coli, displaying altered regulation and FNR response, provided additional evidence for recognition of this sequence by FNR. ..