Genomes and Genes
Experts and Doctors on base sequence in Gif sur Yvette, Île de France, France
Locale: Gif sur Yvette, Île de France, France
Topic: base sequence
Publications133 found, 100 shown here
- Rousset F, De Stordeur E. Properties of Drosophila simulans strains experimentally infected by different clones of the bacterium Wolbachia. Heredity (Edinb). 1994;72 ( Pt 4):325-31 pubmed
- Castaing B, Geiger A, Seliger H, Nehls P, Laval J, Zelwer C, et al. Cleavage and binding of a DNA fragment containing a single 8-oxoguanine by wild type and mutant FPG proteins. Nucleic Acids Res. 1993;21:2899-905 pubmed..In contrast, the 4 Fpg proteins with Cys-->Gly mutations within the zinc finger motif neither cleave nor bind the (*G/C) duplex, nor do these proteins suppress the fpg-1 mutator phenotype. ..
- Guyot J, Grassi J, Hahn U, Guschlbauer W. The role of the preserved sequences of Dam methylase. Nucleic Acids Res. 1993;21:3183-90 pubmed..We discuss the significance of these results for the understanding of the functional properties of the enzyme. ..
- Potier M, Chelot E, Pekarsky Y, Gardiner K, Rossier J, Turnell W. The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21. Genomics. 1995;29:562-70 pubmed..The chromosomal localization of the gene for human MLCK is shown to be at 3qcen-q21, as determined by PCR and Southern blotting using two somatic cell hybrid panels. ..
- Leung J, Merlot S, Giraudat J. The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell. 1997;9:759-71 pubmed..Thus, the homologous ABI1 and ABI2 phosphatases appear to assume partially redundant functions in ABA signaling, which may provide a mechanism to maintain informational homeostasis. ..
- Da Lage J, Maisonhaute C, Maczkowiak F, Cariou M. A nested alpha-amylase gene in Drosophila ananassae. J Mol Evol. 2003;57:355-62 pubmed publisher..Our data also help to elucidate the history of the Amy family, suggesting that Amy35 arose by duplication and translocation from another ancestral locus, into a formerly short intron, in an ancestor of the subgroup...
- Guigou L, Shalak V, Mirande M. The tRNA-interacting factor p43 associates with mammalian arginyl-tRNA synthetase but does not modify its tRNA aminoacylation properties. Biochemistry. 2004;43:4592-600 pubmed..It promotes association of ArgRS to the complex via its N-terminal domain, but its C-terminal RNA-binding domain may act as a tRNA-interacting factor for an as yet unidentified component of the complex. ..
- D Angelo Picard C, Faure D, Penot I, Dessaux Y. Diversity of N-acyl homoserine lactone-producing and -degrading bacteria in soil and tobacco rhizosphere. Environ Microbiol. 2005;7:1796-808 pubmed
- Das B, Bischerour J, Val M, Barre F. Molecular keys of the tropism of integration of the cholera toxin phage. Proc Natl Acad Sci U S A. 2010;107:4377-82 pubmed publisher..Finally, we showed how the evolution of phage attachment and chromosome dimer resolution sites contributes to the generation of genetic diversity among cholera epidemic strains. ..
- Cormier L, Barbey R, Kuras L. Transcriptional plasticity through differential assembly of a multiprotein activation complex. Nucleic Acids Res. 2010;38:4998-5014 pubmed publisher..We also show evidence suggesting that integration of PDC6 to the Met4 regulon may have occurred recently in the evolution of the Saccharomyces cerevisiae lineage. ..
- Nagy M, Lacroute F, Thomas D. Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc Natl Acad Sci U S A. 1992;89:8966-70 pubmed..cerevisiae uses fumarate as terminal electron acceptor. These results are discussed in relation to the anaerobic growth competence of the two yeasts and to the fermentative processes they use. ..
- Thomas D, Cherest H, Surdin Kerjan Y. Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur. EMBO J. 1991;10:547-53 pubmed..That AdoMet controls a part of the pentose phosphate pathway sheds new light on mechanisms regulating the relative fluxes of carbon utilization through the pentose phosphate pathway and glycolysis. ..
- Bergerat A, Guschlbauer W. The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli. Nucleic Acids Res. 1990;18:4369-75 pubmed
- Dje M, Mazabraud A, Viel A, le Maire M, Denis H, Crawford E, et al. Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis. Nucleic Acids Res. 1990;18:3489-93 pubmed..The latter two proteins are very similar to known eukaryotic EF-1 alpha from other organisms and presumably function in their respective cell types to support protein synthesis. ..
- Thomas D, Surdin Kerjan Y. SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression. J Biol Chem. 1987;262:16704-9 pubmed..The DNA sequence of SAM1 is reported. The translation product shows a high homology with the one deduced from the sequence of the MetK gene encoding the SAM synthetase of Escherichia coli. ..
- Bejanin S, Cervini R, Mallet J, Berrard S. A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine. J Biol Chem. 1994;269:21944-7 pubmed..Two contain the same 5' non-coding region as two ChAT mRNAs and, therefore, are derived from the ChAT transcription unit by alternative splicing. The third mRNA may be transcribed from an additional internal promoter. ..
- Chaminade M, Chelot E, Prado De Carvalho L, Bochet P, Rossier J. Cat proenkephalin-A does not contain the opioid octapeptide. Neurochem Int. 1996;28:155-60 pubmed..Furthermore, the peptide is not flanked by paired basic residues, suggesting it is not processed. ..
- Branny P, de la Torre F, Garel J. The genes for phosphofructokinase and pyruvate kinase of Lactobacillus delbrueckii subsp. bulgaricus constitute an operon. J Bacteriol. 1996;178:4727-30 pubmed..9-kb RNA. The nucleotide sequence of the pyk gene indicated that the encoded protein possessed an extra C-terminal domain with a potential phosphoenolpyruvate-dependent autophosphorylation site. ..
- Rouillard J, Dufour M, Theunissen B, Mandart E, Dujardin G, Lacroute F. SLS1, a new Saccharomyces cerevisiae gene involved in mitochondrial metabolism, isolated as a syntheticlethal in association with an SSM4 deletion. Mol Gen Genet. 1996;252:700-8 pubmed..Thus, the SLS1 gene, encodes a mitochondrial integral membrane protein and is paradoxically synlethal in association with a deletion of the SSM4 gene, which encodes an integral nuclear membrane protein. ..
- Busseau I, Malinsky S, Balakireva M, Chaboissier M, Teninges D, Bucheton A. A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans. Genetics. 1998;148:267-75 pubmed..This represents a promising step toward the use of marked I elements to study retrotransposition and as tools for mutagenesis. ..
- Laurent V, Loisel T, Harbeck B, Wehman A, Gröbe L, Jockusch B, et al. Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J Cell Biol. 1999;144:1245-58 pubmed..We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment-detachment of VASP to F-actin allows its sliding along the growing filament. ..
- Espeli O, Boccard F. Organization of the Escherichia coli chromosome into macrodomains and its possible functional implications. J Struct Biol. 2006;156:304-10 pubmed..The possible involvement of macrodomains in these various processes is discussed. ..
- Marsolier Kergoat M, Yeramian E. GC content and recombination: reassessing the causal effects for the Saccharomyces cerevisiae genome. Genetics. 2009;183:31-8 pubmed publisher..These results pave the way for further analyses aimed at the detailed untangling of drives involved in the evolutionary shaping of the yeast genome. ..
- Marsolier Kergoat M. A simple model for the influence of meiotic conversion tracts on GC content. PLoS ONE. 2011;6:e16109 pubmed publisher..Moreover, the model points out to specific constraints on protein fragments encoded by exon terminal sequences, which are the most affected by the GC bias. ..
- Li C, Costa M, Bassi G, Lai Y, Michel F. Recurrent insertion of 5'-terminal nucleotides and loss of the branchpoint motif in lineages of group II introns inserted in mitochondrial preribosomal RNAs. RNA. 2011;17:1321-35 pubmed publisher..Such coincidences suggest causal relationships between the shift to DNA-based mobility, the loss of one of the two ribozyme sites for binding the 5' exon, and the exclusive use of hydrolysis to initiate splicing. ..
- Mirabeau O, Joly J. Molecular evolution of peptidergic signaling systems in bilaterians. Proc Natl Acad Sci U S A. 2013;110:E2028-37 pubmed publisher..In addition to shedding light on the function of human G protein-coupled receptor PSs, this work presents orthology markers to study ancestral neuron types that were probably present in the last common bilaterian ancestor. ..
- Kondorosi E, Buiré M, Cren M, Iyer N, Hoffmann B, Kondorosi A. Involvement of the syrM and nodD3 genes of Rhizobium meliloti in nod gene activation and in optimal nodulation of the plant host. Mol Microbiol. 1991;5:3035-48 pubmed..nodD3 is regulated by SyrM, NodD1, nodD3, the repressor NoIR, and two putative factors. ..
- Thomas D, Surdin Kerjan Y. Structure of the HOM2 gene of Saccharomyces cerevisiae and regulation of its expression. Mol Gen Genet. 1989;217:149-54 pubmed..We have found that ASA DH is the first reported enzyme of the related threonine and methionine pathway to be regulated by the general control of amino acid synthesis. ..
- Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed..The possible mechanisms by which the NAM1 gene product may function are discussed. ..
- Vaudequin Dransart V, Petit A, Poncet C, Ponsonnet C, Nesme X, Jones J, et al. Novel Ti plasmids in Agrobacterium strains isolated from fig tree and chrysanthemum tumors and their opinelike molecules. Mol Plant Microbe Interact. 1995;8:311-21 pubmed..These results have strong ecological and evolutionary inferences which fit previous speculation on the origin of opine-related functions. ..
- Pelisson A, Song S, Prud homme N, Smith P, Bucheton A, Corces V. Gypsy transposition correlates with the production of a retroviral envelope-like protein under the tissue-specific control of the Drosophila flamenco gene. EMBO J. 1994;13:4401-11 pubmed..We propose a model suggesting that gypsy germinal transposition might occur only in individuals that have maternally inherited enveloped gypsy particles due to infection of the maternal germline by the soma. ..
- Abitbol M, Menini C, Delezoide A, Rhyner T, Vekemans M, Mallet J. Nucleus basalis magnocellularis and hippocampus are the major sites of FMR-1 expression in the human fetal brain. Nat Genet. 1993;4:147-53 pubmed..The early transcription of FMR-1 gene and the distribution of FMR-1 mRNAs in human fetuses suggest that alterations of FMR-1 gene expression may contribute to the pathogenesis of fragile-X syndrome and especially the mental retardation. ..
- Bensmihen S, Rippa S, Lambert G, Jublot D, Pautot V, Granier F, et al. The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis. Plant Cell. 2002;14:1391-403 pubmed..This study illustrates how two homologous transcription factors can play antagonistic roles to fine-tune gene expression. ..
- Delaunay A, Pflieger D, Barrault M, Vinh J, Toledano M. A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation. Cell. 2002;111:471-81 pubmed..These data reveal a redox-signaling function for a GPx-like enzyme and elucidate a eukaryotic hydroperoxide-sensing mechanism. Gpx3 is thus a hydroperoxide receptor and redox-transducer. ..
- Marck C, Grosjean H. Identification of BHB splicing motifs in intron-containing tRNAs from 18 archaea: evolutionary implications. RNA. 2003;9:1516-31 pubmed
- Chardon F, Damerval C. Phylogenomic analysis of the PEBP gene family in cereals. J Mol Evol. 2005;61:579-90 pubmed..A phylogenomic approach leads to some hypotheses about conservation of gene function within the subfamilies. ..
- Montchamp Moreau C, Ogereau D, Chaminade N, Colard A, Aulard S. Organization of the sex-ratio meiotic drive region in Drosophila simulans. Genetics. 2006;174:1365-71 pubmed publisher..In situ hybridization to polytene chromosomes revealed a complete association of the duplication with the sex-ratio trait in random samples of X chromosomes from Madagascar and Reunion...
- Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site. J Biol Chem. 2006;281:37159-74 pubmed..Hence, the main role of SR proteins at site A3 is to counteract hnRNP A1 binding on ESS2 and ESE2. Indeed, we found that ESE2 has inhibitory properties because of its ability to bind hnRNP A1. ..
- Figueroa Bossi N, Valentini M, Malleret L, Fiorini F, Bossi L. Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target. Genes Dev. 2009;23:2004-15 pubmed publisher..Overall, our findings blur the distinction between effector and target in sRNA regulation, raising the possibility that some of the currently defined targets could actually be inhibitors of sRNA function. ..
- Kano S, Xiao J, Osorio J, Ekker M, Hadzhiev Y, Muller F, et al. Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs. PLoS ONE. 2010;5:e13332 pubmed publisher..In addition, they show some lamprey-specific features, including duplication and structural (but not functional) changes in the intronic/regulatory sequences. ..
- Hinaux H, Poulain J, Da Silva C, Noirot C, Jeffery W, Casane D, et al. De novo sequencing of Astyanax mexicanus surface fish and Pachón cavefish transcriptomes reveals enrichment of mutations in cavefish putative eye genes. PLoS ONE. 2013;8:e53553 pubmed publisher..Based on these sequence differences, we provide a list of 11 genes that are potential candidates for having a role in cavefish visual system degeneration...
- Kamoun C, Payen T, Hua Van A, Filee J. Improving prokaryotic transposable elements identification using a combination of de novo and profile HMM methods. BMC Genomics. 2013;14:700 pubmed publisher..For metagenomic data, profile HMM search should be favored, a BLAST-based step is only useful to the final annotation into groups and families. ..
- Rousset F, Vautrin D, Solignac M. Molecular identification of Wolbachia, the agent of cytoplasmic incompatibility in Drosophila simulans, and variability in relation with host mitochondrial types. Proc Biol Sci. 1992;247:163-8 pubmed..There is no direct relation between bacterial phylogeny and formerly identified incompatibility types. ..
- Liljelund P, Mariotte S, Buhler J, Sentenac A. Characterization and mutagenesis of the gene encoding the A49 subunit of RNA polymerase A in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1992;89:9302-5 pubmed..The truncated and wild-type subunits compete equally for assembly in the heterozygous diploid, although the wild type is phenotypically dominant. ..
- Kheyr Pour A, Bendahmane M, Matzeit V, Accotto G, Crespi S, Gronenborn B. Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Res. 1991;19:6763-9 pubmed..Contrary to the other whitefly-transmitted geminiviruses described so far, there is no evidence for the existence nor the necessity of a second component (B DNA) in the TYLCV-S genome...
- Branny P, de la Torre F, Garel J. Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus. J Bacteriol. 1993;175:5344-9 pubmed..bulgaricus, the coordinated regulation of phosphofructokinase and pyruvate kinase occurs at the levels of both biosynthesis and enzymatic activity...
- James L, Sargueil B. RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region. Nucleic Acids Res. 2008;36:4653-66 pubmed publisher..In addition, we observed footprints of Gag protein on each side of the initiation codon, this sheds light on the role of the sequences required for encapsidation...
- Jézéquel L, Loeper J, Pompon D. Sequence-independent construction of ordered combinatorial libraries with predefined crossover points. Biotechniques. 2008;45:523-32 pubmed publisher..Fragments are finally reassembled using a fusion PCR. The process is illustrated via the construction of a set of mosaic CYP2B enzymes using this highly modular approach. ..
- Terzian C, Laprevotte I, Brouillet S, Henaut A. Genomic signatures: tracing the origin of retroelements at the nucleotide level. Genetica. 1997;100:271-9 pubmed..The tetramer composition of retroelement sequences appears to be influenced by host genomic factors like methylase activity. ..
- Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J. The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J. 2001;25:295-303 pubmed..These results suggest that ABI1 and ABI2 act in a negative feedback regulatory loop of the ABA signalling pathway. ..
- Rousset F, Bouchon D, Pintureau B, Juchault P, Solignac M. Wolbachia endosymbionts responsible for various alterations of sexuality in arthropods. Proc Biol Sci. 1992;250:91-8 pubmed..Isopod endosymbionts form a separate lineage, and they can determine feminization as well as cytoplasmic incompatibility. The ability to determine cytoplasmic incompatibility, found in all lineages, is probably ancestral to this group. ..
- Lecain E, Chenivesse X, Spagnoli R, Pompon D. Cloning by metabolic interference in yeast and enzymatic characterization of Arabidopsis thaliana sterol delta 7-reductase. J Biol Chem. 1996;271:10866-73 pubmed..In vitro tests indicated that delta 7-reductase activity is preferentially associated with the endoplasmic reticulum membrane and confirmed the previous finding that NADPH is the reducing agent. ..
- Breda C, Sallaud C, el Turk J, Buffard D, de Kozak I, Esnault R, et al. Defense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles. Mol Plant Microbe Interact. 1996;9:713-9 pubmed..In situ hybridizations indicated that MsPR10-1 was expressed in the vascular bundles adjacent to and distant from the infection site...
- Guigueno A, Dassa J, Belin P, Boquet P. Oversynthesis of a new Escherichia coli small RNA suppresses export toxicity of DsbA'-PhoA unfoldable periplasmic proteins. J Bacteriol. 2001;183:1147-58 pubmed..UptR RNA is the first example of a small RNA implicated in extracytoplasmic toxicity suppression. It appears to offer a new way of suppressing toxicity, and its possible modes of action are discussed. ..
- Marsolier Kergoat M. Asymmetry indices for analysis and prediction of replication origins in eukaryotic genomes. PLoS ONE. 2012;7:e45050 pubmed publisher..However, I show here that the skew jumps at C. albicans centromeres are not related to replication and that replication-associated GC and TA skews in C. albicans have in fact the opposite directions of what was proposed. ..
- Serre M, Garel J. Role of the C-terminal region in the allosteric properties of Escherichia coli phosphofructokinase-1. Eur J Biochem. 1990;189:487-92 pubmed..The carboxy-terminal segment also appears to contribute to the stability of the interactions between subunits: the mutant protein is less stable than the wild type towards denaturation by heat or guanidinium hydrochloride. ..
- Julien J, Samama P, Mallet J. Rat brain glutamic acid decarboxylase sequence deduced from a cloned cDNA. J Neurochem. 1990;54:703-5 pubmed..However, the deduced amino acid sequence of the carboxy-terminal end of the rat protein, downstream of residue 557, is totally different from the cat, whereas it agrees with a published partial peptidic sequence of the rat protein. ..
- Pillet L, Tremeau O, Ducancel F, Drevet P, Zinn Justin S, Pinkasfeld S, et al. Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis. J Biol Chem. 1993;268:909-16 pubmed..These residues appear to be important elements of the curaremimetic function of Ea. Thus, a picture of the contribution of conserved residues to the function of a curaremimetic toxin is proposed on the basis of experimental evidence. ..
- Racki W, Becam A, Nasr F, Herbert C. Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. EMBO J. 2000;19:4524-32 pubmed
- Figueroa N, Wills N, Bossi L. Common sequence determinants of the response of a prokaryotic promoter to DNA bending and supercoiling. EMBO J. 1991;10:941-9 pubmed..Three additional promoter mutations correcting (or alleviating) the effects of the upstream alteration (including a change at -8) are described and discussed. ..
- Motorin Y, Bec G, Tewari R, Grosjean H. Transfer RNA recognition by the Escherichia coli delta2-isopentenyl-pyrophosphate:tRNA delta2-isopentenyl transferase: dependence on the anticodon arm structure. RNA. 1997;3:721-33 pubmed..Our data suggest a recognition mode that implies the interaction of IPTT with the strictly conserved A36A37A38 sequence and the other functional groups located in the minor groove of the anticodon stem. ..
- Pasqualato S, Senic Matuglia F, Renault L, Goud B, Salamero J, Cherfils J. The structural GDP/GTP cycle of Rab11 reveals a novel interface involved in the dynamics of recycling endosomes. J Biol Chem. 2004;279:11480-8 pubmed..Altogether, our study suggests that this mutation impairs a specific subset of Rab11 interactions, possibly those involved in cytoskeleton-based movements driving the slow recycling pathway. ..
- Mirande M, Waller J. Molecular cloning and primary structure of cDNA encoding the catalytic domain of rat liver aspartyl-tRNA synthetase. J Biol Chem. 1989;264:842-7 pubmed..The primary structure of the DRS1 gene product is discussed in relation to the occurrence of two distinct forms of that enzyme. ..
- Berrard S, Brice A, Mallet J. Molecular genetic approach to the study of mammalian choline acetyltransferase. Brain Res Bull. 1989;22:147-53 pubmed..In this article, recent studies concerning the isolation of a cDNA encoding the complete sequence of the porcine enzyme are reported and the potential applications of this probe are discussed. ..
- Moumni M, Kottler M, Counis R. Nucleotide sequence analysis of mRNAs predicts that rat pituitary and gonadal gonadotropin-releasing hormone receptor proteins have identical primary structure. Biochem Biophys Res Commun. 1994;200:1359-66 pubmed..Although post-translational modifications of the GnRH-receptor may differ in the various tissues examined, these data at least clarify the nature and identity of gonadal GnRH receptors. ..
- Firsov D, Elalouf J. Molecular cloning of two rat GRK6 splice variants. Am J Physiol. 1997;273:C953-61 pubmed..The identification of GRK6b extends the family of GRKs. Further studies will be required to establish the tissue and subcellular distribution of this protein and to delineate its physiological role...
- Reisdorf P, Lawrence D, Sivan V, Klising E, Martin M. Alteration of transforming growth factor-beta1 response involves down-regulation of Smad3 signaling in myofibroblasts from skin fibrosis. Am J Pathol. 2001;159:263-72 pubmed..We propose that uncoupling of antiproliferative and profibrotic actions of TGF-beta1 in fibrosis may occur through differential regulation of the activities of Smad2 and Smad3 transcription factors. ..
- Figueroa Bossi N, Coissac E, Netter P, Bossi L. Unsuspected prophage-like elements in Salmonella typhimurium. Mol Microbiol. 1997;25:161-73 pubmed..They are distinct from previously described Fels prophages. Unlike Fels, they are not specific to S. typhimurium strain LT2 since they are both also found in a virulent S. typhimurium isolate (ATCC 14028s)...
- Soler N, Fourmy D, Yoshizawa S. Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB. J Mol Biol. 2007;370:728-41 pubmed publisher..The structures show that tandem WH motifs not only provide an excellent scaffold for RNA binding but can also have an active role in the function of protein-RNA complexes...
- Haeussler M, Joly J. When needles look like hay: how to find tissue-specific enhancers in model organism genomes. Dev Biol. 2011;350:239-54 pubmed publisher..The review is accompanied by a list of general guidelines for such a project. ..
- Porrúa O, Libri D. A bacterial-like mechanism for transcription termination by the Sen1p helicase in budding yeast. Nat Struct Mol Biol. 2013;20:884-91 pubmed publisher..We also show that termination is inhibited by RNA-DNA hybrids. Our results elucidate the role of Sen1p in controlling pervasive transcription. ..
- Kratassiouk G, Pritchard L, Cuvellier S, Vislovukh A, Meng Q, Groisman R, et al. The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M. Cell Cycle. 2016;15:667-77 pubmed publisher..Our data show that translational regulation by RISC and CPEB1 is essential in cell cycle control and, most importantly, is coordinated, and can be switched from inhibition to activation during the cell cycle. ..
- Gillet D, Ducancel F, Pradel E, Leonetti M, Menez A, Boulain J. Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities. Protein Eng. 1992;5:273-8 pubmed..We propose to use the system described in this paper for fast preparation of properly folded disulfide-containing enzymatic probes. ..
- Minet M, Dufour M, Lacroute F. Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. Gene. 1992;121:393-6 pubmed..In contrast, it has only 21% identity with the S. cerevisiae enzyme, which probably reflects the cytosolic location of the enzyme in the latter organism. ..
- Swanson R, Conesa C, Lefebvre O, Carles C, Ruet A, Quemeneur E, et al. Isolation of TFC1, a gene encoding one of two DNA-binding subunits of yeast transcription factor tau (TFIIIC). Proc Natl Acad Sci U S A. 1991;88:4887-91 pubmed..The TFC1-encoded protein contains a potential helix-turn-helix structure and an acidic carboxyl-terminal domain, a feature characteristic of some DNA-binding proteins and transcriptional regulators. ..
- Powell J, Hsu Y, Weyler W, Chen S, Salach J, Andrikopoulos K, et al. The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes. Biochem J. 1989;259:407-13 pubmed..Analysis of mRNAs coding for MAO enzymes showed a heterogeneity of transcripts consistent with several different forms of monoamine oxidase. ..
- Laufs J, Traut W, Heyraud F, Matzeit V, Rogers S, Schell J, et al. In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proc Natl Acad Sci U S A. 1995;92:3879-83 pubmed..In addition, we show that the Rep protein has a joining activity, suggesting that it acts as a terminase, thus resolving the nascent viral single strand into genome-sized units...
- Smid A, Riva M, Bouet F, Sentenac A, Carles C. The association of three subunits with yeast RNA polymerase is stabilized by A14. J Biol Chem. 1995;270:13534-40 pubmed..These results demonstrate that A14 is a genuine subunit of RNA polymerase I and suggest that A14 plays a role in the stability of a subgroup of subunits. ..
- Dujardin G, Kermorgant M, Slonimski P, Boucherie H. Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae. Gene. 1994;139:127-32 pubmed..cerevisiae GMPS. Finally, using microsequencing, we have identified one of the polypeptides, which is overproduced in response to GUA1 amplification, as corresponding to GUA1. ..
- Hermann Le Denmat S, Werner M, Sentenac A, Thuriaux P. Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing. Mol Cell Biol. 1994;14:2905-13 pubmed..This accounts for the isolation of FHL1 as a dosage-dependent suppressor and suggests that rRNA processing depends on a still-unidentified RNA polymerase III transcript. ..
- Esnault Y, Blondel M, Deshaies R, Scheckman R, Kepes F. The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum. EMBO J. 1993;12:4083-93 pubmed..Consistent with its essential role in protein translocation, the Sss1 protein localizes to the ER and homologues were detected in higher eukaryotes. ..
- Nait Kaoudjt R, Williams R, Guiard B, Gervais M. Some DNA targets of the yeast CYP1 transcriptional activator are functionally asymmetric--evidence of two half-sites with different affinities. Eur J Biochem. 1997;244:301-9 pubmed..d) CYP1-(1-200)-peptide is usually a monomer in solution but binds DNA as a dimer. Finally, we found evidence for the presence of two half-sites with different measured affinities in the asymmetric sequences of some CYP1 targets. ..
- Francin M, Kaminska M, Kerjan P, Mirande M. The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain. J Biol Chem. 2002;277:1762-9 pubmed publisher..Our results provide new insights into tRNA(Lys) channeling in eukaryotic cells and shed new light on the possible requirement of native LysRS for triggering tRNA(3)Lys packaging into human immunodeficiency virus, type 1 viral particles...
- Hamel P, Saint Georges Y, de Pinto B, Lachacinski N, Altamura N, Dujardin G. Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana. Mol Microbiol. 2004;51:307-17 pubmed..Finally, we demonstrate that Pic2p is a mitochondrial protein and that its steady state level increases at high temperature. We propose that Pic2p is a minor form of mPic which plays a role under specific stress conditions. ..
- Expert Bezançon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer J, et al. hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 2004;279:38249-59 pubmed..These data indicate that the G-rich sequence is a composite element that acts as an enhancer or as a silencer, depending on which proteins bind to them. ..
- Yoshizawa S, Rasubala L, Ose T, Kohda D, Fourmy D, Maenaka K. Structural basis for mRNA recognition by elongation factor SelB. Nat Struct Mol Biol. 2005;12:198-203 pubmed publisher..Notably, RNA binding does not induce a major conformational change in the WH motif. The structure reveals a new mode of RNA recognition with a geometry that allows the complex to wrap around the small ribosomal subunit...
- Francin M, Mirande M. Identity elements for specific aminoacylation of a tRNA by mammalian lysyl-tRNA synthetase bearing a nonspecific tRNA-interacting factor. Biochemistry. 2006;45:10153-60 pubmed..The possibility that interaction of the full-length cognate tRNA with the synthetase is required to induce the catalytic center of the enzyme into a productive conformation is discussed. ..
- Richier B, Michard Vanhée C, Lamouroux A, Papin C, Rouyer F. The clockwork orange Drosophila protein functions as both an activator and a repressor of clock gene expression. J Biol Rhythms. 2008;23:103-16 pubmed publisher..In addition, the absence of CWO induces alterations of PER and CLK phosphorylation cycles. Our results indicate that, in vivo, CWO modulates clock gene expression through both repressor and activator transcriptional functions. ..
- Oulion S, Debiais Thibaud M, d Aubenton Carafa Y, Thermes C, Da Silva C, Bernard Samain S, et al. Evolution of Hox gene clusters in gnathostomes: insights from a survey of a shark (Scyliorhinus canicula) transcriptome. Mol Biol Evol. 2010;27:2829-38 pubmed publisher..This survey of the transcriptome of the Hox gene clusters of a shark shows that the high complexity observed in mammals is a gnathostome ancestral feature. ..
- van Dijk E, Jaszczyszyn Y, Thermes C. Library preparation methods for next-generation sequencing: tone down the bias. Exp Cell Res. 2014;322:12-20 pubmed publisher..For each type of bias we discuss methods for improvement with a view to providing some useful advice to the researcher who wishes to convert any kind of raw nucleic acid into an NGS library. ..
- Thomas D, Jacquemin I, Surdin Kerjan Y. MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:1719-27 pubmed..This regulation was shown to depend on both MET4 and CBF1. The obtained results suggest that the binding of CBF1 to its cognate sequences increases the ability of MET4 to stimulate transcription of the MET genes. ..
- Minvielle Sebastia L, Winsor B, Bonneaud N, Lacroute F. Mutations in the yeast RNA14 and RNA15 genes result in an abnormal mRNA decay rate; sequence analysis reveals an RNA-binding domain in the RNA15 protein. Mol Cell Biol. 1991;11:3075-87 pubmed