Experts and Doctors on drosophila melanogaster in Toulouse, Languedoc Roussillon Midi Pyrénées, France


Locale: Toulouse, Languedoc Roussillon Midi Pyrénées, France
Topic: drosophila melanogaster

Top Publications

  1. Germain M, Blanchet S, Loyau A, Danchin Ã. Mate-choice copying in Drosophila melanogaster: Impact of demonstration conditions and male-male competition. Behav Processes. 2016;125:76-84 pubmed publisher
    ..Moreover, we did not detect any significant effect of male-male competition on female choice. This study thus provides further evidence for MCC in D. melanogaster and expands on the necessary methodology for detailed studies. ..
  2. Le Bourg Ã. Life-time protection against severe heat stress by exposing young Drosophila melanogaster flies to a mild cold stress. Biogerontology. 2016;17:409-15 pubmed publisher
    ..g. 25 to 22 °C would increase their lifespan and they could survive a strong stress at an age when flies kept at 25 °C are dead. ..
  3. Ruysschaert T, Marque A, Duteyrat J, Lesieur S, Winterhalter M, Fournier D. Liposome retention in size exclusion chromatography. BMC Biotechnol. 2005;5:11 pubmed
    ..Retention of liposomes by size exclusion gels is a dynamic and reversible process, which should be accounted for to control lipid loss and sample contamination during chromatography. ..
  4. Estrada Mondaca S, Fournier D. Stabilization of recombinant Drosophila acetylcholinesterase. Protein Expr Purif. 1998;12:166-72 pubmed
    ..Another way to reduce instability is the addition of a reversible inhibitor or polyethylene glycol 3350. ..
  5. Fresquet N, Fournier D, Gauthier M. A new attempt to assess the effect of learning processes on the cholinergic system: studies on fruitflies and honeybees. Comp Biochem Physiol B Biochem Mol Biol. 1998;119:349-53 pubmed
    ..This experiment did not reveal any modulatory effect of the learning acquisition level on the AChE activity in insects as was previously reported in honeybees. ..
  6. Plaza S, Prince F, Adachi Y, Punzo C, Cribbs D, Gehring W. Cross-regulatory protein-protein interactions between Hox and Pax transcription factors. Proc Natl Acad Sci U S A. 2008;105:13439-44 pubmed publisher
    ..These results demonstrate that the homeodomain supports multiple molecular regulatory functions in addition to protein-DNA and protein-RNA interactions; it is also involved in protein-protein interactions. ..
  7. Siadat O, Lougarre A, Lamouroux L, Ladurantie C, Fournier D. The effect of engineered disulfide bonds on the stability of Drosophila melanogaster acetylcholinesterase. BMC Biochem. 2006;7:12 pubmed
    ..The quantities produced by this mutant were the same as in wild-type flies. Addition of a disulfide bridge may either stabilize or unstabilize proteins. One bond out of the 7 tested provided significant stabilisation. ..
  8. Verollet C, Colombié N, Daubon T, Bourbon H, Wright M, Raynaud Messina B. Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation. J Cell Biol. 2006;172:517-28 pubmed
    ..In conclusion, gamma-TuRCs are required for assembly of fully functional spindles, but we suggest that gamma-TuSC could be targeted to the centrosomes, which is where basic microtubule assembly activities are maintained. ..
  9. Roussigne M, Kossida S, Lavigne A, Clouaire T, Ecochard V, Glories A, et al. The THAP domain: a novel protein motif with similarity to the DNA-binding domain of P element transposase. Trends Biochem Sci. 2003;28:66-9 pubmed
    ..Our results suggest that the THAP domain is a novel example of a DBD that is shared between cellular proteins and transposases from mobile genomic parasites. ..

More Information


  1. Fernandes I, Chanut Delalande H, Ferrer P, Latapie Y, Waltzer L, Affolter M, et al. Zona pellucida domain proteins remodel the apical compartment for localized cell shape changes. Dev Cell. 2010;18:64-76 pubmed publisher
    ..These results on ZPD proteins therefore reveal a functional subcompartmentalization of the apical membrane and its role in the polarized control of epithelial cell shape during development. ..
  2. Boublik Y, Saint Aguet P, Lougarre A, Arnaud M, Villatte F, Estrada Mondaca S, et al. Acetylcholinesterase engineering for detection of insecticide residues. Protein Eng. 2002;15:43-50 pubmed
  3. Le Bourg E -. Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Lett. 2001;498:183-6 pubmed
    ..It is concluded that antioxidant enzymes are probably poorly connected to the normal aging process, but they allow the organism to cope with stressful conditions. ..
  4. Gobert V, Osman D, Bras S, Augé B, Boube M, Bourbon H, et al. A genome-wide RNA interference screen identifies a differential role of the mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila. Mol Cell Biol. 2010;30:2837-48 pubmed publisher
    ..More generally, we suggest that the set of conserved factors identified herein may regulate GATA/RUNX activity in mammals. ..
  5. Estrada Mondaca S, Lougarre A, Fournier D. Drosophila acetylcholinesterase: effect of post-translational [correction of post-traductional] modifications on the production in the baculovirus system and substrate metabolization. Arch Insect Biochem Physiol. 1998;38:84-90 pubmed
  6. Villatte F, Marcel V, Estrada Mondaca S, Fournier D. Engineering sensitive acetylcholinesterase for detection of organophosphate and carbamate insecticides. Biosens Bioelectron. 1998;13:157-64 pubmed
    ..D. melanogaster is 8-fold more sensitive than E. electricus enzyme and this sensitivity has been increased to 12-fold by introducing a mutation at position 408. ..
  7. Noselli S, Vincent A. The Drosophila melanogaster ribosomal protein L17A-encoding gene. Gene. 1992;118:273-8 pubmed
    ..The 5' end of the D. melanogaster rpL17A mRNA contains a polypyrimidine tract displayed by several mammalian rp genes and involved in translational control of their expression. ..
  8. Payre F, Yanicostas C, Vincent A. Serendipity delta, a Drosophila zinc finger protein present in embryonic nuclei at the onset of zygotic gene transcription. Dev Biol. 1989;136:469-80 pubmed
    ..These data are consistent with the sry delta protein being a transcription factor, with a role in zygotic activation of general cellular functions. ..
  9. Tempesta C, Hijazi A, Moussian B, Roch F. Boudin trafficking reveals the dynamic internalisation of specific septate junction components in Drosophila. PLoS ONE. 2017;12:e0185897 pubmed publisher
    ..Our data reveal that the SJ contain a set of components exhibiting a high membrane turnover, a feature that could contribute in a tissue-specific manner to the morphogenetic plasticity of these adhesion structures...
  10. Schott S, Ambrosini A, Barbaste A, Benassayag C, Gracia M, Proag A, et al. A fluorescent toolkit for spatiotemporal tracking of apoptotic cells in living Drosophila tissues. Development. 2017;144:3840-3846 pubmed publisher
    ..They overcome the limitations of other apoptosis detection methods developed so far and, notably, they can be combined with any kind of fluorophore. ..
  11. Zanet J, Stramer B, Millard T, Martin P, Payre F, Plaza S. Fascin is required for blood cell migration during Drosophila embryogenesis. Development. 2009;136:2557-65 pubmed publisher
    ..Together, these results offer a first glimpse into the mechanisms regulating Fascin function during normal development, which might be relevant for understanding the impact of Fascin in cancers. ..
  12. Bataillé L, Augé B, Ferjoux G, Haenlin M, Waltzer L. Resolving embryonic blood cell fate choice in Drosophila: interplay of GCM and RUNX factors. Development. 2005;132:4635-44 pubmed
    ..These results provide new insight into the mechanisms controlling Drosophila hematopoiesis and establish the basis for an original model for the resolution of the choice of blood cell fate. ..
  13. Ferrer P, Crozatier M, Salles C, Vincent A. Interspecific comparison of Drosophila serendipity delta and beta: multimodular structure of these C2H2 zinc finger proteins. J Mol Evol. 1994;38:263-73 pubmed
    ..pseudoobscura sry delta gene rescues the sry delta mutant phenotype. Convergence of genetic and structural data on the sry proteins supports a multimodular function and mode of evolution of these C2H2 finger proteins. ..
  14. Raynaud Messina B, Mazzolini L, Moisand A, Cirinesi A, Wright M. Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cells. J Cell Sci. 2004;117:5497-507 pubmed
    ..They point to an alternative mechanism of centrosomal microtubule assembly that contributes to the formation of abnormal, albeit partially functional, mitotic spindles. ..
  15. Waltzer L, Ferjoux G, Bataillé L, Haenlin M. Cooperation between the GATA and RUNX factors Serpent and Lozenge during Drosophila hematopoiesis. EMBO J. 2003;22:6516-25 pubmed
    ..We propose that interaction and cooperation between GATA and RUNX factors may play an important role in regulating blood cell formation from Drosophila to mammals. ..
  16. Le Bourg E. The NF-kB like factor DIF has weaker effects on Drosophila melanogaster immune defenses than previously thought. J Comp Physiol B. 2011;181:741-50 pubmed publisher
    ..However, DIF flies had a similar survival time after infection as control ones or a slightly lower one, which shows that the effect of DIF is weaker than previously thought. ..
  17. Mojica Vázquez L, Benetah M, Baanannou A, Bernat Fabre S, Deplancke B, Cribbs D, et al. Tissue-specific enhancer repression through molecular integration of cell signaling inputs. PLoS Genet. 2017;13:e1006718 pubmed publisher
    ..Our findings shed light on how the concerted action of two transcriptional repressors, in response to cell signaling inputs, shapes and refines gene expression along the limb proximo-distal axis in a timely manner. ..
  18. Lefrère V, Vincent A, Amalric F. Drosophila melanogaster poly(A)-binding protein: cDNA cloning reveals an unusually long 3'-untranslated region of the mRNA, also present in other eukaryotic species. Gene. 1993;126:295-6 pubmed
  19. Makki R, Meister M, Pennetier D, Ubeda J, Braun A, Daburon V, et al. A short receptor downregulates JAK/STAT signalling to control the Drosophila cellular immune response. PLoS Biol. 2010;8:e1000441 pubmed publisher
  20. Liang J, Lacroix L, Gamot A, Cuddapah S, Queille S, Lhoumaud P, et al. Chromatin immunoprecipitation indirect peaks highlight long-range interactions of insulator proteins and Pol II pausing. Mol Cell. 2014;53:672-81 pubmed publisher
    ..Our data illustrate how indirect peaks may decipher gene regulatory networks through specific long-range interactions. ..
  21. Bras S, Martin Lannerée S, Gobert V, Augé B, Breig O, Sanial M, et al. Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis. Proc Natl Acad Sci U S A. 2012;109:4986-91 pubmed publisher
    ..Thus, we propose that the regulation of RUNX protein levels is a conserved feature of MLF family members that could be critical for normal and pathological blood cell development. ..
  22. Foussard H, Ferrer P, Valenti P, Polesello C, Carreno S, Payre F. LRCH proteins: a novel family of cytoskeletal regulators. PLoS ONE. 2010;5:e12257 pubmed publisher
    ..Here we report the characterization of a novel family of evolutionary conserved proteins, which display specific features of cytoskeletal scaffolding proteins, referred to as LRCHs...
  23. Lefrère V, Vincent A, Amalric F. Drosophila melanogaster poly(A)-binding protein: cDNA cloning reveals an unusually long 3'-untranslated region of the mRNA, also present in other eukaryotic species [corrected]. Gene. 1990;96:219-25 pubmed
    ..melanogaster PABP mRNA is shown to exist also in other eukaryotes including vertebrate species. It suggests that important regulatory sequences intrinsic to the PABP mRNA are present within this 3'-untranslated region. ..
  24. Osman D, Gobert V, Ponthan F, Heidenreich O, Haenlin M, Waltzer L. A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO. Proc Natl Acad Sci U S A. 2009;106:12043-8 pubmed publisher
    ..Therefore Drosophila provides a promising genetically tractable model to investigate the conserved basis of leukemogenesis and to open avenues in AML therapy. ..
  25. Chelouah S, Monod Wissler C, Bailly C, Barret J, Guilbaud N, Vispé S, et al. An integrated Drosophila model system reveals unique properties for F14512, a novel polyamine-containing anticancer drug that targets topoisomerase II. PLoS ONE. 2011;6:e23597 pubmed publisher
  26. Colombié N, Verollet C, Sampaio P, Moisand A, Sunkel C, Bourbon H, et al. The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus. Mol Biol Cell. 2006;17:272-82 pubmed
    ..The nature of a minimal gamma-tubulin complex necessary for proper microtubule organization in the metazoans is discussed. ..
  27. Brand S, Pichoff S, Noselli S, Bourbon H. Novel Drosophila melanogaster genes encoding RRM-type RNA-binding proteins identified by a degenerate PCR strategy. Gene. 1995;154:187-92 pubmed
    ..21 (1993) 3699-3704]. ..
  28. Polesello C, Delon I, Valenti P, Ferrer P, Payre F. Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis. Nat Cell Biol. 2002;4:782-9 pubmed
    ..This study also demonstrates the requirement of Dmoesin for the specific organization of cortical microfilaments in nurse cells and, consequently, mutations in Dmoesin produce severe defects in cell shape. ..
  29. Marcel V, Estrada Mondaca S, Magne F, Stojan J, Klaebe A, Fournier D. Exploration of the Drosophila acetylcholinesterase substrate activation site using a reversible inhibitor (Triton X-100) and mutated enzymes. J Biol Chem. 2000;275:11603-9 pubmed
    ..These results led to the hypothesis that substrate binding at this site affects the entrance of another substrate molecule into the active site cleft. ..
  30. Berrada S, Fournier D. Transposition-mediated transcriptional overexpression as a mechanism of insecticide resistance. Mol Gen Genet. 1997;256:348-54 pubmed
    ..This strain had only one extra Ace gene, which overexpressed acetylcholinesterase. Thus, following transposition, resistance resulted from the overexpression of a single copy of the gene and not from gene amplification. ..
  31. Marcel V, Palacios L, Pertuy C, Masson P, Fournier D. Two invertebrate acetylcholinesterases show activation followed by inhibition with substrate concentration. Biochem J. 1998;329 ( Pt 2):329-34 pubmed
    ..These triphasic kinetics can be explained by the presence of two enzymes with different kinetic behaviours or more probably by the existence of a single enzyme regulated by the substrate concentration. ..
  32. Dewar A, Wystrach A, Philippides A, Graham P. Neural coding in the visual system of Drosophila melanogaster: How do small neural populations support visually guided behaviours?. PLoS Comput Biol. 2017;13:e1005735 pubmed publisher
    ..This leads us to suggest that in order to understand the properties of neural systems, one must consider how perceptual circuits put information at the service of behaviour...
  33. Ferjoux G, Augé B, Boyer K, Haenlin M, Waltzer L. A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells. Dev Biol. 2007;305:726-34 pubmed
    ..Based on our observations, we propose that this GATA/RUNX cis-regulatory module may be reiteratively used during hematopoietic development through evolution. ..
  34. Ibnsouda S, Schweisguth F, de Billy G, Vincent A. Relationship between expression of serendipity alpha and cellularisation of the Drosophila embryo as revealed by interspecific transformation. Development. 1993;119:471-83 pubmed
    ..melanogaster and D. pseudoobscura sry alpha RNAs have different subcellular localisations, suggesting that this specific localization has no conserved role in targeting the sry alpha protein to the apical membranes...
  35. MILLER M, Chen A, Gobert V, Augé B, Beau M, Burlet Schiltz O, et al. Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis. PLoS Genet. 2017;13:e1006932 pubmed publisher
    ..Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo. ..
  36. Boube M, Benassayag C, Seroude L, Cribbs D. Ras1-mediated modulation of Drosophila homeotic function in cell and segment identity. Genetics. 1997;146:619-28 pubmed
    ..Taken together our data support a role for Ras1-mediated cell signaling in the homeotic control of segmental differentiation. ..
  37. Hijazi A, Haenlin M, Waltzer L, Roch F. The Ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila. PLoS ONE. 2011;6:e17763 pubmed publisher
    ..We propose that vertebrate Ly6 proteins could fulfill analogous roles in tight junctions and/or paranodal septate junctions. ..
  38. Le Bourg E. A cold stress applied at various ages can increase resistance to heat and fungal infection in aged Drosophila melanogaster flies. Biogerontology. 2011;12:185-93 pubmed publisher
    ..Therefore, a mild stress can have positive effects on longevity and resistance to strong stresses not only when used at a young age, but also at older ages. ..
  39. Krzemien J, Crozatier M, Vincent A. Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism. Int J Dev Biol. 2010;54:1117-25 pubmed publisher
    ..We end up by integrating studies in Drosophila within a more general picture of insect hematopoiesis and hemocyte homeostasis. ..
  40. Lougarre A, Bride J, Fournier D. Is the insect glutathione S-transferase I gene family intronless?. Insect Mol Biol. 1999;8:141-3 pubmed
    ..But sequences of the untranslated 5' end of transcripts revealed the presence of an intron in housefly and Drosophila genes suggesting that most insect GSTI genes are in fact interrupted. ..
  41. Baanannou A, Mojica Vázquez L, Darras G, Couderc J, Cribbs D, Boube M, et al. Drosophila distal-less and Rotund bind a single enhancer ensuring reliable and robust bric-a-brac2 expression in distinct limb morphogenetic fields. PLoS Genet. 2013;9:e1003581 pubmed publisher
    ..Our findings indicate that accuracy, reliability and robustness of developmental gene expression do not necessarily require cis-regulatory information redundancy. ..
  42. Breig O, Bras S, Martinez Soria N, Osman D, Heidenreich O, Haenlin M, et al. Pontin is a critical regulator for AML1-ETO-induced leukemia. Leukemia. 2014;28:1271-9 pubmed publisher
    ..Concordantly, PONTIN depletion inhibited leukemic self-renewal and caused cell cycle arrest. All together our data suggest that the upregulation of PONTIN by AML1-ETO participate in the oncogenic growth of t(8;21) cells...
  43. Renalier M, Nicoloso M, Qu L, Bachellerie J. SnoRNA U21 is also intron-encoded in Drosophila melanogaster but in a different host-gene as compared to warm-blooded vertebrates. FEBS Lett. 1996;379:212-6 pubmed
    ..melanogaster and vertebrates, in addition to the hallmark box C and box D motifs, corresponds precisely to the 13-nt complementary to rRNA, pointing to an important role of the pairing of U21 to pre-rRNA. ..
  44. Avet Rochex A, Boyer K, Polesello C, Gobert V, Osman D, Roch F, et al. An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. BMC Dev Biol. 2010;10:65 pubmed publisher
  45. Vincent A, Crozatier M. Neither too much nor too little: reactive oxygen species levels regulate Drosophila hematopoiesis. J Mol Cell Biol. 2010;2:74-5 pubmed publisher
    ..In vivo experiments in the Drosophila hematopoietic organ now challenge this view by showing that high ROS levels are intrinsically required to prime myeloid-like progenitors to differentiate. ..
  46. Bouissou A, Verollet C, Sousa A, Sampaio P, Wright M, Sunkel C, et al. {gamma}-Tubulin ring complexes regulate microtubule plus end dynamics. J Cell Biol. 2009;187:327-34 pubmed publisher
    ..We propose that, in addition to its role in nucleation, the gamma-TuRC associated to MTs may regulate their dynamics by limiting catastrophes. ..
  47. Jullien D, Crozatier M, Kas E. cDNA sequence and expression pattern of the Drosophila melanogaster PAPS synthetase gene: a new salivary gland marker. Mech Dev. 1997;68:179-86 pubmed
    ..We discuss the possibility that the pattern of expression of the DmPAPSS gene might reflect a major role for sulfation in mucus biosynthesis at the end of Drosophila embryogenesis. ..
  48. Payre F, Buono P, Vanzo N, Vincent A. Two types of zinc fingers are required for dimerization of the serendipity delta transcriptional activator. Mol Cell Biol. 1997;17:3137-45 pubmed
    ..A systematic deletion analysis shows that interaction between this proposed Cys2/Cys2 motif and a classical Cys2/His2 zinc finger mediates homodimerization, which is required for DNA binding cooperativity. ..
  49. Brand S, Bourbon H. The developmentally-regulated Drosophila gene rox8 encodes an RRM-type RNA binding protein structurally related to human TIA-1-type nucleolysins. Nucleic Acids Res. 1993;21:3699-704 pubmed
    ..Molecular characterization of rox8 represents the first step in a genetic analysis of the potential roles of a TIA-1-related protein in RNA metabolism and/or programmed cell death in Drosophila. ..
  50. Peyrefitte S, Kahn D, Haenlin M. New members of the Drosophila Myc transcription factor subfamily revealed by a genome-wide examination for basic helix-loop-helix genes. Mech Dev. 2001;104:99-104 pubmed
    ..Together with previous reports, our data suggest that, similar to networks involved in neurogenesis and myogenesis, the network of Myc-related genes has been globally conserved throughout evolution. ..