Experts and Doctors on fungal proteins in Bordeaux, Aquitaine Limousin Poitou Charentes, France


Locale: Bordeaux, Aquitaine Limousin Poitou Charentes, France
Topic: fungal proteins

Top Publications

  1. Berthelot K, Lecomte S, Géan J, Immel F, Cullin C. A yeast toxic mutant of HET-s((218-289)) prion displays alternative intermediates of amyloidogenesis. Biophys J. 2010;99:1239-46 pubmed publisher
    ..With the methods used, no intermediates were observed for the nontoxic amyloid; however, under the same experimental conditions, the toxic mutant displayed visible oligomeric and fibrillar intermediates. ..
  2. Legrand P, Pinaud N, Minvielle Sebastia L, Fribourg S. The structure of the CstF-77 homodimer provides insights into CstF assembly. Nucleic Acids Res. 2007;35:4515-22 pubmed
    ..Mapping experiments identify the C-terminal region of Rna14p, the yeast counterpart of CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue. ..
  3. Dupont C, Rigoulet M, Beauvoit B, Guerin B. Mitochondrial modifications in a single nuclear mutant of Saccharomyces cerevisiae affected in cAMP-dependent protein phosphorylation. Curr Genet. 1990;17:507-13 pubmed
    ..It is also possible that the modification in cAMP-dependent protein kinase regulation which appeared to occur in this mutant affects protein(s) involved in mitochondrial biogenesis. ..
  4. Roumanie O, Weinachter C, Larrieu I, Crouzet M, Doignon F. Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae. FEBS Lett. 2001;506:149-56 pubmed
    ..In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac-like GTPase Rho5. ..
  5. Coustou Linares V, Maddelein M, Begueret J, Saupe S. In vivo aggregation of the HET-s prion protein of the fungus Podospora anserina. Mol Microbiol. 2001;42:1325-35 pubmed
    ..Together, these results further support the model that the [Het-s] element corresponds to an abnormal self-perpetuating aggregated form of the HET-s protein. ..
  6. Noel T. The cellular and molecular defense mechanisms of the Candida yeasts against azole antifungal drugs. J Mycol Med. 2012;22:173-8 pubmed publisher
    ..This review proposes to explore the different molecular strategies that are used by Candida yeasts to fight azole antifungals...
  7. Gauthier S, Coulpier F, Jourdren L, Merle M, Beck S, Konrad M, et al. Co-regulation of yeast purine and phosphate pathways in response to adenylic nucleotide variations. Mol Microbiol. 2008;68:1583-94 pubmed publisher
    ..This work reveals that adenylic nucleotide availability is a key signal used by yeast to co-ordinate phosphate utilization and purine synthesis. ..
  8. Balguerie A, Dos Reis S, Coulary Salin B, Chaignepain S, Sabourin M, Schmitter J, et al. The sequences appended to the amyloid core region of the HET-s prion protein determine higher-order aggregate organization in vivo. J Cell Sci. 2004;117:2599-610 pubmed
  9. Pinan Lucarré B, Paoletti M, Dementhon K, Coulary Salin B, Clavé C. Autophagy is induced during cell death by incompatibility and is essential for differentiation in the filamentous fungus Podospora anserina. Mol Microbiol. 2003;47:321-33 pubmed
    ..In addition, we found that pspA and idi-7 null mutations confer differentiation defects such as the absence of female reproductive structures, indicating that autophagy is required for differentiation in Podospora...

More Information


  1. Pinson B, Gabrielsen O, Daignan Fornier B. Redox regulation of AMP synthesis in yeast: a role of the Bas1p and Bas2p transcription factors. Mol Microbiol. 2000;36:1460-9 pubmed
    ..This hypothesis was further substantiated in a two hybrid experiment showing that Bas1p/Bas2p interaction is affected by oxidative stress. ..
  2. Couzin N, Trezeguet V, Le Saux A, Lauquin G. Cloning of the gene encoding the mitochondrial adenine nucleotide carrier of Schizosaccharomyces pombe by functional complementation in Saccharomyces cerevisiae. Gene. 1996;171:113-7 pubmed
    ..Hybridization experiments with ordered libraries of Sp genomic DNA led to the physical mapping (chromosome II, NotI-B region) and the isolation of the Sp ANC1 gene. We also conclude that a single-copy gene encodes the Sp Anc. ..
  3. Bernard P, Hardwick K, Javerzat J. Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. J Cell Biol. 1998;143:1775-87 pubmed
    ..However, unlike its vertebrate counterpart, a pool of Bub1 remains centromere-associated at metaphase and even until telophase. We discuss the possibility of a role for the Bub1 kinase after the metaphase-anaphase transition. ..
  4. Navarro P, Durrens P, Aigle M. Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae. Biochim Biophys Acta. 1997;1343:187-92 pubmed
    ..Moreover, if a tagged Rvs 167p protein was immuno adsorbed under non-denaturing conditions, it brought along the Rvs161p protein. These results confirmed the hypothesis of an in vivo complex between the two proteins. ..
  5. Benkemoun L, Sabate R, Malato L, Dos Reis S, Dalstra H, Saupe S, et al. Methods for the in vivo and in vitro analysis of [Het-s] prion infectivity. Methods. 2006;39:61-7 pubmed
    ..In addition, we report several methods that can be used to infect Podospora with recombinant HET-s amyloid. ..
  6. Chirio M, Brethes D, Napias C, Grandier Vazeille X, Rakotomanana F, Chevallier J. Photoaffinity labelling of the purine-cytosine permease of Saccharomyces cerevisiae. Eur J Biochem. 1990;194:293-9 pubmed
    ..Therefore this polypeptide corresponds to the purine-cytosine permease. ..
  7. Daskalov A, Paoletti M, Ness F, Saupe S. Genomic clustering and homology between HET-S and the NWD2 STAND protein in various fungal genomes. PLoS ONE. 2012;7:e34854 pubmed publisher
    ..Het-s] induces cell death when interacting with HET-S, an allelic variant of HET-s. When templated by [Het-s], HET-S undergoes a trans-conformation, relocates to the cell membrane and induces toxicity...
  8. Pinan Lucarré B, Clavé C. Monitoring autophagy in the filamentous fungus Podospora anserina. Methods Enzymol. 2008;451:251-70 pubmed publisher
    ..During the incompatibility reaction, autophagy might protect cells from cell death as suggested by accelerated cell death observed in autophagy mutants. ..
  9. Donzeau M, Bourdineaud J, Lauquin G. Regulation by low temperatures and anaerobiosis of a yeast gene specifying a putative GPI-anchored plasma membrane protein [corrected]. Mol Microbiol. 1996;20:449-59 pubmed
    ..This protein, detected in the plasma membrane fraction, is shown to be exposed on the cell surface via a glycosyl-phosphatidylinositol membrane anchoring. ..
  10. Sivadon P, Peypouquet M, Doignon F, Aigle M, Crouzet M. Cloning of the multicopy suppressor gene SUR7: evidence for a functional relationship between the yeast actin-binding protein Rvs167 and a putative membranous protein. Yeast. 1997;13:747-61 pubmed
    ..Moreover, the putative transmembranous character of Sur7p suggests a membrane localization of the Rvs function, a localization which is consistent with the different rvs phenotypes and the actin-Rvs167p interaction. ..
  11. Roumanie O, Peypouquet M, Bonneu M, Thoraval D, Doignon F, Crouzet M. Evidence for the genetic interaction between the actin-binding protein Vrp1 and the RhoGAP Rgd1 mediated through Rho3p and Rho4p in Saccharomyces cerevisiae. Mol Microbiol. 2000;36:1403-14 pubmed
    ..This protein structure might act with myosins in various actin cytoskeleton-based activities, in co-operation with a Rho3p/Rho4p signalling pathway that is negatively regulated by Rgd1p GAP activity. ..
  12. Roumanie O, Peypouquet M, Thoraval D, Doignon F, Crouzet M. Functional interactions between the VRP1-LAS17 and RHO3-RHO4 genes involved in actin cytoskeleton organization in Saccharomyces cerevisiae. Curr Genet. 2002;40:317-25 pubmed
    ..In addition, other observations lead us to propose that Rvs167p may act as a linking protein between the two cellular elements. ..
  13. Balguerie A, Sivadon P, Bonneu M, Aigle M. Rvs167p, the budding yeast homolog of amphiphysin, colocalizes with actin patches. J Cell Sci. 1999;112 ( Pt 15):2529-37 pubmed
    ..Finally, the amphiphysin-conserved N-terminal domain of Rvs167p, called the BAR domain, was required but not sufficient for the correct localization of the protein. ..
  14. Mouassite M, Camougrand N, Schwob E, Demaison G, Laclau M, Guerin M. The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation. Yeast. 2000;16:905-19 pubmed
    ..Our data suggest that Sun4p and Uth1p might contribute to the regulated process of cell wall morphogenesis and septation. ..
  15. Lefebvre Legendre L, Vaillier J, Benabdelhak H, Velours J, Slonimski P, di Rago J. Identification of a nuclear gene (FMC1) required for the assembly/stability of yeast mitochondrial F(1)-ATPase in heat stress conditions. J Biol Chem. 2001;276:6789-96 pubmed
    ..However, unlike Atp12p and Atp11p, Fmc1p is not required in normal growth conditions (28--30 degrees C). We propose that Fmc1p is required for the proper folding/stability or functioning of Atp12p in heat stress conditions. ..
  16. Saupe S, Clave C, Sabourin M, Begueret J. Characterization of hch, the Podospora anserina homolog of the het-c heterokaryon incompatibility gene of Neurospora crassa. Curr Genet. 2000;38:39-47 pubmed
    ..This suggested that hch does not function as a het gene. However, heterologous expression of the N. crassa het-cPA allele in P. anserina triggers a growth defect reminiscent of the het-c incompatibility reaction. ..
  17. Espagne E, Balhadère P, Penin M, Barreau C, Turcq B. HET-E and HET-D belong to a new subfamily of WD40 proteins involved in vegetative incompatibility specificity in the fungus Podospora anserina. Genetics. 2002;161:71-81 pubmed
    ..In particular, the amino acids present on the upper face of the predicted beta-propeller structure defined by this domain may confer the incompatible interaction specificity...
  18. Crapeau M, Marchal C, Cullin C, Maillet L. The cellular concentration of the yeast Ure2p prion protein affects its propagation as a prion. Mol Biol Cell. 2009;20:2286-96 pubmed publisher
    ..We propose a model to explain how prion protein overexpression can produce a detrimental effect on prion propagation and why Ure2p(Sp) might be more sensitive to such effects than Ure2p(Sc). ..
  19. Férandon C, Moukha S, Callac P, Benedetto J, Castroviejo M, Barroso G. The Agaricus bisporus cox1 gene: the longest mitochondrial gene and the largest reservoir of mitochondrial group i introns. PLoS ONE. 2010;5:e14048 pubmed publisher
    ..bisporus cox1 gene exhibits a peculiar dynamics of intron keeping and catching, leading to the largest collection of mitochondrial group I introns reported to date in a Eukaryote...