Experts and Doctors on viral proteins in Sweden


Locale: Sweden
Topic: viral proteins

Top Publications

  1. Munir M, Linde A, Zohari S, Ståhl K, Baule C, Holm K, et al. Complete genome analysis of an avian paramyxovirus type 1 strain isolated in 1994 from an asymptomatic black-headed gull (Larus ridibundus) in southern Sweden. Avian Dis. 2010;54:923-30 pubmed
    ..Waterfowl have been considered to act as a reservoir for APMV-1 and, therefore, it is important to broaden the knowledge of viruses circulating within this population. ..
  2. Liu Y, Haggård Ljungquist E. Functional characterization of the P2 A initiator protein and its DNA cleavage site. Virology. 1996;216:158-64 pubmed
  3. Nilsson M, Ljungberg J, Richter J, Kiefer T, Magnusson M, Lieber A, et al. Development of an adenoviral vector system with adenovirus serotype 35 tropism; efficient transient gene transfer into primary malignant hematopoietic cells. J Gene Med. 2004;6:631-41 pubmed
    ..Our study will facilitate the generation of fiber-retargeted adenoviral vectors and enable transient genetic manipulation of primary malignant hematopoietic cells. ..
  4. Wu S, Haag L, Sjöberg M, Garoff H, Hammar L. The dynamic envelope of a fusion class II virus. E3 domain of glycoprotein E2 precursor in Semliki Forest virus provides a unique contact with the fusion protein E1. J Biol Chem. 2008;283:26452-60 pubmed publisher
    ..This finding reevaluates the role of the precursor from being only a provider of a shield over the fusion loop to a structural playmate in formation of the fusogenic architecture. ..
  5. Nordholm J, da Silva D, Damjanovic J, Dou D, Daniels R. Polar residues and their positional context dictate the transmembrane domain interactions of influenza A neuraminidases. J Biol Chem. 2013;288:10652-60 pubmed publisher
  6. Lindeberg J, Ebendal T. Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator. Nucleic Acids Res. 1999;27:1552-4 pubmed
    ..Here we report that these constructs function effectively in COS cells. The data suggest that these cassettes will be appropriate for 3' targeting of mouse genes. ..
  7. Onnheim K, Ekblad M, Görander S, Bergstrom T, Liljeqvist J. Vaccination with the Secreted Glycoprotein G of Herpes Simplex Virus 2 Induces Protective Immunity after Genital Infection. Viruses. 2016;8:110 pubmed publisher
    ..We show that a sgG-2 based vaccine is highly effective and can be considered as a novel candidate in the development of a prophylactic vaccine against HSV-2 infection. ..
  8. Sandegren L, Sjoberg B. Self-splicing of the bacteriophage T4 group I introns requires efficient translation of the pre-mRNA in vivo and correlates with the growth state of the infected bacterium. J Bacteriol. 2007;189:980-90 pubmed
    ..This is the first time that environmental effects on bacterial growth have been linked to the regulation of splicing of phage introns. ..
  9. Bergström Lind S, Artemenko K, Elfineh L, Zhao Y, Bergquist J, Pettersson U. Post translational modifications in adenovirus type 2. Virology. 2013;447:104-11 pubmed publisher
    ..Nitrated tyrosines were also identified in pII. Lysine acetylations were detected in pII and pVI. The findings make the Ad2 virion much more complex than hitherto believed. ..

More Information


  1. Henriksson Peltola P, Sehlén W, Haggård Ljungquist E. Determination of the DNA-binding kinetics of three related but heteroimmune bacteriophage repressors using EMSA and SPR analysis. Nucleic Acids Res. 2007;35:3181-91 pubmed
    ..Thus, the kinetics of complex formation and the nature of the complexes formed differ extensively between these very closely related phages. ..
  2. Nasir W, Frank M, Koppisetty C, Larson G, Nyholm P. Lewis histo-blood group ?1,3/?1,4 fucose residues may both mediate binding to GII.4 noroviruses. Glycobiology. 2012;22:1163-72 pubmed publisher
    ..4 strains. Our results are relevant for understanding the evolution of norovirus binding specificities and for in silico design of future antiviral therapeutics. ..
  3. Hidmark A, Nordström E, Dosenovic P, Forsell M, Liljestrom P, Karlsson Hedestam G. Humoral responses against coimmunized protein antigen but not against alphavirus-encoded antigens require alpha/beta interferon signaling. J Virol. 2006;80:7100-10 pubmed
  4. Okroj M, Mark L, Stokowska A, Wong S, Rose N, Blackbourn D, et al. Characterization of the complement inhibitory function of rhesus rhadinovirus complement control protein (RCP). J Biol Chem. 2009;284:505-14 pubmed publisher
    ..This makes RRV a superior model for future in vivo investigations of complement evasion, as RCP does not play a supportive role in viral attachment as KCP does. ..
  5. Blomström A, Belak S, Fossum C, McKillen J, Allan G, Wallgren P, et al. Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Res. 2009;146:125-9 pubmed publisher
    ..By comparative analysis of the nucleotide sequences and phylogenetic studies we propose that this is a novel porcine parvovirus, with genetic relationship to bocaviruses. ..
  6. Massad T, Skaar K, Nilsson H, Damberg P, Henriksson Peltola P, Haggård Ljungquist E, et al. Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats. Nucleic Acids Res. 2010;38:7778-90 pubmed publisher
    ..The structure provides insight into the mechanisms behind the mutants of P2 C causing dimer disruption, temperature sensitivity and insensitivity to the P4 antirepressor. ..
  7. Lood R, Collin M. Characterization and genome sequencing of two Propionibacterium acnes phages displaying pseudolysogeny. BMC Genomics. 2011;12:198 pubmed publisher
    ..acnes phages sequenced, but not to genomes of other phages isolated from Propionibacteria. The phage genomes are not integrated in the bacterial genome, but instead, most likely have a pseudolysogenic life cycle. ..
  8. Muylaert I, Tang K, Elias P. Replication and recombination of herpes simplex virus DNA. J Biol Chem. 2011;286:15619-24 pubmed publisher
  9. Schlegel S, Löfblom J, Lee C, Hjelm A, Klepsch M, Strous M, et al. Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3). J Mol Biol. 2012;423:648-59 pubmed publisher
  10. Montelius I, Liljas L, Unge T. Sequential removal of Ca2+ from satellite tobacco necrosis virus. Crystal structure of two EDTA-treated forms. J Mol Biol. 1990;212:331-43 pubmed
    ..One possible RNA-binding site was found in the maps of both crystal types, and the same site could also be localized in the high-resolution map of native STNV. ..
  11. Sundström K, Stoltz M, Lagerqvist N, Lundkvist A, Nemirov K, Klingstrom J. Characterization of two substrains of Puumala virus that show phenotypes that are different from each other and from the original strain. J Virol. 2011;85:1747-56 pubmed publisher
    ..Importantly, this implies that the reported differences in phenotypes for hantaviruses might depend more on chance due to spontaneous mutations during passage than inherited true differences between hantaviruses. ..
  12. Zetterberg H, Jansson A, Rymo L, Chen F, Karlsson A, Klein G, et al. The Epstein-Barr virus ZEBRA protein activates transcription from the early lytic F promoter by binding to a promoter-proximal AP-1-like site. J Gen Virol. 2002;83:2007-14 pubmed
  13. Kis L, Takahara M, Nagy N, Klein G, Klein E. IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines. Blood. 2006;107:2928-35 pubmed
  14. Renberg Eriksson S, Ahlgren Berg A, DeGrooth J, Haggård Ljungquist E. Characterization of the developmental switch region of bacteriophage P2 Hy dis. Virology. 2001;290:199-210 pubmed
    ..The P4 E protein was shown to derepress the developmental switch of P2 Hy dis in a plasmid-based derepression assay...
  15. Olsson M, Tang K, Persson C, Wilhelmsson L, Billeter M, Elias P. Stepwise evolution of the herpes simplex virus origin binding protein and origin of replication. J Biol Chem. 2009;284:16246-55 pubmed publisher
    ..Our results reveal a stepwise evolutionary history of the herpes simplex virus replicon and suggest that replicon divergence contributed to the formation of major branches of the herpesvirus family...
  16. Odegrip R, Schoen S, Haggård Ljungquist E, Park K, Chattoraj D. The interaction of bacteriophage P2 B protein with Escherichia coli DnaB helicase. J Virol. 2000;74:4057-63 pubmed
  17. Timmusk S, Merlot E, Lövgren T, Järvekülg L, Berg M, Fossum C. Regulator of G protein signalling 16 is a target for a porcine circovirus type 2 protein. J Gen Virol. 2009;90:2425-36 pubmed publisher
    ..Furthermore, poRGS16 appeared to participate in the translocation of the ORF3 protein into the cell nucleus, suggesting that the observed interaction may play an important role in the infection biology of porcine circovirus. ..
  18. Li H, Wang J, Wilhelmsson H, Hansson A, Thoren P, Duffy J, et al. Genetic modification of survival in tissue-specific knockout mice with mitochondrial cardiomyopathy. Proc Natl Acad Sci U S A. 2000;97:3467-72 pubmed
  19. Nenonen N, Hannoun C, Larsson C, Bergstrom T. Marked genomic diversity of norovirus genogroup I strains in a waterborne outbreak. Appl Environ Microbiol. 2012;78:1846-52 pubmed publisher
    ..Recognition of NoV diversity in a cluster of patients provided a useful warning marker of waterborne contamination in the Lilla Edet outbreak. ..
  20. Veijola J, Pettersson R. Transient association of calnexin and calreticulin with newly synthesized G1 and G2 glycoproteins of uukuniemi virus (family Bunyaviridae). J Virol. 1999;73:6123-7 pubmed
    ..Persson and R. F. Pettersson, J. Cell Biol. 112:257-266, 1991). Taking all of this into consideration, we conclude that the folding of G1 and G2 in the ER is catalyzed by at least four different folding factors...
  21. Mark L, Spiller O, Okroj M, Chanas S, Aitken J, Wong S, et al. Molecular characterization of the rhesus rhadinovirus (RRV) ORF4 gene and the RRV complement control protein it encodes. J Virol. 2007;81:4166-76 pubmed
    ..These data provide the foundation for studies into the biological significance of gammaherpesvirus complement regulatory proteins in a tractable, non-human primate model...
  22. Savenkov E, Valkonen J. Silencing of a viral RNA silencing suppressor in transgenic plants. J Gen Virol. 2002;83:2325-35 pubmed
    ..Methylation of the HC(pro) transgene followed recovery. These data show that the transgene mRNA for a silencing suppressor can be silenced by a presumably 'strong' silencing inducer (replicating homologous virus). ..
  23. Zhao Z, Tang K, Muylaert I, Samuelsson T, Elias P. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem. 2017;292:15489-15500 pubmed publisher
    ..Our results suggest that efficient expression of replication-dependent ?2 late genes is, at least in part, regulated by CDK9 dependent co- and/or post-transcriptional events involving SPT5 and ICP27. ..
  24. Liu T, Renberg S, Haggård Ljungquist E. Derepression of prophage P2 by satellite phage P4: cloning of the P4 epsilon gene and identification of its product. J Virol. 1997;71:4502-8 pubmed
    ..The ash9 mutation, which is located upstream of the epsilon gene, enhances the E-mediated derepression of the Pe promoter. The purified E protein shows no specific DNA binding activity, and the implications of this are discussed...
  25. Frumerie C, Sylwan L, Ahlgren Berg A, Haggård Ljungquist E. Cooperative interactions between bacteriophage P2 integrase and its accessory factors IHF and Cox. Virology. 2005;332:284-94 pubmed
    ..The Int and Cox proteins also bind cooperatively to attP...
  26. Krzywkowski T, Nilsson M. Fidelity of RNA templated end-joining by chlorella virus DNA ligase and a novel iLock assay with improved direct RNA detection accuracy. Nucleic Acids Res. 2017;45:e161 pubmed publisher
    ..We show significant increase in PBCV-1 ligation fidelity in the iLock probe assay configuration for RNA detection...
  27. Eriksson J, Haggård Ljungquist E. The multifunctional bacteriophage P2 cox protein requires oligomerization for biological activity. J Bacteriol. 2000;182:6714-23 pubmed
    ..Furthermore we show that oligomerization is necessary for the biological activity by characterizing different cox mutants and that oligomerization is mediated by the C-terminal region...
  28. Liu T, Hagg rd Ljungquist E. The transcriptional switch of bacteriophage WPhi, a P2-related but heteroimmune coliphage. J Virol. 1999;73:9816-26 pubmed
    ..However, in contrast to P2 Cox, WPhi Cox is unable to activate the P4 Pll promoter...
  29. Bergström Lind S, Artemenko K, Elfineh L, Zhao Y, Bergquist J, Pettersson U. The phosphoproteome of the adenovirus type 2 virion. Virology. 2012;433:253-61 pubmed publisher
    ..Structural modeling showed that one site in the hexon is located on the outer side of the virus and could be of importance for the virus when attaching and entering cells...
  30. Muradrasoli S, Mohamed N, Hornyák A, Fohlman J, Olsen B, Belak S, et al. Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks (Anas platyrhynchos). J Virol Methods. 2009;159:277-87 pubmed publisher
    ..Ducks may spread and harbour CoVs. NQPCR can detect a wide range of CoVs, as illustrated for humans and ducks...
  31. Wagner S, Klepsch M, Schlegel S, Appel A, DRAHEIM R, Tarry M, et al. Tuning Escherichia coli for membrane protein overexpression. Proc Natl Acad Sci U S A. 2008;105:14371-6 pubmed publisher
    ..The generality and simplicity of our approach make it ideal for high-throughput applications. ..
  32. Forsman A, Ruetschi U, Ekholm J, Rymo L. Identification of intracellular proteins associated with the EBV-encoded nuclear antigen 5 using an efficient TAP procedure and FT-ICR mass spectrometry. J Proteome Res. 2008;7:2309-19 pubmed publisher
    ..Our study also confirms previously identified interactors including HA95, Hsp70, Hsc70, Hsp27, HAX-1, Prolyl 4-hydroxylase, S3a, and alpha- and beta-tubulin. ..
  33. Overby A, Popov V, Pettersson R, Neve E. The cytoplasmic tails of Uukuniemi Virus (Bunyaviridae) G(N) and G(C) glycoproteins are important for intracellular targeting and the budding of virus-like particles. J Virol. 2007;81:11381-91 pubmed
    ..Together, these results demonstrate that the cytoplasmic tails of both G(N) and G(C) contain specific information necessary for efficient virus particle generation...
  34. Hagg rd Ljungquist E, Jacobsen E, Rishovd S, Six E, Nilssen O, Sunshine M, et al. Bacteriophage P2: genes involved in baseplate assembly. Virology. 1995;213:109-21 pubmed
    ..P4 mutations that partially compensate for this defect of gene V lie in the P4 capsid size determination gene, sid...
  35. Ahlgren Berg A, Henriksson Peltola P, Sehlén W, Haggård Ljungquist E. A comparison of the DNA binding and bending capacities and the oligomeric states of the immunity repressors of heteroimmune coliphages P2 and WPhi. Nucleic Acids Res. 2007;35:3167-80 pubmed
    ..However, the protein-DNA complexes formed differ, as determined by electrophoretic mobility shift experiments. A difference in spontaneous phage production is observed in isogenic lysogens...
  36. Akerstr m S, Tan Y, Mirazimi A. Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies. FEBS Lett. 2006;580:3799-803 pubmed publisher
    ..Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody...
  37. Karlsson J, Cardoso Palacios C, Nilsson A, Haggård Ljungquist E. Evolution of immunity and host chromosome integration site of P2-like coliphages. J Bacteriol. 2006;188:3923-35 pubmed
    ..The evolution of immunity and integration sites is complex, since it involves interactions both between the phages themselves and between phages and hosts, and often, both regulatory proteins and target DNA must change...
  38. Cartier A, Broberg E, Komai T, Henriksson M, Masucci M. The herpes simplex virus-1 Us3 protein kinase blocks CD8T cell lysis by preventing the cleavage of Bid by granzyme B. Cell Death Differ. 2003;10:1320-8 pubmed
    ..Our data illustrate a new strategy of viral escape, where modification of a cellular proapoptotic substrate may prevent lysis of the infected cells without affecting other T-cell functions. ..
  39. Saha S, Hagg rd Ljungquist E, Nordstr m K. The cox protein of bacteriophage P2 inhibits the formation of the repressor protein and autoregulates the early operon. EMBO J. 1987;6:3191-9 pubmed
  40. Rasul A, Nagy N, Sohlberg E, dori M, Claesson H, Klein G, et al. Simultaneous detection of the two main proliferation driving EBV encoded proteins, EBNA-2 and LMP-1 in single B cells. J Immunol Methods. 2012;385:60-70 pubmed publisher
    ..Consequently, Type IIa lines could not be established from Hodgkin's lymphoma in vitro. We propose that these experimental cultures can be exploited for study of the Type IIa cells...
  41. Winquist J, Abdurakhmanov E, Baraznenok V, Henderson I, Vrang L, Danielson U. Resolution of the interaction mechanisms and characteristics of non-nucleoside inhibitors of hepatitis C virus polymerase. Antiviral Res. 2013;97:356-68 pubmed publisher
    ..These insights have important consequences for the selection and optimization of new allosteric NS5B inhibitors. ..
  42. Lukhovitskaya N, Solovieva A, Boddeti S, Thaduri S, Solovyev A, Savenkov E. An RNA virus-encoded zinc-finger protein acts as a plant transcription factor and induces a regulator of cell size and proliferation in two tobacco species. Plant Cell. 2013;25:960-73 pubmed publisher
    ..It is likely that other members of the conserved C-4 type ZF family of viral proteins instigate reprogramming of plant development by mimicking eukaryotic transcriptional activators...
  43. Muylaert I, Zhao Z, Andersson T, Elias P. Identification of conserved amino acids in the herpes simplex virus type 1 UL8 protein required for DNA synthesis and UL52 primase interaction in the virus replisome. J Biol Chem. 2012;287:33142-52 pubmed
    ..The replication-defective mutations affected conserved amino acids, and similar phenotypes were observed when the corresponding mutations were introduced into EHV-1 UL8. ..
  44. Nilsson M, Belfrage A, Lindström S, Wähling H, Lindquist C, Ayesa S, et al. Synthesis and SAR of potent inhibitors of the Hepatitis C virus NS3/4A protease: exploration of P2 quinazoline substituents. Bioorg Med Chem Lett. 2010;20:4004-11 pubmed publisher
    ..Pharmacokinetic properties in vivo were assessed in rat on selected compounds, where excellent exposure and liver-to-plasma ratios were demonstrated for a member of the 14-membered quinazoline substituted P2 proline urea series...
  45. Kis L, Salamon D, Persson E, Nagy N, Scheeren F, Spits H, et al. IL-21 imposes a type II EBV gene expression on type III and type I B cells by the repression of C- and activation of LMP-1-promoter. Proc Natl Acad Sci U S A. 2010;107:872-7 pubmed publisher
    ..In light of the fact that IL-21 is already in clinical trials for the treatment of multiple malignancies, the in vivo modulation of EBV gene expression by IL-21 might have therapeutic benefits for the EBV-carrying malignancies...
  46. Lindeberg J, Usoskin D, Bengtsson H, Gustafsson A, Kylberg A, Söderström S, et al. Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus. Genesis. 2004;40:67-73 pubmed
    ..This knockin mouse can also be used for tracing cell lineages expressing TH during development. ..
  47. Olsson J, Dasgupta S, Berg O, Nordström K. Eclipse period without sequestration in Escherichia coli. Mol Microbiol. 2002;44:1429-40 pubmed
    ..The causal relationship between the length of the eclipse period and the synchrony of initiations from oriC is discussed. ..
  48. Kekarainen T, Savilahti H, Valkonen J. Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation. Genome Res. 2002;12:584-94 pubmed
    ..The methodology described is applicable to a detailed functional analysis of any viral nucleic acid cloned as DNA and can be used to address many different processes during viral infection cycles. ..
  49. Liu T, Renberg S, Haggård Ljungquist E. The E protein of satellite phage P4 acts as an anti-repressor by binding to the C protein of helper phage P2. Mol Microbiol. 1998;30:1041-50 pubmed
  50. Liu Y, Haggård Ljungquist E. Studies of bacteriophage P2 DNA replication: localization of the cleavage site of the A protein. Nucleic Acids Res. 1994;22:5204-10 pubmed
  51. Mohamed N, Nilsson E, Johansson P, Klingstrom J, Evander M, Ahlm C, et al. Development and evaluation of a broad reacting SYBR-green based quantitative real-time PCR for the detection of different hantaviruses. J Clin Virol. 2013;56:280-5 pubmed publisher
    ..This novel assay provides a powerful tool for diagnosis of hantaviruses from different clades and regions and may also be useful in surveys with the purpose of finding new hantaviruses in rodent or insectivore species. ..
  52. Nilsson A, Haggård Ljungquist E. Evolution of P2-like phages and their impact on bacterial evolution. Res Microbiol. 2007;158:311-7 pubmed
    ..Taken together, this suggests that P2-like phages and their hosts are coevolving...
  53. Kreuze J, Savenkov E, Valkonen J. Complete genome sequence and analyses of the subgenomic RNAs of sweet potato chlorotic stunt virus reveal several new features for the genus Crinivirus. J Virol. 2002;76:9260-70 pubmed
    ..The 5' ends of seven sgRNAs were cloned and sequenced by an approach that provided compelling evidence that the sgRNAs are capped in infected plants, a novel finding for members of the Closteroviridae...
  54. Trier N, Holm B, Slot O, Locht H, Lindegaard H, Svendsen A, et al. Application of synthetic peptides for detection of anti-citrullinated peptide antibodies. Peptides. 2016;76:87-95 pubmed publisher
    ..Our data suggest that citrullinated EBNA-1 peptides may be considered a substrate for the detection of ACPAs and that the presence of Epstein-Barr virus may play a role in the induction of these autoantibodies. ..