Genomes and Genes
Experts and Doctors on ubiquitin protein ligases in United States
Locale: United States
Topic: ubiquitin protein ligases
Publications126 found, 100 shown here
- Loring G, Christensen K, Gerber S, Brenner C. Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination. Cell Cycle. 2008;7:96-105 pubmed..Thus, combined genetic and biochemical analyses indicate that Chf proteins have dual E2 specificity accounting for different functions in the cell cycle. ..
- Wu Q, Kim K, Sampson E, Chen D, Awad H, O Brien T, et al. Induction of an osteoarthritis-like phenotype and degradation of phosphorylated Smad3 by Smurf2 in transgenic mice. Arthritis Rheum. 2008;58:3132-44 pubmed publisher..Overall, these results suggest a role of Smurf2 in the pathogenesis of OA. ..
- Corcoran C, Montalbano J, Sun H, He Q, Huang Y, Sheikh M. Identification and characterization of two novel isoforms of Pirh2 ubiquitin ligase that negatively regulate p53 independent of RING finger domains. J Biol Chem. 2009;284:21955-70 pubmed publisher..It is therefore possible that Pirh2A and the novel Pirh2 isoforms identified in this study may also modulate p53 function by engaging MDM2. ..
- Joshi N, Barot A, Jamison C, Börner G. Pch2 links chromosome axis remodeling at future crossover sites and crossover distribution during yeast meiosis. PLoS Genet. 2009;5:e1000557 pubmed publisher..Pch2 is proposed to reorganize chromosome axes into a tiling array of long-range crossover control modules, resulting in chiasma formation at minimum levels and with maximum spacing...
- Schartner J, Simonson W, Wernimont S, Nettenstrom L, Huttenlocher A, Seroogy C. Gene related to anergy in lymphocytes (GRAIL) expression in CD4+ T cells impairs actin cytoskeletal organization during T cell/antigen-presenting cell interactions. J Biol Chem. 2009;284:34674-81 pubmed publisher..These data demonstrate a novel role for GRAIL in modulating T/APC interactions and provide further insight into the cell biology of anergic T cells. ..
- Hwang C, Shemorry A, Varshavsky A. N-terminal acetylation of cellular proteins creates specific degradation signals. Science. 2010;327:973-7 pubmed publisher..Several examined proteins of diverse functions contained these N-terminal degrons, termed AcN-degrons, which are a prevalent class of degradation signals in cellular proteins. ..
- Han J, Li Q, McCullough L, Kettelkamp C, Formosa T, Zhang Z. Ubiquitylation of FACT by the cullin-E3 ligase Rtt101 connects FACT to DNA replication. Genes Dev. 2010;24:1485-90 pubmed publisher..Origin function is compromised in cells lacking Rtt101 or with an Spt16 mutation. These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication. ..
- Massiah M, Simmons B, Short K, Cox T. Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING. J Mol Biol. 2006;358:532-45 pubmed..One of these is likely to provide the binding interface for Alpha 4 that is required for the localized turnover of the catalytic subunit of PP2A, the major Ser/Thr phosphatase. ..
- Simunovic F, Yi M, Wang Y, Macey L, Brown L, Krichevsky A, et al. Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology. Brain. 2009;132:1795-809 pubmed publisher..Our data provide a 'molecular fingerprint identity' of late-stage Parkinson's disease DA neurons that will advance our understanding of the molecular pathology of this disease. ..
- Lawson T, Sweep M, Schlax P, Bohnsack R, Haas A. Kinetic analysis of the conjugation of ubiquitin to picornavirus 3C proteases catalyzed by the mammalian ubiquitin-protein ligase E3alpha. J Biol Chem. 2001;276:39629-37 pubmed
- Green M, Monser K, Clem R. Ubiquitin protein ligase activity of the anti-apoptotic baculovirus protein Op-IAP3. Virus Res. 2004;105:89-96 pubmed..Thus, we conclude that Op-IAP3 is a functional E3 ubiquitin ligase, and the ability to ubiquitinate pro-apoptotic cellular proteins such as HID may play an important role in the anti-apoptotic function of Op-IAP3. ..
- Angers S, Li T, Yi X, MacCoss M, Moon R, Zheng N. Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. Nature. 2006;443:590-3 pubmed..Together, our structural and proteomic results reveal the structural mechanisms and molecular logic underlying the assembly and versatility of a new family of cullin-RING E3 complexes. ..
- Morales J, Perdew G. Carboxyl terminus of hsc70-interacting protein (CHIP) can remodel mature aryl hydrocarbon receptor (AhR) complexes and mediate ubiquitination of both the AhR and the 90 kDa heat-shock protein (hsp90) in vitro. Biochemistry. 2007;46:610-21 pubmed
- Bhandari D, Trejo J, Benovic J, Marchese A. Arrestin-2 interacts with the ubiquitin-protein isopeptide ligase atrophin-interacting protein 4 and mediates endosomal sorting of the chemokine receptor CXCR4. J Biol Chem. 2007;282:36971-9 pubmed..Taken together, our data suggest that the AIP4.arrestin-2 complex functions on endosomes to regulate sorting of CXCR4 into the degradative pathway. ..
- Varshavsky A. The N-end rule: functions, mysteries, uses. Proc Natl Acad Sci U S A. 1996;93:12142-9 pubmed..In eukaryotes, the N-end rule pathway is a part of the ubiquitin system. I discuss the mechanisms and functions of this pathway, and consider its applications. ..
- Moore R, Boyd L. Analysis of RING finger genes required for embryogenesis in C. elegans. Genesis. 2004;38:1-12 pubmed..5 (an ortholog of rbx1). Embryos from RNAi of the APC11 and rbx1 orthologs were arrested in the cell cycle, confirming the role of these particular RING finger proteins in regulation of the cell cycle. genesis 38:1-12, 2004. ..
- Jiang H, Jiang Q, Feng J. Parkin increases dopamine uptake by enhancing the cell surface expression of dopamine transporter. J Biol Chem. 2004;279:54380-6 pubmed..This function of parkin would enhance the precision of dopaminergic transmission, increase the efficiency of dopamine utilization, and reduce dopamine toxicity on neighboring cells. ..
- Ren Y, Jiang H, Yang F, Nakaso K, Feng J. Parkin protects dopaminergic neurons against microtubule-depolymerizing toxins by attenuating microtubule-associated protein kinase activation. J Biol Chem. 2009;284:4009-17 pubmed publisher..Thus, the results suggest that parkin protects midbrain dopaminergic neurons against microtubule-depolymerizing PD toxins such as rotenone by stabilizing microtubules to attenuate MAP kinase activation. ..
- Jiang H, Jiang Q, Liu W, Feng J. Parkin suppresses the expression of monoamine oxidases. J Biol Chem. 2006;281:8591-9 pubmed..This function may limit the production of reactive oxygen species generated by MAO in dopamine oxidation and would, thus, be beneficial to the survival of dopaminergic neurons. ..
- Tao M, Scacheri P, Marinis J, Harhaj E, Matesic L, Abbott D. ITCH K63-ubiquitinates the NOD2 binding protein, RIP2, to influence inflammatory signaling pathways. Curr Biol. 2009;19:1255-63 pubmed publisher..These findings suggest that ITCH helps regulate NOD2-dependent signal transduction pathways and, as such, may be involved in the pathogenesis of NOD2-mediated inflammatory disease. ..
- Barajas D, Li Z, Nagy P. The Nedd4-type Rsp5p ubiquitin ligase inhibits tombusvirus replication by regulating degradation of the p92 replication protein and decreasing the activity of the tombusvirus replicase. J Virol. 2009;83:11751-64 pubmed publisher..Overall, our data suggest that direct binding between Rsp5p and p92(pol) reduces the stability of p92(pol), with consequent inhibition of TBSV replicase activity. ..
- Varshavsky A. The N-end rule pathway of protein degradation. Genes Cells. 1997;2:13-28 pubmed..Ubiquitin is a 76-residue protein whose covalent conjugation to other proteins plays a role in many biological processes, including cell growth and differentiation. I discuss the current understanding of the N-end rule pathway. ..
- Verma R, Annan R, Huddleston M, Carr S, Reynard G, Deshaies R. Phosphorylation of Sic1p by G1 Cdk required for its degradation and entry into S phase. Science. 1997;278:455-60 pubmed..These data suggest a molecular model of how phosphorylation and proteolysis cooperate to bring about the G1/S transition in budding yeast. ..
- Khoshnan A, Ko J, Watkin E, Paige L, Reinhart P, Patterson P. Activation of the IkappaB kinase complex and nuclear factor-kappaB contributes to mutant huntingtin neurotoxicity. J Neurosci. 2004;24:7999-8008 pubmed..Therefore, aberrant NF-kappaB activation may contribute to the neurodegeneration induced by mutant Htt. ..
- MacKenzie D, Schartner J, Lin J, Timmel A, Jennens Clough M, Fathman C, et al. GRAIL is up-regulated in CD4+ CD25+ T regulatory cells and is sufficient for conversion of T cells to a regulatory phenotype. J Biol Chem. 2007;282:9696-702 pubmed..These data demonstrate that GRAIL is differentially expressed in naturally occurring and peripherally induced CD25(+) T regulatory cells and that the expression of GRAIL is linked to their functional regulatory activity. ..
- Wu Y, Bolduc F, Bell K, Tully T, Fang Y, Sehgal A, et al. A Drosophila model for Angelman syndrome. Proc Natl Acad Sci U S A. 2008;105:12399-404 pubmed publisher..We conclude that dube3a mutants are a valid model for Angelman syndrome, with great potential for identifying the elusive UBE3A substrates relevant to the disease. ..
- Ding X, Richter T, Chen M, Fujii H, Seo Y, Xie M, et al. A rice kinase-protein interaction map. Plant Physiol. 2009;149:1478-92 pubmed publisher..The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed. ..
- Kim H, Huibregtse J. Polyubiquitination by HECT E3s and the determinants of chain type specificity. Mol Cell Biol. 2009;29:3307-18 pubmed publisher..Our results are also consistent with a simple sequential-addition mechanism for polyubiquitination by Rsp5, rather than a mechanism involving the formation of either E2- or E3-linked polyubiquitin chain transfers. ..
- Marchese A. Ubiquitination of chemokine receptors. Methods Enzymol. 2009;460:413-22 pubmed publisher..Here we describe a simple biochemical assay that we have used to study the ubiquitination of CXCR4 that can be easily applied to study the ubiquitination of any GPCR. ..
- Ghislain M, Dohmen R, Levy F, Varshavsky A. Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae. EMBO J. 1996;15:4884-99 pubmed..The discovery of the Ufd3p--Cdc48p complex and the finding that this complex is a part of the Ub system open up a new direction for studies of the function of Ub in the cell cycle and membrane dynamics. ..
- Mosser E, Kasanov J, Forsberg E, Kay B, Ney P, Bresnick E. Physical and functional interactions between the transactivation domain of the hematopoietic transcription factor NF-E2 and WW domains. Biochemistry. 1998;37:13686-95 pubmed..Mutation of PPXY-1, but not PPXY-2, inhibited the transactivation function of NF-E2, providing support for the hypothesis that WW domain interactions are important for NF-E2-mediated transactivation. ..
- Thomas C, Chouinard M, Cox M, Parsons S, Stallings Mann M, Garcia R, et al. Spontaneous activation and signaling by overexpressed epidermal growth factor receptors in glioblastoma cells. Int J Cancer. 2003;104:19-27 pubmed..The results also suggest that in some GBMs, signals from overexpressed EGFRs contribute to the constitutive phosphorylation of Erk, but these signals may not required for the constitutive activation of PI3K or AKT1. ..
- Pedmale U, Liscum E. Regulation of phototropic signaling in Arabidopsis via phosphorylation state changes in the phototropin 1-interacting protein NPH3. J Biol Chem. 2007;282:19992-20001 pubmed
- Sanduja S, Kaza V, Dixon D. The mRNA decay factor tristetraprolin (TTP) induces senescence in human papillomavirus-transformed cervical cancer cells by targeting E6-AP ubiquitin ligase. Aging (Albany NY). 2009;1:803-17 pubmed..These findings demonstrate the ability of TTP to act as a tumor suppressor by inhibiting the E6-AP pathway and indicate TTP loss to be a critical event during HPV-mediated carcinogenesis. ..
- Tabb A, Utsugi T, Wooten Kee C, Sasaki T, Edling S, Gump W, et al. Genes encoding ribosomal proteins Rps0A/B of Saccharomyces cerevisiae interact with TOM1 mutants defective in ribosome synthesis. Genetics. 2001;157:1107-16 pubmed..Ribosome synthesis is therefore included among the cellular processes governed by members of the hect-domain-containing E3 ubiquitin-protein ligase family. ..
- Choi P, Golts N, Snyder H, Chong M, Petrucelli L, Hardy J, et al. Co-association of parkin and alpha-synuclein. Neuroreport. 2001;12:2839-43 pubmed..These results suggest that parkin interacts with alpha-synuclein and could contribute to the pathophysiology of PD more generally than was previously considered. ..
- Gray W, Hellmann H, Dharmasiri S, Estelle M. Role of the Arabidopsis RING-H2 protein RBX1 in RUB modification and SCF function. Plant Cell. 2002;14:2137-44 pubmed..Hypermodification of CUL1 disrupts SCF(TIR1) function, suggesting that cycles of RUB conjugation and removal are important for SCF activity. ..
- Horn E, Albor A, Liu Y, El Hizawi S, Vanderbeek G, Babcock M, et al. RING protein Trim32 associated with skin carcinogenesis has anti-apoptotic and E3-ubiquitin ligase properties. Carcinogenesis. 2004;25:157-67 pubmed..We propose a model in which Trim32 activities as an E3-ubiquitin ligase favor initiated cell survival in carcinogenesis by blocking UVB-induced TNFalpha apoptotic signaling. ..
- Cohen D, Zhou M. Molecular genetics of familial renal cell carcinoma syndromes. Clin Lab Med. 2005;25:259-77 pubmed..Understanding of the molecular pathways of these genes will have a significant impact on the diagnosis and treatment of familial and sporadic RCC. ..
- Dupont Versteegden E, Nagarajan R, Beggs M, Bearden E, Simpson P, Peterson C. Identification of cold-shock protein RBM3 as a possible regulator of skeletal muscle size through expression profiling. Am J Physiol Regul Integr Comp Physiol. 2008;295:R1263-73 pubmed publisher..We conclude that a cold-shock response may be part of a compensatory mechanism in muscles undergoing atrophy to preserve remaining muscle mass and that RBM3 may be a therapeutic target to prevent muscle loss. ..
- Shai A, Pitot H, Lambert P. E6-associated protein is required for human papillomavirus type 16 E6 to cause cervical cancer in mice. Cancer Res. 2010;70:5064-73 pubmed publisher..Nevertheless, in cervical carcinogenesis studies, there was a complete loss of the oncogenic potential of E6 in mice nulligenic for E6AP. Thus, E6AP is absolutely required for E6 to cause cervical cancer. ..
- Chavez J, Lamanna J. Activation of hypoxia-inducible factor-1 in the rat cerebral cortex after transient global ischemia: potential role of insulin-like growth factor-1. J Neurosci. 2002;22:8922-31 pubmed..Our study suggest that activation of HIF-1 might be part of the mechanism by which IGF-1 promotes cell survival after cerebral ischemia. ..
- Petrucelli L, Dickson D, Kehoe K, Taylor J, Snyder H, Grover A, et al. CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation. Hum Mol Genet. 2004;13:703-14 pubmed..Hsp70/CHIP may therefore play an important role in the pathogenesis of tauopathies and also represents a potential therapeutic target. ..
- McClung J, Kavazis A, Whidden M, Deruisseau K, Falk D, Criswell D, et al. Antioxidant administration attenuates mechanical ventilation-induced rat diaphragm muscle atrophy independent of protein kinase B (PKB Akt) signalling. J Physiol. 2007;585:203-15 pubmed..Further, these results also indicate that differential regulation of diaphragmatic IGF-1-PI3K-Akt signalling exists during the early and late stages of MV. ..
- Nayak R, Farris K, Pintel D. E4Orf6-E1B-55k-dependent degradation of de novo-generated adeno-associated virus type 5 Rep52 and capsid proteins employs a cullin 5-containing E3 ligase complex. J Virol. 2008;82:3803-8 pubmed publisher..Degradation of AAV5 proteins can be inhibited by a dominant-negative ubiquitin that prevents chain elongation or by small interfering RNA directed against cullin 5. ..
- Xu Z, Kohli E, Devlin K, Bold M, Nix J, Misra S. Interactions between the quality control ubiquitin ligase CHIP and ubiquitin conjugating enzymes. BMC Struct Biol. 2008;8:26 pubmed publisher..CHIP's interactions with UbcH5, Ube2e2 and Ubc13-Uev1a are consistent with the notion that Ubc13-Uev1a may work sequentially with other E2 enzymes to carry out K63-linked polyubiquitination of CHIP substrates. ..
- Lockhart P, O Farrell C, Farrer M. It's a double knock-out! The quaking mouse is a spontaneous deletion of parkin and parkin co-regulated gene (PACRG). Mov Disord. 2004;19:101-4 pubmed..Homozygous Quaking mice show a complete loss of PRKN and PACRG mRNA and protein. These mice will constitute a useful additional model for studies of the molecular role of parkin and PACRG in neurodegeneration. ..
- Sarkar M, Magliery T. Re-engineering a split-GFP reassembly screen to examine RING-domain interactions between BARD1 and BRCA1 mutants observed in cancer patients. Mol Biosyst. 2008;4:599-605 pubmed publisher..The split frGFP system may also be generally useful for other proteins and cell types, as the split-Venus system has proven to be in mammalian cells. ..
- Lee J, Brauweiler A, Rudolph M, Hooper J, Drabkin H, Gemmill R. The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways. Mol Cancer Res. 2010;8:93-106 pubmed publisher..Moreover, high-molecular weight ubiquitylated proteins were observed in eIF3 immunoprecipitations from TRC8-overexpressing cells. Thus, TRC8 function may provide a regulatory link between the lipid and protein biosynthetic pathways. ..
- Wang H, Mo P, Ren S, Yan C. Activating transcription factor 3 activates p53 by preventing E6-associated protein from binding to E6. J Biol Chem. 2010;285:13201-10 pubmed publisher..These results indicate that ATF3 plays a key role in a mechanism defending against HPV-induced carcinogenesis, and could serve as a novel therapeutic target for HPV-positive cancers. ..
- Mohni K, Livingston C, Cortez D, Weller S. ATR and ATRIP are recruited to herpes simplex virus type 1 replication compartments even though ATR signaling is disabled. J Virol. 2010;84:12152-64 pubmed publisher..Thus, ATR and ATRIP may function outside the context of the canonical ATR damage signaling pathway during HSV-1 infection to participate in the viral life cycle. ..
- Hong Y, Rogers R, Matunis M, Mayhew C, Goodson M, Park Sarge O, et al. Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modification. J Biol Chem. 2001;276:40263-7 pubmed..Mutation of lysine 298 also results in a significant decrease in stress-induced transcriptional activity of HSF1 in vivo. This work implicates SUMO-1 modification as an important modulator of HSF1 function in response to stress. ..
- Lossie A, Whitney M, Amidon D, Dong H, Chen P, Theriaque D, et al. Distinct phenotypes distinguish the molecular classes of Angelman syndrome. J Med Genet. 2001;38:834-45 pubmed..Clinically, we were unable to distinguish between UPD and ID patients, suggesting that 15q11-q13 contains the only significant maternally expressed imprinted genes on chromosome 15. ..
- Xie Y, Varshavsky A. UFD4 lacking the proteasome-binding region catalyses ubiquitination but is impaired in proteolysis. Nat Cell Biol. 2002;4:1003-7 pubmed..This multiple-site binding may function to slow down dissociation of the substrate from the proteasome and to facilitate the unfolding of substrate through ATP-dependent movements of the chaperone subunits of the 19S particle. ..
- Corcoran C, Huang Y, Sheikh M. The p53 paddy wagon: COP1, Pirh2 and MDM2 are found resisting apoptosis and growth arrest. Cancer Biol Ther. 2004;3:721-5 pubmed..Future studies in this field will now face the challenge of elucidating the physiological significance of three molecules all apparently able to independently facilitate p53 degradation and abrogate its function. ..
- Johnstone K, DuBose A, Futtner C, Elmore M, Brannan C, Resnick J. A human imprinting centre demonstrates conserved acquisition but diverged maintenance of imprinting in a mouse model for Angelman syndrome imprinting defects. Hum Mol Genet. 2006;15:393-404 pubmed..This maternal imprinting defect results in expression of maternal Ube3a-as and repression of Ube3a in cis, providing evidence that Ube3a is regulated by its antisense and creating the first reported mouse model for AS imprinting defects...
- Liu M, Rakowski B, Gershburg E, Weisend C, Lucas O, Schmidt E, et al. ICP0 antagonizes ICP4-dependent silencing of the herpes simplex virus ICP0 gene. PLoS ONE. 2010;5:e8837 pubmed publisher..Therefore, our findings appear to add to the growing list of inexplicable similarities that point to a common evolutionary ancestry between the herpesviruses and tailed bacteriophage...
- Meza J, Brzovic P, King M, Klevit R. Mapping the functional domains of BRCA1. Interaction of the ring finger domains of BRCA1 and BARD1. J Biol Chem. 1999;274:5659-65 pubmed
- Wohlfert E, Callahan M, Clark R. Resistance to CD4+CD25+ regulatory T cells and TGF-beta in Cbl-b-/- mice. J Immunol. 2004;173:1059-65 pubmed
- Nodzon L, Xu W, Wang Y, Pi L, Chakrabarty P, Song W. The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis. Plant J. 2004;40:996-1006 pubmed..Treatment with auxin increases the expression of XBAT32 in the primary root and partially rescues the lateral root defect in xbat32-1 mutant plants. Thus, XBAT32 is a novel ubiquitin ligase required for lateral root initiation. ..
- Gardner R, Nelson Z, Gottschling D. Degradation-mediated protein quality control in the nucleus. Cell. 2005;120:803-15 pubmed..We propose that San1p-mediated degradation acts as the last line of proteolytic defense against the deleterious accumulation of aberrant proteins in the nucleus and that analogous systems exist in other eukaryotes. ..
- Wilkinson D, Weller S. Herpes simplex virus type I disrupts the ATR-dependent DNA-damage response during lytic infection. J Cell Sci. 2006;119:2695-703 pubmed..The HSV-1 immediate early protein ICP0 is sufficient to induce the redistribution of ATRIP. This is the first report that a virus can disrupt the usually tight colocalization of ATR and ATRIP. ..
- Huang R, Kowalski D, Minderman H, Gandhi N, Johnson E. Small ubiquitin-related modifier pathway is a major determinant of doxorubicin cytotoxicity in Saccharomyces cerevisiae. Cancer Res. 2007;67:765-72 pubmed..Cumulatively, these results reveal that SUMO modification of proteins mediates the doxorubicin cytotoxicity in yeast, at least partially, by modification of septins and of proteins that control the intracellular drug concentration. ..
- Lawson Yuen A, Liu D, Han L, Jiang Z, Tsai G, Basu A, et al. Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice. Brain Res. 2007;1180:1-6 pubmed..The male mice experience early death. Analysis of Ube3a mRNA and protein levels in the Mecp2(R168X) male mice showed no significant difference in expression compared to their wild type littermates. ..
- Schmidt R, Park C, Ahmed A, Gundelach J, Reed N, Cheng S, et al. Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue. Cancer Res. 2007;67:11798-810 pubmed..Thus, SIAH may offer a novel therapeutic target to halt tumor growth and ameliorate RAS-mediated pancreatic cancer. ..
- Wei S, Yang H, Chuang H, Yang J, Kulp S, Lu P, et al. A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells. J Biol Chem. 2008;283:26759-70 pubmed publisher..Moreover, we obtained evidence that this beta-TrCP-dependent degradation takes part in controlling cyclin D1 turnover when cancer cells undergo glucose starvation, which endows physiological relevance to this novel mechanism. ..
- Zhu Q, Wani G, Arab H, El Mahdy M, Ray A, Wani A. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites. DNA Repair (Amst). 2009;8:262-73 pubmed publisher..These data demonstrate that successful NER of genomic lesions and prompt CAF-1-mediated chromatin restoration link uH2A incorporation at the sites of damage repair within chromatin. ..
- Choi Y, Butterworth M, Malladi S, Duckett C, Cohen G, Bratton S. The E3 ubiquitin ligase cIAP1 binds and ubiquitinates caspase-3 and -7 via unique mechanisms at distinct steps in their processing. J Biol Chem. 2009;284:12772-82 pubmed publisher..Thus, cIAPs likely suppress apoptosis, at least in part, by facilitating the ubiquitination and turnover of active effector caspases in cells. ..
- Roth J, Singleton S, Feng J, Garrick M, Paradkar P. Parkin regulates metal transport via proteasomal degradation of the 1B isoforms of divalent metal transporter 1. J Neurochem. 2010;113:454-64 pubmed publisher..These results demonstrate that parkin is the E3 ligase responsible for ubiquitination of the 1B species of DMT1. ..
- Hsouna A, Nallamothu G, Kose N, Guinea M, Dammai V, Hsu T. Drosophila von Hippel-Lindau tumor suppressor gene function in epithelial tubule morphogenesis. Mol Cell Biol. 2010;30:3779-94 pubmed publisher..Importantly, the regulatory function in tubular morphogenesis is conserved in the mammalian system, as conditional knockout of Vhl in mouse kidney also resulted in similar cell motility and lumen phenotypes. ..
- Jeong R, Chandra Shekara A, Barman S, Navarre D, Klessig D, Kachroo A, et al. Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated viral defense by negatively regulating an E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010;107:13538-43 pubmed publisher..We propose that CRY2/PHOT2 negatively regulate the proteasome-mediated degradation of HRT, likely via COP1, and blue-light relieves this repression resulting in HRT degradation...
- Meacham G, Patterson C, Zhang W, Younger J, Cyr D. The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation. Nat Cell Biol. 2001;3:100-5 pubmed..CHIP is a co-chaperone that converts Hsc70 from a protein-folding machine into a degradation factor that functions in endoplasmic-reticulum quality control. ..
- Conaway R, Conaway J. The von Hippel-Lindau tumor suppressor complex and regulation of hypoxia-inducible transcription. Adv Cancer Res. 2002;85:1-12 pubmed
- Shen R, Chen M, Wang Y, Kaneki H, Xing L, O Keefe R, et al. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation. J Biol Chem. 2006;281:3569-76 pubmed..Smurf1-induced Runx2 degradation serves as a negative regulatory mechanism for the BMP-Smad-Runx2 signaling pathway. ..
- Dai M, Shi D, Jin Y, Sun X, Zhang Y, Grossman S, et al. Regulation of the MDM2-p53 pathway by ribosomal protein L11 involves a post-ubiquitination mechanism. J Biol Chem. 2006;281:24304-13 pubmed..These results suggest that L11, unlike L5 and L23, differentially regulates the levels of ubiquitinated p53 and MDM2 and inhibits the turnover and activity of MDM2 through a post-ubiquitination mechanism. ..
- Haugarvoll K, Toft M, Ross O, Stone J, Heckman M, White L, et al. ELAVL4, PARK10, and the Celts. Mov Disord. 2007;22:585-7 pubmed..Our data suggest that the association between ELAVL4 and PD previously observed might be explained by a Celtic-founder effect. ..
- Tan X, Calderon Villalobos L, Sharon M, Zheng C, Robinson C, Estelle M, et al. Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature. 2007;446:640-5 pubmed..By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor. ..
- Mahrour N, Redwine W, Florens L, Swanson S, Martin Brown S, Bradford W, et al. Characterization of Cullin-box sequences that direct recruitment of Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin ligases. J Biol Chem. 2008;283:8005-13 pubmed publisher..Taken together, our findings shed new light on the mechanisms by which BC-box proteins direct recruitment of Cullin-Rbx modules during reconstitution of ubiquitin ligases. ..
- Xiao H, Qian W, Staschke K, Qian Y, Cui G, Deng L, et al. Pellino 3b negatively regulates interleukin-1-induced TAK1-dependent NF kappaB activation. J Biol Chem. 2008;283:14654-64 pubmed publisher..Taken together, our results suggest that Pellino 3b acts as a negative regulator for IL-1 signaling by regulating IRAK degradation through its ubiquitin protein ligase activity. ..
- Brooks W, Helton E, Banerjee S, Venable M, Johnson L, Schoeb T, et al. G2E3 is a dual function ubiquitin ligase required for early embryonic development. J Biol Chem. 2008;283:22304-15 pubmed publisher..In adult animals, the most intense staining is found in Purkinje cell bodies and cells lining the ductus deferens. In summary, G2E3 is a dual function ubiquitin ligase essential for prevention of apoptosis in early embryogenesis. ..
- Schartner J, Singh A, Dahlberg P, Nettenstrom L, Seroogy C. Recurrent superantigen exposure in vivo leads to highly suppressive CD4+CD25+ and CD4+CD25- T cells with anergic and suppressive genetic signatures. Clin Exp Immunol. 2009;155:348-56 pubmed publisher..These data provide a novel paradigm for chronic non-canonical T cell receptor engagement leading to highly suppressive FoxP3(+)GRAIL(+)CD4(+) T cells. ..
- Kim Y, Francisco L, Chen G, Marcotte E, Chan C. Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol. 1994;127:1381-94 pubmed..These genetic interactions together suggest that protein phosphorylation and dephosphorylation play an important role in the BEM2-mediated process of polarized cell growth. ..
- Petrucelli L, O Farrell C, Lockhart P, Baptista M, Kehoe K, Vink L, et al. Parkin protects against the toxicity associated with mutant alpha-synuclein: proteasome dysfunction selectively affects catecholaminergic neurons. Neuron. 2002;36:1007-19 pubmed..Therefore, parkin and alpha-synuclein are linked by common effects on a pathway associated with selective cell death in catecholaminergic neurons. ..
- Feng L, Guedes S, Wang T. Atrophin-1-interacting protein 4/human Itch is a ubiquitin E3 ligase for human enhancer of filamentation 1 in transforming growth factor-beta signaling pathways. J Biol Chem. 2004;279:29681-90 pubmed..These findings reveal a new mechanism for Smad3-regulated proteasomal degradation events and also broaden the network of cross-talk between the TGF-beta signaling pathway and those involving HEF1 and AIP4. ..
- Wszolek Z, Pfeiffer R, Tsuboi Y, Uitti R, McComb R, Stoessl A, et al. Autosomal dominant parkinsonism associated with variable synuclein and tau pathology. Neurology. 2004;62:1619-22 pubmed..Genetic studies suggested linkage to the PARK8 locus on chromosome 12. ..