Genomes and Genes
Experts and Doctors on tertiary protein structure in Sweden
Topic: tertiary protein structure
Publications175 found, 100 shown here
- Zamyatnin A, Solovyev A, Bozhkov P, Valkonen J, Morozov S, Savenkov E. Assessment of the integral membrane protein topology in living cells. Plant J. 2006;46:145-54 pubmed
- Yang S, Berggren P. The role of voltage-gated calcium channels in pancreatic beta-cell physiology and pathophysiology. Endocr Rev. 2006;27:621-76 pubmed..This review summarizes current knowledge of CaV channels in beta-cell physiology and pathophysiology. ..
- Elmlund H, Baraznenok V, Lindahl M, Samuelsen C, Koeck P, Holmberg S, et al. The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II. Proc Natl Acad Sci U S A. 2006;103:15788-93 pubmed
- Chapman G, Liu L, Sahlgren C, Dahlqvist C, Lendahl U. High levels of Notch signaling down-regulate Numb and Numblike. J Cell Biol. 2006;175:535-40 pubmed..Collectively, these observations reveal a reciprocal negative regulation between Notch and Numb/Numblike, which may be of relevance for stabilizing asymmetric cell fate switches and for tumor development. ..
- Lundin B, Thuswaldner S, Shutova T, Eshaghi S, Samuelsson G, Barber J, et al. Subsequent events to GTP binding by the plant PsbO protein: structural changes, GTP hydrolysis and dissociation from the photosystem II complex. Biochim Biophys Acta. 2007;1767:500-8 pubmed..We propose the occurrence in higher plants of a PsbO-mediated GTPase activity associated with PSII, which has consequences for the function of the oxygen-evolving complex and D1 protein turnover. ..
- Sabri N, Roth P, Xylourgidis N, Sadeghifar F, Adler J, Samakovlis C. Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport. J Cell Biol. 2007;178:557-65 pubmed..Our data suggest that peripheral metazoan FG nucleoporins have distinct functions in nuclear protein transport events. ..
- Danfelter M, Onnerfjord P, Heinegard D. Fragmentation of proteins in cartilage treated with interleukin-1: specific cleavage of type IX collagen by matrix metalloproteinase 13 releases the NC4 domain. J Biol Chem. 2007;282:36933-41 pubmed..These data describe cleavage by an MMP-13 activity toward non-collagenous and triple helix domains. These potentially important degradation events precede the major loss of type II collagen. ..
- Dhanda R, Lindberg S, Olsson I. The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues. BMC Mol Biol. 2008;9:8 pubmed publisher..Our data from protein-protein interactions and immunolocalization experiments support that hSIN3B is a potential member of a corepressor complex involving selective ETO homologues. ..
- Burglin T. Evolution of hedgehog and hedgehog-related genes, their origin from Hog proteins in ancestral eukaryotes and discovery of a novel Hint motif. BMC Genomics. 2008;9:127 pubmed publisher..The Hog domain proteins, the inteins and VWA-Vint proteins are three families of Hint domain proteins that evolved in parallel in eukaryotes. ..
- Shaikhibrahim Z, Rahaman H, Wittung Stafshede P, Bjorklund S. Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation. Proc Natl Acad Sci U S A. 2009;106:20728-33 pubmed publisher..However, the concurrent presence of all three protein subunits during the renaturation process is required for proper folding and trimer complex formation. ..
- Dubnovitsky A, Duck Z, Kersley J, Härd T, MacIntyre S, Knight S. Conserved hydrophobic clusters on the surface of the Caf1A usher C-terminal domain are important for F1 antigen assembly. J Mol Biol. 2010;403:243-59 pubmed publisher..These clusters are conserved between the FGL family and the FGS family of chaperone-usher systems. ..
- Adén J, Wolf Watz M. NMR identification of transient complexes critical to adenylate kinase catalysis. J Am Chem Soc. 2007;129:14003-12 pubmed..On the basis of these and previous results, we propose that adenylate kinase belongs to a group of enzymes whose substrates act to shift pre-existing equilibria toward catalytically active states. ..
- Svensson C, Teneberg S, Nilsson C, Kjellberg A, Schwarz F, Sharon N, et al. High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data. J Mol Biol. 2002;321:69-83 pubmed
- Bladh L, Liden J, Pazirandeh A, Rafter I, Dahlman Wright K, Nilsson S, et al. Identification of target genes involved in the antiproliferative effect of glucocorticoids reveals a role for nuclear factor-(kappa)B repression. Mol Endocrinol. 2005;19:632-43 pubmed..These results demonstrate that GC-mediated inhibition of NF-(kappa)B is an important mechanism in the antiproliferative response to GCs. ..
- Gustafsson D, Segerman A, Lindman K, Mei Y, Wadell G. The Arg279Gln [corrected] substitution in the adenovirus type 11p (Ad11p) fiber knob abolishes EDTA-resistant binding to A549 and CHO-CD46 cells, converting the phenotype to that of Ad7p. J Virol. 2006;80:1897-905 pubmed..In conjunction with previous results, our findings also strongly suggest that sB2AR is equivalent to CD46...
- Wu S, Haag L, Sjöberg M, Garoff H, Hammar L. The dynamic envelope of a fusion class II virus. E3 domain of glycoprotein E2 precursor in Semliki Forest virus provides a unique contact with the fusion protein E1. J Biol Chem. 2008;283:26452-60 pubmed publisher..This finding reevaluates the role of the precursor from being only a provider of a shield over the fusion loop to a structural playmate in formation of the fusogenic architecture. ..
- Esser J, Rakonjac M, Hofmann B, Fischer L, Provost P, Schneider G, et al. Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102. Biochem J. 2009;425:265-74 pubmed publisher..The results of the present study support a role for CLP as a chaperoning scaffold factor, influencing both the stability and the activity of 5-LO. ..
- Sollerbrant K, Raschperger E, Mirza M, Engstrom U, Philipson L, Ljungdahl P, et al. The Coxsackievirus and adenovirus receptor (CAR) forms a complex with the PDZ domain-containing protein ligand-of-numb protein-X (LNX). J Biol Chem. 2003;278:7439-44 pubmed..CAR and LNX were also shown to colocalize in vivo in mammalian cells. We speculate that CAR and LNX are part of a larger protein complex that might have important functions at discrete subcellular localizations in the cell. ..
- Yamada S, Busse M, Ueno M, Kelly O, Skarnes W, Sugahara K, et al. Embryonic fibroblasts with a gene trap mutation in Ext1 produce short heparan sulfate chains. J Biol Chem. 2004;279:32134-41 pubmed..These data suggest that not only the sulfation pattern but also the length of the heparan sulfate chains is a critical determinant of normal mouse development. ..
- Turner P, Labes A, Fridjonsson O, Hreggvidson G, Schonheit P, Kristjansson J, et al. Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria have similar domain structures but differ in oligomeric state and activity profile. J Biosci Bioeng. 2005;100:380-90 pubmed
- Hashemy S, Holmgren A. Regulation of the catalytic activity and structure of human thioredoxin 1 via oxidation and S-nitrosylation of cysteine residues. J Biol Chem. 2008;283:21890-8 pubmed publisher..The reversible inhibition of human Trx1 activity by H(2)O(2) and NO donors is suggested to act in cell signaling via temporal control of reduction for the transmission of oxidative and/or nitrosative signals in thiol redox control. ..
- Mercer E, Korhonen L, Skoglösa Y, Olsson P, Kukkonen J, Lindholm D. NAIP interacts with hippocalcin and protects neurons against calcium-induced cell death through caspase-3-dependent and -independent pathways. EMBO J. 2000;19:3597-607 pubmed..Thus NAIP, in conjunction with hippocalcin, can protect neurons against calcium-induced cell death in caspase-3-activated and non-activated pathways. ..
- Sunnerhagen M, Olah G, Stenflo J, Forsen S, Drakenberg T, Trewhella J. The relative orientation of Gla and EGF domains in coagulation factor X is altered by Ca2+ binding to the first EGF domain. A combined NMR-small angle X-ray scattering study. Biochemistry. 1996;35:11547-59 pubmed..Biol. 2, 504-509]. Finally, our study clearly demonstrates the powerful combination of NMR and SAXS in the study of modular proteins, since this enables reliable evaluation of both short-range (NMR) and long-range interactions (SAXS). ..
- Pereira T, Zheng X, Ruas J, Tanimoto K, Poellinger L. Identification of residues critical for regulation of protein stability and the transactivation function of the hypoxia-inducible factor-1alpha by the von Hippel-Lindau tumor suppressor gene product. J Biol Chem. 2003;278:6816-23 pubmed..In conclusion, we have identified by mutation analysis several residues that are critical for either one or both of the interdigitated and conditionally regulated degradation and transactivation functions of the N-TAD of HIF-1alpha. ..
- Cervin C, Orho Melander M, Ridderstrale M, Lehto M, Barg S, Groop L, et al. Characterization of a naturally occurring mutation (L107I) in the HNF1 alpha (MODY3) gene. Diabetologia. 2002;45:1703-8 pubmed..Our results suggest that the L107I substitution represents a MODY3 mutation which impairs beta-cell function by a loss-of-function mechanism. ..
- Farnegardh M, Bonn T, Sun S, Ljunggren J, Ahola H, Wilhelmsson A, et al. The three-dimensional structure of the liver X receptor beta reveals a flexible ligand-binding pocket that can accommodate fundamentally different ligands. J Biol Chem. 2003;278:38821-8 pubmed..Both ligands induce a fixed "agonist conformation" of helix H12 (also called the AF-2 domain), resulting in a transcriptionally active receptor. ..
- Blom A, Villoutreix B, Dahlback B. Functions of human complement inhibitor C4b-binding protein in relation to its structure. Arch Immunol Ther Exp (Warsz). 2004;52:83-95 pubmed
- Thorsell A, Persson C, Voevodskaya N, Busam R, Hammarström M, Gräslund S, et al. Structural and biophysical characterization of human myo-inositol oxygenase. J Biol Chem. 2008;283:15209-16 pubmed publisher..Furthermore, based on site-directed mutagenesis in combination with biochemical and biophysical data, we propose a novel role for Lys(127) in governing access to the diiron cluster. ..
- Wai S, Westermark M, Oscarsson J, Jass J, Maier E, Benz R, et al. Characterization of dominantly negative mutant ClyA cytotoxin proteins in Escherichia coli. J Bacteriol. 2003;185:5491-9 pubmed..The observed protein-protein interactions were consistent with, and provided a molecular explanation for, the dominant negative feature of the mutant ClyA variants. ..
- Tanimoto K, Makino Y, Pereira T, Poellinger L. Mechanism of regulation of the hypoxia-inducible factor-1 alpha by the von Hippel-Lindau tumor suppressor protein. EMBO J. 2000;19:4298-309 pubmed..VHL was not released from HIF-1 alpha during this process. Finally, stabilization of HIF-1 alpha protein levels per se did not totally bypass the need of the hypoxic signal for generating the transactivation response. ..
- Bill R, Hedfalk K, Karlgren S, Mullins J, Rydstrom J, Hohmann S. Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1p. J Biol Chem. 2001;276:36543-9 pubmed..We discuss the fact that this may accommodate the divergent NPA motifs of Fps1p and that the different pore structures of Fps1p and GlpF may reflect the physiological roles of the two glycerol facilitators. ..
- Narváez A, Voevodskaya N, Thelander L, Gräslund A. The involvement of Arg265 of mouse ribonucleotide reductase R2 protein in proton transfer and catalysis. J Biol Chem. 2006;281:26022-8 pubmed..Our results, supported by molecular modeling, strongly suggest that Arg265 is involved in the proton-coupled electron transfer pathway and may act as a proton mediator during catalysis. ..
- Khan F, Spicarova Z, Zelenin S, Holtback U, Scott L, Aperia A. Negative reciprocity between angiotensin II type 1 and dopamine D1 receptors in rat renal proximal tubule cells. Am J Physiol Renal Physiol. 2008;295:F1110-6 pubmed publisher..Results indicate that AT1R and D1R function as a unit of opposites, which should provide a highly versatile and sensitive system for short-term regulation of sodium excretion. ..
- Abrescia C, Sjöstrand D, Kjaer S, Ibanez C. Drosophila RET contains an active tyrosine kinase and elicits neurotrophic activities in mammalian cells. FEBS Lett. 2005;579:3789-96 pubmed..These results indicate significant conservation between the biological effects elicited by the human and Drosophila RET kinases, and suggest functions for dRET in neuronal differentiation in the fly. ..
- Lindberg U, Schutt C, Goldman R, Nyåkern Meazza M, Hillberg L, Rathje L, et al. Tropomyosins regulate the impact of actin binding proteins on actin filaments. Adv Exp Med Biol. 2008;644:223-31 pubmed..The existence of these recyclable, tropomyosin isoform-specific structures suggests how cells prevent nonproductive association of non-identical, but closely similar, tropomyosin isoforms. ..
- Gullberg D, Lundgren Akerlund E. Collagen-binding I domain integrins--what do they do?. Prog Histochem Cytochem. 2002;37:3-54 pubmed..In this review we will describe what is currently known about the collagen-binding integrins and discuss their biological functions. ..
- Rapp M, Seppälä S, Granseth E, von Heijne G. Emulating membrane protein evolution by rational design. Science. 2007;315:1282-4 pubmed..This simple evolutionary pathway can explain the frequent occurrence of membrane proteins with an internal pseudo-two-fold symmetry axis in the plane of the membrane. ..
- Johansson D, Macao B, Sandberg A, Härd T. SEA domain autoproteolysis accelerated by conformational strain: mechanistic aspects. J Mol Biol. 2008;377:1130-43 pubmed publisher..The energetics of this coupled folding and autoproteolysis mechanism is accounted for in an accompanying article. ..
- Wanngren J, Frånberg J, Svensson A, Laudon H, Olsson F, Winblad B, et al. The large hydrophilic loop of presenilin 1 is important for regulating gamma-secretase complex assembly and dictating the amyloid beta peptide (Abeta) Profile without affecting Notch processing. J Biol Chem. 2010;285:8527-36 pubmed publisher
- Ahnström J, Faber K, Axler O, Dahlback B. Hydrophobic ligand binding properties of the human lipocalin apolipoprotein M. J Lipid Res. 2007;48:1754-62 pubmed..In conclusion, the binding of retinol and retinoic acid supports the hypothesis that apoM is a lipocalin. The physiological relevance of this binding has yet to be elucidated. ..
- Krajewski W, Collins R, Holmberg Schiavone L, Jones T, Karlberg T, Mowbray S. Crystal structures of mammalian glutamine synthetases illustrate substrate-induced conformational changes and provide opportunities for drug and herbicide design. J Mol Biol. 2008;375:217-28 pubmed..Thus, the latter site offers the best target for specific drug design. Differences between mammalian and plant enzymes are much more subtle, suggesting that herbicides targeting GS must be designed with caution. ..
- Nilsson S, Christensen S, Raarup M, Ryan R, Nielsen M, Olivecrona G. Endocytosis of apolipoprotein A-V by members of the low density lipoprotein receptor and the VPS10p domain receptor families. J Biol Chem. 2008;283:25920-7 pubmed publisher..We conclude that apoA-V binds to receptors possessing LDL-A repeats and Vsp10p domains and that apoA-V is internalized into cells via these receptors. This could be a mechanism by which apoA-V modulates lipoprotein metabolism in vivo. ..
- Onions J, Hermann S, Grundstrom T. A novel type of calmodulin interaction in the inhibition of basic helix-loop-helix transcription factors. Biochemistry. 2000;39:4366-74 pubmed
- Katona G, Wilmouth R, Wright P, Berglund G, Hajdu J, Neutze R, et al. X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution. J Biol Chem. 2002;277:21962-70 pubmed
- Bizouarn T, Fjellström O, Axelsson M, Korneenko T, Pestov N, Ivanova M, et al. Interactions between the soluble domain I of nicotinamide nucleotide transhydrogenase from Rhodospirillum rubrum and transhydrogenase from Escherichia coli. Effects on catalytic and H+-pumping activities. Eur J Biochem. 2000;267:3281-8 pubmed..The reverse reaction and proton pumping showed a less pronounced change in pH dependence, demonstrating the regulatory role of domain II in these reactions. ..
- Enquist K, Fransson M, Boekel C, Bengtsson I, Geiger K, Lang L, et al. Membrane-integration characteristics of two ABC transporters, CFTR and P-glycoprotein. J Mol Biol. 2009;387:1153-64 pubmed publisher..Membrane-insertion characteristics can thus vary widely between related proteins...
- Bylund J, Björstad A, Granfeldt D, Karlsson A, Woschnagg C, Dahlgren C. Reactivation of formyl peptide receptors triggers the neutrophil NADPH-oxidase but not a transient rise in intracellular calcium. J Biol Chem. 2003;278:30578-86 pubmed..Further, phosphatidylinositol 3-kinase, mitogen-activated protein kinase, and MEK all participated in the generation of second messengers from the reactivated receptors. ..
- Lennartsson A, Pieters K, Vidovic K, Gullberg U. A murine antibacterial ortholog to human bactericidal/permeability-increasing protein (BPI) is expressed in testis, epididymis, and bone marrow. J Leukoc Biol. 2005;77:369-77 pubmed..In conclusion, it was demonstrated that mouse neutrophils store BPI with antibacterial activity and that murine BPI is also expressed in testis and epididymis. ..
- Tan No K, Cebers G, Yakovleva T, Hoon Goh B, Gileva I, Reznikov K, et al. Cytotoxic effects of dynorphins through nonopioid intracellular mechanisms. Exp Cell Res. 2001;269:54-63 pubmed..These effects may be relevant for a pathophysiological role of dynorphins in the brain and spinal cord and for control of death of tumor cells, which express prodynorphin at high levels. ..
- Sadek C, Damdimopoulos A, Pelto Huikko M, Gustafsson J, Spyrou G, Miranda Vizuete A. Sptrx-2, a fusion protein composed of one thioredoxin and three tandemly repeated NDP-kinase domains is expressed in human testis germ cells. Genes Cells. 2001;6:1077-90 pubmed
- Sauer Eriksson A, Hainzl T. S-domain assembly of the signal recognition particle. Curr Opin Struct Biol. 2003;13:64-70 pubmed
- Uramoto H, Hackzell A, Wetterskog D, Ballági A, Izumi H, Funa K. pRb, Myc and p53 are critically involved in SV40 large T antigen repression of PDGF beta-receptor transcription. J Cell Sci. 2004;117:3855-65 pubmed..However, LT binds the promoter in the absence of pRb and p53 in Saos-2 cells stably expressing LT. These results suggest that LT binds the promoter and interferes with NF-Y and Sp1 to repress it in the presence of Myc, pRb and p53. ..
- Henriksson L, Björkelid C, Mowbray S, Unge T. The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target. Acta Crystallogr D Biol Crystallogr. 2006;62:807-13 pubmed..Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis. ..
- Di Y, Tamás M. Regulation of the arsenic-responsive transcription factor Yap8p involves the ubiquitin-proteasome pathway. J Cell Sci. 2007;120:256-64 pubmed..In this way, regulated degradation contributes to Yap8p control. ..
- Almqvist J, Huang Y, Laaksonen A, Wang D, Hovmöller S. Docking and homology modeling explain inhibition of the human vesicular glutamate transporters. Protein Sci. 2007;16:1819-29 pubmed..Docking of VGLUT1 substrates to this pore localizes two different binding sites, to which inhibitors also bind with an overall trend in binding affinity that is in agreement with previously published experimental data. ..
- Lindås A, Eriksson S, Jozsa E, Tomkinson B. Investigation of a role for Glu-331 and Glu-305 in substrate binding of tripeptidyl-peptidase II. Biochim Biophys Acta. 2008;1784:1899-907 pubmed publisher..The present work is, to our knowledge, the first report to investigate a mechanism for a tripeptidyl-peptidase activity through site-directed mutagenesis. ..
- Mouillon J, Eriksson S, Harryson P. Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse. Plant Physiol. 2008;148:1925-37 pubmed publisher..These results suggest that the dehydrins are highly specialized proteins that have evolved to maintain their disordered character under conditions in which unfolded states of several globular proteins would tend to collapse. ..
- Danielsson J, Liljedahl L, Bárány Wallje E, Sønderby P, Kristensen L, Martinez Yamout M, et al. The intrinsically disordered RNR inhibitor Sml1 is a dynamic dimer. Biochemistry. 2008;47:13428-37 pubmed..We propose that this molecular shielding and protection of vital functional structures from degradation by functionally unimportant sites may be a general attribute of other natively disordered proteins. ..
- Kyriakopoulou C, Nordvarg H, Virtanen A. A novel nuclear human poly(A) polymerase (PAP), PAP gamma. J Biol Chem. 2001;276:33504-11 pubmed..The exclusive nuclear localization of hsPAP gamma establishes that multiple forms of PAP are unevenly distributed in the cell, implying specialized roles for the various isoforms. ..
- Waldenström M, Achour A, Michaelsson J, Rolle A, Karre K. The role of an exposed loop in the alpha2 domain in the mouse MHC class I H-2Dd molecule for recognition by the monoclonal antibody 34-5-8S and the NK-cell receptor Ly49A. Scand J Immunol. 2002;55:129-39 pubmed..These results demonstrate that the introduced changes at positions 103, 104 and 107 directly or indirectly affect the epitopes for the MoAb 34-5-8S and the Ly49A receptor. ..
- Berglund J, Knight S. Structural basis for bacterial adhesion in the urinary tract. Adv Exp Med Biol. 2003;535:33-52 pubmed
- Persson C, Oldenvi S, Steiner H. Peptidoglycan recognition protein LF: a negative regulator of Drosophila immunity. Insect Biochem Mol Biol. 2007;37:1309-16 pubmed..Furthermore, following immune stimulation of S2 cells overexpressing PGRP-LF, we noticed a reduced up-regulation of expression of antimicrobial peptide genes, in consonance with an immune suppressive role for PGRP-LF. ..
- Romling U. Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea. Sci Signal. 2008;1:pe39 pubmed publisher..To elucidate the biological importance and molecular mechanisms of action for c-di-AMP and the recently recognized second messenger c-di-GMP will require a multidisciplinary approach. ..
- Pacheco B, Maccarana M, Goodlett D, Malmstrom A, Malmström L. Identification of the active site of DS-epimerase 1 and requirement of N-glycosylation for enzyme function. J Biol Chem. 2009;284:1741-7 pubmed publisher..In addition, we show that proper N-glycosylation of DS-epimerase 1 is required for enzyme activity. This study represents the first description of the structural basis for epimerization by a glycosaminoglycan epimerase. ..
- Schneider G, Lindqvist Y. Crystallography and mutagenesis of transketolase: mechanistic implications for enzymatic thiamin catalysis. Biochim Biophys Acta. 1998;1385:387-98 pubmed..Based on this structural information, the function of various active site residues in substrate binding and catalysis has been probed by site-directed mutagenesis. ..
- Lidell M, Johansson M, Hansson G. An autocatalytic cleavage in the C terminus of the human MUC2 mucin occurs at the low pH of the late secretory pathway. J Biol Chem. 2003;278:13944-51 pubmed..Because a pH below 6 can be reached in the late secretory pathway and on mucosal surfaces, the cleavage and possible cross-linking are likely to be of biological importance. ..
- Heathfield T, Onnerfjord P, Dahlberg L, Heinegard D. Cleavage of fibromodulin in cartilage explants involves removal of the N-terminal tyrosine sulfate-rich region by proteolysis at a site that is sensitive to matrix metalloproteinase-13. J Biol Chem. 2004;279:6286-95 pubmed
- Seppälä S, Slusky J, Lloris Garcerá P, Rapp M, von Heijne G. Control of membrane protein topology by a single C-terminal residue. Science. 2010;328:1698-700 pubmed publisher..This observation points to an unanticipated plasticity in membrane protein insertion mechanisms. ..
- Golmohammadi R, Fridborg K, Bundule M, Valegård K, Liljas L. The crystal structure of bacteriophage Q beta at 3.5 A resolution. Structure. 1996;4:543-54 pubmed..We have determined the crystal structure of phage Q beta with the aim of clarifying which factors are the most important for particle assembly and RNA interaction in the small phages...
- Izumi H, Molander C, Penn L, Ishisaki A, Kohno K, Funa K. Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor. J Cell Sci. 2001;114:1533-44 pubmed..Furthermore, this repression was seen only when Myc homology boxes are present, and NF-YC contains the c-Myc binding region. ..
- Linhult M, Gülich S, Gräslund T, Nygren P, Hober S. Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand. Protein Eng. 2003;16:1147-52 pubmed
- Eriksson T, Stals I, Collén A, Tjerneld F, Claeyssens M, Stålbrand H, et al. Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei) Cel7B catalytic module. Eur J Biochem. 2004;271:1266-76 pubmed..However, partial deamidation of Asn259 and a partially occupied O-glycosylation site give rise to further complexity in enzyme preparations. ..
- Lindahl M, Irander K, Tagesson C, Ståhlbom B. Nasal lavage fluid and proteomics as means to identify the effects of the irritating epoxy chemical dimethylbenzylamine. Biomarkers. 2004;9:56-70 pubmed..Further studies are needed to investigate whether these proteins may be used as biomarkers of airway irritation and to give new insight into the ways in which occupational exposure to irritants causes inflammation of the airways...
- Marklund M, Sjödal M, Beehler B, Jessell T, Edlund T, Gunhaga L. Retinoic acid signalling specifies intermediate character in the developing telencephalon. Development. 2004;131:4323-32 pubmed..We provide evidence here that retinoic acid has a crucial role in specifying telencephalic progenitor cells of intermediate character. ..
- Söderberg L, Kakuyama H, Möller A, Ito A, Winblad B, Tjernberg L, et al. Characterization of the Alzheimer's disease-associated CLAC protein and identification of an amyloid beta-peptide-binding site. J Biol Chem. 2005;280:1007-15 pubmed..These findings may be useful for future therapeutic interventions aimed at finding compounds that modulate the binding of CLAC to Abeta deposits. ..
- Axelsson F, Adler S, Lamarre A, Ohlin M. Humoral immunity targeting site I of antigenic domain 2 of glycoprotein B upon immunization with different cytomegalovirus candidate vaccines. Vaccine. 2007;26:41-6 pubmed..As in natural infection only a fraction of all subjects developed antibody responses against site I of AD-2 following vaccination. We suggest that strategies that enhance immunogenicity of this epitope will improve vaccine efficacy. ..
- Nyman U, Vlachos P, Cascante A, Hermanson O, Zhivotovsky B, Joseph B. Protein kinase C-dependent phosphorylation regulates the cell cycle-inhibitory function of the p73 carboxy terminus transactivation domain. Mol Cell Biol. 2009;29:1814-25 pubmed publisher..Our results suggest that this novel posttranslational modification within the p73 carboxy terminus transactivation domain is involved in the context-specific guidance of p73 toward the selective induction of cell cycle arrest. ..
- Papadopoulos E, Billeter M, Gräslund A, Vlamis Gardikas A. Assignment of (1)H, (13)C, and (15)N resonances of YgiT, a putative DNA interacting protein from E. coli, containing one HTH and two CxxC motifs. Biomol NMR Assign. 2007;1:217-9 pubmed publisher..We report the backbone and side chain (1)H, (13)C, and (15)N resonances assignment of YgiT. ..
- Gustavsson M, Lehtiö J, Denman S, Teeri T, Hult K, Martinelle M. Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris. Protein Eng. 2001;14:711-5 pubmed..The lipase-specific activities of all variants, in solution or immobilized on to cellulose, were equal to that of the wild-type lipase...
- Hafizi S, Alindri F, Karlsson R, Dahlback B. Interaction of Axl receptor tyrosine kinase with C1-TEN, a novel C1 domain-containing protein with homology to tensin. Biochem Biophys Res Commun. 2002;299:793-800 pubmed..In summary, we have identified a novel multi-domain intracellular protein that interacts with Axl and which may furthermore be involved in other signal transduction pathways. ..
- Aifa S, Aydin J, Nordvall G, Lundstrom I, Svensson S, Hermanson O. A basic peptide within the juxtamembrane region is required for EGF receptor dimerization. Exp Cell Res. 2005;302:108-14 pubmed
- Johansson T, Oswald C, Pedersen A, Törnroth S, Okvist M, Karlsson B, et al. X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli. J Mol Biol. 2005;352:299-312 pubmed..Taken together, these results have important implications for the enzyme mechanism of the large group of transhydrogenases, including mammalian enzymes, which contain a connecting linker between domains I and II. ..
- Swahn B, Huerta F, Kallin E, Malmström J, Weigelt T, Viklund J, et al. Design and synthesis of 6-anilinoindazoles as selective inhibitors of c-Jun N-terminal kinase-3. Bioorg Med Chem Lett. 2005;15:5095-9 pubmed..The novel series of substituted 6-anilinoindazoles were designed based on a combination of hits from high throughput screening and X-ray crystal structure information of the compounds crystallized into the JNK3 ATP binding active site. ..
- Andér M, Aqvist J. Does glutamine methylation affect the intrinsic conformation of the universally conserved GGQ motif in ribosomal release factors?. Biochemistry. 2009;48:3483-9 pubmed publisher..The results show that there is no such structural effect on the free release factor caused by the Nepsilon methylation of Q185, suggesting that its role is intimately associated with the ribosome environment. ..
- Althage M, Bizouarn T, Rydstrom J. Identification of a region involved in the communication between the NADP(H) binding domain and the membrane domain in proton pumping E. coli transhydrogenase. Biochemistry. 2001;40:9968-76 pubmed
- Hulkko S, Zilliacus J. Functional interaction between the pro-apoptotic DAP3 and the glucocorticoid receptor. Biochem Biophys Res Commun. 2002;295:749-55 pubmed..Since full-length DAP3 is also necessary for the pro-apoptotic effect, the interplay between the N- and C-termini of DAP3 is probably essential for its cellular function. ..
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- Monné M, Han L, Schwend T, Burendahl S, Jovine L. Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats. Nature. 2008;456:653-7 pubmed publisher..Moreover, it provides an important framework for understanding human diseases caused by mutations in ZP domain proteins and developing new methods of non-hormonal contraception. ..
- Hafizi S, Sernstad E, Swinny J, Gomez M, Dahlback B. Individual domains of Tensin2 exhibit distinct subcellular localisations and migratory effects. Int J Biochem Cell Biol. 2010;42:52-61 pubmed publisher..In conclusion, our results indicate that differential proteolytic cleavage of Tensin2 can liberate domains with discrete localisations and functions, which has implications for the role of Tensins in cancer cell survival and motility. ..