Experts and Doctors on spliceosomes in Taiwan


Locale: Taiwan
Topic: spliceosomes

Top Publications

  1. Tarn W, Hsu C, Huang K, Chen H, Kao H, Lee K, et al. Functional association of essential splicing factor(s) with PRP19 in a protein complex. EMBO J. 1994;13:2421-31 pubmed
    ..At least three of these can interact directly with the PRP19 protein. We also show that the PRP19 protein itself is in an oligomeric form, which might be a prerequisite for its interaction with these proteins. ..
  2. Lin K, Lu R, Tarn W. The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo. Mol Cell Biol. 2004;24:9176-85 pubmed
    ..Our results suggest an essential role of WW/FF domain-containing factors in pre-mRNA splicing that likely occurs in concert with transcription in vivo. ..
  3. Tsai R, Fu R, Yeh F, Tseng C, Lin Y, Huang Y, et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev. 2005;19:2991-3003 pubmed
    ..This is the first demonstration of physical disassembly of the spliceosome, catalyzed by a complex containing a DExD/H-box RNA helicase and two accessory factors, which might function in targeting the helicase to the correct substrate. ..
  4. Tsai R, Tseng C, Lee P, Chen H, Fu R, Chang K, et al. Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly. Mol Cell Biol. 2007;27:8027-37 pubmed
    ..Our results demonstrate that dynamic interactions of NTR with U5, through the interaction of Ntr2 with Brr2, and interactions of Ntr1 and Prp43 govern the recruitment of Prp43 to the spliceosome to mediate spliceosome disassembly. ..
  5. Chiu Y, Liu Y, Chiang T, Yeh T, Tseng C, Wu N, et al. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol Cell Biol. 2009;29:5671-8 pubmed publisher
    ..These results have implications for the possible roles of Cwc25 and HP-X in facilitating juxtaposition of the 5' splice site and the branch point during the first catalytic reaction...
  6. Yeh T, Liu H, Chung C, Wu N, Liu Y, Cheng S. Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway. Mol Cell Biol. 2011;31:43-53 pubmed publisher
    ..Thus, Cwc22 represents a novel ATP-dependent step one factor besides Prp2 and Spp2 and has a distinct role from that of Spp2 in mediating the function of Prp2...
  7. Tseng C, Liu H, Cheng S. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. RNA. 2011;17:145-54 pubmed publisher
    ..Our results uncovered novel functions of Prp16 in both catalytic steps, and provide mechanistic insights into splicing catalysis...
  8. Chuang T, Peng P, Tarn W. The exon junction complex component Y14 modulates the activity of the methylosome in biogenesis of spliceosomal small nuclear ribonucleoproteins. J Biol Chem. 2011;286:8722-8 pubmed publisher
    ..We hypothesize that Y14 provides a regulatory link between pre-mRNA splicing and snRNP biogenesis. ..
  9. Liu H, Cheng S. The interaction of Prp2 with a defined region of the intron is required for the first splicing reaction. Mol Cell Biol. 2012;32:5056-66 pubmed publisher

More Information


  1. Chen H, Tseng C, Tsai R, Chung C, Cheng S. Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22. Mol Cell Biol. 2013;33:514-25 pubmed publisher
  2. Chiang T, Cheng S. A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing. Mol Cell Biol. 2013;33:1746-55 pubmed publisher
  3. Liu Y, Chen H, Wu N, Cheng S. A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol Cell Biol. 2007;27:5403-13 pubmed
  4. Chang K, Chen H, Cheng S. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA. 2009;15:1729-39 pubmed publisher
    ..Our results demonstrate a novel role of the NTC in recruiting splicing factors to the spliceosome after its activation. ..