Experts and Doctors on sensitivity and specificity in Finland


Locale: Finland
Topic: sensitivity and specificity

Top Publications

  1. Soukka T, Paukkunen J, Harma H, Lönnberg S, Lindroos H, Lovgren T. Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem. 2001;47:1269-78 pubmed
  2. Flinkkilä T, Ristiniemi J, Hyvönen P, Hämäläinen M. Surgical treatment of unstable fractures of the distal clavicle: a comparative study of Kirschner wire and clavicular hook plate fixation. Acta Orthop Scand. 2002;73:50-3 pubmed
    ..The clavicular hook plate was better in this respect and it is therefore recommended. ..
  3. Torkki M, Malmivaara A, Reivonen N, Seitsalo S, Laippalo P, Hoikka V. Individually fitted sports shoes for overuse injuries among newspaper carriers. Scand J Work Environ Health. 2002;28:176-83 pubmed
    ..Individually adjusted shock-absorbing shoes offer slight health benefits for lower-limb overuse injuries. Proper shoes may decrease the need to use health care resources. ..
  4. Siggins S, Kärkkäinen M, Tenhunen J, Metso J, Tahvanainen E, Olkkonen V, et al. Quantitation of the active and low-active forms of human plasma phospholipid transfer protein by ELISA. J Lipid Res. 2004;45:387-95 pubmed
    ..The HA-PLTP mass (mean, 1.87 +/- 0.85 mg/l) correlated significantly with serum PLTP activity, whereas that of LA-PLTP (mean, 3.94 +/- 1.4 mg/l) showed no correlation with phospholipid transfer activity. ..
  5. Peippo P, Hagren V, Lövgren T, Tuomola M. Rapid time-resolved fluoroimmunoassay for the screening of narasin and salinomycin residues in poultry and eggs. J Agric Food Chem. 2004;52:1824-8 pubmed
    ..1 and 6.4%, respectively. The CVs of intra-assay precision tests, determined by 10 replicate analyses at the above-mentioned concentration levels, were 3.8 and 4.5%, respectively. ..
  6. Toppila Salmi S, Myller J, Torkkeli T, Muhonen J, Renkonen J, Rautiainen M, et al. Endothelial L-selectin ligands in sinus mucosa during chronic maxillary rhinosinusitis. Am J Respir Crit Care Med. 2005;171:1350-7 pubmed
  7. Ala Korpela M, Lankinen N, Salminen A, Suna T, Soininen P, Laatikainen R, et al. The inherent accuracy of 1H NMR spectroscopy to quantify plasma lipoproteins is subclass dependent. Atherosclerosis. 2007;190:352-8 pubmed
    ..This finding has critical significance since the usage of (1)H NMR methodology in the clinical arena is rapidly increasing. ..
  8. Koskinen M, Vartiainen N. Removal of ballistocardiogram artifact from EEG data acquired in the MRI scanner: selection of ICA components. Conf Proc IEEE Eng Med Biol Soc. 2008;2008:5220-3 pubmed publisher
    ..The suggested methods may provide objective means to validate residual artifact or the possible loss of physiological signal due to artifact removal and to help selecting the proper artifact-related components. ..
  9. Syed P, Gidwani K, Kekki H, Leivo J, Pettersson K, Lamminmaki U. Role of lectin microarrays in cancer diagnosis. Proteomics. 2016;16:1257-65 pubmed publisher

More Information

Publications109 found, 100 shown here

  1. Piironen T, Lovgren J, Karp M, Eerola R, Lundwall A, Dowell B, et al. Immunofluorometric assay for sensitive and specific measurement of human prostatic glandular kallikrein (hK2) in serum. Clin Chem. 1996;42:1034-41 pubmed
    ..Gel filtration of 10 serum samples with increased hK2 concentrations showed a single peak of hK2 immunoreactivity with an apparent molecular size of approximately 30 kDa, corresponding to that of recombinant hK2 and free PSA. ..
  2. Färkkilä M, Kairemo K, Taavitsainen M, Strandberg T, Miettinen T. Plasma lathosterol as a screening test for bile acid malabsorption due to ileal resection: correlation with 75SeHCAT test and faecal bile acid excretion. Clin Sci (Lond). 1996;90:315-9 pubmed
    ..4. The results indicate that both the 75SeHCAT test and plasma lathosterol detect bile acid malabsorption in patients with ileal resections for Crohn's disease. However, plasma lathosterol is a simpler and less expensive method. ..
  3. Kallio Kokko H, Lundkvist A, Plyusnin A, Avsic Zupanc T, Vaheri A, Vapalahti O. Antigenic properties and diagnostic potential of recombinant dobrava virus nucleocapsid protein. J Med Virol. 2000;61:266-74 pubmed
    ..The specificity values were 100% for bac-DOBV-N and 99% for DOBV-dN-GST by IgM assays, and 100% for both antigens by IgG assays. ..
  4. Kurittu J, Karp M, Korpela M. Detection of tetracyclines with luminescent bacterial strains. Luminescence. 2000;15:291-7 pubmed
    ..The preliminary results from the analysis of tetracycline-spiked pork serum samples indicated that these sensor bacteria could be used to screen veterinary samples for tetracycline residues in real-time. ..
  5. Tarvainen T, Kolehmainen V, Vauhkonen M, Vanne A, Gibson A, Schweiger M, et al. Computational calibration method for optical tomography. Appl Opt. 2005;44:1879-88 pubmed
    ..The results show that the coupling coefficients of the sources and detectors can be estimated with good accuracy. Furthermore, the results show that the method can significantly improve the quality of reconstructed images. ..
  6. Kauhanen L, Nykopp T, Lehtonen J, Jylänki P, Heikkonen J, Rantanen P, et al. EEG and MEG brain-computer interface for tetraplegic patients. IEEE Trans Neural Syst Rehabil Eng. 2006;14:190-3 pubmed
    ..Classification accuracies obtained with dynamic classifier were better, at 75%, 89%, and 91% in different subjects, when features were in the 0.5-3.0-Hz frequency band. Classification accuracies of EEG and MEG did not differ. ..
  7. Kauhanen S, Seppanen M, Ovaska J, Minn H, Bergman J, Korsoff P, et al. The clinical value of [18F]fluoro-dihydroxyphenylalanine positron emission tomography in primary diagnosis, staging, and restaging of neuroendocrine tumors. Endocr Relat Cancer. 2009;16:255-65 pubmed publisher
    ..Both in primary diagnosis and in patients with formerly known NET and increasing tumor markers, [(18)F]DOPA PET-CT is a sensitive first-line imaging method. ..
  8. Linko A, Parikka K, Wahala K, Adlercreutz H. Gas chromatographic-mass spectrometric method for the determination of alkylresorcinols in human plasma. Anal Biochem. 2002;308:307-13 pubmed
    ..The alkylresorcinols were in the unconjugated form. The total amounts of alkylresorcinols in two plasma samples analyzed here were 333 and 381nmol/L. ..
  9. Mustonen A, Koivikko M, Haapamaki V, Kiuru M, Lamminen A, Koskinen S. Multidetector computed tomography in acute knee injuries: assessment of cruciate ligaments with magnetic resonance imaging correlation. Acta Radiol. 2007;48:104-11 pubmed
    ..MDCT can detect an intact ACL and PCL with good specificity, accuracy, and negative predictive value. The assessment of torn ligaments is unreliable. ..
  10. Jansson V, Jansson K. A continuous fluorometric assay for inorganic pyrophosphate and for detecting single-base changes with polymerase chain reaction. Anal Biochem. 2003;317:268-70 pubmed
  11. Harju T, Kaarteenaho Wiik R, Soini Y, Sormunen R, Kinnula V. Diminished immunoreactivity of gamma-glutamylcysteine synthetase in the airways of smokers' lung. Am J Respir Crit Care Med. 2002;166:754-9 pubmed
    ..Their tendency to decrease in cigarette smokers may further predispose lung cells to ongoing oxidant stress, which contributes to the progression of lung injury. ..
  12. Brouwers E, L homme R, Al Maharik N, Lapcik O, Hampl R, Wahala K, et al. Time-resolved fluoroimmunoassay for equol in plasma and urine. J Steroid Biochem Mol Biol. 2003;84:577-88 pubmed
    ..5-6.5 and 3.4-6.9, respectively. The inter-assay CV% varies between 5.4-9.7 and 7.4-7.7, respectively. The working ranges of the plasma and urine assay are 1.27-512 and 1.9-512nmol/l, respectively. ..
  13. Hirvonen J, Aalto S, Lumme V, NAgren K, Kajander J, Vilkman H, et al. Measurement of striatal and thalamic dopamine D2 receptor binding with 11C-raclopride. Nucl Med Commun. 2003;24:1207-14 pubmed
    ..However, the signal-to-noise ratio for quantification may become too low especially in receptor occupancy-type studies, leading to an artefactual underestimation of measured D2 receptor occupancy. ..
  14. Kettunen E, Salmenkivi K, Vuopala K, Toljamo T, Kuosma E, Norppa H, et al. Copy number gains on 5p15, 6p11-q11, 7p12, and 8q24 are rare in sputum cells of individuals at high risk of lung cancer. Lung Cancer. 2006;54:169-76 pubmed
    ..Significant differences were not found between sputa of asbestos-exposed individuals, heavy-smokers and never-smokers. More sensitive methods are needed for the follow-up of populations at high risk of contracting lung cancer. ..
  15. Haiminen N, Mannila H, Terzi E. Determining significance of pairwise co-occurrences of events in bursty sequences. BMC Bioinformatics. 2008;9:336 pubmed publisher
    ..More sophisticated models give better results in our experiments. We also demonstrate the effect of the window size, i.e., maximum co-occurrence distance, on the significance results. ..
  16. Kaikkonen L, Soderlund Venermo M, Brunstein J, Schou O, Panum Jensen I, Rousseau S, et al. Diagnosis of human parvovirus B19 infections by detection of epitope-type-specific VP2 IgG. J Med Virol. 2001;64:360-5 pubmed
    ..The 2nd-generation epitope-type EIA had a diagnostic sensitivity of 98% and a diagnostic specificity of 94%. In combination with conventional approaches, the epitope-type assays increase greatly the accuracy of B19 serodiagnosis. ..
  17. Saukkoriipi A, Leskelä K, Herva E, Leinonen M. Streptococcus pneumoniae in nasopharyngeal secretions of healthy children: comparison of real-time PCR and culture from STGG-transport medium. Mol Cell Probes. 2004;18:147-53 pubmed
    ..Quantitative real-time PCR provides feasible means for quantifying pneumococcal carriage. Further studies are needed to confirm that positive PCR findings really indicate the presence of viable pneumococcus in nasopharyngeal specimens. ..
  18. Koskela A, Samaletdin A, Aubertin Leheudre M, Adlercreutz H. Quantification of alkylresorcinol metabolites in plasma by high-performance liquid chromatography with coulometric electrode array detection. J Agric Food Chem. 2008;56:7678-81 pubmed publisher
    ..Additional investigation is needed to clarify whether the two plasma AR metabolites are useful as biomarkers of whole-grain intake and helpful in the exploration of the association between whole-grain cereal intake and human diseases. ..
  19. Risteli L, Risteli J. Biochemical markers of bone metabolism. Ann Med. 1993;25:385-93 pubmed
    ..We also attempt to relate the various collagenous and non-collagenous markers to each other and to the phases of the osteoblast phenotype. ..
  20. Mäntylä P, Stenman M, Kinane D, Tikanoja S, Luoto H, Salo T, et al. Gingival crevicular fluid collagenase-2 (MMP-8) test stick for chair-side monitoring of periodontitis. J Periodontal Res. 2003;38:436-9 pubmed
    ..A reduction in GCF MMP-8 levels and a change in test stick result provide a means to optimize patient control during maintenance of periodontal treatment. ..
  21. Myllykoski J, Nevas M, Lindstrom M, Korkeala H. The detection and prevalence of Clostridium botulinum in pig intestinal samples. Int J Food Microbiol. 2006;110:172-7 pubmed
    ..C. botulinum was isolated from two PCR-positive samples and confirmed to be type B by PCR. Both isolates were shown to be proteolytic and thus to represent C. botulinum group I. ..
  22. Elo L, Hiissa J, Tuimala J, Kallio A, Korpelainen E, Aittokallio T. Optimized detection of differential expression in global profiling experiments: case studies in clinical transcriptomic and quantitative proteomic datasets. Brief Bioinform. 2009;10:547-55 pubmed publisher
    ..To promote its widespread usage, we have made freely available efficient implementations of ROTS, which are easily accessible either as a stand-alone R-package or as integrated in the open-source data analysis software Chipster. ..
  23. Päkkilä H, Ylihärsilä M, Lahtinen S, Hattara L, Salminen N, Arppe R, et al. Quantitative multianalyte microarray immunoassay utilizing upconverting phosphor technology. Anal Chem. 2012;84:8628-34 pubmed publisher
    ..Multiplexing did not have an effect on the assay performance. This study demonstrates the feasibility of upconverting single-crystal nanoparticles for imaging-based detection of quantitative multianalyte assays. ..
  24. Lindström M, Jankola H, Hielm S, Hyytiä E, Korkeala H. Identification of Clostridium botulinum with API 20 A, Rapid ID 32 A and RapID ANA II. FEMS Immunol Med Microbiol. 1999;24:267-74 pubmed
    ..botulinum group II by the Rapid ID 32 A, 17 strains by the RapID ANA II, and one strain by the API 20 A. The results show that these test systems do not provide a reliable method for identification of C. botulinum. ..
  25. Saari J, Kivela T, Summanen P, Nummelin K, Saari K. Digital imaging in differential diagnosis of small choroidal melanoma. Graefes Arch Clin Exp Ophthalmol. 2006;244:1581-90 pubmed
    ..The subtraction method may reveal early growth of the melanotic choroidal tumors. ..
  26. Sillanpää H, Lahdenne P, Sarvas H, Arnez M, Steere A, Peltomaa M, et al. Immune responses to borrelial VlsE IR6 peptide variants. Int J Med Microbiol. 2007;297:45-52 pubmed
    ..In some manifestations of the disease, C6 ELISA may not cover all LB cases. Evidently, the methodological aspects in ELISA design for peptide antibody measurements are important as well as the amino acids sequence of the antigen. ..
  27. Vehtari A, Mäkinen V, Soininen P, Ingman P, Mäkelä S, Savolainen M, et al. A novel Bayesian approach to quantify clinical variables and to determine their spectroscopic counterparts in 1H NMR metabonomic data. BMC Bioinformatics. 2007;8 Suppl 2:S8 pubmed
    ..Nevertheless, the combination of high-quality quantification and the biochemical rationale of the resulting models is expected to be useful in the field of metabonomics. ..
  28. Manninen O, Koskenkorva P, Lehtimäki K, Hyppönen J, Kononen M, Laitinen T, et al. White matter degeneration with Unverricht-Lundborg progressive myoclonus epilepsy: a translational diffusion-tensor imaging study in patients and cystatin B-deficient mice. Radiology. 2013;269:232-9 pubmed publisher
    ..These data suggest that motor disturbances and other symptoms in patients with EPM1 involve not only the cortical system but also the thalamocortical system and cerebellum. ..
  29. Kotilainen P, Jalava J, Meurman O, Lehtonen O, Rintala E, Seppälä O, et al. Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid. J Clin Microbiol. 1998;36:2205-9 pubmed
    ..In a clinical setting, Gram staining and bacterial culture still remain the cornerstones of diagnosis. ..
  30. Kokkonen J, Leinonen A, Tuominen J, Seppala T. Comparison of sensitivity between gas chromatography-low-resolution mass spectrometry and gas chromatography-high-resolution mass spectrometry for determining metandienone metabolites in urine. J Chromatogr B Biomed Sci Appl. 1999;734:179-89 pubmed
    ..The results suggest that metandienone misuse can be detected by HRMS for a prolonged period after stopping the intake of metandienone. ..
  31. Rajaratnam R, Gylling H, Miettinen T. Independent association of serum squalene and noncholesterol sterols with coronary artery disease in postmenopausal women. J Am Coll Cardiol. 2000;35:1185-91 pubmed
    ..Thus, enhanced absorption and reduced synthesis of cholesterol may be related to coronary atherosclerosis. ..
  32. Gunell M, Antikainen P, Porjo N, Irjala K, Vakkila J, Hotakainen K, et al. Comprehensive real-time epidemiological data from respiratory infections in Finland between 2010 and 2014 obtained from an automated and multianalyte mariPOC® respiratory pathogen test. Eur J Clin Microbiol Infect Dis. 2016;35:405-13 pubmed publisher
    ..The results are reliable and give the clinician a picture of the current epidemiological situation, thus minimising guesswork. ..
  33. Varonen H, Savolainen S, Kunnamo I, Heikkinen R, Revonta M. Acute rhinosinusitis in primary care: a comparison of symptoms, signs, ultrasound, and radiography. Rhinology. 2003;41:37-43 pubmed
    ..If the criterion for AMS diagnosis were fluid in maxillary sinuses in ultrasound instead of clinical impression, the number of antibiotic prescriptions would be reduced by half in primary care. ..
  34. Ankelo M, Westerlund Karlsson A, Ilonen J, Knip M, Savola K, Kankaanpää P, et al. Time-resolved fluorometric assay for detection of autoantibodies to glutamic acid decarboxylase (GAD65). Clin Chem. 2003;49:908-15 pubmed
    ..It has a broad linear analytical range, is easy to perform and automate, and has sensitivity and specificity comparable to those for the conventional radioisotope assay. ..
  35. Nissila I, Hebden J, Jennions D, Heino J, Schweiger M, Kotilahti K, et al. Comparison between a time-domain and a frequency-domain system for optical tomography. J Biomed Opt. 2006;11:064015 pubmed
    ..The image quality of the reconstructions from the data measured with the two systems were compared with several quantitative criteria. In general a higher contrast was observed in the images calculated from the HUT data. ..
  36. Kaijalainen T, Rintamäki S, Herva E, Leinonen M. Evaluation of gene-technological and conventional methods in the identification of Streptococcus pneumoniae. J Microbiol Methods. 2002;51:111-8 pubmed
    ..Thus, they did not reliably help in the identification of suspected pneumococcal isolates lacking the conventional characteristics of pneumococcus. ..
  37. Karjalainen J, Tikkanen H, Hernelahti M, Kujala U. Muscle fiber-type distribution predicts weight gain and unfavorable left ventricular geometry: a 19 year follow-up study. BMC Cardiovasc Disord. 2006;6:2 pubmed
    ..Low type-I% predicts obesity and weight gain especially in the mid-abdomen, and consequently unfavourable LV geometry indicating increased cardiovascular risk. ..
  38. Scheinin A, Pienihakkinen K, Tiekso J, Holmberg S, Fukuda M, Suzuki A. Multifactorial modeling for root caries prediction: 3-year follow-up results. Community Dent Oral Epidemiol. 1994;22:126-9 pubmed
    ..8), Lactobacilli (OR 8.6) and Candida (OR 2.8). At screening, the criterion "two or three positive tests" of these yielded acceptable accuracy (77.1) and a relative risk of 3.3. ..
  39. Peltomaa M, Pyykko I, Seppala I, Viljanen M. Lyme borreliosis--an unusual cause of vertigo. Auris Nasus Larynx. 1998;25:233-42 pubmed
    ..Although Lyme borreliosis seems to be a rare cause of vertigo, it must be kept in mind in the differential diagnosis of vertigo...
  40. Piepponen T, Skujins A. Rapid and sensitive step gradient assays of glutamate, glycine, taurine and gamma-aminobutyric acid by high-performance liquid chromatography-fluorescence detection with o-phthalaldehyde-mercaptoethanol derivatization with an emphasis on microdialysi. J Chromatogr B Biomed Sci Appl. 2001;757:277-83 pubmed
    ..Total analysis time was less than 12 min, including the wash-out step. The methods described are relatively simple, sensitive, inexpensive, and fast enough to keep up with the microdialysis sampling...
  41. Qin Q, Laitinen P, Majamaa Voltti K, Eriksson S, Kumpula E, Pettersson K. Release patterns of pregnancy associated plasma protein A (PAPP-A) in patients with acute coronary syndromes. Scand Cardiovasc J. 2002;36:358-61 pubmed
    ..The possible clinical utility of PAPP-A calls for extensive studies of chest pain patients using serial sampling combined with short- and long-term outcome studies. ..
  42. von Lode P, Rosenberg J, Pettersson K, Takalo H. A europium chelate for quantitative point-of-care immunoassays using direct surface measurement. Anal Chem. 2003;75:3193-201 pubmed
    ..The novel nonadentate label facilitates short turnaround times and simple instrumentation due to the absence of all signal development steps, at the same time retaining an excellent immunoassay performance...
  43. Tuomisto T, Rissanen T, Vajanto I, Korkeela A, Rutanen J, Yla Herttuala S. HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. Atherosclerosis. 2004;174:111-20 pubmed
    ..These findings may be of importance in the design of novel therapies, such as therapeutic vascular growth, for patients suffering from lower limb ischemia. ..
  44. Rasinpera H, Savilahti E, Enattah N, Kuokkanen M, Tötterman N, Lindahl H, et al. A genetic test which can be used to diagnose adult-type hypolactasia in children. Gut. 2004;53:1571-6 pubmed
    ..03). Genetic test of C/T(-13910) polymorphism can be used as a first stage screening test for adult-type hypolactasia. ..
  45. Huhtinen P, Soukka T, Lövgren T, Harma H. Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology. J Immunol Methods. 2004;294:111-22 pubmed
    ..The general characteristics of this combination should permit the development of various immunoassay applications featuring high sensitivity, rapidity, and low consumption of reagents...
  46. Brader G, Sjöblom S, Hyytiäinen H, Sims Huopaniemi K, Palva E. Altering substrate chain length specificity of an acylhomoserine lactone synthase in bacterial communication. J Biol Chem. 2005;280:10403-9 pubmed
    ..Our data identify critical residues that define the size of the substrate-binding pocket of the AHSL synthase and will help in understanding and manipulating this bacterial language...
  47. Haaparanta M, Grönroos T, Eskola O, Bergman J, Solin O. Planar chromatographic analysis and quantification of short-lived radioactive metabolites from microdialysis fractions. J Chromatogr A. 2006;1108:136-9 pubmed
    ..The method was found to be highly sensitive and linear in the range of 0.1 Bq-2 kBq. This method thus allows the quantification of beta-emitting radiopharmaceuticals in sequential microdialysis fractions with good time-resolution...
  48. Hakanen A, Sipilä P, Sahla T. Characteristics of a 85Kr beta-particle source applied in Series 1 reference irradiations of DIS-1 direct ion storage dosemeters. Radiat Prot Dosimetry. 2007;124:103-6 pubmed
    ..The averaged zero angle H(p)(0.07) responses to the 90Sr + 90Y and 85Kr reference radiations were 135 and 80%, respectively. The responses were normalized to 100%, H(p)(0.07) response to 137Cs photon radiation...
  49. Valanne A, Suojanen J, Peltonen J, Soukka T, Hanninen P, Harma H. Multiple sized europium(III) chelate-dyed polystyrene particles as donors in FRET - an application for sensitive protein quantification utilizing competitive adsorption. Analyst. 2009;134:980-6 pubmed publisher
  50. Leinonen M, Nieminen P, Kotaniemi Talonen L, Malila N, Tarkkanen J, Laurila P, et al. Age-specific evaluation of primary human papillomavirus screening vs conventional cytology in a randomized setting. J Natl Cancer Inst. 2009;101:1612-23 pubmed publisher
    ..Among women aged 35 years or older, primary HPV DNA screening with cytology triage is also more specific than conventional screening and decreases colposcopy referrals and follow-up tests. ..
  51. Suominen P, Korpela R, Silfvast T, Olkkola K. Does water temperature affect outcome of nearly drowned children. Resuscitation. 1997;35:111-5 pubmed
    ..6% and specificity of 89.5% in predicting the outcome. In conclusion the effect of a potentially beneficial rapid development of hypothermia by cold water on the outcome of nearly drowned children could not be proved...
  52. Hujakka H, Koistinen V, Eerikäinen P, Kuronen I, Mononen I, Parviainen M, et al. New immunochromatographic rapid test for diagnosis of acute Puumala virus infection. J Clin Microbiol. 2001;39:2146-50 pubmed
  53. Hujakka H, Koistinen V, Eerikäinen P, Kuronen I, Laatikainen A, Kauppinen J, et al. Comparison of a new immunochromatographic rapid test with a commercial EIA for the detection of Puumala virus specific IgM antibodies. J Clin Virol. 2001;23:79-85 pubmed
    ..In addition, the rapid and straightforward procedure makes the POC PUUMALA a feasible tool for the diagnosis of the acute PUUV infection. ..
  54. Lindstrom M, Keto R, Markkula A, Nevas M, Hielm S, Korkeala H. Multiplex PCR assay for detection and identification of Clostridium botulinum types A, B, E, and F in food and fecal material. Appl Environ Microbiol. 2001;67:5694-9 pubmed
    ..botulinum type A, two contained type B, and one contained type E. The assay is sensitive and specific and provides a marked improvement in the PCR diagnostics of C. botulinum. ..
  55. Hirvonen Santti S, Rannikko A, Santti H, Savolainen S, Nyberg M, Janne O, et al. Down-regulation of estrogen receptor beta and transcriptional coregulator SNURF/RNF4 in testicular germ cell cancer. Eur Urol. 2003;44:742-7; discussion 747 pubmed
    ..Expression of ERbeta and SNURF/RNF4 was significantly lower in cancerous than in noncancerous testis tissue. Down-regulation of the ERbeta and the coregulator SNURF/RNF4 genes may play a role in testicular tumorigenesis. ..
  56. Stenman U, Alfthan H, Hotakainen K. Human chorionic gonadotropin in cancer. Clin Biochem. 2004;37:549-61 pubmed
    ..However, the reference range for hCG is 5-8 fold higher than that for hCGbeta and thus moderately elevated levels can be identified only with a specific and sensitive hCGbeta assay...
  57. Kopylov P, Fränti P. Compression of map images by multilayer context tree modeling. IEEE Trans Image Process. 2005;14:1-11 pubmed
    ..The proposed method gives results 50% better than JBIG and 25% better than a single-layer context tree modeling. ..
  58. Leskelä T, Tilsala Timisjärvi A, Kusnetsov J, Neubauer P, Breitenstein A. Sensitive genus-specific detection of Legionella by a 16S rRNA based sandwich hybridization assay. J Microbiol Methods. 2005;62:167-79 pubmed
    ..The results show that the developed SHA can be applied for estimation of the approximate number of Legionella cells based on the number of 16S rRNA molecules in a water sample...
  59. Jaakohuhta S, Harma H, Tuomola M, Lövgren T. Sensitive Listeria spp. immunoassay based on europium(III) nanoparticulate labels using time-resolved fluorescence. Int J Food Microbiol. 2007;114:288-94 pubmed
    ..The developed sensitive assay enabled shorter sample enrichment times and, therefore, faster analysis of Listeria spp. Obviously the detection of several other bacteria can also be enhanced by applying the nanoparticle assay technology...
  60. Pól J, Kauppila T, Haapala M, Saarela V, Franssila S, Ketola R, et al. Microchip sonic spray ionization. Anal Chem. 2007;79:3519-23 pubmed
    ..The microchip SSI provided dynamic linearity following a pattern similar to that used with electrospray, good quantitative repeatability (RSD=16%), and long-term signal stability. ..
  61. Alhonnoro T, Pollari M, Lilja M, Flanagan R, Kainz B, Muehl J, et al. Vessel segmentation for ablation treatment planning and simulation. Med Image Comput Comput Assist Interv. 2010;13:45-52 pubmed
    ..The results showed improved accuracy over common approaches and illustrated the method's suitability for simulation purposes. ..
  62. Shingfield K, Kairenius P, Arölä A, Paillard D, Muetzel S, Ahvenjärvi S, et al. Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal Butyrivibrio population in lactating cows. J Nutr. 2012;142:1437-48 pubmed publisher
    ..Dose-dependent increases in ruminal outflow of biohydrogenation intermediates containing one or more trans double bonds in response to FO has major implications for host metabolism and the nutritional quality of ruminant foods...
  63. Avoranta S, Korkeila E, Syrjanen K, Pyrhönen S, Sundström J. Lack of CD44 variant 6 expression in rectal cancer invasive front associates with early recurrence. World J Gastroenterol. 2012;18:4549-56 pubmed publisher
    ..022) when compared to the "front-positive" tumors. The lack of membranous CD44v6 in the rectal cancer invasive front could be used as a method to identify patients at increased risk for recurrent disease. ..
  64. Manninen H, Remes J, Partanen K, Tynkkynen P, Mykkanen L, Laakso M, et al. Evaluation of heart size and pulmonary vasculature. Conventional chest roentgenography and image intensifier photofluorography compared. Acta Radiol. 1991;32:226-31 pubmed
    ..18-0.59) while the intraobserver consistency (kappa coefficients 0.60-0.85) was good to excellent. The results suggest a limited usefulness of visual assessment of heart size and pulmonary vasculature in chest roentgenographs. ..
  65. Pasanen P, Partanen K, Pikkarainen P, Alhava E, Janatuinen E, Pirinen A. A comparison of ultrasound, computed tomography and endoscopic retrograde cholangiopancreatography in the differential diagnosis of benign and malignant jaundice and cholestasis. Eur J Surg. 1993;159:23-9 pubmed
    ..To assess the accuracy of ultrasonography (US), computed tomography (CT), and endoscopic retrograde cholangiopancreatography (ERCP) in distinguishing between benign and malignant causes of jaundice and cholestasis without jaundice...
  66. Hovi I, Taavitsainen M, Lantto T, Vorne M, Paul R, Remes K. Technetium-99m-HMPAO-labeled leukocytes and technetium-99m-labeled human polyclonal immunoglobulin G in diagnosis of focal purulent disease. J Nucl Med. 1993;34:1428-34 pubmed
    ..001). Thus, if focal infection is suspected, scintigraphy with 99mTc-labeled leukocytes is the preferable method. No cases exhibited a better imaging result with 99mTc-HIG scintigraphy than with 99mTc-labeled leukocytes...
  67. Koskinen L. Specificity of hemoglobin A1c measurement by cation exchange liquid chromatography. Evaluation of a Mono S column method. Clin Chim Acta. 1996;253:159-69 pubmed
    ..Three of these minor peaks were non-dependent on glucose. The chromatography and the peak integration method described can also be used to study hemoglobin adducts other than glycohemoglobin...
  68. Paavonen J. Pelvic inflammatory disease. From diagnosis to prevention. Dermatol Clin. 1998;16:747-56, xii pubmed
    ..Emerging evidence from randomized controlled trials provides strong evidence that intervention with selective screening for chlamydial infection effectively reduces the incidence of PID. ..
  69. Satokari R, Juvonen R, Mallison K, von Wright A, Haikara A. Detection of beer spoilage bacteria Megasphaera and Pectinatus by polymerase chain reaction and colorimetric microplate hybridization. Int J Food Microbiol. 1998;45:119-27 pubmed
  70. Haarala M, Kiiholma P, Nurmi M, Uksila J, Alanen A. The role of Borrelia burgdorferi in interstitial cystitis. Eur Urol. 2000;37:395-9 pubmed
    ..These results indicate that persistent infection of B. burgdorferi has no role in the etiology of IC. On the other hand a connection with a past borrelia infection and IC is not excluded. ..
  71. Hukkanen V, Rehn T, Kajander R, Sjöroos M, Waris M. Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid. J Clin Microbiol. 2000;38:3214-8 pubmed
    ..The TRF PCR assay for HSV in CSF specimens is a rapid and sensitive method, improves interpretation of PCR results, and is well suited for automation...
  72. Jussila M, Sinervo K, Porras S, Riekkola M. Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry. Electrophoresis. 2000;21:3311-7 pubmed
    ..The CE current was found to be stable and the migration times were highly reproducible. All the CE separations were carried out in a nonaqueous background electrolyte solution. ..
  73. Koivunen J, Lanki E, Rajala R, Siitonen A, Heinonen Tanski H. Determination of salmonellae from municipal wastewaters. Water Sci Technol. 2001;43:221-4 pubmed
    ..XLD and BM agars gave the highest numbers of salmonellae isolations but XLD and Rambach agars gave the best differentiation. Salmonella levels were < 3- > 1100 MPN/100 mL...
  74. Forsstrom T, Tuominen J, Kärkkäinen J. Determination of potentially hallucinogenic N-dimethylated indoleamines in human urine by HPLC/ESI-MS-MS. Scand J Clin Lab Invest. 2001;61:547-56 pubmed
    ..Method, procedure, considerations, statistical evaluations and urine sample spectra are presented...
  75. Svedström U, Vuorela H, Kostiainen R, Huovinen K, Laakso I, Hiltunen R. High-performance liquid chromatographic determination of oligomeric procyanidins from dimers up to the hexamer in hawthorn. J Chromatogr A. 2002;968:53-60 pubmed
    ..The correlation coefficients of calibration curves were between 0.997 and 1.000. The limits of quantitation for different procyanidin standards were 0.05-0.12 mg/ml, when 10 microl of each standard solution was injected into the HPLC...
  76. Madetoja J, Wiklund T. Detection of the fish pathogen Flavobacterium psychrophilum in water from fish farms. Syst Appl Microbiol. 2002;25:259-66 pubmed
    ..psychrophilum from environmental samples. It is suggested that IFAT and nested PCR provide effective tools for the examination of F. psychrophilum in the environment. ..
  77. Kalueff A, Tuohimaa P. The grooming analysis algorithm discriminates between different levels of anxiety in rats: potential utility for neurobehavioural stress research. J Neurosci Methods. 2005;143:169-77 pubmed
    ..Our results suggest that this method can be a useful tool in neurobehavioural stress research including modelling stress-evoked states, psychopharmacological or behavioural neurogenetics research in rats. ..
  78. Ahvenjarvi L, Koivukangas V, Jartti A, Ohtonen P, Saarnio J, Syrjala H, et al. Diagnostic accuracy of computed tomography imaging of surgically treated acute acalculous cholecystitis in critically ill patients. J Trauma. 2011;70:183-8 pubmed publisher
    ..Acute acalculous cholecystitis (AAC) is a potentially fatal condition mainly affecting critically ill patients. Current experience from computed tomography (CT) findings in AAC is contradictory...
  79. Hendolin P, Markkanen A, Ylikoski J, Wahlfors J. Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions. J Clin Microbiol. 1997;35:2854-8 pubmed
    ..Thus, the multiplex PCR method improves the detection rate significantly compared to that of the conventional culture method. ..
  80. Sudah M, Vanninen R, Partanen K, Heino A, Vainio P, Ala Opas M. MR urography in evaluation of acute flank pain: T2-weighted sequences and gadolinium-enhanced three-dimensional FLASH compared with urography. Fast low-angle shot. AJR Am J Roentgenol. 2001;176:105-12 pubmed
    ..The aim of this study was to compare the usefulness of breath-hold heavily T2-weighted sequences with gadolinium-enhanced three-dimensional fast low-angle shot (3D FLASH) MR urography in the evaluation of patients with acute flank pain...
  81. Svedström Oristo A, Pietila T, Pietilä I, Helenius H, Peutzfeldt P, Varrela J. Selection of criteria for assessment of occlusal acceptability. Acta Odontol Scand. 2002;60:160-6 pubmed
    ..Further studies are needed to analyse the reproducibility of assessment of the characteristics included...
  82. Kauppinen R, von und zu Fraunberg M. Molecular and biochemical studies of acute intermittent porphyria in 196 patients and their families. Clin Chem. 2002;48:1891-900 pubmed
    ..In addition, because biochemical analyses often remain indeterminate in remission, mutation analysis is needed to exclude or confirm the diagnosis of AIP. ..
  83. Soukka T, Antonen K, Harma H, Pelkkikangas A, Huhtinen P, Lövgren T. Highly sensitive immunoassay of free prostate-specific antigen in serum using europium(III) nanoparticle label technology. Clin Chim Acta. 2003;328:45-58 pubmed
    ..Our objective was to evaluate the performance of the nanoparticle label technology with biological samples in an immunoassay of free prostate-specific antigen (PSA-F) using a standard microtitration well platform...
  84. Sirola H, Kallio E, Koistinen V, Kuronen I, Lundkvist A, Vaheri A, et al. Rapid field test for detection of hantavirus antibodies in rodents. Epidemiol Infect. 2004;132:549-53 pubmed
    ..The test provides an applicable tool for studying PUUV and related hantavirus infections in arvicoline rodents. ..
  85. Koskinen W, Bono P, Leivo I, Vaheri A, Aaltonen L, Joensuu H. Lymphatic vessel density in vocal cord carcinomas assessed with LYVE-1 receptor expression. Radiother Oncol. 2005;77:172-5 pubmed
    ..2, 95% CI 1.1-4.5). Vocal cord carcinomas have a low lymph vessel density as compared with HNSCCs arising at other sites. ..
  86. Katzav M, Isohanni P, Lund M, Hakkinen M, Lyhs U. PCR assay for the detection of Campylobacter in marinated and non-marinated poultry products. Food Microbiol. 2008;25:908-14 pubmed publisher
    ..The PCR method used is faster than microbiological analyses. However, enrichment of the samples is necessary due to the low occurrence of Campylobacter spp. in retail Finnish poultry products. ..
  87. Sten T, Bichlmaier I, Kuuranne T, Leinonen A, Yli Kauhaluoma J, Finel M. UDP-glucuronosyltransferases (UGTs) 2B7 and UGT2B17 display converse specificity in testosterone and epitestosterone glucuronidation, whereas UGT2A1 conjugates both androgens similarly. Drug Metab Dispos. 2009;37:417-23 pubmed publisher
    ..The results shed new light on the substrate specificity and stereoselectivity of human UGTs. ..
  88. Rantakokko P, Kiviranta H, Rylander L, Rignell Hydbom A, Vartiainen T. A simple and fast liquid-liquid extraction method for the determination of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE) from human serum for epidemiological studies on type 2 diabetes. J Chromatogr A. 2009;1216:897-901 pubmed publisher
    ..The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden. ..
  89. Jääskeläinen A, Moilanen K, Bühler S, Lappalainen M, Vapalahti O, Vaheri A, et al. Serological microarray for detection of HSV-1, HSV-2, VZV, and CMV antibodies. J Virol Methods. 2009;160:167-71 pubmed publisher
    ..The serological microarray showed potential for screening purposes. The microarray based analyses were easy to perform, and HSV-1, HSV-2, VZV, and CMV antibodies could be detected on the same microarray...
  90. Jokela P, Piiparinen H, Mannonen L, Auvinen E, Lappalainen M. Performance of the Luminex xTAG Respiratory Viral Panel Fast in a clinical laboratory setting. J Virol Methods. 2012;182:82-6 pubmed publisher
    ..004) by RVP in comparison to real-time RT-PCR was observed. The results of the present study imply the need to adjust the InfA component of the RVP Fast assay to also cover the InfA(H1N1) 2009 virus...
  91. Ritvaniemi P, Hyland J, Ignatius J, Kivirikko K, Prockop D, Ala Kokko L. A fourth example suggests that premature termination codons in the COL2A1 gene are a common cause of the Stickler syndrome: analysis of the COL2A1 gene by denaturing gradient gel electrophoresis. Genomics. 1993;17:218-21 pubmed
  92. Hendolin P, Kärkkäinen U, Himi T, Markkanen A, Ylikoski J. High incidence of Alloiococcus otitis in otitis media with effusion. Pediatr Infect Dis J. 1999;18:860-5 pubmed
    ..The etiology of otitis media with effusion (OME) is unclear. Although the majority of effusions show inflammation, culture methods yield positive results for bacteria in only 20 to 30% of cases...