Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in United States
Locale: United States
Topic: saccharomyces cerevisiae proteins
Publications769 found, 100 shown here
- Singh K, Kang P, Park H. The Rho5 GTPase is necessary for oxidant-induced cell death in budding yeast. Proc Natl Acad Sci U S A. 2008;105:1522-7 pubmed publisher..Taken together, these data suggest that Rho5 mediates H(2)O(2)-induced cell death by regulating the level of Trr1 or by excluding Trr1 from its cytoplasmic substrate. ..
- Abdullah U, Cullen P. The tRNA modification complex elongator regulates the Cdc42-dependent mitogen-activated protein kinase pathway that controls filamentous growth in yeast. Eukaryot Cell. 2009;8:1362-72 pubmed publisher..Because protein translation provides a rough metric of cellular nutritional status, elongator may convey nutritional information to the filamentous growth pathway at the level of MSB2 expression. ..
- Lambrecht J, Browne B, Downs D. Members of the YjgF/YER057c/UK114 family of proteins inhibit phosphoribosylamine synthesis in vitro. J Biol Chem. 2010;285:34401-7 pubmed publisher..Thus, the work herein describes the first biochemical assay for diverse members of the highly conserved YjgF/YER057c/UK114 family of proteins and provides a means to dissect the cellular functions of these proteins. ..
- Paumi C, Pickin K, Jarrar R, Herren C, Cowley S. Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae. FEBS Lett. 2012;586:847-53 pubmed publisher..Our results suggest that during acute salt stress increased Sod1p, Sod2p and Ctt1p activity is the main compensatory for the loss in Ycf1p function that results from reduced Ycf1p-dependent recycling of cellular GSH levels...
- Hiraishi H, Shin B, Udagawa T, Nemoto N, Chowdhury W, Graham J, et al. Interaction between 25S rRNA A loop and eukaryotic translation initiation factor 5B promotes subunit joining and ensures stringent AUG selection. Mol Cell Biol. 2013;33:3540-8 pubmed publisher..This study provides an insight into the effect of ribosomal mutations on translation profiles in eukaryotes...
- Lemire S, Jeromin A, Boisselier Ã. Membrane binding of Neuronal Calcium Sensor-1 (NCS1). Colloids Surf B Biointerfaces. 2016;139:138-47 pubmed publisher..The myristoylation could thus have a structural role required in the folding/unfolding of NCS1 which is essential to its multiple biological functions. ..
- Muddukrishna B, Jackson C, Yu M. Protein arginine methylation of Npl3 promotes splicing of the SUS1 intron harboring non-consensus 5' splice site and branch site. Biochim Biophys Acta Gene Regul Mech. 2017;1860:730-739 pubmed publisher..Based on these data, we propose a model in which Hmt1, via arginine methylation of Npl3, facilitates U1 snRNP engagement with the pre-mRNA to promote usage of non-consensus splice sites by the splicing machinery. ..
- Conklin D, McMaster J, Culbertson M, Kung C. COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:3678-88 pubmed..The increased tolerance of strains containing multiple copies of the COT1 gene is probably due to increased compartmentalization or sequestration of the ion within mitochondria. ..
- Versaw W, Metzenberg R. Repressible cation-phosphate symporters in Neurospora crassa. Proc Natl Acad Sci U S A. 1995;92:3884-7 pubmed..The finding of such a system in a filamentous fungus is in harmony with the idea that the fungal and animal kingdoms are more closely related to each other than either is to the plant kingdom. ..
- Zhao H, Eide D. The ZRT2 gene encodes the low affinity zinc transporter in Saccharomyces cerevisiae. J Biol Chem. 1996;271:23203-10 pubmed..Finally, a zrt1zrt2 mutant was viable, indicating the existence of additional zinc uptake pathways. ..
- Paulovich A, Margulies R, Garvik B, Hartwell L. RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage. Genetics. 1997;145:45-62 pubmed
- Xu Z, Sato K, Wickner W. LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion. Cell. 1998;93:1125-34 pubmed..LMA1 is released from vacuoles in a phosphatase-regulated reaction. This LMA1 cycle explains how priming by Sec18p is coupled to t-SNARE stabilization and to fusion. ..
- Zheng P, Eastman J, Vande Pol S, Pimplikar S. PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein. Proc Natl Acad Sci U S A. 1998;95:14745-50 pubmed..Cotransfection of PAT1 with a reporter protein shows that PAT1 is functionally linked with intracellular transport of APP. We propose that PAT1 is involved in the translocation of APP along microtubules toward the cell surface. ..
- Culbertson M, Leeds P. Looking at mRNA decay pathways through the window of molecular evolution. Curr Opin Genet Dev. 2003;13:207-14 pubmed..Depending on the organism, three to seven genes are required for NMD. Besides RNA surveillance, the genes required for NMD serve a second purpose by controlling the overall abundance of a substantial fraction of the transcriptome...
- Salinas K, Wierzbicki S, Zhou L, Schmitt M. Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component. J Biol Chem. 2005;280:11352-60 pubmed..Based on this evidence, we propose that YLR145w encodes a novel protein component of RNase MRP, but not RNase P. We recommend that this gene be designated RMP1, for RNase MRP protein 1. ..
- Irvin J, Pugh B. Genome-wide transcriptional dependence on TAF1 functional domains. J Biol Chem. 2006;281:6404-12 pubmed
- Hirano Y, Fukunaga K, Sugimoto K. Rif1 and rif2 inhibit localization of tel1 to DNA ends. Mol Cell. 2009;33:312-22 pubmed publisher..Once Tel1 is delocalized, MRX does not associate efficiently with Rap1-covered DNA ends. These results reveal a mechanism by which telomeric DNA sequences mask DNA ends from Tel1 recognition for the regulation of telomere length. ..
- Wu C, Roje S, Sandoval F, Bird A, Winge D, Eide D. Repression of sulfate assimilation is an adaptive response of yeast to the oxidative stress of zinc deficiency. J Biol Chem. 2009;284:27544-56 pubmed publisher..These studies have led to new insights into how cells adapt to nutrient-limiting growth conditions. ..
- Pattenden S, Gogol M, Workman J. Features of cryptic promoters and their varied reliance on bromodomain-containing factors. PLoS ONE. 2010;5:e12927 pubmed publisher..Overall, our data indicate that cryptic promoters are independently regulated, and their activation is dependent on factors that govern gene activation at canonical promoters. ..
- Sasvari Z, Gonzalez P, Rachubinski R, Nagy P. Tombusvirus replication depends on Sec39p endoplasmic reticulum-associated transport protein. Virology. 2013;447:21-31 pubmed publisher..Overall, Sec39p is the first peroxisomal biogenesis protein characterized that is critical for tombusvirus replication in yeast and plants. ..
- Jung S, Smith J, von Haller P, Dilworth D, Sitko K, Miller L, et al. Global analysis of condition-specific subcellular protein distribution and abundance. Mol Cell Proteomics. 2013;12:1421-35 pubmed publisher..It also led to the identification of Fat3, a fatty acid transport protein of the plasma membrane, previously annotated as Ykl187. ..
- Manandhar S, Calle E, Gharakhanian E. Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae. J Biol Chem. 2014;289:11431-42 pubmed publisher..Thus, palmitoylation at the N-terminal cysteines of Env7 directs not only its membrane association but also its stability, phosphorylation, and cellular function. ..
- Yoon H, Miller S, Pabich E, Donahue T. SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast. Genes Dev. 1992;6:2463-77 pubmed..In light of the related effects of mutations in the SSL1 and SSL2 genes, the encoded proteins may functionally interact both to promote DNA repair and perform an essential function during translation initiation. ..
- Nagiec M, Wells G, Lester R, Dickson R. A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase. J Biol Chem. 1993;268:22156-63 pubmed
- Chen Y, McPhie D, Hirschberg J, Neve R. The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons. J Biol Chem. 2000;275:8929-35 pubmed..A specific caspase-6 inhibitor blocks this apoptosis. These findings are discussed in the context of abnormalities in the cell cycle that have been observed in Alzheimer's disease. ..
- Reynard G, Reynolds W, Verma R, Deshaies R. Cks1 is required for G(1) cyclin-cyclin-dependent kinase activity in budding yeast. Mol Cell Biol. 2000;20:5858-64 pubmed..The role of Cks1 in promoting G(1) cyclin-CDK protein kinase activity both in vitro and in vivo provides a simple molecular rationale for the essential role of CKS1 in progression through G(1) phase in budding yeast. ..
- Kelly T, Qin S, Gottschling D, Parthun M. Type B histone acetyltransferase Hat1p participates in telomeric silencing. Mol Cell Biol. 2000;20:7051-8 pubmed..Thus, in contrast to other histone acetyltransferases, Hat1p activity was required for transcriptional repression rather than gene activation. ..
- Hait N, Fujita K, Lester R, Dickson R. Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes. FEBS Lett. 2002;532:97-102 pubmed..These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded. ..
- Heidtman M, Chen C, Collins R, Barlowe C. A role for Yip1p in COPII vesicle biogenesis. J Cell Biol. 2003;163:57-69 pubmed..We propose that Yip1p has a previously unappreciated role in COPII vesicle biogenesis. ..
- West M, Hedges J, Lo K, Johnson A. Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. J Biol Chem. 2007;282:14028-37 pubmed..These results suggest that in the absence of the ribosome, Nmd3 is not efficiently released from Crm1 at the NPC. ..
- Schiller D. Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix. Int J Biochem Cell Biol. 2009;41:2343-9 pubmed publisher..Pam17 is involved in an early stage of protein translocation whereas Tim44 assists in a later step of transport, suggesting that both proteins can cooperate in a complementary manner in protein import. ..
- Martin Tumasz S, Butcher S. (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA. Biomol NMR Assign. 2009;3:227-30 pubmed publisher..In addition, nearly complete backbone assignments have been made for a Prp24 construct spanning the second and third RRMs (RRM23, residues M114-K290), both free and bound to AGAGAU. ..
- Viktorovskaya O, Appling F, Schneider D. Yeast transcription elongation factor Spt5 associates with RNA polymerase I and RNA polymerase II directly. J Biol Chem. 2011;286:18825-33 pubmed publisher..Based on these data, we propose a model in which Spt5 is recruited to the rDNA early in transcription and propose that it plays an important role in ribosomal RNA synthesis through direct binding to the Pol I complex. ..
- Ramachandran V, Shah K, Herman P. The cAMP-dependent protein kinase signaling pathway is a key regulator of P body foci formation. Mol Cell. 2011;43:973-81 pubmed publisher..This work therefore highlights the general relevance of RNP foci in quiescent cells, and provides a framework for the study of the many RNP assemblies that form in eukaryotic cells. ..
- Ma Z, Atencio D, Barnes C, Defiglio H, Hanes S. Multiple roles for the Ess1 prolyl isomerase in the RNA polymerase II transcription cycle. Mol Cell Biol. 2012;32:3594-607 pubmed publisher..Thus, Ess1 has direct effects on RNA polymerase transcription by controlling cofactor binding via conformationally induced changes in the CTD and indirect effects by influencing chromatin modification. ..
- Li X, Benning C, Kuo M. Rapid triacylglycerol turnover in Chlamydomonas reinhardtii requires a lipase with broad substrate specificity. Eukaryot Cell. 2012;11:1451-62 pubmed publisher..Together, these data suggest that CrLIP1 facilitates TAG turnover in Chlamydomonas primarily by degrading the DAG presumably generated from TAG hydrolysis. ..
- Zaborske J, Zeitler B, Culbertson M. Multiple transcripts from a 3'-UTR reporter vary in sensitivity to nonsense-mediated mRNA decay in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e80981 pubmed publisher..The results provide a proof of principle that long 3'-UTRs can trigger NMD, which suggests a potential link between errors in transcription termination or processing and mRNA decay. ..
- Wu X, Kim H, Seravalli J, Barycki J, Hart P, Gohara D, et al. Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. J Biol Chem. 2016;291:9796-806 pubmed publisher..Our study reveals a novel functional role of K(+) in the binding of copper to apoFet3p and identifies a K(+)/H(+) exchanger at the secretory pathway as a new molecular factor associated with iron uptake in yeast. ..
- Shaw L, Lewin A. The Cbp2 protein stimulates the splicing of the omega intron of yeast mitochondria. Nucleic Acids Res. 1997;25:1597-604 pubmed
- Park H, Sanson A, Herskowitz I. Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site. Genes Dev. 1999;13:1912-7 pubmed..These observations indicate that Bud2p becomes positioned in G(1) cells by recognizing cell type-specific landmarks at the presumptive bud site. ..
- Cipollo J, Trimble R. The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing. J Biol Chem. 2000;275:4267-77 pubmed
- Rush J, Cho S, Jiang S, Hofmann S, Waechter C. Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells. J Biol Chem. 2002;277:45226-34 pubmed
- Hearn J, Lester R, Dickson R. The uracil transporter Fur4p associates with lipid rafts. J Biol Chem. 2003;278:3679-86 pubmed..Further studies using lcb1-100 cells should reveal new roles of sphingolipids in nutrient uptake and other membrane-dependent processes. ..
- Shanower G, Muller M, Blanton J, Honti V, Gyurkovics H, Schedl P. Characterization of the grappa gene, the Drosophila histone H3 lysine 79 methyltransferase. Genetics. 2005;169:173-84 pubmed
- Sripathy S, Stevens J, Schultz D. The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression. Mol Cell Biol. 2006;26:8623-38 pubmed
- Narayanaswamy R, Moradi E, Niu W, Hart G, Davis M, McGary K, et al. Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast. J Proteome Res. 2009;8:6-19 pubmed publisher..The net effect is a defined ordering of major organelles along the polarization axis, with specific proteins implicated at the proximal growth tip. ..
- Singh I, Bhushan A, Relan N, Hashimoto T. Acyl-CoA ligases from rat brain microsomes: an immunochemical study. Biochim Biophys Acta. 1988;963:509-14 pubmed
- Halbrook J, Hoekstra M. Mutations in the Saccharomyces cerevisiae CDC1 gene affect double-strand-break-induced intrachromosomal recombination. Mol Cell Biol. 1994;14:8037-50 pubmed
- Ghosh B, Barbosa E, Singh I. Molecular cloning and sequencing of human palmitoyl-CoA ligase and its tissue specific expression. Mol Cell Biochem. 1995;151:77-81 pubmed..The expression of palmitoyl-CoA ligase in various tissue parallels the function of this enzyme in the metabolism of fatty acids in these tissues. ..
- Voisine C, Schilke B, Ohlson M, Beinert H, Marszalek J, Craig E. Role of the mitochondrial Hsp70s, Ssc1 and Ssq1, in the maturation of Yfh1. Mol Cell Biol. 2000;20:3677-84 pubmed
- Kastaniotis A, Mennella T, Konrad C, Torres A, Zitomer R. Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast. Mol Cell Biol. 2000;20:7088-98 pubmed
- Helfant A. Composition of the spindle pole body of Saccharomyces cerevisiae and the proteins involved in its duplication. Curr Genet. 2002;40:291-310 pubmed..In addition, genetic and biochemical studies have revealed the functional and physical relationships of certain spindle pole components and proteins important for SPB duplication. ..
- Li Q, Yang S, Maeda Y, Sladek F, Sharrocks A, Martins Green M. MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J. 2003;22:281-91 pubmed..The pre-assembly mechanism may greatly accelerate transcription activation, which is important in regulation of expression of immediate-early response genes, in particular those involved in stress responses. ..
- D Silva P, Schilke B, Walter W, Andrew A, Craig E. J protein cochaperone of the mitochondrial inner membrane required for protein import into the mitochondrial matrix. Proc Natl Acad Sci U S A. 2003;100:13839-44 pubmed..We propose that Pam18 is the J protein partner for Ssc1 at the import channel and is critical for Ssc1's function in protein import. ..
- Viswanathan P, Ohn T, Chiang Y, Chen J, Denis C. Mouse CAF1 can function as a processive deadenylase/3'-5'-exonuclease in vitro but in yeast the deadenylase function of CAF1 is not required for mRNA poly(A) removal. J Biol Chem. 2004;279:23988-95 pubmed..These results indicate that at least in yeast the CAF1 RNase activity is not required for its in vivo function. ..
- Xie Z, Liu S, Zhang Y, Wang Z. Roles of Rad23 protein in yeast nucleotide excision repair. Nucleic Acids Res. 2004;32:5981-90 pubmed
- West M, Hedges J, Chen A, Johnson A. Defining the order in which Nmd3p and Rpl10p load onto nascent 60S ribosomal subunits. Mol Cell Biol. 2005;25:3802-13 pubmed..These results support the idea that Sqt1p loads Rpl10p onto the Nmd3p-bound subunit after export to the cytoplasm and that Rpl10p loading involves the GTPase Lsg1p. ..
- Hung N, Johnson A. Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:3718-27 pubmed..Furthermore, these results suggest that Rei1 is needed for release of Arx1 from nascent 60S subunits after export to the cytoplasm but not for the subsequent nuclear import of Arx1. ..
- Ding M, Butler C, Saracco S, Fox T, Godard F, di Rago J, et al. Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins. Biochim Biophys Acta. 2008;1777:1147-56 pubmed publisher..This is the first time mutations have been created for three of the loci presented here, and nine of the resulting mutants have never been described before. ..
- Liu Y, Warfield L, Zhang C, Luo J, Allen J, Lang W, et al. Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol. 2009;29:4852-63 pubmed publisher..Genetic results suggest that Bur1 is essential for growth because it targets multiple factors that play distinct roles in transcription. ..
- Li Q, Fazly A, Zhou H, Huang S, Zhang Z, Stillman B. The elongator complex interacts with PCNA and modulates transcriptional silencing and sensitivity to DNA damage agents. PLoS Genet. 2009;5:e1000684 pubmed publisher..Together, these results uncover a novel role for the intact Elongator complex in transcriptional silencing and maintenance of genome stability, and it does so in a pathway linked to the DNA replication and DNA repair protein PCNA. ..
- Ohkuni K, Kitagawa K. Endogenous transcription at the centromere facilitates centromere activity in budding yeast. Curr Biol. 2011;21:1695-703 pubmed publisher..Dig1 represses Ste12-dependent transcription at the centromere. Our studies reveal that transcription at the centromere plays an important role in centromere function in budding yeast. ..
- Daithankar V, Wang W, Trujillo J, Thorpe C. Flavin-linked Erv-family sulfhydryl oxidases release superoxide anion during catalytic turnover. Biochemistry. 2012;51:265-72 pubmed publisher..Aspects of the mechanism and possible physiological significance of superoxide release from these Erv-domain flavoproteins are discussed. ..
- Torabi N, Kruglyak L. Genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast. PLoS Genet. 2012;8:e1002546 pubmed publisher..We found that the allelic state of SKY1 interacts with readthrough level and the genetic background to determine growth rate in these two conditions. ..
- Dewe J, Whipple J, Chernyakov I, Jaramillo L, Phizicky E. The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications. RNA. 2012;18:1886-96 pubmed publisher..These results demonstrate that RTD interacts with the translation machinery and acts widely on hypomodified tRNAs. ..
- Paeschke K, Bochman M, Garcia P, Cejka P, Friedman K, Kowalczykowski S, et al. Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature. 2013;497:458-62 pubmed publisher..Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening. ..
- Guo X, Niemi N, Coon J, Pagliarini D. Integrative proteomics and biochemical analyses define Ptc6p as the Saccharomyces cerevisiae pyruvate dehydrogenase phosphatase. J Biol Chem. 2017;292:11751-11759 pubmed publisher..Our findings highlight the power of integrative omics and biochemical analyses for annotating the functions of poorly characterized signaling proteins. ..
- Errabolu R, Sanders M, Salisbury J. Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles. J Cell Sci. 1994;107 ( Pt 1):9-16 pubmed
- Takahara K, Bong M, Brevard R, Eddy R, Haley L, Sait S, et al. Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L. Genomics. 1996;31:119-22 pubmed..Analyses of somatic cell hybrid DNA and fluorescence in situ hybridization were employed to map the human ORC2L gene to chromosome 2q33. ..
- Keith K, Fitzgerald Hayes M. CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome. Genetics. 2000;156:973-81 pubmed..On the basis of these and other results from genetic, biochemical, and structural studies, we propose a model that best describes the path of the centromere DNA around a specialized Cse4p-nucleosome. ..
- Shou W, Deshaies R. Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet. 2002;3:4 pubmed..Transport-defective mutations in two karyopherins can bypass cdc15Delta, suggesting that the function of the MEN is to promote mitotic exit by regulating nuclear transport. ..
- Zhou J, Qi H, Schulz V, Mateyak M, Monson E, Zakian V. Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases. Mol Biol Cell. 2002;13:2180-91 pubmed..Telomeric DNA was modestly shortened in the absence of Pfh1p. However, genetic analysis demonstrated that maintenance of telomeric DNA was not the sole essential function of S. pombe Pfh1p. ..
- van Leeuwen F, Gafken P, Gottschling D. Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell. 2002;109:745-56 pubmed..Because Dot1p and histone H3 are conserved, similar mechanisms are likely at work in other eukaryotes. ..
- Kominsky D, Brownson M, Updike D, Thorsness P. Genetic and biochemical basis for viability of yeast lacking mitochondrial genomes. Genetics. 2002;162:1595-604 pubmed
- Kou H, Irvin J, Huisinga K, Mitra M, Pugh B. Structural and functional analysis of mutations along the crystallographic dimer interface of the yeast TATA binding protein. Mol Cell Biol. 2003;23:3186-201 pubmed..These phenotypes cannot be accounted for by defective interactions with other known TBP inhibitors and likely reflect defects in TBP dimerization. ..
- Boulé J, Vega L, Zakian V. The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature. 2005;438:57-61 pubmed..We propose that Pif1p helicase activity limits telomerase action both in vivo and in vitro by displacing active telomerase from DNA ends. ..
- Fratti R, Collins K, Hickey C, Wickner W. Stringent 3Q.1R composition of the SNARE 0-layer can be bypassed for fusion by compensatory SNARE mutation or by lipid bilayer modification. J Biol Chem. 2007;282:14861-7 pubmed..Thus, SNARE function as regulated by the 0-layer is intimately coupled to the lipids, which must rearrange for fusion. ..
- Nagaraj S, Rajendran A, Jackson C, Longtine M. Role of nucleotide binding in septin-septin interactions and septin localization in Saccharomyces cerevisiae. Mol Cell Biol. 2008;28:5120-37 pubmed publisher..We find no evidence that nucleotide binding is specifically involved in the interaction of septins with septin-associated proteins. ..
- Li H, Gakh O, Smith D, Isaya G. Oligomeric yeast frataxin drives assembly of core machinery for mitochondrial iron-sulfur cluster synthesis. J Biol Chem. 2009;284:21971-80 pubmed publisher..These findings suggest that iron-dependent oligomerization is a mechanism by which the iron donor promotes assembly of the core machinery for mitochondrial ISC synthesis. ..
- Piccirillo S, White M, Murphy J, Law D, Honigberg S. The Rim101p/PacC pathway and alkaline pH regulate pattern formation in yeast colonies. Genetics. 2010;184:707-16 pubmed publisher..Thus, the Rim101 pathway mediates colony patterning by responding to cell-to-cell pH signals. Cell-to-cell signals coupled with nutrient gradients may allow efficient spore formation and spore dispersal in natural environments. ..
- Serang O, MacCoss M, Noble W. Efficient marginalization to compute protein posterior probabilities from shotgun mass spectrometry data. J Proteome Res. 2010;9:5346-57 pubmed publisher..We evaluate our identification procedure on five different well-characterized data sets and demonstrate our ability to efficiently compute high-quality protein posteriors. ..
- D Silva S, Haider S, Phizicky E. A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop. RNA. 2011;17:1100-10 pubmed publisher..We also suggest that m³C has a role in translation, since trm140-? trm1-? strains (also lacking m²,²G??) are sensitive to low concentrations of cycloheximide. ..
- Tsuchiya D, Gonzalez C, Lacefield S. The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:2848-61 pubmed publisher..We propose that Mad2 is an important meiotic cell cycle regulator that ensures the timely degradation of APC/C substrates and the proper orchestration of the meiotic divisions. ..
- Akiyoshi B, Nelson C, Duggan N, Ceto S, Ranish J, Biggins S. The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein. PLoS Genet. 2013;9:e1003216 pubmed publisher..We propose that Mub1/Ubr2 are part of a quality control system that monitors kinetochore integrity, thus ensuring genomic stability. ..
- Yonashiro R, Tahara E, Bengtson M, Khokhrina M, Lorenz H, Chen K, et al. The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation. elife. 2016;5:e11794 pubmed publisher..These findings uncover a translational stalling-dependent protein aggregation mechanism, and provide evidence that proteins can become specifically marked for aggregation. ..
- Azzam R, Chen S, Shou W, Mah A, Alexandru G, Nasmyth K, et al. Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. Science. 2004;305:516-9 pubmed..Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis. ..
- Rice T, Ding M, Pederson D, Heintz N. The highly conserved tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex and is required for the G2/M phase transition in the yeast Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:832-5 pubmed..Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast. ..
- Fukunaga K, Kwon Y, Sung P, Sugimoto K. Activation of protein kinase Tel1 through recognition of protein-bound DNA ends. Mol Cell Biol. 2011;31:1959-71 pubmed publisher..The mre11-3 mutation abolishes DNA end-processing activity but does not affect the ability to enhance Tel1 activation. These results support a model in which MRX controls Tel1 activation by recognizing protein-bound DNA ends. ..