Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Switzerland
Topic: saccharomyces cerevisiae proteins
Publications189 found, 100 shown here
- Wanke V, Cameroni E, Uotila A, Piccolis M, Urban J, Loewith R, et al. Caffeine extends yeast lifespan by targeting TORC1. Mol Microbiol. 2008;69:277-85 pubmed publisher..This kinase cascade appears to have been evolutionarily conserved, suggesting that caffeine may extend lifespan in other eukaryotes, including man. ..
- Kruschel D, Sigel R. Divalent metal ions promote the formation of the 5'-splice site recognition complex in a self-splicing group II intron. J Inorg Biochem. 2008;102:2147-54 pubmed publisher..These observations illustrate that a high charge density independent of the nature of the ion is needed for binding EBS1 to IBS1, but divalent metal ions are presumably the better players. ..
- Gander S, Martin D, Hauri S, Moes S, Poletto G, Pagano M, et al. A modified KESTREL search reveals a basophilic substrate consensus for the Saccharomyces cerevisiae Npr1 protein kinase. J Proteome Res. 2009;8:5305-16 pubmed publisher..The availability of a consensus sequence allows a targeted search for physiologically relevant Npr1 substrates involved in the regulation of yeast amino acid permeases. ..
- Pelet S, Rudolf F, Nadal Ribelles M, de Nadal E, Posas F, Peter M. Transient activation of the HOG MAPK pathway regulates bimodal gene expression. Science. 2011;332:732-5 pubmed publisher..Together, these findings provide a molecular mechanism by which a cell can impose a transcriptional threshold in response to a linear signaling behavior. ..
- Nikolaev Y, Pervushin K. Structural basis of RNA binding by leucine zipper GCN4. Protein Sci. 2012;21:667-76 pubmed publisher..Further studies in the context of full-length bZIP factors are envisaged to address the biological relevance of LZ RNase activity. ..
- Leighton J, Schatz G. An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast. EMBO J. 1995;14:188-95 pubmed..Atm1p is the first mitochondrial member of the ABC family to be identified and the only eukaryotic ABC transporter that has been shown to be necessary for normal cellular growth...
- Funato K, Riezman H. Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast. J Cell Biol. 2001;155:949-59 pubmed..A cytosolic protease-sensitive factor is required after establishment of ER-Golgi contacts. ..
- Shimada K, Pasero P, Gasser S. ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase. Genes Dev. 2002;16:3236-52 pubmed..Our data suggest the existence of an S-phase-specific threshold that may be necessary to allow cells to tolerate damage-like DNA structures present at normal replication forks. ..
- Taddei A, Gasser S. Multiple pathways for telomere tethering: functional implications of subnuclear position for heterochromatin formation. Biochim Biophys Acta. 2004;1677:120-8 pubmed..This approach, combined with genetics and functional assays, has shed new light on how nuclear compartments facilitate gene repression in yeast. ..
- Berset T, Hoier E, Hajnal A. The C. elegans homolog of the mammalian tumor suppressor Dep-1/Scc1 inhibits EGFR signaling to regulate binary cell fate decisions. Genes Dev. 2005;19:1328-40 pubmed..Mammalian Dep-1/Scc1 functions as a tumor-suppressor gene in the intestinal epithelium. Thus, mutations in human Dep-1 may promote tumor formation through a hyperactivation of the EGF receptor. ..
- Cottier V, Barberis A, Lüthi U. Novel yeast cell-based assay to screen for inhibitors of human cytomegalovirus protease in a high-throughput format. Antimicrob Agents Chemother. 2006;50:565-71 pubmed..The growth selection system presented here provides the basis for a high-throughput screen to identify HCMV protease inhibitors that are active in eukaryotic cells. ..
- Dubrana K, van Attikum H, Hediger F, Gasser S. The processing of double-strand breaks and binding of single-strand-binding proteins RPA and Rad51 modulate the formation of ATR-kinase foci in yeast. J Cell Sci. 2007;120:4209-20 pubmed..These observations led to a model that links end-processing and competition between different ssDNA-binding factors with Mec1-Ddc2 focus formation and checkpoint activation. ..
- Garas M, Dichtl B, Keller W. The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis. RNA. 2008;14:2671-84 pubmed publisher..These observations suggest that Ysh1p has multiple roles in RNA synthesis and processing. ..
- Lempiäinen H, Uotila A, Urban J, Dohnal I, Ammerer G, Loewith R, et al. Sfp1 interaction with TORC1 and Mrs6 reveals feedback regulation on TOR signaling. Mol Cell. 2009;33:704-16 pubmed publisher..Finally, we show that the Sfp1-interacting protein Mrs6, a Rab escort protein involved in membrane trafficking, regulates both Sfp1 nuclear localization and TORC1 signaling. ..
- Binda M, Péli Gulli M, Bonfils G, Panchaud N, Urban J, Sturgill T, et al. The Vam6 GEF controls TORC1 by activating the EGO complex. Mol Cell. 2009;35:563-73 pubmed publisher..Thus, in addition to its regulatory role in homotypic vacuolar fusion and vacuole protein sorting within the HOPS complex, Vam6 also controls TORC1 function by activating the Gtr1 subunit of the EGO complex. ..
- Kemmler S, Occhipinti L, Veisu M, Panse V. Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol. 2009;186:863-80 pubmed publisher..Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits. ..
- Preker P, Ohnacker M, Minvielle Sebastia L, Keller W. A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J. 1997;16:4727-37 pubmed..PF I also appears to be functionally related to CPSF, as it polyadenylates a substrate RNA more efficiently than Pap1p alone. Possibly, the observed interaction of the complex with RNA tethers Pap1p to its substrate. ..
- Wesp A, Hicke L, Palecek J, Lombardi R, Aust T, Munn A, et al. End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:2291-306 pubmed..Combined biochemical, functional, and genetic analysis lead us to propose that End4p may mediate endocytosis through interaction with other actin-associated proteins, perhaps Rvs167p, a protein essential for endocytosis. ..
- Galan J, Peter M. Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism. Proc Natl Acad Sci U S A. 1999;96:9124-9 pubmed
- Lafourcade C, Galan J, Peter M. Opposite roles of the F-box protein Rcy1p and the GTPase-activating protein Gyp2p during recycling of internalized proteins in yeast. Genetics. 2003;164:469-77 pubmed..Taken together, our results suggest that the F-box protein Rcy1p may activate the Ypt6p GTPase module during recycling. ..
- Zechner C, Ruess J, Krenn P, Pelet S, Peter M, Lygeros J, et al. Moment-based inference predicts bimodality in transient gene expression. Proc Natl Acad Sci U S A. 2012;109:8340-5 pubmed publisher..Our results show that measurements of the mean and the variance can be enough to determine the model parameters, even if the measured distributions are not well-characterized by low-order moments only--e.g., if they are bimodal. ..
- SchÃ¼tz M, SchÃ¶ppe J, SedlÃ¡k E, Hillenbrand M, Nagy Davidescu G, Ehrenmann J, et al. Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts. Sci Rep. 2016;6:21508 pubmed publisher..Thus, the presented method broadens the portfolio of GPCRs accessible for detailed investigations. Interestingly, the functional production of GPCRs in yeast can be further increased by induced host adaptation. ..
- Schneider A, Oppliger W, Jeno P. Purified inner membrane protease I of yeast mitochondria is a heterodimer. J Biol Chem. 1994;269:8635-8 pubmed..Partial amino acid sequencing identifies this subunit as the product of the recently described IMP2 gene (Nunnari, J., Fox, T., and Walter, P. (1993) Science 262, 1997-2004). ..
- Hines V, Schatz G. Precursor binding to yeast mitochondria. A general role for the outer membrane protein Mas70p. J Biol Chem. 1993;268:449-54 pubmed..We conclude that Mas70p is an import receptor for most, if not all authentic mitochondrial precursor proteins, but that its function is not always rate-limiting in import experiments with isolated mitochondria. ..
- De Rubertis F, Kadosh D, Henchoz S, Pauli D, Reuter G, Struhl K, et al. The histone deacetylase RPD3 counteracts genomic silencing in Drosophila and yeast. Nature. 1996;384:589-91 pubmed..This function of RPD3, which is in contrast to the general correlation between histone acetylation and increased transcription, might be due to a specialized chromatin structure at silenced loci. ..
- Schmidt A, Bickle M, Beck T, Hall M. The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Cell. 1997;88:531-42 pubmed..Thus, TOR2 signals to the actin cytoskeleton through a GTPase switch composed of RHO1, RHO2, ROM2, and SAC7. TOR2 activates this switch via ROM2, possibly via the ROM2 PH domain. ..
- Reymond F, Wirbelauer C, Krek W. Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase. J Cell Sci. 2000;113 ( Pt 10):1687-94 pubmed
- Flury I, Benachour A, Conzelmann A. YLL031c belongs to a novel family of membrane proteins involved in the transfer of ethanolaminephosphate onto the core structure of glycosylphosphatidylinositol anchors in yeast. J Biol Chem. 2000;275:24458-65 pubmed..e. the group that links the GPI lipid anchor to proteins, whereas Mcd4p and Gpi7p transfer ethanolaminephosphate onto the alpha1-4- and alpha1-6-linked mannoses of the GPI anchor, respectively. ..
- Schmelzle T, Hall M. TOR, a central controller of cell growth. Cell. 2000;103:253-62 pubmed
- Crespo J, Daicho K, Ushimaru T, Hall M. The GATA transcription factors GLN3 and GAT1 link TOR to salt stress in Saccharomyces cerevisiae. J Biol Chem. 2001;276:34441-4 pubmed..In summary, our results suggest that TOR plays a role in the general response to saline stress by regulating the transcription of ENA1 via GLN3 and GAT1. ..
- Hoepfner D, Van den Berg M, Philippsen P, Tabak H, Hettema E. A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. J Cell Biol. 2001;155:979-90 pubmed..In addition, time-lapse videos showing peroxisome movement in wild-type and vps1Delta cells suggest the existence of various levels of control involved in the partitioning of peroxisomes. ..
- Dichtl B, Blank D, Ohnacker M, Friedlein A, Roeder D, Langen H, et al. A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol Cell. 2002;10:1139-50 pubmed..The sum of our analyses suggests a negative influence of Ssu72p on RNAP II during transcription that affects the commitment to either elongation or termination. ..
- Schmelzle T, Beck T, Martin D, Hall M. Activation of the RAS/cyclic AMP pathway suppresses a TOR deficiency in yeast. Mol Cell Biol. 2004;24:338-51 pubmed..Our findings suggest that TOR signals through the RAS/cAMP pathway, independently of TAP42/SIT4. Therefore, the RAS/cAMP pathway may be a novel TOR effector branch. ..
- Gaigg B, Toulmay A, Schneiter R. Very long-chain fatty acid-containing lipids rather than sphingolipids per se are required for raft association and stable surface transport of newly synthesized plasma membrane ATPase in yeast. J Biol Chem. 2006;281:34135-45 pubmed..We thus conclude that the essential function of sphingolipids for membrane domain formation and stable surface delivery of Pma1p is provided by the C26 fatty acid that forms part of the yeast ceramide. ..
- Jaquenoud M, Pagac M, Signorell A, Benghezal M, Jelk J, Bütikofer P, et al. The Gup1 homologue of Trypanosoma brucei is a GPI glycosylphosphatidylinositol remodelase. Mol Microbiol. 2008;67:202-12 pubmed..Thus, tbGup1p is involved in the addition of myristate to sn-2 during GPI remodelling in T. brucei and can account for the fatty acid specificity of this process. tbGup1p can act on GPI proteins as well as on GPI lipids. ..
- Haruki H, Nishikawa J, Laemmli U. The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes. Mol Cell. 2008;31:925-32 pubmed publisher..The AA technique is a powerful tool for nuclear biology to dissect the function of individual or gene pairs in synthetic, lethal situations. ..
- Gander S, Bonenfant D, Altermatt P, Martin D, Hauri S, Moes S, et al. Identification of the rapamycin-sensitive phosphorylation sites within the Ser/Thr-rich domain of the yeast Npr1 protein kinase. Rapid Commun Mass Spectrom. 2008;22:3743-53 pubmed publisher..Site-directed mutagenesis confirmed the mass spectral assignments of the autophosphorylation sites and shows that Ser257 is specifically involved in forming an in vitro substrate-binding site. ..
- Kilchert C, Spang A. Cotranslational transport of ABP140 mRNA to the distal pole of S. cerevisiae. EMBO J. 2011;30:3567-80 pubmed publisher..We therefore propose a model of translational coupling, in which ABP140 mRNA is tethered to actin cables via its nascent protein product and is transported to the distal pole by actin retrograde flow. ..
- Picotti P, Clément Ziza M, Lam H, Campbell D, Schmidt A, Deutsch E, et al. A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis. Nature. 2013;494:266-70 pubmed publisher..Our results suggest that selective pressure favours the acquisition of sets of polymorphisms that adapt protein levels but also maintain the stoichiometry of functionally related pathway members. ..
- Talarek N, Bontron S, De Virgilio C. Quantification of mRNA stability of stress-responsive yeast genes following conditional excision of open reading frames. RNA Biol. 2013;10:1299-308 pubmed publisher..These data pinpoint PP2A(Cdc55) as a central node in homo-directional coordination of transcription and post-transcriptional mRNA stabilization of a specific array of nutrient-regulated genes. ..
- John Peter A, Herrmann B, Antunes D, Rapaport D, Dimmer K, Kornmann B. Vps13-Mcp1 interact at vacuole-mitochondria interfaces and bypass ER-mitochondria contact sites. J Cell Biol. 2017;216:3219-3229 pubmed publisher..Tethered and functionally active vacuole-mitochondria interfaces then compensate for the loss of ERMES-mediated ER-mitochondria contact sites. ..
- Coppolecchia R, Buser P, Stotz A, Linder P. A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase. EMBO J. 1993;12:4005-11 pubmed..The Stm1 protein has potential RNP1 and RNP2 motifs characteristic for RNA-binding proteins. The protein also contains six highly conserved direct repeats of 21-26 amino acids and one partial repeat. ..
- Munn A, Stevenson B, Geli M, Riezman H. end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae. Mol Biol Cell. 1995;6:1721-42 pubmed..We propose that Vrp1p, Rvs161p, and Rvs167p are components of a cytoskeletal structure that contains actin and fimbrin and that is required for formation of endocytic vesicles at the plasma membrane. ..
- Meyer U, Benghezal M, Imhof I, Conzelmann A. Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins. Biochemistry. 2000;39:3461-71 pubmed..The dominant negative effect of nonfunctional and partially functional mutant alleles can best be explained by assuming that Gpi8p works as part of a homo- or heteropolymeric complex. ..
- Wiederkehr A, Meier K, Riezman H. Identification and characterization of Saccharomyces cerevisiae mutants defective in fluid-phase endocytosis. Yeast. 2001;18:759-73 pubmed..Some of the mutants identified in this screen that sort carboxypeptidase Y correctly may affect endocytosis at an early post-internalization step before the intersection of the endocytic with the vacuolar protein-sorting pathway. ..
- Castillon G, Aguilera Romero A, Manzano López J, Epstein S, Kajiwara K, Funato K, et al. The yeast p24 complex regulates GPI-anchored protein transport and quality control by monitoring anchor remodeling. Mol Biol Cell. 2011;22:2924-36 pubmed publisher..Therefore the p24 complex, by sensing the status of the GPI anchor, regulates GPI-anchored protein intracellular transport and coordinates this with correct anchor remodeling. ..
- Spang A, Shiba Y, Randazzo P. Arf GAPs: gatekeepers of vesicle generation. FEBS Lett. 2010;584:2646-51 pubmed publisher..We discuss the roles of Arf GAPs as regulators and effectors for Arf GTP-binding proteins. ..
- Garcia Bustos J, Marini F, Stevenson I, Frei C, Hall M. PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus. EMBO J. 1994;13:2352-61 pubmed..Conditional mutants have a cytokinesis defect as indicated by a uniform terminal phenotype of cells with large buds and fully divided nuclei. We suggest that PIK1 controls cytokinesis through the actin cytoskeleton. ..
- Geli M, Wesp A, Riezman H. Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets. EMBO J. 1998;17:635-47 pubmed..Additionally, genetic and biochemical evidence obtained with a collection of cmd1 mutant alleles strongly suggests that Cmd1p plays an additional role in the internalization step of receptor-mediated endocytosis in yeast. ..
- Pedrazzi G, Perrera C, Blaser H, Kuster P, Marra G, Davies S, et al. Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1. Nucleic Acids Res. 2001;29:4378-86 pubmed..We conclude that the hMLH1-BLM interaction is not essential for post-replicative MMR, but, more likely, is required for some aspect of genetic recombination. ..
- Meier K, Deloche O, Kajiwara K, Funato K, Riezman H. Sphingoid base is required for translation initiation during heat stress in Saccharomyces cerevisiae. Mol Biol Cell. 2006;17:1164-75 pubmed..Taken together, we have shown a requirement for sphingoid bases during the recovery from heat shock and suggest that this reflects a direct lipid-dependent signal to the cap-dependent translation initiation apparatus. ..
- Schneider A. Mitochondrial tRNA import and its consequences for mitochondrial translation. Annu Rev Biochem. 2011;80:1033-53 pubmed publisher..For some tRNAs, this is not trivial and requires unique evolutionary adaptations. ..
- Hamburger D, Egerton M, Riezman H. Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins. J Cell Biol. 1995;129:629-39 pubmed..Therefore, Gaa1p is required for a terminal step of GPI anchor attachment and could be part of the putative GPI:protein transamidase. ..
- Alcedo J, Zou Y, Noll M. Posttranscriptional regulation of smoothened is part of a self-correcting mechanism in the Hedgehog signaling system. Mol Cell. 2000;6:457-65 pubmed
- Dudognon P, Maeder Garavaglia C, Carpentier J, Paccaud J. Regulation of a COPII component by cytosolic O-glycosylation during mitosis. FEBS Lett. 2004;561:44-50 pubmed..These data suggest that when entering into mitosis, the COPII component Sec24p is simultaneously deglycosylated and phosphorylated, a process which may contribute to the observed mitotic ER-to-Golgi traffic block. ..
- Szathmary R, Bielmann R, Nita Lazar M, Burda P, Jakob C. Yos9 protein is essential for degradation of misfolded glycoproteins and may function as lectin in ERAD. Mol Cell. 2005;19:765-75 pubmed..Yos9 protein is important for glycoprotein degradation and may act via its MRH domain as a degradation lectin-like protein in the glycoprotein degradation pathway. ..
- Schaerer Brodbeck C, Riezman H. Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons. J Cell Sci. 2000;113 ( Pt 3):521-32 pubmed..These data suggest that the actin defect is probably not the cause of the microtubule defect. ..
- Prescianotto Baschong C, Riezman H. Ordering of compartments in the yeast endocytic pathway. Traffic. 2002;3:37-49 pubmed..On the other hand, traffic to the early recycling endosome is less dependent on Rab5 homologs and does not require Vps27p. ..
- Dion V, Kalck V, Horigome C, Towbin B, Gasser S. Increased mobility of double-strand breaks requires Mec1, Rad9 and the homologous recombination machinery. Nat Cell Biol. 2012;14:502-9 pubmed publisher..Consistent with a role for movement in the homology-search step of homologous recombination, we show that recombination intermediates take longer to form in cells lacking Rad9. ..
- Grüter P, Tabernero C, von Kobbe C, Schmitt C, Saavedra C, Bachi A, et al. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol Cell. 1998;1:649-59 pubmed..Thus, TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator. TAP is the second cellular RNA binding protein shown to be directly involved in the export of its target RNA...
- Fahrenkrog B, Hurt E, Aebi U, Pante N. Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol. 1998;143:577-88 pubmed..Accordingly, Nsp1p resides in three distinct subcomplexes which are located at the entry and exit of the central gated channel and at the terminal ring of the nuclear basket. ..
- Sadowski M, Dichtl B, Hubner W, Keller W. Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination. EMBO J. 2003;22:2167-77 pubmed..We conclude that Pcf11p is a bifunctional protein and that transcript cleavage is not an obligatory step prior to RNAP II termination. ..
- Breuza L, Halbeisen R, Jeno P, Otte S, Barlowe C, Hong W, et al. Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. J Biol Chem. 2004;279:47242-53 pubmed..Our novel approach for the isolation of the ERGIC from BFA-treated cells may ultimately lead to the identification of all proteins rapidly cycling early in the secretory pathway. ..
- Haucke V, Horst M, Schatz G, Lithgow T. The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J. 1996;15:1231-7 pubmed..We conclude that the two receptor subcomplexes of the mitochondrial protein import receptor interact in vivo via their Mas20p and Mas70p subunits and that this interaction is functionally important. ..
- Niederberger N, Trachsel H, Altmann M. The RNA recognition motif of yeast translation initiation factor Tif3/eIF4B is required but not sufficient for RNA strand-exchange and translational activity. RNA. 1998;4:1259-67 pubmed
- Badi L, Barberis A. Proteins that genetically interact with the Saccharomyces cerevisiae transcription factor Gal11p emphasize its role in the initiation-elongation transition. Mol Genet Genomics. 2001;265:1076-86 pubmed
- Kramer A, Mulhauser F, Wersig C, Gröning K, Bilbe G. Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae. RNA. 1995;1:260-72 pubmed..Binding studies with truncated derivatives of SF3a120 revealed that the SURP domains function in binding to SF3a60, whereas a region of 130 amino acids C-terminal to these domains is essential for contacts with SF3a66. ..
- Jónsson Z, Hindges R, Hubscher U. Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen. EMBO J. 1998;17:2412-25 pubmed
- Wiederkehr A, Avaro S, Prescianotto Baschong C, Haguenauer Tsapis R, Riezman H. The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae. J Cell Biol. 2000;149:397-410 pubmed..cerevisiae and that a major fraction of internalized FM4-64 is secreted back into the medium within a few minutes. The rcy1Delta mutant is strongly defective in recycling. ..
- Bosson R, Jaquenoud M, Conzelmann A. GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor. Mol Biol Cell. 2006;17:2636-45 pubmed..GUP1 is highly conserved among fungi and protozoa and the gup1Delta phenotype is partially corrected by GUP1 homologues of Aspergillus fumigatus and Trypanosoma cruzi. ..
- Bosson R, Guillas I, Vionnet C, Roubaty C, Conzelmann A. Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro. Eukaryot Cell. 2009;8:306-14 pubmed publisher..Thus, [(3)H]DHS cannot be incorporated into proteins in the absence of acyl-CoA. ..
- Aymoz D, Wosika V, Durandau E, Pelet S. Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters. Nat Commun. 2016;7:11304 pubmed publisher..They display distinct expression dynamics giving rise to strikingly different instantaneous expression noise. ..
- Sutterlin C, Doering T, Schimmöller F, Schroder S, Riezman H. Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast. J Cell Sci. 1997;110 ( Pt 21):2703-14 pubmed..In both mutants, GPI-anchor attachment is normal and in ret1-1 cells, the GPI-anchors are remodeled with ceramide to the same extent as in wild-type cells. ..
- Helliwell S, Schmidt A, Ohya Y, Hall M. The Rho1 effector Pkc1, but not Bni1, mediates signalling from Tor2 to the actin cytoskeleton. Curr Biol. 1998;8:1211-4 pubmed..Thus, Tor2 signals to the actin cytoskeleton via Rho1, Pkc1 and the cell integrity MAP kinase cascade. ..
- Burger F, Daugeron M, Linder P. Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis. Nucleic Acids Res. 2000;28:2315-23 pubmed..8S mature rRNAs, thus explaining the final deficit in 60S subunit and the formation of half-mer polysomes. Consistent with a direct role in ribosome biogenesis, Dbp10p was found to be located predominantly in the nucleolus. ..
- Friant S, Lombardi R, Schmelzle T, Hall M, Riezman H. Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J. 2001;20:6783-92 pubmed..The Pkc1p kinase that is phosphorylated by Pkh1/2p kinases and plays a role in endocytosis was identified as one of the downstream effectors of this signaling cascade. ..
- Köffel R, Schneiter R. Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:1018-25 pubmed..These data thus provide the first link between fat degradation and the transcriptional control of lipase activity in yeast. ..
- Marques A, Vinckenbosch N, Brawand D, Kaessmann H. Functional diversification of duplicate genes through subcellular adaptation of encoded proteins. Genome Biol. 2008;9:R54 pubmed publisher..We conclude that protein subcellular adaptation represents a common mechanism for the functional diversification of duplicate genes. ..
- Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
- Strub K, Fornallaz M, Bui N. The Alu domain homolog of the yeast signal recognition particle consists of an Srp14p homodimer and a yeast-specific RNA structure. RNA. 1999;5:1333-47 pubmed..The alignment of the three yeast SRP RNAs known to date suggests a common structure for the putative elongation arrest domain of all three organisms. ..
- Zanolari B, Friant S, Funato K, Sutterlin C, Stevenson B, Riezman H. Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae. EMBO J. 2000;19:2824-33 pubmed..This is the first case of a physiological role for sphingoid base synthesis, other than as a precursor for ceramide or phosphorylated sphingoid base synthesis. ..
- Interthal H, Heyer W. MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet. 2000;263:812-27 pubmed..We hypothesize that Mus81p plays a role in the recognition and/or processing of certain types of DNA damage (caused by UV and MMS) during repair or tolerance processes involving the recombinational repair pathway. ..
- Lucht J, Mauch Mani B, Steiner H, Metraux J, Ryals J, Hohn B. Pathogen stress increases somatic recombination frequency in Arabidopsis. Nat Genet. 2002;30:311-4 pubmed..The increased genetic flexibility might facilitate evolutionary adaptation of plant populations to stressful environments. ..
- Dichtl B, Blank D, Sadowski M, Hubner W, Weiser S, Keller W. Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. EMBO J. 2002;21:4125-35 pubmed..We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination. ..
- Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed..Thus, the distinct TOR complexes account for the diversity, specificity, and selective rapamycin inhibition of TOR signaling. TORC1 and possibly TORC2 are conserved from yeast to man. ..
- Vionnet C, Roubaty C, Ejsing C, Knudsen J, Conzelmann A. Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors. J Biol Chem. 2011;286:6769-79 pubmed publisher..Furthermore, mannosylation of PI? is required for the survival of sphingolipid-deficient strains, which depend on SLC1-1. In contrast to lcb1? SLC1-1, 4? SLC1-1 cells grow at 37 °C but remain thermosensitive at 44 °C. ..