Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Sweden
Topic: saccharomyces cerevisiae proteins
Publications140 found, 100 shown here
- Otero J, Cimini D, Patil K, Poulsen S, Olsson L, Nielsen J. Industrial systems biology of Saccharomyces cerevisiae enables novel succinic acid cell factory. PLoS ONE. 2013;8:e54144 pubmed publisher..cerevisiae, and hence show proof of concept that this is a potentially attractive cell factory for over-producing different platform chemicals. ..
- Ahmadpour D, Geijer C, Tamás M, Lindkvist Petersson K, Hohmann S. Yeast reveals unexpected roles and regulatory features of aquaporins and aquaglyceroporins. Biochim Biophys Acta. 2014;1840:1482-91 pubmed publisher..This article is part of a Special Issue entitled Aquaporins. ..
- Schagerlöf U, Elmlund H, Gakh O, Nordlund G, Hebert H, Lindahl M, et al. Structural basis of the iron storage function of frataxin from single-particle reconstruction of the iron-loaded oligomer. Biochemistry. 2008;47:4948-54 pubmed publisher..These include the overall organization of the oligomers, the way they are stabilized, and the mechanisms of iron core nucleation. ..
- Heessen S, Dantuma N, Tessarz P, Jellne M, Masucci M. Inhibition of ubiquitin/proteasome-dependent proteolysis in Saccharomyces cerevisiae by a Gly-Ala repeat. FEBS Lett. 2003;555:397-404 pubmed..The conserved effect of the GA repeat in yeast opens the possibility for the use of genetic screens to unravel its mode of action. ..
- Bilsland E, Molin C, Swaminathan S, Ramne A, Sunnerhagen P. Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance. Mol Microbiol. 2004;53:1743-56 pubmed..Zrc1 co-immunoprecipitates with Rck2, and ZRC1 is genetically downstream of RCK2. These data connect activation of the Hog1 MAPK cascade with effectors having a role in oxidative stress resistance. ..
- Henricsson C, de Jesus Ferreira M, Hedfalk K, Elbing K, Larsson C, Bill R, et al. Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations. Appl Environ Microbiol. 2005;71:6185-92 pubmed
- Petersson A, Almeida J, Modig T, Karhumaa K, Hahn Hägerdal B, Gorwa Grauslund M, et al. A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance. Yeast. 2006;23:455-64 pubmed..Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity...
- Wadskog I, Forsmark A, Rossi G, Konopka C, Oyen M, Goksör M, et al. The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface. Mol Biol Cell. 2006;17:4988-5003 pubmed..We propose a model in which blocked exocytic sorting in sro7Delta mutants, gives rise to quality control-mediated routing of Ena1p to the vacuole. ..
- Karlberg T, Schagerlöf U, Gakh O, Park S, Ryde U, Lindahl M, et al. The structures of frataxin oligomers reveal the mechanism for the delivery and detoxification of iron. Structure. 2006;14:1535-46 pubmed..The data reveal how stepwise assembly provides frataxin with the structural flexibility to perform two functions: metal delivery and detoxification. ..
- García Salcedo R, Lubitz T, Beltran G, Elbing K, Tian Y, Frey S, et al. Glucose de-repression by yeast AMP-activated protein kinase SNF1 is controlled via at least two independent steps. FEBS J. 2014;281:1901-17 pubmed publisher..Hence it appears that glucose de-repression via Snf1-Mig1 is regulated by glucose via at least two independent steps: the control of activation of the Snf1 kinase and directing active Snf1 to inactivating its target Mig1. ..
- Helgstrand C, Hasan M, Uysal H, Haeggstrom J, Thunnissen M. A leukotriene A4 hydrolase-related aminopeptidase from yeast undergoes induced fit upon inhibitor binding. J Mol Biol. 2011;406:120-34 pubmed publisher..Inasmuch as bestatin is a structural analogue of a leucyl dipeptide and may be regarded as a transition state mimic, our results imply that the enzyme undergoes induced fit during substrate binding and turnover. ..
- Zhu X, Zhang Y, Bjornsdottir G, Liu Z, Quan A, Costanzo M, et al. Histone modifications influence mediator interactions with chromatin. Nucleic Acids Res. 2011;39:8342-54 pubmed publisher..Our studies show that chromatin structure and the acetylation state of histones are intimately connected to Mediator localization. ..
- Sandager L, Gustavsson M, Stahl U, Dahlqvist A, Wiberg E, Banas A, et al. Storage lipid synthesis is non-essential in yeast. J Biol Chem. 2002;277:6478-82 pubmed..The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies. We conclude that neither storage lipids nor lipid bodies are essential for growth in yeast. ..
- Ramne A, Bilsland Marchesan E, Erickson S, Sunnerhagen P. The protein kinases Rck1 and Rck2 inhibit meiosis in budding yeast. Mol Gen Genet. 2000;263:253-61 pubmed..There is a transient shift in the relative abundance of the two RCK2 transcripts in meiotic cells. We propose that one function of Rck1 and Rck2 is to inhibit meiosis. ..
- Hallberg M, Polozkov G, Hu G, Beve J, Gustafsson C, Ronne H, et al. Site-specific Srb10-dependent phosphorylation of the yeast Mediator subunit Med2 regulates gene expression from the 2-microm plasmid. Proc Natl Acad Sci U S A. 2004;101:3370-5 pubmed..Combined with previous studies on the effects of SRB10/SRB11 deletions, our data suggest that posttranslational modifications of Mediator subunits are important for regulation of gene expression. ..
- Hafizi S, Dahlback B. Gas6 and protein S. Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily. FEBS J. 2006;273:5231-44 pubmed..Further knowledge of this exquisite ligand-receptor system and the circumstances of its activation should provide the basis for development of novel therapies for the above diseases. ..
- Laadan B, Almeida J, Radstrom P, Hahn Hägerdal B, Gorwa Grauslund M. Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae. Yeast. 2008;25:191-8 pubmed publisher..Increased biomass production and HMF conversion rate were achieved in a CEN.PK S. cerevisiae strain overexpressing the mutated ADH1 gene. ..
- Lundkvist P, Jupiter S, Segerstolpe A, Osheim Y, Beyer A, Wieslander L. Mrd1p is required for release of base-paired U3 snoRNA within the preribosomal complex. Mol Cell Biol. 2009;29:5763-74 pubmed publisher..Mrd1p therefore coordinates key events in biosynthesis of small ribosome subunits. ..
- Kota J, Melin Larsson M, Ljungdahl P, Forsberg H. Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae. Genetics. 2007;175:1681-94 pubmed
- Danielsson J, Liljedahl L, Bárány Wallje E, Sønderby P, Kristensen L, Martinez Yamout M, et al. The intrinsically disordered RNR inhibitor Sml1 is a dynamic dimer. Biochemistry. 2008;47:13428-37 pubmed..We propose that this molecular shielding and protection of vital functional structures from degradation by functionally unimportant sites may be a general attribute of other natively disordered proteins. ..
- Ranatunga W, Gakh O, Galeano B, Smith D, SÃ¶derberg C, Al Karadaghi S, et al. Architecture of the Yeast Mitochondrial Iron-Sulfur Cluster Assembly Machinery: THE SUB-COMPLEX FORMED BY THE IRON DONOR, Yfh1 PROTEIN, AND THE SCAFFOLD, Isu1 PROTEIN. J Biol Chem. 2016;291:10378-98 pubmed publisher..We suggest that this architecture is suitable to ensure concerted and protected transfer of potentially toxic iron and sulfur atoms to Isu1 during Fe-S cluster assembly. ..
- Molin M, Blomberg A. Dihydroxyacetone detoxification in Saccharomyces cerevisiae involves formaldehyde dissimilation. Mol Microbiol. 2006;60:925-38 pubmed..We conclude that mechanisms for DHA growth and detoxification appear complex and that the evolutionary strive to minimize detrimental effects of this intracellular metabolite links to both formaldehyde and glutathione metabolism. ..
- Furukawa K, Randhawa A, Kaur H, Mondal A, Hohmann S. Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinase. FEBS Lett. 2012;586:2417-22 pubmed publisher..Our results explain the different reasons for fludioxonil resistance conferred by its deletion and missense mutation. ..
- Warringer J, Hult M, Regot S, Posas F, Sunnerhagen P. The HOG pathway dictates the short-term translational response after hyperosmotic shock. Mol Biol Cell. 2010;21:3080-92 pubmed publisher..Our results show the importance of translational control for fine tuning of the adaptive responses. ..
- Johansson M, Chen X, Milanova S, Santos C, Petranovic D. PUFA-induced cell death is mediated by Yca1p-dependent and -independent pathways, and is reduced by vitamin C in yeast. FEMS Yeast Res. 2016;16:fow007 pubmed publisher..When deleting YCA1, the caspase-like activity and the oxidative stress decreased and although the lifespan was slightly prolonged, the phenotype could not be fully reversed, pointing that Yca1p was not the main executor of cell death. ..
- Tanimoto K, Makino Y, Pereira T, Poellinger L. Mechanism of regulation of the hypoxia-inducible factor-1 alpha by the von Hippel-Lindau tumor suppressor protein. EMBO J. 2000;19:4298-309 pubmed..VHL was not released from HIF-1 alpha during this process. Finally, stabilization of HIF-1 alpha protein levels per se did not totally bypass the need of the hypoxic signal for generating the transactivation response. ..
- Boban M, Ljungdahl P. Dal81 enhances Stp1- and Stp2-dependent transcription necessitating negative modulation by inner nuclear membrane protein Asi1 in Saccharomyces cerevisiae. Genetics. 2007;176:2087-97 pubmed..Conversely, the high levels of processed Stp1 and Stp2 that accumulate in the nucleus of induced cells activate transcription in the absence of Dal81. ..
- Szilagyi Z, Gustafsson C. Emerging roles of Cdk8 in cell cycle control. Biochim Biophys Acta. 2013;1829:916-20 pubmed publisher..We here discuss recent advances in our understanding of Cdk8 function and a possible role for Mediator as a hub for integrating transcription regulation with cell cycle progression. ..
- Then Bergh F, Flinn E, Svaren J, Wright A, Hörz W. Comparison of nucleosome remodeling by the yeast transcription factor Pho4 and the glucocorticoid receptor. J Biol Chem. 2000;275:9035-42 pubmed..Our results show that the DNA-binding domains of the two factors play critical roles in determining how chromatin structure is modified during promoter activation. ..
- Panagopoulos I, Fioretos T, Isaksson M, Samuelsson U, Billström R, Strombeck B, et al. Fusion of the MORF and CBP genes in acute myeloid leukemia with the t(10;16)(q22;p13). Hum Mol Genet. 2001;10:395-404 pubmed..Since both fusion transcripts were present, their exact role in the leukemogenic process remains to be elucidated. ..
- Biterova E, Svärd M, Possner D, Guy J. The crystal structure of the lumenal domain of Erv41p, a protein involved in transport between the endoplasmic reticulum and Golgi apparatus. J Mol Biol. 2013;425:2208-18 pubmed publisher..This structure, the first of Erv41p or any of its homologues, provides a new starting point for studies of the roles of Erv41p and its interaction partners in the eukaryotic secretory pathway. ..
- Sengottaiyan P, Petrlova J, Lagerstedt J, Ruiz Pavón L, Budamagunta M, Voss J, et al. Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89. Biochem Biophys Res Commun. 2013;436:551-6 pubmed publisher..The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate. ..
- Bilsland Marchesan E, Arino J, Saito H, Sunnerhagen P, Posas F. Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1. Mol Cell Biol. 2000;20:3887-95 pubmed..Taken together, our data suggest that Rck2 acts downstream of Hog1, controlling a subset of the responses induced by the MAPK upon osmotic stress. ..
- Nordlund M, Johansson J, von Pawel Rammingen U, Byström A. Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae. RNA. 2000;6:844-60 pubmed..The purified yNucR endo-exonuclease has been reported to have an NH2-D-E-K-N-L motif, which is not found in the Trm2p. Therefore, we suggest that the yNucR endo-exonuclease is encoded by a gene other than TRM2. ..
- Martinez P, Ljungdahl P. The SHR3 homologue from S. pombe demonstrates a conserved function of ER packaging chaperones. J Cell Sci. 2000;113 Pt 23:4351-62 pubmed
- Oberg C, Li J, Pauley A, Wolf E, Gurney M, Lendahl U. The Notch intracellular domain is ubiquitinated and negatively regulated by the mammalian Sel-10 homolog. J Biol Chem. 2001;276:35847-53 pubmed..In conclusion, these data reveal the importance of ubiquitination and proteasome-mediated degradation for the activity and turnover of Notch ICs, and demonstrate that mSel-10 plays a key role in this process. ..
- Linder T, Gustafsson C. The Soh1/MED31 protein is an ancient component of Schizosaccharomyces pombe and Saccharomyces cerevisiae Mediator. J Biol Chem. 2004;279:49455-9 pubmed..Bioinformatic analysis traces the Soh1/MED31 family of Mediator subunits to the point of major eukaryotic divergence, before the appearance of the canonical heptapeptide repeat structure of the RNA polymerase II C-terminal domain. ..
- Furukawa K, Sidoux Walter F, Hohmann S. Expression of the yeast aquaporin Aqy2 affects cell surface properties under the control of osmoregulatory and morphogenic signalling pathways. Mol Microbiol. 2009;74:1272-86 pubmed publisher..Our observations suggest a potential link between aquaporins and cell surface properties, and relate to the proposed role of mammalian aquaporins in tumour cell migration and invasion. ..
- Hu G, Ronne H. Yeast BTF3 protein is encoded by duplicated genes and inhibits the expression of some genes in vivo. Nucleic Acids Res. 1994;22:2740-3 pubmed..We conclude that Btt1p and Egd1p have redundant functions in vivo, and that they exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II. ..
- Lundin M, Nehlin J, Ronne H. Importance of a flanking AT-rich region in target site recognition by the GC box-binding zinc finger protein MIG1. Mol Cell Biol. 1994;14:1979-85 pubmed..We conclude that DNA binding by a simple zinc finger protein such as MIG1 can involve both recognition of the GC box and flanking sequence preferences that may reflect local DNA bendability. ..
- Forsberg H, Hammar M, Andreasson C, Moliner A, Ljungdahl P. Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae. Genetics. 2001;158:973-88 pubmed..The results from genetic and phenotypic analysis indicate that SPS sensor-initiated signals function positively to facilitate amino acid uptake and that two independent ubiquitin-mediated processes negatively modulate amino acid uptake. ..
- Andréasson C, Neve E, Ljungdahl P. Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast. Yeast. 2004;21:193-9 pubmed..These findings have implications for the interpretation of studies using AzC to characterize nitrogen source-dependent regulation of amino acid uptake and of post-Golgi targeting and localization of amino acid permeases in yeast. ..
- Lu J, Huang B, Esberg A, Johansson M, Byström A. The Kluyveromyces lactis gamma-toxin targets tRNA anticodons. RNA. 2005;11:1648-54 pubmed..The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA. ..
- Andersson C, Isaksson A, Gustafsson M. Bayesian detection of periodic mRNA time profiles without use of training examples. BMC Bioinformatics. 2006;7:63 pubmed..Results from applying the detector to mRNA expression time profiles from S. cerevisiae include several new findings that deserve further experimental studies. ..
- Thorsen M, Perrone G, Kristiansson E, Traini M, Ye T, Dawes I, et al. Genetic basis of arsenite and cadmium tolerance in Saccharomyces cerevisiae. BMC Genomics. 2009;10:105 pubmed publisher..Since many of the yeast genes that confer tolerance to these agents have homologues in humans, similar biological processes may act in yeast and humans to prevent metal toxicity and carcinogenesis. ..
- Forsgren M, Attersand A, Lake S, Grunler J, Swiezewska E, Dallner G, et al. Isolation and functional expression of human COQ2, a gene encoding a polyprenyl transferase involved in the synthesis of CoQ. Biochem J. 2004;382:519-26 pubmed..CoQ formed when cells were incubated with labelled decaprenyl pyrophosphate and nonaprenyl pyrophosphate, showing that the human enzyme is active and that it participates in the biosynthesis of CoQ. ..
- Lagerstedt J, Reeve I, Voss J, Persson B. Structure and function of the GTP binding protein Gtr1 and its role in phosphate transport in Saccharomyces cerevisiae. Biochemistry. 2005;44:511-7 pubmed..In contrast, the spectra obtained for the C-terminal part (residues 186-310) displayed no evidence of conformational changes upon GTP addition. ..
- Thorsen M, Di Y, Tängemo C, Morillas M, Ahmadpour D, Van der Does C, et al. The MAPK Hog1p modulates Fps1p-dependent arsenite uptake and tolerance in yeast. Mol Biol Cell. 2006;17:4400-10 pubmed..Understanding how arsenite/antimonite uptake and toxicity is modulated may prove of value for their use in medical therapy. ..
- Söderberg C, Shkumatov A, Rajan S, Gakh O, Svergun D, Isaya G, et al. Oligomerization propensity and flexibility of yeast frataxin studied by X-ray crystallography and small-angle X-ray scattering. J Mol Biol. 2011;414:783-97 pubmed publisher..They also indicate that different mechanisms may control oligomer stability and oligomerization in vivo. ..
- Geijer C, Medrala Klein D, Petelenz Kurdziel E, Ericsson A, Smedh M, Andersson M, et al. Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery. FEBS J. 2013;280:3854-67 pubmed publisher..Furthermore, we found that the period of Hog1 nuclear residence affects the amplitude of the transcriptional response rather than the spectrum of responsive genes. ..
- Aström S, Byström A. Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination. Cell. 1994;79:535-46 pubmed..The modification enzyme was shown to recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs. ..
- Liljas A, Garber M. Ribosomal proteins and elongation factors. Curr Opin Struct Biol. 1995;5:721-7 pubmed..In addition, the complex of EF-Tu with a GTP analogue and Phe-tRNA(Phe) has a structure that overlaps exceedingly well with that of EF-G-GDP. These findings obviously have functional implications. ..
- Laizé V, Tacnet F, Ripoche P, Hohmann S. Polymorphism of Saccharomyces cerevisiae aquaporins. Yeast. 2000;16:897-903 pubmed..We conclude that natural and industrial conditions provide selective pressure to maintain AQY1 but apparently not AQY2. ..
- Fristedt U, van der Rest M, Poolman B, Konings W, Persson B. Studies of cytochrome c oxidase-driven H(+)-coupled phosphate transport catalyzed by the Saccharomyces cerevisiae Pho84 permease in coreconstituted vesicles. Biochemistry. 1999;38:16010-5 pubmed..The metal dependency of transport indicates that a metal-phosphate complex is the translocated substrate. ..
- Kim H, von Heijne G, Nilsson I. Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex. J Biol Chem. 2005;280:20261-7 pubmed..Our results suggest that STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation. ..
- Alikhani N, Berglund A, Engmann T, Spånning E, Vögtle F, Pavlov P, et al. Targeting capacity and conservation of PreP homologues localization in mitochondria of different species. J Mol Biol. 2011;410:400-10 pubmed publisher..Moreover, complementation studies in yeast show that native AtPreP restores the growth phenotype of yeast cells lacking Cym1, demonstrating functional conservation. ..
- Tyo K, Liu Z, Petranovic D, Nielsen J. Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress. BMC Biol. 2012;10:16 pubmed publisher..This molecular insight has direct implications on engineering a broad range of recombinant proteins for secretion and provides potential hypotheses for the root causes of several secretory-associated diseases. ..
- Jacobson T, Navarrete C, Sharma S, Sideri T, Ibstedt S, Priya S, et al. Arsenite interferes with protein folding and triggers formation of protein aggregates in yeast. J Cell Sci. 2012;125:5073-83 pubmed publisher..Our findings describe a novel mechanism of toxicity that may explain the suggested role of this metalloid in the etiology and pathogenesis of protein folding disorders associated with arsenic poisoning. ..
- Gruschke S, Römpler K, Hildenbeutel M, Kehrein K, Kühl I, Bonnefoy N, et al. The Cbp3-Cbp6 complex coordinates cytochrome b synthesis with bc(1) complex assembly in yeast mitochondria. J Cell Biol. 2012;199:137-50 pubmed publisher..This feedback loop regulates protein synthesis at the inner mitochondrial membrane by directly monitoring the efficiency of bc(1) complex assembly. ..
- Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J. Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng. 2013;15:48-54 pubmed publisher..This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products. ..
- Gowda N, Kandasamy G, Froehlich M, Dohmen R, Andréasson C. Hsp70 nucleotide exchange factor Fes1 is essential for ubiquitin-dependent degradation of misfolded cytosolic proteins. Proc Natl Acad Sci U S A. 2013;110:5975-80 pubmed publisher..Our findings reveal that Hsp70 direct proteins toward either folding or degradation by using distinct nucleotide exchange factors. ..
- Andersson V, Hanzén S, Liu B, Molin M, Nystrom T. Enhancing protein disaggregation restores proteasome activity in aged cells. Aging (Albany NY). 2013;5:802-12 pubmed..The data supports the existence of a negative feedback loop that accelerates aging by exacerbating proteostatic decline once misfolded and aggregation-prone proteins reach a critical level. ..
- Lagerstedt J, Voss J, Wieslander A, Persson B. Structural modeling of dual-affinity purified Pho84 phosphate transporter. FEBS Lett. 2004;578:262-8 pubmed..The line shape from labels located at both positions is consistent with the structural environment predicted by the template-generated model, thus supporting the model. ..
- Bernander R. Chromosome replication, nucleoid segregation and cell division in archaea. Trends Microbiol. 2000;8:278-83 pubmed..The evolutionary and functional relationships between the archaeal Cdc6 protein and bacterial and eukaryal replication initiation factors are discussed. ..
- Garcia Sanchez R, Hahn Hägerdal B, Gorwa Grauslund M. PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae. Microb Cell Fact. 2010;9:40 pubmed publisher..This observation is of particular importance for the future development of stable industrial strains with enhanced PGM activity. ..
- Larsson K, Bohl F, Sjöström I, Akhtar N, Strand D, Mechler B, et al. The Saccharomyces cerevisiae SOP1 and SOP2 genes, which act in cation homeostasis, can be functionally substituted by the Drosophila lethal(2)giant larvae tumor suppressor gene. J Biol Chem. 1998;273:33610-8 pubmed
- Aström S, Nordlund M, Erickson F, Hannig E, Byström A. Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae. Mol Gen Genet. 1999;261:967-76 pubmed
- Bill R, Hedfalk K, Karlgren S, Mullins J, Rydstrom J, Hohmann S. Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1p. J Biol Chem. 2001;276:36543-9 pubmed..We discuss the fact that this may accommodate the divergent NPA motifs of Fps1p and that the different pore structures of Fps1p and GlpF may reflect the physiological roles of the two glycerol facilitators. ..
- Chabes A, Georgieva B, Domkin V, Zhao X, Rothstein R, Thelander L. Survival of DNA damage in yeast directly depends on increased dNTP levels allowed by relaxed feedback inhibition of ribonucleotide reductase. Cell. 2003;112:391-401 pubmed..We propose that increased survival and mutation rates result from more efficient translesion DNA synthesis at elevated dNTP concentrations. ..
- Heessen S, Masucci M, Dantuma N. The UBA2 domain functions as an intrinsic stabilization signal that protects Rad23 from proteasomal degradation. Mol Cell. 2005;18:225-35 pubmed..Thus, the UBA2 domain of Rad23 is an evolutionarily conserved stabilization signal that allows Rad23 to interact with the proteasome without facing destruction. ..
- Beve J, Hu G, Myers L, Balciunas D, Werngren O, Hultenby K, et al. The structural and functional role of Med5 in the yeast Mediator tail module. J Biol Chem. 2005;280:41366-72 pubmed..We discuss a possible role for Med5 in coordinating nuclear and mitochondrial gene transcription. ..
- Andréasson C, Heessen S, Ljungdahl P. Regulation of transcription factor latency by receptor-activated proteolysis. Genes Dev. 2006;20:1563-8 pubmed..Our findings demonstrate that gene expression can be controlled by regulating the enzymatic activity of an intracellular endoprotease. ..
- Rosenblad M, López M, Piccinelli P, Samuelsson T. Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes. Nucleic Acids Res. 2006;34:5145-56 pubmed..We suggest that this motif is a binding site for the Pop3/Rpp38 proteins and we discuss other structural features of the RNA subunit and possible relationships to the protein subunit repertoire. ..
- Kota J, Gilstring C, Ljungdahl P. Membrane chaperone Shr3 assists in folding amino acid permeases preventing precocious ERAD. J Cell Biol. 2007;176:617-28 pubmed..Consequently, in cells with impaired ERAD, AAPs are able to attain functional conformations independent of Shr3. These findings illustrate that folding and degradation are tightly coupled processes during membrane protein biogenesis. ..
- Carter S, Vigasová D, Chen J, Chovanec M, Aström S. Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism. Proc Natl Acad Sci U S A. 2009;106:12037-42 pubmed publisher..This unexpected link between NHEJ and HR components may represent cross-talk between DSB repair pathways to ensure efficient repair. ..
- Shaikhibrahim Z, Rahaman H, Wittung Stafshede P, Bjorklund S. Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation. Proc Natl Acad Sci U S A. 2009;106:20728-33 pubmed publisher..However, the concurrent presence of all three protein subunits during the renaturation process is required for proper folding and trimer complex formation. ..
- Nissan T, Rajyaguru P, She M, Song H, Parker R. Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms. Mol Cell. 2010;39:773-83 pubmed publisher..Moreover, Pat1 is identified as critical in mRNA decay by first inhibiting translation initiation, then serving as a scaffold to recruit components of the decapping complex, and finally activating Dcp2. ..
- Geijer C, Ahmadpour D, Palmgren M, Filipsson C, Klein D, Tamás M, et al. Yeast aquaglyceroporins use the transmembrane core to restrict glycerol transport. J Biol Chem. 2012;287:23562-70 pubmed publisher..This mechanism enables yeast cells to fine-tune intracellular glycerol levels at a wide range of extracellular osmolarities. ..
- Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed..These findings suggest that Mso1p is a component of the secretory vesicle docking complex whose function is closely associated with that of Sec1p. ..
- Pedrajas J, Miranda Vizuete A, Javanmardy N, Gustafsson J, Spyrou G. Mitochondria of Saccharomyces cerevisiae contain one-conserved cysteine type peroxiredoxin with thioredoxin peroxidase activity. J Biol Chem. 2000;275:16296-301 pubmed..Finally, exposure of null Prx1p mutant cells to oxidant conditions reveals an important role of the mitochondrial 1-Cys Prx in protection against oxidative stress. ..
- Ericsson U, Nordlund P, Hallberg B. X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold. FEBS Lett. 2004;565:59-64 pubmed..The catalytic domain has a fold similar to that of the catalytic domains of previously characterised pseudouridine synthases, whereas the insertion domain displays a novel fold. ..
- Di Y, Tamás M. Regulation of the arsenic-responsive transcription factor Yap8p involves the ubiquitin-proteasome pathway. J Cell Sci. 2007;120:256-64 pubmed..In this way, regulated degradation contributes to Yap8p control. ..
- Linder T, Gustafsson C. Molecular phylogenetics of ascomycotal adhesins--a novel family of putative cell-surface adhesive proteins in fission yeasts. Fungal Genet Biol. 2008;45:485-97 pubmed..Further sequence analysis identified several putative adhesins in the sub-phylum of Pezizomycotina, where only a few adhesins have been described to date. ..
- Molin C, Jauhiainen A, Warringer J, Nerman O, Sunnerhagen P. mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress. RNA. 2009;15:600-14 pubmed publisher..Conversely, stabilization of stress-repressed mRNAs permits their rapid accumulation in the recovery phase. Our results show that mRNA turnover is coordinated with transcriptional induction. ..
- Siewers V, San Bento R, Nielsen J. Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisiae. Biotechnol Bioeng. 2010;106:841-4 pubmed publisher..This opens the possibility of using yeast as a eukaryotic platform for fast assessment of new module combinations for the development of novel NRP compounds. ..
- Samyn D, Ruiz Pavón L, Andersson M, Popova Y, Thevelein J, Persson B. Mutational analysis of putative phosphate- and proton-binding sites in the Saccharomyces cerevisiae Pho84 phosphate:H(+) transceptor and its effect on signalling to the PKA and PHO pathways. Biochem J. 2012;445:413-22 pubmed publisher