Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Japan
Topic: saccharomyces cerevisiae proteins
Publications153 found, 100 shown here
- Nakamura A, Fukuda A, Sakai S, Tanaka Y. Molecular cloning, functional expression and subcellular localization of two putative vacuolar voltage-gated chloride channels in rice (Oryza sativa L.). Plant Cell Physiol. 2006;47:32-42 pubmed publisher..We isolated loss-of-function mutants of both genes from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17, in the exon region, and found inhibition of growth at all life stages...
- Yamada H, Horigome C, Okada T, Shirai C, Mizuta K. Yeast Rrp14p is a nucleolar protein involved in both ribosome biogenesis and cell polarity. RNA. 2007;13:1977-87 pubmed..These results suggest that Rrp14p has dual functions in ribosome synthesis and polarized cell growth. ..
- Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M. The signaling pathways underlying starvation-induced upregulation of Î±-mannosidase Ams1 in Saccharomyces cerevisiae. Biochim Biophys Acta. 2016;1860:1192-201 pubmed publisher..The signaling molecules responsible for regulation of Ams1 were also clarified. ..
- Fujita A, Misumi Y, Ikehara Y, Kobayashi H. The yeast SFL2 gene may be necessary for mating-type control. Gene. 1992;112:85-90 pubmed..However, there is no significant difference in the levels of the MAT alpha 2 and MATa1 transcripts. These results suggest that the SFL2 gene product may be necessary for alpha 2 and a1-alpha 2 repression. ..
- Sugimoto K, Sakamoto Y, Takahashi O, Matsumoto K. HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae. Nucleic Acids Res. 1995;23:3493-500 pubmed..HYS2 encodes a 55 kDa protein that is essential for viability at all temperatures. Taken together, these data suggest that Hys2 plays a role in DNA replication. ..
- Johzuka K, Ogawa H. Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae. Genetics. 1995;139:1521-32 pubmed..Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth. ..
- Ogawa N, Noguchi K, Sawai H, Yamashita Y, Yompakdee C, Oshima Y. Functional domains of Pho81p, an inhibitor of Pho85p protein kinase, in the transduction pathway of Pi signals in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:997-1004 pubmed..An in vitro assay showed that a glutathione S-transferase-Pho81p fusion protein inhibits the Pho85p protein kinase. Association of Pho81p with Pho85p or with the Pho80p-Pho85p complex was demonstrated by the two-hybrid system. ..
- Ono Y, Ohno M, Shimura Y. Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22. Mol Cell Biol. 1994;14:7611-20 pubmed..We could show that HRH1 can interact in vitro and in the yeast two-hybrid system with members of the SR protein family through its RS domain. We speculate that HRH1 might be targeted to the spliceosome through this interaction. ..
- Takeuchi J, Okada M, Toh e A, Kikuchi Y. The SMS1 gene encoding a serine-rich transmembrane protein suppresses the temperature sensitivity of the htr1 disruptant in Saccharomyces cerevisiae. Biochim Biophys Acta. 1995;1260:94-6 pubmed..The predicted gene product contains a serine-rich domain followed by a putative transmembrane region. The SMS1 gene was physically and genetically mapped in the region near cdc3 on chromosome XII R. ..
- Chiba H, Muramatsu M, Nomoto A, Kato H. Two human homologues of Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma are transcriptional coactivators cooperating with the estrogen receptor and the retinoic acid receptor. Nucleic Acids Res. 1994;22:1815-20 pubmed..No enhancement was observed for promoters which do not respond to nuclear receptors. We suggest that global transcriptional coactivators equivalent to the yeast SWI/SNF complex exist in mammalian cells. ..
- Kito M, Seog D, Igarashi K, Kambe Honjo H, Yoda K, Yamasaki M. Calcium and SLY genes suppress the temperature-sensitive secretion defect of Saccharomyces cerevisiae uso1 mutant. Biochem Biophys Res Commun. 1996;220:653-7 pubmed..The common phenotype and suppression of the mutants suggest that Uso1 and Ypt1 proteins function in the same process of protein transport, i.e., targeting or fusion of the transport vesicles to the Golgi membrane. ..
- Yamakawa H, Seog D, Yoda K, Yamasaki M, Wakabayashi T. Uso1 protein is a dimer with two globular heads and a long coiled-coil tail. J Struct Biol. 1996;116:356-65 pubmed..5 nm from the globular domain. This is consistent with the predicted positions of interruption. These results give new experimental evidence that Uso1 protein is a dimer and has an alpha-helical coiled-coil tail with two globular heads. ..
- Miyahara K, Hirata D, Miyakawa T. Functional interaction of Isr1, a predicted protein kinase, with the Pkc1 pathway in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 1998;62:1376-80 pubmed..These results suggest that Isr1 functions in an event important for growth in a manner redundant with a Mpk1-independent branch of the Pkc1 signalling pathways. ..
- Nakayama K, Feng Y, Tanaka A, Jigami Y. The involvement of mnn4 and mnn6 mutations in mannosylphosphorylation of O-linked oligosaccharide in yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 1998;1425:255-62 pubmed..The amount of mannosylphosphorylated mannotriose was 7% of total O-linked oligosaccharides (20% of neutral mannotriose) of chitinase in strain mnn1. ..
- Tauchi H. Positional cloning and functional analysis of the gene responsible for Nijmegen breakage syndrome, NBS1. J Radiat Res. 2000;41:9-17 pubmed..Functional analysis of the NBS1 protein is in progress and it should provide further clues to understanding the repair mechanism of radiation-induced DNA double-strand breaks. ..
- Higashibata H, Kikuchi H, Kawarabayasi Y, Matsui I. Helicase and nuclease activities of hyperthermophile Pyrococcus horikoshii Dna2 inhibited by substrates with RNA segments at 5'-end. J Biol Chem. 2003;278:15983-90 pubmed..cerevisiae was reported to be stimulated by RNA segments in the 5'-tail (Bae, S.-H., and Seo, Y. S. (2000) J. Biol. Chem. 38022-38031). ..
- Ohdate H, Lim C, Kokubo T, Matsubara K, Kimata Y, Kohno K. Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth. J Biol Chem. 2003;278:14647-56 pubmed..We found that the transcription of some genes depends on functions possessed by both Tih2p and TBP and that these functions are substantially impaired in the spt15/tih2-ts160 double mutants that confer synthetic growth defects. ..
- Satoh T, Sato K, Kanoh A, Yamashita K, Yamada Y, Igarashi N, et al. Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p. J Biol Chem. 2006;281:10410-9 pubmed
- Morohashi N, Yamamoto Y, Kuwana S, Morita W, Shindo H, Mitchell A, et al. Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome. Eukaryot Cell. 2006;5:1925-33 pubmed..Our results illustrate a useful paradigm for analysis of chromatin structural effects at genomic loci. ..
- Kohzaki H, Murakami Y. [Regulation of chromosomal DNA replication by transcription factors]. Seikagaku. 2007;79:458-62 pubmed
- Horigome C, Okada T, Matsuki K, Mizuta K. A ribosome assembly factor Ebp2p, the yeast homolog of EBNA1-binding protein 2, is involved in the secretory response. Biosci Biotechnol Biochem. 2008;72:1080-6 pubmed..These results suggest that Ebp2p is implicated in the secretory response, including changes in nucleolar architecture. ..
- Goshima T, Nakamura R, Kume K, Okada H, Ichikawa E, Tamura H, et al. Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801. Biosci Biotechnol Biochem. 2016;80:1657-62 pubmed publisher..But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity. ..
- Moriya H, Kasai H, Isono K. Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae. Nucleic Acids Res. 1995;23:595-8 pubmed..The C-terminal region of the hrpA gene product seems to contain an RNA binding motif weakly resembling that of ribosomal protein S1 of E.coli. Disruption of the hrpA gene suggested that it is not essential for the growth of E.coli. ..
- Oka T, Nakano A. Inhibition of GTP hydrolysis by Sar1p causes accumulation of vesicles that are a functional intermediate of the ER-to-Golgi transport in yeast. J Cell Biol. 1994;124:425-34 pubmed
- Tanida I, Takita Y, Hasegawa A, Ohya Y, Anraku Y. Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non-exchangeable intracellular Ca2+ pool cooperatively with calcineurin. FEBS Lett. 1996;379:38-42 pubmed..Given that FK506 inhibits the calcineurin activity, Cls2p likely functions in releasing Ca2+ flux from the endoplasmic reticulum, somehow cooperating with calcineurin. ..
- Tomita A, Towatari M, Tsuzuki S, Hayakawa F, Kosugi H, Tamai K, et al. c-Myb acetylation at the carboxyl-terminal conserved domain by transcriptional co-activator p300. Oncogene. 2000;19:444-51 pubmed..We present a new manner of post-translational modification of the c-Myb protein and the potential significance of the acetylation in c-Myb. ..
- Takahashi Y, Kako K, Ohmura K, Tsumori K, Ohmasa Y, Kashiwabara S, et al. Genomic structure of mouse copper chaperone, COX17. DNA Seq. 2001;12:305-18 pubmed..Besides the GC box, binding sites for NRF-1 and 2 known as specific transcription factors for COX subunits are also localized around the transcription starting site. ..
- Hosoda N, Kobayashi T, Uchida N, Funakoshi Y, Kikuchi Y, Hoshino S, et al. Translation termination factor eRF3 mediates mRNA decay through the regulation of deadenylation. J Biol Chem. 2003;278:38287-91 pubmed..These results indicate that the N-domain of eRF3 mediates mRNA decay by regulating deadenylation in a manner coupled to translation. ..
- Araki T, Uesono Y, Oguchi T, Toh e A. LAS24/KOG1, a component of the TOR complex 1 (TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast. Genes Genet Syst. 2005;80:325-43 pubmed..Consistent with the broad cellular functions exerted by the TOR pathway, we found that Las24p was associated with membranes and was localized at vacuoles, the plasma membrane and small vesicles. ..
- Kawahata M, Masaki K, Fujii T, Iefuji H. Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p. FEMS Yeast Res. 2006;6:924-36 pubmed..Moreover, our quantitative PCR showed that expression of PDR12 increased under acid shock response with lactic acid and decreased under acid adaptation with hydrochloric acid. ..
- Sakurai H, Fukasawa T. Yeast Gal11 and transcription factor IIE function through a common pathway in transcriptional regulation. J Biol Chem. 1997;272:32663-9 pubmed..In a reconstituted cell-free system, Gal11 protein stimulated basal transcription in the presence of wild-type TFIIE. Such a stimulation was not seen in the presence of TFIIE-DeltaC. ..
- Yamamoto K, Miyake H, Kusunoki M, Osaki S. Crystal structures of isomaltase from Saccharomyces cerevisiae and in complex with its competitive inhibitor maltose. FEBS J. 2010;277:4205-14 pubmed publisher..These features of the isomaltase active site pocket prevent isomalto-oligosaccharides from binding to the active site effectively. ..
- Ito T, Koga K, Hemmi H, Yoshimura T. Role of zinc ion for catalytic activity in d-serine dehydratase from Saccharomyces cerevisiae. FEBS J. 2012;279:612-24 pubmed publisher..Our data suggest that DsdSC catalyzes the ?-hydrogen abstraction and hydroxyl group elimination in a concerted fashion. ..
- Ohtoshi A, Miyake T, Arai K, Masai H. Analyses of Saccharomyces cerevisiae Cdc7 kinase point mutants: dominant-negative inhibition of DNA replication on overexpression of kinase-negative Cdc7 proteins. Mol Gen Genet. 1997;254:562-70 pubmed..Our results are consistent with the notion that association of Dbf4 with Cdc7 is essential for the G1 to S transition in S. cerevisiae. ..
- Kishi T, Seno T, Yamao F. Grr1 functions in the ubiquitin pathway in Saccharomyces cerevisiae through association with Skp1. Mol Gen Genet. 1998;257:143-8 pubmed..Furthermore, Grr1 bound Skp1 directly in vitro. These results strongly suggest that Grr1 functions in the ubiquitin pathway through association with Skp1. ..
- Arioka M, Kouhashi M, Yoda K, Takatsuki A, Yamasaki M, Kitamoto K. Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombe. Biosci Biotechnol Biochem. 1998;62:390-2 pubmed..These findings suggest that bfr1+ acts as the most downstream effector of the multidrug resistance conferred by bfr2+ and pap1+ genes. ..
- Kitamura K, Maekawa H, Shimoda C. Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase. Mol Biol Cell. 1998;9:1065-80 pubmed..In these cells, mitosis was inhibited and an extra round of DNA replication occurred. Ste9 regulates G1 progression possibly by controlling the amount of the mitotic cyclin in the G1-phase. ..
- Uesono Y, Toh e A, Kikuchi Y, Araki T, Hachiya T, Watanabe C, et al. Local Anesthetics and Antipsychotic Phenothiazines Interact Nonspecifically with Membranes and Inhibit Hexose Transporters in Yeast. Genetics. 2016;202:997-1012 pubmed publisher..When Hxts are scarce, this preference is lost, thereby mitigating the alleviation by additional glucose. These results provide a mechanism that explains how different compounds induce similar effects based on lipid theory. ..
- Kominami K, Sakata Y, Sakai M, Yamashita I. Protein kinase activity associated with the IME2 gene product, a meiotic inducer in the yeast Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 1993;57:1731-5 pubmed..An Ime2-beta-galactosidase fusion was shown by immunofluorescence microscopy to be localized predominantly to the nucleus, suggesting a nuclear function of Ime2. ..
- Wada Y, Nakamura N, Ohsumi Y, Hirata A. Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae. J Cell Sci. 1997;110 ( Pt 11):1299-306 pubmed..We conclude from these observations that Vam3p is a novel member of syntaxin in the vacuoles and it provides the t-SNARE function in a late step of the vacuolar assembly. ..
- Kitagaki H, Shimoi H, Itoh K. Identification and analysis of a static culture-specific cell wall protein, Tir1p/Srp1p in Saccharomyces cerevisiae. Eur J Biochem. 1997;249:343-9 pubmed..TIR1/SRP1 mRNA was transcribed only in the static culture and its transcription was regulated by the ROX1 repressor. ..
- Maeda K, Inui S, Sanjo H, Sakaguchi N. The gene structure and promoter analysis of mouse lymphocyte signal transduction molecule alpha 4 that is related to the yeast TAP42 involved in a rapamycin-sensitive pathway. Gene. 1998;210:287-95 pubmed
- Kawasaki H, Schiltz L, Chiu R, Itakura K, Taira K, Nakatani Y, et al. ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation. Nature. 2000;405:195-200 pubmed..ATF-2 may represent a new class of sequence-specific factors, which are able to activate transcription by direct effects on chromatin components. ..
- Hoque M, Ishikawa F. Cohesin defects lead to premature sister chromatid separation, kinetochore dysfunction, and spindle-assembly checkpoint activation. J Biol Chem. 2002;277:42306-14 pubmed..These results indicate that Scc1 is essential for the association of kinetochores with microtubules. ..
- Nakamura T, Ohmoto T, Hirata D, Tsuchiya E, Miyakawa T. Genetic evidence for the functional redundancy of the calcineurin- and Mpk1-mediated pathways in the regulation of cellular events important for growth in Saccharomyces cerevisiae. Mol Gen Genet. 1996;251:211-9 pubmed..These results indicated that the calcineurin-mediated and the Mpk1- (Bck1-) mediated signaling pathways act in parallel to regulate functionally redundant cellular events important for growth. ..
- Shobuike T, Sugano S, Yamashita T, Ikeda H. Cloning and characterization of mouse Dhm2 cDNA, a functional homolog of budding yeast SEP1. Gene. 1997;191:161-6 pubmed..Northern analysis revealed that 5.8 kb mRNA corresponding to Dhm2 open reading frame is produced highly in testis. These results strongly suggest that Dhm2p participates in gametogenesis in mouse. ..
- Kamisaka Y, Kimura K, Uemura H, Yamaoka M. Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast. Appl Microbiol Biotechnol. 2013;97:7345-55 pubmed publisher..These results provide a new strategy to engineer S. cerevisiae for optimal lipid production. ..
- Ogawa H, Johzuka K, Nakagawa T, Leem S, Hagihara A. Functions of the yeast meiotic recombination genes, MRE11 and MRE2. Adv Biophys. 1995;31:67-76 pubmed..In conclusion, MRE genes are involved in the initiation of meiotic recombination through the formation of DSBs at recombination hot-spots in S. cerevisiae. ..
- Odani T, Shimma Y, Wang X, Jigami Y. Mannosylphosphate transfer to cell wall mannan is regulated by the transcriptional level of the MNN4 gene in Saccharomyces cerevisiae. FEBS Lett. 1997;420:186-90 pubmed..This type of transcriptional regulation is observed in many stress response genes, implying that mannosylphosphate transfer is involved in the cellular response to a variety of stresses. ..
- Sasaki T, Toh e A, Kikuchi Y. Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Mol Gen Genet. 2000;262:940-8 pubmed..Most of the isolated tmr mutations rescued one of the defects seen in both types of heat stress response in the tom1 mutant. ..
- Mizuno T, Kishimoto T, Shinzato T, Haw R, Chambers A, Wood J, et al. Role of the N-terminal region of Rap1p in the transcriptional activation of glycolytic genes in Saccharomyces cerevisiae. Yeast. 2004;21:851-66 pubmed..These results suggest that one function of the N-terminal region of Rap1p, presumably the BRCT domain, is to facilitate the binding of Gcr1p to the promoter by a protein-protein interaction. ..
- Tomomura M, Imamura Y, Tomomura A, Horiuchi M, Saheki T. Abnormal gene expression and regulation in the liver of jvs mice with systemic carnitine deficiency. Biochim Biophys Acta. 1994;1226:307-14 pubmed..We suggest that elevated AP-1 binding induced by carnitine deficiency is closely connected with the abnormal gene expression in the liver. ..
- Inoue N, Watanabe R, Takeda J, Kinoshita T. PIG-C, one of the three human genes involved in the first step of glycosylphosphatidylinositol biosynthesis is a homologue of Saccharomyces cerevisiae GPI2. Biochem Biophys Res Commun. 1996;226:193-9 pubmed..Since there are several human EST sequences that have homology to GPI1, our results suggest that four genes are involved in the first step of GPI anchor synthesis in mammalian cells. ..
- Kobayashi S, Miyabe S, Izawa S, Inoue Y, Kimura A. Correlation of the OSR/ZRCI gene product and the intracellular glutathione levels in Saccharomyces cerevisiae. Biotechnol Appl Biochem. 1996;23 ( Pt 1):3-6 pubmed..40% compared with that of wild-type cells, whereas it increased 3-fold in yeast overexpressing the OSR gene. Therefore the OSR/ZRCI gene product seems to regulate the intracellular glutathione content. ..
- Tanaka K, Matsumoto K, Toh e A. IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:757-68 pubmed..Our results suggest that the IRA1 protein inhibits the function of the RAS proteins in a fashion antagonistic to the function of the CDC25 protein in the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. ..
- Ohnishi M, Nakagawara K, Mori M, Kato S, Sasahara Y, Kusuda K, et al. Localization of the mouse protein serine/threonine phosphatase 2C beta gene to chromosome 17E 4-5. Genomics. 1996;32:134-6 pubmed..It has two isotypes, alpha and beta, encoded by different genes. In this study, the mouse PP2C beta gene was mapped by in situ hybridization to chromosome 17E 4-5. ..
- Okamoto M, Yoko o T, Umemura M, Nakayama K, Jigami Y. Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p. J Biol Chem. 2006;281:4013-23 pubmed
- Yazawa H, Iwahashi H, Uemura H. Disruption of URA7 and GAL6 improves the ethanol tolerance and fermentation capacity of Saccharomyces cerevisiae. Yeast. 2007;24:551-60 pubmed..When the cells were grown in medium containing 5% ethanol at 15 degrees C, the gal6 and ura7 disruptants showed 40% and 14% increases in the glucose consumption rate, respectively. ..
- Ogata T. Nitrogen starvation induces expression of Lg-FLO1 and flocculation in bottom-fermenting yeast. Yeast. 2012;29:487-94 pubmed publisher..This suggests that the flocculation of bottom-fermenting yeast is under the control of a nitrogen catabolite repression (NCR)-like mechanism. ..
- Yompakdee C, Bun Ya M, Shikata K, Ogawa N, Harashima S, Oshima Y. A putative new membrane protein, Pho86p, in the inorganic phosphate uptake system of Saccharomyces cerevisiae. Gene. 1996;171:41-7 pubmed
- Shin H, Shinotsuka C, Torii S, Murakami K, Nakayama K. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p. J Biochem. 1997;122:525-30 pubmed..These data suggest that DVLP is not involved in the formation of known coated vesicles. ..
- Matsuyama A, Yabana N, Watanabe Y, Yamamoto M. Schizosaccharomyces pombe Ste7p is required for both promotion and withholding of the entry to meiosis. Genetics. 2000;155:539-49 pubmed..These observations suggest that Ste7p may have a function to suppress the onset of meiosis until the conjugation process has been duly completed. ..
- Altaf Ul Amin M, Shinbo Y, Mihara K, Kurokawa K, Kanaya S. Development and implementation of an algorithm for detection of protein complexes in large interaction networks. BMC Bioinformatics. 2006;7:207 pubmed..Therefore, protein complexes determined solely based on interaction data can help us to predict the functions of proteins, and they are also useful to understand and explain certain biological processes. ..
- Horigome C, Ikeda R, Okada T, Takenami K, Mizuta K. Genetic interaction between ribosome biogenesis and inositol polyphosphate metabolism in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2009;73:443-6 pubmed..These results suggest that inositol polyphosphate metabolism affects ribosome biogenesis in yeast. ..
- Kubota H, Sakaki Y, Ito T. GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast. J Biol Chem. 2000;275:20243-6 pubmed..Thus, GI domain-mediated association of GCN2 to GCN1 is required for general amino acid control. This finding provides the first insight into the molecular mechanism for the activation of GCN2 by GCN1. ..
- Cui Z, Horecka J, Jigami Y. Cdc4 is involved in the transcriptional control of OCH1, a gene encoding alpha-1,6-mannosyltransferase in Saccharomyces cerevisiae. Yeast. 2002;19:69-77 pubmed..Interestingly, cdc4(bon) and Delta swi4 show a synthetic growth defect when combined. ..
- Miyake S, Yamamoto M. Identification of ras-related, YPT family genes in Schizosaccharomyces pombe. EMBO J. 1990;9:1417-22 pubmed..pombe Gly-17). The physiological roles of these genes appear to be distinct because each of them is indispensable for cell growth. ..
- Sato N, Arai K, Masai H. Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7. EMBO J. 1997;16:4340-51 pubmed..The wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions. ..
- Hiraishi H, Mochizuki M, Takagi H. Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes. Biosci Biotechnol Biochem. 2006;70:2762-5 pubmed..These results suggest that overexpression of ubiquitin-related enzymes might be a useful method for breeding novel stress-resistant strains. ..
- Odani T, Shimma Y, Tanaka A, Jigami Y. Cloning and analysis of the MNN4 gene required for phosphorylation of N-linked oligosaccharides in Saccharomyces cerevisiae. Glycobiology. 1996;6:805-10 pubmed..The results from genetic and biochemical experiments combine to suggest that Mnn4p is required to mediate mannosylphosphate transfer in both the core and outer chain portions of N-linked oligosaccharides. ..
- Kainuma M, Chiba Y, Takeuchi M, Jigami Y. Overexpression of HUT1 gene stimulates in vivo galactosylation by enhancing UDP-galactose transport activity in Saccharomyces cerevisiae. Yeast. 2001;18:533-41 pubmed..These results suggest a role for Hut1p in the transport of UDP--galactose from the cytosol into the Golgi lumen in S. cerevisiae. ..
- Toh e A, Oguchi T. Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae. Genes Genet Syst. 2001;76:393-410 pubmed..We propose that a defect in GPI anchor synthesis has multiple consequences, including activation of the Hog1 MAP kinase cascade and conferring copper-resistance. ..
- Hori O, Ichinoda F, Tamatani T, Yamaguchi A, Sato N, Ozawa K, et al. Transmission of cell stress from endoplasmic reticulum to mitochondria: enhanced expression of Lon protease. J Cell Biol. 2002;157:1151-60 pubmed
- Matsui Y, Matsui R, Akada R, Toh e A. Yeast src homology region 3 domain-binding proteins involved in bud formation. J Cell Biol. 1996;133:865-78 pubmed..Overproduction of either Rho3p or the Rho3p-related GTPase Rho4p suppressed the boi defect. These results demonstrate that Rho3p GTPases and Boi proteins function in the maintenance of cell polarity for bud formation. ..
- Suzuki C, Shimma Y. P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT. Mol Microbiol. 1999;32:813-23 pubmed..The glycosylation-defective phenotype and possible killer-resistant mechanisms are discussed in comparison with the Golgi Ca2+ pump Pmr1p...
- Mito Y, Sugimoto A, Yamamoto M. Distinct developmental function of two Caenorhabditis elegans homologs of the cohesin subunit Scc1/Rad21. Mol Biol Cell. 2003;14:2399-409 pubmed
- Shitamukai A, Hirata D, Sonobe S, Miyakawa T. Evidence for antagonistic regulation of cell growth by the calcineurin and high osmolarity glycerol pathways in Saccharomyces cerevisiae. J Biol Chem. 2004;279:3651-61 pubmed
- Mukai H, Shuntoh H, Chang C, Asami M, Ueno M, Suzuki K, et al. Isolation and characterization of CAJ1, a novel yeast homolog of dnaJ. Gene. 1994;145:125-7 pubmed..Although CAJ1 contains neither a Gly-rich region nor a Cys-rich repeat, as are found in other DnaJ relatives, it contains a leucine zipper-like motif. ..
- Kotani T, Miyake T, Tsukihashi Y, Hinnebusch A, Nakatani Y, Kawaichi M, et al. Identification of highly conserved amino-terminal segments of dTAFII230 and yTAFII145 that are functionally interchangeable for inhibiting TBP-DNA interactions in vitro and in promoting yeast cell growth in vivo. J Biol Chem. 1998;273:32254-64 pubmed
- Kimura A, Kato Y, Hirano H. N-myristoylation of the Rpt2 subunit regulates intracellular localization of the yeast 26S proteasome. Biochemistry. 2012;51:8856-66 pubmed publisher..Our results suggest that N-myristoylation is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome. ..
- Hisamoto N, Frederick D, Sugimoto K, Tatchell K, Matsumoto K. The EGP1 gene may be a positive regulator of protein phosphatase type 1 in the growth control of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3767-76 pubmed..Egp1 and PP1 directly interact, as assayed by coimmunoprecipitation. These results suggest that Egp1 functions as a positive modulator of PP1 in the growth control of S. cerevisiae. ..
- Araki H, Leem S, Phongdara A, Sugino A. Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A. 1995;92:11791-5 pubmed..These results strongly suggest that Dpb11 is a part of the DNA polymerase II complex during chromosomal DNA replication and also acts in a checkpoint pathway during the S phase of the cell cycle to sense stalled DNA replication. ..
- Miyahara K, Hirata D, Miyakawa T. yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae. Curr Genet. 1996;29:103-5 pubmed..A sequence similar to the yAP-1 recognition element recently identified in the stress-responsive yeast genes was found in this region and in the 5'-flanking sequences of SNQ2...
- Kametaka S, Matsuura A, Wada Y, Ohsumi Y. Structural and functional analyses of APG5, a gene involved in autophagy in yeast. Gene. 1996;178:139-43 pubmed
- Osakabe K, Yoshioka T, Ichikawa H, Toki S. Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana. Plant Mol Biol. 2002;50:71-81 pubmed..The results of yeast two-hybrid assays suggested that Arabidopsis Rad51 family proteins form a complex, which could participate in meiotic recombination and/or homologous recombinational repair. ..
- Toh e A, Oguchi T. Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst. 2002;77:309-22 pubmed..Las21 did not show preference for the metabolic pathway to produce phosphatidylethanolamine. ..
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