Experts and Doctors on saccharomyces cerevisiae proteins in Italy

Summary

Locale: Italy
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Gamberi C, Contreas G, Romanelli M, Morandi C. Analysis of the yeast NSR1 gene and protein domain comparison between Nsr1 and human hnRNP type A1. Gene. 1994;148:59-66 pubmed
    ..The biochemical data, therefore, would support the hypothesis that the two RRM domains in hnRNP A1 and Nsr1 interact with RNA in a similar manner in both mammalian and yeast cells, respectively. ..
  2. Lira M, Mottes M, Pignatti P, Medica I, Uziel G, Cappa M, et al. Detection of mutations in the ALD gene (ABCD1) in seven Italian families: description of four novel mutations. Hum Mutat. 2000;16:271 pubmed
    ..Mutations 2014C>T (P543L), 2053A>G (Q556A), 673-674insCC, and 1874+1G>A are described for the first time in this report. Mutations 1638C>T (R418W), 1588G>A(R401Q), and 1801-1802delAG are already known to be link to ALD...
  3. Paciotti V, Clerici M, Scotti M, Lucchini G, Longhese M. Characterization of mec1 kinase-deficient mutants and of new hypomorphic mec1 alleles impairing subsets of the DNA damage response pathway. Mol Cell Biol. 2001;21:3913-25 pubmed
  4. Verdone L, Wu J, Van Riper K, Kacherovsky N, Vogelauer M, Young E, et al. Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions. EMBO J. 2002;21:1101-11 pubmed
  5. Dusi C, Mistretta M, Cornaglia G, Fontana R. Interaction with CEP-1 beta-lactamase of RU 51746-2, the active form of the new oral cephalosporin RU 51807. J Chemother. 1991;3 Suppl 1:54-6 pubmed
    ..This suggests that the slow hydrolysis of RU 51746-2 has no clinical meaning, since the activity of this drug is virtually unaffected by the amount of beta-lactamases usually found in wild strains. ..
  6. Crespan E, Zanoli S, Khandazhinskaya A, Shevelev I, Jasko M, Alexandrova L, et al. Incorporation of non-nucleoside triphosphate analogues opposite to an abasic site by human DNA polymerases beta and lambda. Nucleic Acids Res. 2005;33:4117-27 pubmed
    ..These results show for the first time that neither the base nor the sugar moieties of nucleotides are required for incorporation by family X DNA polymerases. ..
  7. D Angelo G, Vicinanza M, Di Campli A, De Matteis M. The multiple roles of PtdIns(4)P -- not just the precursor of PtdIns(4,5)P2. J Cell Sci. 2008;121:1955-63 pubmed publisher
    ..The aim of this Commentary is to describe our present knowledge of PtdIns(4)P metabolism and the molecular machineries that are directly regulated by PtdIns(4)P within and outside of the Golgi complex. ..
  8. Fachinetti D, Bermejo R, Cocito A, Minardi S, Katou Y, Kanoh Y, et al. Replication termination at eukaryotic chromosomes is mediated by Top2 and occurs at genomic loci containing pausing elements. Mol Cell. 2010;39:595-605 pubmed publisher
    ..We propose that in eukaryotes, replication fork barriers, Rrm3, and Top2 coordinate replication fork progression and fusion at TERs, thus counteracting abnormal genomic transitions. ..
  9. Gobbini E, Cesena D, Galbiati A, Lockhart A, Longhese M. Interplays between ATM/Tel1 and ATR/Mec1 in sensing and signaling DNA double-strand breaks. DNA Repair (Amst). 2013;12:791-9 pubmed publisher
    ..Here, we review the early steps of DSB processing and the role of DNA-end structures in activating ATM/Tel1 and ATR/Mec1 in an orderly and reciprocal manner. ..

More Information

Publications122 found, 100 shown here

  1. Licursi V, Salvi C, De Cesare V, Rinaldi T, Mattei B, Fabbri C, et al. The COP9 signalosome is involved in the regulation of lipid metabolism and of transition metals uptake in Saccharomyces cerevisiae. FEBS J. 2014;281:175-90 pubmed publisher
    ..Our results suggest that the diverged budding yeast CSN is more conserved than was previously thought. ..
  2. Palumbo P, Vanoni M, Cusimano V, Busti S, Marano F, Manes C, et al. Whi5 phosphorylation embedded in the G1/S network dynamically controls critical cell size and cell fate. Nat Commun. 2016;7:11372 pubmed publisher
  3. Cartagena Lirola H, Guerini I, Manfrini N, Lucchini G, Longhese M. Role of the Saccharomyces cerevisiae Rad53 checkpoint kinase in signaling double-strand breaks during the meiotic cell cycle. Mol Cell Biol. 2008;28:4480-93 pubmed publisher
  4. Amata I, Gallo M, Pennestri M, Paci M, Ragnini Wilson A, Cicero D. N-lobe dynamics of myosin light chain dictates its mode of interaction with myosin V IQ1. Biochemistry. 2008;47:12332-45 pubmed publisher
    ..These data show that the core characteristics of myosin light chain N-lobes differentiate Mlc1p and calmodulin binding capability. ..
  5. Pagani M, Pilati S, Bertoli G, Valsasina B, Sitia R. The C-terminal domain of yeast Ero1p mediates membrane localization and is essential for function. FEBS Lett. 2001;508:117-20 pubmed
    ..Appending the yeast C-terminal tail to human Ero1-Lalpha restores membrane association and allows growth of ERO1 disrupted cells. Therefore, the tail of Ero1p mediates membrane association and is crucial for function. ..
  6. Alberghina L, Rossi R, Querin L, Wanke V, Vanoni M. A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast. J Cell Biol. 2004;167:433-43 pubmed
    ..We show that a second threshold is required together with the Cln3/Far1 threshold for carbon source modulation of Ps. A new molecular network accounting for the setting of Ps is proposed. ..
  7. Mazzoni C, Palermo V, Torella M, Falcone C. HIR1, the co-repressor of histone gene transcription of Saccharomyces cerevisiae, acts as a multicopy suppressor of the apoptotic phenotypes of the LSM4 mRNA degradation mutant. FEMS Yeast Res. 2005;5:1229-35 pubmed
    ..Transcription analysis revealed that the expression of histone genes was lowered in the mutant over-expressing HIR1, indicating a relationship between the latter gene and apoptosis. ..
  8. Martina M, Clerici M, Baldo V, Bonetti D, Lucchini G, Longhese M. A balance between Tel1 and Rif2 activities regulates nucleolytic processing and elongation at telomeres. Mol Cell Biol. 2012;32:1604-17 pubmed publisher
    ..Altogether, these findings highlight a primary role of Tel1 in overcoming Rif2-dependent negative regulation of MRX activity in telomere resection and elongation. ..
  9. Busnelli S, Tripodi F, Nicastro R, Cirulli C, Tedeschi G, Pagliarin R, et al. Snf1/AMPK promotes SBF and MBF-dependent transcription in budding yeast. Biochim Biophys Acta. 2013;1833:3254-3264 pubmed publisher
  10. Turchini A, Ferrario L, Popolo L. Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae. J Bacteriol. 2000;182:1167-71 pubmed
    ..GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway. ..
  11. Lottersberger F, Panza A, Lucchini G, Piatti S, Longhese M. The Saccharomyces cerevisiae 14-3-3 proteins are required for the G1/S transition, actin cytoskeleton organization and cell wall integrity. Genetics. 2006;173:661-75 pubmed
    ..Remarkably, budding and DNA replication defects of bmh mutants were suppressed by CLN2 expression from an SBF-independent promoter, suggesting that 14-3-3 proteins might contribute to regulating the late G(1) transcriptional program. ..
  12. Palumbo M, Colosimo A, Giuliani A, Farina L. Essentiality is an emergent property of metabolic network wiring. FEBS Lett. 2007;581:2485-9 pubmed
    ..We demonstrate that the 'missing alternative' paradigm is sufficient to explain the generation of essentiality for double mutations in which each single deleted element is non-essential. ..
  13. Groppi S, Belotti F, Brandão R, Martegani E, Tisi R. Glucose-induced calcium influx in budding yeast involves a novel calcium transport system and can activate calcineurin. Cell Calcium. 2011;49:376-86 pubmed publisher
  14. De Vendittis E, Fasano O. Energetic aspects of intramolecular coupling between the nucleotide binding site and the distal switch II region of the yeast RAS2 protein. FEBS Lett. 1994;347:133-6 pubmed
  15. Contini C, Cultrera R, Seraceni S, Segala D, Romani R, Fainardi E, et al. The role of stage-specific oligonucleotide primers in providing effective laboratory support for the molecular diagnosis of reactivated Toxoplasma gondii encephalitis in patients with AIDS. J Med Microbiol. 2002;51:879-90 pubmed
  16. Paoli P, Modesti A, Magherini F, Gamberi T, Caselli A, Manao G, et al. Site-directed mutagenesis of two aromatic residues lining the active site pocket of the yeast Ltp1. Biochim Biophys Acta. 2007;1770:753-62 pubmed
  17. Doksani Y, Bermejo R, Fiorani S, Haber J, Foiani M. Replicon dynamics, dormant origin firing, and terminal fork integrity after double-strand break formation. Cell. 2009;137:247-58 pubmed publisher
    ..Our findings have implications for those genome instability syndromes that accumulate DNA breaks during S phase and for forks encountering eroding telomeres. ..
  18. Vecchi M, Polo S, Poupon V, Van de Loo J, Benmerah A, Di Fiore P. Nucleocytoplasmic shuttling of endocytic proteins. J Cell Biol. 2001;153:1511-7 pubmed
  19. Brocca S, Samalikova M, Uversky V, Lotti M, Vanoni M, Alberghina L, et al. Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1. Proteins. 2009;76:731-46 pubmed publisher
    ..The mutations S201A and S201E, which are known to affect Sic1 function, do not have significant effects on the conformational properties of the pure protein. ..
  20. Saponaro M, Callahan D, Zheng X, Krejci L, Haber J, Klein H, et al. Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote recombinational repair. PLoS Genet. 2010;6:e1000858 pubmed publisher
    ..We suggest that Cdk1 kinase counteracts unscheduled sumoylation of Srs2 and targets Srs2 to dismantle specific DNA structures, such as the D-loops, in a helicase-dependent manner during homologous recombinational repair. ..
  21. Martegani E, Vanoni M, Zippel R, Coccetti P, Brambilla R, Ferrari C, et al. Cloning by functional complementation of a mouse cDNA encoding a homologue of CDC25, a Saccharomyces cerevisiae RAS activator. EMBO J. 1992;11:2151-7 pubmed
    ..Northern blot analysis of mouse brain poly(A)+ RNA reveals two major transcripts of approximately 1700 and 5200 nucleotides. Transcripts were found also in mouse heart and at a lower level in liver and spleen. ..
  22. Clerici M, Paciotti V, Baldo V, Romano M, Lucchini G, Longhese M. Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression. EMBO J. 2001;20:6485-98 pubmed
    ..In addition, Tel1 overproduction results in transient nuclear division arrest and concomitant Rad53 phosphorylation in the absence of exogenous DNA damage independently of Mec1 and Ddc1. ..
  23. Zara V, Ferramosca A, Papatheodorou P, Palmieri F, Rassow J. Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation. J Cell Sci. 2005;118:3985-95 pubmed
    ..We conclude that presequences can only act as mediators of mitochondrial protein import if they allow rapid release from import receptor sites. Release from receptors sites may be rate-limiting in translocation. ..
  24. Amigoni L, Frigerio G, Martegani E, Colombo S. Involvement of Aif1 in apoptosis triggered by lack of Hxk2 in the yeast Saccharomyces cerevisiae. FEMS Yeast Res. 2016;16: pubmed publisher
    ..Moreover, we show that active Ras proteins relocalize to the plasma membrane and to the nucleus in hxk2Δ aif1Δ cells. ..
  25. Branzei D, Foiani M. The Rad53 signal transduction pathway: Replication fork stabilization, DNA repair, and adaptation. Exp Cell Res. 2006;312:2654-9 pubmed
  26. Mazzoni C, Mancini P, Verdone L, Madeo F, Serafini A, Herker E, et al. A truncated form of KlLsm4p and the absence of factors involved in mRNA decapping trigger apoptosis in yeast. Mol Biol Cell. 2003;14:721-9 pubmed
    ..This is the first time that a connection between mRNA stability and apoptosis is reported in yeast, pointing to mRNA decapping as the crucial step responsible of the observed apoptotic phenotypes. ..
  27. Ang S, Zhang M, Lodi T, Lu H. Mitochondrial thiol oxidase Erv1: both shuttle cysteine residues are required for its function with distinct roles. Biochem J. 2014;460:199-210 pubmed publisher
    ..Taken together, we conclude that both shuttle cysteine residues are required for Erv1 function, and play complementary, but distinct, roles to ensure rapid turnover of active Erv1. ..
  28. Alzu A, Bermejo R, Begnis M, Lucca C, Piccini D, Carotenuto W, et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell. 2012;151:835-46 pubmed publisher
  29. Baruffini E, Lodi T, Dallabona C, Puglisi A, Zeviani M, Ferrero I. Genetic and chemical rescue of the Saccharomyces cerevisiae phenotype induced by mitochondrial DNA polymerase mutations associated with progressive external ophthalmoplegia in humans. Hum Mol Genet. 2006;15:2846-55 pubmed
    ..Therefore, an increase of the mitochondrial dNTP pool and/or a decrease of reactive oxygen species can prevent the mtDNA damage induced by pol gamma mutations in yeast and, possibly, in humans. ..
  30. Marchetta M, Gamberi T, Sarno S, Magherini F, Raugei G, Camici G, et al. Expression of the Stp1 LMW-PTP and inhibition of protein CK2 display a cooperative effect on immunophilin Fpr3 tyrosine phosphorylation and Saccharomyces cerevisiae growth. Cell Mol Life Sci. 2004;61:1176-84 pubmed
    ..These data disclose a functional correlation between CK2 and LMW-PTPs, and suggest that reversible phosphorylation of Fpr3 plays a role in the regulation of growth rate and budding in S. cerevisiae. ..
  31. Rancati G, Crispo V, Lucchini G, Piatti S. Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast. Cell Cycle. 2005;4:972-80 pubmed
    ..Accordingly, replacing with alanines five serine residues belonging to Polo kinase-dependent putative phosphorylation sites dramatically reduces Mad3 phosphorylation, suggesting that Mad3 is likely an in vivo target of Cdc5. ..
  32. Ugolini S, Tosato V, Bruschi C. Selective fitness of four episomal shuttle-vectors carrying HIS3, LEU2, TRP1, and URA3 selectable markers in Saccharomyces cerevisiae. Plasmid. 2002;47:94-107 pubmed
    ..A potential correlation of the energy cost of plasmid maintenance with the secondary DNA structure and the level of expression of the selective markers is also investigated. ..
  33. Clerici M, Mantiero D, Guerini I, Lucchini G, Longhese M. The Yku70-Yku80 complex contributes to regulate double-strand break processing and checkpoint activation during the cell cycle. EMBO Rep. 2008;9:810-8 pubmed publisher
    ..Moreover, DSB resection in ykuDelta cells occurs independently of CDK activity, suggesting that it might be promoted by CDK-dependent inhibition of Yku. ..
  34. Grassi L, Tramontano A. Horizontal and vertical growth of S. cerevisiae metabolic network. BMC Evol Biol. 2011;11:301 pubmed publisher
    ..cerevisiae metabolic network. Interestingly, the effects of a patchwork strategy acting before the Euascomycete-Hemiascomycete divergence are still detectable today. ..
  35. Atorino L, Silvestri L, Koppen M, Cassina L, Ballabio A, Marconi R, et al. Loss of m-AAA protease in mitochondria causes complex I deficiency and increased sensitivity to oxidative stress in hereditary spastic paraplegia. J Cell Biol. 2003;163:777-87 pubmed
    ..These results shed new light on the molecular pathogenesis of HSP and functionally link AFG3L2 to this neurodegenerative disease. ..
  36. Masciadri B, Areces L, Carpinelli P, Foiani M, Draetta G, Fiore F. Characterization of the BUD31 gene of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2004;320:1342-50 pubmed
    ..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs. ..
  37. Mantiero D, Clerici M, Lucchini G, Longhese M. Dual role for Saccharomyces cerevisiae Tel1 in the checkpoint response to double-strand breaks. EMBO Rep. 2007;8:380-7 pubmed
    ..Furthermore, we provide evidence that the kinetics of DSB resection can influence Tel1 activation, indicating that processing of the DSB termini might influence the transition from Tel1/ATM- to Mec1/ATR-dependent checkpoint. ..
  38. Longhese M, Fraschini R, Plevani P, Lucchini G. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase. Mol Cell Biol. 1996;16:3235-44 pubmed
  39. Gonzalez Huici V, Szakal B, Urulangodi M, Psakhye I, Castellucci F, Menolfi D, et al. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. EMBO J. 2014;33:327-40 pubmed publisher
    ..Together, the results suggest that replication-associated topological changes involving the molecular DNA bender, Hmo1, set the stage for dedicated repair reactions that limit errors during replication and impact on genome stability. ..
  40. Semplici F, Meggio F, Pinna L, Oliviero S. CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Oncogene. 2002;21:3978-87 pubmed
    ..Taken together these data suggest that CK2-dependent phosphorylation of UBC3 and UBC3B functions by regulating beta-TrCP substrate recognition. ..
  41. Branzei D, Sollier J, Liberi G, Zhao X, Maeda D, Seki M, et al. Ubc9- and mms21-mediated sumoylation counteracts recombinogenic events at damaged replication forks. Cell. 2006;127:509-22 pubmed
    ..Our results indicate that Ubc9- and Mms21-mediated sumoylation functions as a regulatory mechanism, different from that of replication checkpoints, to prevent pathological accumulation of cruciform structures at damaged forks. ..
  42. Simonetta M, Manzoni R, Mosca R, Mapelli M, Massimiliano L, Vink M, et al. The influence of catalysis on mad2 activation dynamics. PLoS Biol. 2009;7:e10 pubmed publisher
    ..e., it is purely catalytic. These results surpass previously formulated objections to the Mad2-template model and predict that the release of Mad2 from Cdc20 is an energy-driven process. ..
  43. Pagliuca C, Draviam V, Marco E, Sorger P, De Wulf P. Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. PLoS ONE. 2009;4:e7640 pubmed publisher
    ..The failure of Spc105 deficient kinetochores to bind correctly to spindle microtubules and to recruit checkpoint proteins in yeast and human cells explains the observed severity of missegregation phenotypes. ..
  44. Donzelli M, Squatrito M, Ganoth D, Hershko A, Pagano M, Draetta G. Dual mode of degradation of Cdc25 A phosphatase. EMBO J. 2002;21:4875-84 pubmed
    ..We propose that a dual mechanism of regulated degradation allows for fine tuning of Cdc25 A abundance in response to cell environment. ..
  45. Chiolo I, Saponaro M, Baryshnikova A, Kim J, Seo Y, Liberi G. The human F-Box DNA helicase FBH1 faces Saccharomyces cerevisiae Srs2 and postreplication repair pathway roles. Mol Cell Biol. 2007;27:7439-50 pubmed
    ..Overall, our findings suggest that the hFBH1 helicase is a functional human orthologue of budding yeast Srs2 that also possesses self-regulation properties necessary to execute its recombination functions. ..
  46. Puddu F, Granata M, di Nola L, Balestrini A, Piergiovanni G, Lazzaro F, et al. Phosphorylation of the budding yeast 9-1-1 complex is required for Dpb11 function in the full activation of the UV-induced DNA damage checkpoint. Mol Cell Biol. 2008;28:4782-93 pubmed publisher
    ..Finally, we show that, in vivo, Dpb11 cooperates with Dot1 in promoting Rad9 phosphorylation but also contributes to the full activation of Mec1 kinase. ..
  47. Nardi T, Corich V, Giacomini A, Blondin B. A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast. Microbiology. 2010;156:1686-96 pubmed publisher
    ..The lack of differences between promoter regions suggests that this inducible SSU1 expression pattern is due to modification of regulatory/signalling pathways. ..
  48. Bermejo R, Doksani Y, Capra T, Katou Y, Tanaka H, Shirahige K, et al. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation. Genes Dev. 2007;21:1921-36 pubmed
    ..A failure in resolving fork-related topological constrains during S phase may therefore result in abnormal chromosome transitions, DNA damage checkpoint activation, and chromosome breakage during segregation. ..
  49. Nikitin D, Tosato V, Zavec A, Bruschi C. Cellular and molecular effects of nonreciprocal chromosome translocations in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2008;105:9703-8 pubmed publisher
    ..These BIT translocants could serve as model systems to understand further the cellular and molecular effects of chromosome translocation and provide fundamental information on its etiology of neoplastic transformation in mammals. ..
  50. Taramino S, Teske B, Oliaro Bosso S, Bard M, Balliano G. Divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts. Biochim Biophys Acta. 2010;1801:1232-7 pubmed publisher
    ..Results demonstrate that in mammals, unlike in yeast, OSC and steroid-3-ketoreductase are non-interacting proteins. ..
  51. Palmieri L, Alberio S, Pisano I, Lodi T, Meznaric Petrusa M, Zidar J, et al. Complete loss-of-function of the heart/muscle-specific adenine nucleotide translocator is associated with mitochondrial myopathy and cardiomyopathy. Hum Mol Genet. 2005;14:3079-88 pubmed
  52. Foiani M, Nadjar Boger E, Capone R, Sagee S, Hashimshoni T, Kassir Y. A meiosis-specific protein kinase, Ime2, is required for the correct timing of DNA replication and for spore formation in yeast meiosis. Mol Gen Genet. 1996;253:278-88 pubmed
    ..In addition, we show that Ime2 is not absolutely required for commitment to meiotic recombination, spindle formation and nuclear division, although it is required for spore formation. ..
  53. Montecucco A, Rossi R, Levin D, Gary R, Park M, Motycka T, et al. DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories. EMBO J. 1998;17:3786-95 pubmed
  54. Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, et al. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999;8:1397-407 pubmed
    ..Together, these data suggest that midin and MID2 have a similar biochemical function but a different physiological role during development. ..
  55. Fazi B, Cope M, Douangamath A, Ferracuti S, Schirwitz K, Zucconi A, et al. Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis. J Biol Chem. 2002;277:5290-8 pubmed
    ..We have shown by site-directed mutagenesis that one of these negatively charged side chains may be the key determinant for the preference for non-classical ligands. ..
  56. Giannattasio M, Sommariva E, Vercillo R, Lippi Boncambi F, Liberi G, Foiani M, et al. A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1. Proc Natl Acad Sci U S A. 2002;99:12997-3002 pubmed
    ..This work thus reports a previously undiscovered role for Tel1 in checkpoint control. ..
  57. Saliner A, Netzeva T, Worth A. Prediction of estrogenicity: validation of a classification model. SAR QSAR Environ Res. 2006;17:195-223 pubmed
    ..In addition, the model was shown to meet the OECD Principles for (Q)SAR Validation, making it potentially useful for regulatory purposes. ..
  58. Bailly Bechet M, Braunstein A, Pagnani A, Weigt M, Zecchina R. Inference of sparse combinatorial-control networks from gene-expression data: a message passing approach. BMC Bioinformatics. 2010;11:355 pubmed publisher
  59. Trotta E. Selection on codon bias in yeast: a transcriptional hypothesis. Nucleic Acids Res. 2013;41:9382-95 pubmed publisher
    ..The results reported here are consistent with a role for transcriptional forces in driving codon usage bias via a mechanism that improves gene expression by optimizing mRNA folding structures. ..
  60. Ognibene M, Vanni C, Blengio F, Segalerba D, Mancini P, De Marco P, et al. Identification of a novel mouse Dbl proto-oncogene splice variant: evidence that SEC14 domain is involved in GEF activity regulation. Gene. 2014;537:220-9 pubmed publisher
    ..We show here that an altered SEC14 sequence leads to enhanced Dbl translocation to the plasma membrane and to augmented transforming and exchange activity. ..
  61. Modesti A, Cirri P, Raugei G, Carraresi L, Magherini F, Manao G, et al. Expression, purification and kinetic behaviour of fission yeast low M(r) protein-tyrosine phosphatase. FEBS Lett. 1995;375:235-8 pubmed
    ..These differing kinetic characteristics are mainly due to the sequence 45-56 that is spatially close to the active site pocket. ..
  62. Marini F, Pellicioli A, Paciotti V, Lucchini G, Plevani P, Stern D, et al. A role for DNA primase in coupling DNA replication to DNA damage response. EMBO J. 1997;16:639-50 pubmed
    ..Altogether, our results suggest that DNA primase plays an essential role in a subset of the Rad53p-dependent checkpoint pathways controlling cell cycle progression in response to DNA damage. ..
  63. Martegani E, Vanoni M, Mauri I, Rudoni S, Saliola M, Alberghina L. Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae. Yeast. 1997;13:391-7 pubmed
    ..cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption. ..
  64. Vigano M, Di Rocco G, Zappavigna V, Mavilio F. Definition of the transcriptional activation domains of three human HOX proteins depends on the DNA-binding context. Mol Cell Biol. 1998;18:6201-12 pubmed
  65. Sironi L, Melixetian M, Faretta M, Prosperini E, Helin K, Musacchio A. Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. EMBO J. 2001;20:6371-82 pubmed
    ..We propose a model that features the kinetochore as a 'folding factory' for the formation of a Mad2-Cdc20 complex endowed with inhibitory activity on the anaphase promoting complex. ..
  66. Rinaldi T, Gambadoro A, Francisci S, Frontali L. Nucleo-mitochondrial interactions in Saccharomyces cerevisiae: characterization of a nuclear gene suppressing a defect in mitochondrial tRNA(Asp) processing. Gene. 2003;303:63-8 pubmed
    ..The gene contains a domain highly conserved in evolution from bacteria to human and its product has been recently shown to have dihydrouridine synthase activity. ..
  67. Robert T, Vanoli F, Chiolo I, Shubassi G, Bernstein K, Rothstein R, et al. HDACs link the DNA damage response, processing of double-strand breaks and autophagy. Nature. 2011;471:74-79 pubmed publisher
    ..We propose that Rpd3, Hda1 and Gcn5 control chromosome stability by coordinating the ATR checkpoint and double-strand-break processing with autophagy. ..
  68. Manfrini N, Gobbini E, Baldo V, Trovesi C, Lucchini G, Longhese M. G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint. Mol Cell Biol. 2012;32:4971-85 pubmed publisher
    ..Moreover, these findings suggest that the essential function of Mec1 and Rad53 is not necessarily separated from the function of these kinases in supporting DNA synthesis under stress conditions. ..
  69. Tripodi F, Nicastro R, Busnelli S, Cirulli C, Maffioli E, Tedeschi G, et al. Protein kinase CK2 holoenzyme promotes start-specific transcription in Saccharomyces cerevisiae. Eukaryot Cell. 2013;12:1271-80 pubmed publisher
    ..Collectively, these findings suggest a novel role for the CK2 holoenzyme in the activation of G1 transcription. ..
  70. Meroni G, Reymond A, Alcalay M, Borsani G, Tanigami A, Tonlorenzi R, et al. Rox, a novel bHLHZip protein expressed in quiescent cells that heterodimerizes with Max, binds a non-canonical E box and acts as a transcriptional repressor. EMBO J. 1997;16:2892-906 pubmed
    ..3 in a region that frequently undergoes loss of heterozygosity in a number of malignancies, together with the biochemical and expression features, suggest involvement of ROX in human neoplasia. ..
  71. Stocchetto S, Marin O, Carignani G, Pinna L. Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. FEBS Lett. 1997;414:171-5 pubmed
    ..Mn2+ or, less effectively, Co2+ are required for piD261 catalytic activity, which is conversely undetectable in the presence of Mg2+, a behaviour unique among Ser/Thr protein kinases. ..
  72. Puri P, Sartorelli V, Yang X, Hamamori Y, Ogryzko V, Howard B, et al. Differential roles of p300 and PCAF acetyltransferases in muscle differentiation. Mol Cell. 1997;1:35-45 pubmed
    ..These results indicate that recruitment of histone acetyltransferase activity of PCAF by MyoD, through p300/CBP, is crucial for activation of the myogenic program. ..
  73. Babbio F, Farinacci M, Saracino F, Carbone M, Privitera E. Expression and localization studies of hSDA, the human ortholog of the yeast SDA1 gene. Cell Cycle. 2004;3:486-90 pubmed
    ..However our preliminary results indicate that hSDA does not behave like a proapoptotic gene and its involvement in tumorigenesis is still to be clarified. ..
  74. Bianchi M, Costanzo G, Chelstowska A, Grabowska D, Mazzoni C, Piccinni E, et al. The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast. Mol Microbiol. 2004;53:953-68 pubmed
  75. Belotti F, Tisi R, Martegani E. The N-terminal region of the Saccharomyces cerevisiae RasGEF Cdc25 is required for nutrient-dependent cell-size regulation. Microbiology. 2006;152:1231-42 pubmed
    ..Further work will aim to clarify the role of this region in Cdc25 activity and Ras/cAMP pathway regulation. ..
  76. Guaragnella N, Pereira C, Sousa M, Antonacci L, Passarella S, Corte Real M, et al. YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase-like activity. FEBS Lett. 2006;580:6880-4 pubmed
    ..Since in Deltayca1 cells this effect is lost, but z-VAD-fmk does not prevent both WT and Deltayca1 cell death, PCD in WT cells occurs via a Yca1p caspase and a non-caspase route with similar characteristics. ..
  77. Paiardi C, Belotti F, Colombo S, Tisi R, Martegani E. The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation. FEMS Yeast Res. 2007;7:1270-5 pubmed
  78. Gazzarrini S, Kang M, Abenavoli A, Romani G, Olivari C, Gaslini D, et al. Chlorella virus ATCV-1 encodes a functional potassium channel of 82 amino acids. Biochem J. 2009;420:295-303 pubmed publisher
    ..Hence, ATCV-1 Kcv is the smallest protein to form a K+ channel and it will serve as a model for studying structure-function correlations inside the potassium channel pore...
  79. Bazzi M, Mantiero D, Trovesi C, Lucchini G, Longhese M. Dephosphorylation of gamma H2A by Glc7/protein phosphatase 1 promotes recovery from inhibition of DNA replication. Mol Cell Biol. 2010;30:131-45 pubmed publisher
    ..We therefore propose that Glc7 activity promotes recovery from replication fork stalling caused by dNTP depletion and that gamma H2A dephosphorylation is a critical Glc7 function in this process. ..
  80. Manfrini N, Guerini I, Citterio A, Lucchini G, Longhese M. Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem. 2010;285:11628-37 pubmed publisher
    ..Furthermore, the helicase Sgs1 and the nucleases Exo1 and Dna2 participate in lengthening the 5'-3' resection tracts during meiosis by controlling a step subsequent to Spo11 removal. ..
  81. Manzoni R, Montani F, Visintin C, Caudron F, Ciliberto A, Visintin R. Oscillations in Cdc14 release and sequestration reveal a circuit underlying mitotic exit. J Cell Biol. 2010;190:209-22 pubmed publisher
    ..A common theme emerges where events that must happen only once per cycle, although intrinsically capable of oscillations, are limited to one occurrence by the cyclin-Cdk cell cycle engine. ..
  82. Sinibaldi F, Droghetti E, Polticelli F, Piro M, Di Pierro D, Ferri T, et al. The effects of ATP and sodium chloride on the cytochrome c-cardiolipin interaction: the contrasting behavior of the horse heart and yeast proteins. J Inorg Biochem. 2011;105:1365-72 pubmed publisher
  83. Magrì A, Di Rosa M, Tomasello M, Guarino F, Reina S, Messina A, et al. Overexpression of human SOD1 in VDAC1-less yeast restores mitochondrial functionality modulating beta-barrel outer membrane protein genes. Biochim Biophys Acta. 2016;1857:789-98 pubmed publisher
    ..Our results suggest a direct influence of SOD1 on VDAC. ..
  84. Cesena D, Cassani C, Rizzo E, Lisby M, Bonetti D, Longhese M. Regulation of telomere metabolism by the RNA processing protein Xrn1. Nucleic Acids Res. 2017;45:3860-3874 pubmed publisher
    ..These findings reveal novel roles for RNA processing proteins in the regulation of telomere metabolism with implications for genome stability in eukaryotes. ..
  85. Ferrari E, Bruhn C, Peretti M, Cassani C, Carotenuto W, Elgendy M, et al. PP2A Controls Genome Integrity by Integrating Nutrient-Sensing and Metabolic Pathways with the DNA Damage Response. Mol Cell. 2017;67:266-281.e4 pubmed publisher
    ..Our observations imply that metabolic changes affect genome integrity and may help with exploiting therapeutic options and repositioning known drugs. ..
  86. Santocanale C, Neecke H, Longhese M, Lucchini G, Plevani P. Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression. J Mol Biol. 1995;254:595-607 pubmed
    ..Finally, rfa2 mutant cells have a mutator and hyper-recombination phenotype and are more sensitive to hydroxyurea and methyl-methane-sulfonate than wild-type cells. ..
  87. Hateboer G, Wobst A, Petersen B, Le Cam L, Vigo E, Sardet C, et al. Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F. Mol Cell Biol. 1998;18:6679-97 pubmed
    ..In conclusion, our results provide a direct link between regulated progression through G1 controlled by the pRB pathway and the expression of proteins essential for the initiation of DNA replication. ..
  88. Majello B, Napolitano G, Lania L. Recruitment of the TATA-binding protein to the HIV-1 promoter is a limiting step for Tat transactivation. AIDS. 1998;12:1957-64 pubmed
    ..Thus, efficient recruitment of TBP represents a limiting step for Tat transactivation. ..
  89. Kuhne C, Banks L. E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box. J Biol Chem. 1998;273:34302-9 pubmed
  90. Longhese M, Paciotti V, Neecke H, Lucchini G. Checkpoint proteins influence telomeric silencing and length maintenance in budding yeast. Genetics. 2000;155:1577-91 pubmed
  91. Zara V, Palmisano I, Conte L, Trumpower B. Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b. Eur J Biochem. 2004;271:1209-18 pubmed
    ..Likewise, evidence of interactions between subunit 6, subunit 9 and cytochrome c1 suggests that a subcomplex between these two supernumerary subunits and the cytochrome might exist. ..
  92. Saracino F, Bassler J, Muzzini D, Hurt E, Agostoni Carbone M. The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects. Cell Cycle. 2004;3:648-54 pubmed
    ..Therefore we propose that Swe1, which is required for coordination of cell growth and cell division in G2/M, also has a role in the beginning of the cell cycle. ..