Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Germany
Topic: saccharomyces cerevisiae proteins
Publications439 found, 100 shown here
- Sikor M, Mapa K, von Voithenberg L, Mokranjac D, Lamb D. Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET. EMBO J. 2013;32:1639-49 pubmed publisher..These results indicate that the conformational cycle of Ssc1 is more elaborate than previously thought and provide insight into how the Hsp70s can perform a wide variety of functions. ..
- Huber E, Heinemeyer W, Li X, Arendt C, Hochstrasser M, Groll M. A unified mechanism for proteolysis and autocatalytic activation in the 20S proteasome. Nat Commun. 2016;7:10900 pubmed publisher..This work provides insights into the basic mechanism of proteolysis and propeptide autolysis, as well as the evolutionary pressures that drove the proteasome to become a threonine protease. ..
- Sanders G, Lichte B, Meyer H, Kilimann M. cDNA encoding the chicken ortholog of the mouse dilute gene product. Sequence comparison reveals a myosin I subfamily with conserved C-terminal domains. FEBS Lett. 1992;311:295-8 pubmed..Alternatively, this closely related clone might represent an isoform of the dilute protein derived from a second gene, potentially involved in genetic conditions related to dilute. ..
- van Dyck L, Neupert W, Langer T. The ATP-dependent PIM1 protease is required for the expression of intron-containing genes in mitochondria. Genes Dev. 1998;12:1515-24 pubmed..Transcripts of COXI and COB genes harboring multiple introns are degraded in the absence of PIM1. These results establish multiple, essential functions of the ATP-dependent PIM1 protease during mitochondrial gene expression. ..
- Wieland G, Hemmerich P, Koch M, Stoyan T, Hegemann J, Diekmann S. Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res. 2001;29:1054-60 pubmed..Our results suggest that centromeric DNA outside the consensus CDEI sequence and interaction of Cbf1p with adjacent centromere proteins contribute to the complex formation between Cbf1p and CEN DNA. ..
- Hoja U, Marthol S, Hofmann J, Stegner S, Schulz R, Meier S, et al. HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae. J Biol Chem. 2004;279:21779-86 pubmed..From this finding, it is concluded that HFA1 encodes a specific mitochondrial acetyl-CoA carboxylase providing malonyl-CoA for intraorganellar fatty acid and, in particular, lipoic acid synthesis. ..
- Meiling Wesse K, Barth H, Voss C, Eskelinen E, Epple U, Thumm M. Atg21 is required for effective recruitment of Atg8 to the preautophagosomal structure during the Cvt pathway. J Biol Chem. 2004;279:37741-50 pubmed
- Kambacheld M, Augustin S, Tatsuta T, Muller S, Langer T. Role of the novel metallopeptidase Mop112 and saccharolysin for the complete degradation of proteins residing in different subcompartments of mitochondria. J Biol Chem. 2005;280:20132-9 pubmed..These results suggest that the turnover of mitochondrial proteins is mediated by the sequential action of ATP-dependent proteases and oligopeptidases, some of them localized in the intermembrane space. ..
- Kail M, Hollinshead M, Kaufmann M, Boettcher J, Vaux D, Barnekow A. Yeast Ypt11 is targeted to recycling endosomes in mammalian cells. Biol Cell. 2005;97:651-8 pubmed
- Tüncher A, Spröte P, Gehrke A, Brakhage A. The CCAAT-binding complex of eukaryotes: evolution of a second NLS in the HapB subunit of the filamentous fungus Aspergillus nidulans despite functional conservation at the molecular level between yeast, A.nidulans and human. J Mol Biol. 2005;352:517-33 pubmed..nidulans hapB mutant was complemented, at least in part, by both the human NF-YA and S.cerevisiae Hap2p this finding suggests that the piggy-back mechanism of nuclear transport found for A.nidulans is conserved in yeast and human. ..
- Dragovic Z, Shomura Y, Tzvetkov N, Hartl F, Bracher A. Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p. Biol Chem. 2006;387:1593-600 pubmed..Interestingly, Fes1p inhibits the stimulation of Ssb1p ATPase by RAC, suggesting a complex regulatory role of Fes1p in modulating the function of Ssb Hsp70s in co-translational protein folding. ..
- Schladebeck S, Mösch H. The RNA-binding protein Whi3 is a key regulator of developmental signaling and ploidy in Saccharomyces cerevisiae. Genetics. 2013;195:73-86 pubmed publisher..In summary, our study suggests that the RNA-binding protein Whi3 acts as a central regulator of cell division and development by post-transcriptional control of key genes involved in chromosome distribution and cell signaling. ..
- Hahn M, Jüngling S, Knogge W. Cultivar-specific elicitation of barley defense reactions by the phytotoxic peptide NIP1 from Rhynchosporium secalis. Mol Plant Microbe Interact. 1993;6:745-54 pubmed..Therefore, NIP1 is a candidate for the product of fungal avirulence gene avrRrs1, which, together with barley resistance gene Rrs1, determines incompatibility of the interaction. ..
- Moczko M, Ehmann B, Gartner F, Hönlinger A, Schafer E, Pfanner N. Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J Biol Chem. 1994;269:9045-51 pubmed..Interestingly, the primary sequence of MOM19 predicts the presence of a tetratricopeptide motif that was also found in MOM72, in the peroxisomal membrane protein PAS8/PAS10, and in several proteins involved in RNA synthesis or mitosis. ..
- Knop M, Hauser N, Wolf D. N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast. Yeast. 1996;12:1229-38 pubmed..Our results suggest that the ER of yeast contains a glycosylation-dependent quality control system, as has been shown for higher eukaryotic cells. ..
- Münchow S, Sauter C, Jansen R. Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins. J Cell Sci. 1999;112 ( Pt 10):1511-8 pubmed..This strongly suggests a direct role of Myo4p myosin as a transporter of localised mRNAs, convincingly supporting the concept of motor-protein based mRNA localisation. ..
- Koch C, Wollmann P, Dahl M, Lottspeich F. A role for Ctr9p and Paf1p in the regulation G1 cyclin expression in yeast. Nucleic Acids Res. 1999;27:2126-34 pubmed..Paf1p and Cdc73p were previously reported to be RNA poly-merase II-associated proteins, suggesting that the Ctr9p complex may interact with the general transcription apparatus. ..
- Strasser K, Hurt E. Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. EMBO J. 2000;19:410-20 pubmed..Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes. ..
- Peng R, De Antoni A, Gallwitz D. Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members. J Biol Chem. 2000;275:11521-8 pubmed..The data presented strongly suggest that the Sec24p-related proteins function as COPII components. ..
- Poirey R, Despons L, Leh V, Lafuente M, Potier S, Souciet J, et al. Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability. Microbiology. 2002;148:2111-23 pubmed..Two-hybrid screens, hSos1p fusions and GFP fusions were carried out; the results indicated that the Dup240 proteins are membrane associated, and that some of them are concentrated around the plasma membrane. ..
- Stein K, Schell Steven A, Erdmann R, Rottensteiner H. Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol. 2002;22:6056-69 pubmed..This complex accumulated in docking mutants but was absent in cells lacking Pex18p/Pex21p, indicating that Pex18p/Pex21p are required already before the docking event. ..
- Luo Z, Gallwitz D. Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem. 2003;278:791-9 pubmed..The results obtained indicate that Ypt6p acts in endosome-to-Golgi, in intra-Golgi retrograde transport, and possibly also in Golgi-to-ER trafficking. ..
- Mokranjac D, Paschen S, Kozany C, Prokisch H, Hoppins S, Nargang F, et al. Tim50, a novel component of the TIM23 preprotein translocase of mitochondria. EMBO J. 2003;22:816-25 pubmed..We suggest that Tim50 plays a crucial role in the transfer of preproteins from the TOM complex to the TIM23 complex through the intermembrane space. ..
- Mesecke N, Terziyska N, Kozany C, Baumann F, Neupert W, Hell K, et al. A disulfide relay system in the intermembrane space of mitochondria that mediates protein import. Cell. 2005;121:1059-69 pubmed..We suggest that this process reflects the evolutionary origin of the IMS from the periplasmic space of the prokaryotic ancestors of mitochondria. ..
- Krappmann S, Jung N, Medic B, Busch S, Prade R, Braus G. The Aspergillus nidulans F-box protein GrrA links SCF activity to meiosis. Mol Microbiol. 2006;61:76-88 pubmed
- Hofacker M, Gompf S, Zutz A, Presenti C, Haase W, van der Does C, et al. Structural and functional fingerprint of the mitochondrial ATP-binding cassette transporter Mdl1 from Saccharomyces cerevisiae. J Biol Chem. 2007;282:3951-61 pubmed..By single particle electron microscopy, a first three-dimensional structure of the mitochondrial ATP-binding cassette transporter was derived at 2.3-nm resolution, revealing a homodimeric complex in an open conformation...
- Gnad F, de Godoy L, Cox J, Neuhauser N, Ren S, Olsen J, et al. High-accuracy identification and bioinformatic analysis of in vivo protein phosphorylation sites in yeast. Proteomics. 2009;9:4642-52 pubmed publisher..We constructed a yeast-specific phosphorylation sites predictor on the basis of a support vector machine, which - together with the yeast phosphorylation data - is integrated into the PHOSIDA database (www.phosida.com). ..
- Ernst R, Kueppers P, Stindt J, Kuchler K, Schmitt L. Multidrug efflux pumps: substrate selection in ATP-binding cassette multidrug efflux pumps--first come, first served?. FEBS J. 2010;277:540-9 pubmed publisher..We propose that substrate selection in eukaryotic multidrug ATP-binding cassette transporters is not solely determined by structural features of the transmembrane domains but also by their dynamic behavior. ..
- Heise B, van der Felden J, Kern S, Malcher M, Bruckner S, Mösch H. The TEA transcription factor Tec1 confers promoter-specific gene regulation by Ste12-dependent and -independent mechanisms. Eukaryot Cell. 2010;9:514-31 pubmed publisher..Our study suggests that Tec1 is able to regulate TCS-mediated gene expression by Ste12-dependent and Ste12-independent mechanisms that enable promoter-specific transcriptional control. ..
- Muhlenhoff U, Molik S, Godoy J, Uzarska M, Richter N, Seubert A, et al. Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster. Cell Metab. 2010;12:373-385 pubmed publisher..Collectively, our study uncovers an important role of monothiol glutaredoxins in cellular iron metabolism, with a surprising connection to cellular redox and sulfur metabolisms. ..
- Schmid A, Lagleder S, Gräwert M, Röhl A, Hagn F, Wandinger S, et al. The architecture of functional modules in the Hsp90 co-chaperone Sti1/Hop. EMBO J. 2012;31:1506-17 pubmed publisher..Both domains also interact with the Hsp90 middle domain. The accessory TPR1-DP1 module may serve as an Hsp70-client delivery system for the TPR2A-TPR2B-DP2 segment, which is required for client activation in vivo. ..
- Perzlmaier A, Richter F, Seufert W. Translation initiation requires cell division cycle 123 (Cdc123) to facilitate biogenesis of the eukaryotic initiation factor 2 (eIF2). J Biol Chem. 2013;288:21537-46 pubmed publisher..This work identifies a novel step in the eukaryotic translation initiation pathway and assigns a biochemical function to a protein that is essential for growth and viability of eukaryotic cells. ..
- Paret C, Ostermann K, Krause Buchholz U, Rentzsch A, Rödel G. Human members of the SCO1 gene family: complementation analysis in yeast and intracellular localization. FEBS Lett. 1999;447:65-70 pubmed..Interestingly, the respective chimera with the human homolog of chromosome 22 is not able to complement. Expression of EGFP fusions in HeLa cells shows that both human ScSco1p homologs are located in the mitochondria of human cells. ..
- Krimmer T, Rassow J, Kunau W, Voos W, Pfanner N. Mitochondrial protein import motor: the ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the peptide binding domain and the carboxy-terminal segment play a stimulatory role. Mol Cell Biol. 2000;20:5879-87 pubmed..We conclude that the ATPase domain of mtHsp70 is essential for and directly interacts with Tim44, clearly separating the mtHsp70-Tim44 interaction from the mtHsp70-substrate interaction. ..
- Pelzer W, Muhlenhoff U, Diekert K, Siegmund K, Kispal G, Lill R. Mitochondrial Isa2p plays a crucial role in the maturation of cellular iron-sulfur proteins. FEBS Lett. 2000;476:134-9 pubmed..Thus, Isa2p is a new member of the Fe/S cluster biosynthesis machinery of the mitochondrial matrix and may be involved in the binding of an intermediate of Fe/S cluster assembly. ..
- Gruneberg U, Glotzer M, Gartner A, Nigg E. The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo. J Cell Biol. 2002;158:901-14 pubmed..These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism. ..
- Breinig F, Schleinkofer K, Schmitt M. Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture. Microbiology. 2004;150:3209-18 pubmed..Taken together, these data favour and suggest a structural, rather than enzymic, function of Kre1p in yeast cell wall assembly. ..
- Galani K, Hurt E, Simos G. The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanism. FEBS Lett. 2005;579:969-75 pubmed..Thus, the cytoplasmic location of Arc1p is maintained by Xpo1p-dependent nuclear export and Arc1p could act as an adapter in the nucleocytoplasmic trafficking of tRNA and/or the tRNA-aminoacylation machinery. ..
- Krause Buchholz U, Schöbel K, Lauffer S, Rödel G. Saccharomyces cerevisiae translational activator Cbs1p is associated with translationally active mitochondrial ribosomes. Biol Chem. 2005;386:407-15 pubmed..Our data indicate that the observed interaction is mediated by ribosome-bound mRNA, thus restricting the association to ribosomes actively translating cytochrome b. ..
- Makino D, Conti E. Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement. Acta Crystallogr D Biol Crystallogr. 2013;69:2226-35 pubmed publisher..Here, the crystallographic steps towards the structural elucidation, which was carried out by molecular replacement, are presented. ..
- Matsuo Y, Granneman S, Thoms M, Manikas R, Tollervey D, Hurt E. Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export. Nature. 2014;505:112-116 pubmed publisher..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
- Merlin A, Voos W, Maarse A, Meijer M, Pfanner N, Rassow J. The J-related segment of tim44 is essential for cell viability: a mutant Tim44 remains in the mitochondrial import site, but inefficiently recruits mtHsp70 and impairs protein translocation. J Cell Biol. 1999;145:961-72 pubmed..The efficient cooperation of mtHsp70 with Tim44 facilitates the translocation of loosely folded preproteins and plays a crucial role in the import of preproteins which contain a tightly folded domain. ..
- Schwab M, Neutzner M, Mocker D, Seufert W. Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC. EMBO J. 2001;20:5165-75 pubmed..We propose that proteins of the Cdc20 family are substrate recognition subunits of the ubiquitin ligase APC. ..
- Huang X, Hetfeld B, Seifert U, Kahne T, Kloetzel P, Naumann M, et al. Consequences of COP9 signalosome and 26S proteasome interaction. FEBS J. 2005;272:3909-17 pubmed..The possible role of super-complexes composed of the CSN, the 26S proteasome and of Ub ligases in the regulation of protein stability is discussed. ..
- Chen S, Davies A, Sagan D, Ulrich H. The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent manner. Nucleic Acids Res. 2005;33:5878-86 pubmed
- Kuhnke G, Neumann K, Muhlenhoff U, Lill R. Stimulation of the ATPase activity of the yeast mitochondrial ABC transporter Atm1p by thiol compounds. Mol Membr Biol. 2006;23:173-84 pubmed..We speculate that the physiological substrate of Atm1p may contain multiple sulfhydryl groups in a peptidic environment...
- Osman C, Wilmes C, Tatsuta T, Langer T. Prohibitins interact genetically with Atp23, a novel processing peptidase and chaperone for the F1Fo-ATP synthase. Mol Biol Cell. 2007;18:627-35 pubmed..Our results therefore characterize not only a novel processing peptidase with chaperone activity in the mitochondrial intermembrane space but also link the function of prohibitins to the F(1)F(O)-ATP synthase complex. ..
- Ragni E, Sipiczki M, Strahl S. Characterization of Ccw12p, a major key player in cell wall stability of Saccharomyces cerevisiae. Yeast. 2007;24:309-19 pubmed..However, loss of N-glycans does not affect Ccw12p functionality. In contrast, deletion of the repeated amino acid motive TTEAPKNGTSTAAP in the C-terminal part of the protein affects Ccw12p function. ..
- Becker T, Pfannschmidt S, Guiard B, Stojanovski D, Milenkovic D, Kutik S, et al. Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors. J Biol Chem. 2008;283:120-7 pubmed..Thus, Mim1 plays an important role in the efficient assembly of the mitochondrial TOM complex. ..
- Lorentzen E, Basquin J, Tomecki R, Dziembowski A, Conti E. Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family. Mol Cell. 2008;29:717-28 pubmed publisher..The structure provides a molecular rationale for the observed biochemical properties of the RNase R family of nucleases. ..
- Malcher M, Schladebeck S, Mösch H. The Yak1 protein kinase lies at the center of a regulatory cascade affecting adhesive growth and stress resistance in Saccharomyces cerevisiae. Genetics. 2011;187:717-30 pubmed publisher..In summary, our data suggest that Yak1 is at the center of a regulatory cascade for adhesive growth and stress resistance, which is under dual control of Whi3 and the PKA subunit Tpk1. ..
- Halbach F, Rode M, Conti E. The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome. RNA. 2012;18:124-34 pubmed publisher..We speculate that the Ski2 and Mtr4 insertion domains have evolved with different properties tailored to the type of transcripts that are the substrates of the cytoplasmic and nuclear exosome. ..
- Tarasenko D, Barbot M, Jans D, Kroppen B, Sadowski B, Heim G, et al. The MICOS component Mic60 displays a conserved membrane-bending activity that is necessary for normal cristae morphology. J Cell Biol. 2017;216:889-899 pubmed publisher..Our results show that membrane bending by Mic60 is an ancient mechanism, important for cristae formation, and had already evolved before Î±-proteobacteria developed into mitochondria. ..
- Lill R, Kispal G. Mitochondrial ABC transporters. Res Microbiol. 2001;152:331-40 pubmed..The function of the mitochondrial Mdl1/2p-like proteins is not clear at present with the notable exception of murine ABC-me that may transport intermediates of heme biosynthesis from the matrix to the cytosol in erythroid tissues...
- Mey G, Oeser B, Lebrun M, Tudzynski P. The biotrophic, non-appressorium-forming grass pathogen Claviceps purpurea needs a Fus3/Pmk1 homologous mitogen-activated protein kinase for colonization of rye ovarian tissue. Mol Plant Microbe Interact. 2002;15:303-12 pubmed..Although both fungi drastically differ in their pathogenic strategies, this result indicates that the signal pathway involving CPMK1 is highly conserved...
- Hainzl O, Wegele H, Richter K, Buchner J. Cns1 is an activator of the Ssa1 ATPase activity. J Biol Chem. 2004;279:23267-73 pubmed..Taken together, Cns1 is a potent cochaperone of Ssa1. Our findings highlight the importance of the regulation of Hsp70 function in the context of the Hsp90 chaperone cycle. ..
- Schliebs W, Girzalsky W, Erdmann R. Peroxisomal protein import and ERAD: variations on a common theme. Nat Rev Mol Cell Biol. 2010;11:885-90 pubmed publisher..We propose that the ER-associated protein degradation (ERAD)-like removal of the peroxisomal import receptor is mechanically coupled to protein translocation into the organelle, giving rise to a new concept of export-driven import...
- ZiÃ³Å‚kowska N, Karotki L, Rehman M, Huiskonen J, Walther T. Eisosome-driven plasma membrane organization is mediated by BAR domains. Nat Struct Mol Biol. 2011;18:854-6 pubmed publisher..Our data reveal a previously unrecognized family of BAR-domain proteins involved in plasma membrane organization. ..
- Heininger A, Hackert P, Andreou A, Boon K, Memet I, Prior M, et al. Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol. 2016;13:320-30 pubmed publisher..Together, these findings suggest that the availability of cofactors and the sequestering of the helicase are means to regulate the activity of multifunctional RNA helicases and their distribution between different cellular processes. ..
- van Rossum H, Kozak B, Niemeijer M, Duine H, Luttik M, Boer V, et al. Alternative reactions at the interface of glycolysis and citric acid cycle in Saccharomyces cerevisiae. FEMS Yeast Res. 2016;16: pubmed publisher..Strains in which all known reactions at the glycolysis-TCA cycle interface were inactivated still grew slowly on glucose, indicating additional flexibility at this key metabolic junction. ..
- Schork S, Thumm M, Wolf D. Catabolite inactivation of fructose-1,6-bisphosphatase of Saccharomyces cerevisiae. Degradation occurs via the ubiquitin pathway. J Biol Chem. 1995;270:26446-50 pubmed..Catabolite degradation of FBPase is dependent on the ubiquitin-conjugating enzymes Ubc1, Ubc4, and Ubc5. The 26 S proteasome is involved in the degradation process. ..
- Jaksch M, Horvath R, Horn N, Auer D, MacMillan C, Peters J, et al. Homozygosity (E140K) in SCO2 causes delayed infantile onset of cardiomyopathy and neuropathy. Neurology. 2001;57:1440-6 pubmed..There is increased copper uptake in patients' fibroblasts indicating that the G1541A mutation effects cellular copper metabolism. ..
- Galani K, Grosshans H, Deinert K, Hurt E, Simos G. The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p. EMBO J. 2001;20:6889-98 pubmed..We suggest that the organization of aminoacyl-tRNA synthetases into a multimeric complex not only affects catalysis, but is also a means of segregating the tRNA- aminoacylation machinery mainly to the cytoplasmic compartment. ..
- Pruchner D, Beckert S, Muhle H, Knoop V. Divergent intron conservation in the mitochondrial nad2 gene: signatures for the three bryophyte classes (mosses, liverworts, and hornworts) and the lycophytes. J Mol Evol. 2002;55:265-71 pubmed..Plant mitochondrial peculiarities such as RNA editing frequency, U-to-C type of RNA editing, and small group II introns appear to be genus-specific rather than gene-specific features. ..
- De Antoni A, Schmitzová J, Trepte H, Gallwitz D, Albert S. Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs. J Biol Chem. 2002;277:41023-31 pubmed..The data indicate that hydrolysis of Ypt1p-bound GTP is a prerequisite for a balanced vesicle flow between endoplasmic reticulum and Golgi compartments. ..
- Dilcher M, Veith B, Chidambaram S, Hartmann E, Schmitt H, Fischer von Mollard G. Use1p is a yeast SNARE protein required for retrograde traffic to the ER. EMBO J. 2003;22:3664-74 pubmed..Therefore, we conclude that Use1p is a novel SNARE protein that functions in retrograde traffic from the Golgi to the ER. ..
- Fischer T, Rodriguez Navarro S, Pereira G, Rácz A, Schiebel E, Hurt E. Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat Cell Biol. 2004;6:840-8 pubmed..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery. ..
- Rakwalska M, Rospert S. The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:9186-97 pubmed..Based on the combined data we conclude that RAC and Ssb1/2p are crucial in maintaining translational fidelity beyond their postulated role as chaperones for nascent polypeptides. ..
- Habib S, Waizenegger T, Lech M, Neupert W, Rapaport D. Assembly of the TOB complex of mitochondria. J Biol Chem. 2005;280:6434-40 pubmed..Mas37 assembled directly with the TOB core complex. Hence, the biogenesis of Mas37 represents a novel import pathway of mitochondrial proteins. ..
- Reihl P, Stolz J. The monocarboxylate transporter homolog Mch5p catalyzes riboflavin (vitamin B2) uptake in Saccharomyces cerevisiae. J Biol Chem. 2005;280:39809-17 pubmed..cerevisiae has a mechanism to sense riboflavin and avert riboflavin deficiency by increasing the expression of the plasma membrane transporter MCH5. Moreover, the other members of the MCH gene family appear to have unrelated functions. ..
- Wittig I, Schagger H. Structural organization of mitochondrial ATP synthase. Biochim Biophys Acta. 2008;1777:592-8 pubmed publisher..Independent approaches are presented that support previous reports on the existence of ATP synthasomes in the mitochondrial membrane. ..
- Aso Y, Grübel K, Busch S, Friedrich A, Siwanowicz I, Tanimoto H. The mushroom body of adult Drosophila characterized by GAL4 drivers. J Neurogenet. 2009;23:156-72 pubmed publisher..Taken together, this study lays groundwork for functional dissection of the mushroom body...
- Stehling O, Vashisht A, Mascarenhas J, Jonsson Z, Sharma T, Netz D, et al. MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science. 2012;337:195-9 pubmed publisher..The function of MMS19 in the maturation of crucial components of DNA metabolism may explain the sensitivity of MMS19 mutants to DNA damage and the presence of extended telomeres. ..
- Sirrenberg C, Bauer M, Guiard B, Neupert W, Brunner M. Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22. Nature. 1996;384:582-5 pubmed..Import of proteins of the AAC family is independent of Tim23, and import of matrix targeting signals containing preproteins is independent of Tim22. ..
- Farkasovsky M, Küntzel H. Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast. J Cell Biol. 2001;152:251-62 pubmed..In addition, Num1p may transiently cooperate with an associated Bni1p-Kar9p complex at the bud tip of early anaphase cells. ..
- Scheglmann D, Werner K, Eiselt G, Klinger R. Role of paired basic residues of protein C-termini in phospholipid binding. Protein Eng. 2002;15:521-8 pubmed..This mechanism could be an additional explanation for the C-terminal amino acid bias observed in proteins of several species. ..
- Messerschmitt M, Jakobs S, Vogel F, Fritz S, Dimmer K, Neupert W, et al. The inner membrane protein Mdm33 controls mitochondrial morphology in yeast. J Cell Biol. 2003;160:553-64 pubmed..Our results indicate that Mdm33 plays a distinct role in the mitochondrial inner membrane to control mitochondrial morphology. We propose that Mdm33 is involved in fission of the mitochondrial inner membrane. ..
- Stephan J, Stemmer V, Niederweis M. Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase. Gene. 2004;343:181-90 pubmed..These results show that the Flp/FRT system is a suitable genetic tool for constructing unmarked mutations and for the analysis of redundant genes by consecutive gene deletions in M. smegmatis. ..
- Willer T, Brandl M, Sipiczki M, Strahl S. Protein O-mannosylation is crucial for cell wall integrity, septation and viability in fission yeast. Mol Microbiol. 2005;57:156-70 pubmed..pombe oma1Delta and oma4Delta single mutants showed that a lack of O-mannosylation results in abnormal cell wall and septum formation, thereby severely affecting cell morphology and cell-cell separation. ..
- Rumpf S, Jentsch S. Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone. Mol Cell. 2006;21:261-9 pubmed..We propose that the balance between the distinct substrate-processing cofactors may determine whether a substrate is multiubiquitylated and routed to the proteasome for degradation or deubiquitylated and/or released for other purposes. ..
- Mokranjac D, Berg A, Adam A, Neupert W, Hell K. Association of the Tim14.Tim16 subcomplex with the TIM23 translocase is crucial for function of the mitochondrial protein import motor. J Biol Chem. 2007;282:18037-45 pubmed..Deletion of hydrophobic segments in both, Tim16 and Tim14, is lethal. We conclude that import into the matrix space of mitochondria requires association of the co-chaperones Tim16 and Tim14 with the TIM23 preprotein translocase. ..
- Wittig I, Velours J, Stuart R, Schagger H. Characterization of domain interfaces in monomeric and dimeric ATP synthase. Mol Cell Proteomics. 2008;7:995-1004 pubmed publisher..In contrast to the monomer-monomer interface, the interface between dimers in higher oligomeric structures remains largely unknown. However, we could show that the natural inhibitor protein Inh1 is not required for oligomerization. ..
- Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher..In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability. ..
- Loeber J, Pan F, Pieler T. Generation of transgenic frogs. Methods Mol Biol. 2009;561:65-72 pubmed publisher..Tissue-specific gene expression, as well as germline transmission, has been observed. ..
- Hahn S, Schlenstedt G. Importin ?-type nuclear transport receptors have distinct binding affinities for Ran-GTP. Biochem Biophys Res Commun. 2011;406:383-8 pubmed publisher..We found that the number of ?-receptors altogether about equals the amounts of yeast Ran, but Ran-GTP is not limiting in the nucleus. The implications of our results for nucleocytoplasmic transport mechanisms are discussed. ..
- Hoffmann C, Neumann H. In Vivo Mapping of FACT-Histone Interactions Identifies a Role of Pob3 C-terminus in H2A-H2B Binding. ACS Chem Biol. 2015;10:2753-63 pubmed publisher..Deletion of acidic residues from the Pob3 C-terminus creates a hydroxyurea-sensitive phenotype in budding yeast, suggesting a potential role for this domain in DNA replication. ..
- Wittke S, Dunnwald M, Johnsson N. Sec62p, a component of the endoplasmic reticulum protein translocation machinery, contains multiple binding sites for the Sec-complex. Mol Biol Cell. 2000;11:3859-71 pubmed..The C-terminal binding site of Sec62p is less important for complex stability, but adjoins the region in Sec62p that might be involved in signal sequence recognition. ..
- Rothbauer U, Hofmann S, Mühlenbein N, Paschen S, Gerbitz K, Neupert W, et al. Role of the deafness dystonia peptide 1 (DDP1) in import of human Tim23 into the inner membrane of mitochondria. J Biol Chem. 2001;276:37327-34 pubmed..Thus, the pathomechanism underlying the Mohr-Tranebjaerg syndrome may involve an impaired biogenesis of the human TIM23 complex causing severe pleiotropic mitochondrial dysfunction. ..
- Scharnewski M, Pongdontri P, Mora G, Hoppert M, Fulda M. Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling. FEBS J. 2008;275:2765-78 pubmed publisher..Therefore, we propose the recycling of endogenous fatty acids generated in the course of lipid remodelling as a major task of both acyl-CoA synthetases Faa1p and Faa4p. ..
- Wolter R, Richter D, Niegemann E, Brendel M. Molecular characterisation of GTP1, a Saccharomyces cerevisiae gene encoding a small GTP-binding protein. Curr Genet. 1994;26:564-6 pubmed..Homology within GTP-binding motifs is highly conserved. Gene disruption showed that GTP1 is not an essential gene and that it has no influence on the expression of the DNA repair gene SNM1 with which it shares a 191-bp promoter region. ..