Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in France
Topic: saccharomyces cerevisiae proteins
Publications366 found, 100 shown here
- Fourel G, Miyake T, Defossez P, Li R, Gilson E. General regulatory factors (GRFs) as genome partitioners. J Biol Chem. 2002;277:41736-43 pubmed..Thus GRFs, which bind to sites scattered throughout the genome within promoters, would not only play a key role in regulating gene expression but also partition the genome in functionally independent domains. ..
- Lafleuriel J, Degroote F, Depeiges A, Picard G. Impact of the loss of AtMSH2 on double-strand break-induced recombination between highly diverged homeologous sequences in Arabidopsis thaliana germinal tissues. Plant Mol Biol. 2007;63:833-46 pubmed..In addition, conversion tracts were observed in some alleles. They are shorter in MSH2 deficient plants than in wild-type, suggesting that a short-patch mismatch repair, not controlled by MSH2, could exist in Arabidopsis. ..
- Ruault M, De Meyer A, Loiodice I, Taddei A. Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast. J Cell Biol. 2011;192:417-31 pubmed publisher..We propose that similar mechanisms involving proteins able to oligomerize account for long-range interactions that impact genomic functions in many organisms. ..
- Thomas D, Becker A, Surdin Kerjan Y. Reverse methionine biosynthesis from S-adenosylmethionine in eukaryotic cells. J Biol Chem. 2000;275:40718-24 pubmed..New pathways for AdoMet or S-methylmethionine-dependent methionine synthesis are presented. ..
- Remize F, Barnavon L, Dequin S. Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. Metab Eng. 2001;3:301-12 pubmed..Overexpression of GPD1 in this strain increased the amount of glycerol produced but led to a pronounced growth defect. ..
- Gadal O, Labarre S, Boschiero C, Thuriaux P. Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system. EMBO J. 2002;21:5498-507 pubmed..Conversely, rpa34-Delta mutants lacking Rpa34, a non-essential subunit associated with Rpa49, are lethal in top1-Delta but not in top3-Delta. ..
- Vial L, Gomez P, Panvert M, Schmitt E, Blanquet S, Mechulam Y. Mitochondrial methionyl-tRNAfMet formyltransferase from Saccharomyces cerevisiae: gene disruption and tRNA substrate specificity. Biochemistry. 2003;42:932-9 pubmed..Each mechanism appears well suited to an efficient selection of the substrate within the pool of all tRNAs. ..
- Antonny B, Gounon P, Schekman R, Orci L. Self-assembly of minimal COPII cages. EMBO Rep. 2003;4:419-24 pubmed..We suggest that these structures, which represent a minimal COPII cage, mimic the molecular organization of the membrane-associated COPII coat...
- Eluère R, Offner N, Varlet I, Motteux O, Signon L, Picard A, et al. Compartmentalization of the functions and regulation of the mitotic cyclin Clb2 in S. cerevisiae. J Cell Sci. 2007;120:702-11 pubmed..Altogether, our results demonstrate that the subcellular localization of the mitotic cyclin Clb2 is one of the key determinants of its biological function. ..
- Gombault A, Godin F, Sy D, Legrand B, Chautard H, Vallee B, et al. Molecular basis of the Tfs1/Ira2 interaction: a combined protein engineering and molecular modelling study. J Mol Biol. 2007;374:604-17 pubmed
- Kerrest A, Anand R, Sundararajan R, Bermejo R, Liberi G, Dujon B, et al. SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombination. Nat Struct Mol Biol. 2009;16:159-67 pubmed publisher..In the absence of Srs2 or Sgs1, DNA damage accumulates and is processed by homologous recombination, triggering repeat rearrangements...
- Lefevre S, Sliwa D, Auchère F, Brossas C, Ruckenstuhl C, Boggetto N, et al. The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells. FEBS Lett. 2012;586:143-8 pubmed publisher..In addition, our results suggest that metacaspase is associated with mitochondrial respiration, intracellular redox control and genomic stability. ..
- Faure G, Callebaut I. Identification of hidden relationships from the coupling of hydrophobic cluster analysis and domain architecture information. Bioinformatics. 2013;29:1726-33 pubmed publisher..7 and is available on request. Instructions are available at http://www.impmc.upmc.fr/?callebau/tremolohca.html. email@example.com Supplementary Data are available at Bioinformatics online. ..
- Mylona A, Acker J, Fernández Tornero C, Sentenac A, Müller C. Expression, proteolytic analysis, reconstitution, and crystallization of the tau60/tau91 subcomplex of yeast TFIIIC. Protein Expr Purif. 2006;45:255-61 pubmed..Crystals diffracting beyond 3.2 A were obtained from a complex formed by full-length tau60 and the N-terminally truncated form of tau91 lacking this extension. ..
- Picaud T, Desbois A. Interaction of glutathione reductase with heavy metal: the binding of Hg(II) or Cd(II) to the reduced enzyme affects both the redox dithiol pair and the flavin. Biochemistry. 2006;45:15829-37 pubmed..The bound metal ion then acts on the bending of the isoalloxazine ring of FAD as well as on the hydrophobicity of its microenvironment. ..
- Peyroche G, Milkereit P, Bischler N, Tschochner H, Schultz P, Sentenac A, et al. The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J. 2000;19:5473-82 pubmed..The existence of mammalian orthologues of A43 and Rrn3 suggests evolutionary conservation of the molecular mechanisms underlying rDNA transcription in eukaryotes. ..
- Martin Yken H, Dagkessamanskaia A, de Groot P, Ram A, Klis F, Francois J. Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway. Yeast. 2001;18:827-40 pubmed
- Hutin C, Havaux M, Carde J, Kloppstech K, Meiherhoff K, Hoffman N, et al. Double mutation cpSRP43--/cpSRP54-- is necessary to abolish the cpSRP pathway required for thylakoid targeting of the light-harvesting chlorophyll proteins. Plant J. 2002;29:531-43 pubmed..Phenotypic analysis of the double mutant, compared to the single mutants, suggests that the cpSRP subunits cpSRP43 and cpSRP54 contribute to antenna targeting in an independent but additive way. ..
- Biteau B, Labarre J, Toledano M. ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin. Nature. 2003;425:980-4 pubmed..Sulphiredoxin is important for the antioxidant function of peroxiredoxins, and is likely to be involved in the repair of proteins containing cysteine-sulphinic acid modifications, and in signalling pathways involving protein oxidation. ..
- Vanrobays E, Gelugne J, Caizergues Ferrer M, Lafontaine D. Dim2p, a KH-domain protein required for small ribosomal subunit synthesis. RNA. 2004;10:645-56 pubmed
- Schwimmer C, Lefebvre Legendre L, Rak M, Devin A, Slonimski P, di Rago J, et al. Increasing mitochondrial substrate-level phosphorylation can rescue respiratory growth of an ATP synthase-deficient yeast. J Biol Chem. 2005;280:30751-9 pubmed..This unexpected result might be of interest for the development of therapeutic solutions in pathologies associated with defects in the oxidative phosphorylation system. ..
- Chevtzoff C, Yoboue E, Galinier A, Casteilla L, Daignan Fornier B, Rigoulet M, et al. Reactive oxygen species-mediated regulation of mitochondrial biogenesis in the yeast Saccharomyces cerevisiae. J Biol Chem. 2010;285:1733-42 pubmed publisher..It is the first time that a redox sensitivity of the transcription factors involved in yeast mitochondrial biogenesis is shown. Such a process could be seen as a mitochondria quality control process. ..
- Rigoulet M, Mourier A, Galinier A, Casteilla L, Devin A. Electron competition process in respiratory chain: regulatory mechanisms and physiological functions. Biochim Biophys Acta. 2010;1797:671-7 pubmed publisher..Indeed, the more reduced pool would be associated to the electron right of way for the external dehydrogenases whereas the less reduced pool would be associated to the electron right of way for the internal dehydrogenases. ..
- Beckouët F, Mariotte Labarre S, Peyroche G, Nogi Y, Thuriaux P. Rpa43 and its partners in the yeast RNA polymerase I transcription complex. FEBS Lett. 2011;585:3355-9 pubmed publisher..Two-hybrid data and other genetic evidence suggest that Rpa43 directly bind Spt5, an RNAPI elongation factor also acting in RNAPII-dependent transcription, and may also interact with the nucleosomal chaperone Spt6. ..
- Steyer D, Erny C, Claudel P, Riveill G, Karst F, Legras J. Genetic analysis of geraniol metabolism during fermentation. Food Microbiol. 2013;33:228-34 pubmed publisher..Moreover lab strain over-expressing OYE2 allows 87% geraniol to citronellol reduction in bioconversion experiment compared to about 50% conversion with control strain. ..
- Defenouillère Q, Yao Y, Mouaikel J, Namane A, Galopier A, Decourty L, et al. Cdc48-associated complex bound to 60S particles is required for the clearance of aberrant translation products. Proc Natl Acad Sci U S A. 2013;110:5046-51 pubmed publisher..We propose that ubiquitination of the nascent peptide starts on the 80S and continues on the 60S, on which Cdc48 is recruited to escort the substrate for proteasomal degradation. ..
- Muñoz Centeno M, McBratney S, Monterrosa A, Byers B, Mann C, Winey M. Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Mol Biol Cell. 1999;10:2393-406 pubmed..Additionally, immunoelectron microscopy shows that GFP-Mps2p localizes to the SPB. Our analysis suggests that Mps2p is required as a component of the SPB for insertion of the nascent SPB into the nuclear envelope. ..
- Crechet J, Jacquet E, Bernardi A, Parmeggiani A. Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase. J Biol Chem. 2000;275:17754-61 pubmed..The use of Ha-Ras/Ras2p chimaeras of different length emphasized the key role of the hypervariable region of Ras2p in inducing maximum activation of adenylyl cyclase and for a productive interaction with membrane-bound GEF. ..
- Dilworth F, Fromental Ramain C, Yamamoto K, Chambon P. ATP-driven chromatin remodeling activity and histone acetyltransferases act sequentially during transactivation by RAR/RXR In vitro. Mol Cell. 2000;6:1049-58 pubmed..Thus, both ATP-driven chromatin remodeling and HAT activities act in a temporally ordered and interdependent manner to alleviate the repressive effects of nucleosomal histones on transcription by RARalpha/RXRalpha heterodimers. ..
- Eugster A, Lanzuolo C, Bonneton M, Luciano P, Pollice A, Pulitzer J, et al. The finger subdomain of yeast telomerase cooperates with Pif1p to limit telomere elongation. Nat Struct Mol Biol. 2006;13:734-9 pubmed..We propose that, in addition to its catalytic role, the finger subdomain of Est2p facilitates the action of Pif1p at telomeres. ..
- Subileau M, Schneider R, Salmon J, Degryse E. Nitrogen catabolite repression modulates the production of aromatic thiols characteristic of Sauvignon Blanc at the level of precursor transport. FEMS Yeast Res. 2008;8:771-80 pubmed publisher..On grape must, the uptake of the precursor through GAP1p is not confirmed, but the effect of addition of DAP, eventually prolonging NCR, is shown to decrease thiol production. ..
- Ehsani M, Fernández M, Biosca J, Dequin S. Reversal of coenzyme specificity of 2,3-butanediol dehydrogenase from Saccharomyces cerevisae and in vivo functional analysis. Biotechnol Bioeng. 2009;104:381-9 pubmed publisher..In addition, they represent promising tools for the manipulation of the NADP(H) metabolism and for the development of a powerful catalyst in biotransformations requiring NADPH regeneration...
- Laizé V, Gobin R, Rousselet G, Badier C, Hohmann S, Ripoche P, et al. Molecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domain. Biochem Biophys Res Commun. 1999;257:139-44 pubmed..Pf measurements in yeast spheroplasts confirmed the presence of functional water channels in Sigma1278b and a pharmacological study indicated that this strain contains at least a second functional aquaporin. ..
- Peneff C, Mengin Lecreulx D, Bourne Y. The crystal structures of Apo and complexed Saccharomyces cerevisiae GNA1 shed light on the catalytic mechanism of an amino-sugar N-acetyltransferase. J Biol Chem. 2001;276:16328-34 pubmed
- Col E, Caron C, Seigneurin Berny D, Gracia J, Favier A, Khochbin S. The histone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, Tat. J Biol Chem. 2001;276:28179-84 pubmed
- Killian A, Le Meur N, Sesboué R, Bourguignon J, Bougeard G, Gautherot J, et al. Inactivation of the RRB1-Pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Oncogene. 2004;23:8597-602 pubmed
- Leulliot N, Vicentini G, Jordens J, Quevillon Cheruel S, Schiltz M, Barford D, et al. Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity. Mol Cell. 2006;23:413-24 pubmed..Structure-guided mutational studies showed that in vivo PTPA activity is influenced by mutations on the surface of the peptide binding pocket, the same mutations that also influenced the in vitro activation of PP2Ai and PPIase activity. ..
- Romier C, James N, Birck C, Cavarelli J, Vivares C, Collart M, et al. Crystal structure, biochemical and genetic characterization of yeast and E. cuniculi TAF(II)5 N-terminal domain: implications for TFIID assembly. J Mol Biol. 2007;368:1292-306 pubmed
- Zhou L, Le Roux G, Ducrot C, Chédin S, Labarre J, Riva M, et al. Repression of class I transcription by cadmium is mediated by the protein phosphatase 2A. Nucleic Acids Res. 2013;41:6087-97 pubmed publisher..Taken together, our results point to a central role for the Pol I-Rrn3 complex as molecular switch for regulating Pol I transcription in response to toxic metals. ..
- ..Here, we use the modular organization of the evolutionary conserved Spt-Ada-Gcn5 acetyltransferase (SAGA) complex as a paradigm to illustrate how co-activators share and combine a relatively limited set of functional tools. ..
- Volland C, Urban Grimal D, Geraud G, Haguenauer Tsapis R. Endocytosis and degradation of the yeast uracil permease under adverse conditions. J Biol Chem. 1994;269:9833-41 pubmed..We have yet to determine whether adverse conditions enhance permease endocytosis and subsequent degradation or divert internalized uracil permease from a recycling to a degradative pathway. ..
- Truong H, Caboche M, Daniel Vedele F. Sequence and characterization of two Arabidopsis thaliana cDNAs isolated by functional complementation of a yeast gln3 gdh1 mutant. FEBS Lett. 1997;410:213-8 pubmed..RGA1, RGA2 and SCR share several structural features and may define a new family of genes. RGA1 and RGA2 have been mapped, respectively, to chromosome II and I, and their expression in plant is constitutive. ..
- Gangloff Y, Pointud J, Thuault S, Carre L, Romier C, Muratoglu S, et al. The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger. Mol Cell Biol. 2001;21:5109-21 pubmed..These conserved domains are critical for yTAF(II)65 function in vivo. Our results therefore identify metazoan homologues of yTAF(II)47 and yTAF(II)65. ..
- Lambou K, Perkhofer S, Fontaine T, Latge J. Comparative functional analysis of the OCH1 mannosyltransferase families in Aspergillus fumigatus and Saccharomyces cerevisiae. Yeast. 2010;27:625-36 pubmed publisher..Among the four orthologues, only AfOCH1 complemented the aggregation phenotype, the growth inhibition, the hypersensitivity to hygromycin and the protein glycosylation defects observed in the Scoch1Deltamutant. ..
- Carron C, O Donohue M, Choesmel V, Faubladier M, Gleizes P. Analysis of two human pre-ribosomal factors, bystin and hTsr1, highlights differences in evolution of ribosome biogenesis between yeast and mammals. Nucleic Acids Res. 2011;39:280-91 pubmed publisher..These data indicate that ITS1 processing in human cells may be more complex than currently envisioned and that coordination between maturation and nuclear export of pre-40S particles has evolved differently in yeast and mammalian cells. ..
- Séron K, Tieaho V, Prescianotto Baschong C, Aust T, Blondel M, Guillaud P, et al. A yeast t-SNARE involved in endocytosis. Mol Biol Cell. 1998;9:2873-89 pubmed..These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis. ..
- Ischenko A, Saparbaev M. Alternative nucleotide incision repair pathway for oxidative DNA damage. Nature. 2002;415:183-7 pubmed..The 3'-hydroxyl terminus provides the proper end for DNA repair synthesis; the dangling damaged nucleotide on the 5' side is then a good substrate for human flap-structure endonuclease and for DNA polymerase I of E. coli. ..
- Lefebvre Legendre L, Salin B, Schaeffer J, Brèthes D, Dautant A, Ackerman S, et al. Failure to assemble the alpha 3 beta 3 subcomplex of the ATP synthase leads to accumulation of the alpha and beta subunits within inclusion bodies and the loss of mitochondrial cristae in Saccharomyces cerevisiae. J Biol Chem. 2005;280:18386-92 pubmed..These observations are in accord with other studies in the literature that have pointed to a central role for the ATP synthase in biogenesis of the mitochondrial inner membrane. ..
- Deffieu M, Bhatia Kiššová I, Salin B, Galinier A, Manon S, Camougrand N. Glutathione participates in the regulation of mitophagy in yeast. J Biol Chem. 2009;284:14828-37 pubmed publisher..These data show that mitophagy can be regulated independently of general autophagy, and that its implementation may depend on the cellular redox status. ..
- Leclerc V, Tassan J, O Farrell P, Nigg E, Leopold P. Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II. Mol Biol Cell. 1996;7:505-13 pubmed..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function. ..
- Birck C, Poch O, Romier C, Ruff M, Mengus G, Lavigne A, et al. Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family. Cell. 1998;94:239-49 pubmed..The existence of additional histone-like pairs in both the TFIID and SAGA complexes shows that the histone fold is a more commonly used motif for mediating TAF-TAF interactions than previously believed. ..
- Daugeron M, Mauxion F, Seraphin B. The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation. Nucleic Acids Res. 2001;29:2448-55 pubmed..Altogether, our study indicates that Pop2p is a nuclease subunit of the yeast deadenylase and suggests that Pop2p homologues in other species may have similar functions. ..
- Verheggen C, Lafontaine D, Samarsky D, Mouaikel J, Blanchard J, Bordonné R, et al. Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments. EMBO J. 2002;21:2736-45 pubmed..These are likely places for snoRNP assembly, 3' end maturation and cap modification...
- Fernandes H, Roumanie O, Claret S, Gatti X, Thoraval D, Doignon F, et al. The Rho3 and Rho4 small GTPases interact functionally with Wsc1p, a cell surface sensor of the protein kinase C cell-integrity pathway in Saccharomyces cerevisiae. Microbiology. 2006;152:695-708 pubmed..Hence, it is proposed that Wsc1p participates in the regulation of a Rho3/4-dependent cellular mechanism, and that this is distinct from the role of Wsc1p in the PKC-MAP kinase pathway. ..
- Lebreton A, Saveanu C, Decourty L, Rain J, Jacquier A, Fromont Racine M. A functional network involved in the recycling of nucleocytoplasmic pre-60S factors. J Cell Biol. 2006;173:349-60 pubmed..We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation. ..
- Guillemain G, Ma E, Mauger S, Miron S, Thai R, Guerois R, et al. Mechanisms of checkpoint kinase Rad53 inactivation after a double-strand break in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:3378-89 pubmed..Our data thus strongly suggest that CK2 is the kinase responsible for the in vivo phosphorylation of Ptc2 T376. ..
- Gardarin A, Chédin S, Lagniel G, Aude J, Godat E, Catty P, et al. Endoplasmic reticulum is a major target of cadmium toxicity in yeast. Mol Microbiol. 2010;76:1034-48 pubmed publisher..In particular, Cd(2+) activates the calcium channel Cch1/Mid1, which also contributes to Cd(2+) entry into the cell. The results reinforce the interest of using yeast as a cellular model to study toxicity mechanisms in eukaryotic cells. ..
- Ma E, Goldar A, Verbavatz J, Marsolier Kergoat M. Giant yeast cells with nonrecyclable ribonucleotide reductase. Mol Genet Genomics. 2011;285:415-25 pubmed publisher..rnr1C883A rnr3? cells, which can be used as a protein overexpression system, thus illustrate the robustness of S. cerevisiae to multiple physiological parameters. ..
- Meyrial V, Laizé V, Gobin R, Ripoche P, Hohmann S, Tacnet F. Existence of a tightly regulated water channel in Saccharomyces cerevisiae. Eur J Biochem. 2001;268:334-43 pubmed..Our results show that the expression of aquaporins is tightly controlled. The physiological relevance of aquaporin-mediated water transport in yeast is discussed. ..
- Morin Ganet M, Rambourg A, Deitz S, Franzusoff A, Kepes F. Morphogenesis and dynamics of the yeast Golgi apparatus. Traffic. 2000;1:56-68 pubmed..Retention could arise from intra-compartmental flow of cargo/carrier, hinted at by the varying calibers within a single nodular network. ..
- Andrianaivomananjaona T, Moune Dimala M, Herga S, David V, Haraux F. How the N-terminal extremity of Saccharomyces cerevisiae IF1 interacts with ATP synthase: a kinetic approach. Biochim Biophys Acta. 2011;1807:197-204 pubmed publisher..Moreover, the data obtained using chimeric PsaE-IF1 suggest that before binding IF1 presents to the enzyme with its middle part facing a catalytic interface and its Nter extremity folded in the opposite direction. ..
- Yoshida K, Bacal J, Desmarais D, Padioleau I, Tsaponina O, Chabes A, et al. The histone deacetylases sir2 and rpd3 act on ribosomal DNA to control the replication program in budding yeast. Mol Cell. 2014;54:691-7 pubmed publisher..Together, these data indicate that HDACs control the replication timing program in budding yeast by modulating the ability of repeated origins to compete with single-copy origins for limiting initiation factors. ..
- Laporte D, Courtout F, Salin B, Ceschin J, Sagot I. An array of nuclear microtubules reorganizes the budding yeast nucleus during quiescence. J Cell Biol. 2013;203:585-94 pubmed publisher..Finally, mutants that do not assemble a nuclear array of microtubules are impaired for both quiescence survival and exit...
- Maftahi M, Gaillardin C, Nicaud J. Sticky-end polymerase chain reaction method for systematic gene disruption in Saccharomyces cerevisiae. Yeast. 1996;12:859-68 pubmed..g. AscI) specific for the rare restriction site. This approach was used to disrupt three open reading frames identified during the sequencing of COS14-1 from chromosome XIV of S. cerevisiae. ..
- Andrau J, Sentenac A, Werner M. Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34. J Mol Biol. 1999;288:511-20 pubmed..TFIIIC.DNA complexes and affected in vitro transcription of the SUP4 tRNA gene. Our results underscore the important role of region II of TFIIIB70 in pre-initiation as well as transcription complex assembly via C34 and TBP binding. ..
- Bourdineaud J, De Sampaïo G, Lauquin G. A Rox1-independent hypoxic pathway in yeast. Antagonistic action of the repressor Ord1 and activator Yap1 for hypoxic expression of the SRP1/TIR1 gene. Mol Microbiol. 2000;38:879-90 pubmed..Thus, Yap1 is not directly involved in SRP1 induction by hypoxia, but is necessary to counteract the Ord1 effect. ..
- Debrauwere H, Loeillet S, Lin W, Lopes J, Nicolas A. Links between replication and recombination in Saccharomyces cerevisiae: a hypersensitive requirement for homologous recombination in the absence of Rad27 activity. Proc Natl Acad Sci U S A. 2001;98:8263-9 pubmed..This and previous studies indicate that homologous recombination is the primary, but not only, pathway that functions to bypass the replication defects that arise in the absence of the Rad27 protein. ..
- Gendrel C, Dutreix M. (CA/TG) microsatellite sequences escape the inhibition of recombination by mismatch repair in Saccharomyces cerevisiae. Genetics. 2001;159:1539-45 pubmed..We propose that the inhibition of conversion by large inserts is due to recognition by the Msh2/Pms1 complex of mismatches created by intrastrand interactions in the heteroduplex loop. ..
- Brendel C, Gelman L, Auwerx J. Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism. Mol Endocrinol. 2002;16:1367-77 pubmed..MBF-1 seems therefore to act as a bridging factor enabling interactions of nuclear receptors with the transcription machinery. ..
- Cohen M, Stutz F, Dargemont C. Deubiquitination, a new player in Golgi to endoplasmic reticulum retrograde transport. J Biol Chem. 2003;278:51989-92 pubmed..Bre5p complex. All together, our results indicate that the Ubp3p.Bre5p deubiquitination complex co-regulates anterograde and retrograde transports between endoplasmic reticulum and Golgi compartments. ..
- Blondel M, Morvan J, Dupré S, Urban Grimal D, Haguenauer Tsapis R, Volland C. Direct sorting of the yeast uracil permease to the endosomal system is controlled by uracil binding and Rsp5p-dependent ubiquitylation. Mol Biol Cell. 2004;15:883-95 pubmed..Our results are consistent with a model in which the binding of intracellular uracil to the permease signals its sorting from the Golgi apparatus and subsequent ubiquitylation ensures its delivery to the vacuolar lumen. ..
- Tork S, Hatin I, Rousset J, Fabret C. The major 5' determinant in stop codon read-through involves two adjacent adenines. Nucleic Acids Res. 2004;32:415-21 pubmed..We propose that the mRNA structure, depending on the nucleotides in the P site, is the main 5' determinant of read-through efficiency. ..
- Mille C, Janbon G, Delplace F, Ibata Ombetta S, Gaillardin C, Strecker G, et al. Inactivation of CaMIT1 inhibits Candida albicans phospholipomannan beta-mannosylation, reduces virulence, and alters cell wall protein beta-mannosylation. J Biol Chem. 2004;279:47952-60 pubmed..These mechanisms and the impact of the different presentations of beta-oligomannoses on the host response need to be defined. ..
- Morin I, Cuillel M, Lowe J, Crouzy S, Guillain F, Mintz E. Cd2+- or Hg2+-binding proteins can replace the Cu+-chaperone Atx1 in delivering Cu+ to the secretory pathway in yeast. FEBS Lett. 2005;579:1117-23 pubmed..The possible role of electrostatic potential surfaces in the docking of Ccc2 with these Atx1-homologues is discussed. ..
- Dahout Gonzalez C, Ramus C, Dassa E, Dianoux A, Brandolin G. Conformation-dependent swinging of the matrix loop m2 of the mitochondrial Saccharomyces cerevisiae ADP/ATP carrier. Biochemistry. 2005;44:16310-20 pubmed..Its participation in the translocation of ADP/ATP is strongly suggested, based on the finding that its accessibility to reagents added outside mitochondria is modified according to the conformational state of the carrier. ..
- Duval C, Touche V, Tailleux A, Fruchart J, Fievet C, Clavey V, et al. Niemann-Pick C1 like 1 gene expression is down-regulated by LXR activators in the intestine. Biochem Biophys Res Commun. 2006;340:1259-63 pubmed..In conclusion, the present study identifies NPC1L1 as a novel LXR target gene further supporting a crucial role of LXR in intestinal cholesterol homeostasis. ..
- Clémençon B, Rey M, Dianoux A, Trezeguet V, Lauquin G, Brandolin G, et al. Structure-function relationships of the C-terminal end of the Saccharomyces cerevisiae ADP/ATP carrier isoform 2. J Biol Chem. 2008;283:11218-25 pubmed publisher..Taken together, our results highlight the involvement of the Anc2p C-terminal region in nucleotide recognition, binding, and transport. ..
- Mathieu L, Bourens M, Marsy S, Hlavacek O, Panozzo C, Dujardin G. A mutational analysis reveals new functional interactions between domains of the Oxa1 protein in Saccharomyces cerevisiae. Mol Microbiol. 2010;75:474-88 pubmed publisher..This analysis reveals the existence of functional interactions between TM2 and TM5, TM4 and TM5 as well as between TM4 and loop 2, highlighting the key position of TM4 and TM5 in the Oxa1 protein. ..
- Miron S, Durand D, Chilom C, Perez J, Craescu C. Binding of calcium, magnesium, and target peptides to Cdc31, the centrin of yeast Saccharomyces cerevisiae. Biochemistry. 2011;50:6409-22 pubmed publisher..Consequently, Mg(2+) seems to have consequences on Cdc31's function and could be important to stimulate interactions in resting cells. ..
- Marmier Gourrier N, Cléry A, Schlotter F, Senty Ségault V, Branlant C. A second base pair interaction between U3 small nucleolar RNA and the 5'-ETS region is required for early cleavage of the yeast pre-ribosomal RNA. Nucleic Acids Res. 2011;39:9731-45 pubmed publisher..Accordingly, we got strong evidences for a role of segment VI in the association of proteins Mpp10, Imp4 and Imp3. ..
- Ozeir M, Muhlenhoff U, Webert H, Lill R, Fontecave M, Pierrel F. Coenzyme Q biosynthesis: Coq6 is required for the C5-hydroxylation reaction and substrate analogs rescue Coq6 deficiency. Chem Biol. 2011;18:1134-42 pubmed publisher..Our results demonstrate that appropriate analogs of 4-hydroxybenzoic acid can bypass a deficient Q biosynthetic enzyme and might be considered for the treatment of some primary Q deficiencies. ..
- Ma M, Li de la Sierra Gallay I, Lazar N, Pellegrini O, Durand D, Marchfelder A, et al. The crystal structure of Trz1, the long form RNase Z from yeast. Nucleic Acids Res. 2017;45:6209-6216 pubmed publisher..Trz1 likely evolved from a duplication and fusion of the gene encoding the monomeric short form RNase Z. ..
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