Experts and Doctors on saccharomyces cerevisiae proteins in Canada

Summary

Locale: Canada
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Barbosa R, Almeida P, Safar S, Santos R, Morais P, Nielly Thibault L, et al. Evidence of Natural Hybridization in Brazilian Wild Lineages of Saccharomyces cerevisiae. Genome Biol Evol. 2016;8:317-29 pubmed publisher
    ..We hypothesize that hybridization in tropical wild lineages may have facilitated the habitat transition accompanying the colonization of the tropical ecosystem. ..
  2. Lesage G, Shapiro J, Specht C, Sdicu A, Menard P, Hussein S, et al. An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae. BMC Genet. 2005;6:8 pubmed
    ..Our genetic analysis of genes not interacting with CHS3 indicate expanded roles for Chs4p, Chs5p and Chs6p in secretory protein trafficking and of Bni4p in bud neck organization. ..
  3. Bélanger F, Angers J, Fortier Ã, Hammond Martel I, Costantino S, Drobetsky E, et al. Mutations in Replicative Stress Response Pathways Are Associated with S Phase-specific Defects in Nucleotide Excision Repair. J Biol Chem. 2016;291:522-37 pubmed publisher
  4. Bitew T, Sveen C, Heyne B, Zaremberg V. Vitamin E prevents lipid raft modifications induced by an anti-cancer lysophospholipid and abolishes a Yap1-mediated stress response in yeast. J Biol Chem. 2010;285:25731-42 pubmed publisher
    ..Interestingly, the pathway differed from the one triggered by hydrogen peroxide and its activation (measured as Yap1 translocation to the nucleus) was abolished by co-treatment of the cells with alpha-tocopherol. ..
  5. Marco E, Dorn J, Hsu P, Jaqaman K, Sorger P, Danuser G. S. cerevisiae chromosomes biorient via gradual resolution of syntely between S phase and anaphase. Cell. 2013;154:1127-1139 pubmed publisher
    ..Our results offer a revised view of mitotic progression in S. cerevisiae that augments the relevance of mechanistic information obtained in this powerful genetic system for mammalian mitosis. ..
  6. Fairn G, Macdonald K, McMaster C. A chemogenomic screen in Saccharomyces cerevisiae uncovers a primary role for the mitochondria in farnesol toxicity and its regulation by the Pkc1 pathway. J Biol Chem. 2007;282:4868-74 pubmed
    ..The Pkc1 signaling pathway regulates farnesol-mediated cell death through management of the generation of reactive oxygen species. ..
  7. Noël J, Wellinger R. The Smc5/6 complex and the difficulties cutting the ties of twin sisters. Aging (Albany NY). 2011;3:186-8 pubmed
  8. Ratsima H, Serrano D, Pascariu M, D Amours D. Centrosome-Dependent Bypass of the DNA Damage Checkpoint by the Polo Kinase Cdc5. Cell Rep. 2016;14:1422-1434 pubmed publisher
    ..Collectively, our results highlight a previously unappreciated role for centrosomes as key signaling centers for the suppression of cell-cycle arrest induced by persistent or unrepairable DNA damage. ..
  9. Lemire S, Jeromin A, Boisselier Ã. Membrane binding of Neuronal Calcium Sensor-1 (NCS1). Colloids Surf B Biointerfaces. 2016;139:138-47 pubmed publisher
    ..The myristoylation could thus have a structural role required in the folding/unfolding of NCS1 which is essential to its multiple biological functions. ..

More Information

Publications67

  1. He C, Masson J, Ramotar D. A Saccharomyces cerevisiae phleomycin-sensitive mutant, ph140, is defective in the RAD6 DNA repair gene. Can J Microbiol. 1996;42:1263-6 pubmed
    ..Moreover, a functional copy of the RAD6 gene restored full phleomycin resistance to strain ph140. Our findings indicate that the RAD6 protein is essential for yeast cellular resistance to phleomycin. ..
  2. Altaf M, Auger A, Monnet Saksouk J, Brodeur J, Piquet S, Cramet M, et al. NuA4-dependent acetylation of nucleosomal histones H4 and H2A directly stimulates incorporation of H2A.Z by the SWR1 complex. J Biol Chem. 2010;285:15966-77 pubmed publisher
    ..These results provide important mechanistic insight into the functional cross-talk between chromatin acetylation and ATP-dependent exchange of histone H2A variants. ..
  3. Orlicky S, Tran P, Sayre M, Edwards A. Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation. J Biol Chem. 2001;276:10097-102 pubmed
  4. Woolley G, Jaikaran A, Berezovski M, Calarco J, Krylov S, Smart O, et al. Reversible photocontrol of DNA binding by a designed GCN4-bZIP protein. Biochemistry. 2006;45:6075-84 pubmed
    ..It appears that conformational dynamics in the zipper domain make the transition state for isomerization readily available so that retention of reversible switching is observed. ..
  5. Zhang J, Smith K, Tackaberry T, Sun X, Carpenter P, Slugoski M, et al. Characterization of the transport mechanism and permeant binding profile of the uridine permease Fui1p of Saccharomyces cerevisiae. J Biol Chem. 2006;281:28210-21 pubmed
    ..This characterization of Fui1p contributes to the emerging knowledge of the structure and function of the Fur family of permeases, including the Fui1p orthologs of pathogenic fungi. ..
  6. Lacoste N, Utley R, Hunter J, Poirier G, Cote J. Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem. 2002;277:30421-4 pubmed
    ..In agreement with a role in regulating localization of histone deacetylase complexes like SIR, an increase of bulk histone acetylation is detected in dot1- cells. ..
  7. Nash P, Tang X, Orlicky S, Chen Q, Gertler F, Mendenhall M, et al. Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication. Nature. 2001;414:514-21 pubmed
    ..Multisite phosphorylation may be a more general mechanism to set thresholds in regulated protein-protein interactions. ..
  8. Yoshida E, Benkel B, Fong Y, Hickey D. Sequence and phylogenetic analysis of the SNF4/AMPK gamma subunit gene from Drosophila melanogaster. Genome. 1999;42:1077-87 pubmed
  9. Tennyson C, Lee J, Andrews B. A role for the Pcl9-Pho85 cyclin-cdk complex at the M/G1 boundary in Saccharomyces cerevisiae. Mol Microbiol. 1998;28:69-79 pubmed
    ..Our results show that PCL9 and PHO85 form a functional kinase complex and suggest a role for Pho85 CDKs at the M/G1 boundary. ..
  10. Jorgensen P, Nelson B, Robinson M, Chen Y, Andrews B, Tyers M, et al. High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants. Genetics. 2002;162:1091-9 pubmed
    ..In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach. ..
  11. Xu M, Soloveychik M, Ranger M, Schertzberg M, Shah Z, Raisner R, et al. Timing of transcriptional quiescence during gametogenesis is controlled by global histone H3K4 demethylation. Dev Cell. 2012;23:1059-71 pubmed publisher
  12. Guintini L, Tremblay M, Toussaint M, D Amours A, Wellinger R, Wellinger R, et al. Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction. Nucleic Acids Res. 2017;45:4577-4589 pubmed publisher
    ..The telomere terminal complex also prevents NER, however, this effect is largely dependent on the yKu-Sir4 interaction, but Sir2 and Sir3 independent. ..
  13. Duncan A, Ozawa T, Suzuki H, Rozen R. Assignment of the gene for the cytochrome c1 subunit of the mitochondrial cytochrome bc1 complex (CYC1) to human chromosome 8q24.3. Genomics. 1994;19:400-1 pubmed
  14. Chan J, Poon B, Salvi J, Olsen J, Emili A, Mekhail K. Perinuclear cohibin complexes maintain replicative life span via roles at distinct silent chromatin domains. Dev Cell. 2011;20:867-79 pubmed publisher
    ..Our work uncovers roles for Cohibin complexes and reveals relationships between nuclear compartmentalization, chromosome stability, and aging...
  15. Andrews B, Measday V. The cyclin family of budding yeast: abundant use of a good idea. Trends Genet. 1998;14:66-72 pubmed
    ..The cyclin family of budding yeast is reviewed from a functional perspective with an emphasis on what genetic and biochemical experiments have revealed about cyclin-CDK substrates. ..
  16. Imbeault D, Gamar L, Rufiange A, Paquet E, Nourani A. The Rtt106 histone chaperone is functionally linked to transcription elongation and is involved in the regulation of spurious transcription from cryptic promoters in yeast. J Biol Chem. 2008;283:27350-4 pubmed publisher
    ..Taken together, our results indicate a direct link for Rtt106 with transcription elongation and the chromatin dynamics associated with RNA polymerase II passage. ..
  17. Vickers M, Yao S, Baldwin S, Young J, Cass C. Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration of a transporter (FUI1) with high uridine selectivity in plasma membranes and a transporter (FUN26) with broad nucleoside selectivity in intracellular membranes. J Biol Chem. 2000;275:25931-8 pubmed
  18. Cote J, Peterson C, Workman J. Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding. Proc Natl Acad Sci U S A. 1998;95:4947-52 pubmed
    ..These results indicate that SWI/SNF can act transiently in the remodeling of chromatin structure, even before interactions of transcription factors. ..
  19. Fillingham J, Kainth P, Lambert J, van Bakel H, Tsui K, Pena Castillo L, et al. Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. Mol Cell. 2009;35:340-51 pubmed publisher
    ..Our data suggest that this pathway may represent a chromatin regulatory mechanism that is broadly used across the genome. ..
  20. Ketela T, Brown J, Stewart R, Bussey H. Yeast Skn7p activity is modulated by the Sln1p-Ypd1p osmosensor and contributes to regulation of the HOG pathway. Mol Gen Genet. 1998;259:372-8 pubmed
    ..The contribution of Skn7p to HOG pathway regulation appears to be modulated by the receiver domain, since non-phosphorylatable Skn7pD427N is unable to fully restore growth to ptc1/skn7 cells. ..
  21. Breitkreutz A, Boucher L, Tyers M. MAPK specificity in the yeast pheromone response independent of transcriptional activation. Curr Biol. 2001;11:1266-71 pubmed
    ..MAPK specificity in the pheromone response evidently occurs primarily at the substrate level, as opposed to specific kinase activation by dedicated signaling complexes. ..
  22. Marcoux N, Cloutier S, Zakrzewska E, Charest P, Bourbonnais Y, Pallotta D. Suppression of the profilin-deficient phenotype by the RHO2 signaling pathway in Saccharomyces cerevisiae. Genetics. 2000;156:579-92 pubmed
    ..Since none of the suppressors, except Rho2p, can correct the phenotype of the pfy1-111/rho2Delta strain, we propose a model in which the suppressors act through the Rho2p signaling pathway to repolarize cortical actin patches. ..
  23. Shen Z, Paquin N, Forget A, Chartrand P. Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p. Mol Biol Cell. 2009;20:2265-75 pubmed publisher
    ..This study reveals that a direct coupling between localization and translation regulation factors in the nucleus is required for proper cytoplasmic localization of mRNAs. ..
  24. Auger A, Galarneau L, Altaf M, Nourani A, Doyon Y, Utley R, et al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol. 2008;28:2257-70 pubmed publisher
    ..Our results identified the key central subunit for the structure and functions of the NuA4 histone acetyltransferase complex and functionally linked this activity with the histone variant H2AZ from yeast to human cells. ..
  25. Boone C, Bussey H, Andrews B. Exploring genetic interactions and networks with yeast. Nat Rev Genet. 2007;8:437-49 pubmed
    ..A comparative understanding of genetic-interaction networks promises insights into some long-standing genetic problems, such as the nature of quantitative traits and the basis of complex inherited disease. ..
  26. Govindan M, Meng X, Denis C, Webb P, Baxter J, Walfish P. Identification of CCR4 and other essential thyroid hormone receptor co-activators by modified yeast synthetic genetic array analysis. Proc Natl Acad Sci U S A. 2009;106:19854-9 pubmed publisher
    ..These findings indicate that a modified yeast synthetic genetic array strategy is a feasible method for unbiased identification of conserved genes essential for TR and other nuclear receptor hormone functions in mammals. ..
  27. Kvas S, Gloor G, Brandl C. Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1. BMC Genet. 2012;13:19 pubmed publisher
  28. Li H, Page N, Bussey H. Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p. Yeast. 2002;19:1097-112 pubmed
    ..EM image analysis and beta-1,6-glucan localization indicated abnormal wall proliferation in the mother cells of these mutants. The pattern of cell wall hypertrophy indicates a failure to restrict cell wall growth to the bud. ..
  29. Dalal K, Duong F. The SecY complex: conducting the orchestra of protein translocation. Trends Cell Biol. 2011;21:506-14 pubmed publisher
    ..In this review, we cover the current state of the field including some of the newest and most exciting findings on channel structure and mechanism of action. ..
  30. Gsponer J, Babu M. Cellular strategies for regulating functional and nonfunctional protein aggregation. Cell Rep. 2012;2:1425-37 pubmed publisher
    ..This strategy may prevent formation of undesirable aggregates and keep functional assemblies/aggregates under control. ..
  31. Nourani A, Utley R, Allard S, Cote J. Recruitment of the NuA4 complex poises the PHO5 promoter for chromatin remodeling and activation. EMBO J. 2004;23:2597-607 pubmed
    ..These results indicate that, before induction, NuA4 complex recruitment by Pho2 is an essential event that presets the PHO5 promoter for subsequent binding by Pho4, chromatin remodeling and transcription. ..
  32. Doyon Y, Selleck W, Lane W, Tan S, Cote J. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Mol Cell Biol. 2004;24:1884-96 pubmed
    ..In conclusion, the NuA4 HAT complex is highly conserved in eukaryotes, in which it plays primary roles in transcription, cellular response to DNA damage, and cell cycle control. ..
  33. Rossetto D, Cramet M, Wang A, Steunou A, Lacoste N, Schulze J, et al. Eaf5/7/3 form a functionally independent NuA4 submodule linked to RNA polymerase II-coupled nucleosome recycling. EMBO J. 2014;33:1397-415 pubmed publisher
    ..Taken together, these results lead to a model where Eaf5/7/3 associates with elongating polymerase to promote the disruption of nucleosomes in its path, but also their refolding in its wake. ..
  34. Gauthier L, Dziak R, Kramer D, Leishman D, Song X, Ho J, et al. The role of the carboxyterminal domain of RNA polymerase II in regulating origins of DNA replication in Saccharomyces cerevisiae. Genetics. 2002;162:1117-29 pubmed
    ..Furthermore, we show that pol II is recruited to ARS1. We propose that in S. cerevisiae a mechanism of coordinating pol II transcription and DNA replication is mediated by the CTD of pol II. ..
  35. Rivera I, Shore G, Schleiff E. Cloning and characterization of a 35-kDa mouse mitochondrial outer membrane protein MOM35 with high homology to Tom40. J Bioenerg Biomembr. 2000;32:111-21 pubmed
    ..Subsequent investigation has revealed that this region interacts specifically in vitro with preproteins proposed to be imported by a Tom40-dependent pathway. ..
  36. Measday V, Moore L, Retnakaran R, Lee J, Donoviel M, Neiman A, et al. A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase. Mol Cell Biol. 1997;17:1212-23 pubmed
    ..Our studies suggest that Pho85 associates with multiple cyclins and that subsets of cyclins may direct Pho85 to perform distinct roles in cell growth and division. ..
  37. Ho Y, Mason S, Kobayashi R, Hoekstra M, Andrews B. Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:581-6 pubmed
  38. Lusson J, Vieau D, Hamelin J, Day R, Chretien M, Seidah N. cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a candidate proprotein convertase expressed in endocrine and nonendocrine cells. Proc Natl Acad Sci U S A. 1993;90:6691-5 pubmed
    ..In situ hybridization of rat brain sections demonstrated a unique distribution of PC5 compared to PC1, PC2, and furin. ..
  39. Horowitz A, Lapointe J, Eid R, Sheibani S, Gharib N, Jones N, et al. The human septin7 and the yeast CDC10 septin prevent Bax and copper mediated cell death in yeast. Biochim Biophys Acta. 2013;1833:3186-3194 pubmed publisher
    ..Taken together, our analysis suggests that anti-apoptosis is a novel yet evolutionarily conserved property of the septin7 sub-family of septins. ..
  40. Chang E, Novoa C, Aristizabal M, Coulombe Y, Segovia R, Chaturvedi R, et al. RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability. J Cell Biol. 2017;216:3991-4005 pubmed publisher
    ..Together, our data describe a conserved role for Sgs1/BLM in R-loop suppression and support an increasingly broad view of DNA repair and replication fork stabilizing proteins as modulators of R-loop-mediated genome instability. ..
  41. Fang N, Chan G, Zhu M, Comyn S, Persaud A, Deshaies R, et al. Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat Cell Biol. 2014;16:1227-37 pubmed publisher
    ..Our results support a bipartite recognition mechanism combining direct and chaperone-dependent ubiquitylation of misfolded cytosolic proteins by Rsp5. ..
  42. Kennedy E, Dysart M, Lianga N, Williams E, Pilon S, Doré C, et al. Redundant Regulation of Cdk1 Tyrosine Dephosphorylation in Saccharomyces cerevisiae. Genetics. 2016;202:903-10 pubmed publisher
  43. Andrews B, Moore L. Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro. Proc Natl Acad Sci U S A. 1992;89:11852-6 pubmed
  44. Malleshaiah M, Shahrezaei V, Swain P, Michnick S. The scaffold protein Ste5 directly controls a switch-like mating decision in yeast. Nature. 2010;465:101-5 pubmed publisher
    ..Similar mechanisms may govern cellular decisions in higher organisms and be disrupted in cancer. ..
  45. Lambert A, Perron M, Lavoie E, Pallotta D. The Saccharomyces cerevisiae Arf3 protein is involved in actin cable and cortical patch formation. FEMS Yeast Res. 2007;7:782-95 pubmed
    ..Both double mutant strains have actin cytoskeleton defects. Our results support a role for ARF3 in cell polarity and the organization of the actin cytoskeleton. ..
  46. Savchenko A, Krogan N, Cort J, Evdokimova E, Lew J, Yee A, et al. The Shwachman-Bodian-Diamond syndrome protein family is involved in RNA metabolism. J Biol Chem. 2005;280:19213-20 pubmed publisher
    ..Our observations, taken together with previous reports, support the conclusion that SBDS and its homologues play a role in RNA metabolism...
  47. Nourani A, Howe L, Pray Grant M, Workman J, Grant P, Cote J. Opposite role of yeast ING family members in p53-dependent transcriptional activation. J Biol Chem. 2003;278:19171-5 pubmed
    ..They also demonstrate the key specific role of ING proteins in different chromatin modifying complexes and their opposite functions in p53-dependent transcription. ..
  48. Nourani A, Doyon Y, Utley R, Allard S, Lane W, Cote J. Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex. Mol Cell Biol. 2001;21:7629-40 pubmed
    ..They also suggest that regulation of mammalian cell proliferation by p53-dependent transcriptional activation functions through recruitment of an ING1-containing histone acetyltransferase complex. ..
  49. Bouganim N, David J, Wysocki R, Ramotar D. Yap1 overproduction restores arsenite resistance to the ABC transporter deficient mutant ycf1 by activating ACR3 expression. Biochem Cell Biol. 2001;79:441-8 pubmed
    ..We conclude that Yap1 may compete with Yap8 for binding to the ACR3 promoter, but is unable to act as a potent activator...
  50. Galarneau L, Nourani A, Boudreault A, Zhang Y, Heliot L, Allard S, et al. Multiple links between the NuA4 histone acetyltransferase complex and epigenetic control of transcription. Mol Cell. 2000;5:927-37 pubmed
    ..These data along with the known epigenetic roles of Act3/Arp4 and homologs of Epl1 and Esa1 strongly support an essential role for chromatin structure modification by NuA4 in transcription regulation in vivo. ..
  51. Saleh A, Schieltz D, Ting N, McMahon S, Litchfield D, Yates J, et al. Tra1p is a component of the yeast Ada.Spt transcriptional regulatory complexes. J Biol Chem. 1998;273:26559-65 pubmed
    ..Despite the similarity of Tra1p to a group of putative protein kinases, we have not detected protein kinase activity within immunoprecipitates of Tra1p or the Ada.Spt complexes. ..
  52. Udell C, Lee S, Davey S. HRAD1 and MRAD1 encode mammalian homologues of the fission yeast rad1(+) cell cycle checkpoint control gene. Nucleic Acids Res. 1998;26:3971-6 pubmed
    ..The ability of HRAD1 to partially complement yeast rad1 mutants suggests that this gene is required for G2checkpoint control in human cells. ..
  53. Seidah N, Hamelin J, Mamarbachi M, Dong W, Tardos H, Mbikay M, et al. cDNA structure, tissue distribution, and chromosomal localization of rat PC7, a novel mammalian proprotein convertase closest to yeast kexin-like proteinases. Proc Natl Acad Sci U S A. 1996;93:3388-93 pubmed
    ..The gene for PC7 (Pcsk7) was mapped to mouse chromosome 9 by linkage analysis of an interspecific backcross DNA panel. ..
  54. Auclair D, Lang B, Forest P, DesGroseillers L. Analysis of genes encoding highly conserved lysine-rich proteins in Aplysia californica and Saccharomyces cerevisiae. Eur J Biochem. 1994;220:997-1003 pubmed
    ..Northern blotting experiments revealed that the expression of the gene is strongly repressed at 39 degrees C. ..
  55. Seidah N, Chretien M, Day R. The family of subtilisin/kexin like pro-protein and pro-hormone convertases: divergent or shared functions. Biochimie. 1994;76:197-209 pubmed
    ..Although the genetic organization of the convertase genes is very similar, they exhibit unique promoter sequences and only furin and PACE4 genes are localized on the same chromosome. ..
  56. Kapoor P, Lavoie B, Frappier L. EBP2 plays a key role in Epstein-Barr virus mitotic segregation and is regulated by aurora family kinases. Mol Cell Biol. 2005;25:4934-45 pubmed
  57. Zakrzewska E, Perron M, Laroche A, Pallotta D. A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae. Genetics. 2003;165:985-95 pubmed
    ..In this work, we demonstrate a role for Gea1p, Gea2p, and Arf3p in the organization of the actin cytoskeleton. ..
  58. Steiner Mosonyi M, Leslie D, Dehghani H, Aitchison J, Mangroo D. Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae. J Biol Chem. 2003;278:32236-45 pubmed
    ..Taken together, the results suggest that Utp8p is an essential intranuclear component of the nuclear tRNA export machinery, which may channel tRNA to the various tRNA export pathways operating in S. cerevisiae. ..