Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Belgium
Topic: saccharomyces cerevisiae proteins
Publications102 found, 100 shown here
- Abdel Sater F, El Bakkoury M, Urrestarazu A, Vissers S, Andre B. Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Mol Cell Biol. 2004;24:9771-85 pubmed..Upon detection of external amino acids, Ssy1-Ptr3 likely allows-in a manner dependent on SCF(Grr1)-the Ssy5 endoprotease to gain access to and to cleave Stp1, this requiring prior phosphorylation of Stp1 by casein kinase I. ..
- Hertveldt K, Robben J, Volckaert G. Whole genome phage display selects for proline-rich Boi polypeptides against Bem1p. Biotechnol Lett. 2006;28:1233-9 pubmed..7 x 10(7) independent clones of sheared S. cerevisiae genomic DNA fused to a truncated M13 gene III. This study corroborates the value of fragmented-proteome display to identify strong and direct interacting protein modules. ..
- Fayruzov T, Janssen J, Vermeir D, Cornelis C, De Cock M. Modelling gene and protein regulatory networks with answer set programming. Int J Data Min Bioinform. 2011;5:209-29 pubmed..The obtained steady states of these networks correspond to those of the Boolean networks. ..
- Vandenbol M, Jauniaux J, Grenson M. The Saccharomyces cerevisiae NPR1 gene required for the activity of ammonia-sensitive amino acid permeases encodes a protein kinase homologue. Mol Gen Genet. 1990;222:393-9 pubmed..Hence, the NPR1 protein probably acts at the post-transcriptional level. Proteins that may serve as substrates for phosphorylation are discussed. ..
- De Veylder L, Segers G, Glab N, Casteels P, Van Montagu M, Inze D. The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt. FEBS Lett. 1997;412:446-52 pubmed..Cks1At binds both Cdc2aAt and Cdc2bAt in vivo and in vitro. Furthermore, we demonstrate that the fission yeast Suc1 binds Cdc2aAt but only weakly Cdc2bAt, whereas the human CksHs1 associated exclusively with Cdc2aAt. ..
- Hertveldt K, Dechassa M, Robben J, Volckaert G. Identification of Gal80p-interacting proteins by Saccharomyces cerevisiae whole genome phage display. Gene. 2003;307:141-9 pubmed..Interactions were confirmed by ELISA. These results demonstrate the accessibility of the S. cerevisiae genome to display technology for protein-protein interaction screening. ..
- Feller A, Georis I, Tate J, Cooper T, Dubois E. Alterations in the Ure2 ?Cap domain elicit different GATA factor responses to rapamycin treatment and nitrogen limitation. J Biol Chem. 2013;288:1841-55 pubmed publisher..This in turn is more consistent with the existence of distinct pathways for TorC1- and nitrogen limitation-dependent control than it is with these stimuli representing sequential steps in a single regulatory pathway. ..
- Van Hoof C, Martens E, Longin S, Jordens J, Stevens I, Janssens V, et al. Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2. Biochem J. 2005;386:93-102 pubmed..Ypa1 as well as Ypa2 can reactivate all these inactive complexes, except Pph22-Yme. Ypa1 is the most potent activator of PP2A activity, suggesting that there is no direct correlation between activation potential and binding capacity. ..
- Lemaire S, Glupczynski Y, Duval V, Joris B, Tulkens P, Van Bambeke F. Activities of ceftobiprole and other cephalosporins against extracellular and intracellular (THP-1 macrophages and keratinocytes) forms of methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2009;53:2289-97 pubmed publisher
- Castermans D, Somers I, Kriel J, Louwet W, Wera S, Versele M, et al. Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast. Cell Res. 2012;22:1058-77 pubmed publisher..Our results provide novel insight into the complex regulatory role of these two major protein phosphatases in glucose regulation. ..
- Talibi D, Grenson M, Andre B. Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res. 1995;23:550-7 pubmed..We propose that tight coupling between the UASGABA and UASGATA elements enables the cell to integrate, according to its nitrogen status, the induced expression levels of UGA4. ..
- Van Hoof C, Janssens V, Dinishiotu A, Merlevede W, Goris J. Functional analysis of conserved domains in the phosphotyrosyl phosphatase activator. Molecular cloning of the homologues from Drosophila melanogaster and Saccharomyces cerevisiae. Biochemistry. 1998;37:12899-908 pubmed..Moreover, we observed that PTPA truncated at glycine266 behaves as a dominant negative mutant since it is inhibitory to the wild-type PTPA. ..
- Lauwers E, Andre B. Association of yeast transporters with detergent-resistant membranes correlates with their cell-surface location. Traffic. 2006;7:1045-59 pubmed..We discuss a model where yeast plasma membrane proteins are systematically associated with sphingolipid/ergosterol-enriched microdomains when located at the cell surface. ..
- Aerts A, Zabrocki P, Francois I, Carmona Gutierrez D, Govaert G, Mao C, et al. Ydc1p ceramidase triggers organelle fragmentation, apoptosis and accelerated ageing in yeast. Cell Mol Life Sci. 2008;65:1933-42 pubmed publisher..In conclusion, this study describes a direct link between ceramide metabolism in yeast and mitochondrial and vacuolar fragmentation and function, with consequences for chronological lifespan in yeast. ..
- Andre B, Talibi D, Soussi Boudekou S, Hein C, Vissers S, Coornaert D. Two mutually exclusive regulatory systems inhibit UASGATA, a cluster of 5'-GAT(A/T)A-3' upstream from the UGA4 gene of Saccharomyces cerevisiae. Nucleic Acids Res. 1995;23:558-64 pubmed..The role of UASGATA in the expression of the UGA4 gene is discussed in relation to its sensitivity to the two distinct repression systems. ..
- Vranová E, Tähtiharju S, Sriprang R, Willekens H, Heino P, Palva E, et al. The AKT3 potassium channel protein interacts with the AtPP2CA protein phosphatase 2C. J Exp Bot. 2001;52:181-2 pubmed..A deletion analysis indicated that the catalytic domain of AtPP2CA was essential for the interaction with AKT3. Furthermore, the related PP2C phosphatase ABI1 did not interact with AKT3 in yeast. ..
- Rep M, Proft M, Remize F, Tamas M, Serrano R, Thevelein J, et al. The Saccharomyces cerevisiae Sko1p transcription factor mediates HOG pathway-dependent osmotic regulation of a set of genes encoding enzymes implicated in protection from oxidative damage. Mol Microbiol. 2001;40:1067-83 pubmed..The five Sko1p target genes described here are suitable models for studying the interplay between osmotic and oxidative responses at the molecular and physiological levels. ..
- Velasco I, Tenreiro S, Calderon I, Andre B. Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids. Eukaryot Cell. 2004;3:1492-503 pubmed..We propose that Aqr1 catalyzes transport of excess amino acids into vesicles, which then release them in the extracellular space by exocytosis. ..
- Brohée S, van Helden J. Evaluation of clustering algorithms for protein-protein interaction networks. BMC Bioinformatics. 2006;7:488 pubmed..The other two algorithms are clearly weaker under most conditions. The analysis of high-throughput data supports the superiority of MCL for the extraction of complexes from interaction networks. ..
- Leporé N, Lafontaine D. A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast. PLoS ONE. 2011;6:e24962 pubmed publisher..Altogether these results indicate that Nrd1-Nab3 contributes to recruiting the nucleolar surveillance to elongating polymerases to survey nascent rRNA transcripts...
- D Halluin K, Vanderstraeten C, Stals E, Cornelissen M, Ruiter R. Homologous recombination: a basis for targeted genome optimization in crop species such as maize. Plant Biotechnol J. 2008;6:93-102 pubmed
- Georis I, Feller A, Vierendeels F, Dubois E. The yeast GATA factor Gat1 occupies a central position in nitrogen catabolite repression-sensitive gene activation. Mol Cell Biol. 2009;29:3803-15 pubmed publisher..Dal80-dependent repression of NCR-sensitive gene expression occurred at three possible levels: Dal80 represses GAT1 expression, it competes with Gat1 for binding, and it directly represses NCR gene transcription. ..
- Crabeel M, Soetens O, De Rijcke M, Pratiwi R, Pankiewicz R. The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis. J Biol Chem. 1996;271:25011-8 pubmed..Arg11p thus appears to fulfill a partially redundant function requiring its 27 carboxyl-terminal amino acids. As a working hypothesis, we propose that Arg11p participates in the export of matrix-made ornithine into the cytosol. ..
- Soussi Boudekou S, Vissers S, Urrestarazu A, Jauniaux J, Andre B. Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol. 1997;23:1157-68 pubmed..In addition, both Uga43p and Gzf3p moderately regulate expression of their own genes. These two proteins seem to be parts of a complex network of GATA factors which probably play a determining role in nitrogen-regulated transcription. ..
- Bizimungu C, De Neve N, Burny A, Bach S, Bontemps F, Portetelle D, et al. Expression in a RabGAP yeast mutant of two human homologues, one of which is an oncogene. Biochem Biophys Res Commun. 2003;310:498-504 pubmed..As two highly conserved amino acids, including the catalytic arginine, are mutated in the oncTre210p TBC domain, we restored these two amino acids and expressed the modified Tre2 cDNA in the yeast mutant. ..
- Abdel Sater F, Jean C, Merhi A, Vissers S, Andre B. Amino acid signaling in yeast: activation of Ssy5 protease is associated with its phosphorylation-induced ubiquitylation. J Biol Chem. 2011;286:12006-15 pubmed publisher..We propose that the Ssy5 protease is activated by phosphorylation-induced ubiquitylation, the effect of which is relief from inhibition by its prodomain. ..
- Lauwers E, Grossmann G, Andre B. Evidence for coupled biogenesis of yeast Gap1 permease and sphingolipids: essential role in transport activity and normal control by ubiquitination. Mol Biol Cell. 2007;18:3068-80 pubmed..We propose that coupled biogenesis of Gap1 and SLs would create an SL microenvironment essential to the normal conformation, function, and control of ubiquitination of the permease. ..
- Babiychuk E, Kushnir S, Belles Boix E, Van Montagu M, Inze D. Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. J Biol Chem. 1995;270:26224-31 pubmed..The data suggested that proteins encoded by the isolated cDNAs play a distinct role in plant antioxidant defense and are possibly involved in NAD(P)/NAD(P)H homeostasis...
- Hein C, Springael J, Volland C, Haguenauer Tsapis R, Andre B. NPl1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase. Mol Microbiol. 1995;18:77-87 pubmed..Our results show that the Npi1/Rsp5 ubiquitin-protein ligase participates in induced degradation of at least two permeases, Gap1p and Fur4p, and probably also other proteins. ..
- Parker A, Van de Weyer I, Laus M, Oostveen I, Yon J, Verhasselt P, et al. A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease. J Biol Chem. 1998;273:18332-9 pubmed..The biological functions of the two proteins remain to be determined. ..
- Iraqui I, Vissers S, Bernard F, De Craene J, Boles E, Urrestarazu A, et al. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol Cell Biol. 1999;19:989-1001 pubmed..The essential role of Grr1p in this amino acid signaling pathway lends further support to the hypothesis that this protein participates in integrating nutrient availability with the cell cycle. ..
- Nikko E, Marini A, Andre B. Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway. J Biol Chem. 2003;278:50732-43 pubmed..This recycling seems to be accompanied by deubiquitination of the permease and unexpectedly requires a normal endosome-to-vacuole transport function. ..
- Hoang T, Peng W, Vanrobays E, Krogan N, Hiley S, Beyer A, et al. Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction. Mol Cell Biol. 2005;25:5523-34 pubmed..The identification of ABT1 in a large-scale analysis of the human nucleolar proteome indicates that its role may also be conserved in mammals. ..
- Georis I, Tate J, Feller A, Cooper T, Dubois E. Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeast. Mol Cell Biol. 2011;31:92-104 pubmed publisher..e., ure2? is epistatic to pph21? pph22?. This final observation adds additional support to our previous conclusion that the Gln3 and Gat1 GATA factor localizations are predominantly controlled by different regulatory pathways. ..
- Qiu H, Dubois E, Broen P, Messenguy F. Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Gen Genet. 1990;222:192-200 pubmed..Gene disruption of the ARGRIII gene impairs the growth of the mutant on rich medium, showing that ARGRIII has a pleiotropic role in the cell. ..
- Feller A, Dubois E, Ramos F, Pierard A. Repression of the genes for lysine biosynthesis in Saccharomyces cerevisiae is caused by limitation of Lys14-dependent transcriptional activation. Mol Cell Biol. 1994;14:6411-8 pubmed..Such findings suggest that activation by alpha-aminoadipate semialdehyde and the apparent repression by lysine are related mechanisms. Lysine possibly acts by limiting the supply of the coinducer, alpha-aminoadipate semialdehyde. ..
- Andre B, Hein C, Grenson M, Jauniaux J. Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. Mol Gen Genet. 1993;237:17-25 pubmed..In the absence of the pleiotropic UGA43/DAL80 repressor, UGA4 is constitutively expressed at high level. ..
- Matthijs G, Schollen E, Pardon E, Veiga da Cunha M, Jaeken J, Cassiman J, et al. Mutations in PMM2, a phosphomannomutase gene on chromosome 16p13, in carbohydrate-deficient glycoprotein type I syndrome (Jaeken syndrome). Nat Genet. 1997;16:88-92 pubmed..Our results give conclusive support to the biochemical finding that the phosphomannomutase deficiency is the basis for CDG1. ..
- De Craene J, Soetens O, Andre B. The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease. J Biol Chem. 2001;276:43939-48 pubmed..Npr1 belongs to a subgroup of protein kinases, some of which are reported to exert a positive control on the activity of other permeases. We propose that these kinases also function as regulators of permease trafficking. ..
- El Alami M, Feller A, Piérard A, Dubois E. The proper folding of a long C-terminal segment of the yeast Lys14p regulator is required for activation of LYS genes in response to the metabolic effector. Mol Microbiol. 2002;43:1629-39 pubmed..Our results suggest that the proper folding of the two-thirds C-terminal portion of Lys14p is essential not only to activate transcription but also to modulate it according to alphaAASA concentration. ..
- Decottignies A, Evain A, Ghislain M. Binding of Cdc48p to a ubiquitin-related UBX domain from novel yeast proteins involved in intracellular proteolysis and sporulation. Yeast. 2004;21:127-39 pubmed..Together, these results indicate that the Cuil-3 proteins form complexes that are components of the ubiquitin-proteasome system. ..
- Colau G, Thiry M, Leduc V, Bordonné R, Lafontaine D. The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology. Mol Cell Biol. 2004;24:7976-86 pubmed..This conclusion is compatible with a model of self-organization for nucleolar assembly. ..
- Swinnen E, Rosseels J, Winderickx J. The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. Curr Genet. 2005;48:18-33 pubmed
- de Thozée C, Cronin S, Goj A, Golin J, Ghislain M. Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding fold. Mol Microbiol. 2007;63:811-25 pubmed..These results suggest that Pdr5p requires a properly folded nucleotide-binding domain for trafficking to the plasma membrane...
- Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi T, Maere S, et al. The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. Plant Cell. 2007;19:417-32 pubmed..Based on the mutant characterization, we postulate that HUB1 mediates gene activation and cell cycle regulation probably through chromatin modifications. ..
- Boeckstaens M, Andre B, Marini A. The yeast ammonium transport protein Mep2 and its positive regulator, the Npr1 kinase, play an important role in normal and pseudohyphal growth on various nitrogen media through retrieval of excreted ammonium. Mol Microbiol. 2007;64:534-46 pubmed..Finally, we show that Mep2 participates, through ammonium leakage compensation, in pseudohyphal growth induced by amino acid starvation. This argues further in favour of tight coupling of Mep2 transport and sensor functions. ..
- Thevissen K, Yen W, Carmona Gutierrez D, Idkowiak Baldys J, Aerts A, Francois I, et al. Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;303:163-8 pubmed publisher..These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process. ..
- Popova Y, Thayumanavan P, Lonati E, Agrochão M, Thevelein J. Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor. Proc Natl Acad Sci U S A. 2010;107:2890-5 pubmed publisher..Our results provide to the best of our knowledge the first insight into the molecular mechanism of a phosphate transceptor. ..
- Kolaczkowski M, van der Rest M, Cybularz Kolaczkowska A, Soumillion J, Konings W, Goffeau A. Anticancer drugs, ionophoric peptides, and steroids as substrates of the yeast multidrug transporter Pdr5p. J Biol Chem. 1996;271:31543-8 pubmed..Our experimental system opens new possibilities for the analysis of structure-function relationship of multidrug transporter substrates and inhibitors...
- Voet M, Defoor E, Verhasselt P, Riles L, Robben J, Volckaert G. The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast. 1997;13:177-82 pubmed..YGL144c has a serine active site signature of lipases. YGL141w has limited homology to several human proteins, one of which mediates complex formation between papillomavirus E6 oncoprotein and tumor suppressor protein p53. ..
- Vanden Berghe W, De Bosscher K, Boone E, Plaisance S, Haegeman G. The nuclear factor-kappaB engages CBP/p300 and histone acetyltransferase activity for transcriptional activation of the interleukin-6 gene promoter. J Biol Chem. 1999;274:32091-8 pubmed..We conclude that, within an "enhanceosome-like" structure, NF-kappaB is the central mediator of TNF-induced IL-6 gene expression, involving CBP/p300 and requiring histone acetyltransferase activity. ..
- Belles Boix E, Babiychuk E, Montagu M, Inze D, Kushnir S. CEF, a sec24 homologue of Arabidopsis thaliana, enhances the survival of yeast under oxidative stress conditions. J Exp Bot. 2000;51:1761-2 pubmed..CEF is homologous to animal and yeast Sec24 proteins. These data suggest that CEF plays a protective role through protein transport during growth under pro-oxidant conditions. ..
- Marini A, Matassi G, Raynal V, Andre B, Cartron J, Cherif Zahar B. The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet. 2000;26:341-4 pubmed..Our results provide the first experimental evidence for a direct role of RhAG and RhGK in ammonium transport. These findings are of high interest, because no specific ammonium transport system has been characterized so far in human. ..
- Boudolf V, Rombauts S, Naudts M, Inze D, De Veylder L. Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis. J Exp Bot. 2001;52:1381-2 pubmed..Differences in the expression patterns in roots, leaves and stems suggest unique roles for each CDK. ..
- Mehdi K, Thierie J, Penninckx M. gamma-Glutamyl transpeptidase in the yeast Saccharomyces cerevisiae and its role in the vacuolar transport and metabolism of glutathione. Biochem J. 2001;359:631-7 pubmed..Yeast appears to be a useful model for studying gamma-GT physiology and GSH metabolism...
- Van Mullem V, Landrieux E, Vandenhaute J, Thuriaux P. Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol. 2002;43:1105-13 pubmed..Thus, the N-terminal zinc domain of Rpa12p determines its anchoring to RNA polymerase I and is the only critical part of that subunit in vivo. ..
- El Alami M, Messenguy F, Scherens B, Dubois E. Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol. 2003;49:457-68 pubmed..Our results indicate that Arg82p is a bifunctional protein whose inositol kinase activity plays a role in multiple signalling cascades, and whose acidic domain protects two MADS-box proteins against degradation. ..
- Verstrepen K, Van Laere S, Vanderhaegen B, Derdelinckx G, Dufour J, Pretorius I, et al. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003;69:5228-37 pubmed..Interestingly, overexpression of different alleles of ATF1 and ATF2 led to different ester production rates, indicating that differences in the aroma profiles of yeast strains may be partially due to mutations in their ATF genes. ..
- Abdel Sater F, Iraqui I, Urrestarazu A, Andre B. The external amino acid signaling pathway promotes activation of Stp1 and Uga35/Dal81 transcription factors for induction of the AGP1 gene in Saccharomyces cerevisiae. Genetics. 2004;166:1727-39 pubmed..Finally, the data show that the TOR pathway mediating global nitrogen control of transcription does not interfere with the external amino acid signaling pathway. ..
- Thevissen K, Idkowiak Baldys J, Im Y, Takemoto J, Francois I, Ferket K, et al. SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis. FEBS Lett. 2005;579:1973-7 pubmed..These results show that SKN1, together with IPT1, is involved in sphingolipid biosynthesis in S. cerevisiae. ..
- Steuve S, Devosse T, Lauwers E, Vanderwinden J, Andre B, Courtoy P, et al. Rhophilin-2 is targeted to late-endosomal structures of the vesicular machinery in the presence of activated RhoB. Exp Cell Res. 2006;312:3981-9 pubmed..As for its yeast homologue, Npi3/Bro1p, the Bro1 domain of rhophilin-2 is necessary to its recruitment to the vesicular structures, which are not labeled for EEA1 nor Lamp1, but well with the late endosome marker CD63. ..
- Nikko E, Andre B. Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway. Traffic. 2007;8:566-81 pubmed..We propose that deubiquitination by Doa4 of cargo proteins and/or some components of the MVB sorting machinery is essential to correct sorting of cargoes into the MVB pathway. ..
- Pokrzywa W, Guerriat B, Dodzian J, Morsomme P. Dual sorting of the Saccharomyces cerevisiae vacuolar protein Sna4p. Eukaryot Cell. 2009;8:278-86 pubmed publisher..The presence of these two motifs allows Sna4p to localize to both the vacuolar membrane and the lumen. ..
- Schillewaert S, Wacheul L, Lhommé F, Lafontaine D. The evolutionarily conserved protein Las1 is required for pre-rRNA processing at both ends of ITS2. Mol Cell Biol. 2012;32:430-44 pubmed publisher..Consistently, we note the conspicuous presence of ~7- or 8-nucleotide extensions on both ends of 5.8S rRNA precursors and at the 5' end of pre-25S RNAs suggestive of a protected spacer fragment of similar length. ..
- Sharma S, Yang J, van Nues R, Watzinger P, Kötter P, Lafontaine D, et al. Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation. PLoS Genet. 2017;13:e1006804 pubmed publisher..Compared to yeast, the number of orphan box C/D snoRNAs in higher eukaryotes is much larger and we hypothesize that several of these may be involved in base-modifications. ..
- Decottignies A, Grant A, Nichols J, de Wet H, McIntosh D, Goffeau A. ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p. J Biol Chem. 1998;273:12612-22 pubmed..Both ABC transporters exhibit ATP hydrolysis in vitro, but Pdr5p ATPase activity is about 15 times higher than that of Yor1p, which may indicate mechanistic or regulatory differences between the two enzymes...
- Springael J, Andre B. Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1253-63 pubmed..We show here that mutant Gap1 permeases affected in these sequences still bind Ub. Furthermore, we provide evidence that only a small fraction of Gap1 is modified by Ub after addition of NH4+ to mutants defective in endocytosis. ..
- Amar N, Messenguy F, El Bakkoury M, Dubois E. ArgRII, a component of the ArgR-Mcm1 complex involved in the control of arginine metabolism in Saccharomyces cerevisiae, is the sensor of arginine. Mol Cell Biol. 2000;20:2087-97 pubmed
- Thevissen K, Cammue B, Lemaire K, Winderickx J, Dickson R, Lester R, et al. A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii). Proc Natl Acad Sci U S A. 2000;97:9531-6 pubmed..Our data support a model in which membrane patches containing sphingolipids act as binding sites for DmAMP1 or, alternatively, are required to anchor membrane or cell wall-associated proteins, which themselves interact with DmAMP1. ..
- Hertveldt K, Robben J, Volckaert G. In vivo selectively infective phage as a tool to detect protein interactions: evaluation of a novel vector system with yeast Ste7p-Fus3p interacting proteins. Yeast. 2002;19:499-508 pubmed..The presence of the interacting N1-Fus3p adapter increased the infectivity of Ste7p-N2-CT phages approximately 1400-fold, which makes SIP a promising technology for the detection and further investigation of interacting proteins. ..
- Springael J, Nikko E, Andre B, Marini A. Yeast Npi3/Bro1 is involved in ubiquitin-dependent control of permease trafficking. FEBS Lett. 2002;517:103-9 pubmed..We propose that proteins of the Npi3/Bro1 family, including homologues from higher species, may play a conserved role in ubiquitin-dependent control of membrane protein trafficking. ..
- Desmyter L, Verstraelen J, Dewaele S, Libert C, Contreras R, Chen C. Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae. Biogerontology. 2007;8:527-35 pubmed..We therefore demonstrate that a nonclassical export pathway functions as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient. ..
- Nikko E, Andre B. Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. Eukaryot Cell. 2007;6:1266-77 pubmed..Our data indicate that Bro1 interacts with several ESCRT components and contributes via its PRD to associating ubiquitinating and deubiquitinating enzymes with the MVB sorting machinery. ..
- Renard H, Demaegd D, Guerriat B, Morsomme P. Efficient ER exit and vacuole targeting of yeast Sna2p require two tyrosine-based sorting motifs. Traffic. 2010;11:931-46 pubmed publisher..Sna2p is the first yeast protein in which two YXXØ motifs have been identified and both were shown to be functional at two different steps of the secretory pathway, ER exit and Golgi-to-vacuole transport. ..
- Georis I, Tate J, Cooper T, Dubois E. Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine. J Biol Chem. 2011;286:44897-912 pubmed publisher..Collectively, our data support the existence of two different nitrogen-responsive regulatory pathways, one inhibited by Msx and the other by rapamycin. ..
- Kimpe M, Voordeckers K, Thevelein J, Van Zeebroeck G. Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2? system. Biochem Biophys Res Commun. 2012;419:89-94 pubmed publisher..Hence, the physiological importance of the close interactions between Pkh1 and Gcn2 or eIF2 could depend on other conditions and/or other targets of the Gcn2/eIF2 system. ..
- Wilms T, Swinnen E, Eskes E, Dolz Edo L, Uwineza A, Van Essche R, et al. The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability. PLoS Genet. 2017;13:e1006835 pubmed publisher..As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing. ..
- Van Huffel C, Dubois E, Messenguy F. Cloning and sequencing of Schizosaccharomyces pombe car1 gene encoding arginase. Expression of the arginine anabolic and catabolic genes in response to arginine and related metabolites. Yeast. 1994;10:923-33 pubmed..cerevisiae, and also indicates that in S. pombe enzyme compartmentation is not always sufficient to control the arginine metabolic flux. ..
- van Dyck L, Pearce D, Sherman F. PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae. J Biol Chem. 1994;269:238-42 pubmed..PIM1 mRNA is constitutively expressed but is increased after thermal stress, suggesting that Pim1 may play a role in the heat shock response. ..
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