Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in United States
Locale: United States
Topic: saccharomyces cerevisiae
Publications772 found, 100 shown here
- Howard S, Hester A, Herman P. The Ras/PKA signaling pathway may control RNA polymerase II elongation via the Spt4p/Spt5p complex in Saccharomyces cerevisiae. Genetics. 2003;165:1059-70 pubmed..These data point out the interesting possibility that signal transduction pathways might directly influence the elongation step of RNA polymerase II transcription. ..
- Jiang H, Ma C, Lu Z, Kanost M. Beta-1,3-glucan recognition protein-2 (betaGRP-2)from Manduca sexta; an acute-phase protein that binds beta-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria and stimulate prophenoloxidase activation. Insect Biochem Mol Biol. 2004;34:89-100 pubmed..sexta, these results indicate that a complex system of protein sensors is an integral component of the insect immune system and that different recognition molecules have overlapping binding specificity and functions. ..
- Nakada D, Hirano Y, Sugimoto K. Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway. Mol Cell Biol. 2004;24:10016-25 pubmed..Our results provide a model in which the Mre11 complex and Exo1 cooperate in generating long ssDNA tracts and thereby facilitate Mec1 association with sites of DNA damage or replication block. ..
- Hajra S, Ghosh S, Jayaram M. The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation. J Cell Biol. 2006;174:779-90 pubmed..Poaching of a specific histone variant by the plasmid to mark its partitioning locus with a centromere tag reveals yet another one of the molecular trickeries it performs for achieving chromosome- like fidelity in segregation. ..
- Hill J, Brett C, Chyou A, Kallay L, Sakaguchi M, Rao R, et al. Vestibular hair bundles control pH with (Na+, K+)/H+ exchangers NHE6 and NHE9. J Neurosci. 2006;26:9944-55 pubmed..This mechanism allows the hair cell to remove H+ generated by Ca2+ pumping without ATP hydrolysis in the cell. ..
- Sahi C, Craig E. Network of general and specialty J protein chaperones of the yeast cytosol. Proc Natl Acad Sci U S A. 2007;104:7163-8 pubmed..However, a subset of Hsp70 functions requires specific J protein partners, likely demanding either sublocalization within the compartment or binding to specific client proteins. ..
- Li Z, Pogany J, Tupman S, Esposito A, Kinzy T, Nagy P. Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis. PLoS Pathog. 2010;6:e1001175 pubmed publisher..These roles for eEF1A are separate from its canonical role in host and viral protein translation, emphasizing critical functions for this abundant cellular protein during TBSV replication. ..
- Myers M, Ryazantsev S, Hicke L, Payne G. Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis. Dev Cell. 2016;37:162-73 pubmed publisher..These studies reveal a conserved role for calmodulin in regulating the intrinsic membrane-sculpting activity of endocytic N-BAR domains. ..
- Lawrimore J, Barry T, Barry R, York A, Friedman B, Cook D, et al. Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage. Mol Biol Cell. 2017;28:1701-1711 pubmed publisher..These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin-chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage. ..
- Burland T, Pallotta D, Tardif M, Lemieux G, Dove W. Fission yeast promoter-probe vectors based on hygromycin resistance. Gene. 1991;100:241-5 pubmed..We used the vectors to identify several genomic sequences from Physarum that provide promoter function in fission yeast. ..
- Robinson L, Hubbard E, Graves P, DePaoli Roach A, Roach P, Kung C, et al. Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A. 1992;89:28-32 pubmed..Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues. ..
- Sauer B, Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 1990;2:441-9 pubmed..The resulting integrants are predominantly simple single-copy insertions. Cre-mediated recombination thus provides a simple way to direct single-copy site-specific integration of exogenous DNA into the eukaryotic genome. ..
- Thon V, Khalil M, Cannon J. Isolation of human glycogen branching enzyme cDNAs by screening complementation in yeast. J Biol Chem. 1993;268:7509-13 pubmed..The human and yeast glycogen branching enzymes have a 67% identical amino acid sequence over a major portion of their length. The human gene is on chromosome 3. ..
- Baxter B, James P, Evans T, Craig E. SSI1 encodes a novel Hsp70 of the Saccharomyces cerevisiae endoplasmic reticulum. Mol Cell Biol. 1996;16:6444-56 pubmed..Interestingly, SSI1 deletion strains show a partial block in translocation of multiple proteins into the ER, suggesting that Ssi1p plays a direct role in the translocation process. ..
- Steinmetz E, Brow D. Control of pre-mRNA accumulation by the essential yeast protein Nrd1 requires high-affinity transcript binding and a domain implicated in RNA polymerase II association. Proc Natl Acad Sci U S A. 1998;95:6699-704 pubmed..In contrast, the RNA-binding activity of Nrd1 appears to be required both for down-regulation and for its essential function. ..
- Page M, Carr B, Anders K, Grimson A, Anderson P. SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast. Mol Cell Biol. 1999;19:5943-51 pubmed..We discuss these results with regard to the in vivo functions of SMG-2 and NMD...
- Lopinski J, Dinman J, Bruenn J. Kinetics of ribosomal pausing during programmed -1 translational frameshifting. Mol Cell Biol. 2000;20:1095-103 pubmed..Our in vitro and in vivo experiments can best be interpreted to mean that there are three methods by which ribosomes pass the frameshift site, only one of which results in frameshifting...
- Hung N, Johnson A. Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:3718-27 pubmed..Furthermore, these results suggest that Rei1 is needed for release of Arx1 from nascent 60S subunits after export to the cytoplasm but not for the subsequent nuclear import of Arx1. ..
- Hu R, Wang H, Xia Z, Varshavsky A. The N-end rule pathway is a sensor of heme. Proc Natl Acad Sci U S A. 2008;105:76-81 pubmed
- Loukin S, Zhou X, Kung C, Saimi Y. A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast. FASEB J. 2008;22:2405-15 pubmed publisher..It appears that stress on the cell wall induces Ca(2+) accumulation, adaptively anticipating the need in defense or repair against future stress, including osmotic stress. ..
- Ge Z, Wang H, Parthun M. Nuclear Hat1p complex (NuB4) components participate in DNA repair-linked chromatin reassembly. J Biol Chem. 2011;286:16790-9 pubmed publisher..Our data directly demonstrate the role of the nuclear HAT1 complex (NuB4) components in DNA repair-linked chromatin reassembly. ..
- Tsuchiya D, Gonzalez C, Lacefield S. The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:2848-61 pubmed publisher..We propose that Mad2 is an important meiotic cell cycle regulator that ensures the timely degradation of APC/C substrates and the proper orchestration of the meiotic divisions. ..
- Tebbets B, Yu Z, Stewart D, Zhao L, Jiang Y, Xu L, et al. Identification of antifungal natural products via Saccharomyces cerevisiae bioassay: insights into macrotetrolide drug spectrum, potency and mode of action. Med Mycol. 2013;51:280-9 pubmed publisher..Thus, this simple, high-throughput bioassay permitted us to screen microbial extracts, identify natural products as antifungal drugs, and expand our understanding of the activity of macrotetrolides. ..
- Rodriguez J, Tsukiyama T. ATR-like kinase Mec1 facilitates both chromatin accessibility at DNA replication forks and replication fork progression during replication stress. Genes Dev. 2013;27:74-86 pubmed publisher..These results suggest a possibility that Mec1 may facilitate DNA replication fork progression during replication stress by increasing chromatin accessibility around replication forks. ..
- Lo W, Lee M, Narayan M, Chou C, Park H. Polarization of diploid daughter cells directed by spatial cues and GTP hydrolysis of Cdc42 budding yeast. PLoS ONE. 2013;8:e56665 pubmed publisher..Moreover, modeling predicts different dynamics of Cdc42-GTP polarization when the landmark level and the initial level of Cdc42-GTP at the division site are perturbed by noise added in the model. ..
- Tien J, Fong K, Umbreit N, Payen C, Zelter A, Asbury C, et al. Coupling unbiased mutagenesis to high-throughput DNA sequencing uncovers functional domains in the Ndc80 kinetochore protein of Saccharomyces cerevisiae. Genetics. 2013;195:159-70 pubmed publisher..This unbiased mutagenesis approach can be generally applied to genes in S. cerevisiae to identify functional properties and domains...
- Deckard A, Anafi R, Hogenesch J, Haase S, Harer J. Design and analysis of large-scale biological rhythm studies: a comparison of algorithms for detecting periodic signals in biological data. Bioinformatics. 2013;29:3174-80 pubmed publisher..Additionally, these results can also be used to inform the design of large-scale biological rhythm experiments so that the resulting data can be used with these algorithms to detect periodic signals more effectively. ..
- Ladror D, Frey B, Scalf M, Levenstein M, Artymiuk J, Smith L. Methylation of yeast ribosomal protein S2 is elevated during stationary phase growth conditions. Biochem Biophys Res Commun. 2014;445:535-41 pubmed publisher..This suggests that yeast in the stationary phase increase methylation of RPS2 in order to regulate ribosomal synthesis. These results demonstrate the utility of mass spectrometry for quantifying dynamic changes in ribosomal PTMs. ..
- Alexander W, Peris D, Pfannenstiel B, Opulente D, Kuang M, Hittinger C. Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces. Fungal Genet Biol. 2016;89:10-17 pubmed publisher..This method provides an efficient means for producing novel synthetic hybrids for beverage and biofuel production, as well as for constructing tetraploids to be used for basic research in evolutionary genetics and genome stability. ..
- Wagner J, Alper H. Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances. Fungal Genet Biol. 2016;89:126-136 pubmed publisher..An emphasis is placed on synthetic parts and genome editing tools. Finally, we discuss examples of synthetic tools developed in other organisms that can be adapted or optimized for these hosts in the near future. ..
- Del Priore V, Snay C, Bahr A, Cole C. The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Mol Biol Cell. 1996;7:1601-21 pubmed
- Howell B, Lanier L, Frank R, Gertler F, Cooper J. The disabled 1 phosphotyrosine-binding domain binds to the internalization signals of transmembrane glycoproteins and to phospholipids. Mol Cell Biol. 1999;19:5179-88 pubmed..We propose that the PTB domain permits Dab1 to bind specifically to transmembrane proteins containing an NPXY internalization signal. ..
- Xin H, Lin W, Sumanasekera W, Zhang Y, Wu X, Wang Z. The human RAD18 gene product interacts with HHR6A and HHR6B. Nucleic Acids Res. 2000;28:2847-54 pubmed..Consistent with its role as a fundamental lesion bypass protein, the RAD18 gene is ubiquitously expressed in various human tissues. ..
- Sawai H, Okamoto Y, Luberto C, Mao C, Bielawska A, Domae N, et al. Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae. J Biol Chem. 2000;275:39793-8 pubmed..Labeling yeast cells with [(3)H]dihydrosphingosine showed that IPS were increased in the deletion mutant cells. This study identifies the first enzyme involved in catabolism of complex sphingolipids in S. cerevisiae. ..
- Vaena De Avalos S, Okamoto Y, Hannun Y. Activation and localization of inositol phosphosphingolipid phospholipase C, Isc1p, to the mitochondria during growth of Saccharomyces cerevisiae. J Biol Chem. 2004;279:11537-45 pubmed..A novel mechanism for activation of Isc1p through localization to mitochondria is proposed. The results also suggest a role for Isc1p-generated ceramides in optimal regulation of growth. ..
- Ursic D, Chinchilla K, Finkel J, Culbertson M. Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing. Nucleic Acids Res. 2004;32:2441-52 pubmed..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways. ..
- Pandit S, Lynn B, Rymond B. Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A. 2006;103:13700-5 pubmed..These and related genetic data support the existence of a Spp382p-dependent turnover pathway acting on defective spliceosomes. ..
- Stoj C, Augustine A, Zeigler L, Solomon E, Kosman D. Structural basis of the ferrous iron specificity of the yeast ferroxidase, Fet3p. Biochemistry. 2006;45:12741-9 pubmed..Indeed, this double mutant functions kinetically better as a laccase, albeit a relatively inefficient one. ..
- Carey M, Li B, Workman J. RSC exploits histone acetylation to abrogate the nucleosomal block to RNA polymerase II elongation. Mol Cell. 2006;24:481-7 pubmed..Thus, RSC can recognize acetylated nucleosomes and facilitate passage of Pol II through them. These data support the view that histone modifications regulate accessibility of the coding region to Pol II. ..
- Jonczyk M, Pathak K, Sharma M, Nagy P. Exploiting alternative subcellular location for replication: tombusvirus replication switches to the endoplasmic reticulum in the absence of peroxisomes. Virology. 2007;362:320-30 pubmed..This suggests that RNA viruses might have remarkable flexibility for using various host membranes for their replication. ..
- Van Wynsberghe P, Ahlquist P. 5' cis elements direct nodavirus RNA1 recruitment to mitochondrial sites of replication complex formation. J Virol. 2009;83:2976-88 pubmed publisher..These results establish RNA recruitment to the sites of replication complex formation as an essential, distinct, and selective early step in nodavirus replication...
- Akiyoshi B, Nelson C, Ranish J, Biggins S. Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit. Genes Dev. 2009;23:2887-99 pubmed publisher..These data suggest that Fin1 is a PP1 regulatory subunit whose spatial and temporal activity must be precisely controlled to ensure genomic stability. ..
- Guo J, Tian D, McKinney B, Hartman J. Recursive expectation-maximization clustering: a method for identifying buffering mechanisms composed of phenomic modules. Chaos. 2010;20:026103 pubmed publisher..It facilitates identification of phenomic modules, which contribute to buffering mechanisms that underlie cellular homeostasis and the regulation of phenotypic expression. ..
- Pike J, Henry R, Burgers P, Campbell J, Bambara R. An alternative pathway for Okazaki fragment processing: resolution of fold-back flaps by Pif1 helicase. J Biol Chem. 2010;285:41712-23 pubmed publisher..These results suggest an alternative pathway in which Pif1 removes Okazaki fragments initiated by fold-back flaps in vivo. ..
- Yeh Y, Shah K, Chou C, Hsiao H, Wrasman K, Stephan J, et al. The identification and analysis of phosphorylation sites on the Atg1 protein kinase. Autophagy. 2011;7:716-26 pubmed..In all, these studies identified a number of potential sites of regulation within Atg1 and will serve as a framework for future work with this enzyme. ..
- Alvarez Vasquez F, Riezman H, Hannun Y, Voit E. Mathematical modeling and validation of the ergosterol pathway in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e28344 pubmed publisher..Specifically, we simulated the action of drugs affecting sphingolipids in the endoplasmic reticulum and studied changes in ergosterol associated with microdomains of the plasma membrane (PM). ..
- Li S, Diakov T, Rizzo J, Kane P. Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress. Eukaryot Cell. 2012;11:282-91 pubmed publisher..Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification. ..
- Lester R, Withers B, Schultz M, Dickson R. Iron, glucose and intrinsic factors alter sphingolipid composition as yeast cells enter stationary phase. Biochim Biophys Acta. 2013;1831:726-36 pubmed publisher..The physiological rise in LCBs that we observe may serve to modulate nutrient transporters and possibly other membrane phenomena that contribute to enhanced stress resistance and survival in stationary phase. ..
- Kang P, Hood Degrenier J, Park H. Coupling of septins to the axial landmark by Bud4 in budding yeast. J Cell Sci. 2013;126:1218-26 pubmed publisher..Taken together, these results indicate that Bud4 functions as a platform that links septins to the axial landmark. ..
- Halladay J, Craig E. A heat shock transcription factor with reduced activity suppresses a yeast HSP70 mutant. Mol Cell Biol. 1995;15:4890-7 pubmed
- Halbrook J, Hoekstra M. Mutations in the Saccharomyces cerevisiae CDC1 gene affect double-strand-break-induced intrachromosomal recombination. Mol Cell Biol. 1994;14:8037-50 pubmed
- Dick F, Trumpower B. Heterologous complementation reveals that mutant alleles of QSR1 render 60S ribosomal subunits unstable and translationally inactive. Nucleic Acids Res. 1998;26:2442-8 pubmed..Velocity gradient analyses of the hybrid ribosomes after exposure to high concentrations of salt indicate that the decreased activity is due to lability of the hybrid 60S subunits. ..
- Reeder R. Regulation of RNA polymerase I transcription in yeast and vertebrates. Prog Nucleic Acid Res Mol Biol. 1999;62:293-327 pubmed..This suggests that multiple signaling pathways may also be involved. The involvement of ribosomal genes and their transcripts in events such as mitosis, cancer, and aging is discussed. ..
- Fairman C, Zhou X, Kung C. Potassium uptake through the TOK1 K+ channel in the budding yeast. J Membr Biol. 1999;168:149-57 pubmed..Thus, the yeast outwardly rectifying K+ channel can take up K+ into yeast cells, at least under certain conditions. ..
- Migeon J, Garfinkel M, Edgar B. Cloning and characterization of peter pan, a novel Drosophila gene required for larval growth. Mol Biol Cell. 1999;10:1733-44 pubmed..Overexpression of the wild-type gene causes cell death and disrupts the normal development of adult structures. The ppan gene family appears to have an essential and evolutionarily conserved role in cell growth. ..
- Stevens S, Abelson J. Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A. 1999;96:7226-31 pubmed
- Voisine C, Schilke B, Ohlson M, Beinert H, Marszalek J, Craig E. Role of the mitochondrial Hsp70s, Ssc1 and Ssq1, in the maturation of Yfh1. Mol Cell Biol. 2000;20:3677-84 pubmed
- Filippov V, Filippova M, Sehnal F, Gill S. Temporal and spatial expression of the cell-cycle regulator cul-1 in Drosophila and its stimulation by radiation-induced apoptosis. J Exp Biol. 2000;203:2747-56 pubmed..Zygotic transcription of the gene, however, resumes at early stages of embryogenesis. We also found an increase in cul-1 transcription in cultured cells treated with a lethal dose of gamma-irradiation. ..
- Sergienko E, Jordan F. Catalytic acid-base groups in yeast pyruvate decarboxylase. 3. A steady-state kinetic model consistent with the behavior of both wild-type and variant enzymes at all relevant pH values. Biochemistry. 2001;40:7382-403 pubmed
- Helfant A. Composition of the spindle pole body of Saccharomyces cerevisiae and the proteins involved in its duplication. Curr Genet. 2002;40:291-310 pubmed..In addition, genetic and biochemical studies have revealed the functional and physical relationships of certain spindle pole components and proteins important for SPB duplication. ..
- Viswanathan P, Ohn T, Chiang Y, Chen J, Denis C. Mouse CAF1 can function as a processive deadenylase/3'-5'-exonuclease in vitro but in yeast the deadenylase function of CAF1 is not required for mRNA poly(A) removal. J Biol Chem. 2004;279:23988-95 pubmed..These results indicate that at least in yeast the CAF1 RNase activity is not required for its in vivo function. ..
- Liu M, Tarsio M, Charsky C, Kane P. Structural and functional separation of the N- and C-terminal domains of the yeast V-ATPase subunit H. J Biol Chem. 2005;280:36978-85 pubmed..The N-terminal domain alone is sufficient for activation of ATP hydrolysis in V(1), but the C-terminal domain is essential for proper communication between the V(1) and V(o) sectors. ..
- Qin S, Parthun M. Recruitment of the type B histone acetyltransferase Hat1p to chromatin is linked to DNA double-strand breaks. Mol Cell Biol. 2006;26:3649-58 pubmed..These results indicate that Hat1p plays a direct role in DNA damage repair and is responsible for specific changes in histone modification that occur during the course of recombinational DNA repair. ..
- Ding M, Butler C, Saracco S, Fox T, Godard F, di Rago J, et al. Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins. Biochim Biophys Acta. 2008;1777:1147-56 pubmed publisher..This is the first time mutations have been created for three of the loci presented here, and nine of the resulting mutants have never been described before. ..
- Liu Y, Warfield L, Zhang C, Luo J, Allen J, Lang W, et al. Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol. 2009;29:4852-63 pubmed publisher..Genetic results suggest that Bur1 is essential for growth because it targets multiple factors that play distinct roles in transcription. ..
- Xiao J, Chen R, Pawlicki M, Tolbert T. Targeting a homogeneously glycosylated antibody Fc to bind cancer cells using a synthetic receptor ligand. J Am Chem Soc. 2009;131:13616-8 pubmed publisher..The methods described here can also be used to produce glycoprotein fragments for the chemoenzymatic synthesis of homogeneous glycoproteins. ..
- Dewe J, Whipple J, Chernyakov I, Jaramillo L, Phizicky E. The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications. RNA. 2012;18:1886-96 pubmed publisher..These results demonstrate that RTD interacts with the translation machinery and acts widely on hypomodified tRNAs. ..
- Kim J, Reed J. RELATCH: relative optimality in metabolic networks explains robust metabolic and regulatory responses to perturbations. Genome Biol. 2012;13:R78 pubmed publisher..This constraint-based approach will have broad utility where predictions of metabolic responses are needed. ..
- Paeschke K, Bochman M, Garcia P, Cejka P, Friedman K, Kowalczykowski S, et al. Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature. 2013;497:458-62 pubmed publisher..Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening. ..
- Adler H, Nallaseth F, Walter G, Tkachuk D. HRX leukemic fusion proteins form a heterocomplex with the leukemia-associated protein SET and protein phosphatase 2A. J Biol Chem. 1997;272:28407-14 pubmed..These results suggest that the leukemogenic effects of HRX fusion proteins may be related to interactions with SET and PP2A. ..
- Barlowe C. Coupled ER to Golgi transport reconstituted with purified cytosolic proteins. J Cell Biol. 1997;139:1097-108 pubmed..Ordering experiments using the dilution resistant intermediate and reversible Sec23p complex inhibition indicate Sec18p action is required before LMA1 function. ..
- Gardner K, Rine J, Fox C. A region of the Sir1 protein dedicated to recognition of a silencer and required for interaction with the Orc1 protein in saccharomyces cerevisiae. Genetics. 1999;151:31-44 pubmed..The approach used to find these mutations may be applicable to defining interaction surfaces on proteins involved in other processes that require the assembly of macromolecular complexes. ..
- Ungermann C, von Mollard G, Jensen O, Margolis N, Stevens T, Wickner W. Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion. J Cell Biol. 1999;145:1435-42 pubmed..Our data show that vacuole-vacuole fusion requires a cis-SNARE complex of five SNAREs, the t-SNAREs Vam3p and Vam7p and the v-SNAREs Nyv1p, Vti1p, and Ykt6p. ..
- Seals D, Eitzen G, Margolis N, Wickner W, Price A. A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A. 2000;97:9402-7 pubmed..HOPS initially associates with vacuole SNAREs in "cis" and, after release by priming, hops to Ypt7p, activating this Ypt/Rab switch to initiate docking. ..
- Xie Y, Varshavsky A. RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit. Proc Natl Acad Sci U S A. 2001;98:3056-61 pubmed
- Eitzen G, Thorngren N, Wickner W. Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking. EMBO J. 2001;20:5650-6 pubmed..Thus, multiple GTPases act in a defined sequence to regulate the docking steps of vacuole fusion. ..
- McPheeters D, Muhlenkamp P. Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol. 2003;23:4174-86 pubmed..Taken together, these data provide a detailed portrayal of the dynamic associations of proteins with the branch site-3' splice site region during spliceosome assembly and catalysis. ..
- Pereira S, Huang Y, Bobik E, Kinney A, Stecca K, Packer J, et al. A novel omega3-fatty acid desaturase involved in the biosynthesis of eicosapentaenoic acid. Biochem J. 2004;378:665-71 pubmed
- Smith J, Richardson N, Robinson A. Elevated expression temperature in a mesophilic host results in increased secretion of a hyperthermophilic enzyme and decreased cell stress. Biochim Biophys Acta. 2005;1752:18-25 pubmed publisher..We believe that this is the first evidence of in vivo effects of temperature on the secretion of hyperthermophilic proteins...
- Feske B, Kaluzna I, Stewart J. Enantiodivergent, biocatalytic routes to both taxol side chain antipodes. J Org Chem. 2005;70:9654-7 pubmed..This brief and atom-efficient route to both target enantiomers demonstrates both the synthetic utility of individual yeast reductases and the power of genomic strategies in making these catalysts available. ..
- Malik M, Nitiss K, Enriquez Rios V, Nitiss J. Roles of nonhomologous end-joining pathways in surviving topoisomerase II-mediated DNA damage. Mol Cancer Ther. 2006;5:1405-14 pubmed..Taken together, these results show a clear role for NHEJ in the repair of DNA damage induced by topoisomerase II-targeting agents and suggest that this pathway may participate in translocations generated by drugs, such as etoposide. ..
- Schacherer J, Ruderfer D, Gresham D, Dolinski K, Botstein D, Kruglyak L. Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains. PLoS ONE. 2007;2:e322 pubmed..These data and new visualization tools are accessible online in a new resource: the Yeast SNPs Browser (YSB; http://gbrowse.princeton.edu/cgi-bin/gbrowse/yeast_strains_snps) that is available to all researchers. ..
- Kupchak B, Garitaonandia I, Villa N, Mullen M, Weaver M, Regalla L, et al. Probing the mechanism of FET3 repression by Izh2p overexpression. Biochim Biophys Acta. 2007;1773:1124-32 pubmed..Finally, we show that the mechanism by which PAQR receptors activate signal transduction pathways is likely to be conserved from yeast to humans. ..
- Hood H, Neafsey D, Galagan J, Sachs M. Evolutionary roles of upstream open reading frames in mediating gene regulation in fungi. Annu Rev Microbiol. 2009;63:385-409 pubmed publisher..Some uORFs may have regulatory roles, while others may exist to insulate the genic reading frame from the negative impacts of upstream translation start sites in the mRNA 5' leader. ..
- Brechbuhl H, Gould N, Kachadourian R, Riekhof W, Voelker D, Day B. Glutathione transport is a unique function of the ATP-binding cassette protein ABCG2. J Biol Chem. 2010;285:16582-7 pubmed publisher..In addition, suppression of ABCG2 with short hairpin RNA or ABCG2 overexpression in human epithelial cells decreased or increased extracellular GSH levels, respectively. Our data indicate that ABCG2 is a novel GSH transporter...
- Piao H, Maclean Freed J, Mayinger P. Metabolic activation of the HOG MAP kinase pathway by Snf1/AMPK regulates lipid signaling at the Golgi. Traffic. 2012;13:1522-31 pubmed publisher..We conclude that a novel cross-talk between the HOG pathway and Snf1/AMPK is required for the metabolic control of lipid signaling at the Golgi. ..