Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Switzerland
Topic: saccharomyces cerevisiae
Publications188 found, 100 shown here
- Barabino S, Hubner W, Jenny A, Minvielle Sebastia L, Keller W. The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes Dev. 1997;11:1703-16 pubmed..Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit. ..
- Donze O, Picard D. Hsp90 binds and regulates Gcn2, the ligand-inducible kinase of the alpha subunit of eukaryotic translation initiation factor 2 [corrected]. Mol Cell Biol. 1999;19:8422-32 pubmed..Taken together, these data identify Gcn2 as a novel target for Hsp90, which plays a crucial role for the maturation and regulation of Gcn2. ..
- Liu J, Jeannin E, Picard D. The anti-estrogen hydroxytamoxifen is a potent antagonist in a novel yeast system. Biol Chem. 1999;380:1341-5 pubmed..While they all act as agonists for wild-type ERalpha, we have established a novel yeast assay system for anti-estrogens, in which at least the commonly used anti-estrogen hydroxytamoxifen is a potent antagonist. ..
- Galan J, Wiederkehr A, Seol J, Haguenauer Tsapis R, Deshaies R, Riezman H, et al. Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol Cell Biol. 2001;21:3105-17 pubmed..Importantly, Skp1p was the only major partner that copurified with Rcy1p. Our results thus suggest that a complex composed of Rcy1p and Skp1p but not other SCF components may play a direct role in recycling of internalized proteins. ..
- Martin D, Soulard A, Hall M. TOR regulates ribosomal protein gene expression via PKA and the Forkhead transcription factor FHL1. Cell. 2004;119:969-79 pubmed..Thus, we describe a signaling mechanism linking an environmental sensor to ribosome biogenesis. ..
- Gaigg B, Toulmay A, Schneiter R. Very long-chain fatty acid-containing lipids rather than sphingolipids per se are required for raft association and stable surface transport of newly synthesized plasma membrane ATPase in yeast. J Biol Chem. 2006;281:34135-45 pubmed..We thus conclude that the essential function of sphingolipids for membrane domain formation and stable surface delivery of Pma1p is provided by the C26 fatty acid that forms part of the yeast ceramide. ..
- Kilchert C, Weidner J, Prescianotto Baschong C, Spang A. Defects in the secretory pathway and high Ca2+ induce multiple P-bodies. Mol Biol Cell. 2010;21:2624-38 pubmed publisher..We propose that PB assembly in response to stress occurs at the ER and depends on intracellular signals that regulate PB number. ..
- Suzuki C, Suda K, Wang N, Schatz G. Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration. Science. 1994;264:273-6 pubmed..Lon- cells were also impaired in their ability to catalyze the energy-dependent degradation of several mitochondrial matrix proteins and they accumulated electron-dense inclusions in their mitochondrial matrix. ..
- Saner C, Weibel B, Wurgler F, Sengstag C. Metabolism of promutagens catalyzed by Drosophila melanogaster CYP6A2 enzyme in Saccharomyces cerevisiae. Environ Mol Mutagen. 1996;27:46-58 pubmed..Two other compounds, 7,12-dimethylbenz[a]anthracene (DMBA) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), were metabolized in the transformed strain to cytotoxic products. ..
- Louvion J, Abbas Terki T, Picard D. Hsp90 is required for pheromone signaling in yeast. Mol Biol Cell. 1998;9:3071-83 pubmed..These findings further corroborate the view that molecular chaperones must also be considered as transient or stable components of signal transduction pathways. ..
- Pierrat B, Ito M, Hinz W, Simonen M, Erdmann D, Chiesi M, et al. Uncoupling proteins 2 and 3 interact with members of the 14.3.3 family. Eur J Biochem. 2000;267:2680-7 pubmed..3.3 proteins potentiated the inhibitory effect of UCP3 overexpression on cell growth. These findings suggest that 14.3.3 proteins could be involved in the targeting of UCPs to the mitochondria. ..
- Flury I, Benachour A, Conzelmann A. YLL031c belongs to a novel family of membrane proteins involved in the transfer of ethanolaminephosphate onto the core structure of glycosylphosphatidylinositol anchors in yeast. J Biol Chem. 2000;275:24458-65 pubmed..e. the group that links the GPI lipid anchor to proteins, whereas Mcd4p and Gpi7p transfer ethanolaminephosphate onto the alpha1-4- and alpha1-6-linked mannoses of the GPI anchor, respectively. ..
- Pedrazzi G, Perrera C, Blaser H, Kuster P, Marra G, Davies S, et al. Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1. Nucleic Acids Res. 2001;29:4378-86 pubmed..We conclude that the hMLH1-BLM interaction is not essential for post-replicative MMR, but, more likely, is required for some aspect of genetic recombination. ..
- Friant S, Lombardi R, Schmelzle T, Hall M, Riezman H. Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J. 2001;20:6783-92 pubmed..The Pkc1p kinase that is phosphorylated by Pkh1/2p kinases and plays a role in endocytosis was identified as one of the downstream effectors of this signaling cascade. ..
- Poloni D, Simanis V. A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation. FEBS Lett. 2002;511:85-9 pubmed..Generation of a null allele demonstrates that the S. pombe sec13 gene is essential. ..
- Butty A, Perrinjaquet N, Petit A, Jaquenoud M, Segall J, Hofmann K, et al. A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO J. 2002;21:1565-76 pubmed..Thus, our results suggest that Bem1 functions in a positive feedback loop: local activation of Cdc24 produces Cdc42-GTP, which recruits Bem1. In turn, Bem1 stabilizes Cdc24 at the site of polarization, leading to apical growth. ..
- Flückiger S, Scapozza L, Mayer C, Blaser K, Folkers G, Crameri R. Immunological and structural analysis of IgE-mediated cross-reactivity between manganese superoxide dismutases. Int Arch Allergy Immunol. 2002;128:292-303 pubmed..Moreover, homology modelling provides a rational tool to identify conserved residues involved in defining cross-reactive B cell epitopes. ..
- Crespo J, Hall M. Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2002;66:579-91, table of contents pubmed..The current understanding of TOR and rapamycin is derived largely from studies with S. cerevisiae. In this review, we discuss the contributions made by S. cerevisiae to understanding rapamycin action and TOR function. ..
- Shimada K, Pasero P, Gasser S. ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase. Genes Dev. 2002;16:3236-52 pubmed..Our data suggest the existence of an S-phase-specific threshold that may be necessary to allow cells to tolerate damage-like DNA structures present at normal replication forks. ..
- Meier A, Thoma F. RNA polymerase I transcription factors in active yeast rRNA gene promoters enhance UV damage formation and inhibit repair. Mol Cell Biol. 2005;25:1586-95 pubmed..The data suggest that UAF is present in vivo in active and inactive promoters and that recruitment of CF and RNAP-I to active promoters generates a stable complex which inhibits repair. ..
- Ghugtyal V, Vionnet C, Roubaty C, Conzelmann A. CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae. Mol Microbiol. 2007;65:1493-502 pubmed..Ceramide remodelling is not required for the normal integration of GPI proteins into the cell wall. All remodelling reactions are dependent on prior removal of the inositol-linked fatty acid by Bst1p. ..
- Arneric M, Lingner J. Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast. EMBO Rep. 2007;8:1080-5 pubmed
- Kurz T, Chou Y, Willems A, Meyer Schaller N, Hecht M, Tyers M, et al. Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation. Mol Cell. 2008;29:23-35 pubmed publisher..We show that Dcn1 is necessary and sufficient for cullin neddylation in a purified recombinant system. Taken together, these data demonstrate that Dcn1 is a scaffold-like E3 ligase for cullin neddylation. ..
- Aymoz D, Wosika V, Durandau E, Pelet S. Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters. Nat Commun. 2016;7:11304 pubmed publisher..They display distinct expression dynamics giving rise to strikingly different instantaneous expression noise. ..
- Schneider A, Behrens M, Scherer P, Pratje E, Michaelis G, Schatz G. Inner membrane protease I, an enzyme mediating intramitochondrial protein sorting in yeast. EMBO J. 1991;10:247-54 pubmed..This 'inner membrane protease I' shares several key features with the leader peptidase of Escherichia coli and the signal peptidase of the endoplasmic reticulum. ..
- Buvelot Frei S, Rahl P, Nussbaum M, Briggs B, Calero M, Janeczko S, et al. Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p. Mol Cell Biol. 2006;26:7299-317 pubmed
- Breitling J, Aebi M. N-linked protein glycosylation in the endoplasmic reticulum. Cold Spring Harb Perspect Biol. 2013;5:a013359 pubmed publisher..This article focuses on the processes in the ER. Based on the highly conserved nature of this pathway we concentrate on the mechanisms in the eukaryotic model organism Saccharomyces cerevisiae. ..
- Schmidt A, Bickle M, Beck T, Hall M. The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Cell. 1997;88:531-42 pubmed..Thus, TOR2 signals to the actin cytoskeleton through a GTPase switch composed of RHO1, RHO2, ROM2, and SAC7. TOR2 activates this switch via ROM2, possibly via the ROM2 PH domain. ..
- Stagljar I, Korostensky C, Johnsson N, te Heesen S. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci U S A. 1998;95:5187-92 pubmed..Specific interactions are detected between Wbp1p and Ost1p, but not between Wbp1p and Alg5p. The new system might be useful as a genetic and biochemical tool for the analysis of interactions between membrane proteins in vivo. ..
- Koehler C, Murphy M, Bally N, Leuenberger D, Oppliger W, Dolfini L, et al. Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane. Mol Cell Biol. 2000;20:1187-93 pubmed..We suggest that Tim18p functions in the assembly and stabilization of the TIM22 complex but does not directly participate in protein insertion into the inner membrane. ..
- Weber W, Daoud El Baba M, Fussenegger M. Synthetic ecosystems based on airborne inter- and intrakingdom communication. Proc Natl Acad Sci U S A. 2007;104:10435-40 pubmed
- Frank C, Aebi M. ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis. Glycobiology. 2005;15:1156-63 pubmed..This report completes the list of lumen-oriented glycosyltransferases required for the assembly of the LLO. ..
- Magliano P, Flipphi M, Arpat B, Delessert S, Poirier Y. Contributions of the peroxisome and ?-oxidation cycle to biotin synthesis in fungi. J Biol Chem. 2011;286:42133-40 pubmed publisher..These results suggest that alternative pathways for synthesis of the pimelate intermediate exist in bacteria and eukaryotes and that Saccharomyces species use a pathway different from that used by the majority of fungi. ..
- Preker P, Lingner J, Minvielle Sebastia L, Keller W. The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase. Cell. 1995;81:379-89 pubmed..We propose a model in which PF I tethers PAP1 to CF I, thereby conferring specificity to poly(A) polymerase for pre-mRNA substrates. ..
- Blondel M, Alepuz P, Huang L, Shaham S, Ammerer G, Peter M. Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p. Genes Dev. 1999;13:2284-300 pubmed..Taken together, our results suggest that nuclear export of Far1p by Msn5p/Ste21p coordinates the two separable functions of Far1p during mating. ..
- Köffel R, Schneiter R. Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:1018-25 pubmed..These data thus provide the first link between fat degradation and the transcriptional control of lipase activity in yeast. ..
- Burda P, Jakob C, Beinhauer J, Hegemann J, Aebi M. Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyltransferases. Glycobiology. 1999;9:617-25 pubmed..This substrate specificity of the different transferases explains the ordered assembly of the complex structure of lipid-linked Glc3Man9GlcNAc2 in the endoplasmic reticulum. ..
- Crespo J, Daicho K, Ushimaru T, Hall M. The GATA transcription factors GLN3 and GAT1 link TOR to salt stress in Saccharomyces cerevisiae. J Biol Chem. 2001;276:34441-4 pubmed..In summary, our results suggest that TOR plays a role in the general response to saline stress by regulating the transcription of ENA1 via GLN3 and GAT1. ..
- Prescianotto Baschong C, Riezman H. Ordering of compartments in the yeast endocytic pathway. Traffic. 2002;3:37-49 pubmed..On the other hand, traffic to the early recycling endosome is less dependent on Rab5 homologs and does not require Vps27p. ..
- Uttenweiler A, Schwarz H, Neumann H, Mayer A. The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell. 2007;18:166-75 pubmed..Our data suggest that the VTC complex is an important constituent of autophagic tubes and that it is required for scission of microautophagic vesicles from these tubes. ..
- Yang M, Geli V, Oppliger W, Suda K, James P, Schatz G. The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein. J Biol Chem. 1991;266:6416-23 pubmed..A purified artificial precursor protein bound to the holoenzyme can be photocross-linked to the MAS2 subunit. ..
- Interthal H, Bellocq C, Bahler J, Bashkirov V, Edelstein S, Heyer W. A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO J. 1995;14:1057-66 pubmed..Taken together these three lines of evidence constitute compelling evidence for a role of Sep1 as an accessory protein in microtubule function in the yeast S.cerevisiae. ..
- Munn A, Stevenson B, Geli M, Riezman H. end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae. Mol Biol Cell. 1995;6:1721-42 pubmed..We propose that Vrp1p, Rvs161p, and Rvs167p are components of a cytoskeletal structure that contains actin and fimbrin and that is required for formation of endocytic vesicles at the plasma membrane. ..
- Campbell K, Cooper S, Dessing M, Yates S, Buder A. Interaction of p59fyn kinase with the dynein light chain, Tctex-1, and colocalization during cytokinesis. J Immunol. 1998;161:1728-37 pubmed
- Fahrenkrog B, Hurt E, Aebi U, Pante N. Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol. 1998;143:577-88 pubmed..Accordingly, Nsp1p resides in three distinct subcomplexes which are located at the entry and exit of the central gated channel and at the terminal ring of the nuclear basket. ..
- Pfander B, Matos J. Control of Mus81 nuclease during the cell cycle. FEBS Lett. 2017;591:2048-2056 pubmed publisher..Mus81 function is known to be enhanced upon entry into M phase in budding yeast and human cells. Based on recent findings, we discuss here an updated model of Mus81 control during the cell cycle. ..
- Baars T, Petri S, Peters C, Mayer A. Role of the V-ATPase in regulation of the vacuolar fission-fusion equilibrium. Mol Biol Cell. 2007;18:3873-82 pubmed..Its dual involvement in vacuole fission and fusion suggests the V-ATPase as a potential regulator of vacuolar morphology and membrane dynamics. ..
- Kuhne C, Linder P. A new pair of B-type cyclins from Saccharomyces cerevisiae that function early in the cell cycle. EMBO J. 1993;12:3437-47 pubmed..The clb5 mutant phenotype is somewhat more pronounced in a double null mutant. Both cyclins have the potential to interact with the p34CDC28 kinase in vivo. ..
- Berset C, Zurbriggen A, Djafarzadeh S, Altmann M, Trachsel H. RNA-binding activity of translation initiation factor eIF4G1 from Saccharomyces cerevisiae. RNA. 2003;9:871-80 pubmed..These data suggest that RNA-binding activity is required for eIF4G1 function. ..
- Uttenweiler A, Schwarz H, Mayer A. Microautophagic vacuole invagination requires calmodulin in a Ca2+-independent function. J Biol Chem. 2005;280:33289-97 pubmed..Kinetic analysis indicates that calmodulin is required for autophagic tube formation rather than for the final scission of vesicles from the tip of the tube. ..
- Pagac M, Vazquez H, Bochud A, Roubaty C, Knöpfli C, Vionnet C, et al. Topology of the microsomal glycerol-3-phosphate acyltransferase Gpt2p/Gat1p of Saccharomyces cerevisiae. Mol Microbiol. 2012;86:1156-66 pubmed publisher..Thus, the first step in biosynthesis of phosphatidic acid in yeast seems to occur in the ER lumen and substrates may have to cross the ER membrane. ..
- Desrivieres S, Cooke F, Parker P, Hall M. MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae. J Biol Chem. 1998;273:15787-93 pubmed..These results suggest that MSS4 is the major PI(4)P 5-kinase in yeast and provide a link between phosphoinositide metabolism and organization of the actin cytoskeleton in vivo. ..
- Muniz M, Nuoffer C, Hauri H, Riezman H. The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles. J Cell Biol. 2000;148:925-30 pubmed..Gap1p, which was not affected by emp24 mutation, was not cross-linked. These results suggest that the Emp24 complex acts as a cargo receptor in vesicle biogenesis from the ER. ..
- Canonaco F, Schlattner U, Pruett P, Wallimann T, Sauer U. Functional expression of phosphagen kinase systems confers resistance to transient stresses in Saccharomyces cerevisiae by buffering the ATP pool. J Biol Chem. 2002;277:31303-9 pubmed..Thus, our results demonstrate that temporal energy buffering is an intrinsic property of phosphagen kinases that can be transferred to phylogenetically very distant organisms. ..
- Preker P, Keller W. The HAT helix, a repetitive motif implicated in RNA processing. Trends Biochem Sci. 1998;23:15-6 pubmed
- Dubrana K, van Attikum H, Hediger F, Gasser S. The processing of double-strand breaks and binding of single-strand-binding proteins RPA and Rad51 modulate the formation of ATR-kinase foci in yeast. J Cell Sci. 2007;120:4209-20 pubmed..These observations led to a model that links end-processing and competition between different ssDNA-binding factors with Mec1-Ddc2 focus formation and checkpoint activation. ..
- Crespo J, Powers T, Fowler B, Hall M. The TOR-controlled transcription activators GLN3, RTG1, and RTG3 are regulated in response to intracellular levels of glutamine. Proc Natl Acad Sci U S A. 2002;99:6784-9 pubmed..Furthermore, as glutamine starvation affects only a subset of TOR-controlled transcription factors, TOR appears to discriminate between different nutrient conditions to elicit a response appropriate to a given condition. ..
- Keller Seitz M, Certa U, Sengstag C, Würgler F, Sun M, Fasullo M. Transcriptional response of yeast to aflatoxin B1: recombinational repair involving RAD51 and RAD1. Mol Biol Cell. 2004;15:4321-36 pubmed..Thus the transcriptional profile after aflatoxin B(1) exposure may elucidate the genotoxic properties of aflatoxin B(1). ..
- Zhu Y, Fraering P, Vionnet C, Conzelmann A. Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae. Biochim Biophys Acta. 2005;1735:79-88 pubmed..Shutting down ER-associated and vacuolar protein degradation pathways has no effect on the levels of Gpi17p or other transamidase subunits. ..
- Pagac M, de la Mora H, Duperrex C, Roubaty C, Vionnet C, Conzelmann A. Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae. J Biol Chem. 2011;286:36438-47 pubmed publisher..The implications of these findings are discussed. ..
- Leighton J, Schatz G. An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast. EMBO J. 1995;14:188-95 pubmed..Atm1p is the first mitochondrial member of the ABC family to be identified and the only eukaryotic ABC transporter that has been shown to be necessary for normal cellular growth...
- Gattiker A, Rischatsch R, Demougin P, Voegeli S, Dietrich F, Philippsen P, et al. Ashbya Genome Database 3.0: a cross-species genome and transcriptome browser for yeast biologists. BMC Genomics. 2007;8:9 pubmed..The Ashbya Genome Database (AGD) 3.0 is an innovative cross-species genome and transcriptome browser based on release 40 of the Ensembl developer environment...
- Leidel S, Pedrioli P, Bucher T, Brost R, Costanzo M, Schmidt A, et al. Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature. 2009;458:228-32 pubmed publisher..Finally, functional analysis reveals that the thiolation function of Urm1p is critical to regulate cellular responses to nutrient starvation and oxidative stress conditions, most likely by increasing translation fidelity. ..
- Paccaud J, Reith W, Carpentier J, Ravazzola M, Amherdt M, Schekman R, et al. Cloning and functional characterization of mammalian homologues of the COPII component Sec23. Mol Biol Cell. 1996;7:1535-46 pubmed..The exact function of hSec23Bp remains to be determined. ..
- Nasr F, Filipowicz W. Characterization of the Saccharomyces cerevisiae cyclic nucleotide phosphodiesterase involved in the metabolism of ADP-ribose 1",2"-cyclic phosphate. Nucleic Acids Res. 2000;28:1676-83 pubmed..Likewise, overexpression of Cpd1p had no effect on yeast growth. These results do not implicate an important role for Appr>p or Appr-1"p in yeast cells grown under standard laboratory conditions. ..
- Galan J, Peter M. Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism. Proc Natl Acad Sci U S A. 1999;96:9124-9 pubmed
- Laroche T, Martin S, Tsai Pflugfelder M, Gasser S. The dynamics of yeast telomeres and silencing proteins through the cell cycle. J Struct Biol. 2000;129:159-74 pubmed..Using live GFP fluorescence we show that the diffuse mitotic distribution of GFP-Rap1p is restored to the interphase pattern of foci in early G1-phase. ..
- Kramer A, Legrain P, Mulhauser F, Gröning K, Brosi R, Bilbe G. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. Nucleic Acids Res. 1994;22:5223-8 pubmed..The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution. ..
- Bahler J, Hagens G, Holzinger G, Scherthan H, Heyer W. Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase. Chromosoma. 1994;103:129-41 pubmed..The pachytene arrest in sep1 cells is likely to be regulatory, but is clearly different from the RAD9 checkpoint in meiotic prophase, which occurs prior to the pachytene stage. ..
- Thaminy S, Auerbach D, Arnoldo A, Stagljar I. Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res. 2003;13:1744-53 pubmed..We expect the split-ubiquitin membrane YTH technology to be valuable for the identification of potential interacting partners of integral membrane proteins from many model organisms. ..
- Bosson R, Jaquenoud M, Conzelmann A. GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor. Mol Biol Cell. 2006;17:2636-45 pubmed..GUP1 is highly conserved among fungi and protozoa and the gup1Delta phenotype is partially corrected by GUP1 homologues of Aspergillus fumigatus and Trypanosoma cruzi. ..
- Hall M, Craik C, Hiraoka Y. Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal. Proc Natl Acad Sci U S A. 1990;87:6954-8 pubmed..We propose that the two signals in alpha 2 are functionally distinct and act at different steps in a localization pathway. ..
- Schmidt A, Beck T, Koller A, Kunz J, Hall M. The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease. EMBO J. 1998;17:6924-31 pubmed..Thus, the TOR nutrient signalling pathway also controls growth by inhibiting a stationary phase (G0) programme. The control of NPR1 by TOR is analogous to the control of p70 s6 kinase and 4E-BP1 by mTOR in mammalian cells. ..
- McLoughlin P, Ehler E, Carlile G, Licht J, Schafer B. The LIM-only protein DRAL/FHL2 interacts with and is a corepressor for the promyelocytic leukemia zinc finger protein. J Biol Chem. 2002;277:37045-53 pubmed..This is the first reported incidence of a bona fide FHL protein-mediated corepression and supports the notion of these proteins having a role as coregulators of tissue-specific gene expression. ..
- Duncker B, Shimada K, Tsai Pflugfelder M, Pasero P, Gasser S. An N-terminal domain of Dbf4p mediates interaction with both origin recognition complex (ORC) and Rad53p and can deregulate late origin firing. Proc Natl Acad Sci U S A. 2002;99:16087-92 pubmed..Moreover, its overexpression perturbs the regulation of late, but not early, origin firing in wild-type cells after treatment with hydroxyurea. ..
- Haeuptle M, Pujol F, Neupert C, Winchester B, Kastaniotis A, Aebi M, et al. Human RFT1 deficiency leads to a disorder of N-linked glycosylation. Am J Hum Genet. 2008;82:600-6 pubmed publisher..RFT1 deficiency in both yeast and human cells leads to the accumulation of incomplete DolPP-GlcNAc(2)Man(5) and to a profound glycosylation disorder in humans. ..
- Lingner J, Kellermann J, Keller W. Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae. Nature. 1991;354:496-8 pubmed..Poly(A) polymerase purified from recombinant Escherichia coli has the same physical and biochemical properties as the yeast enzyme. The yeast poly(A) polymerase shares features of sequence with its mammalian homologue. ..
- Rospert S, Junne T, Glick B, Schatz G. Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES. FEBS Lett. 1993;335:358-60 pubmed..This gene encodes a protein of 11,372 Da that is imported into the mitochondrial matrix without detectable cleavage. Haploid cells lacking a functional copy of CPN10 fail to grow at temperatures between 23 and 37 degrees C. ..
- Bezzubova O, Schmidt H, Ostermann K, Heyer W, Buerstedde J. Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes. Nucleic Acids Res. 1993;21:5945-9 pubmed..High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination. ..
- Ziak M, Meier M, Etter K, Roth J. Two isoforms of trimming glucosidase II exist in mammalian tissues and cell lines but not in yeast and insect cells. Biochem Biophys Res Commun. 2001;280:363-7 pubmed..The ubiquitous occurrence of the two glucosidase II isoforms in mammalian tissues and cell lines might be indicative of a special function of each isoform. ..
- Schlecht U, Erb I, Demougin P, Robine N, Borde V, van Nimwegen E, et al. Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast. Mol Biol Cell. 2008;19:2193-207 pubmed publisher..The present study revealed numerous loci previously unknown to be under Abf1 control, and it yielded evidence for the protein's variable DNA binding pattern during mitotic growth and meiotic development. ..
- De Virgilio C, Bürckert N, Barth G, Neuhaus J, Boller T, Wiemken A. Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast. 1992;8:1043-51 pubmed..cerevisiae. Surprisingly, disruption of the ACS1 gene did not affect growth on media containing ethanol as the sole carbon source, demonstrating that there are alternative pathways leading to acetyl-CoA under these conditions. ..
- Koehler C, Merchant S, Oppliger W, Schmid K, Jarosch E, Dolfini L, et al. Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins. EMBO J. 1998;17:6477-86 pubmed..Tim9p is a new subunit of the TIM machinery that guides hydrophobic inner membrane proteins across the aqueous intermembrane space. ..
- Mueller P, Grueter P, Hinnebusch A, Trachsel H. A ribosomal protein is required for translational regulation of GCN4 mRNA. Evidence for involvement of the ribosome in eIF2 recycling. J Biol Chem. 1998;273:32870-7 pubmed
- Drogen F, O Rourke S, Stucke V, Jaquenoud M, Neiman A, Peter M. Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo. Curr Biol. 2000;10:630-9 pubmed..This mechanism may serve as a paradigm for the activation of mammalian MEKKs. ..
- Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed..Thus, the distinct TOR complexes account for the diversity, specificity, and selective rapamycin inhibition of TOR signaling. TORC1 and possibly TORC2 are conserved from yeast to man. ..
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