Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Sweden
Topic: saccharomyces cerevisiae
Publications192 found, 100 shown here
- Palm C, Netzereab S, Hällbrink M. Quantitatively determined uptake of cell-penetrating peptides in non-mammalian cells with an evaluation of degradation and antimicrobial effects. Peptides. 2006;27:1710-6 pubmed..In addition, these CPPs showed antimicrobial activity. ..
- Boban M, Ljungdahl P. Dal81 enhances Stp1- and Stp2-dependent transcription necessitating negative modulation by inner nuclear membrane protein Asi1 in Saccharomyces cerevisiae. Genetics. 2007;176:2087-97 pubmed..Conversely, the high levels of processed Stp1 and Stp2 that accumulate in the nucleus of induced cells activate transcription in the absence of Dal81. ..
- Then Bergh F, Flinn E, Svaren J, Wright A, Hörz W. Comparison of nucleosome remodeling by the yeast transcription factor Pho4 and the glucocorticoid receptor. J Biol Chem. 2000;275:9035-42 pubmed..Our results show that the DNA-binding domains of the two factors play critical roles in determining how chromatin structure is modified during promoter activation. ..
- Karlberg T, Lecerof D, Gora M, Silvegren G, Labbe Bois R, Hansson M, et al. Metal binding to Saccharomyces cerevisiae ferrochelatase. Biochemistry. 2002;41:13499-506 pubmed..The latter site is also located at the surface of the molecule and thought to be involved in initial metal binding and regulation. ..
- Davidsson R, Johansson B, Passoth V, Bengtsson M, Laurell T, Emneus J. Microfluidic biosensing systems. Part II. Monitoring the dynamic production of glucose and ethanol from microchip-immobilised yeast cells using enzymatic chemiluminescent micro-biosensors. Lab Chip. 2004;4:488-94 pubmed
- Alao J, Olesch J, Sunnerhagen P. Inhibition of type I histone deacetylase increases resistance of checkpoint-deficient cells to genotoxic agents through mitotic delay. Mol Cancer Ther. 2009;8:2606-15 pubmed publisher..Effective use of these agents as chemosensitizers and radiosensitizers may require specific treatment schedules that circumvent their inhibition of cell cycle progression. ..
- Andersson V, Hanzén S, Liu B, Molin M, Nystrom T. Enhancing protein disaggregation restores proteasome activity in aged cells. Aging (Albany NY). 2013;5:802-12 pubmed..The data supports the existence of a negative feedback loop that accelerates aging by exacerbating proteostatic decline once misfolded and aggregation-prone proteins reach a critical level. ..
- Gustavsson A, van Niekerk D, Adiels C, Kooi B, Goksör M, Snoep J. Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells. FEBS J. 2014;281:2784-93 pubmed publisher..mib.ac.uk/webMathematica/UItester.jsp?modelName=gustavsson5. [Database section added 14 May 2014 after original online publication]. ..
- Johansson M, Chen X, Milanova S, Santos C, Petranovic D. PUFA-induced cell death is mediated by Yca1p-dependent and -independent pathways, and is reduced by vitamin C in yeast. FEMS Yeast Res. 2016;16:fow007 pubmed publisher..When deleting YCA1, the caspase-like activity and the oxidative stress decreased and although the lifespan was slightly prolonged, the phenotype could not be fully reversed, pointing that Yca1p was not the main executor of cell death. ..
- Jokipii Lukkari S, Kastaniotis A, Parkash V, Sundström R, Leiva Eriksson N, Nymalm Y, et al. Dual targeted poplar ferredoxin NADP(+) oxidoreductase interacts with hemoglobin 1. Plant Sci. 2016;247:138-49 pubmed publisher..The structural modelling results indicate that PttHb1 and PtthFNR are able to interact as NO dioxygenase. This is the first report on dual targeting of central plant enzyme FNR to plastids and cytosol. ..
- Xue Y, Lipscomb W. Location of the active site of allosteric chorismate mutase from Saccharomyces cerevisiae, and comments on the catalytic and regulatory mechanisms. Proc Natl Acad Sci U S A. 1995;92:10595-8 pubmed..This Glu-246, modeled close to the ether oxygen of chorismate in YCM, may function as a polarizing or ionizable group, which provides another facet to the catalytic mechanism. ..
- Hohmann S, Meacock P. Thiamin metabolism and thiamin diphosphate-dependent enzymes in the yeast Saccharomyces cerevisiae: genetic regulation. Biochim Biophys Acta. 1998;1385:201-19 pubmed..Apparently, the production of ThDP and of the enzymes using this cofactor is coordinately regulated. Future research will focus on the elucidation of the molecular mechanisms of this novel type of regulation. ..
- Petersson A, Almeida J, Modig T, Karhumaa K, Hahn Hägerdal B, Gorwa Grauslund M, et al. A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance. Yeast. 2006;23:455-64 pubmed..Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity...
- Molin M, Blomberg A. Dihydroxyacetone detoxification in Saccharomyces cerevisiae involves formaldehyde dissimilation. Mol Microbiol. 2006;60:925-38 pubmed..We conclude that mechanisms for DHA growth and detoxification appear complex and that the evolutionary strive to minimize detrimental effects of this intracellular metabolite links to both formaldehyde and glutathione metabolism. ..
- Persson C, Subramaniyam D, Stevens T, Janciauskiene S. Do native and polymeric alpha1-antitrypsin activate human neutrophils in vitro?. Chest. 2006;129:1683-92 pubmed
- Alriksson B, Sjöde A, Nilvebrant N, Jönsson L. Optimal conditions for alkaline detoxification of dilute-acid lignocellulose hydrolysates. Appl Biochem Biotechnol. 2006;129-132:599-611 pubmed..The considerable difference in the amount of precipitate generated after treatment with different types of alkali appears critical for industrial implementation. ..
- Di Y, Tamás M. Regulation of the arsenic-responsive transcription factor Yap8p involves the ubiquitin-proteasome pathway. J Cell Sci. 2007;120:256-64 pubmed..In this way, regulated degradation contributes to Yap8p control. ..
- Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J. Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng. 2013;15:48-54 pubmed publisher..This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products. ..
- Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed..These findings suggest that Mso1p is a component of the secretory vesicle docking complex whose function is closely associated with that of Sec1p. ..
- Nourizad N, Ehn M, Gharizadeh B, Hober S, Nyrén P. Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum). Protein Expr Purif. 2003;27:229-37 pubmed..By enzymatic removal of N-glycans, a single band corresponding to a molecular mass of 48kDa was detected. The recombinant apyrase was found to function well when it was used in combination with the Pyrosequencing technology. ..
- Ekengren S, Hultmark D. Drosophila cecropin as an antifungal agent. Insect Biochem Mol Biol. 1999;29:965-72 pubmed..Even dense cultures of Saccharomyces cerevisiae can be cleared by micromolar concentrations of cecropin, whereas Geotrichum candidum is unaffected by cecropin when tested in a dense culture. ..
- Pratt J, Mouillon J, Lagerstedt J, Pattison Granberg J, Lundh K, Persson B. Effects of methylphosphonate, a phosphate analogue, on the expression and degradation of the high-affinity phosphate transporter Pho84, in Saccharomyces cerevisiae. Biochemistry. 2004;43:14444-53 pubmed..Altogether, these observations suggest that the Pho84 transporter is regulated not only at the transcriptional level but also by a direct molecule-sensing mechanism at the protein level. ..
- Aronsson K, Rönner U, Borch E. Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae in relation to membrane permeabilization and subsequent leakage of intracellular compounds due to pulsed electric field processing. Int J Food Microbiol. 2005;99:19-32 pubmed..Results suggest that E. coli and L. innocua cells, which took up PI, lost their ability to multiply, whereas cells of S. cerevisiae, which also took up PI, were not necessarily lethally permeabilized. ..
- Ding B, Liénard M, Wang H, Zhao C, Löfstedt C. Terminal fatty-acyl-CoA desaturase involved in sex pheromone biosynthesis in the winter moth (Operophtera brumata). Insect Biochem Mol Biol. 2011;41:715-22 pubmed publisher..These results provide evidence that a terminal desaturation step is involved in the winter moth pheromone biosynthesis, prior to the decarboxylation. ..
- de Jong B, Siewers V, Nielsen J. Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels. Curr Opin Biotechnol. 2012;23:624-30 pubmed publisher..This review will cover the recent technological developments that support improvement of the advanced biofuels 1-butanol, biodiesels and jetfuels. ..
- Hu G, Ronne H. Overexpression of yeast PAM1 gene permits survival without protein phosphatase 2A and induces a filamentous phenotype. J Biol Chem. 1994;269:3429-35 pubmed..The PAM1 gene encodes a hydrophilic 93-kDa protein that contains two coiled coil motifs and has a highly basic C-terminal tail. High level overexpression of PAM1 inhibits growth and induces a filamentous phenotype. ..
- Gustavsson M, Ronne H. Evidence that tRNA modifying enzymes are important in vivo targets for 5-fluorouracil in yeast. RNA. 2008;14:666-74 pubmed publisher..This suggests that tRNA destabilization contributes to 5-FU cytotoxicity in wild-type cells and provides a possible explanation why hyperthermia can enhance the effect of 5-FU in cancer therapy. ..
- Khoomrung S, Chumnanpuen P, Jansa ard S, Nookaew I, Nielsen J. Fast and accurate preparation fatty acid methyl esters by microwave-assisted derivatization in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2012;94:1637-46 pubmed publisher..The new microwave-assisted derivatization method facilitates the preparation of FAMEs directly from yeast cells, but the method is likely to also be applicable for other biological samples. ..
- Samyn D, Ruiz Pavón L, Andersson M, Popova Y, Thevelein J, Persson B. Mutational analysis of putative phosphate- and proton-binding sites in the Saccharomyces cerevisiae Pho84 phosphate:H(+) transceptor and its effect on signalling to the PKA and PHO pathways. Biochem J. 2012;445:413-22 pubmed publisher
- Helgstrand C, Hasan M, Uysal H, Haeggstrom J, Thunnissen M. A leukotriene A4 hydrolase-related aminopeptidase from yeast undergoes induced fit upon inhibitor binding. J Mol Biol. 2011;406:120-34 pubmed publisher..Inasmuch as bestatin is a structural analogue of a leucyl dipeptide and may be regarded as a transition state mimic, our results imply that the enzyme undergoes induced fit during substrate binding and turnover. ..
- Görgens J, Van Zyl W, Knoetze J, Hahn Hagerdal B. The metabolic burden of the PGK1 and ADH2 promoter systems for heterologous xylanase production by Saccharomyces cerevisiae in defined medium. Biotechnol Bioeng. 2001;73:238-45 pubmed..This was most likely due to an increased energetic demand for the expression of a foreign gene and/or a competition for limiting amounts of transcription or translation factors, biosynthetic precursors or metabolic energy. ..
- Nookaew I, Papini M, Pornputtapong N, Scalcinati G, Fagerberg L, Uhlén M, et al. A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case study in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:10084-97 pubmed publisher
- Hong K, Nielsen J. Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose. Metab Eng. 2013;16:78-86 pubmed publisher..These results indicate that antagonistic pleiotropy is the dominant mechanism in the observed trade-off, and it is likely realized by changes in glucose signaling. ..
- Mountain H, Byström A, Larsen J, Korch C. Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae. Yeast. 1991;7:781-803 pubmed..In addition to the strong repression by methionine, MET5 is also regulated by the general control. ..
- Ljungdahl P, Daignan Fornier B. Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae. Genetics. 2012;190:885-929 pubmed publisher..Thanks to the remarkable sophistication offered by the yeast experimental system, a picture of the intimate connections between the metabolomic and the transcriptome is becoming clear. ..
- Geijer C, Medrala Klein D, Petelenz Kurdziel E, Ericsson A, Smedh M, Andersson M, et al. Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery. FEBS J. 2013;280:3854-67 pubmed publisher..Furthermore, we found that the period of Hog1 nuclear residence affects the amplitude of the transcriptional response rather than the spectrum of responsive genes. ..
- Sárvári Horváth I, Franzén C, Taherzadeh M, Niklasson C, Liden G. Effects of furfural on the respiratory metabolism of Saccharomyces cerevisiae in glucose-limited chemostats. Appl Environ Microbiol. 2003;69:4076-86 pubmed..Thus, during both aerobic growth and anaerobic growth, the ability to tolerate furfural appears to be directly coupled to the ability to convert furfural to less inhibitory compounds...
- Hallberg M, Polozkov G, Hu G, Beve J, Gustafsson C, Ronne H, et al. Site-specific Srb10-dependent phosphorylation of the yeast Mediator subunit Med2 regulates gene expression from the 2-microm plasmid. Proc Natl Acad Sci U S A. 2004;101:3370-5 pubmed..Combined with previous studies on the effects of SRB10/SRB11 deletions, our data suggest that posttranslational modifications of Mediator subunits are important for regulation of gene expression. ..
- Lundin M, Baltscheffsky H, Ronne H. Yeast PPA2 gene encodes a mitochondrial inorganic pyrophosphatase that is essential for mitochondrial function. J Biol Chem. 1991;266:12168-72 pubmed..Fluorescence microscopy revealed that these cells have lost their mitochondrial DNA. We conclude that the mitochondrial PPase encoded by PPA2 is essential for mitochondrial function and maintenance of the mitochondrial genome. ..
- Gustafsson C, Samuelsson T. Mediator--a universal complex in transcriptional regulation. Mol Microbiol. 2001;41:1-8 pubmed..As an appendix to this review, we have created a database, MEDB, in which we have compiled information about all the S. cerevisiae Mediator subunits and their homologues in other eukaryotic cells (http://bio.lundberg.gu.se/medb/). ..
- Kota J, Melin Larsson M, Ljungdahl P, Forsberg H. Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae. Genetics. 2007;175:1681-94 pubmed
- Otero J, Vongsangnak W, Asadollahi M, Olivares Hernandes R, Maury J, Farinelli L, et al. Whole genome sequencing of Saccharomyces cerevisiae: from genotype to phenotype for improved metabolic engineering applications. BMC Genomics. 2010;11:723 pubmed publisher..The genome sequence, annotation, and a SNP viewer of CEN.PK113-7D are deposited at http://www.sysbio.se/cenpk. ..
- Berhe A, Zvyagilskaya R, Lagerstedt J, Pratt J, Persson B. Properties of the cysteine-less Pho84 phosphate transporter of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2001;287:837-42 pubmed
- Ranatunga W, Gakh O, Galeano B, Smith D, SÃ¶derberg C, Al Karadaghi S, et al. Architecture of the Yeast Mitochondrial Iron-Sulfur Cluster Assembly Machinery: THE SUB-COMPLEX FORMED BY THE IRON DONOR, Yfh1 PROTEIN, AND THE SCAFFOLD, Isu1 PROTEIN. J Biol Chem. 2016;291:10378-98 pubmed publisher..We suggest that this architecture is suitable to ensure concerted and protected transfer of potentially toxic iron and sulfur atoms to Isu1 during Fe-S cluster assembly. ..
- Carlsson A, Thomaeus S, Hamberg M, Stymne S. Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes. Eur J Biochem. 2004;271:2991-7 pubmed..The Delta6 double bond was efficiently further converted to an acetylenic bond by a second round of desaturation but only if the acyl substrate had a Delta12 double bond and that this was in the Z configuration. ..
- Forslid J, Bjorksten B, Hagersten K, Hed J. Erythrocyte-mediated scavenging of reactive oxygen metabolites generated by human polymorphonuclear leukocytes during phagocytosis: comparison between normal and Down's syndrome blood cells. Inflammation. 1989;13:543-51 pubmed..Our conclusion is that the increase of reactive oxygen metabolites scavengers in Down's syndrome blood cells is of benefit for the homeostasis between generated reactive oxygen metabolites and their propagation. ..
- Heessen S, Dantuma N, Tessarz P, Jellne M, Masucci M. Inhibition of ubiquitin/proteasome-dependent proteolysis in Saccharomyces cerevisiae by a Gly-Ala repeat. FEBS Lett. 2003;555:397-404 pubmed..The conserved effect of the GA repeat in yeast opens the possibility for the use of genetic screens to unravel its mode of action. ..
- Alikhani N, Berglund A, Engmann T, Spånning E, Vögtle F, Pavlov P, et al. Targeting capacity and conservation of PreP homologues localization in mitochondria of different species. J Mol Biol. 2011;410:400-10 pubmed publisher..Moreover, complementation studies in yeast show that native AtPreP restores the growth phenotype of yeast cells lacking Cym1, demonstrating functional conservation. ..
- Wikner C, Nilsson U, Meshalkina L, Udekwu C, Lindqvist Y, Schneider G. Identification of catalytically important residues in yeast transketolase. Biochemistry. 1997;36:15643-9 pubmed..The side chains of both residues interact with the C3 hydroxyl group of the donor substrate, and the results indicate that the two residues act in concert during proton abstraction of the C3 hydroxyl proton during catalysis. ..
- Wallberg A, Neely K, Hassan A, Gustafsson J, Workman J, Wright A. Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau1 activation domain. Mol Cell Biol. 2000;20:2004-13 pubmed..We also provide evidence that the SWI-SNF and SAGA complexes represent independent pathways of tau1-mediated activation but play overlapping roles that are able to compensate for one another under some conditions. ..
- Heessen S, Masucci M, Dantuma N. The UBA2 domain functions as an intrinsic stabilization signal that protects Rad23 from proteasomal degradation. Mol Cell. 2005;18:225-35 pubmed..Thus, the UBA2 domain of Rad23 is an evolutionarily conserved stabilization signal that allows Rad23 to interact with the proteasome without facing destruction. ..
- Swaminathan S, Masek T, Molin C, Pospisek M, Sunnerhagen P. Rck2 is required for reprogramming of ribosomes during oxidative stress. Mol Biol Cell. 2006;17:1472-82 pubmed..We infer that imbalances in the translational apparatus are a major reason for the inability of these cells to respond to stress. ..
- Lindberg L, Santos A, Riezman H, Olsson L, Bettiga M. Lipidomic profiling of Saccharomyces cerevisiae and Zygosaccharomyces bailii reveals critical changes in lipid composition in response to acetic acid stress. PLoS ONE. 2013;8:e73936 pubmed publisher..The results also suggest that acetic acid tolerance is associated with the ability of a given strain to generate large rearrangements in its lipid profile. ..
- Stolt A, Sasnauskas K, Koskela P, Lehtinen M, Dillner J. Seroepidemiology of the human polyomaviruses. J Gen Virol. 2003;84:1499-504 pubmed..The stability of the antibodies over time indicates that polyomavirus seropositivity is a valid marker of cumulative virus exposure, and polyoma VLP-based EIAs may therefore be useful for epidemiological studies of these viruses. ..
- Henricsson C, de Jesus Ferreira M, Hedfalk K, Elbing K, Larsson C, Bill R, et al. Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations. Appl Environ Microbiol. 2005;71:6185-92 pubmed
- Gowda N, Kaimal J, Masser A, Kang W, FriedlÃ¤nder M, AndrÃ©asson C. Cytosolic splice isoform of Hsp70 nucleotide exchange factor Fes1 is required for the degradation of misfolded proteins in yeast. Mol Biol Cell. 2016;27:1210-9 pubmed publisher..This study provides key findings for the understanding of the organization of protein quality control mechanisms in the cytosol and nucleus. ..
- Aström S, Byström A. Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination. Cell. 1994;79:535-46 pubmed..The modification enzyme was shown to recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs. ..
- Fristedt U, Weinander R, Martinsson H, Persson B. Characterization of purified and unidirectionally reconstituted Pho84 phosphate permease of Saccharomyces cerevisiae. FEBS Lett. 1999;458:1-5 pubmed..Site-specific proteolysis of the immunoreactive N-terminal sequence in the reconstituted protein suggests a unidirectional insertion into liposomes. ..
- Kuzmenko A, Derbikova K, Salvatori R, Tankov S, Atkinson G, Tenson T, et al. Aim-less translation: loss of Saccharomyces cerevisiae mitochondrial translation initiation factor mIF3/Aim23 leads to unbalanced protein synthesis. Sci Rep. 2016;6:18749 pubmed publisher..Our results provide one more example of deviation of mitochondrial translation from its bacterial origins. ..
- Odegrip R, Schoen S, Haggård Ljungquist E, Park K, Chattoraj D. The interaction of bacteriophage P2 B protein with Escherichia coli DnaB helicase. J Virol. 2000;74:4057-63 pubmed
- Verhoef L, Heinen C, Selivanova A, Halff E, Salomons F, Dantuma N. Minimal length requirement for proteasomal degradation of ubiquitin-dependent substrates. FASEB J. 2009;23:123-33 pubmed publisher..These observations provide an explanation for the accumulation of UBB(+1) in neurodegenerative disorders and offers new insights into the physical constraints determining proteasomal degradation. ..
- Nandy S, Jouhten P, Nielsen J. Reconstruction of the yeast protein-protein interaction network involved in nutrient sensing and global metabolic regulation. BMC Syst Biol. 2010;4:68 pubmed publisher..cerevisiae. Furthermore, we propose this annotated reconstruction as a first step towards generation of an extensive annotated protein-protein interaction network of signal transduction and metabolic regulation in this yeast. ..
- Pei Z, Anderson H, Aastrup T, Ramström O. Study of real-time lectin-carbohydrate interactions on the surface of a quartz crystal microbalance. Biosens Bioelectron. 2005;21:60-6 pubmed..18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol...
- Olsson I, Berrez J, Leipus A, Ostlund C, Mutvei A. The arginine methyltransferase Rmt2 is enriched in the nucleus and co-purifies with the nuclear porins Nup49, Nup57 and Nup100. Exp Cell Res. 2007;313:1778-89 pubmed..In addition, a genome-wide transcription study of the rmt2Delta mutant shows significant downregulation of the transcription of MYO1, encoding the Type II myosin heavy chain required for cytokinesis and cell separation. ..
- Jacobson T, Navarrete C, Sharma S, Sideri T, Ibstedt S, Priya S, et al. Arsenite interferes with protein folding and triggers formation of protein aggregates in yeast. J Cell Sci. 2012;125:5073-83 pubmed publisher..Our findings describe a novel mechanism of toxicity that may explain the suggested role of this metalloid in the etiology and pathogenesis of protein folding disorders associated with arsenic poisoning. ..
- Alriksson B, Horváth I, Sjöde A, Nilvebrant N, Jönsson L. Ammonium hydroxide detoxification of spruce acid hydrolysates. Appl Biochem Biotechnol. 2005;121-124:911-22 pubmed..The results strongly suggest that the highly positive effects of NH4OH treatments are owing to chemical conversions rather than stimulation of the yeast cells by ammonium ions during the fermentation. ..
- Sabouri N, Viberg J, Goyal D, Johansson E, Chabes A. Evidence for lesion bypass by yeast replicative DNA polymerases during DNA damage. Nucleic Acids Res. 2008;36:5660-7 pubmed publisher..The nucleotide inserted opposite 8-oxoG is dATP. We propose that during DNA damage in S. cerevisiae increased dNTP concentration allows replicative DNA polymerases to bypass certain DNA lesions. ..
- Lundh F, Mouillon J, Samyn D, Stadler K, Popova Y, Lagerstedt J, et al. Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter. Biochemistry. 2009;48:4497-505 pubmed publisher..Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown. ..
- Tyo K, Liu Z, Petranovic D, Nielsen J. Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress. BMC Biol. 2012;10:16 pubmed publisher..This molecular insight has direct implications on engineering a broad range of recombinant proteins for secretion and provides potential hypotheses for the root causes of several secretory-associated diseases. ..
- Boban M, Zargari A, Andréasson C, Heessen S, Thyberg J, Ljungdahl P. Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors. J Cell Biol. 2006;173:695-707 pubmed..These findings reveal an unanticipated role of inner nuclear membrane proteins in controlling gene expression. ..
- Pfirrmann T, Heessen S, Omnus D, Andréasson C, Ljungdahl P. The prodomain of Ssy5 protease controls receptor-activated proteolysis of transcription factor Stp1. Mol Cell Biol. 2010;30:3299-309 pubmed publisher..Our results define a regulatory mechanism that is novel for eukaryotic proteases functioning within cells. ..
- Otero J, Cimini D, Patil K, Poulsen S, Olsson L, Nielsen J. Industrial systems biology of Saccharomyces cerevisiae enables novel succinic acid cell factory. PLoS ONE. 2013;8:e54144 pubmed publisher..cerevisiae, and hence show proof of concept that this is a potentially attractive cell factory for over-producing different platform chemicals. ..
- Söderberg C, Gillam M, Ahlgren E, Hunter G, Gakh O, Isaya G, et al. The Structure of the Complex between Yeast Frataxin and Ferrochelatase: CHARACTERIZATION AND PRE-STEADY STATE REACTION OF FERROUS IRON DELIVERY AND HEME SYNTHESIS. J Biol Chem. 2016;291:11887-98 pubmed publisher..Furthermore, they support the proposal that frataxin-mediated delivery of this potentially toxic substrate overcomes formation of reactive oxygen species. ..
- Jessop Fabre M, Jakočiūnas T, Stovícek V, Dai Z, Jensen M, Keasling J, et al. EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016;11:1110-7 pubmed publisher..The publicly available EasyClone-MarkerFree vector suite allows for facile and highly standardized genome engineering, and should be of particular interest to researchers working on yeast chassis with limited markers available. ..
- Berhe A, Fristedt U, Persson B. Expression and purification of the high-affinity phosphate transporter of Saccharomyces cerevisiae. Eur J Biochem. 1995;227:566-72 pubmed..Approximately 5 mg purified His10-permease was obtained from 3 g (wet mass) cells. The purified phosphate permease chimera catalyzes uncoupler-sensitive phosphate transport after reconstitution into proteoliposomes. ..
- Forsberg H, Ljungdahl P. Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol. 2001;21:814-26 pubmed..These results reveal the dynamic nature of the amino acid-initiated signals transduced by the SPS sensor. ..
- Ansell R, Granath K, Hohmann S, Thevelein J, Adler L. The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J. 1997;16:2179-87 pubmed..We conclude that expression of GPD2 is controlled by a novel, oxygen-independent, signalling pathway which is required to regulate metabolism under anoxic conditions. ..
- Provost P, Samuelsson B, Radmark O. Interaction of 5-lipoxygenase with cellular proteins. Proc Natl Acad Sci U S A. 1999;96:1881-5 pubmed..The identification of these 5LO-interacting proteins provides additional approaches to studies of the cellular functions of 5LO. ..
- Chabes A, Domkin V, Thelander L. Yeast Sml1, a protein inhibitor of ribonucleotide reductase. J Biol Chem. 1999;274:36679-83 pubmed..However, the inhibition observed in an in vitro mouse ribonucleotide reductase assay is less pronounced than the inhibition in yeast and probably occurs via a different mechanism. ..
- Albers E, Laizé V, Blomberg A, Hohmann S, Gustafsson L. Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae. J Biol Chem. 2003;278:10264-72 pubmed..Moreover, since phosphoglycerate dehydrogenase activity requires NAD(+) as cofactor, deletion of SER3 and SER33 markedly affected redox metabolism as shown by substrate and product analysis. ..
- Ott M, Norberg E, Walter K, Schreiner P, Kemper C, Rapaport D, et al. The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. J Biol Chem. 2007;282:27633-9 pubmed..Here, we show that the protein translocase of the outer mitochondrial membrane is required for Bax insertion and cytochrome c release. ..
- Laadan B, Almeida J, Radstrom P, Hahn Hägerdal B, Gorwa Grauslund M. Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae. Yeast. 2008;25:191-8 pubmed publisher..Increased biomass production and HMF conversion rate were achieved in a CEN.PK S. cerevisiae strain overexpressing the mutated ADH1 gene. ..
- Ye T, Elbing K, Hohmann S. The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulation. Microbiology. 2008;154:2814-26 pubmed publisher..Snf1p appears to be part of a wider functional network than previously anticipated and the full complexity of this network remains to be elucidated. ..
- Carter S, Vigasová D, Chen J, Chovanec M, Aström S. Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism. Proc Natl Acad Sci U S A. 2009;106:12037-42 pubmed publisher..This unexpected link between NHEJ and HR components may represent cross-talk between DSB repair pathways to ensure efficient repair. ..
- Saaf A, Monne M, de Gier J, von Heijne G. Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli. J Biol Chem. 1998;273:30415-8 pubmed..In contrast to partial N-terminal fusion protein constructs, the full-length protein folds into a protease-resistant conformation, suggesting that important folding determinants are present in the C-terminal part of the molecule. ..
- Nordlund M, Johansson J, von Pawel Rammingen U, Byström A. Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae. RNA. 2000;6:844-60 pubmed..The purified yNucR endo-exonuclease has been reported to have an NH2-D-E-K-N-L motif, which is not found in the Trm2p. Therefore, we suggest that the yNucR endo-exonuclease is encoded by a gene other than TRM2. ..
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- de Jong B, Siewers V, Nielsen J. Physiological and transcriptional characterization of Saccharomyces cerevisiae engineered for production of fatty acid ethyl esters. FEMS Yeast Res. 2016;16:fov105 pubmed publisher..In conclusion, our analysis clearly shows that engineering of fatty acid biosynthesis results in transcriptional reprogramming and has a significant effect on overall cellular metabolism. ..
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