Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Japan
Topic: saccharomyces cerevisiae
Publications239 found, 100 shown here
- Nakamura H, Miura K, Fukuda Y, Shibuya I, Ohta A, Takagi M. Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2000;64:167-72 pubmed..These results indicate that PS synthesis is required for the maximal tryptophan-transporting activity of S. cerevisiae at low tryptophan concentrations...
- Kobayashi A, Miyake T, Ohyama Y, Kawaichi M, Kokubo T. Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae. J Biol Chem. 2001;276:395-405 pubmed
- Cui Z, Horecka J, Jigami Y. Cdc4 is involved in the transcriptional control of OCH1, a gene encoding alpha-1,6-mannosyltransferase in Saccharomyces cerevisiae. Yeast. 2002;19:69-77 pubmed..Interestingly, cdc4(bon) and Delta swi4 show a synthetic growth defect when combined. ..
- Sakurai H, Ota A. Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: importance in normal cell growth, stress resistance, and longevity. FEBS Lett. 2011;585:2744-8 pubmed publisher..Therefore, HSF-regulated changes in expression of these chaperone genes are necessary to maintain cell viability under various growth conditions. ..
- Nishikiori M, Sugiyama S, Xiang H, Niiyama M, Ishibashi K, Inoue T, et al. Crystal structure of the superfamily 1 helicase from Tomato mosaic virus. J Virol. 2012;86:7565-76 pubmed publisher..Our results provide a structural basis of viral superfamily 1 helicases...
- Suzuki T, Iwahashi Y. Acetylated Deoxynivalenol Generates Differences of Gene Expression that Discriminate Trichothecene Toxicity. Toxins (Basel). 2016;8:42 pubmed publisher..3ADON also induced a different expression trend in these genes than DON and 15ADON. These differences in gene expression suggest that DON and its derivatives have different effects on cells. ..
- Fujita A, Misumi Y, Ikehara Y, Kobayashi H. The yeast SFL2 gene may be necessary for mating-type control. Gene. 1992;112:85-90 pubmed..However, there is no significant difference in the levels of the MAT alpha 2 and MATa1 transcripts. These results suggest that the SFL2 gene product may be necessary for alpha 2 and a1-alpha 2 repression. ..
- Utsugi T, Toh e A, Kikuchi Y. A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochim Biophys Acta. 1995;1263:285-8 pubmed..The predicted gene product (29,999 Da) is basic and partially homologous to various histone H1. The level of the gene expression increased 2-fold when exposed to mating pheromone. ..
- Yoko o T, Kato H, Matsui Y, Takenawa T, Toh e A. Isolation and characterization of temperature-sensitive plc1 mutants of the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1995;247:148-56 pubmed..At the restrictive temperature, plc1 mutant cells ceased growth at random times during the cell cycle, a result that suggests that PLC1 is required at several or all stages of the cell cycle. ..
- Habu T, Taki T, West A, Nishimune Y, Morita T. The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res. 1996;24:470-7 pubmed..Since the alternatively spliced Dmc1-d transcript was detected in both male and female germ cells, the encoded protein DMC1-D may have a novel role in mammalian genetic recombination in meiosis. ..
- Nozaki T, Nishimura K, Michael A, Maruyama T, Kakinuma Y, Igarashi K. A second gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1996;228:452-8 pubmed..The results indicate that polyamine transport of yeast is regulated by multiple phosphorylation/dephosphorylation pathways. ..
- Ohtoshi A, Miyake T, Arai K, Masai H. Analyses of Saccharomyces cerevisiae Cdc7 kinase point mutants: dominant-negative inhibition of DNA replication on overexpression of kinase-negative Cdc7 proteins. Mol Gen Genet. 1997;254:562-70 pubmed..Our results are consistent with the notion that association of Dbf4 with Cdc7 is essential for the G1 to S transition in S. cerevisiae. ..
- Matsuura A, Tsukada M, Wada Y, Ohsumi Y. Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae. Gene. 1997;192:245-50 pubmed..We found overall homology of Apglp with C. elegans Unc-51 protein, suggesting that homologous molecular mechanisms, conserved from unicellular to multicellular organisms, are involved in dynamic membrane flow. ..
- Ueda T, Yoshizumi T, Anai T, Matsui M, Uchimiya H, Nakano A. AtGDI2, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor. Gene. 1998;206:137-43 pubmed..Genomic DNA hybridization using specific probes reveals the presence of one more GDI gene in Arabidopsis. This may imply differentiated roles of GDI in higher plants. ..
- Nakayama K, Feng Y, Tanaka A, Jigami Y. The involvement of mnn4 and mnn6 mutations in mannosylphosphorylation of O-linked oligosaccharide in yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 1998;1425:255-62 pubmed..The amount of mannosylphosphorylated mannotriose was 7% of total O-linked oligosaccharides (20% of neutral mannotriose) of chitinase in strain mnn1. ..
- Tohe A, Oguchi T. Isolation and characterization of the yeast las21 mutants, which are sensitive to a local anestheticum, tetracaine. Genes Genet Syst. 1998;73:365-75 pubmed..We found some genetic interactions between LAS21 and Ras/cAMP cascade genes. These results suggest that LAS21 defines unknown pathway regulating the stress response of yeast. ..
- Ozaki T, Hanaoka E, Naka M, Nakagawara A, Sakiyama S. Cloning and characterization of rat BAT3 cDNA. DNA Cell Biol. 1999;18:503-12 pubmed..In addition, the level of BAT3 mRNA expression was more downregulated in some of the transformed cells, including v-mos- and v-Ha-ras-transformed 3Y1 cells, than in the parental cells. ..
- Tauchi H. Positional cloning and functional analysis of the gene responsible for Nijmegen breakage syndrome, NBS1. J Radiat Res. 2000;41:9-17 pubmed..Functional analysis of the NBS1 protein is in progress and it should provide further clues to understanding the repair mechanism of radiation-induced DNA double-strand breaks. ..
- Tsuchiya E, Yukawa M, Miyakawa T, Kimura K, Takahashi H. Borrelidin inhibits a cyclin-dependent kinase (CDK), Cdc28/Cln2, of Saccharomyces cerevisiae. J Antibiot (Tokyo). 2001;54:84-90 pubmed..Although the inhibition of CDK activity may not be a solo cause of the G1 arrest, our results indicate that borrelidin is a potential lead compound for developing novel CDK inhibitors of higher eukaryotes. ..
- Koyama H, Itoh M, Miyahara K, Tsuchiya E. Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae. FEBS Lett. 2002;531:215-21 pubmed..These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage. ..
- Imazu H, Sakurai H. Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryot Cell. 2005;4:1050-6 pubmed..Several of the other suppressors were found to encode proteins functioning in cell wall organization. These results suggest that Hsf1 in concert with Pkc1 regulates cell wall remodeling in response to heat shock. ..
- Morohashi N, Yamamoto Y, Kuwana S, Morita W, Shindo H, Mitchell A, et al. Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome. Eukaryot Cell. 2006;5:1925-33 pubmed..Our results illustrate a useful paradigm for analysis of chromatin structural effects at genomic loci. ..
- Hirayama H, Fujita M, Yoko o T, Jigami Y. O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae. J Biochem. 2008;143:555-67 pubmed publisher..Thus, we propose that O-mannosylation by Pmt1p and Pmt2p might be a key step in the targeting of some misfolded proteins for degradation via the proteasome-dependent ERAD pathway. ..
- Hase T, Niimura Y, Tanaka H. Difference in gene duplicability may explain the difference in overall structure of protein-protein interaction networks among eukaryotes. BMC Evol Biol. 2010;10:358 pubmed publisher..These results suggest that disassortative structures observed in PINs are merely a byproduct of preferential duplications of low-degree genes, which might be caused by an organism's living environment. ..
- Nosaka K, Esaki H, Onozuka M, Konno H, Hattori Y, Akaji K. Facilitated recruitment of Pdc2p, a yeast transcriptional activator, in response to thiamin starvation. FEMS Microbiol Lett. 2012;330:140-7 pubmed publisher..On the other hand, the association of Pdc2p with PDC5 was unaffected by thiamin. We also identified a DNA element in the upstream region of PDC5, which can bind to Pdc2p and is required for the expression of PDC5. ..
- Parui P, Deshpande M, Nagao S, Kamikubo H, Komori H, Higuchi Y, et al. Formation of oligomeric cytochrome c during folding by intermolecular hydrophobic interaction between N- and C-terminal ?-helices. Biochemistry. 2013;52:8732-44 pubmed publisher..These results show that it is important to consider formation of domain-swapped oligomeric proteins when folding at high protein concentrations. ..
- Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M. The signaling pathways underlying starvation-induced upregulation of Î±-mannosidase Ams1 in Saccharomyces cerevisiae. Biochim Biophys Acta. 2016;1860:1192-201 pubmed publisher..The signaling molecules responsible for regulation of Ams1 were also clarified. ..
- Abe T, Kobayashi M, Okawa Y, Inui T, Yoshida J, Higashio H, et al. Yeast Ca(2+)-signal transduction inhibitors isolated from Dominican amber prevent the degranulation of RBL-2H3 cells through the inhibition of Ca(2+)-influx. Fitoterapia. 2016;113:188-94 pubmed publisher..The compounds having the growth restoring activity against the mutant yeast have potential as anti-allergic compounds. ..
- Mochizuki N, Yamamoto M. Reduction in the intracellular cAMP level triggers initiation of sexual development in fission yeast. Mol Gen Genet. 1992;233:17-24 pubmed..Disruption of pde1 made S. pombe cells partially sterile and meiosis-deficient, indicating that this cAMP phosphodiesterase plays an important role in balancing the cAMP level in vivo. ..
- Yamaki H, Yamaguchi M, Tsuruo T, Yamaguchi H. Mechanism of action of an antifungal antibiotic, RI-331, (S) 2-amino-4-oxo-5-hydroxypentanoic acid; kinetics of inactivation of homoserine dehydrogenase from Saccharomyces cerevisiae. J Antibiot (Tokyo). 1992;45:750-5 pubmed..Since such enzymic activity is not present in animal cells, the selective antifungal activity of the antibiotic is thus explained. ..
- Kitamura K, Shimoda C. The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein. EMBO J. 1991;10:3743-51 pubmed..The mam2 gene was also transcribed in h+/h- diploid strains. The fact that the map1/mam2 homozygous diploid cells are incapable of sporulation implies that the pheromone signalling system is necessary for sporulation in diploid cells. ..
- Shirayama M, Matsui Y, Tanaka K, Toh e A. Isolation of a CDC25 family gene, MSI2/LTE1, as a multicopy suppressor of ira1. Yeast. 1994;10:451-61 pubmed..These results suggest that MSI2 is involved in the termination of M phase and that this process is regulated by a ras superfamily gene product. ..
- Fujimura K, Tanaka K, Toh e A. A dominant interfering mutation in RAS1 of Saccharomyces cerevisiae. Mol Gen Genet. 1993;241:280-6 pubmed..These results suggest that the RAS1Ser22 gene product interferes with the normal interaction of Ras with Cdc25 by forming a dead-end complex between Ras1Ser22 and Cdc25 proteins. ..
- Yoshida S, Ohya Y, Goebl M, Nakano A, Anraku Y. A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae. J Biol Chem. 1994;269:1166-72 pubmed..We conclude that STT4 encodes a yeast PI4-kinase that functions in the PKC1 protein kinase pathway. ..
- Morita T, Yoshimura Y, Yamamoto A, Murata K, Mori M, Yamamoto H, et al. A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes. Proc Natl Acad Sci U S A. 1993;90:6577-80 pubmed..This gene is expressed in the thymus, testis, ovary, spleen, and intestine, suggesting that its product is involved in mitotic and meiotic recombination in addition to DNA repair. ..
- Ogawa N, Saitoh H, Miura K, Magbanua J, Bun Ya M, Harashima S, et al. Structure and distribution of specific cis-elements for transcriptional regulation of PHO84 in Saccharomyces cerevisiae. Mol Gen Genet. 1995;249:406-16 pubmed..The UAS function of the GCACGTTTT motif with respect to the Pi signal depends on its orientation in the promoter sequence. ..
- Miyahara K, Hirata D, Miyakawa T. yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae. Curr Genet. 1996;29:103-5 pubmed..A sequence similar to the yAP-1 recognition element recently identified in the stress-responsive yeast genes was found in this region and in the 5'-flanking sequences of SNQ2...
- Uemura H, Pandit S, Jigami Y, Sternglanz R. Mutations in GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, suppress the temperature-sensitive growth of hpr1 mutants. Genetics. 1996;142:1095-103 pubmed..Plasmid DNA isolated from gcr3 mutants was significantly more negatively supercoiled than normal, suggesting that Gcr3 protein, like topoisomerase I and Hpr1p, affects chromatin structure, perhaps during transcription. ..
- Tsuchiya E, Matsuzaki G, Kurano K, Fukuchi T, Tsukao A, Miyakawa T. The Saccharomyces cerevisiae SSD1 gene is involved in the tolerance to high concentration of Ca2+ with the participation of HST1/NRC1/BFR1. Gene. 1996;176:35-8 pubmed..The results indicated the possibility that the SSD1 gene is involved in the tolerance mechanism to high concentration of Ca2+, and the HST1 gene participates with SSD1 by its functional redundancy in Ca2+ tolerance. ..
- Itoh K, Nakamura K, Kimura T, Itoh S, Kamataki T. Molecular cloning of mouse liver flavin containing monooxygenase (FMO1) cDNA and characterization of the expression product: metabolism of the neurotoxin, 1,2,3,4-tetrahydroisoquinoline (TIQ). J Toxicol Sci. 1997;22:45-56 pubmed..The K(m) values for chlorpromazine, imipramine and TIQ were determined to be 2,4, 16.0, 435 mM, respectively. This is the first report to show that an expressed FMO can metabolize a neurotoxin, TIQ. ..
- Kume K, Shimizu T. cDNA cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase. Biochem Biophys Res Commun. 1997;237:663-6 pubmed..The enzyme utilized both saturated and unsaturated acyl-CoA as an acyl-donor, while it utilized LPA but not other lysophospholipids as an acceptor. ..
- Kagami M, Toh e A, Matsui Y. SRO9, a multicopy suppressor of the bud growth defect in the Saccharomyces cerevisiae rho3-deficient cells, shows strong genetic interactions with tropomyosin genes, suggesting its role in organization of the actin cytoskeleton. Genetics. 1997;147:1003-16 pubmed..Unlike tropomyosin, Sro9p does not colocalize with actin cables but is diffusely cytoplasmic. These results suggest that Sro9p is a new cytoplasmic factor involved in the organization of actin filaments. ..
- Hashimoto H, Yoda K. Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus. Biochem Biophys Res Commun. 1997;241:682-6 pubmed..These complexes with similar but partially different constituents may represent essential parts of glycosylation machinery in the yeast Golgi compartments. ..
- Tanimura S, Ohtsuka S, Mitsui K, Shirouzu K, Yoshimura A, Ohtsubo M. MDM2 interacts with MDMX through their RING finger domains. FEBS Lett. 1999;447:5-9 pubmed..Interaction of MDMX with MDM2 through the C-terminal RING finger domains resulted in inhibiting degradation of MDM2. These data indicate that MDMX functions as a regulator of MDM2. ..
- Sasaki T, Toh e A, Kikuchi Y. Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Mol Gen Genet. 2000;262:940-8 pubmed..Most of the isolated tmr mutations rescued one of the defects seen in both types of heat stress response in the tom1 mutant. ..
- Yoshida S, Anraku Y. Characterization of staurosporine-sensitive mutants of Saccharomyces cerevisiae: vacuolar functions affect staurosporine sensitivity. Mol Gen Genet. 2000;263:877-88 pubmed
- Toh e A, Oguchi T. Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst. 2002;77:309-22 pubmed..Las21 did not show preference for the metabolic pathway to produce phosphatidylethanolamine. ..
- Fukuda T, Ohya Y. Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE (PI-SceI). Mol Genet Genomics. 2006;275:204-14 pubmed
- Sekine T, Kawaguchi A, Hamano Y, Takagi H. Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance. Appl Environ Microbiol. 2007;73:4011-9 pubmed..Furthermore, we found that yeast cells expressing Ile150Thr and Asn142Asp/Ile166Val mutant GKs were more tolerant to freezing stress than cells expressing the Asp154Asn mutant. ..
- Kamisaka Y, Tomita N, Kimura K, Kainou K, Uemura H. DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae. Biochem J. 2007;408:61-8 pubmed..The strains obtained have a lipid content that is high enough to act as a model of oleaginous yeast and they may be useful for the metabolic engineering of lipid production in yeast. ..
- Yukawa M, Yo K, Hasegawa H, Ueno M, Tsuchiya E. The Rpd3/HDAC complex is present at the URS1 cis-element with hyperacetylated histone H3. Biosci Biotechnol Biochem. 2009;73:378-84 pubmed..The biological significance of this phenomenon is discussed below. ..
- Saegusa S, Totsuka M, Kaminogawa S, Hosoi T. Saccharomyces cerevisiae and Candida albicans stimulate cytokine secretion from human neutrophil-like HL-60 cells differentiated with retinoic acid or dimethylsulfoxide. Biosci Biotechnol Biochem. 2009;73:2600-8 pubmed..cerevisiae or C. albicans. Our results suggest that both intact and heat-killed S. cerevisiae and C. albicans induce cytokine responses of neutrophils in the intestine, and stimulate host immune function. ..
- Ito T, Koga K, Hemmi H, Yoshimura T. Role of zinc ion for catalytic activity in d-serine dehydratase from Saccharomyces cerevisiae. FEBS J. 2012;279:612-24 pubmed publisher..Our data suggest that DsdSC catalyzes the ?-hydrogen abstraction and hydroxyl group elimination in a concerted fashion. ..
- Ogata T. Nitrogen starvation induces expression of Lg-FLO1 and flocculation in bottom-fermenting yeast. Yeast. 2012;29:487-94 pubmed publisher..This suggests that the flocculation of bottom-fermenting yeast is under the control of a nitrogen catabolite repression (NCR)-like mechanism. ..
- Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, et al. X-ray crystal structure of voltage-gated proton channel. Nat Struct Mol Biol. 2014;21:352-7 pubmed publisher..These findings provide a platform for understanding the general principles of voltage sensing and proton permeation. ..
- Usami Y, Uemura S, Mochizuki T, Morita A, Shishido F, Inokuchi J, et al. Functional mapping and implications of substrate specificity of the yeast high-affinity leucine permease Bap2. Biochim Biophys Acta. 2014;1838:1719-29 pubmed publisher..This result suggests that the substrate partition efficiency to the buried Bap2 binding pocket is the primary determinant of substrate specificity rather than structural amino acid side chain recognition. ..
- Suzuki H, Hosokawa Y, Toda H, Nishikimi M, Ozawa T. Cloning and sequencing of a cDNA for human mitochondrial ubiquinone-binding protein of complex III. Biochem Biophys Res Commun. 1988;156:987-94 pubmed..This implies that the human QP-C is synthesized without a presequence which is required for import of most nuclear-encoded mitochondrial proteins into mitochondria. ..
- Miyamoto Y, Machida K, Mizunuma M, Emoto Y, Sato N, Miyahara K, et al. Identification of Saccharomyces cerevisiae isoleucyl-tRNA synthetase as a target of the G1-specific inhibitor Reveromycin A. J Biol Chem. 2002;277:28810-4 pubmed..IleRS inhibition by RM-A led to transcriptional activation of the ILS1 gene via the Gcn2-Gcn4 general amino acid control pathway, and this autoregulation seemed to contribute to RM-A resistance. ..
- Fujita M, Umemura M, Yoko o T, Jigami Y. PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins. Mol Biol Cell. 2006;17:5253-64 pubmed
- Hirayama H, Suzuki T. Metabolism of free oligosaccharides is facilitated in the och1? mutant of Saccharomyces cerevisiae. Glycobiology. 2011;21:1341-8 pubmed publisher..Up-regulation of Ams1 activity was also apparent for cells treated with cell wall perturbation reagent. These results provide an insight into a possible link between catabolism of fOSs and cell wall stress. ..
- Takeuchi T, Miyahara K, Hirata D, Miyakawa T. Mutational analysis of Yap1 protein, an AP-1-like transcriptional activator of Saccharomyces cerevisiae. FEBS Lett. 1997;416:339-43 pubmed..The carboxy-terminal domain of Yap1 appears to act negatively in cadmium resistance. ..
- Yamada Okabe T, Mio T, Matsui M, Kashima Y, Arisawa M, Yamada Okabe H. Isolation and characterization of the Candida albicans gene for mRNA 5'-triphosphatase: association of mRNA 5'-triphosphatase and mRNA 5'-guanylyltransferase activities is essential for the function of mRNA 5'-capping enzyme in vivo. FEBS Lett. 1998;435:49-54 pubmed
- Mori T, Miura K, Fujiwara T, Shin S, Inazawa J, Nakamura Y. Isolation and mapping of a human gene (DIFF6) homologous to yeast CDC3, CDC10, CDC11, and CDC12, and mouse Diff6. Cytogenet Cell Genet. 1996;73:224-7 pubmed..5 kb long, was expressed ubiquitously in all human tissues examined, but a 2.0-kb alternative transcript lacking any long AU-rich element in the 3' non-coding region was expressed abundantly only in testis, heart and skeletal muscle. ..
- Sakurai H, Fukasawa T. Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE. Genes Cells. 2003;8:41-50 pubmed..Our results strongly suggest that the TFIIE requirement of a gene is determined by a target(s) in Mediator through which the signal of the cognate activator is transmitted. ..
- Funakoshi M, Kajiwara R, Goda T, Nishimoto T, Kobayashi H. Isolation and characterisation of a mutation in the PMR1 gene encoding a Golgi membrane ATPase, which causes hypersensitivity to over-expression of Clb3 in Saccharomyces cerevisiae. Mol Gen Genet. 2000;264:29-36 pubmed..On induction of Clb3 expression in the pmr1-1 strain, the cells arrested at anaphase with an elongated daughter bud. We discuss possible interpretations of this synthetic lethal phenotype. ..
- Shinkyo R, Sakaki T, Ohta M, Inouye K. Metabolic pathways of dioxin by CYP1A1: species difference between rat and human CYP1A subfamily in the metabolism of dioxins. Arch Biochem Biophys. 2003;409:180-7 pubmed..The metabolic pathways contain most of the metabolites observed in vivo using experimental animals, suggesting that P450 monooxygenase systems including CYP1A1 are greatly responsible for dioxin metabolism in vivo. ..
- Hayashi N, Kobayashi M, Shimizu H, Yamamoto K, Murakami S, Nishimoto T. Mutations in Ran system affected telomere silencing in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2007;363:788-94 pubmed..Furthermore, hyperphosphorylated Sir3 protein accumulated in the rna1-1 mutant. These results suggest that RanGAP is required for the heterochromatin structure at the telomere in budding yeast. ..
- Yamochi W, Tanaka K, Nonaka H, Maeda A, Musha T, Takai Y. Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae. J Cell Biol. 1994;125:1077-93 pubmed..These results suggest that Rho1 small GTP-binding protein binds to a specific site at the growth region of cells, where Rho1p exerts its function in controlling cell growth. ..
- Irie K, Takase M, Araki H, Oshima Y. A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein. Mol Gen Genet. 1993;236:283-8 pubmed..The SMP2 locus was mapped at a site 71 cM from lys7 and 21 cM from ilv2/SMR1 on the right arm of chromosome XIII. ..
- Aso T, Conrad M. Molecular cloning of DNAs encoding the regulatory subunits of elongin from Saccharomyces cerevisiae and Drosophila melanogaster. Biochem Biophys Res Commun. 1997;241:334-40 pubmed..The expression of yeast Elongin C mRNA is dramatically upregulated during sporulation; however, the gene is not essential for sporulation and viability in yeast cell. ..
- Akiyoshi Y, Clayton J, Phan L, Yamamoto M, Hinnebusch A, Watanabe Y, et al. Fission yeast homolog of murine Int-6 protein, encoded by mouse mammary tumor virus integration site, is associated with the conserved core subunits of eukaryotic translation initiation factor 3. J Biol Chem. 2001;276:10056-62 pubmed..We propose that Int6 is not an essential subunit of eIF3 but might be involved in regulating the activity of eIF3 for translation of specific mRNAs in S. pombe. ..
- Arikawa Y, Enomoto K, Muratsubaki H, Okazaki M. Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth. FEMS Microbiol Lett. 1998;165:111-6 pubmed..These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions. ..
- Shobuike T, Sugano S, Yamashita T, Ikeda H. Cloning and characterization of mouse Dhm2 cDNA, a functional homolog of budding yeast SEP1. Gene. 1997;191:161-6 pubmed..Northern analysis revealed that 5.8 kb mRNA corresponding to Dhm2 open reading frame is produced highly in testis. These results strongly suggest that Dhm2p participates in gametogenesis in mouse. ..
- Momose Y, Iwahashi H. Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium. Environ Toxicol Chem. 2001;20:2353-60 pubmed..Based on these results, we concluded that DNA microarrays are very useful instruments for creating new bioassay systems and finding genetic promoters of stress indicators. ..
- Fujita K, Matsuyama A, Kobayashi Y, Iwahashi H. The genome-wide screening of yeast deletion mutants to identify the genes required for tolerance to ethanol and other alcohols. FEMS Yeast Res. 2006;6:744-50 pubmed..A global screening approach of yeast deletion library mutants was useful in elucidating the mechanisms of alcohol tolerance based on different lipophilicities. ..
- Takayama K, Suye S, Kuroda K, Ueda M, Kitaguchi T, Tsuchiyama K, et al. Surface display of organophosphorus hydrolase on Saccharomyces cerevisiae. Biotechnol Prog. 2006;22:939-43 pubmed
- Nakamura Y, Ito K, Isaksson L. Emerging understanding of translation termination. Cell. 1996;87:147-50 pubmed
- Kato T, Matsuda T, Matsui S, Mizutani T, Saeki K. Activation of the aryl hydrocarbon receptor by methyl yellow and related congeners: structure-activity relationships in halogenated derivatives. Biol Pharm Bull. 2002;25:466-71 pubmed..These results suggest that the amide bond is equivalent to the -N=N- or -CH=CH- double bond for recognition as the ligand by AhR in 1,2-diphenyl-1,2-ene derivatives...
- Araki T, Uesono Y, Oguchi T, Toh e A. LAS24/KOG1, a component of the TOR complex 1 (TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast. Genes Genet Syst. 2005;80:325-43 pubmed..Consistent with the broad cellular functions exerted by the TOR pathway, we found that Las24p was associated with membranes and was localized at vacuoles, the plasma membrane and small vesicles. ..
- Seki T, Tada S, Katada T, Enomoto T. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL. Biochem Biophys Res Commun. 1997;234:48-53 pubmed..In addition, interaction of the putative NLS in DNA helicase Q1 with Qip1 and Rch1 but not with hSrp1 was confirmed by the two-hybrid system. ..
- Nakayama K, Nagasu T, Shimma Y, Kuromitsu J, Jigami Y. OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides. EMBO J. 1992;11:2511-9 pubmed..Finally, the OCH1 gene was found to encode a novel mannosyltransferase which specifically transfers [14C]mannose to the unique acceptor, the core-like oligosaccharide of cell wall mannan accumulated in the och1 disruptant. ..
- Yoshida S, Ohya Y, Hirose R, Nakano A, Anraku Y. STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway. Gene. 1995;160:117-22 pubmed..Genetic studies of yeast mutants carrying delta stt10, delta bck1, stt1/pkc1 or stt4 have revealed that their functions are phenotypically related to maintenance of cellular osmotic integrity. ..
- Johzuka K, Ogawa H. Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae. Genetics. 1995;139:1521-32 pubmed..Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth. ..
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