Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Germany
Topic: saccharomyces cerevisiae
Publications464 found, 100 shown here
- Wittke S, Lewke N, Muller S, Johnsson N. Probing the molecular environment of membrane proteins in vivo. Mol Biol Cell. 1999;10:2519-30 pubmed..The RUra3p reporter should further allow the selection of new binding partners of Sec63p and the selection of molecules or cellular conditions that interfere with the binding between Sec63p and one of its known partners. ..
- Oxelmark E, Marchini A, Malanchi I, Magherini F, Jaquet L, Hajibagheri M, et al. Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome. Mol Cell Biol. 2000;20:7784-97 pubmed..5, like Mmf1p, is a soluble protein of the matrix. Our study identifies a biological function for Mmf1p and furthermore indicates that this function is conserved between members of the p14.5 family. ..
- Walther K, Schüller H. Adr1 and Cat8 synergistically activate the glucose-regulated alcohol dehydrogenase gene ADH2 of the yeast Saccharomyces cerevisiae. Microbiology. 2001;147:2037-44 pubmed..In conclusion, these results show a derepression of ADH2 by synergistically acting regulators Adr1 (interacting with UAS1) and Cat8, binding to UAS2 (=CSRE(ADH2)). ..
- Fuchs K, Bommer G, Dumont E, Christoph B, Vidal M, Kremmer E, et al. Mutational analysis of the J recombination signal sequence binding protein (RBP-J)/Epstein-Barr virus nuclear antigen 2 (EBNA2) and RBP-J/Notch interaction. Eur J Biochem. 2001;268:4639-46 pubmed..Given the important role of RBP-J in B-cell immortalization, the EBNA2/RBP-J protein-protein interaction could be a candidate target for therapeutic intervention in EBV related diseases. ..
- Ragni E, Piberger H, Neupert C, García Cantalejo J, Popolo L, Arroyo J, et al. The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections. BMC Genomics. 2011;12:107 pubmed publisher..The microarray data produced in this analysis have been submitted to NCBI GEO database and GSE22649 record was assigned. ..
- Flemming D, Devos D, Schwarz J, Amlacher S, Lutzmann M, Hurt E. Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherins. J Struct Biol. 2012;177:99-105 pubmed publisher..Using bioinformatic methods, we have generated a pseudo-atomic structural model of Nup188 and its related Nup192, which further strengthens the view that the large ?-solenoid structural Nups are related to karyopherins. ..
- Frémont N, Riefler M, Stolz A, Schmülling T. The Arabidopsis TUMOR PRONE5 gene encodes an acetylornithine aminotransferase required for arginine biosynthesis and root meristem maintenance in blue light. Plant Physiol. 2013;161:1127-40 pubmed publisher..The inhibitory activity was specific for blue light, and the inhibiting light was perceived by the root. Thus, tup5-1 reveals a novel role of amino acids and blue light in regulating root meristem function...
- Wanzek K, Schwindt E, Capra J, Paeschke K. Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability. Nucleic Acids Res. 2017;45:7796-7806 pubmed publisher..Based on these data we propose a mechanistic model in which Mms1 binds to specific G-rich/G4 motif located on the lagging strand template for DNA replication and supports Pif1 function, DNA replication and genome integrity. ..
- Tschochner H. A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3' end. Proc Natl Acad Sci U S A. 1996;93:12914-9 pubmed..Shortened transcripts that remained associated within the ternary complexes were capable of resuming RNA chain elongation. Possible functions of the nuclease for transcript elongation or termination are discussed. ..
- Bauer A, Huber O, Kemler R. Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proc Natl Acad Sci U S A. 1998;95:14787-92 pubmed..We provide evidence for an in vivo multiprotein complex composed of Pontin52, beta-catenin, and lymphocyte enhancer factor-1/T-cell factor. Our results suggest involvement of Pontin52 in the nuclear function of beta-catenin. ..
- Taheri N, Kohler T, Braus G, Mösch H. Asymmetrically localized Bud8p and Bud9p proteins control yeast cell polarity and development. EMBO J. 2000;19:6686-96 pubmed..In response to nitrogen starvation, asymmetric localization of Bud9p is averted, favouring Bud8p-mediated cell division at the distal pole. ..
- Szyrach G, Ott M, Bonnefoy N, Neupert W, Herrmann J. Ribosome binding to the Oxa1 complex facilitates co-translational protein insertion in mitochondria. EMBO J. 2003;22:6448-57 pubmed..This suggests that co-translational membrane insertion of mitochondrial translation products is facilitated by a physical interaction of translation complexes with the membrane-bound translocase. ..
- Faou P, Tropschug M. Neurospora crassa CyPBP37: a cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B1. J Mol Biol. 2004;344:1147-57 pubmed publisher..In addition to its function in thiazole synthesis, CyPBP37 is a stress-inducible protein. N.crassa cyclophilin41 can chaperone the folding of CyPBP37, its own binding partner...
- Simader H, Suck D. Expression, purification, crystallization and preliminary phasing of the heteromerization domain of the tRNA-export and aminoacylation cofactor Arc1p from yeast. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:346-9 pubmed..8 angstroms resolution. Preliminary phasing reveals the presence of 20 monomers organized in five tetramers per asymmetric unit. ..
- Sahasrabudhe P, Rohrberg J, Biebl M, Rutz D, Buchner J. The Plasticity of the Hsp90 Co-chaperone System. Mol Cell. 2017;67:947-961.e5 pubmed publisher..Interestingly, some co-chaperones affect client conformation in vivo. Thus, co-chaperones adapt the Hsp90 cycle to the requirements of the client proteins, ensuring optimal activation. ..
- Voos W, Gambill B, Guiard B, Pfanner N, Craig E. Presequence and mature part of preproteins strongly influence the dependence of mitochondrial protein import on heat shock protein 70 in the matrix. J Cell Biol. 1993;123:119-26 pubmed
- Hobert O, Jallal B, Ullrich A. Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression. Mol Cell Biol. 1996;16:3066-73 pubmed..Interaction with ENX-1 suggests that Vav functions as an upstream element in the transcriptional regulation of homeobox genes, known to be important effectors in the hematopoietic system. ..
- Cormack R, Hahlbrock K, Somssich I. Isolation of putative plant transcriptional coactivators using a modified two-hybrid system incorporating a GFP reporter gene. Plant J. 1998;14:685-92 pubmed..The involvement of these proteins in general plant transcription as well as the advantages of using GFP as a reporter gene for detecting protein-protein interactions are discussed. ..
- Pries R, Bömeke K, Irniger S, Grundmann O, Braus G. Amino acid-dependent Gcn4p stability regulation occurs exclusively in the yeast nucleus. Eukaryot Cell. 2002;1:663-72 pubmed..Therefore, there is a strict spatial separation of protein synthesis and degradation of Gcn4p in yeast. Control of protein stabilization which antagonizes Gcn4p function is restricted to the nucleus. ..
- Englert M, Beier H. Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins. Nucleic Acids Res. 2005;33:388-99 pubmed..Hence, we conclude that their role in vivo might comprise yet unknown essential functions besides their involvement in pre-tRNA splicing. ..
- Mesecke N, Terziyska N, Kozany C, Baumann F, Neupert W, Hell K, et al. A disulfide relay system in the intermembrane space of mitochondria that mediates protein import. Cell. 2005;121:1059-69 pubmed..We suggest that this process reflects the evolutionary origin of the IMS from the periplasmic space of the prokaryotic ancestors of mitochondria. ..
- El Magraoui F, Bäumer B, Platta H, Baumann J, Girzalsky W, Erdmann R. The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner. FEBS J. 2012;279:2060-70 pubmed publisher..The RING domains proved to function as heteromeric pairs that display an Pex10p-dependent enhanced ligase activity in an ubiquitin conjugating enzyme-selective manner. ..
- Mueller S, Slany R. Structural analysis of the interaction of the tRNA modifying enzymes Tgt and QueA with a substrate tRNA. FEBS Lett. 1995;361:259-64 pubmed
- Beil B, Screaton G, Stamm S. Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. DNA Cell Biol. 1997;16:679-90 pubmed..The ratio of these two isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in regulation of htra2-beta activity. ..
- Adam A, Endres M, Sirrenberg C, Lottspeich F, Neupert W, Brunner M. Tim9, a new component of the TIM22.54 translocase in mitochondria. EMBO J. 1999;18:313-9 pubmed..10.12 complex and mediates partial translocation of mitochondrial carriers proteins across the outer membrane. The TIM9.10.12 complex assists further translocation into the inner membrane in association with TIM22.54. ..
- Heymann P, Ernst J, Winkelmann G. A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiae. Biometals. 2000;13:65-72 pubmed..The results are evidence that in S. cerevisiae the YOL158c Sce gene of the major facilitator super family, now designated ENB1, encodes a transporter protein (Enb1p), which specifically recognizes and transports enterobactin. ..
- Stein K, Schell Steven A, Erdmann R, Rottensteiner H. Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol. 2002;22:6056-69 pubmed..This complex accumulated in docking mutants but was absent in cells lacking Pex18p/Pex21p, indicating that Pex18p/Pex21p are required already before the docking event. ..
- Kruse C, Jaedicke A, Beaudouin J, Bohl F, Ferring D, Guttler T, et al. Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p. J Cell Biol. 2002;159:971-82 pubmed..Loss of She2p can be complemented by direct targeting of a heterologous lacZ mRNA to a complex of Myo4p and its associated adaptor She3p, suggesting that She2p's function in Myo4p targeting is to link an mRNA to the motor complex. ..
- Mokranjac D, Paschen S, Kozany C, Prokisch H, Hoppins S, Nargang F, et al. Tim50, a novel component of the TIM23 preprotein translocase of mitochondria. EMBO J. 2003;22:816-25 pubmed..We suggest that Tim50 plays a crucial role in the transfer of preproteins from the TOM complex to the TIM23 complex through the intermembrane space. ..
- Golbik R, Meshalkina L, Sandalova T, Tittmann K, Fiedler E, Neef H, et al. Effect of coenzyme modification on the structural and catalytic properties of wild-type transketolase and of the variant E418A from Saccharomyces cerevisiae. FEBS J. 2005;272:1326-42 pubmed
- Bagyan S, Mair T, Dulos E, Boissonade J, De Kepper P, Muller S. Glycolytic oscillations and waves in an open spatial reactor: Impact of feedback regulation of phosphofructokinase. Biophys Chem. 2005;116:67-76 pubmed..The generation of travelling NADH-waves is observed in the diffusive layer, when feeding the yeast extract with substrates. Break-up of circular-shaped waves is repeatedly observed, resulting in the formation of rotating NADH-spirals. ..
- Metz J, Wächter A, Schmidt B, Bujnicki J, Schwappach B. The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol. J Biol Chem. 2006;281:410-7 pubmed..Comparison of the deltaarr4 with the deltaarr4deltagef1 strain suggests that Arr4p antagonizes the function of Gef1p. ..
- Krappmann S, Jung N, Medic B, Busch S, Prade R, Braus G. The Aspergillus nidulans F-box protein GrrA links SCF activity to meiosis. Mol Microbiol. 2006;61:76-88 pubmed
- Albrecht R, Rehling P, Chacinska A, Brix J, Cadamuro S, Volkmer R, et al. The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. 2006;7:1233-8 pubmed..We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction. ..
- Heise B, van der Felden J, Kern S, Malcher M, Bruckner S, Mösch H. The TEA transcription factor Tec1 confers promoter-specific gene regulation by Ste12-dependent and -independent mechanisms. Eukaryot Cell. 2010;9:514-31 pubmed publisher..Our study suggests that Tec1 is able to regulate TCS-mediated gene expression by Ste12-dependent and Ste12-independent mechanisms that enable promoter-specific transcriptional control. ..
- Roggenbuck D, Reinhold D, Wex T, Goihl A, von Arnim U, Malfertheiner P, et al. Autoantibodies to GP2, the major zymogen granule membrane glycoprotein, are new markers in Crohn's disease. Clin Chim Acta. 2011;412:718-24 pubmed publisher..0307). Anti-GP2 IgG and IgA constitute novel CD specific autoantibodies, the quantification of which could improve the serological diagnosis of IBD. ..
- Schaaff Gerstenschläger I, Zimmermann F. Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase. Curr Genet. 1993;24:373-6 pubmed..Hybridization with low stringency showed the existence of a sequence with homology to transketolase, possibly a second gene. ..
- Stan T, Brix J, Schneider Mergener J, Pfanner N, Neupert W, Rapaport D. Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex. Mol Cell Biol. 2003;23:2239-50 pubmed
- Armbruster A, Bailer S, Koch M, Godovac Zimmermann J, Gruber G. Dimer formation of subunit G of the yeast V-ATPase. FEBS Lett. 2003;546:395-400 pubmed..G(38-144) is homogeneous, with a molecular mass of approximately 12+/-3 kDa, indicating a monomeric form in solution. ..
- Suzuki Y, Schwartz S, Mueller N, Schmitt M. Cysteine residues in a yeast viral A/B toxin crucially control host cell killing via pH-triggered disulfide rearrangements. Mol Biol Cell. 2017;28:1123-1131 pubmed publisher..In addition, we show that pH-triggered thiol rearrangements in K28 can cause the release of cytotoxic ? monomers, suggesting a toxin-intrinsic mechanism of disulfide bond reduction and ?/? heterodimer dissociation in the cytosol. ..
- Alconada A, Kubrich M, Moczko M, Hönlinger A, Pfanner N. The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins. Mol Cell Biol. 1995;15:6196-205 pubmed..These results suggest that Mom8b supports the cooperativity between receptors and the general insertion pore and facilitates the release of preproteins from import components and thereby promotes efficient transfer of preproteins. ..
- Boles E, de Jong Gubbels P, Pronk J. Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme. J Bacteriol. 1998;180:2875-82 pubmed..However, since null mutants could still grow anaerobically, this function is apparently not essential. ..
- Gottschalk A, Tang J, Puig O, Salgado J, Neubauer G, Colot H, et al. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA. 1998;4:374-93 pubmed..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP. ..
- Barz W, Walter P. Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins. Mol Biol Cell. 1999;10:1043-59 pubmed..Instead, we propose that Lag1p and Dgt1p facilitate efficient ER-to-Golgi transport of GPI-anchored proteins. ..
- Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
- Rose A, Meier I, Wienand U. The tomato I-box binding factor LeMYBI is a member of a novel class of myb-like proteins. Plant J. 1999;20:641-52 pubmed..LeMYBI acts as a transcriptional activator in yeast and plants, and binds to the I-box with a DNA-binding domain located in the carboxyterminal domain. ..
- Fiedler E, Thorell S, Sandalova T, Golbik R, König S, Schneider G. Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:591-5 pubmed..The intermediate is present with high occupancy in both active sites, arguing against previous proposals of half-of-the-sites reactivity in yeast TK. ..
- Muhlenhoff U, Gerber J, Richhardt N, Lill R. Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1p. EMBO J. 2003;22:4815-25 pubmed..We propose a model that dissects Fe/S cluster biogenesis into two major steps and assigns its central components to one of these two steps. ..
- Meier S, Neupert W, Herrmann J. Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria. J Biol Chem. 2005;280:7777-85 pubmed..On the basis of these observations we propose that charged residues in Tim17 are critical for the preprotein-induced gating of the TIM23 translocase. ..
- Shams Eldin H, Blaschke T, Anhlan D, Niehus S, Muller J, Azzouz N, et al. High-level expression of the Toxoplasma gondii STT3 gene is required for suppression of the yeast STT3 gene mutation. Mol Biochem Parasitol. 2005;143:6-11 pubmed
- Mokranjac D, Berg A, Adam A, Neupert W, Hell K. Association of the Tim14.Tim16 subcomplex with the TIM23 translocase is crucial for function of the mitochondrial protein import motor. J Biol Chem. 2007;282:18037-45 pubmed..Deletion of hydrophobic segments in both, Tim16 and Tim14, is lethal. We conclude that import into the matrix space of mitochondria requires association of the co-chaperones Tim16 and Tim14 with the TIM23 preprotein translocase. ..
- Milenkovic D, Gabriel K, Guiard B, Schulze Specking A, Pfanner N, Chacinska A. Biogenesis of the essential Tim9-Tim10 chaperone complex of mitochondria: site-specific recognition of cysteine residues by the intermembrane space receptor Mia40. J Biol Chem. 2007;282:22472-80 pubmed..Mia40 selectively recognizes cysteine-containing IMS proteins in a site-specific manner in organello and in vitro. Our results indicate that Mia40 acts as a trans receptor in the biogenesis of mitochondrial IMS proteins. ..
- Lin H, Fridy P, Ribeiro A, Choi J, Barma D, Vogel G, et al. Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases. J Biol Chem. 2009;284:1863-72 pubmed publisher..and Vogel, G. (1996) Biochem. J. 315, 715-725 ). Our study brings us closer to the goal of understanding the structure/function relationships that control specificity in the synthesis and biological actions of inositol pyrophosphates...
- Maier F, Zwicker S, Hückelhoven A, Meissner M, Funk J, Pfitzner A, et al. NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1) and some NPR1-related proteins are sensitive to salicylic acid. Mol Plant Pathol. 2011;12:73-91 pubmed publisher..The sensitivity of NPR1 proteins to SA, together with their differential interaction with diverse NIMIN proteins, seems a plausible molecular basis for the timely and coordinated activation of PR genes during SAR. ..
- Wysoczański P, Zweckstetter M. Retention and splicing complex (RES) - the importance of cooperativity. RNA Biol. 2016;13:128-33 pubmed publisher..Our work highlights the role of cooperativity in the spliceosome and poses new questions about the structure and assembly of the spliceosome. ..
- Chaudhary N, TÃ¸ndel K, Bhatnagar R, dos Santos V, PuchaÅ‚ka J. Characterizing the optimal flux space of genome-scale metabolic reconstructions through modified latin-hypercube sampling. Mol Biosyst. 2016;12:994-1005 pubmed publisher..Our method may be a useful device to validate the predictions made by FBA-based tools, by describing the optimal flux space associated with these predictions, thus to improve them. ..
- Schricker R, Magdolen V, Kaniak A, Wolf K, Bandlow W. The adenylate kinase family in yeast: identification of URA6 as a multicopy suppressor of deficiency in major AMP kinase. Gene. 1992;122:111-8 pubmed
- Destruelle M, Holzer H, Klionsky D. Isolation and characterization of a novel yeast gene, ATH1, that is required for vacuolar acid trehalase activity. Yeast. 1995;11:1015-25 pubmed..These results suggest that ATH1 may be the structural gene encoding vacuolar acid trehalase or that the gene product may be essential regulatory component involved in control of trehalase activity. ..
- Grandi P, Dang T, Pane N, Shevchenko A, Mann M, Forbes D, et al. Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol Biol Cell. 1997;8:2017-38 pubmed
- Kispal G, Csere P, Guiard B, Lill R. The ABC transporter Atm1p is required for mitochondrial iron homeostasis. FEBS Lett. 1997;418:346-50 pubmed..The increased mitochondrial iron content may be causative of the oxidative damage of heme-containing proteins in delta atm1 cells. Our data assign an important function to Atm1p in mitochondrial iron homeostasis...
- Enenkel C, Lehmann A, Kloetzel P. Subcellular distribution of proteasomes implicates a major location of protein degradation in the nuclear envelope-ER network in yeast. EMBO J. 1998;17:6144-54 pubmed..A major location of proteasomal peptide cleavage activity was visualized in the NE-ER network, indicating that proteasomal degradation takes place mainly in this subcellular compartment in yeast. ..
- Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
- Driller L, Wellinger R, Larrivee M, Kremmer E, Jaklin S, Feldmann H. A short C-terminal domain of Yku70p is essential for telomere maintenance. J Biol Chem. 2000;275:24921-7 pubmed..Therefore, the C-terminal domain in Yku70p defines at least one domain that is especially involved in telomere maintenance but not in NHEJ. ..
- Strasser K, Hurt E. Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. Nature. 2001;413:648-52 pubmed..Sub2p may then be displaced from Yra1p by the binding of Mex67p/Mtr2p, which participates in the export of mRNA through the nuclear pores. ..
- Buziol S, Bashir I, Baumeister A, Claassen W, Noisommit Rizzi N, Mailinger W, et al. New bioreactor-coupled rapid stopped-flow sampling technique for measurements of metabolite dynamics on a subsecond time scale. Biotechnol Bioeng. 2002;80:632-6 pubmed
- Milkereit P, Strauss D, Bassler J, Gadal O, Kühn H, Schütz S, et al. A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. J Biol Chem. 2003;278:4072-81 pubmed..Thus, Noc4p and Nop14p form a novel Noc complex with a specific role in nucleolar 40 S subunit formation and subsequent export to the cytoplasm. ..
- Windgassen M, Krebber H. Identification of Gbp2 as a novel poly(A)+ RNA-binding protein involved in the cytoplasmic delivery of messenger RNAs in yeast. EMBO Rep. 2003;4:278-83 pubmed..Together, our findings clearly show an involvement of Gbp2 in mRNA transport. In addition, as a non-essential protein, Gbp2 also has the interesting potential to be spatially or temporally regulated. ..
- Heyken W, Wagner C, Wittmann J, Albrecht A, Schüller H. Negative regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by a Candida albicans orthologue of OPI1. Yeast. 2003;20:1177-88 pubmed..albicans is mediated by a mechanism which does not involve recruitment of CaSin3 by CaOpi1. ..
- Sichting M, Mokranjac D, Azem A, Neupert W, Hell K. Maintenance of structure and function of mitochondrial Hsp70 chaperones requires the chaperone Hep1. EMBO J. 2005;24:1046-56 pubmed..This process is efficiently counteracted by Hep1. We conclude that Hep1 acts as a chaperone that is necessary and sufficient to prevent self-aggregation and to thereby maintain the function of the mitochondrial Hsp70 chaperones. ..
- Meinecke M, Wagner R, Kovermann P, Guiard B, Mick D, Hutu D, et al. Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science. 2006;312:1523-6 pubmed..Thus, the hydrophilic cis domain of Tim50 maintains the permeability barrier of mitochondria by closing the translocation pore in a presequence-regulated manner. ..
- Krick R, Tolstrup J, Appelles A, Henke S, Thumm M. The relevance of the phosphatidylinositolphosphat-binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy. FEBS Lett. 2006;580:4632-8 pubmed..GFP-Atg18-(FTTGT) and Atg21-(FTTGT)-GFP are released from vacuolar punctae to the cytosol. ..
- Platta H, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol. 2007;177:197-204 pubmed..Therefore, the energy requirement of the peroxisomal import pathway has to be extended by a second ATP-dependent step, namely receptor monoubiquitination. ..
- Rodicio R, Buchwald U, Schmitz H, Heinisch J. Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactis. Fungal Genet Biol. 2008;45:422-35 pubmed..Sensor-GFP fusions localize to the plasma membrane, with a cell cycle dependent distribution of KlWsc1-GFP. Both Wsc-type sensors concentrate in discrete spots within the plasma membrane. ..
- Würtz C, Schliebs W, Erdmann R, Rottensteiner H. Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein. FEBS J. 2008;275:2932-41 pubmed publisher..The data indicate that Woronin bodies emerge after the formation of a HEX1 core crystal within peroxisomes followed by Vps1p- and Dnm1p-mediated fission. ..
- Riedl J, Crevenna A, Kessenbrock K, Yu J, Neukirchen D, Bista M, et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods. 2008;5:605-7 pubmed publisher..Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. ..
- Reddehase S, Grumbt B, Neupert W, Hell K. The disulfide relay system of mitochondria is required for the biogenesis of mitochondrial Ccs1 and Sod1. J Mol Biol. 2009;385:331-8 pubmed publisher..In conclusion, the disulfide relay system is crucial for the import of Ccs1, thereby affecting the transport of Sod1, and it can control the distribution of Ccs1 and Sod1 between the IMS of mitochondria and the cytosol. ..
- Rogers D, McConnell E, Greig D. Molecular quantification of Saccharomyces cerevisiae ?-pheromone secretion. FEMS Yeast Res. 2012;12:668-74 pubmed publisher..This technique offers several improvements on current methods for measuring ?-pheromone production and will allow detailed investigation of the factors regulating pheromone production in yeast. ..
- Hüls D, Niessing D. Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:1251-4 pubmed publisher..02, c = 257.15?Å, ? = ? = 90, ? = 120°. The crystal that diffracted to 3?Å resolution belonged to space group P222, with unit-cell parameters a = 46.37, b = 74.64, c = 133.63?Å, ? = ? = ? = 90°. ..
- Psakhye I, Jentsch S. Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell. 2012;151:807-820 pubmed publisher..We propose that SUMOylation may thus often target a protein group rather than individual proteins, whereas localized modification enzymes and highly specific triggers ensure specificity. ..
- Missbach S, Weis B, Martin R, Simm S, Bohnsack M, Schleiff E. 40S ribosome biogenesis co-factors are essential for gametophyte and embryo development. PLoS ONE. 2013;8:e54084 pubmed publisher..All results support an involvement of the analyzed proteins in ribosome biogenesis but differences in rRNA processing, gametophyte and embryo development suggested an alternative regulation in plants. ..
- Moczko M, Schönfisch B, Voos W, Pfanner N, Rassow J. The mitochondrial ClpB homolog Hsp78 cooperates with matrix Hsp70 in maintenance of mitochondrial function. J Mol Biol. 1995;254:538-43 pubmed..We conclude that Hsp78 is required to keep mutant forms of mt-Hsp70 soluble and suggest a cooperation of Hsp78 and mt-Hsp70 in maintenance of essential mitochondrial functions. ..
- Cappellaro C, Baldermann C, Rachel R, Tanner W. Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin. EMBO J. 1994;13:4737-44 pubmed..cerevisiae agglutinins with that of the Dictyostelium contact site A glycoprotein (gp80), as well as with those of the various families of cell adhesion molecules of higher eucaryotes, have been made and are discussed. ..
- Kothe E. Tetrapolar fungal mating types: sexes by the thousands. FEMS Microbiol Rev. 1996;18:65-87 pubmed..Whereas heterobasidiomycetes display a bi-allelic structure for this locus with recognition between one receptor and the opposite pheromone, homobasidiomycetes contain multiple specificities for pheromone receptors and pheromones. ..
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