Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in France
Topic: saccharomyces cerevisiae
Publications441 found, 100 shown here
- Mouyna I, Kniemeyer O, Jank T, Loussert C, Mellado E, Aimanianda V, et al. Members of protein O-mannosyltransferase family in Aspergillus fumigatus differentially affect growth, morphogenesis and viability. Mol Microbiol. 2010;76:1205-21 pubmed publisher..These results indicate that the PMT family members play a major role in growth, morphogenesis and viability of A. fumigatus. ..
- Andrianaivomananjaona T, Moune Dimala M, Herga S, David V, Haraux F. How the N-terminal extremity of Saccharomyces cerevisiae IF1 interacts with ATP synthase: a kinetic approach. Biochim Biophys Acta. 2011;1807:197-204 pubmed publisher..Moreover, the data obtained using chimeric PsaE-IF1 suggest that before binding IF1 presents to the enzyme with its middle part facing a catalytic interface and its Nter extremity folded in the opposite direction. ..
- Pisani D, Rivoyre M, Ruel L, Bonino F, Bidet M, Dechesne C, et al. Mouse myodulin, a new potential angiogenic factor, functionally expressed in yeast. Biochem Biophys Res Commun. 2005;331:552-6 pubmed..The yield of purified myodulin should allow structure-function relationships studies for a better understanding of myodulin functional mechanisms. ..
- Desnoyers M, Giger Reverdin S, Bertin G, Duvaux Ponter C, Sauvant D. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. J Dairy Sci. 2009;92:1620-32 pubmed publisher..This effect could, however, be modulated by several different factors such as DMI, percentage of concentrate or NDF in the diet, or species...
- Chevtzoff C, Yoboue E, Galinier A, Casteilla L, Daignan Fornier B, Rigoulet M, et al. Reactive oxygen species-mediated regulation of mitochondrial biogenesis in the yeast Saccharomyces cerevisiae. J Biol Chem. 2010;285:1733-42 pubmed publisher..It is the first time that a redox sensitivity of the transcription factors involved in yeast mitochondrial biogenesis is shown. Such a process could be seen as a mitochondria quality control process. ..
- Joubert O, Nehmé R, Bidet M, Mus Veteau I. Heterologous expression of human membrane receptors in the yeast Saccharomyces cerevisiae. Methods Mol Biol. 2010;601:87-103 pubmed publisher..cerevisiae. Optimization of yeast culture and of MP preparations is detailed for two human receptors of the Hedgehog pathway: Patched and Smoothened. ..
- Sanchis D, Fleury C, Chomiki N, Goubern M, Huang Q, Neverova M, et al. BMCP1, a novel mitochondrial carrier with high expression in the central nervous system of humans and rodents, and respiration uncoupling activity in recombinant yeast. J Biol Chem. 1998;273:34611-5 pubmed
- Harismendy O, Gendrel C, Soularue P, Gidrol X, Sentenac A, Werner M, et al. Genome-wide location of yeast RNA polymerase III transcription machinery. EMBO J. 2003;22:4738-47 pubmed..This study fixes a reasonable upper bound to the number of class III genes in yeast and points to a global regulation at the level of Pol III and TFIIIB recruitment. ..
- Lafaye A, Junot C, Pereira Y, Lagniel G, Tabet J, Ezan E, et al. Combined proteome and metabolite-profiling analyses reveal surprising insights into yeast sulfur metabolism. J Biol Chem. 2005;280:24723-30 pubmed..Combined metabolome and proteome studies are necessary to draw a comprehensive and integrated view of cell metabolism. ..
- Defenouillère Q, Yao Y, Mouaikel J, Namane A, Galopier A, Decourty L, et al. Cdc48-associated complex bound to 60S particles is required for the clearance of aberrant translation products. Proc Natl Acad Sci U S A. 2013;110:5046-51 pubmed publisher..We propose that ubiquitination of the nascent peptide starts on the 80S and continues on the 60S, on which Cdc48 is recruited to escort the substrate for proteasomal degradation. ..
- Thuriaux P, Mariotte S, Buhler J, Sentenac A, Vu L, Lee B, et al. Gene RPA43 in Saccharomyces cerevisiae encodes an essential subunit of RNA polymerase I. J Biol Chem. 1995;270:24252-7 pubmed..RPA43 mapped next to RPA190, encoding the largest subunit of polymerase I. These genes are divergently transcribed and may thus share upstream regulatory elements ensuring their co-regulation. ..
- Riou C, Tourte Y, Lacroute F, Karst F. Isolation and characterization of a cDNA encoding Arabidopsis thaliana mevalonate kinase by genetic complementation in yeast. Gene. 1994;148:293-7 pubmed..Southern analysis suggests that probably only one locus exists in the A. thaliana genome. ..
- Loulergue C, Lebrun M, Briat J. Expression cloning in Fe2+ transport defective yeast of a novel maize MYC transcription factor. Gene. 1998;225:47-57 pubmed..The amount of 7E mRNA increased, in response to iron starvation, by 20 and 40% in roots and leaves, respectively. The relationship between iron metabolism and myc expression in animal cells is discussed. ..
- Marchal C, Haguenauer Tsapis R, Urban Grimal D. Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease. J Biol Chem. 2000;275:23608-14 pubmed..This may be due to CK1 having a second counteracting role in endocytosis as shown by the higher turnover of variant permeases with unphosphorylatable versions of the PEST sequence. ..
- Rohou H, Francisci S, Rinaldi T, Frontali L, Bolotin Fukuhara M. Reintroduction of a characterized Mit tRNA glycine mutation into yeast mitochondria provides a new tool for the study of human neurodegenerative diseases. Yeast. 2001;18:219-27 pubmed..The method will provide important applications for the use of yeast as a model system of human mitochondrial pathologies. ..
- Guglielmi B, Werner M. The yeast homolog of human PinX1 is involved in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition. J Biol Chem. 2002;277:35712-9 pubmed..Conversely, we found that Gno1p does not exhibit the in vivo telomerase inhibitor activity of PinX1. ..
- Dubacq C, Guerois R, Courbeyrette R, Kitagawa K, Mann C. Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast. Eukaryot Cell. 2002;1:568-82 pubmed..Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes. ..
- Blot G, Lopez Verges S, Treand C, Kubat N, Delcroix Genête D, Emiliani S, et al. Luman, a new partner of HIV-1 TMgp41, interferes with Tat-mediated transcription of the HIV-1 LTR. J Mol Biol. 2006;364:1034-47 pubmed..The interaction between the TMgp41 subunit of Env and Luman affects the stability of the full-length Luman protein, the precursor of the activated, nuclear form of Luman, which acts negatively on Tat-mediated HIV-1 transactivation. ..
- Auchère F, Santos R, Planamente S, Lesuisse E, Camadro J. Glutathione-dependent redox status of frataxin-deficient cells in a yeast model of Friedreich's ataxia. Hum Mol Genet. 2008;17:2790-802 pubmed publisher..In conclusion, our findings provide evidence for an impairment of glutathione homeostasis in a yeast model of Friedreich's ataxia and identify glutathione as a valuable indicator of the redox status of frataxin-deficient cells. ..
- Marullo P, Mansour C, Dufour M, Albertin W, Sicard D, Bely M, et al. Genetic improvement of thermo-tolerance in wine Saccharomyces cerevisiae strains by a backcross approach. FEMS Yeast Res. 2009;9:1148-60 pubmed publisher..Despite their common genetic background, these two strains showed slight physiological differences in response to environmental changes that enable identification of the key physiological parameters influencing stuck fermentation. ..
- Urban Grimal D, Pinson B, Chevallier J, Haguenauer Tsapis R. Replacement of Lys by Glu in a transmembrane segment strongly impairs the function of the uracil permease from Saccharomyces cerevisiae. Biochem J. 1995;308 ( Pt 3):847-51 pubmed..Lys-272 is fully conserved in the uracil permease family and is predicted to lie in the fourth transmembrane segment of the protein. It seems to be essential for both efficient uracil binding and translocation. ..
- Brun I, Sentenac A, Werner M. Dual role of the C34 subunit of RNA polymerase III in transcription initiation. EMBO J. 1997;16:5730-41 pubmed
- Pakdel F, Metivier R, Flouriot G, Valotaire Y. Two estrogen receptor (ER) isoforms with different estrogen dependencies are generated from the trout ER gene. Endocrinology. 2000;141:571-80 pubmed..These data suggest that the additional 45 residues at the N-terminal region of rtER(L) clearly modify the hormone-independent trans-activation function of the receptor. ..
- Verheggen C, Mouaikel J, Thiry M, Blanchard J, Tollervey D, Bordonne R, et al. Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain. EMBO J. 2001;20:5480-90 pubmed..We conclude that snoRNP assembly occurs either in the nucleoplasm, or during transit of snoRNAs through the NB, followed by routing of the complete snoRNP to functional sites of ribosome synthesis...
- Tougard P, Bizebard T, Ritco Vonsovici M, Minard P, Desmadril M. Structure of a circularly permuted phosphoglycerate kinase. Acta Crystallogr D Biol Crystallogr. 2002;58:2018-23 pubmed..The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein. ..
- Venard R, Brèthes D, Giraud M, Vaillier J, Velours J, Haraux F. Investigation of the role and mechanism of IF1 and STF1 proteins, twin inhibitory peptides which interact with the yeast mitochondrial ATP synthase. Biochemistry. 2003;42:7626-36 pubmed
- Rak M, Tetaud E, Duvezin Caubet S, Ezkurdia N, Bietenhader M, Rytka J, et al. A yeast model of the neurogenic ataxia retinitis pigmentosa (NARP) T8993G mutation in the mitochondrial ATP synthase-6 gene. J Biol Chem. 2007;282:34039-47 pubmed..The availability of a yeast NARP model could ease the search for rescuing mechanisms against this mitochondrial disease. ..
- Murakami H, Nicolas A. Locally, meiotic double-strand breaks targeted by Gal4BD-Spo11 occur at discrete sites with a sequence preference. Mol Cell Biol. 2009;29:3500-16 pubmed publisher..These results demonstrate that Spo11 itself has sequence preference and contributes to the choice of DSB positions. ..
- Giraud M, Paumard P, Sanchez C, Brèthes D, Velours J, Dautant A. Rotor architecture in the yeast and bovine F1-c-ring complexes of F-ATP synthase. J Struct Biol. 2012;177:490-7 pubmed publisher..yF(1)c(10)(II) provides a model of a more efficient generator. yF(1)c(10)(II) and bovine bF(1)c(8) structures share a common rotor architecture with the inertia center of the F(1)-stator close to the rotor axis. ..
- Garcia M, Apolinar Valiente R, Williams P, Esteve Zarzoso B, Arroyo T, Crespo J, et al. Polysaccharides and Oligosaccharides Produced on Malvar Wines Elaborated with Torulaspora delbrueckii CLI 918 and Saccharomyces cerevisiae CLI 889 Native Yeasts from D.O. "Vinos de Madrid". J Agric Food Chem. 2017;65:6656-6664 pubmed publisher..cerevisiae CLI 889. These findings help to provide important information about the polysaccharides and oligosaccharides released from the cell walls of Malvar grapes and the carbohydrates released from each yeast species. ..
- Bernard A, Mattei M, Pierres M, Marguet D. Structure of the mouse dipeptidyl peptidase IV (CD26) gene. Biochemistry. 1994;33:15204-14 pubmed..L., et al. (1992) Protein Eng. 5, 197-211], we report the possible evolutionary link between the DPP IV related family and alpha/beta hydrolases. ..
- Séron K, Blondel M, Haguenauer Tsapis R, Volland C. Uracil-induced down-regulation of the yeast uracil permease. J Bacteriol. 1999;181:1793-800 pubmed..These results suggest that uracil of exogenous or catabolic origin down-regulates the cognate permease to prevent buildup of excess intracellular uracil-derived nucleotides. ..
- Barbey R, Baudouin Cornu P, Lee T, Rouillon A, Zarzov P, Tyers M, et al. Inducible dissociation of SCF(Met30) ubiquitin ligase mediates a rapid transcriptional response to cadmium. EMBO J. 2005;24:521-32 pubmed..Post-translational control of SCF(Met30) assembly by a physiological stress to allow rapid induction of a protective gene expression program represents a novel mode of regulation in the ubiquitin system. ..
- Fronzes R, Weimann T, Vaillier J, Velours J, Brèthes D. The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits. Biochemistry. 2006;45:6715-23 pubmed
- Hobeika M, Brockmann C, Iglesias N, Gwizdek C, Neuhaus D, Stutz F, et al. Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67. Mol Biol Cell. 2007;18:2561-8 pubmed
- Clement T, Perez M, Mouret J, Sablayrolles J, Camarasa C. Use of a continuous multistage bioreactor to mimic winemaking fermentation. Int J Food Microbiol. 2011;150:42-9 pubmed publisher..It also allowed to uncouple the variations of environmental parameters and proved to be a promising tool to gain new insights into yeast metabolism during alcoholic fermentation. ..
- Loubbardi A, Marcireau C, Karst F, Guilloton M. Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase. J Bacteriol. 1995;177:1817-23 pubmed..Several putative Gcn4p binding sequences are present in the PDX3 promoter region, leading to the assumption that transcription of this gene is under the general control of nitrogen metabolism. ..
- Muñoz Centeno M, McBratney S, Monterrosa A, Byers B, Mann C, Winey M. Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Mol Biol Cell. 1999;10:2393-406 pubmed..Additionally, immunoelectron microscopy shows that GFP-Mps2p localizes to the SPB. Our analysis suggests that Mps2p is required as a component of the SPB for insertion of the nascent SPB into the nuclear envelope. ..
- Mireau H, Cosset A, Marechal Drouard L, Fox T, Small I, Dietrich A. Expression of Arabidopsis thaliana mitochondrial alanyl-tRNA synthetase is not sufficient to trigger mitochondrial import of tRNAAla in yeast. J Biol Chem. 2000;275:13291-6 pubmed..thaliana tRNA(Ala) nor of the endogenous cytosolic tRNA(Ala) isoacceptors. We conclude that at least one other factor besides the mitochondrial AlaRS precursor must be involved in mitochondrial import of tRNA(Ala) in plants. ..
- Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed..The association of these proteins is discussed in terms of NADH-channeling. ..
- Bresson A, Fuchs R. Lesion bypass in yeast cells: Pol eta participates in a multi-DNA polymerase process. EMBO J. 2002;21:3881-7 pubmed..In conclusion, this paper shows that, in yeast, translesion synthesis involves the combined action of several polymerases. ..
- Bailly E, Cabantous S, Sondaz D, Bernadac A, Simon M. Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci. 2003;116:4119-30 pubmed..Taken together, our data uncover a new role for Bud3 in cytokinesis that correlates with its capacity to target Clb2 at the neck, independently of its well established cell-type-specific function in bud site selection. ..
- Therizols P, Fairhead C, Cabal G, Genovesio A, Olivo Marin J, Dujon B, et al. Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region. J Cell Biol. 2006;172:189-99 pubmed..They also indicate that anchoring of telomeres is essential for efficient repair of DSBs occurring therein and is important for preserving genome integrity. ..
- Rak M, Tetaud E, Godard F, Sagot I, Salin B, Duvezin Caubet S, et al. Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology. J Biol Chem. 2007;282:10853-64 pubmed..However, the atp6 mutant exhibited unusual mitochondrial structure and distribution anomalies, presumably caused by a strong delay in inner membrane fusion. ..
- Kappei D, Londono Vallejo J. Telomere length inheritance and aging. Mech Ageing Dev. 2008;129:17-26 pubmed..Here, we review the most relevant experimental and descriptive data relating telomere length, as a quantitative trait, to aging and longevity. ..
- Diaz Ruiz R, Uribe Carvajal S, Devin A, Rigoulet M. Tumor cell energy metabolism and its common features with yeast metabolism. Biochim Biophys Acta. 2009;1796:252-65 pubmed publisher..cerevisiae as an early screening test in the research for novel anti-tumor compounds used for the inhibition of tumor cell metabolism. ..
- Novo M, Bigey F, Beyne E, Galeote V, Gavory F, Mallet S, et al. Eukaryote-to-eukaryote gene transfer events revealed by the genome sequence of the wine yeast Saccharomyces cerevisiae EC1118. Proc Natl Acad Sci U S A. 2009;106:16333-8 pubmed publisher..Our results suggest that these processes are favored by ecologic proximity and are involved in the molecular adaptation of wine yeasts to conditions of high sugar, low nitrogen, and high ethanol concentrations. ..
- Beckouët F, Mariotte Labarre S, Peyroche G, Nogi Y, Thuriaux P. Rpa43 and its partners in the yeast RNA polymerase I transcription complex. FEBS Lett. 2011;585:3355-9 pubmed publisher..Two-hybrid data and other genetic evidence suggest that Rpa43 directly bind Spt5, an RNAPI elongation factor also acting in RNAPII-dependent transcription, and may also interact with the nucleosomal chaperone Spt6. ..
- Kühl I, Fox T, Bonnefoy N. Schizosaccharomyces pombe homologs of the Saccharomyces cerevisiae mitochondrial proteins Cbp6 and Mss51 function at a post-translational step of respiratory complex biogenesis. Mitochondrion. 2012;12:381-90 pubmed publisher..pombe are not required for the translation of mitochondrial mRNAs, but fulfill post-translational functions, thus probably accounting for their conservation. ..
- Maguire S, Wang C, Holland L, Brunel F, Neuvéglise C, Nicaud J, et al. Zinc finger transcription factors displaced SREBP proteins as the major Sterol regulators during Saccharomycotina evolution. PLoS Genet. 2014;10:e1004076 pubmed publisher..Sre1 appears to have an ancestral role in the regulation of filamentation, which became decoupled from its role in sterol gene regulation by the arrival of Upc2 in the Saccharomycotina. ..
- ..Here, we use the modular organization of the evolutionary conserved Spt-Ada-Gcn5 acetyltransferase (SAGA) complex as a paradigm to illustrate how co-activators share and combine a relatively limited set of functional tools. ..
- Oulmouden A, Karst F. Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase. Curr Genet. 1991;19:9-14 pubmed..In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast. ..
- Lesuisse E, Horion B, Labbe P, Hilger F. The plasma membrane ferrireductase activity of Saccharomyces cerevisiae is partially controlled by cyclic AMP. Biochem J. 1991;280 ( Pt 2):545-8 pubmed..This drop was prevented in the double mutant cdc35 sra5 by adding cyclic AMP to the growth medium. We propose that ferrireductase activity is under the control of a cyclic AMP-dependent protein phosphorylation. ..
- Labbe Bois R. The ferrochelatase from Saccharomyces cerevisiae. Sequence, disruption, and expression of its structural gene HEM15. J Biol Chem. 1990;265:7278-83 pubmed..Disruption of the HEM15 gene led to non-viable cells in certain genetic background. Northern (RNA) analysis showed a slight (1.5-2-fold) repression of HEM15 expression by glucose. ..
- Manon S, Guerin M. Investigation of the effects of Zn2+ and Cu2+ on the K+ transport in yeast mitochondria. Evidences for the involvement of a Zn(2+)-binding protein in the K+/H+ exchange. Biochem Mol Biol Int. 1995;35:585-93 pubmed
- vom Baur E, Zechel C, Heery D, Heine M, Garnier J, Vivat V, et al. Differential ligand-dependent interactions between the AF-2 activating domain of nuclear receptors and the putative transcriptional intermediary factors mSUG1 and TIF1. EMBO J. 1996;15:110-24 pubmed..We discuss the possibility that mSUG1 and TIF1 may mediate the transcriptional activity of the AF-2 of nuclear receptors through different mechanisms. ..
- Arselin G, Vaillier J, Graves P, Velours J. ATP synthase of yeast mitochondria. Isolation of the subunit h and disruption of the ATP14 gene. J Biol Chem. 1996;271:20284-90 pubmed..The mutant mitochondria did not contain subunit h, and the mitochondrially encoded hydrophobic subunit 6 was not present. ..
- Marchal C, Haguenauer Tsapis R, Urban Grimal D. A PEST-like sequence mediates phosphorylation and efficient ubiquitination of yeast uracil permease. Mol Cell Biol. 1998;18:314-21 pubmed..A PEST-like sequence in which the serines were replaced by glutamic acids allowed efficient permease turnover, suggesting that the PEST serines are phosphoacceptors. ..
- Lemercier C, Verdel A, Galloo B, Curtet S, Brocard M, Khochbin S. mHDA1/HDAC5 histone deacetylase interacts with and represses MEF2A transcriptional activity. J Biol Chem. 2000;275:15594-9 pubmed
- Tréton B, Blanchin Roland S, Lambert M, Lepingle A, Gaillardin C. Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and paIH. Mol Gen Genet. 2000;263:505-13 pubmed..lipolytica and in S. cerevisiae. They affect invasiveness of haploid strains in S. cerevisiae only, and conjugation in Y. lipolytica only. These results highlight the conservation of the Pal pathway initially described in A. nidulans. ..
- Fourel G, Boscheron C, Revardel E, Lebrun E, Hu Y, Simmen K, et al. An activation-independent role of transcription factors in insulator function. EMBO Rep. 2001;2:124-32 pubmed..These findings predict that certain promoters behave as insulators and partition genomes in functionally independent domains. ..
- Hatin I, Fabret C, Namy O, Decatur W, Rousset J. Fine-tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome. Genetics. 2007;177:1527-37 pubmed..Among them, SSB1 and snR18, two factors close to the exit tunnel of the ribosome, directed the strongest antisuppression effects when overexpressed, showing that they may be involved in fine-tuning of the translation termination level. ..
- Muller H, Hennequin C, Gallaud J, Dujon B, Fairhead C. The asexual yeast Candida glabrata maintains distinct a and alpha haploid mating types. Eukaryot Cell. 2008;7:848-58 pubmed publisher..Nonetheless, cells maintain specificity through regulation of the alpha1 and alpha2 genes and, more surprisingly, through differential splicing of the a1 transcript. ..
- Foret J, de Courcy B, Gresh N, Piquemal J, Salmon L. Synthesis and evaluation of non-hydrolyzable D-mannose 6-phosphate surrogates reveal 6-deoxy-6-dicarboxymethyl-D-mannose as a new strong inhibitor of phosphomannose isomerases. Bioorg Med Chem. 2009;17:7100-7 pubmed publisher..The kinetic study reported here also suggests the dianionic character of the phosphate surrogate as a likely essential parameter for strong binding of the inhibitor to the enzyme active site. ..
- Tassan J, Le Guellec K, Kress M, Faure M, Camonis J, Jacquet M, et al. In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity. Mol Cell Biol. 1993;13:2815-21 pubmed..Our discovery of a structural and functional homolog in Xenopus cells implies that the function of SUP45 is not restricted to lower eukaryotes and that the SUP45 protein may perform a crucial cellular function in higher eukaryotes. ..
- Marsolier M, Prioleau M, Sentenac A. A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators. J Mol Biol. 1997;268:243-9 pubmed..This result indicates that this two-hybrid system can be used to assess the interactions between RNA polymerase II regulatory proteins and their partners. ..
- te Biesebeke R, Krab I, Parmeggiani A. The arginine finger loop of yeast and human GAP is a determinant for the specificity toward Ras GTPase. Biochemistry. 2001;40:7474-9 pubmed..Ras complexes emphasize the importance of the finger loop region not only for the catalytic activity but also as a structural determinant involved in the specificity of GAPs toward Ras proteins from different organisms. ..
- Breton A, Schaeffer J, Aigle M. The yeast Rvs161 and Rvs167 proteins are involved in secretory vesicles targeting the plasma membrane and in cell integrity. Yeast. 2001;18:1053-68 pubmed..These data support the idea that the Rvs proteins are involved in the late targeting of vesicles whose cargoes are required for cell wall construction. ..
- Boubekeur S, Camougrand N, Bunoust O, Rigoulet M, Guerin B. Participation of acetaldehyde dehydrogenases in ethanol and pyruvate metabolism of the yeast Saccharomyces cerevisiae. Eur J Biochem. 2001;268:5057-65 pubmed
- Delourme D, Lacroute F, Karst F. Cloning of an Arabidopsis thaliana cDNA coding for farnesyl diphosphate synthase by functional complementation in yeast. Plant Mol Biol. 1994;26:1867-73 pubmed..The predicted amino acid sequence presents about 50% identity with the yeast, rat and human FPP synthases. Southern blot analyses indicate that A. thaliana probably contains a single gene for farnesyl diphosphate synthase. ..
- Grandvaux N, Grizot S, Vignais P, Dagher M. The Ku70 autoantigen interacts with p40phox in B lymphocytes. J Cell Sci. 1999;112 ( Pt 4):503-13 pubmed
- Feuerbach F, Galy V, Trelles Sticken E, Fromont Racine M, Jacquier A, Gilson E, et al. Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast. Nat Cell Biol. 2002;4:214-21 pubmed..Our data support a model in which domains of enriched transcriptional regulators allow genes to determine transcriptional states by spatial positioning. ..
- Bugnicourt A, Froissard M, Sereti K, Ulrich H, Haguenauer Tsapis R, Galan J. Antagonistic roles of ESCRT and Vps class C/HOPS complexes in the recycling of yeast membrane proteins. Mol Biol Cell. 2004;15:4203-14 pubmed..Genetic analyses indicated that these phenotypes were due to the functioning of the Vps class C complex in trafficking both to and from the late endosomal compartment. ..
- Rébora K, Laloo B, Daignan Fornier B. Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule. Genetics. 2005;170:61-70 pubmed..Together our data clearly establish that AICAR is not just a metabolic intermediate but also acts as a true regulatory molecule. ..
- Collec E, El Nemer W, Gauthier E, Gane P, Lecomte M, Dhermy D, et al. Ubc9 interacts with Lu/BCAM adhesion glycoproteins and regulates their stability at the membrane of polarized MDCK cells. Biochem J. 2007;402:311-9 pubmed..Our results support a physiological role for the direct interaction between Lu gp and Ubc9 protein and reveal a role for this enzyme in regulating the stability of Lu gp at the cell membrane. ..
- Dziembowski A, Lorentzen E, Conti E, Seraphin B. A single subunit, Dis3, is essentially responsible for yeast exosome core activity. Nat Struct Mol Biol. 2007;14:15-22 pubmed..We observed that the yeast exosome ring mediates interactions with protein partners, providing an explanation for its essential function. ..
- Martineau C, Beckerich J, Kabani M. Flo11p-independent control of "mat" formation by hsp70 molecular chaperones and nucleotide exchange factors in yeast. Genetics. 2007;177:1679-89 pubmed..Importantly, these mutations did not compromise invasive growth or Flo11p expression, suggesting that Flo11p-independent pathways are necessary to form mats. ..
- Grigoras I, Lazard M, Plateau P, Blanquet S. Functional characterization of the Saccharomyces cerevisiae ABC-transporter Yor1p overexpressed in plasma membranes. Biochim Biophys Acta. 2008;1778:68-78 pubmed..Thus, competition of the drug with ATP binding is unlikely. Finally, we inspect possible hypotheses accounting for substrate inhibition, rather than stimulation, of ATP hydrolysis by the membrane preparation...
- Tavenet A, Suleau A, Dubreuil G, Ferrari R, Ducrot C, Michaut M, et al. Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription. Proc Natl Acad Sci U S A. 2009;106:14265-70 pubmed publisher..Sub1 was required for optimal Pol III gene transcription in exponentially growing cells. ..
- Bayot A, Gareil M, Rogowska Wrzesinska A, Roepstorff P, Friguet B, Bulteau A. Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1. J Biol Chem. 2010;285:11445-57 pubmed publisher..The results presented here supply evidence that Pim1-mediated proteolysis is required for elimination of oxidatively damaged proteins in mitochondria...
- Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont Racine M. Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export. RNA. 2010;16:1007-17 pubmed publisher..These data highlight the involvement of many factors acting together to export pre-60S particles. ..
- Celton M, Goelzer A, Camarasa C, Fromion V, Dequin S. A constraint-based model analysis of the metabolic consequences of increased NADPH oxidation in Saccharomyces cerevisiae. Metab Eng. 2012;14:366-79 pubmed publisher..Our findings emphasize the robustness of yeast to alterations in NADPH metabolism and highlight the role of the glycerol-DHA cycle as a redox valve, providing additional NADPH from NADH under conditions of very high demand. ..
- Martin G, Aguilée R, Ramsayer J, Kaltz O, Ronce O. The probability of evolutionary rescue: towards a quantitative comparison between theory and evolution experiments. Philos Trans R Soc Lond B Biol Sci. 2013;368:20120088 pubmed publisher..Finally, we use the results of the model to suggest further, more quantitative, tests of evolutionary rescue theory. ..
- Gavira C, Höfer R, Lesot A, Lambert F, Zucca J, Werck Reichhart D. Challenges and pitfalls of P450-dependent (+)-valencene bioconversion by Saccharomyces cerevisiae. Metab Eng. 2013;18:25-35 pubmed publisher..Based on these data, we propose new strategies for implementation of a viable P450-based bioconversion process. ..
- Brion C, Ambroset C, Sanchez I, Legras J, Blondin B. Differential adaptation to multi-stressed conditions of wine fermentation revealed by variations in yeast regulatory networks. BMC Genomics. 2013;14:681 pubmed publisher..This amplification has been detected among other wine yeasts. ..