Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Belgium
Topic: saccharomyces cerevisiae
Publications142 found, 100 shown here
- Amery L, Mannaerts G, Subramani S, Van Veldhoven P, Fransen M. Identification of a novel human peroxisomal 2,4-dienoyl-CoA reductase related protein using the M13 phage protein VI phage display technology. Comb Chem High Throughput Screen. 2001;4:545-52 pubmed..These results further demonstrate the suitability of the gVI-cDNA phage display technology for cDNA expression cloning using an antibody as a probe. ..
- El Alami M, Feller A, Piérard A, Dubois E. The proper folding of a long C-terminal segment of the yeast Lys14p regulator is required for activation of LYS genes in response to the metabolic effector. Mol Microbiol. 2002;43:1629-39 pubmed..Our results suggest that the proper folding of the two-thirds C-terminal portion of Lys14p is essential not only to activate transcription but also to modulate it according to alphaAASA concentration. ..
- Abdel Sater F, El Bakkoury M, Urrestarazu A, Vissers S, Andre B. Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Mol Cell Biol. 2004;24:9771-85 pubmed..Upon detection of external amino acids, Ssy1-Ptr3 likely allows-in a manner dependent on SCF(Grr1)-the Ssy5 endoprotease to gain access to and to cleave Stp1, this requiring prior phosphorylation of Stp1 by casein kinase I. ..
- Nikko E, Andre B. Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway. Traffic. 2007;8:566-81 pubmed..We propose that deubiquitination by Doa4 of cargo proteins and/or some components of the MVB sorting machinery is essential to correct sorting of cargoes into the MVB pathway. ..
- Swinnen E, Buttner S, Outeiro T, Galas M, Madeo F, Winderickx J, et al. Aggresome formation and segregation of inclusions influence toxicity of ?-synuclein and synphilin-1 in yeast. Biochem Soc Trans. 2011;39:1476-81 pubmed publisher..These models pointed towards a crucial role of the sirtuin Sir2 and the chaperonin complex TRiC (TCP-1 ring complex)/CCT (chaperonin containing TCP-1) in handling misfolded and aggregated proteins. ..
- Van Nieuwerburgh F, Thompson R, Ledesma J, Deforce D, Gaasterland T, Ordoukhanian P, et al. Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination. Nucleic Acids Res. 2012;40:e24 pubmed publisher..We also demonstrate how the resulting data significantly improves a de novo assembly of the S. cerevisiae genome. ..
- Kampfenkel K, Kushnir S, Babiychuk E, Inze D, Van Montagu M. Molecular characterization of a putative Arabidopsis thaliana copper transporter and its yeast homologue. J Biol Chem. 1995;270:28479-86 pubmed..Taken together, the data suggest that a plant metal transporter, which is most likely involved in the transport of copper, has been identified. ..
- van Dyck L, Pearce D, Sherman F. PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae. J Biol Chem. 1994;269:238-42 pubmed..PIM1 mRNA is constitutively expressed but is increased after thermal stress, suggesting that Pim1 may play a role in the heat shock response. ..
- Hoffman I, Demetter P, Peeters M, De Vos M, Mielants H, Veys E, et al. Anti-saccharomyces cerevisiae IgA antibodies are raised in ankylosing spondylitis and undifferentiated spondyloarthropathy. Ann Rheum Dis. 2003;62:455-9 pubmed..No correlation between the presence of subclinical bowel inflammation and ASCA IgA levels was noted. However, it remains to be evaluated whether patients with SpA with ASCA have an increased risk of developing CD. ..
- Abdel Sater F, Iraqui I, Urrestarazu A, Andre B. The external amino acid signaling pathway promotes activation of Stp1 and Uga35/Dal81 transcription factors for induction of the AGP1 gene in Saccharomyces cerevisiae. Genetics. 2004;166:1727-39 pubmed..Finally, the data show that the TOR pathway mediating global nitrogen control of transcription does not interfere with the external amino acid signaling pathway. ..
- Van Hoof C, Martens E, Longin S, Jordens J, Stevens I, Janssens V, et al. Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2. Biochem J. 2005;386:93-102 pubmed..Ypa1 as well as Ypa2 can reactivate all these inactive complexes, except Pph22-Yme. Ypa1 is the most potent activator of PP2A activity, suggesting that there is no direct correlation between activation potential and binding capacity. ..
- De Smet K, Reekmans R, Contreras R. Role of oxidative phosphorylation in histatin 5-induced cell death in Saccharomyces cerevisiae. Biotechnol Lett. 2004;26:1781-5 pubmed..cerevisiae. Incubation of CCCP-treated S. cerevisiae cells with histatin 5 still resulted in cell death. These results suggest that histatin 5-induced cell death in S. cerevisiae differs from that in C. albicans. ..
- Nikko E, Andre B. Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. Eukaryot Cell. 2007;6:1266-77 pubmed..Our data indicate that Bro1 interacts with several ESCRT components and contributes via its PRD to associating ubiquitinating and deubiquitinating enzymes with the MVB sorting machinery. ..
- Claessens A, Weyn C, Merregaert J. The cytoplasmic domain of chondrolectin interacts with the beta-subunit of Rab geranylgeranyl transferase. Cell Mol Biol Lett. 2008;13:250-9 pubmed..The interaction was confirmed with in vitro transcription/translation and co-immunoprecipitation (co-IP) experiments. ..
- Boeckstaens M, Andre B, Marini A. Distinct transport mechanisms in yeast ammonium transport/sensor proteins of the Mep/Amt/Rh family and impact on filamentation. J Biol Chem. 2008;283:21362-70 pubmed publisher..Our overall data suggest the existence of two functional groups of Mep/Amt-type proteins with different transport mechanisms and distinct impacts on cell physiology and signaling. ..
- Thevissen K, Yen W, Carmona Gutierrez D, Idkowiak Baldys J, Aerts A, Francois I, et al. Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;303:163-8 pubmed publisher..These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process. ..
- De Coninck B, Carron D, Tavormina P, Willem L, Craik D, Vos C, et al. Mining the genome of Arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance. J Exp Bot. 2013;64:5297-307 pubmed publisher..In conclusion, the identification and characterization of OSIP108 confirms the validity of our high-throughput approach, based on tiling array analysis in A. thaliana and functional screening in yeast, to identify bioactive peptides. ..
- Kempf C, Lengeler K, WENDLAND J. Differential stress response of Saccharomyces hybrids revealed by monitoring Hsp104 aggregation and disaggregation. Microbiol Res. 2017;200:53-63 pubmed publisher..The S. cerevisiae/S. paradoxus hybrid, combining the two most closely related strains, performed best under these conditions. ..
- Andre B, Scherens B. The yeast YBR235w gene encodes a homolog of the mammalian electroneutral Na(+)-(K+)-C1- cotransporter family. Biochem Biophys Res Commun. 1995;217:150-3 pubmed..We hypothesize that this yeast protein also mediates active uptake of C1- into the cell. ..
- Lecomte F, Szpirer J, Szpirer C. The S3a ribosomal protein gene is identical to the Fte-1 (v-fos transformation effector) gene and the TNF-alpha-induced TU-11 gene, and its transcript level is altered in transformed and tumor cells. Gene. 1997;186:271-7 pubmed..Like the Rps3a transcript level, the c-Fos transcript level is higher in transformed cells. Rps3a and Fos could thus be effectors of the transformed phenotype. ..
- Springael J, Andre B. Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1253-63 pubmed..We show here that mutant Gap1 permeases affected in these sequences still bind Ub. Furthermore, we provide evidence that only a small fraction of Gap1 is modified by Ub after addition of NH4+ to mutants defective in endocytosis. ..
- Babiychuk E, Cottrill P, Storozhenko S, Fuangthong M, Chen Y, O Farrell M, et al. Higher plants possess two structurally different poly(ADP-ribose) polymerases. Plant J. 1998;15:635-45 pubmed..The app promoter was transcriptionally up-regulated in cells pre-determined to die because of deficiency in a DNA ligase I. ..
- Douette P, Navet R, Bouillenne F, Brans A, Sluse Goffart C, Matagne A, et al. Secondary-structure characterization by far-UV CD of highly purified uncoupling protein 1 expressed in yeast. Biochem J. 2004;380:139-45 pubmed
- Maes D, Crabeel M, Van de Weerdt C, Martial J, Peeters E, Charlier D, et al. Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:1294-7 pubmed..The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 A, and a data set was collected to 2.76 A. ..
- Stuyven E, Verdonck F, van Hoek I, Daminet S, Duchateau L, Remon J, et al. Oral administration of beta-1,3/1,6-glucan to dogs temporally changes total and antigen-specific IgA and IgM. Clin Vaccine Immunol. 2010;17:281-5 pubmed publisher..The effects disappeared 1 week after the cessation of the supplementation. In conclusion, the results showed a temporary change in the isotype profile during glucan supplementation. ..
- Feller A, Georis I, Tate J, Cooper T, Dubois E. Alterations in the Ure2 ?Cap domain elicit different GATA factor responses to rapamycin treatment and nitrogen limitation. J Biol Chem. 2013;288:1841-55 pubmed publisher..This in turn is more consistent with the existence of distinct pathways for TorC1- and nitrogen limitation-dependent control than it is with these stimuli representing sequential steps in a single regulatory pathway. ..
- Thevissen K, Cammue B, Lemaire K, Winderickx J, Dickson R, Lester R, et al. A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii). Proc Natl Acad Sci U S A. 2000;97:9531-6 pubmed..Our data support a model in which membrane patches containing sphingolipids act as binding sites for DmAMP1 or, alternatively, are required to anchor membrane or cell wall-associated proteins, which themselves interact with DmAMP1. ..
- Belles Boix E, Babiychuk E, Montagu M, Inze D, Kushnir S. CEF, a sec24 homologue of Arabidopsis thaliana, enhances the survival of yeast under oxidative stress conditions. J Exp Bot. 2000;51:1761-2 pubmed..CEF is homologous to animal and yeast Sec24 proteins. These data suggest that CEF plays a protective role through protein transport during growth under pro-oxidant conditions. ..
- Amery L, Sano H, Mannaerts G, Snider J, Van Looy J, Fransen M, et al. Identification of PEX5p-related novel peroxisome-targeting signal 1 (PTS1)-binding proteins in mammals. Biochem J. 2001;357:635-46 pubmed..Based on its subcellular localization and binding properties, Pex5Rp may function as a regulator in an early step of the PTS1 protein import process. ..
- Vrancken G, Rimaux T, Weckx S, Leroy F, De Vuyst L. Influence of temperature and backslopping time on the microbiota of a type I propagated laboratory wheat sourdough fermentation. Appl Environ Microbiol. 2011;77:2716-26 pubmed publisher..The sourdough-typical species Lactobacillus sanfranciscensis was not found. Finally, a nonflour origin for this species was hypothesized. ..
- Wilms T, Swinnen E, Eskes E, Dolz Edo L, Uwineza A, Van Essche R, et al. The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability. PLoS Genet. 2017;13:e1006835 pubmed publisher..As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing. ..
- Van Mullem V, Landrieux E, Vandenhaute J, Thuriaux P. Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol. 2002;43:1105-13 pubmed..Thus, the N-terminal zinc domain of Rpa12p determines its anchoring to RNA polymerase I and is the only critical part of that subunit in vivo. ..
- Nikko E, Marini A, Andre B. Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway. J Biol Chem. 2003;278:50732-43 pubmed..This recycling seems to be accompanied by deubiquitination of the permease and unexpectedly requires a normal endosome-to-vacuole transport function. ..
- Vanlandschoot P, Van Houtte F, Ulrichts P, Tavernier J, Leroux Roels G. Immunostimulatory potential of hepatitis B nucleocapsid preparations: lipopolysaccharide contamination should not be overlooked. J Gen Virol. 2005;86:323-31 pubmed..This raises doubts on some of the underlying mechanisms that have been proposed to explain the unique immunogenicity of the HBV nucleocapsid...
- Kimpe M, Voordeckers K, Thevelein J, Van Zeebroeck G. Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2? system. Biochem Biophys Res Commun. 2012;419:89-94 pubmed publisher..Hence, the physiological importance of the close interactions between Pkh1 and Gcn2 or eIF2 could depend on other conditions and/or other targets of the Gcn2/eIF2 system. ..
- Iraqui I, Vissers S, Cartiaux M, Urrestarazu A. Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily. Mol Gen Genet. 1998;257:238-48 pubmed..An autonomously replicating sequence (ARS) element is located between the ARO8 gene and YGL201c which encodes a protein of the minichromosome maintenance family. ..
- Cherifi S, Robberecht J, Miendje Y. Saccharomyces cerevisiae fungemia in an elderly patient with Clostridium difficile colitis. Acta Clin Belg. 2004;59:223-4 pubmed..boulardii in association with vancomycin for Clostridium difficile colitis. We do not recommend administering this viable yeast particularly in debilited patient with active colitis. ..
- Reekmans R, De Smet K, Chen C, Van Hummelen P, Contreras R. Old yellow enzyme interferes with Bax-induced NADPH loss and lipid peroxidation in yeast. FEMS Yeast Res. 2005;5:711-25 pubmed..Our results clearly indicate that OYE3 interferes with Bax- and H(2)O(2)-induced lipid peroxidation and cell death in Saccharomyces cerevisiae. ..
- Faust K, Croes D, van Helden J. Metabolic pathfinding using RPAIR annotation. J Mol Biol. 2009;388:390-414 pubmed publisher..Our pathfinding approach is available as part of the Network Analysis Tools. ..
- Sharma S, Yang J, van Nues R, Watzinger P, Kötter P, Lafontaine D, et al. Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation. PLoS Genet. 2017;13:e1006804 pubmed publisher..Compared to yeast, the number of orphan box C/D snoRNAs in higher eukaryotes is much larger and we hypothesize that several of these may be involved in base-modifications. ..
- Balzi E, Choder M, Chen W, Varshavsky A, Goffeau A. Cloning and functional analysis of the arginyl-tRNA-protein transferase gene ATE1 of Saccharomyces cerevisiae. J Biol Chem. 1990;265:7464-71 pubmed..Null ate1 mutants are viable but lack the Arg-transferase activity and are unable to degrade those substrates of the N-end rule pathway that start with residues recognized by the Arg-transferase. ..
- Francois I, Bink A, Vandercappellen J, Ayscough K, Toulmay A, Schneiter R, et al. Membrane rafts are involved in intracellular miconazole accumulation in yeast cells. J Biol Chem. 2009;284:32680-5 pubmed publisher..We further show that this primary role of membrane rafts in miconazole action consists of mediating intracellular accumulation of miconazole in yeast cells. ..
- Vandenbosch D, De Canck E, Dhondt I, Rigole P, Nelis H, Coenye T. Genomewide screening for genes involved in biofilm formation and miconazole susceptibility in Saccharomyces cerevisiae. FEMS Yeast Res. 2013;13:720-30 pubmed publisher..Additionally, our data confirm previous findings on genes involved in biofilm formation and in general stress responses. Our data suggest the involvement of peroxisomes in biofilm formation and miconazole resistance in fungal biofilms. ..
- Babiychuk E, Kushnir S, Belles Boix E, Van Montagu M, Inze D. Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. J Biol Chem. 1995;270:26224-31 pubmed..The data suggested that proteins encoded by the isolated cDNAs play a distinct role in plant antioxidant defense and are possibly involved in NAD(P)/NAD(P)H homeostasis...
- Vranová E, Tähtiharju S, Sriprang R, Willekens H, Heino P, Palva E, et al. The AKT3 potassium channel protein interacts with the AtPP2CA protein phosphatase 2C. J Exp Bot. 2001;52:181-2 pubmed..A deletion analysis indicated that the catalytic domain of AtPP2CA was essential for the interaction with AKT3. Furthermore, the related PP2C phosphatase ABI1 did not interact with AKT3 in yeast. ..
- Lauwers E, Jacob C, Andre B. K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway. J Cell Biol. 2009;185:493-502 pubmed publisher..Our data reveal that K63-linked Ub chains act as a specific signal for MVB sorting, providing further insight into the Ub code of membrane protein trafficking. ..
- Stalmans S, Wynendaele E, Bracke N, Gevaert B, D Hondt M, Peremans K, et al. Chemical-functional diversity in cell-penetrating peptides. PLoS ONE. 2013;8:e71752 pubmed publisher..e. positive charge and amphipathicity, the shape, structure complexity and the 3D-pattern of constituting atoms influence the cellular uptake capacity of peptides. ..
- Decamps K, Joye I, De Vos D, Courtin C, Delcour J. Molecular Oxygen and Reactive Oxygen Species in Bread-making Processes: Scarce, but Nevertheless Important. Crit Rev Food Sci Nutr. 2016;56:722-36 pubmed publisher..In any case, dough turns anaerobic during the first minutes of fermentation...
- Marini A, Matassi G, Raynal V, Andre B, Cartron J, Cherif Zahar B. The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet. 2000;26:341-4 pubmed..Our results provide the first experimental evidence for a direct role of RhAG and RhGK in ammonium transport. These findings are of high interest, because no specific ammonium transport system has been characterized so far in human. ..
- Bogaerts S, Vanlandschoot A, van Hengel J, van Roy F. Nuclear translocation of alphaN-catenin by the novel zinc finger transcriptional repressor ZASC1. Exp Cell Res. 2005;311:1-13 pubmed..The interaction and nuclear cotranslocation of a neural alpha-catenin with a putative proto-oncogene product as reported here provides novel insights into the signaling functions of alpha-catenins. ..
- Aerts A, Francois I, Bammens L, Cammue B, Smets B, Winderickx J, et al. Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast. FEBS Lett. 2006;580:1903-7 pubmed..In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed. ..
- Giots F, Donaton M, Thevelein J. Inorganic phosphate is sensed by specific phosphate carriers and acts in concert with glucose as a nutrient signal for activation of the protein kinase A pathway in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2003;47:1163-81 pubmed
- Velasco I, Tenreiro S, Calderon I, Andre B. Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids. Eukaryot Cell. 2004;3:1492-503 pubmed..We propose that Aqr1 catalyzes transport of excess amino acids into vesicles, which then release them in the extracellular space by exocytosis. ..
- Chen W, Balzi E, Capieaux E, Choder M, Goffeau A. The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance. Yeast. 1991;7:287-99 pubmed
- Landrieu I, Vandenbol M, Hartlein M, Portetelle D. Mitochondrial asparaginyl-tRNA synthetase is encoded by the yeast nuclear gene YCR24c. Eur J Biochem. 1997;243:268-73 pubmed..The enzyme aminoacylated unfractionated Escherichia coli tRNA with asparagine. These results identified YCR24c as the structural gene for yeast mitochondrial asparaginyl-tRNA synthetase. ..
- Galland N, De Walque S, Voncken F, Verlinde C, Michels P. An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes. Mol Biochem Parasitol. 2010;171:45-9 pubmed publisher..In yeasts, as in most other organisms, TPI is indeed exclusively present in the cytosol. These results suggest that it may be possible to develop new trypanocidal drugs by targeting specifically the glycosome import mechanism of TPI...
- Viaene A, Volckaert G, Joniau M, De Baetselier A, Van Cauwelaert F. Efficient expression of bovine alpha-lactalbumin in Saccharomyces cerevisiae. Eur J Biochem. 1991;202:471-7 pubmed..The secreted bovine alpha-lactalbumin is enzymatically active and identical to the whey protein, as confirmed by SDS/PAGE, IEF, ultraviolet and CD spectral analysis, and amino-terminal sequence determination. ..
- Storozhenko S, Inze D, Van Montagu M, Kushnir S. Arabidopsis coactivator ALY-like proteins, DIP1 and DIP2, interact physically with the DNA-binding domain of the Zn-finger poly(ADP-ribose) polymerase. J Exp Bot. 2001;52:1375-80 pubmed..Their homology to the transcriptional coactivator ALY suggests that plant PARP may play a role in the regulation of transcription. ..
- Thevissen K, Idkowiak Baldys J, Im Y, Takemoto J, Francois I, Ferket K, et al. SKN1, a novel plant defensin-sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis. FEBS Lett. 2005;579:1973-7 pubmed..These results show that SKN1, together with IPT1, is involved in sphingolipid biosynthesis in S. cerevisiae. ..
- Kellner H, Luis P, Portetelle D, Vandenbol M. Screening of a soil metatranscriptomic library by functional complementation of Saccharomyces cerevisiae mutants. Microbiol Res. 2011;166:360-8 pubmed publisher..The sequenced insert showed strong similarity to a basidiomycete acid phosphatase (60% identity to Postia placenta). ..
- Qiu H, Dubois E, Broen P, Messenguy F. Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Gen Genet. 1990;222:192-200 pubmed..Gene disruption of the ARGRIII gene impairs the growth of the mutant on rich medium, showing that ARGRIII has a pleiotropic role in the cell. ..
- Legrain C, Villeret V, Roovers M, Gigot D, Dideberg O, Pi rard A, et al. Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus. Eur J Biochem. 1997;247:1046-55 pubmed..Recombinant P. furiosus OTCase synthesised by Escherichia coli proved less stable than the native enzyme. In Saccharomyces cerevisiae, however, an enzyme apparently identical to the native one could be obtained...
- De Valck D, Jin D, Heyninck K, Van de Craen M, Contreras R, Fiers W, et al. The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases. Oncogene. 1999;18:4182-90 pubmed..caspase-3, caspase-6 and caspase-7. Thus TXBP151 appears to be a novel A20-binding protein which might mediate the anti-apoptotic activity of A20, and which can be processed by specific caspases. ..
- Bizimungu C, De Neve N, Burny A, Bach S, Bontemps F, Portetelle D, et al. Expression in a RabGAP yeast mutant of two human homologues, one of which is an oncogene. Biochem Biophys Res Commun. 2003;310:498-504 pubmed..As two highly conserved amino acids, including the catalytic arginine, are mutated in the oncTre210p TBC domain, we restored these two amino acids and expressed the modified Tre2 cDNA in the yeast mutant. ..
- Saerens S, Delvaux F, Verstrepen K, Thevelein J. Production and biological function of volatile esters in Saccharomyces cerevisiae. Microb Biotechnol. 2010;3:165-77 pubmed publisher..This paper reviews the current hypotheses of ester synthesis in yeast in relation to the complex regulation of the alcohol acetyl transferases and the different factors that allow ester formation to be controlled during fermentation. ..
- Ramos F, Dubois E, Pierard A. Control of enzyme synthesis in the lysine biosynthetic pathway of Saccharomyces cerevisiae. Evidence for a regulatory role of gene LYS14. Eur J Biochem. 1988;171:171-6 pubmed..The leaky auxotrophic behaviour of lys14 mutants is explained by the low basal level of expression of LYS9, the structural gene of this enzyme, in the absence of induction by 2-aminoadipate semialdehyde. ..
- Van Hoof C, Cayla X, Bosch M, Merlevede W, Goris J. The phosphotyrosyl phosphatase activator of protein phosphatase 2A. A novel purification method, immunological and enzymic characterization. Eur J Biochem. 1994;226:899-907 pubmed..Mg, a necessary cofactor in the activation process. Interaction of PTPA with PP2A in a 1:1 ratio induces a low (kcat = 3 min-1) ATPase activity that is inhibited by okadaic acid, ADP and non-hydrolysable ATP analogues. ..
- Hohmann S, Van Dijck P, Luyten K, Thevelein J. The byp1-3 allele of the Saccharomyces cerevisiae GGS1/TPS1 gene and its multi-copy suppressor tRNA(GLN) (CAG): Ggs1/Tps1 protein levels restraining growth on fermentable sugars and trehalose accumulation. Curr Genet. 1994;26:295-301 pubmed..Sequence analysis of the cloned tRNA(GLN) (CAG) gene showed that it is located 700 bp upstream of URA10. However, we found considerable differences to the reported sequence of URA10, in particular in the non-coding region. ..
- Andre B, Hein C, Grenson M, Jauniaux J. Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. Mol Gen Genet. 1993;237:17-25 pubmed..In the absence of the pleiotropic UGA43/DAL80 repressor, UGA4 is constitutively expressed at high level. ..
- de Winde J, Crauwels M, Hohmann S, Thevelein J, Winderickx J. Differential requirement of the yeast sugar kinases for sugar sensing in establishing the catabolite-repressed state. Eur J Biochem. 1996;241:633-43 pubmed..These results indicate that sugar sensing and establishment of catabolite repression are controlled by an interregulatory network, involving all three yeast sugar kinases and the Ras-cAMP pathway. ..
- Iraqui I, Vissers S, Bernard F, De Craene J, Boles E, Urrestarazu A, et al. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol Cell Biol. 1999;19:989-1001 pubmed..The essential role of Grr1p in this amino acid signaling pathway lends further support to the hypothesis that this protein participates in integrating nutrient availability with the cell cycle. ..
- De Craene J, Soetens O, Andre B. The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease. J Biol Chem. 2001;276:43939-48 pubmed..Npr1 belongs to a subgroup of protein kinases, some of which are reported to exert a positive control on the activity of other permeases. We propose that these kinases also function as regulators of permease trafficking. ..
- El Alami M, Messenguy F, Scherens B, Dubois E. Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol. 2003;49:457-68 pubmed..Our results indicate that Arg82p is a bifunctional protein whose inositol kinase activity plays a role in multiple signalling cascades, and whose acidic domain protects two MADS-box proteins against degradation. ..
- Massant J, Wouters J, Glansdorff N. Refined structure of Pyrococcus furiosus ornithine carbamoyltransferase at 1.87 A. Acta Crystallogr D Biol Crystallogr. 2003;59:2140-9 pubmed..However, the interfaces between catalytic trimers in the dodecameric oligomer are mainly hydrophobic and also involve aromatic-aromatic and cation-pi interactions...
- Decottignies A, Evain A, Ghislain M. Binding of Cdc48p to a ubiquitin-related UBX domain from novel yeast proteins involved in intracellular proteolysis and sporulation. Yeast. 2004;21:127-39 pubmed..Together, these results indicate that the Cuil-3 proteins form complexes that are components of the ubiquitin-proteasome system. ..
- de Thozée C, Cronin S, Goj A, Golin J, Ghislain M. Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding fold. Mol Microbiol. 2007;63:811-25 pubmed..These results suggest that Pdr5p requires a properly folded nucleotide-binding domain for trafficking to the plasma membrane...
- Boeckstaens M, Andre B, Marini A. The yeast ammonium transport protein Mep2 and its positive regulator, the Npr1 kinase, play an important role in normal and pseudohyphal growth on various nitrogen media through retrieval of excreted ammonium. Mol Microbiol. 2007;64:534-46 pubmed..Finally, we show that Mep2 participates, through ammonium leakage compensation, in pseudohyphal growth induced by amino acid starvation. This argues further in favour of tight coupling of Mep2 transport and sensor functions. ..
- Popova Y, Thayumanavan P, Lonati E, Agrochão M, Thevelein J. Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor. Proc Natl Acad Sci U S A. 2010;107:2890-5 pubmed publisher..Our results provide to the best of our knowledge the first insight into the molecular mechanism of a phosphate transceptor. ..
- Roebroek A, Creemers J, Pauli I, Kurzik Dumke U, Rentrop M, Gateff E, et al. Cloning and functional expression of Dfurin2, a subtilisin-like proprotein processing enzyme of Drosophila melanogaster with multiple repeats of a cysteine motif. J Biol Chem. 1992;267:17208-15 pubmed..Finally, Dfur2 was mapped to region 14C of the X chromosome of D. melanogaster. ..
- Heimberg H, Boyen A, Crabeel M, Glansdorff N. Escherichia coli and Saccharomyces cerevisiae acetylornithine aminotransferase: evolutionary relationship with ornithine aminotransferase. Gene. 1990;90:69-78 pubmed..e., ornithine)-specific, S. cerevisiae ACOAT transaminates ornithine about as efficiently as E. coli does. The evolutionary relationship between ACOATs and OATs is discussed in terms of substrate ambiguity. ..
- Hein C, Springael J, Volland C, Haguenauer Tsapis R, Andre B. NPl1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase. Mol Microbiol. 1995;18:77-87 pubmed..Our results show that the Npi1/Rsp5 ubiquitin-protein ligase participates in induced degradation of at least two permeases, Gap1p and Fur4p, and probably also other proteins. ..
- Vanhooren J, Fransen M, de Béthune B, Baumgart E, Baes M, Torrekens S, et al. Rat pristanoyl-CoA oxidase. cDNA cloning and recognition of its C-terminal (SQL) by the peroxisomal-targeting signal 1 receptor. Eur J Biochem. 1996;239:302-9 pubmed..No mRNA could be detected by northern analysis of human tissues, suggesting that the human pristanoyl-CoA oxidase gene, if present, is only poorly or not transcribed. ..
- Crabeel M, Soetens O, De Rijcke M, Pratiwi R, Pankiewicz R. The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis. J Biol Chem. 1996;271:25011-8 pubmed..Arg11p thus appears to fulfill a partially redundant function requiring its 27 carboxyl-terminal amino acids. As a working hypothesis, we propose that Arg11p participates in the export of matrix-made ornithine into the cytosol. ..
- Matthijs G, Schollen E, Pirard M, Budarf M, Van Schaftingen E, Cassiman J. PMM (PMM1), the human homologue of SEC53 or yeast phosphomannomutase, is localized on chromosome 22q13. Genomics. 1997;40:41-7 pubmed..Since the CDG1 locus maps else where in the genome (16p13), mutations in the phosphomannomutase gene encoded by chromosome 22 are not a major cause of CDG1. ..
- Nelissen B, De Wachter R, Goffeau A. Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cerevisiae. FEMS Microbiol Rev. 1997;21:113-34 pubmed..The latter resulted in the identification of 21 subclusters with presumably tightly related permease function. No functional clues were predicted for a total of 41 clustered or unclustered proteins. ..
- van den Hazel H, Pichler H, do Valle Matta M, Leitner E, Goffeau A, Daum G. PDR16 and PDR17, two homologous genes of Saccharomyces cerevisiae, affect lipid biosynthesis and resistance to multiple drugs. J Biol Chem. 1999;274:1934-41 pubmed..Collectively, our results indicate that PDR16 and PDR17 control levels of various lipids in various compartments of the cell and thereby provide a mechanism for multidrug resistance unrecognized so far. ..