Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Austria
Topic: saccharomyces cerevisiae
Publications106 found, 100 shown here
- Wagner W, Bielli P, Wacha S, Ragnini Wilson A. Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. EMBO J. 2002;21:6397-408 pubmed..Further more, myosin light chains, via their ability to be transported by secretory vesicles and to interact with class V myosin IQ motifs, can regulate vesicle polarization processes at a specific location and stage of the cell cycle. ..
- Ott R, Athenstaedt K, Hrastnik C, Leitner E, Bergler H, Daum G. Flux of sterol intermediates in a yeast strain deleted of the lanosterol C-14 demethylase Erg11p. Biochim Biophys Acta. 2005;1735:111-8 pubmed
- Griffioen G, Anghileri P, Imre E, Baroni M, Ruis H. Nutritional control of nucleocytoplasmic localization of cAMP-dependent protein kinase catalytic and regulatory subunits in Saccharomyces cerevisiae. J Biol Chem. 2000;275:1449-56 pubmed
- Schneiter R, Guerra C, Lampl M, Tatzer V, Zellnig G, Klein H, et al. A novel cold-sensitive allele of the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme A carboxylase, affects the morphology of the yeast vacuole through acylation of Vac8p. Mol Cell Biol. 2000;20:2984-95 pubmed..The function of Vac8p in protein targeting, on the other hand, was not affected under these conditions. These observations link fatty acid synthesis and availability to direct morphological alterations of an organellar membrane. ..
- Gurvitz A, Hiltunen J, Erdmann R, Hamilton B, Hartig A, Ruis H, et al. Saccharomyces cerevisiae Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11. J Biol Chem. 2001;276:31825-30 pubmed..We conclude that overlapping ORE and UAS1 elements in conjunction with their binding factors Pip2p-Oaf1p and Adr1p coordinate the carbon flux through beta-oxidation with peroxisome proliferation. ..
- Felder T, Bogengruber E, Tenreiro S, Ellinger A, Sá Correia I, Briza P. Dtrlp, a multidrug resistance transporter of the major facilitator superfamily, plays an essential role in spore wall maturation in Saccharomyces cerevisiae. Eukaryot Cell. 2002;1:799-810 pubmed..Dtrlp is the first multidrug resistance protein of the major facilitator superfamily with an assigned physiological role in the yeast cell. ..
- Maier R, Brandner C, Hintner H, Bauer J, Onder K. Construction of a reading frame-independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening. Biotechniques. 2008;45:235-44 pubmed publisher..This new system could enable simple and fast large-scale PPI studies and the construction of high-quality screening libraries. ..
- Rosenberger S, Connerth M, Zellnig G, Daum G. Phosphatidylethanolamine synthesized by three different pathways is supplied to peroxisomes of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2009;1791:379-87 pubmed publisher..The fact that peroxisomes were always found in close vicinity to mitochondria, ER and lipid particles supported the view that membrane contact may play a role in lipid traffic between these organelles. ..
- Gaigg B, Simbeni R, Hrastnik C, Paltauf F, Daum G. Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involvement in synthesis and import of phospholipids into mitochondria. Biochim Biophys Acta. 1995;1234:214-20 pubmed..Reassociation of MAMs with purified mitochondria led to reconstitution of the import of phosphatidylserine into mitochondria. Organelle contact is suggested as a possible mechanism of this process. ..
- Karl T, Onder K, Kodzius R, Pichova A, Wimmer H, Th r A, et al. GRC5 and NMD3 function in translational control of gene expression and interact genetically. Curr Genet. 1999;34:419-29 pubmed..NMD3 was previously identified as a component of the nonsense-mediated mRNA decay pathway. The point mutations within NMD3 reported here may define a domain important for the functional interaction of Grc5p and Nmd3p. ..
- Kohlwein S, Eder S, Oh C, Martin C, Gable K, Bacikova D, et al. Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:109-25 pubmed..Tsc13p localizes to the endoplasmic reticulum and is highly enriched in a novel structure marking nuclear-vacuolar junctions. ..
- Toth A, Ciosk R, Uhlmann F, Galova M, Schleiffer A, Nasmyth K. Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev. 1999;13:320-33 pubmed..The sixth protein, Eco1p(Ctf7p), is not a Cohesin subunit. It is necessary for the establishment of cohesion during DNA replication but not for its maintenance during G2 and M phases. ..
- Grillari J, LÃ¶scher M, Denegri M, Lee K, Fortschegger K, Eisenhaber F, et al. Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4. J Biol Chem. 2009;284:29193-204 pubmed publisher..Thus we suggest that Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites. ..
- Buschhorn B, Petzold G, Galova M, Dube P, Kraft C, Herzog F, et al. Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol. 2011;18:6-13 pubmed publisher..Our results suggest that substrates are recruited to the APC/C by binding to a bipartite substrate receptor composed of a coactivator protein and Doc1. ..
- Hasslacher M, Ivessa A, Paltauf F, Kohlwein S. Acetyl-CoA carboxylase from yeast is an essential enzyme and is regulated by factors that control phospholipid metabolism. J Biol Chem. 1993;268:10946-52 pubmed..The 5'-untranslated region of the ACC1 gene contains a sequence reminiscent of an inositol/choline-responsive element identified in genes encoding phospholipid biosynthetic enzymes. ..
- VALASEK L, Hasek J, Trachsel H, Imre E, Ruis H. The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3. J Biol Chem. 1999;274:27567-72 pubmed..In a computer search, a significant homology to the human p35 subunit of the eIF3 complex was found. We assume that the yeast Hcr1 protein participates in translation initiation likely as a protein associated with the eIF3 complex. ..
- Müllner H, Zweytick D, Leber R, Turnowsky F, Daum G. Targeting of proteins involved in sterol biosynthesis to lipid particles of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2004;1663:9-13 pubmed..This result strongly supports the view that ER and lipid particles are related organelles. ..
- Arumugam P, Nishino T, Haering C, Gruber S, Nasmyth K. Cohesin's ATPase activity is stimulated by the C-terminal Winged-Helix domain of its kleisin subunit. Curr Biol. 2006;16:1998-2008 pubmed..In contrast, we do not detect any effect of Scc1's N-terminal domain on Smc1/Smc3 ATPase activity. Our studies reveal that Scc1 not only connects the Smc1 and Smc3 ATPase heads but also regulates their ATPase activity. ..
- Panizza S, Tanaka T, Hochwagen A, Eisenhaber F, Nasmyth K. Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol. 2000;10:1557-64 pubmed
- Pammer M, Briza P, Ellinger A, Schuster T, Stucka R, Feldmann H, et al. DIT101 (CSD2, CAL1), a cell cycle-regulated yeast gene required for synthesis of chitin in cell walls and chitosan in spore walls. Yeast. 1992;8:1089-99 pubmed..As most of the chitin synthesis of vegetative cells occurs at this stage of the cell division cycle, chitin synthesis mediated by DIT101 could be primarily regulated at the level of transcription in vegetatively growing cells. ..
- Zachariae W, Shevchenko A, Andrews P, Ciosk R, Galova M, Stark M, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science. 1998;279:1216-9 pubmed..Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication. ..
- Klein F, Mahr P, Galova M, Buonomo S, Michaelis C, Nairz K, et al. A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell. 1999;98:91-103 pubmed..The eukaryotic cell's cohesion apparatus is required both for the repair of recombinogenic lesions and for chromosome segregation and therefore appears to lie at the heart of the meiotic process. ..
- Steinboeck F, Krupanska L, Bogusch A, Kaufmann A, Heidenreich E. Novel regulatory properties of Saccharomyces cerevisiae Arp4. J Biochem. 2006;139:741-51 pubmed..The same effect was observed upon treatment with rapamycin, indicating an unexpected relationship of Arp4 to TOR-mediated cell cycle arrest. ..
- Ragnini A, Teply R, Waldherr M, Voskova A, Schweyen R. The yeast protein Mrs6p, a homologue of the rabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. Curr Genet. 1994;26:308-14 pubmed..We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions. ..
- Prein B, Natter K, Kohlwein S. A novel strategy for constructing N-terminal chromosomal fusions to green fluorescent protein in the yeast Saccharomyces cerevisiae. FEBS Lett. 2000;485:29-34 pubmed..In agreement with previous reports, GFP-Faa2p was found to localize to peroxisomes. ..
- Palecek J, Hasek J, Ruis H. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun. 2001;282:1244-50 pubmed..Considering the essential role of Rpg1p as the large subunit of the eIF3 core complex and the association of Sla2p with the actin cytoskeleton, a putative role of the Rpg1p-Sla2p interaction in localized translation is discussed. ..
- Laschober G, Ruli D, Hofer E, Muck C, Carmona Gutierrez D, Ring J, et al. Identification of evolutionarily conserved genetic regulators of cellular aging. Aging Cell. 2010;9:1084-97 pubmed publisher..In conclusion, an unbiased screen across species uncovered several so far unrecognized molecular pathways for cellular aging that are conserved in evolution. ..
- Kohlwein S, Kuchler K, Sperka Gottlieb C, Henry S, Paltauf F. Identification of mitochondrial and microsomal phosphatidylserine synthase in Saccharomyces cerevisiae as the gene product of the CHO1 structural gene. J Bacteriol. 1988;170:3778-81 pubmed..In the absence of protease inhibitors during the preparation of membranes, the enzyme underwent degradation to an enzymatically active protein of 23,000 apparent molecular weight. ..
- Mahe Y, Lemoine Y, Kuchler K. The ATP binding cassette transporters Pdr5 and Snq2 of Saccharomyces cerevisiae can mediate transport of steroids in vivo. J Biol Chem. 1996;271:25167-72 pubmed
- Zachariae W, Schwab M, Nasmyth K, Seufert W. Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex. Science. 1998;282:1721-4 pubmed..The mutual inhibition between APC and CDKs explains how cells suppress mitotic CDK activity during G1 and then establish a period with elevated kinase activity from S phase until anaphase. ..
- Reiser V, Ruis H, Ammerer G. Kinase activity-dependent nuclear export opposes stress-induced nuclear accumulation and retention of Hog1 mitogen-activated protein kinase in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1147-61 pubmed..Thus, at least three different mechanisms contribute to the intracellular distribution pattern of Hog1: phosphorylation-dependent nuclear accumulation, retention by nuclear targets, and a kinase-induced export. ..
- Sorger D, Daum G. Triacylglycerol biosynthesis in yeast. Appl Microbiol Biotechnol. 2003;61:289-99 pubmed..The interplay of these organelles, formation of LP, and enzymatic properties of enzymes catalyzing the synthesis of PA, DAG, and TAG in yeast are discussed in this communication. ..
- Rajakumari S, Grillitsch K, Daum G. Synthesis and turnover of non-polar lipids in yeast. Prog Lipid Res. 2008;47:157-71 pubmed publisher..Moreover, we will discuss non-polar lipid homeostasis and its newly discovered links to various cell biological processes in the yeast. ..
- Steinboeck F, Bogusch A, Kaufmann A, Heidenreich E. The nuclear actin-related protein of Saccharomyces cerevisiae, Arp4, directly interacts with the histone acetyltransferase Esa1p. J Biochem. 2007;141:661-8 pubmed..We propose that the interaction of Arp4 with Esa1p is crucial for proper functioning and targeting of the NuA4 complex. ..
- Hanner M, Moebius F, Weber F, Grabner M, Striessnig J, Glossmann H. Phenylalkylamine Ca2+ antagonist binding protein. Molecular cloning, tissue distribution, and heterologous expression. J Biol Chem. 1995;270:7551-7 pubmed..EBP is suggested to be the first structurally characterized member of a family of high affinity microsomal drug acceptor proteins carrying so called sigma-binding sites...
- Waldherr M, Ragnini A, Jank B, Teply R, Wiesenberger G, Schweyen R. A multitude of suppressors of group II intron-splicing defects in yeast. Curr Genet. 1993;24:301-6 pubmed..This supports the notion that the MRS2 gene product is bifunctional i.e., it is essential for the splicing of group II introns and is also involved in processes of mitochondrial biogenesis other than RNA splicing. ..
- Kochl S, Fresser F, Lobentanz E, Baier G, Utermann G. Novel interaction of apolipoprotein(a) with beta-2 glycoprotein I mediated by the kringle IV domain. Blood. 1997;90:1482-9 pubmed..The apo(a)-beta2-glycoprotein I interaction indicates new potential roles for Lp(a) in fibrinolysis and autoimmunity. ..
- Mamnun Y, Pandjaitan R, Mahé Y, Delahodde A, Kuchler K. The yeast zinc finger regulators Pdr1p and Pdr3p control pleiotropic drug resistance (PDR) as homo- and heterodimers in vivo. Mol Microbiol. 2002;46:1429-40 pubmed
- Bürgermeister M, Birner Grünberger R, Nebauer R, Daum G. Contribution of different pathways to the supply of phosphatidylethanolamine and phosphatidylcholine to mitochondrial membranes of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2004;1686:161-8 pubmed..Thus, the different pathways of PtdEtn biosynthesis play different roles in the assembly of PtdEtn into cellular membranes...
- Schrettl M, Winkelmann G, Haas H. Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound. Biometals. 2004;17:647-54 pubmed..In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans...
- Petronczki M, Matos J, Mori S, Gregan J, Bogdanova A, Schwickart M, et al. Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1. Cell. 2006;126:1049-64 pubmed
- Schweiger W, Pasquet J, Nussbaumer T, Paris M, Wiesenberger G, Macadré C, et al. Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes. Mol Plant Microbe Interact. 2013;26:781-92 pubmed publisher..Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity. ..
- Benito Moreno R, Miaczynska M, Bauer B, Schweyen R, Ragnini A. Mrs6p, the yeast homologue of the mammalian choroideraemia protein: immunological evidence for its function as the Ypt1p Rab escort protein. Curr Genet. 1994;27:23-5 pubmed..These findings provide direct evidence for the role of Mrs6p as the REP component of the yeast Rab geranylgeranyl transferase enzyme. ..
- Brocard C, Kragler F, Simon M, Schuster T, Hartig A. The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL. Biochem Biophys Res Commun. 1994;204:1016-22 pubmed..Here we show that the PAS10 protein interacts in vivo with the C-terminal peroxisomal targeting signal. The part essential for this interaction contains the complete tetratricopeptide repeat domain. ..
- Lamping E, Luckl J, Paltauf F, Henry S, Kohlwein S. Isolation and characterization of a mutant of Saccharomyces cerevisiae with pleiotropic deficiencies in transcriptional activation and repression. Genetics. 1994;137:55-65 pubmed..Thus, Dep1p appears to be a new member of a class of transcriptional modulators, including Rpd1p/Sin3p/Ume4p/Sdi1p/Gam3p, Rpd3p, Spt10p and Spt21p. ..
- Bauer B, Lorenzetti S, Miaczynska M, Bui D, Schweyen R, Ragnini A. Amino- and carboxy-terminal domains of the yeast Rab escort protein are both required for binding of Ypt small G proteins. Mol Biol Cell. 1996;7:1521-33 pubmed..This analysis has shown that the SCRs are necessary but not sufficient for Ypt1p binding by the yeast REP, the carboxy-terminal region also being required. ..
- Barahmand Pour F, Meinke A, Groner B, Decker T. Jak2-Stat5 interactions analyzed in yeast. J Biol Chem. 1998;273:12567-75 pubmed..Therefore, the Jak kinase domain may be all that is needed to cause Stat phosphorylation in situations where receptor docking is dispensable. ..
- Potuschak T, Stary S, Schlögelhofer P, Becker F, Nejinskaia V, Bachmair A. PRT1 of Arabidopsis thaliana encodes a component of the plant N-end rule pathway. Proc Natl Acad Sci U S A. 1998;95:7904-8 pubmed..The RING finger domains have been found in a number of other proteins that are involved in ubiquitin conjugation, consistent with the proposed role of PRT1 in the plant N-end rule pathway. ..
- Bui D, Gregan J, Jarosch E, Ragnini A, Schweyen R. The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane. J Biol Chem. 1999;274:20438-43 pubmed..Mutants lacking or overproducing Mrs2p exhibit phenotypes consistent with the involvement of Mrs2p in mitochondrial Mg2+ homeostasis. ..
- Merkel O, Fido M, Mayr J, Prüger H, Raab F, Zandonella G, et al. Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae. J Biol Chem. 1999;274:28121-7 pubmed..Plb3p activity in a plb1 plb2 mutant background was observed in periplasmic space extracts. Both Plb3p and Plb2p display transacylase activity in vitro, in the presence or absence, respectively, of detergent. ..
- Wirth K, Ricci R, Giménez Abián J, Taghybeeglu S, Kudo N, Jochum W, et al. Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation. Genes Dev. 2004;18:88-98 pubmed..Re-entry into the cell cycle either occurred without any proliferative stimulus or could be easily induced. We demonstrate that the APC has an additional function to prevent hepatocytes from unscheduled re-entry into the cell cycle. ..
- Kraft C, Vodermaier H, Maurer Stroh S, Eisenhaber F, Peters J. The WD40 propeller domain of Cdh1 functions as a destruction box receptor for APC/C substrates. Mol Cell. 2005;18:543-53 pubmed..Our data imply that APC/C is activated by the association of Cdh1 with Cdc27, which enables APC/C to recognize the D box of substrates via Cdh1's propeller domain. ..
- Rinnerthaler M, Jarolim S, Heeren G, Palle E, Perju S, Klinger H, et al. MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria. Biochim Biophys Acta. 2006;1757:631-8 pubmed..Our results so far indicate that Mmi1p is one of the few proteins establishing a functional link between microtubules and mitochondria which may be needed for correct localization of mitochondria during cell division. ..
- Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, et al. The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell. 2007;19:2213-24 pubmed..cinerea. ..
- Daum G, Wagner A, Czabany T, Grillitsch K, Athenstaedt K. Lipid storage and mobilization pathways in yeast. Novartis Found Symp. 2007;286:142-51; discussion 151-4, 162-3, 196-203 pubmed..Consequently, regulation of neutral lipid metabolism does not only occur at the enzymatic level but also at the organelle level. ..
- Toshima J, Horikomi C, Okada A, Hatori M, Nagano M, Masuda A, et al. Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis. J Cell Sci. 2016;129:367-79 pubmed publisher..These results demonstrate the in vivo roles of Srv2p in the regulation of the actin cytoskeleton during clathrin-mediated endocytosis. ..
- Loidl J, Klein F, Scherthan H. Homologous pairing is reduced but not abolished in asynaptic mutants of yeast. J Cell Biol. 1994;125:1191-200 pubmed..This indicates that double-strand breaks are not an essential component of the meiotic homology searching mechanism or that there exist additional or alternative mechanisms for locating homologues. ..
- Moebius F, Fitzky B, Lee J, Paik Y, Glossmann H. Molecular cloning and expression of the human delta7-sterol reductase. Proc Natl Acad Sci U S A. 1998;95:1899-902 pubmed..013 microM), BM15766 (IC50 1.2 microM), and triparanol (IC50 14 microM). Our work paves the way to clarify whether a defect in the delta7-sterol reductase gene underlies the Smith-Lemli-Opitz syndrome. ..
- Gurvitz A, Langer S, Piskacek M, Hamilton B, Ruis H, Hartig A. Predicting the function and subcellular location of Caenorhabditis elegans proteins similar to Saccharomyces cerevisiae beta-oxidation enzymes. Yeast. 2000;17:188-200 pubmed
- Sorger D, Daum G. Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae. J Bacteriol. 2002;184:519-24 pubmed..In conclusion, at least three different pathways are involved in the formation of storage TAG in the yeast. ..
- Mendoza M, Hyman A, Glotzer M. GTP binding induces filament assembly of a recombinant septin. Curr Biol. 2002;12:1858-63 pubmed..If the properties of Xl Sept2 filaments reflect those of native septin complexes, these results imply that the growth or stability of septin filaments, or both, is regulated by the state of bound nucleotide. ..
- Nowikovsky K, Froschauer E, Zsurka G, Samaj J, Reipert S, Kolisek M, et al. The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome. J Biol Chem. 2004;279:30307-15 pubmed..Thus, YOL027/LETM1 are the first genes shown to encode factors involved in both K+ homeostasis and organelle volume control...
- Melikant B, Giuliani C, Halbmayer Watzina S, Limmongkon A, Heberle Bors E, Wilson C. The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13. FEBS Lett. 2004;576:5-8 pubmed..RT-PCR analysis showed the co-expression of these two genes in diverse plant tissues. These data show that AtMKK6 can functionally activate the MAP kinase AtMPK13. ..
- Kueng S, Hegemann B, Peters B, Lipp J, Schleiffer A, Mechtler K, et al. Wapl controls the dynamic association of cohesin with chromatin. Cell. 2006;127:955-67 pubmed..Wapl depletion also increases the residence time of cohesin on chromatin in interphase. Our data indicate that Wapl is required to unlock cohesin from a particular state in which it is stably bound to chromatin. ..
- Wiesenberger G, Speer F, Haller G, Bonnefoy N, Schleiffer A, Schafer B. RNA degradation in fission yeast mitochondria is stimulated by a member of a new family of proteins that are conserved in lower eukaryotes. J Mol Biol. 2007;367:681-91 pubmed
- Ruckenstuhl C, Poschenel A, Possert R, Baral P, Gruber K, Turnowsky F. Structure-function correlations of two highly conserved motifs in Saccharomyces cerevisiae squalene epoxidase. Antimicrob Agents Chemother. 2008;52:1496-9 pubmed publisher..In the homology model, these motifs flank the flavin adenine dinucleotide cofactor and form part of the interface between cofactor and substrate binding domains. ..
- Rajakumari S, Daum G. Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions. Mol Biol Cell. 2010;21:501-10 pubmed publisher..In summary, our results demonstrate that yeast Tgl3p and Tgl5p play a dual role in lipid metabolism contributing to both anabolic and catabolic processes. ..
- Panizza S, Mendoza M, Berlinger M, Huang L, Nicolas A, Shirahige K, et al. Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination. Cell. 2011;146:372-83 pubmed publisher..Loss of Rec114, Mer2, and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation. ..
- Kappel L, Loibl M, Zisser G, Klein I, Fruhmann G, Gruber C, et al. Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. J Cell Biol. 2012;199:771-82 pubmed publisher..Our results show that release of Rlp24 by Drg1 defines a key event in large subunit formation that is a prerequisite for progression of cytoplasmic pre-60S maturation. ..
- ..Molecular aspects of these processes as well as diseases related to defects in the formation of mitochondrial membranes will be described. ..
- Hofbauer H, Schopf F, Schleifer H, Knittelfelder O, Pieber B, Rechberger G, et al. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Dev Cell. 2014;29:729-39 pubmed publisher..These findings reveal an unexpected regulatory link between the major energy-sensing kinase, membrane lipid composition, and transcription. ..
- Gurvitz A, Wabnegger L, Langer S, Hamilton B, Ruis H, Hartig A. The tetratricopeptide repeat domains of human, tobacco, and nematode PEX5 proteins are functionally interchangeable with the analogous native domain for peroxisomal import of PTS1-terminated proteins in yeast. Mol Genet Genomics. 2001;265:276-86 pubmed..We reason that this is due to a cryptic PTS in native Eci1p that can function in a redundant system with the C-terminal HRL. ..
- Mitterbauer R, Karl T, Adam G. Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase. Appl Environ Microbiol. 2002;68:1336-43 pubmed..Growth can also be restored by expression of a human uridine phosphorylase cDNA. Yeast strains expressing protozoan N-ribohydrolases or host phosphorylases could therefore become useful tools in drug screens for specific inhibitors. ..
- Jungwirth H, Ring J, Mayer T, Schauer A, Buttner S, Eisenberg T, et al. Loss of peroxisome function triggers necrosis. FEBS Lett. 2008;582:2882-6 pubmed publisher..Thus, we conclude that loss of peroxisomal function leads to a form of necrotic cell death. ..
- Cvrckova F, Nasmyth K. Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation. EMBO J. 1993;12:5277-86 pubmed..This suggests that Cln1 and Cln2 may have a direct role in bud formation. ..
- Wintersberger U, Kuhne C, Karwan A. Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintenance of exact ploidy. Yeast. 1995;11:929-44 pubmed..Taken together the data suggest that the Scp160 protein is required during cell division for faithful partitioning of the ER-nuclear envelope membranes which in S. cerevisiae enclose the duplicated chromosomes. ..
- Hanner M, Moebius F, Flandorfer A, Knaus H, Striessnig J, Kempner E, et al. Purification, molecular cloning, and expression of the mammalian sigma1-binding site. Proc Natl Acad Sci U S A. 1996;93:8072-7 pubmed..Northern blots showed high densities of the sigma1-binding site mRNA in sterol-producing tissues. This is also in agreement with the known ability of sigma1-binding sites to interact with steroids, such as progesterone. ..
- Wolfger H, Mahe Y, Parle McDermott A, Delahodde A, Kuchler K. The yeast ATP binding cassette (ABC) protein genes PDR10 and PDR15 are novel targets for the Pdr1 and Pdr3 transcriptional regulators. FEBS Lett. 1997;418:269-74 pubmed
- Schneiter R, Guerra C, Lampl M, Gogg G, Kohlwein S, Klein H. The Saccharomyces cerevisiae hyperrecombination mutant hpr1Delta is synthetically lethal with two conditional alleles of the acetyl coenzyme A carboxylase gene and causes a defect in nuclear export of polyadenylated RNA. Mol Cell Biol. 1999;19:3415-22 pubmed
- Gregan J, Kolisek M, Schweyen R. Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo. Genes Dev. 2001;15:2229-37 pubmed..It explains finally why protein factors modulating Mg(2+) homeostasis had been identified in genetic screens for bona fide RNA splicing factors. ..
- Bauer B, Rossington D, Mollapour M, Mamnun Y, Kuchler K, Piper P. Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants. Eur J Biochem. 2003;270:3189-95 pubmed..These transporters do not confer resistance to high acetate levels and, in prototrophs, their presence is actually detrimental for this resistance...
- Katis V, Galova M, Rabitsch K, Gregan J, Nasmyth K. Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol. 2004;14:560-72 pubmed
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