Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Australia
Topic: saccharomyces cerevisiae
- Zaman M, Johnson A, Bobek G, Kueh S, Kersaitis C, Bailey T, et al. Protein kinase CK2 regulates metal toxicity in neuronal cells. Metallomics. 2016;8:82-90 pubmed publisher..Taken together, these findings shed light on a new facet of CK2 functionality and provide a basis for further research on the regulation of Zn(ii) and Ca(ii) homeostasis by CK2. ..
- Brennan T, Turner C, Krömer J, Nielsen L. Alleviating monoterpene toxicity using a two-phase extractive fermentation for the bioproduction of jet fuel mixtures in Saccharomyces cerevisiae. Biotechnol Bioeng. 2012;109:2513-22 pubmed publisher..Since farnesene is currently produced in S. cerevisiae, its use as a co-product and extractant for microbial terpene-based jet fuel production in a two-phase system offers an attractive bioprocessing option. ..
- Squires J, Preiss T. Function and detection of 5-methylcytosine in eukaryotic RNA. Epigenomics. 2010;2:709-15 pubmed publisher..In this article we review the occurrence, function and biochemical detection of m(5)C in eukaryotic RNA, and provide perspectives on the biological roles of this modification in the transcriptome. ..
- Hillman M, Gecz J. Fragile XE-associated familial mental retardation protein 2 (FMR2) acts as a potent transcription activator. J Hum Genet. 2001;46:251-9 pubmed..Elucidation of the function of the FMR2 protein as a transcription activator may place FMR2 within the molecular signalling pathways involved in nonspecific X-linked mental retardation (MRX). ..
- Maleszka R, de Couet H, Miklos G. Data transferability from model organisms to human beings: insights from the functional genomics of the flightless region of Drosophila. Proc Natl Acad Sci U S A. 1998;95:3731-6 pubmed
- Allison L, Edgoose T, Dix T. Compression of strings with approximate repeats. Proc Int Conf Intell Syst Mol Biol. 1998;6:8-16 pubmed..The model is further extended to include approximate reverse complementary repeats when analyzing DNA strings. Tests include the recovery of parameter estimates from known sources and applications to real DNA strings. ..
- Holt S, Cordente A, Williams S, Capone D, Jitjaroen W, Menz I, et al. Engineering Saccharomyces cerevisiae to release 3-Mercaptohexan-1-ol during fermentation through overexpression of an S. cerevisiae Gene, STR3, for improvement of wine aroma. Appl Environ Microbiol. 2011;77:3626-32 pubmed publisher..These data provide direct evidence for a yeast enzyme able to release aromatic thiols in vitro that can be applied in the development of self-cloned yeast to enhance wine flavor. ..
- Paulsen I, Skurray R. The POT family of transport proteins. Trends Biochem Sci. 1994;19:404 pubmed
- Couttas T, Raftery M, Padula M, Herbert B, Wilkins M. Methylation of translation-associated proteins in Saccharomyces cerevisiae: Identification of methylated lysines and their methyltransferases. Proteomics. 2012;12:960-72 pubmed publisher..We suggest that enzyme YBR271W is named EF methyltransferase 2 (Efm2), in line with the recent naming of YHL039W as Efm1. ..
- Gauci V, Beckhouse A, Lyons V, Beh E, Rogers P, Dawes I, et al. Zinc starvation induces a stress response in Saccharomyces cerevisiae that is mediated by the Msn2p and Msn4p transcriptional activators. FEMS Yeast Res. 2009;9:1187-95 pubmed publisher..This research provides a better understanding of the molecular mechanisms involved in the yeast response to zinc deficiency during fermentation. ..
- Tsoi B, Beckhouse A, Gelling C, Raftery M, Chiu J, Tsoi A, et al. Essential role of one-carbon metabolism and Gcn4p and Bas1p transcriptional regulators during adaptation to anaerobic growth of Saccharomyces cerevisiae. J Biol Chem. 2009;284:11205-15 pubmed publisher..During anaerobic starvation for l-serine, this essential amino acid is preferentially directed to the cell wall, indicating the existence of a regulatory mechanism to balance competing cellular demands. ..
- Smith F, Ealing P, Dong B, Delhaize E. The cloning of two Arabidopsis genes belonging to a phosphate transporter family. Plant J. 1997;11:83-92 pubmed..The APT1 and APT2 polypeptides are likely to be associated with the membrane transport of phosphate within A. thaliana roots. ..
- Dickinson J, Dawes I. The catabolism of branched-chain amino acids occurs via 2-oxoacid dehydrogenase in Saccharomyces cerevisiae. J Gen Microbiol. 1992;138:2029-33 pubmed..These results suggest that in S. cerevisiae the catabolism of branched-chain amino acids occurs via 2-oxoacid dehydrogenase, not via the 'Ehrlich Pathway'. ..
- Byrne J, Nourse C, Basset P, Gunning P. Identification of homo- and heteromeric interactions between members of the breast carcinoma-associated D52 protein family using the yeast two-hybrid system. Oncogene. 1998;16:873-81 pubmed..Thus, D52-like proteins appear to exert and/or regulate their activities through specific interactions with other D52-like proteins, which in turn may be intrinsic to potential roles of these molecules in controlling cell proliferation. ..
- Swiegers J, Pretorius I. Modulation of volatile sulfur compounds by wine yeast. Appl Microbiol Biotechnol. 2007;74:954-60 pubmed
- Bellon J, Schmid F, Capone D, Dunn B, Chambers P. Introducing a new breed of wine yeast: interspecific hybridisation between a commercial Saccharomyces cerevisiae wine yeast and Saccharomyces mikatae. PLoS ONE. 2013;8:e62053 pubmed publisher..The new S. cerevisiae x S. mikatae hybrids have the potential to produce complex wines akin to products of spontaneous fermentation while giving winemakers the safeguard of an inoculated ferment...
- Williams T, Nielsen L, Vickers C. Engineered quorum sensing using pheromone-mediated cell-to-cell communication in Saccharomyces cerevisiae. ACS Synth Biol. 2013;2:136-49 pubmed publisher..These programs could be used to control biochemical pathways for the production of fuels and chemicals that are toxic or place a heavy metabolic burden on cell growth. ..
- Gear M, McPhillips M, Patrick J, McCurdy D. Hexose transporters of tomato: molecular cloning, expression analysis and functional characterization. Plant Mol Biol. 2000;44:687-97 pubmed..LeHT2-dependent transport of glucose in RE700A exhibited properties consistent with the operation of an energy-coupled transporter and probably a H+/hexose symporter. The Km of the symporter for glucose is 45 microM. ..
- Delhaize E, Hebb D, Richards K, Lin J, Ryan P, Gardner R. Cloning and expression of a wheat (Triticum aestivum L.) phosphatidylserine synthase cDNA. Overexpression in plants alters the composition of phospholipids. J Biol Chem. 1999;274:7082-8 pubmed..The cloning of TaPSS1 now provides evidence that the yeast pathway for PS synthesis exists in some plant tissues and provides a tool for understanding the pathways of phospholipid biosynthesis and their regulation in plants...
- Zhang L, Koyyalamudi S, Jeong S, Reddy N, Smith P, Ananthan R, et al. Antioxidant and immunomodulatory activities of polysaccharides from the roots of Sanguisorba officinalis. Int J Biol Macromol. 2012;51:1057-62 pubmed publisher..These results suggest that the roots of S. officinalis are likely to have therapeutic value for the treatment of cancer...
- Winter G, Curtin C. In situ high throughput method for H(2)S detection during micro-scale wine fermentation. J Microbiol Methods. 2012;91:165-70 pubmed publisher..The method, validated here for Saccharomyces cerevisiae, is suited for high throughput screening purposes by virtue of its simplicity and the ability to detect H(2)S in micro-scale fermentations. ..
- Biswas C, Djordjevic J, Zuo X, Boles E, Jolliffe K, Sorrell T, et al. Functional characterization of the hexose transporter Hxt13p: an efflux pump that mediates resistance to miltefosine in yeast. Fungal Genet Biol. 2013;61:23-32 pubmed publisher..In summary, we have identified a new function of the hexose sugar transporter gene HXT13 when overexpressed in S. cerevisiae, namely, in efflux of MI and in mediating MI resistance. ..
- Shewan A, McCann R, Lamb C, Stirrat L, Kioumourtzoglou D, Adamson I, et al. Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells. J Cell Sci. 2013;126:1576-82 pubmed publisher..These findings demonstrate that the trafficking signals and their cognate molecular regulatory machinery that mediate regulated exocytosis of membrane proteins are conserved across evolution. ..
- Padman B, Bach M, Lucarelli G, Prescott M, Ramm G. The protonophore CCCP interferes with lysosomal degradation of autophagic cargo in yeast and mammalian cells. Autophagy. 2013;9:1862-75 pubmed publisher..In addition, our data demonstrated that caution should be taken when using organelle-specific dyes in conjunction with strategies affecting membrane potential. ..
- Fong C, Temple M, Alic N, Chiu J, Durchdewald M, Thorpe G, et al. Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor. FEMS Yeast Res. 2008;8:386-99 pubmed publisher..These data show that functions such as heat shock response, and glucose transport are involved in the response. ..
- Poole K, E Walker M, Warren T, Gardner J, McBryde C, de Barros Lopes M, et al. Proline transport and stress tolerance of ammonia-insensitive mutants of the PUT4-encoded proline-specific permease in yeast. J Gen Appl Microbiol. 2009;55:427-39 pubmed..This increase in proline accumulation was associated with increased cell viability in conditions of high temperature and osmotic stress raising possible benefits in industrial fermentation applications. ..
- Smith F, Hawkesford M, Prosser I, Clarkson D. Isolation of a cDNA from Saccharomyces cerevisiae that encodes a high affinity sulphate transporter at the plasma membrane. Mol Gen Genet. 1995;247:709-15 pubmed..We conclude that the SUL1 cDNA encodes a S. cerevisiae high affinity sulphate transporter that is responsible for the transfer of sulphate across the plasma membrane from the external medium. ..
- Brewster N, Val D, Walker M, Wallace J. Regulation of pyruvate carboxylase isozyme (PYC1, PYC2) gene expression in Saccharomyces cerevisiae during fermentative and nonfermentative growth. Arch Biochem Biophys. 1994;311:62-71 pubmed..This study represents the first report on the characterisation of expression of the PYC genes in yeast throughout growth. Their metabolic roles for both fermentative and gluconeogenic growth are considered. ..
- Gurvitz A, Rottensteiner H, Kilpeläinen S, Hartig A, Hiltunen J, Binder M, et al. The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19. J Biol Chem. 1997;272:22140-7 pubmed..SPS19 is dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate. ..
- Kaiser B, Finnegan P, Tyerman S, Whitehead L, Bergersen F, Day D, et al. Characterization of an ammonium transport protein from the peribacteroid membrane of soybean nodules. Science. 1998;281:1202-6 pubmed..GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host...
- van Vliet J, Turner J, Crossley M. Human Krüppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription. Nucleic Acids Res. 2000;28:1955-62 pubmed..Finally we determine that KLF8 is broadly expressed in human tissues. These results establish KLF8 as a CACCC-box binding protein that associates with CtBP and represses transcription. ..
- Eglinton J, Heinrich A, Pollnitz A, Langridge P, Henschke P, de Barros Lopes M. Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene. Yeast. 2002;19:295-301 pubmed..The implications for the use of these modifications in commercial wine production require further investigation in wine yeast strains. ..
- Senapin S, Clark Walker G, Chen X, Seraphin B, Daugeron M. RRP20, a component of the 90S preribosome, is required for pre-18S rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2003;31:2524-33 pubmed..As the protein encoded by YOR145c is found in pre-ribosomal particles and the mutant strain is defective in ribosomal RNA processing, we have renamed it as RRP20. ..
- Senapin S, Chen X, Clark Walker G. Transcription of TIM9, a new factor required for the petite-positive phenotype of Saccharomyces cerevisiae, is defective in spt7 mutants. Curr Genet. 2003;44:202-10 pubmed
- Gecz J, Shaw M, Bellon J, de Barros Lopes M. Human wild-type SEDL protein functionally complements yeast Trs20p but some naturally occurring SEDL mutants do not. Gene. 2003;320:137-44 pubmed
- Jones P, Turner K, Djordjevic J, Sorrell T, Wright L, George A. Role of conserved active site residues in catalysis by phospholipase B1 from Cryptococcus neoformans. Biochemistry. 2007;46:10024-32 pubmed publisher..These results provide an explanation for differences in substrate specificity between lipases sharing the cPLA2 catalytic domain fold and for the differential effect of inhibitors on PLB1 enzymatic activities...
- Fitzpatrick K, Tyerman S, Kaiser B. Molybdate transport through the plant sulfate transporter SHST1. FEBS Lett. 2008;582:1508-13 pubmed publisher..Uptake was not inhibited by sulfate, but sulfate transport via SHST1 was reduced with molybdate. These results are the first measurement of molybdate transport by a characterised plant sulfate transport protein...
- McCurdy D, Dibley S, Cahyanegara R, Martin A, Patrick J. Functional characterization and RNAi-mediated suppression reveals roles for hexose transporters in sugar accumulation by tomato fruit. Mol Plant. 2010;3:1049-63 pubmed publisher..Collectively, these findings demonstrate that LeHTs play key roles in driving accumulation of hexoses into storage parenchyma cells during tomato fruit development...
- Diefenbach R, Diefenbach E, Douglas M, Cunningham A. The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23. Biochemistry. 2002;41:14906-15 pubmed..In support of separate binding sites on heavy chain for light chain and SNAPs, a complex of heavy and light chains was observed to interact with SNAP25 and SNAP23. ..
- Gaspar Y, Johnson K, McKenna J, Bacic A, Schultz C. The complex structures of arabinogalactan-proteins and the journey towards understanding function. Plant Mol Biol. 2001;47:161-76 pubmed..Here we examine models for proteoglycan function in animals and yeast to highlight possible strategies for determining the function(s) of individual AGPs in plants. ..
- Kucharski R, Maleszka R. Transcriptional profiling reveals multifunctional roles for transferrin in the honeybee, Apis mellifera. J Insect Sci. 2003;3:27 pubmed..We conclude that major fluctuations of AmTRF mRNA in time and space are consistent with context-dependent functional significance and suggest broader multifunctional roles for transferrin in insects. ..
- Gregory M, Cleland L, James M. Molecular basis for differential elongation of omega-3 docosapentaenoic acid by the rat Elovl5 and Elovl2. J Lipid Res. 2013;54:2851-7 pubmed publisher..Understanding the ability of elongases to synthesize 24:5n-3 may provide a basis for using sequence data to predict their ability to ultimately support DHA synthesis. ..
- Val D, Chapman Smith A, Walker M, Cronan J, Wallace J. Polymorphism of the yeast pyruvate carboxylase 2 gene and protein: effects on protein biotinylation. Biochem J. 1995;312 ( Pt 3):817-25 pubmed..The K1155R substitution had very little effect on biotinylation, but the five-amino-acid C-terminal extension to Pyc2 and the N-terminal extension to Pycl both improved biotinylation in vivo. ..
- Sinclair D, Dawes I. Genetics of the synthesis of serine from glycine and the utilization of glycine as sole nitrogen source by Saccharomyces cerevisiae. Genetics. 1995;140:1213-22 pubmed..Representatives from three complementation groups were also unable to grow on glycine as sole nitrogen source (gsd1-3). Assays of the rate of glycine uptake and decarboxylation have provided insights into the nature of the mutations. ..