Genomes and Genes
Experts and Doctors on rna in United States
Locale: United States
Publications558 found, 100 shown here
- Liu H, Trinh T, Dong H, Keith R, Nelson D, Liu C. Iron regulator hepcidin exhibits antiviral activity against hepatitis C virus. PLoS ONE. 2012;7:e46631 pubmed publisher..In conclusion, hepcidin can induce intracellular antiviral state while HCV has a strategy to suppress hepcidin expression. This may be a novel mechanism by which HCV circumvents hepatic innate antiviral defense. ..
- Jones C, Saadatmand J, Kleiman L, Musier Forsyth K. Molecular mimicry of human tRNALys anti-codon domain by HIV-1 RNA genome facilitates tRNA primer annealing. RNA. 2013;19:219-29 pubmed publisher..Our results suggest that HIV-1 uses molecular mimicry of the anti-codon of tRNA(Lys) to increase the efficiency of tRNA(Lys3) annealing to viral RNA. ..
- Koeth R, Wang Z, Levison B, Buffa J, Org E, Sheehy B, et al. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat Med. 2013;19:576-85 pubmed publisher..Intestinal microbiota may thus contribute to the well-established link between high levels of red meat consumption and CVD risk...
- Iacovelli J, Rowe G, Khadka A, Diaz Aguilar D, Spencer C, Arany Z, et al. PGC-1Î± Induces Human RPE Oxidative Metabolism and Antioxidant Capacity. Invest Ophthalmol Vis Sci. 2016;57:1038-51 pubmed publisher..This study provides important insights into the metabolic changes associated with RPE functional maturation and identifies PGC-1Î± as a potent driver of RPE mitochondrial function and antioxidant capacity. ..
- Zu T, Cleary J, Liu Y, Bañez Coronel M, Bubenik J, Ayhan F, et al. RAN Translation Regulated by Muscleblind Proteins in Myotonic Dystrophy Type 2. Neuron. 2017;95:1292-1305.e5 pubmed publisher..video abstract. ..
- Chang P, Dignam J. Primary structure of alanyl-tRNA synthetase and the regulation of its mRNA levels in Bombyx mori. J Biol Chem. 1990;265:20898-906 pubmed..Genomic Southern blot analysis using a nick-translated cDNA probe revealed hybridization to single fragments when B. mori genomic DNA was digested with various restriction endonucleases. ..
- Kadowaki T, Goldfarb D, Spitz L, Tartakoff A, Ohno M. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO J. 1993;12:2929-37 pubmed..Thus, MTR1 and its homologs appear to act as GNRPs for a family of conserved GTPases in controlling RNA metabolism and transport. Their role in governing checkpoint control appears to be restricted to higher eukaryotes. ..
- Denisenko O, Shnyreva M, Suzuki H, Bomsztyk K. Point mutations in the WD40 domain of Eed block its interaction with Ezh2. Mol Cell Biol. 1998;18:5634-42 pubmed..These findings suggest that Polycomb group proteins Eed and Ezh2 functionally interact in mammalian cells, an interaction that is mediated by the WD40-containing domain of Eed protein. ..
- Provenzano P, Alejandro Osorio A, Valhmu W, Jensen K, Vanderby R. Intrinsic fibroblast-mediated remodeling of damaged collagenous matrices in vivo. Matrix Biol. 2005;23:543-55 pubmed..As such, this system provides unique insight into acute tissue damage and offers a potentially powerful model to examine fibroblast behavior. ..
- Hoang C, Chen J, Vizthum C, Kandel J, Hamilton C, Mueller E, et al. Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure. Mol Cell. 2006;24:535-45 pubmed..Sequence and structural comparisons suggest that pseudouridine synthases of the RluA, RsuA, and TruA families employ an equivalent arginine for base flipping. ..
- Kurtzman C, Robnett C, Basehoar Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res. 2008;8:939-54 pubmed publisher..Lineages previously classified as varieties of Pichia kluyveri, 'Issatchenkia'scutulata, Starmera amethionina and 'Williopsis'saturnus are elevated to species rank based on sequence comparisons...
- Chadalavada D, Bevilacqua P. Analyzing RNA and DNA folding using temperature gradient gel electrophoresis (TGGE) with application to in vitro selections. Methods Enzymol. 2009;468:389-408 pubmed publisher..Specific examples are chosen from the literature to illustrate the methods. TGGE provides a powerful biophysical approach for analyzing RNA and DNA that complements more traditional methodologies. ..
- Bitel C, Singh V, Frederikse P. miR-124, miR-125b, let-7 and vesicle transport proteins in squid lenses in L. pealei. Curr Eye Res. 2012;37:388-94 pubmed publisher..Our study provided evidence that miR-124, miR-125, and let-7, as well as proteins involved in vesicle transport linked with synaptic and cargo vesicle transport in vertebrates are also expressed in squid lenses. ..
- Carrocci T, Hoskins A. Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores. Analyst. 2014;139:44-7 pubmed publisher..These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range. ..
- Post T, Liszewski M, Adams E, Tedja I, Miller E, Atkinson J. Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype. J Exp Med. 1991;174:93-102 pubmed..The expression in a single cell of four distinct protein species with variable STP-rich regions and cytoplasmic tails represents an interesting example of the use of alternative splicing to provide variability in a mammalian protein. ..
- Takahara K, Kessler E, Biniaminov L, Brusel M, Eddy R, Jani Sait S, et al. Type I procollagen COOH-terminal proteinase enhancer protein: identification, primary structure, and chromosomal localization of the cognate human gene (PCOLCE). J Biol Chem. 1994;269:26280-5 pubmed..The human enhancer gene, PCOLCE, is localized to 7q21.3-->q22, the same chromosomal region containing the type I collagen alpha 2 chain gene, COL1A2. ..
- Yin G, Liu W, An P, Li P, Ding I, Planelles V, et al. Endostatin gene transfer inhibits joint angiogenesis and pannus formation in inflammatory arthritis. Mol Ther. 2002;5:547-54 pubmed..These data suggest a novel mechanism by which endostatin inhibits angiogenesis, and demonstrates the potential utility of anti-angiogenic gene therapy for treatment of inflammatory arthritis. ..
- London L, Majeski E, Altman Hamamdzic S, Enockson C, Paintlia M, Harley R, et al. Respiratory reovirus 1/L induction of diffuse alveolar damage: pulmonary fibrosis is not modulated by corticosteroids in acute respiratory distress syndrome in mice. Clin Immunol. 2002;103:284-95 pubmed..The histopathology, cytokine/chemokine expression, and response to corticosteroids in reovirus 1/L-induced ARDS are similar to what is observed in human patients, making this a clinically relevant model...
- Rose J, Parker W, Someya H, Shaddix S, Montgomery J, Secrist J. Enhancement of nucleoside cytotoxicity through nucleotide prodrugs. J Med Chem. 2002;45:4505-12 pubmed..Further cell culture experiments were conducted to gain insight into the mechanisms of cytotoxicity of these two prodrugs, and those data are reported. ..
- Zhao Y, Baranger A. Design of an adenosine analogue that selectively improves the affinity of a mutant U1A protein for RNA. J Am Chem Soc. 2003;125:2480-8 pubmed..Experiments with other U1A mutant proteins suggest that the stabilization of the complex between the Phe56Ala U1A protein and stem loop 2 RNA is due to a specific interaction between the Phe56Ala U1A protein and A6-4CPh stem loop 2 RNA. ..
- Kuziel W, Dawson T, Quinones M, Garavito E, Chenaux G, Ahuja S, et al. CCR5 deficiency is not protective in the early stages of atherogenesis in apoE knockout mice. Atherosclerosis. 2003;167:25-32 pubmed..These results indicate that the early stages of plaque formation in this model of lipid-mediated atherogenesis do not depend on CCR5. ..
- Rhee S, Burke D. Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistry. Anal Biochem. 2004;325:137-43 pubmed..Under these same conditions, the 5(')-thiophosphate showed negligible decay, and TCEP was more stable than DTT. TCEP thermostability was equivalent in the presence of 5 or 50mM MgCl(2) and 10mM adenosine or ATP. ..
- Anderson A, Schein T, Kalapila A, Lai L, Waldrop Valverde D, Moore R, et al. Soluble membrane attack complex in the blood and cerebrospinal fluid of HIV-infected individuals, relationship to HIV RNA, and comparison with HIV negatives. J Neuroimmunol. 2017;311:35-39 pubmed publisher..In HIV+ participants, there were negative association trends between sMAC and neurocognitive performance but these did not reach statistical significance. ..
- Ford V, Stringer C, Kennel S. Thrombomodulin is preferentially expressed in Balb/c lung microvessels. J Biol Chem. 1992;267:5446-50 pubmed..It is suggested that expression of TM is highest in cells from continuous endothelium. ..
- Johnson K, Smith K. Molecular cloning of a novel human cdc2/CDC28-like protein kinase. J Biol Chem. 1991;266:3402-7 pubmed
- Kelley R. Initial organization of the Drosophila dorsoventral axis depends on an RNA-binding protein encoded by the squid gene. Genes Dev. 1993;7:948-60 pubmed..A model of D/V axis formation is presented postulating that squid is needed to organize a concentration gradient of a morphogen originating in the germinal vesicle. ..
- Parsch J, Braverman J, Stephan W. Comparative sequence analysis and patterns of covariation in RNA secondary structures. Genetics. 2000;154:909-21 pubmed..This result is in qualitative agreement with Kimura's model of compensatory fitness interactions, which assumes that mutations occurring in RNA helices are individually deleterious but become neutral in appropriate combinations. ..
- Roebuck B, Curphey T, Li Y, Baumgartner K, Bodreddigari S, Yan J, et al. Evaluation of the cancer chemopreventive potency of dithiolethione analogs of oltipraz. Carcinogenesis. 2003;24:1919-28 pubmed..While efficacy in humans has yet to be tested, D3T is clearly more potent than oltipraz and serves as a useful molecular probe for determining the key events associated with protection by this class of agents. ..
- Agarwal A, Munoz Najar U, Klueh U, Shih S, Claffey K. N-acetyl-cysteine promotes angiostatin production and vascular collapse in an orthotopic model of breast cancer. Am J Pathol. 2004;164:1683-96 pubmed..These results suggest that systemic NAC therapy promotes anti-angiogenesis through angiostatin production, resulting in endothelial apoptosis and vascular collapse in the tumor. ..
- Maiti S, Grant S, Baker S, Karanth S, Pope C, Chen G. Stress regulation of sulfotransferases in male rat liver. Biochem Biophys Res Commun. 2004;323:235-41 pubmed..This suggests a stress regulation mechanism of SULT2A1 at the gene transcription level, possibly occurring via hormones. ..
- Mower J. PREP-Mt: predictive RNA editor for plant mitochondrial genes. BMC Bioinformatics. 2005;6:96 pubmed..The PREP-Mt methodology should be applicable to any system where RNA editing increases protein conservation across species. ..
- Wang W, Dimatteo D, Funanage V, Scavina M. Increased susceptibility of spinal muscular atrophy fibroblasts to camptothecin-induced cell death. Mol Genet Metab. 2005;85:38-45 pubmed..Our data demonstrate that SMA fibroblasts are more prone to some, but not all, death-stimuli. Vulnerability to death-stimuli is associated with decreased levels of SMN protein and is mediated by activation of caspase-3. ..
- Codrea V, Hayner M, Hall B, Jhaveri S, Ellington A. In vitro selection of RNA aptamers to a small molecule target. Curr Protoc Nucleic Acid Chem. 2010;Chapter 9:Unit 9.5.1-23 pubmed publisher..This unit describes two modes of selection, one by column filtration and one by batch selection. ..
- Yuan C, Liu Y, Yang M, Liao D. New methods as alternative or corrective measures for the pitfalls and artifacts of reverse transcription and polymerase chain reactions (RT-PCR) in cloning chimeric or antisense-accompanied RNA. RNA Biol. 2013;10:958-67 pubmed publisher..The ERP and overlapping antisense together set complex pitfalls, which one should be aware of. ..
- Voigts Hoffmann F, Schmitz N, Shen K, Shan S, Ataide S, Ban N. The structural basis of FtsY recruitment and GTPase activation by SRP RNA. Mol Cell. 2013;52:643-54 pubmed publisher..The complete conservation of the key residues of the SRP-RNA and the SRP protein implies that the suggested chemical mechanism of GTPase activation is applicable across all kingdoms. ..
- Stewart J, Subramanian H, Franco E. Self-assembly of multi-stranded RNA motifs into lattices and tubular structures. Nucleic Acids Res. 2017;45:5449-5457 pubmed publisher..These large assemblies could be potentially integrated with a variety of functional RNA motifs for drug or nanoparticle delivery, or for colocalization of cellular components. ..
- Zhao X, Li Z, Terns R, Terns M, Yu Y. An H/ACA guide RNA directs U2 pseudouridylation at two different sites in the branchpoint recognition region in Xenopus oocytes. RNA. 2002;8:1515-25 pubmed..Our results clarify the mechanism of U2 snRNA pseudouridylation in Xenopus oocytes, and have interesting implications with regard to the intranuclear localization of U2 snRNA pseudouridylation. ..
- Aldred M, Morrison C, Gimm O, Hoang Vu C, Krause U, Dralle H, et al. Peroxisome proliferator-activated receptor gamma is frequently downregulated in a diversity of sporadic nonmedullary thyroid carcinomas. Oncogene. 2003;22:3412-6 pubmed
- Shajani Z, Drobny G, Varani G. Binding of U1A protein changes RNA dynamics as observed by 13C NMR relaxation studies. Biochemistry. 2007;46:5875-83 pubmed..Together with previous studies of the RNA-bound protein, they also demonstrate that protein-RNA interfaces experience complex motions that modulate the strength of individual interactions. ..
- Tijerina P, Mohr S, Russell R. DMS footprinting of structured RNAs and RNA-protein complexes. Nat Protoc. 2007;2:2608-23 pubmed..This protocol takes 1.5-3 d to complete, depending on the type of analysis used. ..
- Wang K, Li H, Yuan Y, Etheridge A, Zhou Y, Huang D, et al. The complex exogenous RNA spectra in human plasma: an interface with human gut biota?. PLoS ONE. 2012;7:e51009 pubmed publisher..These findings raise the possibility that plasma RNAs of exogenous origin may serve as signaling molecules mediating for example the human-microbiome interaction and may affect and/or indicate the state of human health. ..
- Doherty K, Wappel R, TALBERT D, Trusk P, Moran D, KRAMER J, et al. Multi-parameter in vitro toxicity testing of crizotinib, sunitinib, erlotinib, and nilotinib in human cardiomyocytes. Toxicol Appl Pharmacol. 2013;272:245-55 pubmed publisher..This study demonstrates that a multi-parameter approach can provide a robust characterization of drug-induced cardiomyocyte damage that can be leveraged to improve drug safety during early phase development. ..
- Haas B, Papanicolaou A, Yassour M, Grabherr M, Blood P, Bowden J, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc. 2013;8:1494-512 pubmed publisher..sourceforge.net. The run time of this protocol is highly dependent on the size and complexity of data to be analyzed. The example data set analyzed in the procedure detailed herein can be processed in less than 5 h. ..
- Dhahbi J, Spindler S, Atamna H, Boffelli D, Mote P, Martin D. 5'-YRNA fragments derived by processing of transcripts from specific YRNA genes and pseudogenes are abundant in human serum and plasma. Physiol Genomics. 2013;45:990-8 pubmed publisher..The function of the 5'-YRNA fragments is at present unknown, but the processing and secretion of specific YRNAs to produce 5'-end fragments that circulate in stable complexes are consistent with a signaling function. ..
- Hagman D, Hays L, Parazzoli S, Poitout V. Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans. J Biol Chem. 2005;280:32413-8 pubmed..These results demonstrate that prolonged exposure of islets to palmitate inhibits insulin gene transcription by impairing nuclear localization of PDX-1 and cellular expression of MafA. ..
- Abdelsamie S, Li Y, Huang Y, Lee M, Klein R, Virella G, et al. Oxidized LDL immune complexes stimulate collagen IV production in mesangial cells via Fc gamma receptors I and III. Clin Immunol. 2011;139:258-66 pubmed publisher..Taken together, this study showed that oxLDL-IC stimulated collagen IV in mesangial cells via Fc?RI and Fc?RIII, and the expression of Fc?RI was increased by oxLDL-IC. ..
- Bansal A, Lee I, Hong X, Mathur S, Tawfik O, Rastogi A, et al. Discovery and validation of Barrett's esophagus microRNA transcriptome by next generation sequencing. PLoS ONE. 2013;8:e54240 pubmed publisher..Expression of miRNA between BE human tissues and BE cell lines are highly correlated. These miRNA should be studied in biological models to further understand BE development. ..
- Cui X, Navneet S, Wang J, Roon P, Chen W, Xian M, et al. Analysis of MTHFR, CBS, Glutathione, Taurine, and Hydrogen Sulfide Levels in Retinas of Hyperhomocysteinemic Mice. Invest Ophthalmol Vis Sci. 2017;58:1954-1963 pubmed publisher..Elevation of H2S is particularly intriguing owing to neuroprotective properties reported for this gasotransmitter. ..
- Lima W, Crooke S. Binding affinity and specificity of Escherichia coli RNase H1: impact on the kinetics of catalysis of antisense oligonucleotide-RNA hybrids. Biochemistry. 1997;36:390-8 pubmed..These results offer further insights into the physical binding properties of the RNase H-substrate interaction as well as the design of effective antisense oligonucleotides. ..
- Yan W, Lewin A, Hauswirth W. Selective degradation of nonsense beta-phosphodiesterase mRNA in the heterozygous rd mouse. Invest Ophthalmol Vis Sci. 1998;39:2529-36 pubmed..This allele-specific degradation serves to decrease mutant transcript levels dramatically in all rd strains, and suggests that photoreceptor cells have the capacity to reduce the level of an mRNA containing a nonsense mutation. ..
- Song K, Takemaru K, Moon R. A role for xGCNF in midbrain-hindbrain patterning in Xenopus laevis. Dev Biol. 1999;213:170-9 pubmed
- Wilson E, Hsieh M, Rotwein P. Autocrine growth factor signaling by insulin-like growth factor-II mediates MyoD-stimulated myocyte maturation. J Biol Chem. 2003;278:41109-13 pubmed..Our observations identify a robust autocrine amplification network in which MyoD enhances the later steps in muscle differentiation by induction of a locally acting growth factor. ..
- Fang X, Han H, Stamatoyannopoulos G, Li Q. Developmentally specific role of the CCAAT box in regulation of human gamma-globin gene expression. J Biol Chem. 2004;279:5444-9 pubmed..Our data also suggest that it is unlikely that transcriptional stimulation by the CCAAT box is exerted through direct protein-protein interaction between NF-Y and TBP. ..
- Ling J, Ainol L, Zhang L, Yu X, Pi W, Tuan D. HS2 enhancer function is blocked by a transcriptional terminator inserted between the enhancer and the promoter. J Biol Chem. 2004;279:51704-13 pubmed
- Nikrad M, Johnson T, Puthalalath H, Coultas L, Adams J, Kraft A. The proteasome inhibitor bortezomib sensitizes cells to killing by death receptor ligand TRAIL via BH3-only proteins Bik and Bim. Mol Cancer Ther. 2005;4:443-9 pubmed..These results implicate BH3-only proteins, in particular both Bik and Bim, as important mediators of the antitumor action of bortezomib and establish their role in its enhancement of TRAIL-induced apoptosis. ..
- Box J, Bunch J, Tang W, Baumann P. Spliceosomal cleavage generates the 3' end of telomerase RNA. Nature. 2008;456:910-4 pubmed publisher..We establish that 3' end processing of TER1 is critical for telomerase function and describe a previously unknown mechanism for RNA maturation that uses the ability of the spliceosome to mediate site-specific cleavage. ..
- Reveal B, Garcia C, Ellington A, Macdonald P. Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression. RNA Biol. 2011;8:1047-60 pubmed publisher..Other proteins with multiple RRMs may employ combinatorial binding to achieve high levels of specificity and affinity. ..
- Zou F, Chai H, Younkin C, Allen M, Crook J, Pankratz V, et al. Brain expression genome-wide association study (eGWAS) identifies human disease-associated variants. PLoS Genet. 2012;8:e1002707 pubmed publisher..Our findings have implications for the design and interpretation of eGWAS in general and the use of brain expression quantitative trait loci in the study of human disease genetics. ..
- Alagappan D, Balan M, Jiang Y, Cohen R, Kotenko S, Levison S. Egr-1 is a critical regulator of EGF-receptor-mediated expansion of subventricular zone neural stem cells and progenitors during recovery from hypoxia-hypoglycemia. ASN Neuro. 2013;5:183-93 pubmed publisher..They also provide new information into mechanisms that promote NP expansion and provide insights into strategies for amplifying the numbers of stem cells for CNS (central nervous system) regeneration. ..
- Lienert F, Lohmueller J, Garg A, Silver P. Synthetic biology in mammalian cells: next generation research tools and therapeutics. Nat Rev Mol Cell Biol. 2014;15:95-107 pubmed publisher..Synthetic biology is also leading to innovative therapeutic interventions based on cell-based therapies, protein drugs, vaccines and gene therapies. ..
- Scotton C, Bovolenta M, Schwartz E, Falzarano M, Martoni E, Passarelli C, et al. Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy. J Cell Sci. 2016;129:1671-84 pubmed publisher..It also opens new avenues for therapies of hereditary myopathies to modulate the molecular clock or potential gene-environment interactions that might modify muscle damage pathogenesis. ..
- Cali J, Balcueva E, Rybalkin I, Robishaw J. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning. J Biol Chem. 1992;267:24023-7 pubmed..Thus, the gamma 5 and gamma 7 subunits are the first G protein gamma subunits known that could participate in the regulation of widely distributed signal transduction pathways. ..
- Garden G, Hartlage Rübsamen M, Rubel E, Bothwell M. Protein masking of a ribosomal RNA epitope is an early event in afferent deprivation-induced neuronal death. Mol Cell Neurosci. 1995;6:293-310 pubmed..Immunoprecipitation experiments confirmed that the Y10B antibody recognizes a specific rRNA sequence without posttranscriptional modification. ..
- Li Q, Eckenrode S, Ruan Q, Wang C, Shi J, McIndoe R, et al. Rapid decrease of RNA level of a novel mouse mitochondria solute carrier protein (Mscp) gene at 4-5 weeks of age. Mamm Genome. 2001;12:830-6 pubmed..These results suggest that the Mscp gene is decreased during splenic lymphocyte maturation in these mice. ..
- Miyakawa S, Ferris J. Sequence- and regioselectivity in the montmorillonite-catalyzed synthesis of RNA. J Am Chem Soc. 2003;125:8202-8 pubmed..This research establishes that the montmorillonite catalyst limits the number of RNA oligomer isomers formed. The potential significance of these findings to the origins of life is discussed. ..
- Pierce J, Jegathesan J, Levant B, Winter M, McCarson K, Clancy R. Detection of dopamine receptor subtypes in the rat diaphragm. Heart Lung. 2007;36:58-63 pubmed..By identifying dopamine receptors in the diaphragm, we have a better understanding of the mechanisms by which dopamine treats and prevents diaphragm fatigue. ..
- Morse A, Carballo V, Baldwin D, Taylor C, McIntyre L. Comparison between NuGEN's WT-Ovation Pico and one-direct amplification systems. J Biomol Tech. 2010;21:141-7 pubmed..This is in marked contrast to the relatively few genes detected after amplification with the One-Direct amplification kit. ..
- Wang F, Zhang Z, Wang X, Li J, Zha L, Yuan C, et al. Genetic informational RNA is not required for recombinant prion infectivity. J Virol. 2012;86:1874-6 pubmed publisher..Our results show that genetic informational RNA is not required for recombinant prion infectivity. ..
- Liberman J, Salim M, Krucinska J, Wedekind J. Structure of a class II preQ1 riboswitch reveals ligand recognition by a new fold. Nat Chem Biol. 2013;9:353-5 pubmed publisher..The results account for translational control mediated by the preQ1-II riboswitch class and expand the known repertoire of ligand-binding modes used by regulatory RNAs. ..
- Lomax J, Eller C, Raines R. Comparative functional analysis of ribonuclease 1 homologs: molecular insights into evolving vertebrate physiology. Biochem J. 2017;474:2219-2233 pubmed publisher..These attributes have relevance for human health. Moreover, the functional diversification of the 10 RNase 1 homologs illuminates the regulation of extracellular RNA and other aspects of vertebrate evolution. ..
- Tan Z, Sharma G, Mathews D. Modeling RNA Secondary Structure with Sequence Comparison and Experimental Mapping Data. Biophys J. 2017;113:330-338 pubmed publisher..The updated version of TurboFold II is freely available as part of the RNAstructure software package. ..
- Brady J, Richmond R. Molecular analysis of evolutionary changes in the expression of Drosophila esterases. Proc Natl Acad Sci U S A. 1990;87:8217-21 pubmed..pseudoobscura, when isolated as a 4.5-kilobase restriction fragment, is not dosage compensated after integration into an autosome of D. melanogaster...
- Iverson L, Tanouye M, Lester H, Davidson N, Rudy B. A-type potassium channels expressed from Shaker locus cDNA. Proc Natl Acad Sci U S A. 1988;85:5723-7 pubmed..Alternative splicing of Sh gene transcripts may provide one mechanism for the generation of K+ channel diversity. ..
- Krieg P, Sakaguchi D, Kintner C. Primary structure and developmental expression of a large cytoplasmic domain form of Xenopus laevis neural cell adhesion molecule (NCAM). Nucleic Acids Res. 1989;17:10321-35 pubmed..No RNA encoding the small surface domain form of NCAM is detected during early development. These results indicate that the expression of NCAM sequences during early development of Xenopus differs from that described in other species. ..
- Schullery D, Ostrowski J, Denisenko O, Stempka L, Shnyreva M, Suzuki H, et al. Regulated interaction of protein kinase Cdelta with the heterogeneous nuclear ribonucleoprotein K protein. J Biol Chem. 1999;274:15101-9 pubmed..The regulated nature of the PKCdelta-K protein interaction may serve to meet cellular needs at sites of active transcription, RNA processing and translation in response to changing extracellular environment. ..
- Puranam R, Attardi G. The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the nuclear RNase P. Mol Cell Biol. 2001;21:548-61 pubmed..The available evidence indicates that the levels of mtRNase P detected in HeLa cells should be fully adequate to satisfy the mitochondrial tRNA synthesis requirements of these cells. ..
- Allawi H, Dong F, Ip H, Neri B, Lyamichev V. Mapping of RNA accessible sites by extension of random oligonucleotide libraries with reverse transcriptase. RNA. 2001;7:314-27 pubmed..Finally, we discuss the relationship between RNA extendible sites and RNA accessibility. ..
- Browner N, Dey N, Bloch K, Lincoln T. Regulation of cGMP-dependent protein kinase expression by soluble guanylyl cyclase in vascular smooth muscle cells. J Biol Chem. 2004;279:46631-6 pubmed..Likewise, the loss of PKG expression appears to increase sGC expression. These effects may be an adaptive mechanism allowing growth and survival of VSMC in vitro. ..
- Huang L, Hwang J, Sharma S, Hargittai M, Chen Y, Arnold J, et al. Hepatitis C virus nonstructural protein 5A (NS5A) is an RNA-binding protein. J Biol Chem. 2005;280:36417-28 pubmed publisher..The discovery that NS5A is an RNA-binding protein defines a new functional target for development of agents to treat HCV infection and a new structural class of RNA-binding proteins...
- Xu J, Knutson M, Carter C, Leeuwenburgh C. Iron accumulation with age, oxidative stress and functional decline. PLoS ONE. 2008;3:e2865 pubmed publisher..These findings strongly suggest that the age-related iron accumulation in muscle contributes to increased oxidative damage and sarcopenia, and that CR effectively attenuates these negative effects...
- Chadalavada D, Gratton E, Bevilacqua P. The human HDV-like CPEB3 ribozyme is intrinsically fast-reacting. Biochemistry. 2010;49:5321-30 pubmed publisher..It is therefore possible that cellular factors interact with regions upstream of the CPEB3 ribozyme to unmask its high intrinsic reactivity. ..
- Khanova E, Esakova O, Perederina A, Berezin I, Krasilnikov A. Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA-protein interactions. RNA. 2012;18:720-8 pubmed publisher..The results indicate locations of specific protein-binding sites in the RNA components of RNase P and RNase MRP and shed light on the structural organizations of these large ribonucleoprotein complexes. ..
- Wheeler T, Leger A, Pandey S, MacLeod A, Nakamori M, Cheng S, et al. Targeting nuclear RNA for in vivo correction of myotonic dystrophy. Nature. 2012;488:111-5 pubmed publisher..These results provide a general strategy to correct RNA gain-of-function effects and to modulate the expression of expanded repeats, lncRNAs and other transcripts with prolonged nuclear residence. ..