Genomes and Genes
Experts and Doctors on rna binding proteins in Belgium
Topic: rna binding proteins
- De Leeuw F, Zhang T, Wauquier C, Huez G, Kruys V, Gueydan C. The cold-inducible RNA-binding protein migrates from the nucleus to cytoplasmic stress granules by a methylation-dependent mechanism and acts as a translational repressor. Exp Cell Res. 2007;313:4130-44 pubmed..Altogether, our findings further reveal the diversity of mechanisms by which CIRP is regulated by environmental stresses and provide new insights into CIRP cytoplasmic function. ..
- Van Dyck F, Braem C, Chen Z, Declercq J, Deckers R, Kim B, et al. Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons. Cell Metab. 2007;6:406-13 pubmed..PlagL2 thus regulates important aspects of dietary lipid absorption, and the PlagL2(-/-) animal model has implications for the amelioration of obesity and the metabolic syndrome. ..
- Beullens M, Bollen M. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem. 2002;277:19855-60 pubmed..Our data suggest that NIPP1 can function independently as a splicing factor and a phosphatase regulator. ..
- Hensen K, Van Valckenborgh I, Kas K, Van de Ven W, Voz M. The tumorigenic diversity of the three PLAG family members is associated with different DNA binding capacities. Cancer Res. 2002;62:1510-7 pubmed..This strongly suggests that the oncogenic capacity of PLAG1 and PLAGL2 is mediated at least partly by activating the insulin-like growth factor-II mitogenic pathway. ..
- Kooy R, D Hooge R, Reyniers E, Bakker C, Nagels G, De Boulle K, et al. Transgenic mouse model for the fragile X syndrome. Am J Med Genet. 1996;64:241-5 pubmed..In the present study, we have extended our experiments, and conclude that the Fmr1 knockout mouse is a reliable transgenic model to study the fragile X syndrome. ..
- Verstappen G, van Grunsven L, Michiels C, Van de Putte T, Souopgui J, Van Damme J, et al. Atypical Mowat-Wilson patient confirms the importance of the novel association between ZFHX1B/SIP1 and NuRD corepressor complex. Hum Mol Genet. 2008;17:1175-83 pubmed publisher..This is the first study providing mechanistic insight into the aberrant function of a single domain of the multi-domain protein ZFHX1B/SIP1 in human disease. ..
- Forsyth R, De Boeck G, Bekaert S, De Meyer T, Taminiau A, Uyttendaele D, et al. Telomere biology in giant cell tumour of bone. J Pathol. 2008;214:555-63 pubmed publisher..Telomere maintenance could be considered an important key factor in the pathogenesis of GCTB...
- Jagiello I, Van Eynde A, Vulsteke V, Beullens M, Boudrez A, Keppens S, et al. Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1. J Cell Sci. 2000;113 Pt 21:3761-8 pubmed..This domain is also required for the nuclear retention of NIPP1 when active transport is blocked. Our data imply that the nuclear and subnuclear targeting of NIPP1 are controlled independently. ..
- Beullens M, Van Eynde A, Vulsteke V, Connor J, Shenolikar S, Stalmans W, et al. Molecular determinants of nuclear protein phosphatase-1 regulation by NIPP-1. J Biol Chem. 1999;274:14053-61 pubmed..Together, these data identified some of the key elements in the central domain of NIPP-1 that regulate PP-1 activity and suggested that the flanking sequences stabilize the association of NIPP-1 with PP-1C. ..
- Van Eynde A, Pérez Callejón E, Schoenmakers E, Jacquemin M, Stalmans W, Bollen M. Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1). Eur J Biochem. 1999;261:291-300 pubmed..While the NIPP-1alpha/beta/delta-transcripts were found to be present in various human tissues, the NIPP-1gamma transcript could only be detected in human transformed B-lymphocytes. ..
- Martinet W, De Meyer I, Cools N, Timmerman V, Bult H, Bosmans J, et al. Cell death-mediated cleavage of the attraction signal p43 in human atherosclerosis: implications for plaque destabilization. Arterioscler Thromb Vasc Biol. 2010;30:1415-22 pubmed publisher..Treatment of smooth muscle cells with p43 did not induce cell death. p43 is cleaved during apoptosis by calpains and released as a truncated protein that is harmless for the structure of the plaque. ..
- Timmermans J, Van Melderen L. Conditional essentiality of the csrA gene in Escherichia coli. J Bacteriol. 2009;191:1722-4 pubmed publisher..Deletion of the glgCAP operon in the csrA::cat mutant restored the ability to grow on LB and on synthetic medium containing glycolytic carbon sources, showing that growth inhibition is due to an excess of glycogen synthesis. ..
- Cleynen I, Brants J, Peeters K, Deckers R, Debiec Rychter M, Sciot R, et al. HMGA2 regulates transcription of the Imp2 gene via an intronic regulatory element in cooperation with nuclear factor-kappaB. Mol Cancer Res. 2007;5:363-72 pubmed..Finally, we provide evidence that there is a strong and statistically significant correlation between HMGA2 and IMP2 gene expression in human liposarcomas. ..
- Schepens B, Tinton S, Bruynooghe Y, Parthoens E, Haegman M, Beyaert R, et al. A role for hnRNP C1/C2 and Unr in internal initiation of translation during mitosis. EMBO J. 2007;26:158-69 pubmed..The observation that RNA interference-mediated knockdown of hnRNP C1/C2 and Unr, respectively, abrogates and retards mitosis points out that regulation of IRES-mediated translation by hnRNP C1/C2 and Unr might be important in mitosis. ..
- Tillemans V, Leponce I, Rausin G, Dispa L, Motte P. Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors. Plant Cell. 2006;18:3218-34 pubmed..Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments. ..
- Abdel Sater F, El Bakkoury M, Urrestarazu A, Vissers S, Andre B. Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Mol Cell Biol. 2004;24:9771-85 pubmed..Upon detection of external amino acids, Ssy1-Ptr3 likely allows-in a manner dependent on SCF(Grr1)-the Ssy5 endoprotease to gain access to and to cleave Stp1, this requiring prior phosphorylation of Stp1 by casein kinase I. ..
- Kharraz Y, Salmand P, Camus A, Auriol J, Gueydan C, Kruys V, et al. Impaired embryonic development in mice overexpressing the RNA-binding protein TIAR. PLoS ONE. 2010;5:e11352 pubmed publisher..This study reports the importance of the tightly balanced expression of the RNA-binding protein TIAR for normal embryonic development, thereby emphasizing the role of post-transcriptional regulations in early embryonic programming. ..
- Bonif M, Meuwis M, Close P, Benoit V, Heyninck K, Chapelle J, et al. TNFalpha- and IKKbeta-mediated TANK/I-TRAF phosphorylation: implications for interaction with NEMO/IKKgamma and NF-kappaB activation. Biochem J. 2006;394:593-603 pubmed
- Beullens M, Vulsteke V, Van Eynde A, Jagiello I, Stalmans W, Bollen M. The C-terminus of NIPP1 (nuclear inhibitor of protein phosphatase-1) contains a novel binding site for protein phosphatase-1 that is controlled by tyrosine phosphorylation and RNA binding. Biochem J. 2000;352 Pt 3:651-8 pubmed..Our data are in agreement with a shared-site model for the interaction of PP1(C) with its regulatory subunits. ..
- Marchal C, Ismaili N, Pays E. A ribosomal S12-like gene of Trypanosoma brucei. Mol Biochem Parasitol. 1993;57:331-4 pubmed
- Ismaili N, Perez Morga D, Walsh P, Mayeda A, Pays A, Tebabi P, et al. Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins. Mol Biochem Parasitol. 1999;102:103-15 pubmed..These and other observations suggest that TSR1 may be involved in trans-splicing in T. brucei. ..
- Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, et al. NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry. J Biol Chem. 2000;275:25411-7 pubmed..We suggest that NIPP1 either targets CDC5L or an associated protein for dephosphorylation by PP1 or serves as an anchor for both PP1 and CDC5L. ..
- Zhang T, Delestienne N, Huez G, Kruys V, Gueydan C. Identification of the sequence determinants mediating the nucleo-cytoplasmic shuttling of TIAR and TIA-1 RNA-binding proteins. J Cell Sci. 2005;118:5453-63 pubmed..This study demonstrates the importance of TIAR and TIA-1 RNA-binding domains for their subcellular localization and provides the first evidence for distinct functions of TIAR and TIA-1 RRMs. ..
- Boudrez A, Beullens M, Waelkens E, Stalmans W, Bollen M. Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1. J Biol Chem. 2002;277:31834-41 pubmed..We discuss how the interaction between NIPP1 and SAP155 could contribute to spliceosome (dis)assembly and the catalytic steps of splicing. ..
- Gueydan C, Wauquier C, De Mees C, Huez G, Kruys V. Identification of ribosomal proteins specific to higher eukaryotic organisms. J Biol Chem. 2002;277:45034-40 pubmed..In contrast, no homologous protein is found in lower eukaryotes such as yeast and Caenorhabditis elegans. These observations indicate that PS1D is the first eukaryotic ribosomal protein that is specific to higher eukaryotes. ..
- Lamkanfi M, Kalai M, Saelens X, Declercq W, Vandenabeele P. Caspase-1 activates nuclear factor of the kappa-enhancer in B cells independently of its enzymatic activity. J Biol Chem. 2004;279:24785-93 pubmed..These data demonstrate that caspase-1 contributes to inflammation by two distinct pathways: proteolysis of pro-IL-1beta, and RIP2-dependent activation of NF-kappaB and p38 MAPK mediated by the caspase recruitment domain. ..
- Strumane K, Bonnomet A, Stove C, Vandenbroucke R, Nawrocki Raby B, Bruyneel E, et al. E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion. Cancer Res. 2006;66:10007-15 pubmed..Targeting hNanos1 might be a promising strategy in the treatment of E-cadherin-negative tumors in particular. ..
- Cornelis S, Tinton S, Schepens B, Bruynooghe Y, Beyaert R. UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein. Nucleic Acids Res. 2005;33:3095-108 pubmed..In conclusion, our results demonstrate that translation of the ITAF Unr can itself be regulated by an IRES that is downregulated by PTB. ..
- Jagiello I, Beullens M, Vulsteke V, Wera S, Sohlberg B, Stalmans W, et al. NIPP-1, a nuclear inhibitory subunit of protein phosphatase-1, has RNA-binding properties. J Biol Chem. 1997;272:22067-71 pubmed..The catalytic subunit of protein phosphatase-1 did not bind to poly(U)-Sepharose, but it bound very tightly after complexation with NIPP-1. These data are in agreement with a function of NIPP-1 in targeting protein phosphatase-1 to RNA. ..
- Terryn N, Neyt P, De Clercq R, De Keyser A, Van den Daele H, Ardiles W, et al. Sequence analysis of a 24-kb contiguous genomic region at the Arabidopsis thaliana PFL locus on chromosome 1. FEBS Lett. 1997;416:156-60 pubmed..Intriguingly, the gene encoding the PAB-3 protein, which is very specifically expressed, is flanked by putative matrix attachment regions. ..
- Vulsteke V, Beullens M, Waelkens E, Stalmans W, Bollen M. Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1). J Biol Chem. 1997;272:32972-8 pubmed
- Kas K, Voz M, Hensen K, Meyen E, Van de Ven W. Transcriptional activation capacity of the novel PLAG family of zinc finger proteins. J Biol Chem. 1998;273:23026-32 pubmed..This effect is less profound in epithelial cells. These data suggest that the activation in pleomorphic adenomas of PLAG1 most likely results in uncontrolled activation of downstream target genes. ..
- Rausin G, Tillemans V, Stankovic N, Hanikenne M, Motte P. Dynamic nucleocytoplasmic shuttling of an Arabidopsis SR splicing factor: role of the RNA-binding domains. Plant Physiol. 2010;153:273-84 pubmed publisher..Moreover, our data suggest that CRM1 is a putative export receptor for mRNPs in plants. ..
- Tillemans V, Dispa L, Remacle C, Collinge M, Motte P. Functional distribution and dynamics of Arabidopsis SR splicing factors in living plant cells. Plant J. 2005;41:567-82 pubmed..SR proteins also localized within small foci at anaphase. These results demonstrate interesting related features as well as potentially important differences between plant and animal SR proteins. ..
- Jin Q, Van Eynde A, Beullens M, Roy N, Thiel G, Stalmans W, et al. The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor. J Biol Chem. 2003;278:30677-85 pubmed..Also, the histone deacetylase 2 was present in a complex with NIPP1. Our data are in accordance with a role for NIPP1 as a DNA-targeting protein for EED and associated chromatin-modifying enzymes. ..
- De Weer A, Speleman F, Cauwelier B, Van Roy N, Yigit N, Verhasselt B, et al. EVI1 overexpression in t(3;17) positive myeloid malignancies results from juxtaposition of EVI1 to the MSI2 locus at 17q22. Haematologica. 2008;93:1903-7 pubmed publisher..In keeping with findings in other recurrent 3q26 rearrangements, overexpression of the EVI1 gene appears to be the major contributor to leukemogenesis in patients with a t(3;17). ..
- Souopgui J, Rust B, Vanhomwegen J, Heasman J, Henningfeld K, Bellefroid E, et al. The RNA-binding protein XSeb4R: a positive regulator of VegT mRNA stability and translation that is required for germ layer formation in Xenopus. Genes Dev. 2008;22:2347-52 pubmed publisher..These observations unravel a novel mode of VegT regulation at the post-transcriptional level that is essential for germ layer formation in Xenopus. ..
- Brants J, Ayoubi T, Chada K, Marchal K, Van de Ven W, Petit M. Differential regulation of the insulin-like growth factor II mRNA-binding protein genes by architectural transcription factor HMGA2. FEBS Lett. 2004;569:277-83 pubmed..Our results clearly indicate that HMGA2 differentially regulates expression of IMP family members during embryogenesis. ..
- Jun L, Frints S, Duhamel H, Herold A, Abad Rodrigues J, Dotti C, et al. NXF5, a novel member of the nuclear RNA export factor family, is lost in a male patient with a syndromic form of mental retardation. Curr Biol. 2001;11:1381-91 pubmed..Although we did not find direct evidence for the involvement of NXF5 in MR, the gene could be involved in development, possibly through a process in mRNA metabolism in neurons. ..
- Vulsteke V, Beullens M, Boudrez A, Keppens S, Van Eynde A, Rider M, et al. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1. J Biol Chem. 2004;279:8642-7 pubmed..Our data suggest that MELK has a role in the cell cycle-regulated control of pre-mRNA splicing. ..
- Zhang T, Kruys V, Huez G, Gueydan C. AU-rich element-mediated translational control: complexity and multiple activities of trans-activating factors. Biochem Soc Trans. 2002;30:952-8 pubmed..These findings also suggest that ARE-binding proteins interact with RNA as multimeric complexes, which might define their function and their sequence specificity. ..