Experts and Doctors on ribonucleoproteins in Germany


Locale: Germany
Topic: ribonucleoproteins

Top Publications

  1. Wahl M, Will C, Luhrmann R. The spliceosome: design principles of a dynamic RNP machine. Cell. 2009;136:701-18 pubmed publisher
  2. Raiz J, Damert A, Chira S, Held U, Klawitter S, Hamdorf M, et al. The non-autonomous retrotransposon SVA is trans-mobilized by the human LINE-1 protein machinery. Nucleic Acids Res. 2012;40:1666-83 pubmed publisher
    ..SVA de novo insertions were predominantly full-length, occurred preferentially in G+C-rich regions, and displayed all features of L1-mediated retrotransposition which are also observed in preexisting genomic SVA insertions. ..
  3. Kruse C, Jaedicke A, Beaudouin J, Bohl F, Ferring D, Guttler T, et al. Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p. J Cell Biol. 2002;159:971-82 pubmed
    ..Loss of She2p can be complemented by direct targeting of a heterologous lacZ mRNA to a complex of Myo4p and its associated adaptor She3p, suggesting that She2p's function in Myo4p targeting is to link an mRNA to the motor complex. ..
  4. Klingen Y, Conzelmann K, Finke S. Double-labeled rabies virus: live tracking of enveloped virus transport. J Virol. 2008;82:237-45 pubmed
    ..This indicates that the typical retrograde axonal transport of RV to the central nervous system involves neuronal transport vesicles in which complete enveloped RV particles are carried as a cargo. ..
  5. Muller D, Rehbein M, Baumeister H, Richter D. Molecular characterization of a novel rat protein structurally related to poly(A) binding proteins and the 70K protein of the U1 small nuclear ribonucleoprotein particle (snRNP). Nucleic Acids Res. 1992;20:1471-5 pubmed
    ..Ontogenic studies reveal that the expression of the 100 kDa protein-encoding gene and sexual maturation are correlated, being barely detectable during early post-natal life but reaching maximal levels around the first month after birth. ..
  6. Horke S, Reumann K, Rang A, Heise T. Molecular characterization of the human La protein.hepatitis B virus RNA.B interaction in vitro. J Biol Chem. 2002;277:34949-58 pubmed
    ..Hence, these binding sites might represent targets for novel antiviral strategies based on the disruption of the human La.HBV RNA interaction, thereby leading to HBV RNA degradation. ..
  7. Schmidt U, Richter K, Berger A, Lichter P. In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains. J Cell Biol. 2006;172:373-81 pubmed
    ..These results suggest that a fraction of RNA, which remains in the nucleus for several hours despite its association with splicing and export proteins, accumulates in speckles because of an ATP-dependent mechanism...
  8. Du T, Jellbauer S, Müller M, Schmid M, Niessing D, Jansen R. Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA. EMBO Rep. 2008;9:781-7 pubmed publisher
    ..Our results indicate that the nucleolar transit of RNA-binding proteins such as She2 is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins. ..
  9. Kullmann M, Göpfert U, Siewe B, Hengst L. ELAV/Hu proteins inhibit p27 translation via an IRES element in the p27 5'UTR. Genes Dev. 2002;16:3087-99 pubmed
    ..Consistent with an inhibitory role of Hu proteins in p27 translation, siRNA mediated knockdown of HuR induced endogenous p27 protein levels as well as IRES-mediated reporter translation and leads to cell cycle arrest in G1. ..

More Information


  1. Singh K, Erkelenz S, Rattay S, Dehof A, Hildebrandt A, Schulze Osthoff K, et al. Human SAP18 mediates assembly of a splicing regulatory multiprotein complex via its ubiquitin-like fold. RNA. 2010;16:2442-54 pubmed publisher
    ..Our results therefore suggest a novel function of SAP18 in splicing regulation. ..
  2. Mackereth C, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, et al. Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature. 2011;475:408-11 pubmed publisher
    ..Similar mechanisms of cooperative multi-domain conformational selection may operate more generally in the recognition of degenerate nucleotide or amino acid motifs by multi-domain proteins. ..
  3. Fischer U, Sumpter V, Sekine M, Satoh T, Luhrmann R. Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap. EMBO J. 1993;12:573-83 pubmed
    ..We propose that the RNA structure of a given snRNP particle determines at least in part whether the particle's m3G cap is required for nuclear transport or can be dispensed with. ..
  4. Eggert M, Michel J, Schneider S, Bornfleth H, Baniahmad A, Fackelmayer F, et al. The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U. J Biol Chem. 1997;272:28471-8 pubmed
    ..A possible functional role for this GR binding-protein in addition to its binding to the nuclear matrix, to RNA, and to scaffold attachment regions is discussed. ..
  5. Kremerskothen J, Nettermann M, Op De Bekke A, Bachmann M, Brosius J. Identification of human autoantigen La/SS-B as BC1/BC200 RNA-binding protein. DNA Cell Biol. 1998;17:751-9 pubmed
    ..The fact that La binds both BC1 and BC200 RNAs further supports the notion that the RNAs are functional analogs despite the fact that they arose from two separate retroposition events in two different mammalian lineages. ..
  6. Spiegelberg L, Wahl Jensen V, Kolesnikova L, Feldmann H, Becker S, Hoenen T. Genus-specific recruitment of filovirus ribonucleoprotein complexes into budding particles. J Gen Virol. 2011;92:2900-5 pubmed publisher
    ..These results not only further our understanding of VP40 interactions, but also suggest that special care is required when using trVLP or VLP systems to model virus morphogenesis. ..
  7. Koebernick K, Loeber J, Arthur P, Tarbashevich K, Pieler T. Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma. Proc Natl Acad Sci U S A. 2010;107:16148-53 pubmed publisher
    ..ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance...
  8. Boehm J, Orth T, Van Nguyen P, Söling H. Systemic lupus erythematosus is associated with increased auto-antibody titers against calreticulin and grp94, but calreticulin is not the Ro/SS-A antigen. Eur J Clin Invest. 1994;24:248-57 pubmed
  9. Day F, Thompson D, Helgason H, Chasman D, Finucane H, Sulem P, et al. Genomic analyses identify hundreds of variants associated with age at menarche and support a role for puberty timing in cancer risk. Nat Genet. 2017;49:834-841 pubmed publisher
    ..In aggregate, our findings highlight the complexity of the genetic regulation of puberty timing and support causal links with cancer susceptibility. ..
  10. Senger B, Simos G, Bischoff F, Podtelejnikov A, Mann M, Hurt E. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p. EMBO J. 1998;17:2196-207 pubmed
    ..This suggests that Npl3p follows a distinct nuclear import pathway and that intranuclear release from its specific import receptor Mtr10p requires the cooperative action of both Ran-GTP and newly synthesized mRNA. ..
  11. Höck J, Weinmann L, Ender C, Rüdel S, Kremmer E, Raabe M, et al. Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. EMBO Rep. 2007;8:1052-60 pubmed
    ..Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation. ..
  12. Chari A, Paknia E, Fischer U. The role of RNP biogenesis in spinal muscular atrophy. Curr Opin Cell Biol. 2009;21:387-93 pubmed publisher
    ..The detailed biochemical investigation of SMN under normal conditions and in SMA has provided clues how mutations in factors with general functions elicit tissue-specific phenotypes. ..
  13. Nagel J, Gross B, Meggendorfer M, Preiss C, Grez M, Brack Werner R, et al. Stably integrated and expressed retroviral sequences can influence nuclear location and chromatin condensation of the integration locus. Chromosoma. 2012;121:353-67 pubmed publisher
    ..We thus present an example where a few kb of exogenous DNA are sufficient to significantly alter the large-scale chromatin organization of an endogenous locus. ..
  14. Licht K, Medenbach J, Luhrmann R, Kambach C, Bindereif A. 3'-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins. RNA. 2008;14:1532-8 pubmed publisher
  15. Madl T, Felli I, Bertini I, Sattler M. Structural analysis of protein interfaces from 13C direct-detected paramagnetic relaxation enhancements. J Am Chem Soc. 2010;132:7285-7 pubmed publisher
    ..The (13)C PREs provide long-range distance restraints to map binding interfaces in proteins and protein complexes and are especially useful for studies of high-molecular weight perdeuterated molecules. ..
  16. Setny P, Zacharias M. A coarse-grained force field for Protein-RNA docking. Nucleic Acids Res. 2011;39:9118-29 pubmed publisher
    ..The coarse-grained representation allows for fast and efficient systematic docking search without any prior knowledge about complex geometry. ..
  17. Niedner A, Müller M, Moorthy B, Jansen R, Niessing D. Role of Loc1p in assembly and reorganization of nuclear ASH1 messenger ribonucleoprotein particles in yeast. Proc Natl Acad Sci U S A. 2013;110:E5049-58 pubmed publisher
    ..Together these findings suggest an ordered process of nuclear assembly and reorganization for the maturation of localizing ASH1 mRNPs. ..
  18. Zhou F, Liu Y, Rohde C, Pauli C, Gerloff D, Köhn M, et al. AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia. Nat Cell Biol. 2017;19:844-855 pubmed publisher
    ..Collectively, these findings indicate that induction of C/D box snoRNA/RNP function constitutes an important pathway in leukaemogenesis. ..
  19. Fischer T, Rodriguez Navarro S, Pereira G, Rácz A, Schiebel E, Hurt E. Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat Cell Biol. 2004;6:840-8 pubmed
    ..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery. ..
  20. Kubitscheck U, GRUNWALD D, Hoekstra A, Rohleder D, Kues T, Siebrasse J, et al. Nuclear transport of single molecules: dwell times at the nuclear pore complex. J Cell Biol. 2005;168:233-43 pubmed
    ..Together with the known transport rates, our data suggest that nucleocytoplasmic transport occurs via multiple parallel pathways within single NPCs. ..
  21. Zhang Y, Madl T, Bagdiul I, Kern T, Kang H, Zou P, et al. Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 3'-splice site recognition. Nucleic Acids Res. 2013;41:1343-54 pubmed publisher
    ..Our results indicate that the helix-hairpin domain of SF1 is required for cooperative 3'-splice site recognition presumably by stabilizing a unique quaternary arrangement of the SF1-U2AF65-RNA complex. ..
  22. Frohn A, Eberl H, Stöhr J, Glasmacher E, Rüdel S, Heissmeyer V, et al. Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells. Mol Cell Proteomics. 2012;11:1442-56 pubmed publisher
    ..In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins. ..
  23. Batisse J, Batisse C, Budd A, Bottcher B, Hurt E. Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem. 2009;284:34911-7 pubmed publisher
    ..Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure. ..
  24. Murachelli A, Ebert J, Basquin C, Le Hir H, Conti E. The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex. Nat Struct Mol Biol. 2012;19:378-86 pubmed publisher
    ..Unexpectedly, our structural analysis identified an Acinus-like motif in Pinin, another EJC-associated splicing factor. We show that Pinin physically interacts with RNPS1 and SAP18, forming an alternative ternary complex, PSAP. ..
  25. Muddashetty R, Khanam T, Kondrashov A, Bundman M, Iacoangeli A, Kremerskothen J, et al. Poly(A)-binding protein is associated with neuronal BC1 and BC200 ribonucleoprotein particles. J Mol Biol. 2002;321:433-45 pubmed
    ..These findings lend support to the hypothesis that the BC1 and BC200 RNPs are involved in protein translation in neuronal dendrites. ..
  26. Engemann H, Heinzel V, Page G, Preuss U, Scheidtmann K. DAP-like kinase interacts with the rat homolog of Schizosaccharomyces pombe CDC5 protein, a factor involved in pre-mRNA splicing and required for G2/M phase transition. Nucleic Acids Res. 2002;30:1408-17 pubmed
    ..Together with previous data, these results suggest that Dlk might play a role in coordinating specific transcription and splicing events. ..
  27. Fackelmayer F, Richter A. hnRNP-U/SAF-A is encoded by two differentially polyadenylated mRNAs in human cells. Biochim Biophys Acta. 1994;1217:232-4 pubmed
    ..9 kb and 3.1 kb, respectively, exist in human cells. Using selective cDNA fragments as probes we show that the shorter mRNA lacks 800 nucleotides of the untranslated 3' end. ..
  28. Fackelmayer F, Dahm K, Renz A, Ramsperger U, Richter A. Nucleic-acid-binding properties of hnRNP-U/SAF-A, a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro. Eur J Biochem. 1994;221:749-57 pubmed
    ..We conclude that hnRNP-U/SAF-A thus may have functions in the organisation of chromosomal DNA in addition to its suggested role in hnRNA metabolism. ..
  29. Willeke F, Ridder R, Mechtersheimer G, Schwarzbach M, Duwe A, Weitz J, et al. Analysis of FUS-CHOP fusion transcripts in different types of soft tissue liposarcoma and their diagnostic implications. Clin Cancer Res. 1998;4:1779-84 pubmed
    ..Furthermore, using FUS-CHOP fusion transcripts as targets in reverse transcription-PCR assays, we detected disseminated tumor cells in peripheral blood or bone marrow in 3 of 5 patients undergoing surgery for soft tissue liposarcoma. ..
  30. Rossoll W, Kröning A, Ohndorf U, Steegborn C, Jablonka S, Sendtner M. Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons?. Hum Mol Genet. 2002;11:93-105 pubmed
    ..Interestingly, hnRNP-R is predominantly located in axons of motor neurons and co-localizes with Smn in this cellular compartment. Thus, this finding could provide a key to understand a motor neuron-specific Smn function in SMA. ..
  31. Müller Taubenberger A, Lupas A, Li H, Ecke M, Simmeth E, Gerisch G. Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis. EMBO J. 2001;20:6772-82 pubmed
  32. Fischer U, Pollard V, Luhrmann R, Teufel M, Michael M, Dreyfuss G, et al. Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycle. Nucleic Acids Res. 1999;27:4128-34 pubmed
    ..Finally, we demonstrate that Rev/RRE-activated RNA export is, like other nuclear export pathways, linked to the Ran-GTPase cycle. ..
  33. Kohler M, Speck C, Christiansen M, Bischoff F, Prehn S, Haller H, et al. Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import. Mol Cell Biol. 1999;19:7782-91 pubmed
    ..However, strikingly different substrate preferences of the various importin alpha proteins were revealed when two substrates were offered simultaneously. ..
  34. Kzhyshkowska J, Schutt H, Liss M, Kremmer E, Stauber R, Wolf H, et al. Heterogeneous nuclear ribonucleoprotein E1B-AP5 is methylated in its Arg-Gly-Gly (RGG) box and interacts with human arginine methyltransferase HRMT1L1. Biochem J. 2001;358:305-14 pubmed
    ..The Src homology 3 (SH3) domain of HRMT1L1 was essential for its interaction with E1B-AP5 in vivo. We suggest that HRMT1L1 is responsible for specific E1B-AP5 methylation in vivo. ..
  35. Heinrichs V, Hackl W, Luhrmann R. Direct binding of small nuclear ribonucleoprotein G to the Sm site of small nuclear RNA. Ultraviolet light cross-linking of protein G to the AAU stretch within the Sm site (AAUUUGUGG) of U1 small nuclear ribonucleoprotein reconstituted in vitro. J Mol Biol. 1992;227:15-28 pubmed
    ..These results suggest that the snRNP protein G may be involved in the direct recognition of the Sm site. ..
  36. Duning K, Buck F, Barnekow A, Kremerskothen J. SYNCRIP, a component of dendritically localized mRNPs, binds to the translation regulator BC200 RNA. J Neurochem. 2008;105:351-9 pubmed
    ..Thus, SYNCRIP may recruit BC200 RNA into mRNA transport complexes involved in the regulation of localized translation in dendrites. ..
  37. Cordes S, Kusov Y, Heise T, Gauss Muller V. La autoantigen suppresses IRES-dependent translation of the hepatitis A virus. Biochem Biophys Res Commun. 2008;368:1014-9 pubmed publisher
    ..The La-mediated suppression of HAV translation and stimulation of poliovirus translation implies unexpected mechanistic differences between viral IRES elements. ..
  38. Bunse A, Nickelsen J, Kuck U. Intron-specific RNA binding proteins in the chloroplast of the green alga Chlamydomonas reinhardtii. Biochim Biophys Acta. 2001;1519:46-54 pubmed
    ..We propose that we have biochemically characterized 'general splicing factors', which enable the successful splicing even of mitochondrial introns in chloroplasts. ..
  39. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing. ..
  40. Eulalio A, Huntzinger E, Nishihara T, Rehwinkel J, Fauser M, Izaurralde E. Deadenylation is a widespread effect of miRNA regulation. RNA. 2009;15:21-32 pubmed publisher
    ..These results indicate that miRNAs promote mRNA decay by altering mRNP composition and/or conformation, rather than by directly interfering with the binding and function of ribosomal subunits. ..
  41. Dolnik O, Kolesnikova L, Stevermann L, Becker S. Tsg101 is recruited by a late domain of the nucleocapsid protein to support budding of Marburg virus-like particles. J Virol. 2010;84:7847-56 pubmed publisher
    ..Taken together, these data indicate that NP enhances budding of VLPs by recruiting Tsg101 to the VP40-positive budding site through a PSAP late-domain motif. ..
  42. Knittler M, Gülow K, Seelig A, Howard J. MHC class I molecules compete in the endoplasmic reticulum for access to transporter associated with antigen processing. J Immunol. 1998;161:5967-77 pubmed
  43. Fetzer S, Lauber J, Will C, Luhrmann R. The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase. RNA. 1997;3:344-55 pubmed
    ..Thus, the tri-snRNP-specific 27K protein could potentially be involved in SR protein-mediated protein/protein interactions and, additionally, its phosphorylation state could modulate pre-mRNA splicing. ..
  44. Dresselhaus T, Hagel C, Lorz H, Kranz E. Isolation of a full-length cDNA encoding calreticulin from a PCR library of in vitro zygotes of maize. Plant Mol Biol. 1996;31:23-34 pubmed
    ..The described calreticulin gene represents to our knowledge the first cDNA clone isolated from a RT/PCR cDNA library originating from only a few plant cells and is the first gene isolated from zygotes of higher plants. ..
  45. Aumiller V, Graebsch A, Kremmer E, Niessing D, Forstemann K. Drosophila Pur-? binds to trinucleotide-repeat containing cellular RNAs and translocates to the early oocyte. RNA Biol. 2012;9:633-43 pubmed publisher
    ..Related sequences, such as r(CAG) 4 and the consensus sequence of the opa-repeat r(CAG) 3CAA, can also associate with Pur-? in vitro and in vivo. The mRNA target spectrum of Pur-? may therefore be larger than previously anticipated. ..
  46. Preußner M, Wilhelmi I, Schultz A, Finkernagel F, Michel M, Moroy T, et al. Rhythmic U2af26 alternative splicing controls PERIOD1 stability and the circadian clock in mice. Mol Cell. 2014;54:651-62 pubmed publisher
    ..These data suggest light-induced U2af26 alternative splicing to be a buffering mechanism that limits PERIOD1 induction, thus stabilizing the circadian clock against abnormal changes in light:dark conditions. ..
  47. Eulalio A, Behm Ansmant I, Schweizer D, Izaurralde E. P-body formation is a consequence, not the cause, of RNA-mediated gene silencing. Mol Cell Biol. 2007;27:3970-81 pubmed
    ..Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity...
  48. Selenko P, Gregorovic G, Sprangers R, Stier G, Rhani Z, Kramer A, et al. Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Mol Cell. 2003;11:965-76 pubmed
    ..This molecular recognition establishes a paradigm for protein binding by a subfamily of noncanonical RRMs. ..
  49. Feltens R, Gossringer M, Willkomm D, Urlaub H, Hartmann R. An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains. Proc Natl Acad Sci U S A. 2003;100:5724-9 pubmed publisher
    ..Taken together, these data reveal a mode of gene expression that is to our knowledge unprecedented in bacteria...
  50. Huttelmaier S, Illenberger S, Grosheva I, Rudiger M, Singer R, Jockusch B. Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins. J Cell Biol. 2001;155:775-86 pubmed
    ..During muscle differentiation, raver1 migrates from the nucleus to the costamere. We propose that raver1 may coordinate RNA processing and targeting as required for microfilament anchoring in specific adhesion sites. ..
  51. Seidenbecher C, Landwehr M, Smalla K, Kreutz M, Dieterich D, Zuschratter W, et al. Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins. J Mol Biol. 2004;336:957-70 pubmed
    ..These findings provide molecular evidence that NCS can transduce signals to a specific target interaction irrespective of Ca(2+)-concentrations and CaM-levels. ..
  52. Ehlers I, Horke S, Reumann K, Rang A, Grosse F, Will H, et al. Functional characterization of the interaction between human La and hepatitis B virus RNA. J Biol Chem. 2004;279:43437-47 pubmed
  53. Meins M, Schlickum S, Wilhelm C, Missbach J, Yadav S, Glaser B, et al. Identification and characterization of murine Brunol4, a new member of the elav/bruno family. Cytogenet Genome Res. 2002;97:254-60 pubmed
    ..We predict a role of Brunol4 and the respective human homologue in differentiation and maintenance of neuronal structures. ..
  54. Behrens S, Luhrmann R. Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells. Genes Dev. 1991;5:1439-52 pubmed
    ..U5] is phosphorylated only in the latter. The function of this phosphorylation is unclear thus far; it may be involved in the activation of [U4/U6.U5] in the spliceosome. ..
  55. Wilk H, Schaefer K, Agris P, Boak A, Kovacs S. U1 SnRNP association with HnRNP involves an initial non-specific splice-site independent interaction of U1 SnRNP protein with HnRNA. Mol Cell Biochem. 1991;106:55-66 pubmed
    ..This complex may then be further stabilized by intron-specific interactions and hnRNP proteins, as well as by other snRNPs. ..
  56. Schubert T, Pusch M, Diermeier S, Benes V, Kremmer E, Imhof A, et al. Df31 protein and snoRNAs maintain accessible higher-order structures of chromatin. Mol Cell. 2012;48:434-44 pubmed publisher
    ..Analysis of caRNAs in human cells also reveals a strong enrichment of snoRNAs, implying a conserved role for these molecules in higher-order structures of chromatin. ..
  57. Macchi P, Kroening S, Palacios I, Baldassa S, Grunewald B, Ambrosino C, et al. Barentsz, a new component of the Staufen-containing ribonucleoprotein particles in mammalian cells, interacts with Staufen in an RNA-dependent manner. J Neurosci. 2003;23:5778-88 pubmed
    ..Together, our data suggest that the mRNA transport machinery is conserved during evolution, and that mammalian Btz is an additional component of the dendritic RNPs in hippocampal neurons. ..
  58. Huwiler A, Akool E, Aschrafi A, Hamada F, Pfeilschifter J, Eberhardt W. ATP potentiates interleukin-1 beta-induced MMP-9 expression in mesangial cells via recruitment of the ELAV protein HuR. J Biol Chem. 2003;278:51758-69 pubmed
    ..Together, our results indicate that the amplification of MMP-9 expression by extracellular ATP is triggered through mechanisms that likely involve a HuR-dependent rise in MMP-9 mRNA stability. ..
  59. Orth T, Dorner T, Meyer zum Buschenfelde K, Mayet W. Complete congenital heart block is associated with increased autoantibody titers against calreticulin. Eur J Clin Invest. 1996;26:205-15 pubmed
    ..The new finding of IgM autoantibodies and the observed differences in antibody response in infants and mothers support the hypothesis of a fetally mediated and passively acquired autoimmune disease. ..
  60. Horke S, Reumann K, Schweizer M, Will H, Heise T. Nuclear trafficking of La protein depends on a newly identified nucleolar localization signal and the ability to bind RNA. J Biol Chem. 2004;279:26563-70 pubmed
    ..Directed trafficking of hLa is fully consistent with its function in the maturation of precursor RNAs located in different subnuclear compartments. ..
  61. Beil B, Screaton G, Stamm S. Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. DNA Cell Biol. 1997;16:679-90 pubmed
    ..The ratio of these two isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in regulation of htra2-beta activity. ..
  62. Nayler O, Cap C, Stamm S. Human transformer-2-beta gene (SFRS10): complete nucleotide sequence, chromosomal localization, and generation of a tissue-specific isoform. Genomics. 1998;53:191-202 pubmed
    ..Finally, we show that htra2-beta3 is expressed in the nucleus and interacts with a subset of SR proteins in a yeast two-hybrid system and in vivo. ..
  63. Walther T, Fornerod M, Pickersgill H, Goldberg M, Allen T, Mattaj I. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. EMBO J. 2001;20:5703-14 pubmed
    ..Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events. ..
  64. Heide C, Pfeiffer T, Nolan J, Hartmann R. Guanosine 2-NH2 groups of Escherichia coli RNase P RNA involved in intramolecular tertiary contacts and direct interactions with tRNA. RNA. 1999;5:102-16 pubmed
    ..coli RNase P RNA) is also supported by mutational analysis. For the mutant RNase P RNA carrying a G94:C104 double mutation, an additional G316-to-A mutation resulted in a restoration of binding affinity for mature and precursor tRNA. ..
  65. Brendel C, Rehbein M, Kreienkamp H, Buck F, Richter D, Kindler S. Characterization of Staufen 1 ribonucleoprotein complexes. Biochem J. 2004;384:239-46 pubmed
  66. Ulfig N, Briese V. Immunolabelling of spliceosomes in sections and cultured astrocytes of human fetal brain tissue. Brain Dev. 1999;21:51-8 pubmed
    ..The in vitro finding indicates that anti-SC35 may well be used as a tool to study possible alterations of the speckles after, for instance, application of growth factors. ..
  67. Perlewitz A, Nafz B, Skalweit A, Fähling M, Persson P, Thiele B. Aldosterone and vasopressin affect {alpha}- and {gamma}-ENaC mRNA translation. Nucleic Acids Res. 2010;38:5746-60 pubmed publisher
    ..Immunoprecipitation and UV-crosslinking analysis of ?-ENaC-mRNA/HuR complexes document the significance of ?-ENaC-mRNA-3'-UTR/HuR interaction for hormonal control of ENaC synthesis...
  68. Andrei M, Ingelfinger D, Heintzmann R, Achsel T, Rivera Pomar R, Luhrmann R. A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies. RNA. 2005;11:717-27 pubmed
    ..These data support a model in which mRNPs undergo several successive steps of remodeling and/or 3' trimming until their composition or structural organization promotes their accumulation in P bodies. ..
  69. Kipp M, Schwab B, Przybylski M, Nicotera P, Fackelmayer F. Apoptotic cleavage of scaffold attachment factor A (SAF-A) by caspase-3 occurs at a noncanonical cleavage site. J Biol Chem. 2000;275:5031-6 pubmed
    ..A point mutation, D100A, abrogates cleavage by recombinant caspase-3 in vitro and during apoptosis in vivo, confirming SALD as a novel caspase-3 cleavage site. ..
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