Genomes and Genes
Experts and Doctors on recombinant fusion proteins in United Kingdom
Locale: United Kingdom
Topic: recombinant fusion proteins
Publications227 found, 100 shown here
- Carter S, Vousden K. p53-Ubl fusions as models of ubiquitination, sumoylation and neddylation of p53. Cell Cycle. 2008;7:2519-28 pubmed..However, in our experiments we find that the export of p53 from the nucleus is not sufficient to activate its cytoplasmic apoptotic function which may depend on the ability to deubiquitinate cytoplasmic p53. ..
- Loureiro S, Ren J, Phapugrangkul P, Colaco C, Bailey C, Shelton H, et al. Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines. J Virol. 2011;85:3010-4 pubmed publisher..HuFc-tagged HAs are potential candidates for gene-to-vaccine approaches to influenza vaccination. ..
- Chow C, Neto H, Foucart C, Moore I. Rab-A2 and Rab-A3 GTPases define a trans-golgi endosomal membrane domain in Arabidopsis that contributes substantially to the cell plate. Plant Cell. 2008;20:101-23 pubmed publisher..Despite the unique features of plant cytokinesis, membrane traffic to the division plane exhibits surprising molecular similarity across eukaryotic kingdoms in its reliance on Ypt3/Rab11/Rab-A GTPases. ..
- Blackman M, Fujioka H, Stafford W, Sajid M, Clough B, Fleck S, et al. A subtilisin-like protein in secretory organelles of Plasmodium falciparum merozoites. J Biol Chem. 1998;273:23398-409 pubmed..The subcellular location and timing of secretion of p47 suggest that it is likely to play a role in erythrocyte invasion. PfSUB-1 is a new potential target for antimalarial drug development. ..
- Wigge P, Kilmartin J. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001;152:349-60 pubmed..Thus the Ndc80p complex contains centromere-associated components conserved between yeasts and vertebrates. ..
- Yang S, Vickers E, Brehm A, Kouzarides T, Sharrocks A. Temporal recruitment of the mSin3A-histone deacetylase corepressor complex to the ETS domain transcription factor Elk-1. Mol Cell Biol. 2001;21:2802-14 pubmed..Elk-1 therefore undergoes temporal activator-repressor switching and contributes to both the activation and repression of target genes following growth factor stimulation. ..
- Mudher A, Chapman S, Richardson J, Asuni A, Gibb G, Pollard C, et al. Dishevelled regulates the metabolism of amyloid precursor protein via protein kinase C/mitogen-activated protein kinase and c-Jun terminal kinase. J Neurosci. 2001;21:4987-95 pubmed..Furthermore, we show that human dvl-1 and wnt-1 also reduce the phosphorylation of tau by GSK-3beta. Therefore, both APP metabolism and tau phosphorylation are potentially linked through wnt signaling. ..
- Allen S, Barrett C, Ray N, Robinson C. Essential cytoplasmic domains in the Escherichia coli TatC protein. J Biol Chem. 2002;277:10362-6 pubmed..The data point to two essential cytoplasmic domains on the TatC protein that are essential for export. ..
- Morrish B, Rumsby M. The 5' untranslated region of protein kinase Cdelta directs translation by an internal ribosome entry segment that is most active in densely growing cells and during apoptosis. Mol Cell Biol. 2002;22:6089-99 pubmed..Our studies suggest a mechanism whereby PKCdelta synthesis can be maintained under these conditions when cap-dependent translation is inhibited. ..
- Basu S, Totty N, Irwin M, Sudol M, Downward J. Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis. Mol Cell. 2003;11:11-23 pubmed..Akt phosphorylation of YAP may thus suppress the induction of the proapoptotic gene expression response following cellular damage. ..
- Hong Y, Stanley J, van Wezel R. Novel system for the simultaneous analysis of geminivirus DNA replication and plant interactions in Nicotiana benthamiana. J Virol. 2003;77:13315-22 pubmed..This defect correlates with possible loss of Rep nuclear localization and inability to trigger the host defense mechanism resembling a hypersensitive response. ..
- Holloway D, Chavali G, Hares M, Baker M, Subbarao G, Shapiro R, et al. Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41. Biochemistry. 2004;43:1230-41 pubmed..The loss of angiogenic activity is not attributable to disruption of known cell-binding or nuclear translocation sites but may be a consequence of the chimera's enhanced ribonucleolytic activity. ..
- Zecchini V, Domaschenz R, Winton D, Jones P. Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells. Genes Dev. 2005;19:1686-91 pubmed..This observation broadens the role of Notch into controlling postmitotic differentiation and indicates that the composition of the epithelium is not solely determined by progenitor cells. ..
- Sabbioneda S, Gourdin A, Green C, Zotter A, Giglia Mari G, Houtsmuller A, et al. Effect of proliferating cell nuclear antigen ubiquitination and chromatin structure on the dynamic properties of the Y-family DNA polymerases. Mol Biol Cell. 2008;19:5193-202 pubmed publisher..When DNA is exposed at replication forks, the polymerase residence times increase, and this is further facilitated by the ubiquitination of PCNA. ..
- Ludlow M, Durai L, Ennion S. Functional characterization of intracellular Dictyostelium discoideum P2X receptors. J Biol Chem. 2009;284:35227-39 pubmed publisher..Responses to the calmodulin antagonist calmidazolium, however, were reduced in p2xA/B/C/D/E null cells suggesting that dP2X receptors play a role in intracellular calcium signaling. ..
- Vladimirou E, Li M, Aldridge C, Frigerio L, Kirkilionis M, Robinson C. Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network. FEBS Lett. 2009;583:3690-6 pubmed publisher..Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming 'microdomains' that are sharply separated...
- Abdulrahman G. Therapeutic advances in the management of Huntington's disease. Yale J Biol Med. 2011;84:311-9 pubmed..Although there is intensive research into Huntington's disease and recent findings seem promising, effective therapeutic strategies may not be developed until the next few decades. ..
- Boudonck K, Dolan L, Shaw P. The movement of coiled bodies visualized in living plant cells by the green fluorescent protein. Mol Biol Cell. 1999;10:2297-307 pubmed..Deletion analysis of the U2B" gene construct has shown that the first RNP-80 motif is sufficient for localization to the coiled body. ..
- Draviam V, Orrechia S, Lowe M, Pardi R, Pines J. The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus. J Cell Biol. 2001;152:945-58 pubmed..Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity. ..
- Mitchell K, Pinton P, Varadi A, Tacchetti C, Ainscow E, Pozzan T, et al. Dense core secretory vesicles revealed as a dynamic Ca(2+) store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera. J Cell Biol. 2001;155:41-51 pubmed..The presence of RyRs on secretory vesicles suggests that local Ca(2+)-induced Ca(2+) release from vesicles docked at the plasma membrane could participate in triggering exocytosis. ..
- O Toole R, Smeulders M, Blokpoel M, Kay E, Lougheed K, Williams H. A two-component regulator of universal stress protein expression and adaptation to oxygen starvation in Mycobacterium smegmatis. J Bacteriol. 2003;185:1543-54 pubmed..Notably, the devR mutant was 10(4)-fold more sensitive than wild type to heat stress. We conclude that DevR is a stationary-phase regulator required for adaptation to oxygen starvation and resistance to heat stress in M. smegmatis. ..
- Benians A, Nobles M, Hosny S, Tinker A. Regulators of G-protein signaling form a quaternary complex with the agonist, receptor, and G-protein. A novel explanation for the acceleration of signaling activation kinetics. J Biol Chem. 2005;280:13383-94 pubmed
- Greaves J, Chamberlain L. Dual role of the cysteine-string domain in membrane binding and palmitoylation-dependent sorting of the molecular chaperone cysteine-string protein. Mol Biol Cell. 2006;17:4748-59 pubmed..These results reveal a dual function of the cysteine-string domain: initial membrane binding and palmitoylation-dependent sorting. ..
- French C, Boonstra B, Bufton K, Bruce N. Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens. J Bacteriol. 1997;179:2761-5 pubmed..STH is related to the flavoprotein disulfide oxidoreductases but lacks one of the conserved redox-active cysteine residues. The gene is highly similar to an E. coli gene of unknown function. ..
- Flynn P, Reece R. Activation of transcription by metabolic intermediates of the pyrimidine biosynthetic pathway. Mol Cell Biol. 1999;19:882-8 pubmed..These results are discussed in terms of (i) regulation of the pyrimidine biosynthetic pathway and (ii) a novel mechanism of regulating gene expression. ..
- Gillham H, Golding M, Pepperkok R, Gullick W. Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling. J Cell Biol. 1999;146:869-80 pubmed..Using green fluorescent protein-tagged fragments of p85 we show that binding to the receptor requires the NH(2)-terminal part of the protein as well as its SH2 domains. ..
- Pullinger G, Sowdhamini R, Lax A. Localization of functional domains of the mitogenic toxin of Pasteurella multocida. Infect Immun. 2001;69:7839-50 pubmed..The C-terminal end of PMT was predicted to be a mixed alpha/beta domain, a structure commonly found in catalytic domains. Homology to proteins of known structure and threading calculations supported these assignments. ..
- Sharma S, Pedley R, Bhatia J, Boxer G, El Emir E, Qureshi U, et al. Sustained tumor regression of human colorectal cancer xenografts using a multifunctional mannosylated fusion protein in antibody-directed enzyme prodrug therapy. Clin Cancer Res. 2005;11:814-25 pubmed..These results provide a basis for the next stage in clinical development of ADEPT. ..
- Rodriguez Mendieta I, Spence G, Gell C, Radford S, Smith D. Ultraviolet resonance Raman studies reveal the environment of tryptophan and tyrosine residues in the native and partially folded states of the E colicin-binding immunity protein Im7. Biochemistry. 2005;44:3306-15 pubmed..We corroborated this observation using tryptophan-containing model compounds and conclude that the conventional interpretation of this UVRR feature at these wavelengths is unreliable. ..
- Graham M, Edwards M, Holden Dye L, Morgan A, Burgoyne R, Barclay J. UNC-18 modulates ethanol sensitivity in Caenorhabditis elegans. Mol Biol Cell. 2009;20:43-55 pubmed publisher..We conclude that ethanol acts, at least partially, at the level of vesicle fusion and that its acute effects are ameliorated by point mutations in UNC-18. ..
- Mashanov G, Nobles M, Harmer S, Molloy J, Tinker A. Direct observation of individual KCNQ1 potassium channels reveals their distinctive diffusive behavior. J Biol Chem. 2010;285:3664-75 pubmed publisher..This mutant has been identified in patients with the long QT syndrome. We propose that KCNQ1-KCNE1 complexes interact intermittently with the actin cytoskeleton via the C-terminal region and this interaction may have a functional role...
- Greaves J, Carmichael J, Chamberlain L. The palmitoyl transferase DHHC2 targets a dynamic membrane cycling pathway: regulation by a C-terminal domain. Mol Biol Cell. 2011;22:1887-95 pubmed publisher..This study is the first to highlight dynamic cycling of a mammalian DHHC protein between clearly defined membrane compartments, and to identify domains that specify membrane targeting of this protein family. ..
- Gabernet Castello C, DuBois K, Nimmo C, Field M. Rab11 function in Trypanosoma brucei: identification of conserved and novel interaction partners. Eukaryot Cell. 2011;10:1082-94 pubmed publisher..These data suggest that Rab11 functions are mediated by evolutionarily conserved (i.e., AZI1 and Sec15) and potentially lineage-specific (RBP74) interactions essential for the integration of the endomembrane system. ..
- Sözen M, Armstrong J, Yang M, Kaiser K, Dow J. Functional domains are specified to single-cell resolution in a Drosophila epithelium. Proc Natl Acad Sci U S A. 1997;94:5207-12 pubmed..Building upon recent advances in Drosophila Malpighian tubule physiology, the present study confirms this tissue as a singular model for integrative physiology...
- Chen D, Lucey M, Phoenix F, Lopez Garcia J, Hart S, Losson R, et al. T:G mismatch-specific thymine-DNA glycosylase potentiates transcription of estrogen-regulated genes through direct interaction with estrogen receptor alpha. J Biol Chem. 2003;278:38586-92 pubmed..Together with recent reports linking TFIIH in regulating NR function, our findings provide new data to further support an important link between DNA repair proteins and nuclear receptor function. ..
- Barcaroli D, Dinsdale D, Neale M, Bongiorno Borbone L, Ranalli M, Munarriz E, et al. FLASH is an essential component of Cajal bodies. Proc Natl Acad Sci U S A. 2006;103:14802-7 pubmed..Because the function of FLASH in the apoptosis receptor signaling pathway has been strongly questioned, we have now identified a clear function for this protein. ..
- Silva J, Lelianova V, Hopkins C, Volynski K, Ushkaryov Y. Functional cross-interaction of the fragments produced by the cleavage of distinct adhesion G-protein-coupled receptors. J Biol Chem. 2009;284:6495-506 pubmed publisher..This unusual cross-assembly of aGPCR fragments could couple cell-surface interactions to multiple signaling pathways. ..
- Little S, Hyde S, Campbell C, Lilley R, Robinson M. Translational coupling in the threonine operon of Escherichia coli K-12. J Bacteriol. 1989;171:3518-22 pubmed..In addition, it was found that a sequence between 220 and 57 base pairs before the start of thrB was necessary to allow translational coupling to occur. ..
- Verderio E, Nicholas B, Gross S, Griffin M. Regulated expression of tissue transglutaminase in Swiss 3T3 fibroblasts: effects on the processing of fibronectin, cell attachment, and cell death. Exp Cell Res. 1998;239:119-38 pubmed
- Rodriguez M, Dargemont C, Hay R. SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting. J Biol Chem. 2001;276:12654-9 pubmed..Thus, protein substrates must be targeted to the nucleus to undergo SUMO-1 conjugation. ..
- Matthews D. Adenovirus protein V induces redistribution of nucleolin and B23 from nucleolus to cytoplasm. J Virol. 2001;75:1031-8 pubmed..Moreover, adenovirus protein V is the first protein demonstrated to be capable of redirecting nucleolin and B23 to the cytoplasm. ..
- Mattock H, Lane D, Warbrick E. Inhibition of cell proliferation by the PCNA-binding region of p21 expressed as a GFP miniprotein. Exp Cell Res. 2001;265:234-41 pubmed..The capacity of this peptide sequence to suppress cell proliferation in vivo is of interest as the basis for the design of potential antiproliferative therapeutic agents. ..
- AbdelRaheim S, Cartwright J, Gasmi L, McLennan A. The NADH diphosphatase encoded by the Saccharomyces cerevisiae NPY1 nudix hydrolase gene is located in peroxisomes. Arch Biochem Biophys. 2001;388:18-24 pubmed..This function may be the regulation of nicotinamide coenzyme concentrations independently of those in other compartments or the elimination of oxidized nucleotide derivatives from the peroxisomal environment. ..
- Ross K, Yang L, Dower S, Volpe F, Guesdon F. Identification of threonine 66 as a functionally critical residue of the interleukin-1 receptor-associated kinase. J Biol Chem. 2002;277:37414-21 pubmed..These properties in turn determine key aspects of the signaling function of IRAK. ..
- O Luanaigh N, Pardo R, Fensome A, Allen Baume V, Jones D, Holt M, et al. Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells. Mol Biol Cell. 2002;13:3730-46 pubmed..We conclude that both activation of ARF6 by antigen and a continual PLD2 activity are essential for local phosphatidylinositol(4,5)bisphosphate generation that regulates dynamic actin cytoskeletal rearrangements. ..
- Strutt H, Strutt D. Nonautonomous planar polarity patterning in Drosophila: dishevelled-independent functions of frizzled. Dev Cell. 2002;3:851-63 pubmed..We also find evidence that polarity information may be propagated by modulation of the binding affinities of the cadherins encoded by the ds and ft loci. ..
- Yu D, Sonoda E, Takeda S, Huang C, Pellegrini L, Blundell T, et al. Dynamic control of Rad51 recombinase by self-association and interaction with BRCA2. Mol Cell. 2003;12:1029-41 pubmed
- Hamada F, Bienz M. The APC tumor suppressor binds to C-terminal binding protein to divert nuclear beta-catenin from TCF. Dev Cell. 2004;7:677-85 pubmed..Our evidence indicates that APC is an adaptor between beta-catenin and CtBP and that CtBP lowers the availability of free nuclear beta-catenin for binding to TCF by sequestering APC/beta-catenin complexes. ..
- Donnelly M, Verhagen J, Elliott G. RNA binding by the herpes simplex virus type 1 nucleocytoplasmic shuttling protein UL47 is mediated by an N-terminal arginine-rich domain that also functions as its nuclear localization signal. J Virol. 2007;81:2283-96 pubmed publisher..Using RNA immunoprecipitations, we demonstrate that hUL47 is also bound in vivo to at least one viral transcript, the ICP0 mRNA. Taken together, these results suggest that hUL47 may play a role in RNA biogenesis in the infected cell...
- Serrels A, Timpson P, Canel M, Schwarz J, Carragher N, Frame M, et al. Real-time study of E-cadherin and membrane dynamics in living animals: implications for disease modeling and drug development. Cancer Res. 2009;69:2714-9 pubmed publisher..Furthermore, this work highlights critical differences in molecular dynamics in vitro and in vivo, which have important implications for the use of cultured disease models as surrogates for living tissue. ..
- Verstak B, Arnot C, Gay N. An alanine-to-proline mutation in the BB-loop of TLR3 Toll/IL-1R domain switches signalling adaptor specificity from TRIF to MyD88. J Immunol. 2013;191:6101-9 pubmed publisher..These results show that adaptor specificity can be conferred by minimal determinants of the Toll/IL-1R domain. ..
- Spring F, Parsons S, Ortlepp S, Olsson M, Sessions R, Brady R, et al. Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms. Blood. 2001;98:458-66 pubmed..These findings suggest a role for ICAM-4 in normal erythropoiesis and may also be relevant to the adhesive interactions of sickle cells. ..
- Keller C, Krude T. Requirement of Cyclin/Cdk2 and protein phosphatase 1 activity for chromatin assembly factor 1-dependent chromatin assembly during DNA synthesis. J Biol Chem. 2000;275:35512-21 pubmed..These data provide functional evidence for a requirement of the cell cycle machinery for nucleosome assembly by CAF-1 during DNA replication. ..
- Parsons M, Keppler M, Kline A, Messent A, Humphries M, Gilchrist R, et al. Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxis. Mol Cell Biol. 2002;22:5897-911 pubmed..Importantly, our findings outline a new concept as to how carcinoma cell chemotaxis is enhanced and provide a conceptual basis for interfering with tumor cell dissemination. ..
- Oeffinger M, Leung A, Lamond A, Tollervey D, Lueng A. Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis. RNA. 2002;8:626-36 pubmed..Nop7p is a component of at least three different pre-60S particles, and we propose that it carries out distinct functions in each of these complexes. ..
- Wilcox A, Katsanakis K, Bheda F, Pillay T. Asb6, an adipocyte-specific ankyrin and SOCS box protein, interacts with APS to enable recruitment of elongins B and C to the insulin receptor signaling complex. J Biol Chem. 2004;279:38881-8 pubmed..We conclude that Asb6 functions to regulate components of the insulin signaling pathway in adipocytes by facilitating degradation by the APS-dependent recruitment of Asb6 and Elongins BC. ..
- Zhang J, Raper A, Sugita N, Hingorani R, Salio M, Palmowski M, et al. Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors. Blood. 2006;107:3600-8 pubmed..Targeting Siglec-H may therefore be a useful way of delivering antigens to pDC precursors for cross-presentation. ..
- Liang J, Fantes P. The Schizosaccharomyces pombe Cdc7 protein kinase required for septum formation is a client protein of Cdc37. Eukaryot Cell. 2007;6:1089-96 pubmed..Other evidence suggests that, in addition to mitosis and septum initiation, Cdc37 may also be required for septum cleavage. ..
- Dukes J, Fish L, Richardson J, Blaikley E, Burns S, Caunt C, et al. Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells. Mol Biol Cell. 2011;22:3192-205 pubmed publisher..Thus, in mammalian epithelial cells, ESCRT function is required for claudin-1 trafficking and for epithelial cell polarity, supporting the hypothesis that ESCRT proteins function as tumor suppressors. ..
- Glynne P, Darling K, Picot J, Evans T. Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output. J Biol Chem. 2002;277:33132-8 pubmed..These studies define human epithelial iNOS as an apical EBP50-binding protein and suggest that the physical association of iNOS with EBP50 might allow precise NO modulation of EBP50-associated protein functions. ..
- Vega Lopez F, Brooks L, Dockrell H, De Smet K, Thompson J, Hussain R, et al. Sequence and immunological characterization of a serine-rich antigen from Mycobacterium leprae. Infect Immun. 1993;61:2145-53 pubmed..leprae antigen. In contrast, all serum samples from endemic controls were negative, while 26% of serum samples from tuberculosis patients were weakly positive. ..
- Conway S, Drew J, Mowat E, Barrett P, Delagrange P, Morgan P. Chimeric melatonin mt1 and melatonin-related receptors. Identification of domains and residues participating in ligand binding and receptor activation of the melatonin mt1 receptor. J Biol Chem. 2000;275:20602-9 pubmed..This identified Gly(258) (Gly(6.55)) as a critical residue required for high affinity ligand binding and receptor function. ..
- Hardie R, Gu Y, Martin F, Sweeney S, Raghu P. In vivo light-induced and basal phospholipase C activity in Drosophila photoreceptors measured with genetically targeted phosphatidylinositol 4,5-bisphosphate-sensitive ion channels (Kir2.1). J Biol Chem. 2004;279:47773-82 pubmed
- Scholpp S, Delogu A, Gilthorpe J, Peukert D, Schindler S, Lumsden A. Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proc Natl Acad Sci U S A. 2009;106:19895-900 pubmed publisher..Thus, the presence or absence of a single upstream regulator of proneural gene expression, Her6, leads to the establishment of discrete neuronal domains in the thalamus. ..
- Tate C, Henderson P. Membrane topology of the L-rhamnose-H+ transport protein (RhaT) from enterobacteria. J Biol Chem. 1993;268:26850-7 pubmed..However, the localization of the putative first hydrophilic loop and the NH2 terminus was not possible because the beta-lactamase fusions in this region were shown to be unreliable indicators of the topology of RhaT. ..
- Galanis A, Yang S, Sharrocks A. Selective targeting of MAPKs to the ETS domain transcription factor SAP-1. J Biol Chem. 2001;276:965-73 pubmed..Our data therefore demonstrate that a modular system of motifs is responsible for directing specific MAPK subtypes to SAP-1, but also point to important distinctions in the mechanism of action of the D-domain and FXF motif. ..
- Adams J, Kureishy N, Taylor A. A role for syndecan-1 in coupling fascin spike formation by thrombospondin-1. J Cell Biol. 2001;152:1169-82 pubmed..These results establish a novel role for syndecan-1 protein in coupling a physiological matrix ligand to formation of a specific matrix contact structure. ..
- Clarkson R, Boland M, Kritikou E, Lee J, Freeman T, Tiffen P, et al. The genes induced by signal transducer and activators of transcription (STAT)3 and STAT5 in mammary epithelial cells define the roles of these STATs in mammary development. Mol Endocrinol. 2006;20:675-85 pubmed
- Kepinski S, Leyser O. Auxin-induced SCFTIR1-Aux/IAA interaction involves stable modification of the SCFTIR1 complex. Proc Natl Acad Sci U S A. 2004;101:12381-6 pubmed..Instead, we present data suggesting that auxin promotes the SCF(TIR1)-Aux/IAA interaction by affecting the SCF component, TIR1, or proteins tightly associated with it. ..
- Bassi R, Burgoyne J, DeNicola G, Rudyk O, DeSantis V, Charles R, et al. Redox-dependent dimerization of p38Î± mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3. J Biol Chem. 2017;292:16161-16173 pubmed publisher..Our findings suggest that cysteine residues within p38Î± act as redox sensors that can dynamically regulate the association between p38 and MKK3. ..
- Golby P, Kelly D, Guest J, Andrews S. Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli. J Bacteriol. 1998;180:6586-96 pubmed..The expression of dcuB is entirely consistent with a primary role for DcuB in mediating C4-dicarboxylate transport during anaerobic fumarate respiration. The precise physiological purpose of DcuA remains unclear. ..
- Christian M, Tullet J, Parker M. Characterization of four autonomous repression domains in the corepressor receptor interacting protein 140. J Biol Chem. 2004;279:15645-51 pubmed..Use of specific inhibitors indicates that RD2, RD3, and RD4 are capable of functioning by HDAC-dependent and HDAC-independent mechanisms, depending upon cell type. ..
- Barreca C, O Hare P. Suppression of herpes simplex virus 1 in MDBK cells via the interferon pathway. J Virol. 2004;78:8641-53 pubmed..We believe the conclusions have significant implications for the study of HSV-1 and interferon signaling both in culture and in animal models. ..
- Kwok C, Zeisig B, Dong S, So C. Forced homo-oligomerization of RARalpha leads to transformation of primary hematopoietic cells. Cancer Cell. 2006;9:95-108 pubmed..Taken together, these findings endorse an oligomerization-dependent mechanism for RARalpha-mediated transformation and suggest a potential avenue for molecular therapy. ..
- Eastmond P. SUGAR-DEPENDENT1 encodes a patatin domain triacylglycerol lipase that initiates storage oil breakdown in germinating Arabidopsis seeds. Plant Cell. 2006;18:665-75 pubmed..These data shed light on the mechanism of lipolysis in plants and establish that a central component is evolutionarily conserved among eukaryotes. ..
- Schornack S, Van Damme M, Bozkurt T, Cano L, Smoker M, Thines M, et al. Ancient class of translocated oomycete effectors targets the host nucleus. Proc Natl Acad Sci U S A. 2010;107:17421-6 pubmed publisher..Finally, this work further implicates the host nucleus as an important cellular compartment where the fate of plant-microbe interactions is determined...
- Utton M, Connell J, Asuni A, van Slegtenhorst M, Hutton M, de Silva R, et al. The slow axonal transport of the microtubule-associated protein tau and the transport rates of different isoforms and mutants in cultured neurons. J Neurosci. 2002;22:6394-400 pubmed
- Sewell R, Bäckström M, Dalziel M, Gschmeissner S, Karlsson H, Noll T, et al. The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer. J Biol Chem. 2006;281:3586-94 pubmed..3 to 3.8 per tandem repeat. The availability of large quantities of this MUC1 glycoform will allow the evaluation of its efficacy as an immunogen for immunotherapy of MUC1/STn-expressing tumors. ..
- Lee S, Faux C, Nixon J, Alete D, Chilton J, Hawadle M, et al. Dimerization of protein tyrosine phosphatase sigma governs both ligand binding and isoform specificity. Mol Cell Biol. 2007;27:1795-808 pubmed..These findings have important implications for our understanding of how heterophilic ligands interact with, and potentially regulate, RPTPs. ..
- Koprivova A, des Francs Small C, Calder G, Mugford S, Tanz S, Lee B, et al. Identification of a pentatricopeptide repeat protein implicated in splicing of intron 1 of mitochondrial nad7 transcripts. J Biol Chem. 2010;285:32192-9 pubmed publisher..The same phenotype was observed with other mutants affected in function of Complex I, thus reinforcing the importance of Complex I function for cellular redox homeostasis. ..
- Pawliczek T, Crump C. Herpes simplex virus type 1 production requires a functional ESCRT-III complex but is independent of TSG101 and ALIX expression. J Virol. 2009;83:11254-64 pubmed publisher..In line with these data, we also find that ESCRT-III proteins and VPS4A/B are specifically incorporated into mature HSV-1 virions. ..
- van der Stegen S, Davies D, Wilkie S, Foster J, Sosabowski J, Burnet J, et al. Preclinical in vivo modeling of cytokine release syndrome induced by ErbB-retargeted human T cells: identifying a window of therapeutic opportunity?. J Immunol. 2013;191:4589-98 pubmed publisher..Furthermore, our findings argue that ErbB-retargeted T cells can achieve therapeutic benefit in the absence of unacceptable toxicity, providing that route of administration and dose are carefully optimized. ..
- Holbrook J, Danpure C. Molecular basis for the dual mitochondrial and cytosolic localization of alanine:glyoxylate aminotransferase in amphibian liver cells. J Biol Chem. 2002;277:2336-44 pubmed..These results are discussed not only in terms of the molecular evolution of AGT targeting but also in terms of the ancillary requirements for the peroxisomal targeting of human AGT. ..
- Way G, Morrice N, Smythe C, O Sullivan A. Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells. Mol Biol Cell. 2002;13:3344-54 pubmed..Secernin 1 increases both the extent of secretion and increases the sensitivity of mast cells to stimulation with calcium. ..
- Higgins L, McDonald S, Whittle N, Crockett N, Shields J, MacDonald T. Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein. J Immunol. 1999;162:486-93 pubmed..Targeting OX40 therefore shows considerable promise as a new strategy to inhibit ongoing T cell reactions in the gut. ..
- Ju M, Stevens L, Leadbitter E, Wray D. The Roles of N- and C-terminal determinants in the activation of the Kv2.1 potassium channel. J Biol Chem. 2003;278:12769-78 pubmed
- Yang S, Sharrocks A. SUMO promotes HDAC-mediated transcriptional repression. Mol Cell. 2004;13:611-7 pubmed..Thus, our data demonstrate an important integration point for two protein-modifying pathways in the cell, the SUMO and deacetylation pathways, that combine to promote transcriptional repression. ..