Experts and Doctors on protein binding in Germany

Summary

Locale: Germany
Topic: protein binding

Top Publications

  1. Bremser M, Nickel W, Schweikert M, Ravazzola M, Amherdt M, Hughes C, et al. Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. Cell. 1999;96:495-506 pubmed
  2. Umeda A, Meyerholz A, Ungewickell E. Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation. Eur J Cell Biol. 2000;79:336-42 pubmed
    ..The cellular distribution of membrane-associated adaptors was unaffected by overexpression of GAK. Our results point to a hsc70/auxilin-based uncoating system as a ubiquitous feature of eukaryotic cells...
  3. Kleemann R, Hausser A, Geiger G, Mischke R, Burger Kentischer A, Flieger O, et al. Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1. Nature. 2000;408:211-6 pubmed
    ..We conclude that MIF may act broadly to negatively regulate Jab1-controlled pathways and that the MIF-Jab1 interaction may provide a molecular basis for key activities of MIF. ..
  4. Richter K, Reinstein J, Buchner J. N-terminal residues regulate the catalytic efficiency of the Hsp90 ATPase cycle. J Biol Chem. 2002;277:44905-10 pubmed
    ..We propose that the turnover rate of Hsp90 is decisively controlled by intermolecular interactions between the N-terminal domains. ..
  5. Walders Harbeck B, Khaitlina S, Hinssen H, Jockusch B, Illenberger S. The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS Lett. 2002;529:275-80 pubmed
    ..Phosphorylation negatively affects VASP/G-actin interactions preventing VASP-induced actin filament formation. ..
  6. Stegmuller J, Werner H, Nave K, Trotter J. The proteoglycan NG2 is complexed with alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors by the PDZ glutamate receptor interaction protein (GRIP) in glial progenitor cells. Implications for glial-neuronal signaling. J Biol Chem. 2003;278:3590-8 pubmed
    ..AMPA receptor complex in glial-neuronal recognition and signaling. ..
  7. Wegele H, Haslbeck M, Reinstein J, Buchner J. Sti1 is a novel activator of the Ssa proteins. J Biol Chem. 2003;278:25970-6 pubmed
    ..Analysis of the underlying activation mechanism revealed that ATP hydrolysis is rate-limiting in the Ssa1 ATPase cycle and that this step is accelerated by Sti1. Thus, Sti1 is a potent novel effector for the Hsp70 ATPase. ..
  8. Köster M, Lykke Andersen S, Elnakady Y, Gerth K, Washausen P, Hofle G, et al. Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. Exp Cell Res. 2003;286:321-31 pubmed
    ..Thus, the ratjadones represent a new class of natural compounds which inhibit proliferation in eukaryotes by blocking nuclear export. ..
  9. Andag U, Schmitt H. Dsl1p, an essential component of the Golgi-endoplasmic reticulum retrieval system in yeast, uses the same sequence motif to interact with different subunits of the COPI vesicle coat. J Biol Chem. 2003;278:51722-34 pubmed
    ..They interact with different layers of the vesicle coat by using tandemly arranged sequence motifs, some of which have dual specificity. ..

More Information

Publications803 found, 100 shown here

  1. Borrmann L, Schwanbeck R, Heyduk T, Seebeck B, Rogalla P, Bullerdiek J, et al. High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity. Nucleic Acids Res. 2003;31:6841-51 pubmed
    ..Our results provide the first insights into how HMGA2 and its aberrant forms bind and regulate the ERCC1 promoter. ..
  2. Schreuder H, Liesum A, Pohl J, Kruse M, Koyama M. Crystal structure of recombinant human growth and differentiation factor 5: evidence for interaction of the type I and type II receptor-binding sites. Biochem Biophys Res Commun. 2005;329:1076-86 pubmed
  3. Hägele S, Behnam B, Borter E, Wolfe J, Paasch U, Lukashev D, et al. TSGA10 prevents nuclear localization of the hypoxia-inducible factor (HIF)-1alpha. FEBS Lett. 2006;580:3731-8 pubmed
    ..HIF-1alpha nuclear localization and HIF-1 transcriptional activity were significantly affected by overexpressed TSGA10. ..
  4. Ritschel T, Hoertner S, Heine A, Diederich F, Klebe G. Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT. Chembiochem. 2009;10:716-27 pubmed publisher
    ..Docking suggests multiple orientations of these side chains. Obviously, an entropic advantage of the residual mobility experienced by these ligands in the bound state is beneficial and reveals an overall improved protein binding...
  5. Surmann Schmitt C, Widmann N, Dietz U, Saeger B, Eitzinger N, Nakamura Y, et al. Wif-1 is expressed at cartilage-mesenchyme interfaces and impedes Wnt3a-mediated inhibition of chondrogenesis. J Cell Sci. 2009;122:3627-37 pubmed publisher
    ..These results identify Wif-1 as a novel extracellular Wnt modulator in cartilage biology. ..
  6. Naumann A, Hochstein N, Weber S, Fanning E, Doerfler W. A distinct DNA-methylation boundary in the 5'- upstream sequence of the FMR1 promoter binds nuclear proteins and is lost in fragile X syndrome. Am J Hum Genet. 2009;85:606-16 pubmed publisher
    ..They may arise during de novo methylation only in DNA that is organized in chromatin and be due to the binding of specific proteins. ..
  7. Splinter J, Jakob B, Lang M, Yano K, Engelhardt J, Hell S, et al. Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci. Mutagenesis. 2010;25:289-97 pubmed publisher
    ..Hence, our results indicate that the local dose delivered by UVA-LMI is extremely high and cannot be accurately translated into an equivalent ionizing radiation dose, despite the sophisticated techniques used in this study. ..
  8. Schroder S, Surmann P. Photoallergenic potential testing by online irradiation and HPLC. Pharmazie. 2010;65:339-42 pubmed
    ..The screening was performed at pH 3 and pH 7. Both cleavage of the peptide binding and formation of new products could indicate a photoallergenic potential...
  9. Lorenz S, Schmid F. Reprogramming the infection mechanism of a filamentous phage. Mol Microbiol. 2011;80:827-34 pubmed publisher
    ..The redesigned phage fd was robust and as infectious as wild-type phage fd...
  10. Samsonov S, Teyra J, Pisabarro M. Docking glycosaminoglycans to proteins: analysis of solvent inclusion. J Comput Aided Mol Des. 2011;25:477-89 pubmed publisher
    ..Our work highlights the current challenges of introducing solvent in protein-GAGs recognition studies, which is crucial for exploiting the full potential of these molecules for rational engineering. ..
  11. Walter B, Nordhoff C, Varga G, Goncharenko G, Schneider S, Ludwig S, et al. Mss4 protein is a regulator of stress response and apoptosis. Cell Death Dis. 2012;3:e297 pubmed publisher
  12. Ohrt T, Prior M, Dannenberg J, Odenwälder P, Dybkov O, Rasche N, et al. Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. RNA. 2012;18:1244-56 pubmed publisher
    ..Moreover, dcFCCS represents a powerful tool ideally suited to study quantitatively spliceosomal protein dynamics in equilibrium...
  13. Dove S, Danker K, Stasch J, Kaever V, Seifert R. Structure/activity relationships of (M)ANT- and TNP-nucleotides for inhibition of rat soluble guanylyl cyclase ?1?1. Mol Pharmacol. 2014;85:598-607 pubmed publisher
    ..Moreover, it may become possible to develop mAC inhibitors with selectivity relative to sGC. ..
  14. Grotwinkel J, Wild K, Segnitz B, Sinning I. SRP RNA remodeling by SRP68 explains its role in protein translocation. Science. 2014;344:101-4 pubmed publisher
    ..The ARM opens the conserved 5f RNA loop, which in ribosome-bound SRP establishes a contact to ribosomal RNA. Our data provide the structural basis for eukaryote-specific, SRP68-driven RNA remodeling required for protein translocation. ..
  15. Springer C, Kunzler M, Balmelli T, Braus G. Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter. J Biol Chem. 1996;271:29637-43 pubmed
    ..These results suggest that GCN4p and BAS1/2p are able to act simultaneously through the same DNA sequence in vivo and use this site independently from each other in a non-exclusive manner. ..
  16. Wrenger S, Hoffmann T, Faust J, Mrestani Klaus C, Brandt W, Neubert K, et al. The N-terminal structure of HIV-1 Tat is required for suppression of CD26-dependent T cell growth. J Biol Chem. 1997;272:30283-8 pubmed
    ..Our data suggest that the N-terminal residues of HIV-1 Tat do interact directly with the active site of DP IV and that DP IV does mediate Tat's immunosuppressive effects. ..
  17. Oertel R, Rahn R, Kirch W. Clinical pharmacokinetics of articaine. Clin Pharmacokinet. 1997;33:417-25 pubmed
    ..The plasma protein binding rate of articaine and articainic acid is 70%. It has been concluded that an unintentional intravascular injection of articaine 80 mg does not cause toxic effects in healthy individuals. ..
  18. Rother M, Wilting R, Commans S, B ck A. Identification and characterisation of the selenocysteine-specific translation factor SelB from the archaeon Methanococcus jannaschii. J Mol Biol. 2000;299:351-8 pubmed publisher
  19. Wirtz M, Berkowitz O, Droux M, Hell R. The cysteine synthase complex from plants. Mitochondrial serine acetyltransferase from Arabidopsis thaliana carries a bifunctional domain for catalysis and protein-protein interaction. Eur J Biochem. 2001;268:686-93 pubmed
    ..The bifunctionality of the SAT C-terminus in transferase activity and interaction with OAS-TL is demonstrated and discussed with respect to the putative role of the cysteine synthase complex in regulation of cysteine biosynthesis. ..
  20. Ammendola A, Geiselhöringer A, Hofmann F, Schlossmann J. Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta. J Biol Chem. 2001;276:24153-9 pubmed
    ..These results show that cGMP induced reduction of cytosolic calcium concentrations requires cGKIbeta and phosphorylation of Ser(696) of IRAG. ..
  21. Vilardaga J, Frank M, Krasel C, Dees C, Nissenson R, Lohse M. Differential conformational requirements for activation of G proteins and the regulatory proteins arrestin and G protein-coupled receptor kinase in the G protein-coupled receptor for parathyroid hormone (PTH)/PTH-related protein. J Biol Chem. 2001;276:33435-43 pubmed
    ..Furthermore, PTHR sequestration does not appear to require G protein activation. ..
  22. Hopf M, Göhring W, Ries A, Timpl R, Hohenester E. Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1. Nat Struct Biol. 2001;8:634-40 pubmed
    ..A large surface patch on the beta-barrel is strikingly conserved in all metazoan nidogens. Site-directed mutagenesis demonstrates that the conserved residues are involved in perlecan binding. ..
  23. Bailer S, Balduf C, Hurt E. The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol Cell Biol. 2001;21:7944-55 pubmed
    ..Thus, distinct coiled-coil regions within Nsp1p-C have separate functions that are related to the assembly of different NPC subcomplexes, nucleocytoplasmic transport, and incorporation into the nuclear pores. ..
  24. Pohl E, Brunner N, Wilmanns M, Hensel R. The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax. J Biol Chem. 2002;277:19938-45 pubmed
  25. Lorenz K, Lohse M, Quitterer U. Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2. Nature. 2003;426:574-9 pubmed
    ..A physiological role for this mechanism is shown in cardiomyocytes in which the downregulation of RKIP restrains beta-adrenergic signalling and contractile activity. ..
  26. Habich C, Kempe K, Burkart V, van der Zee R, Lillicrap M, Gaston H, et al. Identification of the heat shock protein 60 epitope involved in receptor binding on macrophages. FEBS Lett. 2004;568:65-9 pubmed
    ..Monoclonal antibodies addressing regions aa1-200, aa335-366 or aa383-447 did not block HSP60 binding. We conclude that a single C-terminal region, aa481-500, accounts for the binding of HSP60 to macrophages. ..
  27. Aufhammer S, Warkentin E, Ermler U, Hagemeier C, Thauer R, Shima S. Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420: Architecture of the F420/FMN binding site of enzymes within the nonprolyl cis-peptide containing bacterial luciferase family. Protein Sci. 2005;14:1840-9 pubmed publisher
  28. Tholema N, Vor der Brüggen M, Maser P, Nakamura T, Schroeder J, Kobayashi H, et al. All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus. J Biol Chem. 2005;280:41146-54 pubmed
    ..They also indicate that the role of KtrA lies in conferring velocity and ion coupling to the Ktr complex...
  29. Fromme T, Reichwald K, Platzer M, Li X, Klingenspor M. Chicken ovalbumin upstream promoter transcription factor II regulates uncoupling protein 3 gene transcription in Phodopus sungorus. BMC Mol Biol. 2007;8:1 pubmed
    ..We therefore investigated whether Coup-TFII is a further player in the transcriptional control of the Ucp3 gene in rodents...
  30. Siepe D, Jentsch S. Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation. Nat Cell Biol. 2009;11:967-72 pubmed publisher
    ..This suggests that the Pin1 activity is often translated into a fate-determining ubiquitylation switch, and that Pin1 may affect the degree of substrate ubiquitylation in other pathways as well. ..
  31. Coutandin D, Ou H, Lohr F, Dötsch V. Tracing the protectors path from the germ line to the genome. Proc Natl Acad Sci U S A. 2010;107:15318-25 pubmed publisher
    ..This suggests that p53 is the most recent evolutionary member of this protein family and predicts a mechanism for p53 activation. ..
  32. Fuchs N, Kraft M, Tondera C, Hanschmann K, Lower J, Löwer R. Expression of the human endogenous retrovirus (HERV) group HML-2/HERV-K does not depend on canonical promoter elements but is regulated by transcription factors Sp1 and Sp3. J Virol. 2011;85:3436-48 pubmed publisher
  33. Prasad S, Bocola M, Reetz M. Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis. Chemphyschem. 2011;12:1550-7 pubmed publisher
    ..A theoretical investigation using molecular dynamics simulations and docking experiments revealed the source of enhanced activity and stereoselectivity. ..
  34. Hagn F, Lagleder S, Retzlaff M, Rohrberg J, Demmer O, Richter K, et al. Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. Nat Struct Mol Biol. 2011;18:1086-93 pubmed publisher
    ..The fine-tuned interplay between several Hsp90 domains provides the interactions required for efficient chaperoning of p53. ..
  35. Eicher T, Cha H, Seeger M, Brandstätter L, El Delik J, Bohnert J, et al. Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop. Proc Natl Acad Sci U S A. 2012;109:5687-92 pubmed publisher
  36. Klopffleisch K, Issinger O, Niefind K. Low-density crystal packing of human protein kinase CK2 catalytic subunit in complex with resorufin or other ligands: a tool to study the unique hinge-region plasticity of the enzyme without packing bias. Acta Crystallogr D Biol Crystallogr. 2012;68:883-92 pubmed publisher
    ..Ligands that stabilize either the open or the closed conformation by hydrogen bonds are known, but a general rule is not yet apparent. ..
  37. Battenberg O, Yang Y, Verhelst S, Sieber S. Target profiling of 4-hydroxyderricin in S. aureus reveals seryl-tRNA synthetase binding and inhibition by covalent modification. Mol Biosyst. 2013;9:343-51 pubmed publisher
    ..This modification results in inhibition of the amino acylation of tRNAs catalyzed by S. aureus STS which is an essential enzymatic pathway for bacterial viability. ..
  38. Kerkhoff C, Vogl T, Nacken W, Sopalla C, Sorg C. Zinc binding reverses the calcium-induced arachidonic acid-binding capacity of the S100A8/A9 protein complex. FEBS Lett. 1999;460:134-8 pubmed
    ..Due to the fact that the inhibitory effect of Zn2+ was present at physiological serum concentrations, it is assumed that released S100A8/A9 may carry AA at inflammatory lesions, but not within the blood compartment. ..
  39. Siniossoglou S, Lutzmann M, Santos Rosa H, Leonard K, Mueller S, Aebi U, et al. Structure and assembly of the Nup84p complex. J Cell Biol. 2000;149:41-54 pubmed
    ..Thus, the Nup84p complex constitutes a paradigm of an NPC structural module with distinct composition, structure, and a role in nuclear mRNA export and NPC bio- genesis. ..
  40. Ries A, Göhring W, Fox J, Timpl R, Sasaki T. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies. Eur J Biochem. 2001;268:5119-28 pubmed
    ..Three of the antibodies showed strongly reduced binding to G2 and G3 after complex formation with either a perlecan domain or laminin-1. ..
  41. Warkentin E, Mamat B, Sordel Klippert M, Wicke M, Thauer R, Iwata M, et al. Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound. EMBO J. 2001;20:6561-9 pubmed publisher
    ..1 A, which is optimal for hydride transfer. Comparison of the structures bound with and without substrates reveals that of the two substrates NADP has to bind first, the binding being associated with an induced fit...
  42. Ziegler W, Tigges U, Zieseniss A, Jockusch B. A lipid-regulated docking site on vinculin for protein kinase C. J Biol Chem. 2002;277:7396-404 pubmed
  43. Pabst S, Margittai M, Vainius D, Langen R, Jahn R, Fasshauer D. Rapid and selective binding to the synaptic SNARE complex suggests a modulatory role of complexins in neuroexocytosis. J Biol Chem. 2002;277:7838-48 pubmed
    ..Furthermore, complexin did not promote the formation of SNARE complex oligomers. Together, our data suggest that complexins modulate neuroexocytosis after assembly of membrane-bridging SNARE complexes. ..
  44. Hennige A, Fritsche A, Strack V, Weigert C, Mischak H, Borboni P, et al. PKC zeta enhances insulin-like growth factor 1-dependent mitogenic activity in the rat clonal beta cell line RIN 1046-38. Biochem Biophys Res Commun. 2002;290:85-90 pubmed
    ..Our data suggest that PKC zeta is involved in basal as well as IGF-1-dependent mitogenesis in RIN 1046-38 cells, while none of the PKC isoforms tested seem to be related to glucose-stimulated insulin release. ..
  45. Mancini A, Koch A, Wilms R, Tamura T. c-Cbl associates directly with the C-terminal tail of the receptor for the macrophage colony-stimulating factor, c-Fms, and down-modulates this receptor but not the viral oncogene v-Fms. J Biol Chem. 2002;277:14635-40 pubmed
    ..These data suggest that an indirect association of c-Cbl via multimeric complex induced a different signaling pathway from the pathway induced by c-Cbl direct interaction. ..
  46. Plotz G, Raedle J, Brieger A, Trojan J, Zeuzem S. N-terminus of hMLH1 confers interaction of hMutLalpha and hMutLbeta with hMutSalpha. Nucleic Acids Res. 2003;31:3217-26 pubmed
    ..We also examined another human homologue of bacterial MutL, hMutLbeta (hMLH1-hPMS1). We show that hMutLbeta interacts as efficiently with hMutSalpha as hMutLalpha, and that it predominantly binds to hMutSalpha via hMLH1 as well. ..
  47. Schumacher S, Stube E. Regulated binding of the fibrinogen-like domains of tenascin-R and tenascin-C to the neural EGF family member CALEB. J Neurochem. 2003;87:1213-23 pubmed
    ..While both CALEB-80 and CALEB-140 are expressed in the plexiform layers and the optic fiber layer of embryonic chicken retina, CALEB-140 labeling is more intense in the optic fiber layer in comparison to the inner plexiform layer. ..
  48. Bachmann M, Hennemann H, Xing P, Hoffmann I, Moroy T. The oncogenic serine/threonine kinase Pim-1 phosphorylates and inhibits the activity of Cdc25C-associated kinase 1 (C-TAK1): a novel role for Pim-1 at the G2/M cell cycle checkpoint. J Biol Chem. 2004;279:48319-28 pubmed
    ..Hence our findings directly suggest a novel role for Pim-1 as a positive regulator at the G(2)/M transition of the cell cycle. ..
  49. Zhou A, Wozniak A, Meyer Lipp K, Nietschke M, Jung H, Fendler K. Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli. J Mol Biol. 2004;343:931-42 pubmed
    ..4) Proline binding proceeds in a two-step process: low affinity (approximately 1mM) electroneutral substrate binding followed by a nearly irreversible electrogenic conformational transition. ..
  50. Richly H, Rape M, Braun S, Rumpf S, Hoege C, Jentsch S. A series of ubiquitin binding factors connects CDC48/p97 to substrate multiubiquitylation and proteasomal targeting. Cell. 2005;120:73-84 pubmed
    ..In yeast, this escort pathway guides a transcription factor from its activation in the cytosol to its final degradation and also mediates proteolysis at the endoplasmic reticulum by the ERAD pathway. ..
  51. Eissmann P, Beauchamp L, Wooters J, Tilton J, Long E, Watzl C. Molecular basis for positive and negative signaling by the natural killer cell receptor 2B4 (CD244). Blood. 2005;105:4722-9 pubmed
    ..We now show that Fyn can also associate with phosphorylated 2B4. Finally, we demonstrate that Fyn and Csk can both phosphorylate 2B4, suggesting a possible mechanism of 2B4 phosphorylation. ..
  52. Wierstra I, Alves J. Cyclin E/Cdk2, P/CAF, and E1A regulate the transactivation of the c-myc promoter by FOXM1. Biochem Biophys Res Commun. 2008;368:107-15 pubmed publisher
    ..In contrast, we now demonstrate that this LXL-motif is not required for the activation of FOXM1c by cyclin D1/Cdk4, cyclin E/Cdk and cyclin A/Cdk2 or for the repression of FOXM1c by p27. ..
  53. Ewen K, Kleser M, Bernhardt R. Adrenodoxin: the archetype of vertebrate-type [2Fe-2S] cluster ferredoxins. Biochim Biophys Acta. 2011;1814:111-25 pubmed publisher
    ..Despite their different functions, these proteins display an amazing similarity regarding their primary sequence, tertiary structure and biophysical features. ..
  54. Hoffmann C, Gaietta G, Zürn A, Adams S, Terrillon S, Ellisman M, et al. Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc. 2010;5:1666-77 pubmed publisher
    ..The labeling procedure using FlAsH-EDT? as described takes 2-3 h, depending on the number of samples to be processed. ..
  55. Boland A, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O. Crystal structure of the MID-PIWI lobe of a eukaryotic Argonaute protein. Proc Natl Acad Sci U S A. 2011;108:10466-71 pubmed publisher
    ..The structure of the QDE-2 MID-PIWI lobe provides molecular and mechanistic insight into eukaryotic AGOs and has significant implications for understanding the role of these proteins in silencing. ..
  56. Murachelli A, Ebert J, Basquin C, Le Hir H, Conti E. The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex. Nat Struct Mol Biol. 2012;19:378-86 pubmed publisher
    ..Unexpectedly, our structural analysis identified an Acinus-like motif in Pinin, another EJC-associated splicing factor. We show that Pinin physically interacts with RNPS1 and SAP18, forming an alternative ternary complex, PSAP. ..
  57. Eckl J, Scherr M, Freiburger L, Daake M, Sattler M, Richter K. Hsp90·Cdc37 Complexes with Protein Kinases Form Cooperatively with Multiple Distinct Interaction Sites. J Biol Chem. 2015;290:30843-54 pubmed publisher
  58. Rossig L, Badorff C, Holzmann Y, Zeiher A, Dimmeler S. Glycogen synthase kinase-3 couples AKT-dependent signaling to the regulation of p21Cip1 degradation. J Biol Chem. 2002;277:9684-9 pubmed
    ..001). These data indicate that GSK-3 triggers p21(Cip1) degradation. In contrast, stimulation of AKT increases p21(Cip1) via inhibitory phosphorylation of GSK-3. ..
  59. Waldmann V, Wacker J, Deichmann M. Absence of mutations in the pleckstrin homology (PH) domain of protein kinase B (PKB/Akt) in malignant melanoma. Melanoma Res. 2002;12:45-50 pubmed
    ..Thus in human melanoma PH domain mutations of PKB/Akt do not play a major role in melanoma carcinogenesis. ..
  60. Schulte A, Czudnochowski N, Barboric M, Schönichen A, Blazek D, Peterlin B, et al. Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat. J Biol Chem. 2005;280:24968-77 pubmed
    ..Our data suggest a model where HIV-1 Tat competes with Hexim1 for cyclin T1 binding, thus releasing P-TEFb from the inactive complex to stimulate the transcription of HIV-1 gene expression. ..
  61. Puccetti E, Zheng X, Brambilla D, Seshire A, Beissert T, Boehrer S, et al. The integrity of the charged pocket in the BTB/POZ domain is essential for the phenotype induced by the leukemia-associated t(11;17) fusion protein PLZF/RARalpha. Cancer Res. 2005;65:6080-8 pubmed
    ..Taken together, these data provide evidence that the dimerization capacity and the formation of a functionally charged pocket are indispensable for the PLZF/RARalpha-induced leukemogenesis. ..
  62. Yuan X, Feng W, Imhof A, Grummt I, Zhou Y. Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a. Mol Cell. 2007;27:585-95 pubmed
    ..The results reveal the mechanism underlying CSB-mediated activation of rDNA transcription and link G9a-dependent H3K9 methylation to Pol I transcription elongation through chromatin. ..
  63. Calzado M, de La Vega L, Möller A, Bowtell D, Schmitz M. An inducible autoregulatory loop between HIPK2 and Siah2 at the apex of the hypoxic response. Nat Cell Biol. 2009;11:85-91 pubmed publisher
    ..As HIPK2 has an important role as a negative regulator of gene expression, its elimination from promoter-associated repressor complexes allows the induction of a substantial fraction of hypoxia-induced genes. ..
  64. Schuldt L, Weyand S, Kefala G, Weiss M. The three-dimensional Structure of a mycobacterial DapD provides insights into DapD diversity and reveals unexpected particulars about the enzymatic mechanism. J Mol Biol. 2009;389:863-79 pubmed publisher
    ..The N-terminal domain of Mtb-DapD exhibits a unique architecture, including an interior water-filled channel, which allows access to a magnesium ion located at the 3-fold symmetry axis...
  65. Koch C, Heine A, Klebe G. Tracing the detail: how mutations affect binding modes and thermodynamic signatures of closely related aldose reductase inhibitors. J Mol Biol. 2011;406:700-12 pubmed publisher
    ..This also provides deeper insights into how single-site mutations can alter the selectivity profile of closely related ligands against a target protein. ..
  66. Murawska M, Hassler M, Renkawitz Pohl R, Ladurner A, Brehm A. Stress-induced PARP activation mediates recruitment of Drosophila Mi-2 to promote heat shock gene expression. PLoS Genet. 2011;7:e1002206 pubmed publisher
    ..We suggest that stress-induced chromatin PARylation serves to rapidly attract factors that are required for an efficient and timely transcriptional response. ..
  67. Jank T, Böhmer K, Tzivelekidis T, Schwan C, Belyi Y, Aktories K. Domain organization of Legionella effector SetA. Cell Microbiol. 2012;14:852-68 pubmed publisher
    ..Both, the localization and the glucosyltransferase domains of SetA are crucial for cellular functions...
  68. Bögershausen N, Shahrzad N, Chong J, von Kleist Retzow J, Stanga D, Li Y, et al. Recessive TRAPPC11 mutations cause a disease spectrum of limb girdle muscular dystrophy and myopathy with movement disorder and intellectual disability. Am J Hum Genet. 2013;93:181-90 pubmed publisher
  69. Eggert H, Schulz M, Fackelmayer F, Renkawitz R, Eggert M. Effects of the heterogeneous nuclear ribonucleoprotein U (hnRNP U/SAF-A) on glucocorticoid-dependent transcription in vivo. J Steroid Biochem Mol Biol. 2001;78:59-65 pubmed
    ..The relative concentrations of GR, hnRNP U and DNA-binding sites for GR are important for the effect of hnRNP U on transcription, suggesting a model where hnRNP-U acts as a storage site for intranuclear GR. ..
  70. Wu Y, Chen Z, Ullrich A. EGFR and FGFR signaling through FRS2 is subject to negative feedback control by ERK1/2. Biol Chem. 2003;384:1215-26 pubmed
    ..Our findings support a broader role of FRS2 in EGFR-controlled signaling pathways in A-431 cells and provide insight into a molecular mechanism for ligand-stimulated feedback regulation with FRS2 as a central regulatory switch point. ..
  71. Söderhäll J, Polymeropoulos E, Paulini K, Gunther E, Kühne R. Antagonist and agonist binding models of the human gonadotropin-releasing hormone receptor. Biochem Biophys Res Commun. 2005;333:568-82 pubmed
    ..Our results indicate that the binding conformation of the antagonists differs from that of the agonists. This difference can be linked to the activation or inhibition of the receptor. ..
  72. Reif S, Randelj O, Domanska G, Dian E, Krimmer T, Motz C, et al. Conserved mechanism of Oxa1 insertion into the mitochondrial inner membrane. J Mol Biol. 2005;354:520-8 pubmed
    ..We conclude that Oxa1 has the ability to accept preproteins within the membrane. ..
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