Genomes and Genes
Experts and Doctors on protein binding in Belgium
Topic: protein binding
Publications256 found, 100 shown here
- Langlet C, Langer I, Vertongen P, Gaspard N, Vanderwinden J, Robberecht P. Contribution of the carboxyl terminus of the VPAC1 receptor to agonist-induced receptor phosphorylation, internalization, and recycling. J Biol Chem. 2005;280:28034-43 pubmed..Receptor recovery was blocked by monensin, an endosome inhibitor. ..
- Piessevaux J, Lavens D, Montoye T, Wauman J, Catteeuw D, Vandekerckhove J, et al. Functional cross-modulation between SOCS proteins can stimulate cytokine signaling. J Biol Chem. 2006;281:32953-66 pubmed..Our findings point to a unique regulatory role for SOCS2, SOCS6, and SOCS7 within the SOCS family and provide an explanation for the unexpected phenotypes observed in SOCS2 and SOCS6 transgenic mice. ..
- Gloire G, Horion J, El Mjiyad N, Bex F, Chariot A, Dejardin E, et al. Promoter-dependent effect of IKKalpha on NF-kappaB/p65 DNA binding. J Biol Chem. 2007;282:21308-18 pubmed..We conclude that nuclear IKKalpha is required for p65 DNA binding in a gene-specific manner. ..
- Crèvecoeur J, Merville M, Piette J, Gloire G. Geldanamycin inhibits tyrosine phosphorylation-dependent NF-kappaB activation. Biochem Pharmacol. 2008;75:2183-91 pubmed publisher..The effect of GA on PV-induced apoptosis is discussed in the light of recent publications in the literature. ..
- D Haene B, Attanasio C, Beysen D, Dostie J, Lemire E, Bouchard P, et al. Disease-causing 7.4 kb cis-regulatory deletion disrupting conserved non-coding sequences and their interaction with the FOXL2 promotor: implications for mutation screening. PLoS Genet. 2009;5:e1000522 pubmed publisher..4 kb deleted fragment. Overall, this study revealed the smallest distant deletion causing monogenic disease and impacts upon the concept of mutation screening in human disease and developmental disorders in particular...
- Henry X, Amoroso A, Coyette J, Joris B. Interaction of ceftobiprole with the low-affinity PBP 5 of Enterococcus faecium. Antimicrob Agents Chemother. 2010;54:953-5 pubmed publisher..Our results show that ceftobiprole binds to unmutated PBP 5fm to form a stable acyl-enzyme and that ceftobiprole is able to efficiently kill a penicillin-resistant Enterococcus faecium strain that produces this protein. ..
- Janssens V, Zwaenepoel K, Rosse C, Petit M, Goris J, Parker P. PP2A binds to the LIM domains of lipoma-preferred partner through its PR130/B″ subunit to regulate cell adhesion and migration. J Cell Sci. 2016;129:1605-18 pubmed publisher..Our data highlight the importance of specific, locally recruited PP2A complexes in cell adhesion and migration dynamics. ..
- Laeremans H, Demaegdt H, De Backer J, Le M, Kersemans V, Michotte Y, et al. Metal ion modulation of cystinyl aminopeptidase. Biochem J. 2005;390:351-7 pubmed..These findings support the concept that high-affinity [125I]angiotensin IV binding, previously referred to as 'AT4 receptor binding', only occurs for the cystinyl aminopeptidase apoenzyme. ..
- Sercu S, Lambeir A, Steenackers E, El Ghalbzouri A, Geentjens K, Sasaki T, et al. ECM1 interacts with fibulin-3 and the beta 3 chain of laminin 332 through its serum albumin subdomain-like 2 domain. Matrix Biol. 2009;28:160-9 pubmed publisher..Hence, disruption of the ECM1 function may cause the failure of multi-communication among the surrounding skin interstitial molecules, as seen in lipoid proteinosis pathology...
- Lambeir A, Proost P, Durinx C, Bal G, Senten K, Augustyns K, et al. Kinetic investigation of chemokine truncation by CD26/dipeptidyl peptidase IV reveals a striking selectivity within the chemokine family. J Biol Chem. 2001;276:29839-45 pubmed..The data not only provide insight into the selectivity of the enzyme for specific chemokines, but they also contribute to the general understanding of CD26/dipeptidyl peptidase IV secondary substrate specificity. ..
- Fransen M, Wylin T, Brees C, Mannaerts G, Van Veldhoven P. Human pex19p binds peroxisomal integral membrane proteins at regions distinct from their sorting sequences. Mol Cell Biol. 2001;21:4413-24 pubmed
- Bultinck J, Lievens S, Tavernier J. Protein-protein interactions: network analysis and applications in drug discovery. Curr Pharm Des. 2012;18:4619-29 pubmed
- Grootjans J, Reekmans G, Ceulemans H, David G. Syntenin-syndecan binding requires syndecan-synteny and the co-operation of both PDZ domains of syntenin. J Biol Chem. 2000;275:19933-41 pubmed..In general, an intramolecular combination of PDZ domains that are weak, taken individually, would appear to be designed to detect rather than drive the formation of specific molecular assemblies. ..
- Hertveldt K, Dechassa M, Robben J, Volckaert G. Identification of Gal80p-interacting proteins by Saccharomyces cerevisiae whole genome phage display. Gene. 2003;307:141-9 pubmed..Interactions were confirmed by ELISA. These results demonstrate the accessibility of the S. cerevisiae genome to display technology for protein-protein interaction screening. ..
- Leimanis S, Hoyez N, Hubert S, Laschet M, Sauvage E, Brasseur R, et al. PBP5 complementation of a PBP3 deficiency in Enterococcus hirae. J Bacteriol. 2006;188:6298-307 pubmed..The P197-N209 segment (site E) in one of these domains seemed to be particularly important, as single and double mutations reduced or almost completely abolished, respectively, the action of PBP5. ..
- Vanheusden V, Munier Lehmann H, Froeyen M, Busson R, Rozenski J, Herdewijn P, et al. Discovery of bicyclic thymidine analogues as selective and high-affinity inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase. J Med Chem. 2004;47:6187-94 pubmed..Hereby, we report on the discovery of a novel class of bicyclic nucleosides (10 and 11) and one dinucleoside (12), belonging to the most selective inhibitors of TMPKmt discovered so far. ..
- Nelissen H, De Groeve S, Fleury D, Neyt P, Bruno L, Bitonti M, et al. Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation. Proc Natl Acad Sci U S A. 2010;107:1678-83 pubmed publisher..Thus, although the structure of Elongator and its substrate are conserved, target gene selection has diverged, showing that auxin signaling and influx are under chromatin control. ..
- Mvumbi L, Stalmans W. High-affinity binding of glycogen-synthase phosphatase to glycogen particles in the liver. Role of glycogen in the inhibition of synthase phosphatase by phosphorylase a. Biochem J. 1987;246:367-74 pubmed..25 mg/ml, and virtually complete at 1 mg/ml. These findings explain long-standing observations that in fasted animals the liver contains appreciable amounts of both synthase and phosphorylase in the active form. ..
- Ceulemans H, Vulsteke V, De Maeyer M, Tatchell K, Stalmans W, Bollen M. Binding of the concave surface of the Sds22 superhelix to the alpha 4/alpha 5/alpha 6-triangle of protein phosphatase-1. J Biol Chem. 2002;277:47331-7 pubmed..Our data also show that well known regulatory binding sites of PP1, such as the RVXF-binding channel, the beta12/beta13-loop, and the acidic groove, are not essential for the interaction with Sds22. ..
- Gils A, Declerck P. The structural basis for the pathophysiological relevance of PAI-I in cardiovascular diseases and the development of potential PAI-I inhibitors. Thromb Haemost. 2004;91:425-37 pubmed
- Bentaya S, Ghogomu S, Vanhomwegen J, Van Campenhout C, Thelie A, Dhainaut M, et al. The RNA-binding protein XSeb4R regulates maternal Sox3 at the posttranscriptional level during maternal-zygotic transition in Xenopus. Dev Biol. 2012;363:362-72 pubmed publisher..K., an ectodermal marker. Overall, our data suggest XSeb4R as a novel player in gene expression regulation, acting at the posttranscriptional level during ectoderm specification in Xenopus. ..
- Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W. Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose. J Mol Biol. 2008;377:378-85 pubmed publisher..Unravelling the exact position of sucrose in plant cell-wall invertases is a necessary step towards the rational design of superior invertases to further increase crop yield and biomass production. ..
- Wawrzak D, Metioui M, Willems E, Hendrickx M, De Genst E, Leyns L. Wnt3a binds to several sFRPs in the nanomolar range. Biochem Biophys Res Commun. 2007;357:1119-23 pubmed..These results provide the first measurement of binding affinity of sFRPs for a Wnt, which together with the measurement of antagonistic activity of sFRPs could help understand how sFRPs regulate Wnt signaling. ..
- Brozzi F, Gerlo S, Grieco F, Juusola M, Balhuizen A, Lievens S, et al. Ubiquitin D Regulates IRE1?/c-Jun N-terminal Kinase (JNK) Protein-dependent Apoptosis in Pancreatic Beta Cells. J Biol Chem. 2016;291:12040-56 pubmed publisher..Our data suggest that UBD provides a negative feedback on cytokine-induced activation of the IRE1?/JNK pro-apoptotic pathway in cytokine-exposed beta cells. ..
- Peeters T, Louwet W, Geladé R, Nauwelaers D, Thevelein J, Versele M. Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast. Proc Natl Acad Sci U S A. 2006;103:13034-9 pubmed..Importantly, we show that Krh1/2 also enhance the association between mouse R and C subunits, suggesting that Krh control of PKA has been evolutionarily conserved. ..
- Arza B, De Maeyer M, Felez J, Collen D, Lijnen H. Critical role of glutamic acid 202 in the enzymatic activity of stromelysin-1 (MMP-3). Eur J Biochem. 2001;268:826-31 pubmed..Taken together, these data suggest that Glu202 plays a crucial role in the enzymatic activity of MMP-3. ..
- El Mjiyad N, Bontems S, Gloire G, Horion J, Vandevenne P, Dejardin E, et al. Varicella-zoster virus modulates NF-kappaB recruitment on selected cellular promoters. J Virol. 2007;81:13092-104 pubmed..Targeting NF-kappaB activation, which plays a central role in innate and adaptive immune responses, leads to obvious advantages for the virus, particularly in melanocytes, which are a site of viral replication in the skin. ..
- Kwasigroch J, Wintjens R, Gilis D, Rooman M. SODa: an Mn/Fe superoxide dismutase prediction and design server. BMC Bioinformatics. 2008;9:257 pubmed publisher..SODa is shown to outperform and to be more discriminative than traditional techniques based on pairwise sequence alignments. Moreover, the fact that it proposes selected mutations makes it a valuable tool for rational protein design. ..
- Laenen K, Haegeman G, Vanhoenacker P. Structure of the human 5-HT7 receptor gene and characterization of its promoter region. Gene. 2007;391:252-63 pubmed..Taken together these results support the essential role of Sp factors in regulating 5-HT(7) promoter activity. ..
- Meersman F, Bowron D, Soper A, Koch M. An X-ray and neutron scattering study of the equilibrium between trimethylamine N-oxide and urea in aqueous solution. Phys Chem Chem Phys. 2011;13:13765-71 pubmed publisher..14 M(-1), which is of the same order as the experimental urea-protein binding constants per site reported in the literature. This confirms that the two osmolytes act independently at least in the physiological range...
- Andrei G, Fiten P, Froeyen M, De Clercq E, Opdenakker G, Snoeck R. DNA polymerase mutations in drug-resistant herpes simplex virus mutants determine in vivo neurovirulence and drug-enzyme interactions. Antivir Ther. 2007;12:719-32 pubmed..It appears that drug-resistant mutants arising under the pressure of HPMP derivatives have the lowest levels of neurovirulence...
- Cho H, Ryu H, Rho S, Hill K, Smith S, Audenaert D, et al. A secreted peptide acts on BIN2-mediated phosphorylation of ARFs to potentiate auxin response during lateral root development. Nat Cell Biol. 2014;16:66-76 pubmed publisher..In summary, this study delineates a TDIF-TDR-BIN2 signalling cascade that controls regulation of ARF and AUX/IAA interaction independent of auxin perception during lateral root development...
- Lijnen H, Van Hoef B, Beelen V, Collen D. Characterization of the murine plasma fibrinolytic system. Eur J Biochem. 1994;224:863-71 pubmed
- Roskams T, Libbrecht L, van Damme B, Desmet V. Fas and Fas ligand: strong co-expression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells?. J Pathol. 2000;191:150-3 pubmed..Within the tumour, the Fas-Fas-L apoptosis pathway seems to be little involved. ..
- Constantinescu S, Keren T, Russ W, Ubarretxena Belandia I, Malka Y, Kubatzky K, et al. The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins. J Biol Chem. 2003;278:43755-63 pubmed..As unliganded EpoR forms TM-dependent but inactive homodimers, we propose that the EpoR can be activated to different extents by homodimeric gp55 proteins, depending on the conformation of the gp55 protein dimer in the TM region. ..
- Roy N, Van Eynde A, Beke L, Nuytten M, Bollen M. The transcriptional repression by NIPP1 is mediated by Polycomb group proteins. Biochim Biophys Acta. 2007;1769:541-5 pubmed..NIPP1 is present in a complex with EED and EZH2 in vivo and has distinct binding sites for these proteins. These data disclose an essential role for the PRC2 complex in the transcriptional repression by NIPP1. ..
- Grupping A, Cnop M, Van Schravendijk C, Hannaert J, van Berkel T, Pipeleers D. Low density lipoprotein binding and uptake by human and rat islet beta cells. Endocrinology. 1997;138:4064-8 pubmed..In conclusion, human and rat islet beta cells express LDL receptors that can internalize the lipoprotein. This pathway should be examined for its potential role in (dys)regulating pancreatic beta cell functions. ..
- Nikko E, Andre B. Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase. Eukaryot Cell. 2007;6:1266-77 pubmed..Our data indicate that Bro1 interacts with several ESCRT components and contributes via its PRD to associating ubiquitinating and deubiquitinating enzymes with the MVB sorting machinery. ..
- Blanpain C, Doranz B, Bondue A, Govaerts C, De Leener A, Vassart G, et al. The core domain of chemokines binds CCR5 extracellular domains while their amino terminus interacts with the transmembrane helix bundle. J Biol Chem. 2003;278:5179-87 pubmed
- Verbelen C, Dupres V, Raze D, Bompard C, Locht C, Dufrêne Y. Interaction of the mycobacterial heparin-binding hemagglutinin with actin, as evidenced by single-molecule force spectroscopy. J Bacteriol. 2008;190:7614-20 pubmed publisher..Taken together, our data indicate that HBHA is able to specifically bind actin, via both its N-terminal and C-terminal domains, strongly suggesting a role of the HBHA-actin interaction in the pathogenesis of mycobacterial diseases. ..
- Georis I, Feller A, Vierendeels F, Dubois E. The yeast GATA factor Gat1 occupies a central position in nitrogen catabolite repression-sensitive gene activation. Mol Cell Biol. 2009;29:3803-15 pubmed publisher..Dal80-dependent repression of NCR-sensitive gene expression occurred at three possible levels: Dal80 represses GAT1 expression, it competes with Gat1 for binding, and it directly represses NCR gene transcription. ..
- Costes B, Ruiz Argüello M, Bryant N, Alcami A, Vanderplasschen A. Both soluble and membrane-anchored forms of Felid herpesvirus 1 glycoprotein G function as a broad-spectrum chemokine-binding protein. J Gen Virol. 2005;86:3209-14 pubmed..Altogether, these data demonstrate that FeHV-1 gG is a new member of the vCKBP-4 family. Moreover, this study is the first to demonstrate that gG expressed at the surface of FeHV-1-infected cells can also bind chemokines. ..
- Beaudry J, Pierreux C, Hayhurst G, Plumb Rudewiez N, Weiss M, Rousseau G, et al. Threshold levels of hepatocyte nuclear factor 6 (HNF-6) acting in synergy with HNF-4 and PGC-1alpha are required for time-specific gene expression during liver development. Mol Cell Biol. 2006;26:6037-46 pubmed..Our observations on the regulation of g6pc by HNF-6 provide a model whereby synergism, interdependency, and threshold concentrations of LETFs and coactivators determine time-specific expression of genes during liver development. ..
- Balzarini J, Pelemans H, Aquaro S, Perno C, Witvrouw M, Schols D, et al. Highly favorable antiviral activity and resistance profile of the novel thiocarboxanilide pentenyloxy ether derivatives UC-781 and UC-82 as inhibitors of human immunodeficiency virus type 1 replication. Mol Pharmacol. 1996;50:394-401 pubmed
- Van Driessche B, Coddens S, Van Mullem V, Vandenhaute J. Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae. FEBS Lett. 2005;579:5318-24 pubmed..Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2. These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation. ..
- Auwerx J, François K, Covens K, Van Laethem K, Balzarini J. Glycan deletions in the HIV-1 gp120 V1/V2 domain compromise viral infectivity, sensitize the mutant virus strains to carbohydrate-binding agents and represent a specific target for therapeutic intervention. Virology. 2008;382:10-9 pubmed publisher..The gp120 V1/V2 loop glycans of HIV-1 should therefore be considered as a hot spot and novel target for specific therapeutic drug intervention. ..
- Ulrichts P, Lemmens I, Lavens D, Beyaert R, Tavernier J. MAPPIT (mammalian protein-protein interaction trap) analysis of early steps in toll-like receptor signalling. Methods Mol Biol. 2009;517:133-44 pubmed publisher..In the subsequent protocol, we describe the use of MAPPIT to study early events in Toll-like receptor (TLR) signalling. We here demonstrate a "signalling interaction cascade" from TLR4 to IRAK-1. ..
- Fransen M, Vastiau I, Brees C, Brys V, Mannaerts G, Van Veldhoven P. Potential role for Pex19p in assembly of PTS-receptor docking complexes. J Biol Chem. 2004;279:12615-24 pubmed..Importantly, we also found that amino acid substitutions resulting in a strongly reduced binding affinity for Pex13p affect the peroxisomal localization of Pex14p. ..
- Zimmermann P. The prevalence and significance of PDZ domain-phosphoinositide interactions. Biochim Biophys Acta. 2006;1761:947-56 pubmed..In addition, other lower-affinity PDZ domain/PIPs interactions are documented. Here, we summarize and discuss the present knowledge about the occurrence, the biochemistry and the biology of PDZ domain-lipid interactions. ..
- Vanholder R, Glorieux G, Lameire N. New insights in uremic toxicity. Contrib Nephrol. 2005;149:315-24 pubmed..Especially difficult to remove compounds, such as protein bound and larger molecules, seem to play a role. New strategies enhancing their removal might be highly useful. ..
- Le Roy K, Lammens W, Van Laere A, Van den Ende W. Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase. New Phytol. 2008;178:572-80 pubmed publisher..As single amino acid substitutions can influence the donor substrate specificity of FEHs, it is predicted that plant invertases and FEHs may have diversified by introduction of a very limited number of mutations in the common ancestor. ..
- Dieriks B, Van Oostveldt P. Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. Int J Mol Med. 2012;29:1031-8 pubmed publisher..Supported by in silico data, our observations suggest that CypB interacts with these proteins and is involved in ribosome biogenesis and RNA transcription. ..
- Janssens B, Goossens S, Staes K, Gilbert B, van Hengel J, Colpaert C, et al. alphaT-catenin: a novel tissue-specific beta-catenin-binding protein mediating strong cell-cell adhesion. J Cell Sci. 2001;114:3177-88 pubmed..We propose that alphaT-catenin is necessary for the formation of stretch-resistant cell-cell adhesion complexes, in particular, muscle cells. ..
- Christiaens B, Grooten J, Reusens M, Joliot A, Goethals M, Vandekerckhove J, et al. Membrane interaction and cellular internalization of penetratin peptides. Eur J Biochem. 2004;271:1187-97 pubmed..None of the penetratin variants was haemolytic or cytotoxic. ..
- De Meester I, Vanham G, Kestens L, Vanhoof G, Bosmans E, Gigase P, et al. Binding of adenosine deaminase to the lymphocyte surface via CD26. Eur J Immunol. 1994;24:566-70 pubmed..Neither soluble nor immobilized ADA was able to down-modulate CD26 on the lymphocyte surface. Our data thus confirm the identity between ADAbp and CD26 and identify some epitopes, crucial in the binding of ADA to CD26. ..
- Fett T, Zecchinon L, Vanden Bergh P, Desmecht D. Mannheimia haemolytica leukotoxin-induced cytolysis of caprine (Capra hircus) leukocytes is mediated by the CD18 subunit of beta2-integrins. Microb Pathog. 2008;45:337-42 pubmed publisher..Taken together, these results indicate that CD18 on caprine leukocytes serves as a receptor for Lkt. ..
- Renard P, Ernest I, Houbion A, Art M, Le Calvez H, Raes M, et al. Development of a sensitive multi-well colorimetric assay for active NFkappaB. Nucleic Acids Res. 2001;29:E21 pubmed..This assay is easy to use, non-radioactive, highly reproducible, specific for NFkappaB, more sensitive than regular radioactive gel shift and very convenient for high throughput screening. ..
- Mircescu H, Steuve S, Savonet V, Degraef C, Mellor H, Dumont J, et al. Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP. Eur J Biochem. 2002;269:6241-9 pubmed
- Roosbeek S, Caster H, Liu Q, Berne P, Duverger N, Christiaens B, et al. Expression and activity of the nucleotide-binding domains of the human ABCA1 transporter. Protein Expr Purif. 2004;35:102-10 pubmed..This approach should prove useful for the study of the structural and functional properties of the NBDs and other domains of the ABC transporters...
- Burvenich I, Schoonooghe S, Vervoort L, Dumolyn C, Coene E, Vanwalleghem L, et al. Monoclonal antibody 14C5 targets integrin alphavbeta5. Mol Cancer Ther. 2008;7:3771-9 pubmed publisher..We show for the first time that mAb 14C5 binds the vascular integrin alphavbeta5, suggesting that mAb 14C5 can be used as a screening agent to select colon and lung cancer patients that are eligible for anti-alphavbeta5-based therapies. ..
- Hadži S, Garcia Pino A, Haesaerts S, Jurenas D, Gerdes K, Lah J, et al. Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a ?-strand sliding mechanism. Nucleic Acids Res. 2017;45:4972-4983 pubmed publisher..This interaction displaces helix ?3 of VcHigB2 and at the same time induces a one-residue shift in the register of ?-strand ?3, thereby flipping the catalytically important Arg64 out of the active site. ..
- Brohée S, van Helden J. Evaluation of clustering algorithms for protein-protein interaction networks. BMC Bioinformatics. 2006;7:488 pubmed..The other two algorithms are clearly weaker under most conditions. The analysis of high-throughput data supports the superiority of MCL for the extraction of complexes from interaction networks. ..
- Lesage B, Beullens M, Pedelini L, Garcia Gimeno M, Waelkens E, Sanz P, et al. A complex of catalytically inactive protein phosphatase-1 sandwiched between Sds22 and inhibitor-3. Biochemistry. 2007;46:8909-19 pubmed..Therefore, our studies show that a pool of PP1 is complexly controlled by both Sds22 and I3. ..
- Tinton S, Schepens B, Bruynooghe Y, Beyaert R, Cornelis S. Regulation of the cell-cycle-dependent internal ribosome entry site of the PITSLRE protein kinase: roles of Unr (upstream of N-ras) protein and phosphorylated translation initiation factor eIF-2alpha. Biochem J. 2005;385:155-63 pubmed
- Urizar E, Claeysen S, Deupi X, Govaerts C, Costagliola S, Vassart G, et al. An activation switch in the rhodopsin family of G protein-coupled receptors: the thyrotropin receptor. J Biol Chem. 2005;280:17135-41 pubmed..As residues Leu-2.46, Asp-2.50, and Asn-7.49 are strongly conserved, this molecular mechanism of TSHr activation can be extended to other members of the rhodopsin-like family of G protein-coupled receptors. ..
- Salmon D, Geuskens M, Hanocq F, Hanocq Quertier J, Nolan D, Ruben L, et al. A novel heterodimeric transferrin receptor encoded by a pair of VSG expression site-associated genes in T. brucei. Cell. 1994;78:75-86 pubmed..Both pESAG-7/6 and Tf are internalized via coated pits and vesicles. These observations suggest a novel mode of Tf binding and uptake in trypanosomes...
- Vreuls C, Filee P, Van Melckebeke H, Aerts T, De Deyn P, Llabres G, et al. Guanidinium chloride denaturation of the dimeric Bacillus licheniformis BlaI repressor highlights an independent domain unfolding pathway. Biochem J. 2004;384:179-90 pubmed..These results contribute to the characterization of the BlaI dimerization domain (i.e. CTD) involved in the induction process. ..
- Briers Y, Volckaert G, Cornelissen A, Lagaert S, Michiels C, Hertveldt K, et al. Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages phiKZ and EL. Mol Microbiol. 2007;65:1334-44 pubmed publisher..Both KZ144 and EL188 appear to be a natural chimeric enzyme, originating from a recombination of a cell wall-binding domain encoded by a Bacillus or Clostridium species and a catalytic domain of an unknown ancestor...
- Azarkan M, Martinez Rodriguez S, Buts L, Baeyens Volant D, Garcia Pino A. The plasticity of the ?-trefoil fold constitutes an evolutionary platform for protease inhibition. J Biol Chem. 2011;286:43726-34 pubmed publisher..Based on sequence and structural analysis, we hypothesize that the inherent plasticity of the ?-trefoil fold is paramount in the functional evolution of this family toward multiple protease inhibition. ..
- Hardt K, Joris B, Lepage S, Brasseur R, Lampen J, Fr re J, et al. The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle. Mol Microbiol. 1997;23:935-44 pubmed..Signal emission in the cytosol by the intracellular 189-amino-acid-residue Y134-K322 loop that connects TM3 and TM4, may proceed via the activation of a putative metallopeptidase...
- Cauwenberghs N, Vanhoorelbeke K, Vauterin S, Westra D, Romo G, Huizinga E, et al. Epitope mapping of inhibitory antibodies against platelet glycoprotein Ibalpha reveals interaction between the leucine-rich repeat N-terminal and C-terminal flanking domains of glycoprotein Ibalpha. Blood. 2001;98:652-60 pubmed..Moreover, further support is provided for the existence of an intramolecular interaction between the N-terminal flanking (aa 1-81) and C-terminal flanking (aa 201-268) regions. (Blood. 2001;98:652-660) ..
- Bollen M, Peti W, Ragusa M, Beullens M. The extended PP1 toolkit: designed to create specificity. Trends Biochem Sci. 2010;35:450-8 pubmed publisher..These novel insights offer perspectives for the therapeutic targeting of PP1 by interfering with the binding of PIPs or substrates. ..
- Pauwels L, Goossens A. The JAZ proteins: a crucial interface in the jasmonate signaling cascade. Plant Cell. 2011;23:3089-100 pubmed publisher..Overall, these contemporary findings underscore the value of protein-protein interaction studies to acquire fundamental insight into molecular signaling pathways. ..
- Tafforeau L, Chantier T, Pradezynski F, Pellet J, Mangeot P, Vidalain P, et al. Generation and comprehensive analysis of an influenza virus polymerase cellular interaction network. J Virol. 2011;85:13010-8 pubmed publisher..Conversely, we identified PRKRA, which acts as an inhibitor of the viral polymerase transcriptional activity and thus is required for the cellular antiviral response. ..
- van den Ameele J, Tiberi L, Bondue A, Paulissen C, Herpoel A, Iacovino M, et al. Eomesodermin induces Mesp1 expression and cardiac differentiation from embryonic stem cells in the absence of Activin. EMBO Rep. 2012;13:355-62 pubmed publisher..These results place Eomes upstream of the Mesp1-dependent programme of cardiogenesis, and at the intersection of mesodermal and endodermal specification, depending on the levels of Activin/Nodal signalling. ..
- Viaene L, Annaert P, de Loor H, Poesen R, Evenepoel P, Meijers B. Albumin is the main plasma binding protein for indoxyl sulfate and p-cresyl sulfate. Biopharm Drug Dispos. 2013;34:165-75 pubmed publisher..The high affinity binding site accounts for at least 90% of overall binding. Competition for this binding site could be used to augment free solute concentrations during dialysis, thus improving epuration. ..
- Ding H, Descheemaeker K, Marynen P, Nelles L, Carvalho T, Carmo Fonseca M, et al. Characterization of a helicase-like transcription factor involved in the expression of the human plasminogen activator inhibitor-1 gene. DNA Cell Biol. 1996;15:429-42 pubmed..Mutation of the PAI-1 B Box led to an eight-fold reduction of basal PAI-1-LUC expression in these cell lines, but did not affect the four- to six-fold induction by phorbol esters. ..
- Klarskov K, Van Driessche G, Backers K, Dumortier C, Meyer T, Tollin G, et al. Ligand binding and covalent structure of an oxygen-binding heme protein from Rhodobacter sphaeroides, a representative of a new structural family of c-type cytochromes. Biochemistry. 1998;37:5995-6002 pubmed publisher..There is localized positive charge (+3) at the site of reduction of SHP, although the overall protein charge is -2. This may account in part for the ability of SHP to bind anions...
- Thielemans L, Depoortere I, Vanden Broeck J, Peeters T. The motilin pharmacophore in CHO cells expressing the human motilin receptor. Biochem Biophys Res Commun. 2002;293:1223-7 pubmed..Conservation of the motilin pharmacophore in evolution indicates an important physiological role of motilin. ..
- Costes B, Thirion M, Dewals B, Mast J, Ackermann M, Markine Goriaynoff N, et al. Felid herpesvirus 1 glycoprotein G is a structural protein that mediates the binding of chemokines on the viral envelope. Microbes Infect. 2006;8:2657-67 pubmed..In the light of these results, putative roles of gG in alphaherpesvirus infections are discussed, and an evolutionary scenario is proposed to explain the structural versus non-structural property of gG amongst alphaherpesviruses. ..
- Le Roy K, Lammens W, Verhaest M, De Coninck B, Rabijns A, Van Laere A, et al. Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase. Plant Physiol. 2007;145:616-25 pubmed..The presence or absence of such an Asp-239 homolog is proposed as a reliable determinant to discriminate between real invertases and defective invertases/FEHs. ..
- Missiaen L, Van Acker K, Parys J, De Smedt H, Van Baelen K, Weidema A, et al. Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store. J Biol Chem. 2001;276:39161-70 pubmed..We conclude that the Pmr1-induced Ca(2+) store, which probably corresponds to the Golgi compartment, can play a crucial role in setting up baseline Ca(2+) spiking. ..
- Wiame E, Duquenne A, Delpierre G, van Schaftingen E. Identification of enzymes acting on alpha-glycated amino acids in Bacillus subtilis. FEBS Lett. 2004;577:469-72 pubmed..These enzymes are therefore involved in the metabolism of alpha-glycated amino acids. ..
- Evens N, Vandeputte C, Muccioli G, Lambert D, Baekelandt V, Verbruggen A, et al. Synthesis, in vitro and in vivo evaluation of fluorine-18 labelled FE-GW405833 as a PET tracer for type 2 cannabinoid receptor imaging. Bioorg Med Chem. 2011;19:4499-505 pubmed publisher..Optimized derivatives of GW405833 that are less susceptible to metabolism will need to be developed in order to provide a useful tracer for CB?R quantification with PET. ..
- Volkov A, van Nuland N. Electron transfer interactome of cytochrome C. PLoS Comput Biol. 2012;8:e1002807 pubmed publisher..Further, we show that the functional epitope size correlates with the extent of dynamics in the Cc complexes and thus can be used as a diagnostic tool for protein mobility. ..
- Bultynck G, Rossi D, Callewaert G, Missiaen L, Sorrentino V, Parys J, et al. The conserved sites for the FK506-binding proteins in ryanodine receptors and inositol 1,4,5-trisphosphate receptors are structurally and functionally different. J Biol Chem. 2001;276:47715-24 pubmed..Structural differences in the FKBP-binding site of RyRs and IP(3)Rs may contribute to the occurrence of a stable interaction between RyR isoforms and FKBPs and to the absence of such interaction with IP(3)Rs. ..
- Parker A, Van de Weyer I, Laus M, Verhasselt P, Luyten W. Identification of a human homologue of the Schizosaccharomyces pombe rad17+ checkpoint gene. J Biol Chem. 1998;273:18340-6 pubmed..We demonstrate a physical interaction between Hrad17 and Hrad1 but no interaction with Hrad9. ..