Experts and Doctors on polyacrylamide gel electrophoresis in Czech Republic
Locale: Czech Republic
Topic: polyacrylamide gel electrophoresis
Tylichova M, Briozzo P, Kopecny D, Ferrero J, Morera S, Joly N, et al
. Purification, crystallization and preliminary crystallographic study of a recombinant plant aminoaldehyde dehydrogenase from Pisum sativum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:88-90 pubmed publisher
..Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 86.4, b = 216.6, c = 205.4 A, beta = 98.1 degrees. Molecular replacement was performed and led to the identification of six dimers per asymmetric unit. ..
Komenda J, Reisinger V, Müller B, Dobakova M, Granvogl B, Eichacker L. Accumulation of the D2 protein is a key regulatory step for assembly of the photosystem II reaction center complex in Synechocystis PCC 6803. J Biol Chem. 2004;279:48620-9 pubmed
..We conclude that expression of the psbEFLJ operon is a prerequisite for D2 accumulation that is the key regulatory step for D1 accumulation and consecutive assembly of the PSII reaction center complex. ..
Bezouska K, Nepovim A, Horvath O, Pospisil M, Hamann J, Feizi T. CD 69 antigen of human lymphocytes is a calcium-dependent carbohydrate-binding protein. Biochem Biophys Res Commun. 1995;208:68-74 pubmed
..Thus, the tight association with calcium and high affinities for carbohydrate binding appear to be features of at least two members of the C-type lectin group V. ..
Kuchar M, Fajkus J. Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants. FEBS Lett. 2004;578:311-5 pubmed
..In humans, the TRF1-complex recruits hPot1 to telomeres by protein-protein interactions where it is involved in telomere length regulation. Possibly, AtTRB1 has a similar role in recruiting AtPot1. ..
Komenda J, Barker M, Kuviková S, de Vries R, Mullineaux C, Tichy M, et al
. The FtsH protease slr0228 is important for quality control of photosystem II in the thylakoid membrane of Synechocystis sp. PCC 6803. J Biol Chem. 2006;281:1145-51 pubmed
..Overall, our results show that FtsH (slr0228) plays an important role in controlling the removal of PSII subunits from the thylakoid membrane and is not restricted to selective D1 turnover. ..
Novakova Z, Man P, Novak P, Hozak P, Hodny Z. Separation of nuclear protein complexes by blue native polyacrylamide gel electrophoresis. Electrophoresis. 2006;27:1277-87 pubmed
..Moreover, practical advice for sample preparation is provided. ..
Wsol V, Szotakova B, Martin H, Maser E. Aldo-keto reductases (AKR) from the AKR1C subfamily catalyze the carbonyl reduction of the novel anticancer drug oracin in man. Toxicology. 2007;238:111-8 pubmed
..Detailed knowledge about all enzymes involved in oracin detoxification may help to improve an anticancer regimen by co-application of respective inhibitors. ..
Sterba J, Vancov M, Rudenko N, Golovchenko M, Tremblay T, Kelly J, et al
. Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. J Bacteriol. 2008;190:2619-23 pubmed publisher
..We suggest that glycoproteins present on the surface of B. burgdorferi may be tightly bound culture medium glycoproteins...
Mozgová I, Schrumpfová P, Hofr C, Fajkus J. Functional characterization of domains in AtTRB1, a putative telomere-binding protein in Arabidopsis thaliana. Phytochemistry. 2008;69:1814-9 pubmed publisher
..We show that preferential interaction of AtTRB1 with double-stranded telomeric DNA is mediated by the Myb domain, while the H1/5 domain is involved in non-specific DNA-protein interaction and in the multimerization of AtTRB1. ..