Genomes and Genes
Experts and Doctors on nucleocytoplasmic transport proteins in Germany
Topic: nucleocytoplasmic transport proteins
- Kogel D, Peters M, König H, Hashemi S, Bui N, Arolt V, et al. S100B potently activates p65/c-Rel transcriptional complexes in hippocampal neurons: Clinical implications for the role of S100B in excitotoxic brain injury. Neuroscience. 2004;127:913-20 pubmed..Our data suggest that S100B secreted during the glial response to brain injury potently activates p65/c-Rel in a RAGE-dependent manner and may exert neuroprotective and neuroregenerative effects in psychiatric disorders. ..
- Schmidt U, Richter K, Berger A, Lichter P. In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains. J Cell Biol. 2006;172:373-81 pubmed..These results suggest that a fraction of RNA, which remains in the nucleus for several hours despite its association with splicing and export proteins, accumulates in speckles because of an ATP-dependent mechanism...
- Fischer T, Rodriguez Navarro S, Pereira G, Rácz A, Schiebel E, Hurt E. Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat Cell Biol. 2004;6:840-8 pubmed..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery. ..
- Braun I, Herold A, Rode M, Conti E, Izaurralde E. Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export. J Biol Chem. 2001;276:20536-43 pubmed..Our data indicate that formation of TAP/p15 heterodimers is required for TAP-mediated export of mRNA and show that the LRR domain of TAP plays an essential role in this process...
- Herold A, Suyama M, Rodrigues J, Braun I, Kutay U, Carmo Fonseca M, et al. TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture. Mol Cell Biol. 2000;20:8996-9008 pubmed..Both human p15 homologues bind TAP, NXF2, and NXF3. Together, our results indicate that the TAP-p15 mRNA export pathway has diversified in higher eukaryotes compared to yeast, perhaps reflecting a greater substrate complexity. ..
- Liker E, Fernandez E, Izaurralde E, Conti E. The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain. EMBO J. 2000;19:5587-98 pubmed..Our in vitro and in vivo functional studies using structure-based mutants suggest that a phylogenetically conserved surface of the LRR domain of TAP may have different roles in the export of viral and cellular RNAs. ..
- Frey S, Gorlich D. A saturated FG-repeat hydrogel can reproduce the permeability properties of nuclear pore complexes. Cell. 2007;130:512-23 pubmed..Intragel diffusion of the importin beta-cargo complex occurred rapidly enough to traverse an NPC within approximately 12 ms. We extend the "selective phase model" to explain these effects. ..
- Wohlwend D, Strasser A, Dickmanns A, Ficner R. Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus. J Mol Biol. 2007;374:1129-38 pubmed
- von Moeller H, Basquin C, Conti E. The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner. Nat Struct Mol Biol. 2009;16:247-54 pubmed publisher..More generally, they provide a paradigm for how binding of regulators can specifically inhibit DEAD-box proteins. ..
- Strasser K, Hurt E. Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. EMBO J. 2000;19:410-20 pubmed..Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes. ..
- Kruse C, Willkomm D, Grünweller A, Vollbrandt T, Sommer S, Busch S, et al. Export and transport of tRNA are coupled to a multi-protein complex. Biochem J. 2000;346 Pt 1:107-15 pubmed
- Katahira J, Strasser K, Podtelejnikov A, Mann M, Jung J, Hurt E. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J. 1999;18:2593-609 pubmed..Thus, the human TAP-p15 complex can functionally replace the Mex67p-Mtr2p complex in yeast and thus performs a conserved role in nuclear mRNA export...
- Herrmann M, Neuberth N, Wissler J, Perez J, Gradl D, Naber A. Near-field optical study of protein transport kinetics at a single nuclear pore. Nano Lett. 2009;9:3330-6 pubmed publisher..NSOM-FCS may help to understand the facilitated translocation in more detail and offers a new way to study single molecule mobility on a nanoscale...
- Santos Rosa H, Moreno H, Simos G, Segref A, Fahrenkrog B, Pante N, et al. Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol. 1998;18:6826-38 pubmed..Thus, Mex67p and Mtr2p constitute a novel mRNA export complex which can bind to RNA via Mex67p and which interacts with nuclear pores via Mtr2p. ..
- Häcker S, Krebber H. Differential export requirements for shuttling serine/arginine-type mRNA-binding proteins. J Biol Chem. 2004;279:5049-52 pubmed..These findings suggest that Hrb1 and Gbp2, but not Npl3, might be loaded onto the growing mRNA via the THO complex components Mtf1 and Hrp1. ..
- Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E. The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding. Mol Cell. 2005;18:355-67 pubmed..Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha. ..
- Frey S, Richter R, Gorlich D. FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties. Science. 2006;314:815-7 pubmed..Furthermore, we obtained evidence that such hydrogel formation is required for viability in yeast. ..
- Yao W, Roser D, Köhler A, Bradatsch B, Bassler J, Hurt E. Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2. Mol Cell. 2007;26:51-62 pubmed..Thus, the general mRNA exporter Mex67-Mtr2 contains a distinct electrostatic interaction surface for transporting 60S preribosomal cargo. ..
- von Knethen A, Tzieply N, Jennewein C, Brüne B. Casein-kinase-II-dependent phosphorylation of PPARgamma provokes CRM1-mediated shuttling of PPARgamma from the nucleus to the cytosol. J Cell Sci. 2010;123:192-201 pubmed publisher..We conclude that CK-II-dependent PPARgamma phosphorylation at Ser16 and Ser21 is necessary for CRM1/Ran/RanBP3-mediated nucleocytoplasmic translocation of PPARgamma. ..
- Gailus S, Suormala T, Malerczyk Aktas A, Toliat M, Wittkampf T, Stucki M, et al. A novel mutation in LMBRD1 causes the cblF defect of vitamin B(12) metabolism in a Turkish patient. J Inherit Metab Dis. 2010;33:17-24 pubmed publisher..This case adds to the spectrum of clinical presentations and mutations of this rare disorder of lysosomal transport. ..
- Hetzer M, Meyer H, Walther T, Bilbao Cortes D, Warren G, Mattaj I. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Nat Cell Biol. 2001;3:1086-91 pubmed..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation. ..
- Englmeier L, Fornerod M, Bischoff F, Petosa C, Mattaj I, Kutay U. RanBP3 influences interactions between CRM1 and its nuclear protein export substrates. EMBO Rep. 2001;2:926-32 pubmed..These data indicate that RanBP3 functions by a novel mechanism as a cofactor in recognition and export of certain CRM1 substrates. In vitro, RanBP3 binding to CRM1 affects the relative affinity of CRM1 for different substrates. ..
- Batisse J, Batisse C, Budd A, Bottcher B, Hurt E. Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem. 2009;284:34911-7 pubmed publisher..Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure. ..
- Arts G, Kuersten S, Romby P, Ehresmann B, Mattaj I. The role of exportin-t in selective nuclear export of mature tRNAs. EMBO J. 1998;17:7430-41 pubmed..These results suggest that at least two mechanisms are involved in discrimination of pre-tRNAs and mature tRNAs prior to nuclear export. ..
- Hurt E, Strasser K, Segref A, Bailer S, Schlaich N, Presutti C, et al. Mex67p mediates nuclear export of a variety of RNA polymerase II transcripts. J Biol Chem. 2000;275:8361-8 pubmed..Thus, Mex67p is an mRNA export factor for a broad range of polymerase II transcripts. ..
- Young J, Obermann W, Hartl F. Specific binding of tetratricopeptide repeat proteins to the C-terminal 12-kDa domain of hsp90. J Biol Chem. 1998;273:18007-10 pubmed..In reticulocyte lysate, the C90 fusion protein recognized the TPR proteins p60, FKBP52, and Cyp40. Thus, our results identify the C90 domain as the specific binding site for a set of hsp90 cofactors having TPR domains. ..
- Senger B, Simos G, Bischoff F, Podtelejnikov A, Mann M, Hurt E. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p. EMBO J. 1998;17:2196-207 pubmed..This suggests that Npl3p follows a distinct nuclear import pathway and that intranuclear release from its specific import receptor Mtr10p requires the cooperative action of both Ran-GTP and newly synthesized mRNA. ..
- Cook A, Bono F, Jinek M, Conti E. Structural biology of nucleocytoplasmic transport. Annu Rev Biochem. 2007;76:647-71 pubmed
- Hoppe T, Matuschewski K, Rape M, Schlenker S, Ulrich H, Jentsch S. Activation of a membrane-bound transcription factor by regulated ubiquitin/proteasome-dependent processing. Cell. 2000;102:577-86 pubmed..Intriguingly, proteasome-dependent processing of SPT23 is regulated by fatty acid pools, suggesting that the precursor itself or interacting partners are sensors of membrane composition or fluidity. ..
- Gross T, Siepmann A, Sturm D, Windgassen M, Scarcelli J, Seedorf M, et al. The DEAD-box RNA helicase Dbp5 functions in translation termination. Science. 2007;315:646-9 pubmed..Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events. ..
- Mourão A, Varrot A, Mackereth C, Cusack S, Sattler M. Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX. RNA. 2010;16:1205-16 pubmed publisher..Our data suggest that the PHAX-RBD mediates auxiliary RNA contacts with the snRNA and snoRNA substrates that are required for transport and/or substrate release. ..
- Strasser K, Hurt E. Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. Nature. 2001;413:648-52 pubmed..Sub2p may then be displaced from Yra1p by the binding of Mex67p/Mtr2p, which participates in the export of mRNA through the nuclear pores. ..
- Braun S, Matuschewski K, Rape M, Thoms S, Jentsch S. Role of the ubiquitin-selective CDC48(UFD1/NPL4 )chaperone (segregase) in ERAD of OLE1 and other substrates. EMBO J. 2002;21:615-21 pubmed..We propose that CDC48(UFD1/NPL4) functions as a segregase that liberates ubiquitylated proteins from non-modified partners. ..
- Kuersten S, Arts G, Walther T, Englmeier L, Mattaj I. Steady-state nuclear localization of exportin-t involves RanGTP binding and two distinct nuclear pore complex interaction domains. Mol Cell Biol. 2002;22:5708-20 pubmed..We propose that these interactions increase the concentration of tRNA export complexes and of empty Xpo-t in the vicinity of NPCs and thus increase the efficiency of the Xpo-t transport cycle. ..
- Guria A, Tran D, Ramachandran S, Koch A, El Bounkari O, Dutta P, et al. Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts. RNA. 2011;17:1048-56 pubmed publisher..Furthermore, Hsp70 mRNA was exported in the absence of THOC5 at 37°C, but not under heat shock condition (42°C), suggesting that THOC5 may be required for mRNA export under stress and/or upon signaling-induced conditions. ..
- Fribourg S, Conti E. Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15. EMBO Rep. 2003;4:699-703 pubmed..In addition, Mtr2 presents a long internal loop, which contains residues that affect the export of the large ribosomal subunit. ..
- Yamada M, Mattaj I, Yoneda Y. An ATP-dependent activity that releases RanGDP from NTF2. J Biol Chem. 2004;279:36228-34 pubmed..A protein phosphatase inhibitor mixture stimulates the RanGDF activity, suggesting the activity is regulated by phosphorylation. We propose that the ATP-dependent NTF2 releasing factor may have a role in the RanGDP/GTP cycle. ..
- Bauer A, Kölling R. Characterization of the SAC3 gene of Saccharomyces cerevisiae. Yeast. 1996;12:965-75 pubmed..In the act1-1 mutant, however, upon shift to non-permissive temperature, newly synthesized cell wall material, instead of being directed towards the bud, was deposited at discrete spots in the mother cell. ..
- Bailer S, Siniossoglou S, Podtelejnikov A, Hellwig A, Mann M, Hurt E. Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p. EMBO J. 1998;17:1107-19 pubmed..Thus, the in vivo function of Gle2p is strictly coupled to the short GLEBS within Nup116p which links this putative mRNA transport factor to the nuclear pores. ..
- Segref A, Sharma K, Doye V, Hellwig A, Huber J, Luhrmann R, et al. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J. 1997;16:3256-71 pubmed..Thus, Mex67p is likely to participate directly in the export of mRNA from the nucleus to the cytoplasm. ..
- Kunzler M, Hurt E. Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett. 1998;433:185-90 pubmed..These findings suggest that Cse1p is the exportin of importin alpha in yeast. ..
- Rutsch F, Gailus S, Miousse I, Suormala T, Sagné C, Toliat M, et al. Identification of a putative lysosomal cobalamin exporter altered in the cblF defect of vitamin B12 metabolism. Nat Genet. 2009;41:234-9 pubmed publisher..This work identifies LMBRD1 as the gene underlying the cblF defect of cobalamin metabolism and suggests that LMBD1 is a lysosomal membrane exporter for cobalamin. ..
- Grosshans H, Hurt E, Simos G. An aminoacylation-dependent nuclear tRNA export pathway in yeast. Genes Dev. 2000;14:830-40 pubmed..Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries. ..
- Strasser K, Bassler J, Hurt E. Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol. 2000;150:695-706 pubmed
- Lemm I, Girard C, Kuhn A, Watkins N, Schneider M, Bordonné R, et al. Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies. Mol Biol Cell. 2006;17:3221-31 pubmed..Altogether, our data suggest that CBs have a modular structure with distinct domains for spliceosomal U snRNPs and snoRNPs. ..
- Kubitscheck U, GRUNWALD D, Hoekstra A, Rohleder D, Kues T, Siebrasse J, et al. Nuclear transport of single molecules: dwell times at the nuclear pore complex. J Cell Biol. 2005;168:233-43 pubmed..Together with the known transport rates, our data suggest that nucleocytoplasmic transport occurs via multiple parallel pathways within single NPCs. ..
- Fischer T, Strasser K, Rácz A, Rodriguez Navarro S, Oppizzi M, Ihrig P, et al. The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. EMBO J. 2002;21:5843-52 pubmed..Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC. ..
- Windgassen M, Krebber H. Identification of Gbp2 as a novel poly(A)+ RNA-binding protein involved in the cytoplasmic delivery of messenger RNAs in yeast. EMBO Rep. 2003;4:278-83 pubmed..Together, our findings clearly show an involvement of Gbp2 in mRNA transport. In addition, as a non-essential protein, Gbp2 also has the interesting potential to be spatially or temporally regulated. ..