Experts and Doctors on mutagenesis in Japan

Summary

Locale: Japan
Topic: mutagenesis

Top Publications

  1. Sato Y, Yamamoto Y, Kizaki H. Cloning and sequence analysis of the gbpC gene encoding a novel glucan-binding protein of Streptococcus mutans. Infect Immun. 1997;65:668-75 pubmed
    ..Extract from an E. coli clone of the gene exhibited glucan-binding activity. Therefore, the gene encoded a novel glucan-binding protein. ..
  2. Michikawa M, Ichinose H, Momma M, Biely P, Jongkees S, Yoshida M, et al. Structural and biochemical characterization of glycoside hydrolase family 79 β-glucuronidase from Acidobacterium capsulatum. J Biol Chem. 2012;287:14069-77 pubmed publisher
  3. Yamanaka K, Ogura T, Niki H, Hiraga S. Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli. J Bacteriol. 1992;174:7517-26 pubmed
    ..The smbA mutants are sensitive to a detergent, sodium dodecyl sulfate, and they show a novel morphological phenotype under nonpermissive conditions, suggesting a defect in specific membrane sites. ..
  4. Koike S, Ise I, Nomoto A. Functional domains of the poliovirus receptor. Proc Natl Acad Sci U S A. 1991;88:4104-8 pubmed
    ..Thus, the cytoplasmic domain of the molecule appears not to play a role in the penetration of poliovirus. ..
  5. Tokino T, Urano T, Furuhata T, Matsushima M, Miyatsu T, Sasaki S, et al. Characterization of the human p57KIP2 gene: alternative splicing, insertion/deletion polymorphisms in VNTR sequences in the coding region, and mutational analysis. Hum Genet. 1996;97:625-31 pubmed
    ..5'-CCGGCC-3', are tandemly repeated. ..
  6. Kuno K, Matsushima K. ADAMTS-1 protein anchors at the extracellular matrix through the thrombospondin type I motifs and its spacing region. J Biol Chem. 1998;273:13912-7 pubmed
    ..These results suggest that the ADAMTS-1 is a unique ADAM family protein that anchors at the ECM. ..
  7. Komatsuzawa H, Ohta K, Sugai M, Fujiwara T, Glanzmann P, Berger BächiB -, et al. Tn551-mediated insertional inactivation of the fmtB gene encoding a cell wall-associated protein abolishes methicillin resistance in Staphylococcus aureus. J Antimicrob Chemother. 2000;45:421-31 pubmed
    ..Inactivation of the fmtB gene seems therefore to have an indirect effect on methicillin resistance which can be relieved by increasing the production of the cell wall precursor glucosamine-1-phosphate. ..
  8. Ishida M, Oshima T, Yutani K. Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus. FEMS Microbiol Lett. 2002;216:179-83 pubmed
    ..The expression of the trpA gene was also improved ( approximately 1.5-fold). This approach may be useful for overexpressing various kinds of AT-rich genes. ..
  9. Liang S, Wei F, Wu Y, Tanabe K, Abe T, Oda Y, et al. Major Cdk5-dependent phosphorylation sites of amphiphysin 1 are implicated in the regulation of the membrane binding and endocytosis. J Neurochem. 2007;102:1466-1476 pubmed publisher
    ..Phosphorylation at these sites alters binding of amph 1 to lipid membranes, and may be an important regulatory aspect in the regulation of synaptic vesicle endocytosis. ..

More Information

Publications164 found, 100 shown here

  1. Yee L, Matsuoka S, Yano K, Sadaie Y, Asai K. Inhibitory effect of prophage SP? fragments on phage SP10 ribonucleotide reductase function and its multiplication in Bacillus subtilis. Genes Genet Syst. 2011;86:7-18 pubmed
    ..It is thus likely that the transcript from the bnrdEF region of SP? inhibits ribonucleotide reductase function of SP10, resulting in arrest of DNA synthesis during phage SP10 development. ..
  2. Sato S, Kitamoto D, Habe H. Chemical mutagenesis of Gluconobacter frateurii to construct methanol-resistant mutants showing glyceric acid production from methanol-containing glycerol. J Biosci Bioeng. 2014;117:197-199 pubmed publisher
    ..The obtained mutant Gf398 produced 6.3 g/L GA in 5% (v/v) methanol-containing 17% (w/v) glycerol medium, in which the wild-type strain neither grew nor produced GA. ..
  3. Nakatsuji Y, Hidaka K, Tsujino S, Yamamoto Y, Mukai T, Yanagihara T, et al. A single MEF-2 site is a major positive regulatory element required for transcription of the muscle-specific subunit of the human phosphoglycerate mutase gene in skeletal and cardiac muscle cells. Mol Cell Biol. 1992;12:4384-90 pubmed
    ..These observations define the PGAM-M enhancer as the only cardiac- and skeletal-muscle-specific enhancer characterized thus far that is mainly activated through MEF-2. ..
  4. Hisamoto N, Frederick D, Sugimoto K, Tatchell K, Matsumoto K. The EGP1 gene may be a positive regulator of protein phosphatase type 1 in the growth control of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3767-76 pubmed
    ..Egp1 and PP1 directly interact, as assayed by coimmunoprecipitation. These results suggest that Egp1 functions as a positive modulator of PP1 in the growth control of S. cerevisiae. ..
  5. Muto A, Watanabe S, Miyajima A, Yokota T, Arai K. The beta subunit of human granulocyte-macrophage colony-stimulating factor receptor forms a homodimer and is activated via association with the alpha subunit. J Exp Med. 1996;183:1911-6 pubmed
    ..These results indicate that the beta homodimer, which alone is insufficient for signaling, forms the functional hGMR with the alpha subunit in response to hGM-CSF. ..
  6. Inoue J, Iwaoka T, Tokunaga H, Takamune K, Naomi S, Araki M, et al. A family with Liddle's syndrome caused by a new missense mutation in the beta subunit of the epithelial sodium channel. J Clin Endocrinol Metab. 1998;83:2210-3 pubmed
    ..001). These findings provide further clinical evidence for the hypothesis that a conserved PY motif may be critically important for the regulation of the epithelial sodium channel. ..
  7. Oka M, Miyamoto K, Okada K, Ueda J. Auxin polar transport and flower formation in Arabidopsis thaliana transformed with indoleacetamide hydrolase (iaaH) gene. Plant Cell Physiol. 1999;40:231-7 pubmed
    ..These results suggest that the construction of the system of auxin polar transport and its normal activities are essential for the differentiation and the formation of floral meristem in the early growth stage of Arabidopsis thaliana. ..
  8. Yoshida T, Kokura K, Makino Y, Ossipow V, Tamura T. Heterogeneous nuclear RNA-ribonucleoprotein F binds to DNA via an oligo(dG)-motif and is associated with RNA polymerase II. Genes Cells. 1999;4:707-19 pubmed
    ..The DNA-binding ability of hnRNP-F might facilitate the entry of pre-mRNA modification/splicing factors at a promoter. ..
  9. Matsushima Y, Matsumura K, Ishii S, Inagaki H, Suzuki T, Matsuda Y, et al. Functional domains of chicken mitochondrial transcription factor A for the maintenance of mitochondrial DNA copy number in lymphoma cell line DT40. J Biol Chem. 2003;278:31149-58 pubmed
    ..A series of deletion mutant at the tail region suggested stimulatory and suppressive sequences in this region for the maintenance of mtDNA level. ..
  10. Fujimoto Z, Ichinose H, Maehara T, Honda M, Kitaoka M, Kaneko S. Crystal structure of an Exo-1,5-{alpha}-L-arabinofuranosidase from Streptomyces avermitilis provides insights into the mechanism of substrate discrimination between exo- and endo-type enzymes in glycoside hydrolase family 43. J Biol Chem. 2010;285:34134-43 pubmed publisher
  11. Miyazaki K, Takenouchi M. Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques. 2002;33:1033-4, 1036-8 pubmed
    ..The present method, which we designated MEGAWHOP (megaprimer PCR of whole plasmid), is thus ideal for creating random mutagenesis megalibraries. ..
  12. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000;102:849-62 pubmed
    ..Our results suggest that p53AIP1 is likely to play an important role in mediating p53-dependent apoptosis, and phosphorylation of Ser-46 regulates the transcriptional activation of this apoptosis-inducing gene. ..
  13. Kanie S, Horibata K, Kawano M, Isogawa A, Sakai A, Matsuo N, et al. Roles of RecA protein in spontaneous mutagenesis in Escherichia coli. Genes Genet Syst. 2007;82:99-108 pubmed
    ..Thus, it seems likely that the single-base frameshifts are derived from slippage errors that are not directly caused by DNA lesions but made indirectly during some kind of error-prone DNA synthesis in the recA mutant cells. ..
  14. Murakami H, Horihata M, Andojo S, Yoneda Kato N, Kato J. Isolation and characterization of cytoplasmic cyclin D1 mutants. FEBS Lett. 2009;583:1575-80 pubmed publisher
    ..We propose that interaction between cyclin D1 and Cdk4 triggers the mechanism controlling the nuclear transportation of this kinase complex. ..
  15. Kamata A, Sakagami H, Tokumitsu H, Owada Y, Fukunaga K, Kondo H. Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth. Neurosci Res. 2007;57:86-97 pubmed
  16. Suzuki Fujimoto T, Fukuma M, Yano K, Sakurai H, Vonika A, Johnston S, et al. Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: interaction between Gal3p and Gal80p. Mol Cell Biol. 1996;16:2504-8 pubmed
    ..Reexamination of the DNA sequence of GAL3 revealed several mistakes, including an extension at the 3' end of another predicted 97 amino acids. ..
  17. Yamamoto J, Fukui T, Suzuki K, Tanaka S, Yatsunami K, Ichikawa A. Expression and characterization of recombinant mouse mastocytoma histidine decarboxylase. Biochim Biophys Acta. 1993;1216:431-40 pubmed
    ..These results strongly suggest that the 53 kDa subunit peptide of native mastocytoma HDC is derived from the unidentified inactive 74 kDa HDC peptide, probably by post-translational processing of HDC in its C-terminal region. ..
  18. Arimoto Kobayashi S, Anma N, Yoshinaga Y, Douki T, Cadet J, Hayatsu H. Oxidative damage and induced mutations in m13mp2 phage DNA exposed to N-nitrosopyrrolidine with UVA radiation. Mutagenesis. 2000;15:473-7 pubmed
    ..With these hosts, different hot spots were observed and a new GC-->TA hot spot was produced. The formation of 8-oxodGuo in DNA, which is known to induce GC-->TA transversion, may contribute to mutagenesis by NPYR plus UVA. ..
  19. Takahashi S, Kasai K, Hatsuzawa K, Kitamura N, Misumi Y, Ikehara Y, et al. A mutation of furin causes the lack of precursor-processing activity in human colon carcinoma LoVo cells. Biochem Biophys Res Commun. 1993;195:1019-26 pubmed
    ..Based on these data, we conclude that furin is the endoprotease that is involved in the precursor cleavage at RXK/RR sites within the constitutive secretory pathway. ..
  20. Supino Rosin L, Yoshimura A, Altaratz H, Neumann D. A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation. Eur J Biochem. 1999;263:410-9 pubmed
    ..A truncated EGF-R/EPO-R chimera containing only 50 amino acids of the EPO-R membrane-proximal cytosolic part was also rapidly degraded suggesting that these 50 amino acids are involved in receptor degradation. ..
  21. Kuwahara M, Asai T, Terada Y, Sasaki S. The C-terminal tail of aquaporin-2 determines apical trafficking. Kidney Int. 2005;68:1999-2009 pubmed
    ..The putative PDZ-interacting motif (G-T-K-A, residues 268-271) plays a key role in apical membrane expression. In addition, cAMP-dependent phosphorylation was found to be critical for apical targeting. ..
  22. Meno C, Gritsman K, Ohishi S, Ohfuji Y, Heckscher E, Mochida K, et al. Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. Mol Cell. 1999;4:287-98 pubmed
    ..Expression of antivin is dependent on Nodal signaling, revealing a feedback loop wherein Nodal signals induce their antagonists Lefty2 and Antivin to restrict Nodal signaling during gastrulation. ..
  23. Okuhata R, Otsuka Y, Tsuchiya T, Kanzawa N. Mutagenesis of apyrase conserved region 1 alters the nucleotide substrate specificity. Plant Signal Behav. 2013;8:e24131 pubmed publisher
    ..19). Binding affinity for ADP of A75S-MpAPY2 mutant was increased to a level higher than that of the wild type MpAPY2. Thus, the residue at position X in the DXG motif plays an important role in determining nucleotide preference. ..
  24. Takeda H, Rust A, Ward J, Yew C, Jenkins N, Copeland N. Sleeping Beauty transposon mutagenesis identifies genes that cooperate with mutant Smad4 in gastric cancer development. Proc Natl Acad Sci U S A. 2016;113:E2057-65 pubmed publisher
    ..SB mutagenesis has, thus, helped to catalog the cooperative molecular mechanisms driving SMAD4-induced GC growth and discover genes with potential clinical importance in human GC. ..
  25. Nagano H, Okuno T, Mise K, Furusawa I. Deletion of the C-terminal 33 amino acids of cucumber mosaic virus movement protein enables a chimeric brome mosaic virus to move from cell to cell. J Virol. 1997;71:2270-6 pubmed
  26. Kyuma M, Ura N, Torii T, Takeuchi H, Takizawa H, Kitamura K, et al. A family with liddle's syndrome caused by a mutation in the beta subunit of the epithelial sodium channel. Clin Exp Hypertens. 2001;23:471-8 pubmed
    ..The mutation of the ENaC of this family was beta R564X. Since such case seem to be rare in the literature, detailed data are shown in this report. ..
  27. Sasaki M, Kurusu Y. Analysis of spontaneous base substitutions generated in mutator strains of Bacillus subtilis. FEMS Microbiol Lett. 2004;234:37-42 pubmed
    ..In contrary, transversion was high on mutY and double mutant (mutM mutY). Finally, the opuBC and yitG genes were identified from the B. subtilis chromosome as mutator genes that prevented the transition base substitutions. ..
  28. Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, et al. A high-speed congenic strategy using first-wave male germ cells. PLoS ONE. 2009;4:e4943 pubmed publisher
    ..It should provide the fastest protocol for precise definition of the phenotypic effects of genes of interest on desired genetic backgrounds. ..
  29. Gondo Y. Trends in large-scale mouse mutagenesis: from genetics to functional genomics. Nat Rev Genet. 2008;9:803-10 pubmed publisher
    ..This article traces the origins and rationale of these large-scale mouse mutagenesis programmes. ..
  30. Wada Y, Furuse T, Yamada I, Masuya H, Kushida T, Shibukawa Y, et al. ENU mutagenesis screening for dominant behavioral mutations based on normal control data obtained in home-cage activity, open-field, and passive avoidance tests. Exp Anim. 2010;59:495-510 pubmed
    ..Genetic and detailed behavioral analysis of these ENU-induced mutants will provide novel insights into the molecular mechanisms underlying behavior. ..
  31. Yoshida T, Toda T, Yanagida M. A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning. J Cell Sci. 1994;107 ( Pt 7):1725-35 pubmed
    ..Thus the ppb1+ gene appears to be involved in cytokinesis, mating, transport, nuclear and spindle pole body positioning, and cell shape. ..
  32. Shirasaka N, Nishi K, Shimizu S. Biosynthesis of furan fatty acids (F-acids) by a marine bacterium, Shewanella putrefaciens. Biochim Biophys Acta. 1997;1346:253-60 pubmed
    ..Finally, the amount of F18 began to increase after 20 h and reached a plateau at 36 h. These results suggest that 11-me-18:1 and 11-me-18:2 are precursors of F18. ..
  33. Terada Y, Nakashima O, Inoshita S, Kuwahara M, Sasaki S, Marumo F. TGF-beta-activating kinase-1 inhibits cell cycle and expression of cyclin D1 and A in LLC-PK1 cells. Kidney Int. 1999;56:1378-90 pubmed
    ..Cyclin D1 and A promoter activity and cell cycle progression in renal tubular cells are negatively regulated by the TAK1-MKK6-p38K pathway and positively regulated by the MKK1-p42/44MAPK pathway. ..
  34. Kitamura K, Maekawa H, Shimoda C. Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase. Mol Biol Cell. 1998;9:1065-80 pubmed
    ..In these cells, mitosis was inhibited and an extra round of DNA replication occurred. Ste9 regulates G1 progression possibly by controlling the amount of the mitotic cyclin in the G1-phase. ..
  35. Yamada Okabe T, Mio T, Matsui M, Kashima Y, Arisawa M, Yamada Okabe H. Isolation and characterization of the Candida albicans gene for mRNA 5'-triphosphatase: association of mRNA 5'-triphosphatase and mRNA 5'-guanylyltransferase activities is essential for the function of mRNA 5'-capping enzyme in vivo. FEBS Lett. 1998;435:49-54 pubmed
  36. Doi T, Higashino K, Kurihara Y, Wada Y, Miyazaki T, Nakamura H, et al. Charged collagen structure mediates the recognition of negatively charged macromolecules by macrophage scavenger receptors. J Biol Chem. 1993;268:2126-33 pubmed
    ..Based on computational modeling of this domain, we propose that a "charged collagen" structure containing a lysine cluster forms a positively charged groove which specifically interacts with negatively charged ligands. ..
  37. Ueda T, Yoshizumi T, Anai T, Matsui M, Uchimiya H, Nakano A. AtGDI2, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor. Gene. 1998;206:137-43 pubmed
    ..Genomic DNA hybridization using specific probes reveals the presence of one more GDI gene in Arabidopsis. This may imply differentiated roles of GDI in higher plants. ..
  38. Chida K, Nagamori S, Kuroki T. Nuclear translocation of Fos is stimulated by interaction with Jun through the leucine zipper. Cell Mol Life Sci. 1999;55:297-302 pubmed
  39. Tsukahara T, Agawa H, Matsumoto S, Matsuda M, Ueno S, Yamashita Y, et al. Murine leukemia virus vector integration favors promoter regions and regional hot spots in a human T-cell line. Biochem Biophys Res Commun. 2006;345:1099-107 pubmed
    ..These studies help define the profile of MLV integration in T-cells and the risks associated with MLV-based gene therapy. ..
  40. Suemori A, Iwakura M. A systematic and comprehensive combinatorial approach to simultaneously improve the activity, reaction specificity, and thermal stability of p-hydroxybenzoate hydroxylase. J Biol Chem. 2007;282:19969-78 pubmed
  41. Akiyama Y, Ogura T, Ito K. Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter. J Biol Chem. 1994;269:5218-24 pubmed
    ..These results suggest that FtsH participates in assembly of proteins into and through the membrane. It is needed for the cell to assure efficient stop-transfer of some transmembrane proteins. ..
  42. Tsuneizumi K, Nakayama T, Kamoshida Y, Kornberg T, Christian J, Tabata T. Daughters against dpp modulates dpp organizing activity in Drosophila wing development. Nature. 1997;389:627-31 pubmed
    ..We conclude that the pattern-organizing mechanism governed by Dpp involves a negative-feedback circuit in which Dpp induces expression of its own antagonist, Dad. This feedback loop appears to be conserved in vertebrate development. ..
  43. Sun G, Hirata A, Ohya Y, Anraku Y. Mutations in yeast calmodulin cause defects in spindle pole body functions and nuclear integrity. J Cell Biol. 1992;119:1625-39 pubmed
    ..The single SPB frequently localizes on the projections or the invaginations of the nuclear envelope. These observations suggest that CaM is required for the functions of SPB and spindle, and the integrity of nucleus. ..
  44. Ukita T, Ikeda H. Role of the recJ gene product in UV-induced illegitimate recombination at the hotspot. J Bacteriol. 1996;178:2362-7 pubmed
    ..We propose a model based on the 5'-3' exonuclease activity of RecJ to explain the involvement of this protein in illegitimate recombination at the hotspot. ..
  45. Shikazono N, Pearson C, O Neill P, Thacker J. The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA base damage. Nucleic Acids Res. 2006;34:3722-30 pubmed
    ..Recovered plasmids commonly gave rise to both wild-type and mutant progeny, suggesting that there is differential replication of the two DNA strands carrying specific forms of base damage. ..
  46. Umemori J, Takao K, Koshimizu H, Hattori S, Furuse T, Wakana S, et al. ENU-mutagenesis mice with a non-synonymous mutation in Grin1 exhibit abnormal anxiety-like behaviors, impaired fear memory, and decreased acoustic startle response. BMC Res Notes. 2013;6:203 pubmed publisher
    ..Although Grin1(Rgsc174)/Grin1+ mice only partially recapitulate symptoms of patients with ADHD, schizophrenia, and bipolar disorder, they may serve as a unique animal model of a certain subpopulation of patients with these disorders. ..
  47. Uchiumi F, Semba K, Yamanashi Y, Fujisawa J, Yoshida M, Inoue K, et al. Characterization of the promoter region of the src family gene lyn and its trans activation by human T-cell leukemia virus type I-encoded p40tax. Mol Cell Biol. 1992;12:3784-95 pubmed
  48. Yokokura H, Picciotto M, Nairn A, Hidaka H. The regulatory region of calcium/calmodulin-dependent protein kinase I contains closely associated autoinhibitory and calmodulin-binding domains. J Biol Chem. 1995;270:23851-9 pubmed
    ..2 microM) but was non-competitive with ATP. Thus, these results suggest that CaM kinase I is regulated through an intrasteric mechanism common to other members of the family of Ca2+/CaM-dependent protein kinases. ..
  49. Yasuda T, Morimatsu K, Kato R, Usukura J, Takahashi M, Ohmori H. Physical interactions between DinI and RecA nucleoprotein filament for the regulation of SOS mutagenesis. EMBO J. 2001;20:1192-202 pubmed
    ..DinI is thus suited to modulating UmuD processing so as to limit SOS mutagenesis. ..
  50. Muramoto T, Takeda S, Furuya Y, Urushihara H. Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum. Mech Dev. 2005;122:733-43 pubmed publisher
    ..Knockout mutants of tmcC were indistinguishable from the wild type cells with respect to the cAMP response, suggesting that this gene is relevant to other processes...
  51. Kitano K, Kim S, Hakoshima T. Structural basis for DNA strand separation by the unconventional winged-helix domain of RecQ helicase WRN. Structure. 2010;18:177-87 pubmed publisher
    ..Our results demonstrate a function of the winged-helix motif central to the helicase reaction, establishing the first structural paradigm concerning the DNA structure-specific activities of the RecQ helicases. ..
  52. Nakai M, Kato Y, Ikeda E, Toh e A, Endo T. Yge1p, a eukaryotic Grp-E homolog, is localized in the mitochondrial matrix and interacts with mitochondrial Hsp70. Biochem Biophys Res Commun. 1994;200:435-42 pubmed
    ..We propose that Yge1p functions in cooperation with Ssc1p in a similar manner as bacterial GrpE with DnaK. ..
  53. Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H. Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose. J Mol Biol. 1998;277:393-407 pubmed
    ..2 A2 among the five sugar residues. Three calcium ions are found, one of which is positioned near the substrate binding site as found in other alpha-amylases and could contribute to stabilization of the structure of the active site. ..
  54. Iida T, Kobayashi S. Delocalization of polar plasm components caused by grandchildless mutations, gs(1)N26 and gs(1)N441, in Drosophila melanogaster. Dev Growth Differ. 2000;42:53-60 pubmed
    ..This indicates that the segregation of polar plasm into pole cells is required to maintain polar plasm integrity. The mechanism regulating polar plasm integrity in embryos is discussed. ..
  55. Jałoszyński P, Ohashi E, Ohmori H, Nishimura S. Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene fragments by DNA polymerase kappa. Genes Cells. 2005;10:543-50 pubmed
    ..Interestingly, in direct mutagenesis systems, the preference for dAMP over dCMP was nearly the same on mouse and human templates. ..
  56. Tajima S, Itoh Y, Sugimoto T, Kato T, Park E. Increased riboflavin production from activated bleaching earth by a mutant strain of Ashbya gossypii. J Biosci Bioeng. 2009;108:325-9 pubmed publisher
    ..This novel mutant has the potential to be of use for industrial-scale riboflavin production from waste-activated bleaching earth (ABE), thereby transforming a useless material into a valuable bioproduct. ..
  57. Nakazawa T, Tatsuta Y, Fujita T, Nakahori K, Kamada T. Mutations in the Cc.rmt1 gene encoding a putative protein arginine methyltransferase alter developmental programs in the basidiomycete Coprinopsis cinerea. Curr Genet. 2010;56:361-7 pubmed publisher
    ..Cc.rmt1 is predicted to encode a putative protein arginine methyltransferase, some homologs of which have been shown to be involved in the regulation of gene expression in eukaryotes. ..
  58. Matsui I, Ishikawa K, Miyairi S, Fukui S, Honda K. An increase in the transglycosylation activity of Saccharomycopsis alpha-amylase altered by site-directed mutagenesis. Biochim Biophys Acta. 1991;1077:416-9 pubmed
    ..These results suggest that the tryptophan residue located at subsite 3 of the enzyme plays an important role not only to hold the substrate, but also to liberate the hydrolysis products from the substrate binding pocket. ..
  59. Adachi N, Ikeda H, Kikuchi A. Mutant isolation of mouse DNA topoisomerase II alpha in yeast. Nucleic Acids Res. 1994;22:4229-33 pubmed
    ..Therefore we have isolated the first conditional mutation in the mouse TOP2 alpha. ..
  60. Sugimoto K, Shimomura T, Hashimoto K, Araki H, Sugino A, Matsumoto K. Rfc5, a small subunit of replication factor C complex, couples DNA replication and mitosis in budding yeast. Proc Natl Acad Sci U S A. 1996;93:7048-52 pubmed
    ..Taken together, these results suggested that replication factor C has a direct role in sensing the state of DNA replication and transmitting the signal to the checkpoint machinery. ..
  61. Hamaguchi I, Yamaguchi N, Suda J, Iwama A, Hirao A, Hashiyama M, et al. Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice. Biochem Biophys Res Commun. 1996;224:172-9 pubmed
    ..CHK/HYL did not affect the activity of Src, Hck, and Fgr in cultured bone marrow cells, although CSK negatively regulates Src family kinases. These results suggest that CHK/HYL might not have the same function as CSK. ..
  62. Hashimoto H, Yoda K. Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus. Biochem Biophys Res Commun. 1997;241:682-6 pubmed
    ..These complexes with similar but partially different constituents may represent essential parts of glycosylation machinery in the yeast Golgi compartments. ..
  63. Yoshida H, Okada T, Haze K, Yanagi H, Yura T, Negishi M, et al. ATF6 activated by proteolysis binds in the presence of NF-Y (CBF) directly to the cis-acting element responsible for the mammalian unfolded protein response. Mol Cell Biol. 2000;20:6755-67 pubmed
    ..Based on these and other findings, we concluded that specific and direct interactions between ATF6 and ERSE are critical for transcriptional induction not only of ER chaperones but also of CHOP and XBP-1. ..
  64. Gondo Y, Fukumura R, Murata T, Makino S. ENU-based gene-driven mutagenesis in the mouse: a next-generation gene-targeting system. Exp Anim. 2010;59:537-48 pubmed
    ..In the near future, next-generation re-sequencing systems should drastically enhance the utility of the ENU-based gene-driven mutagenesis not only for the mouse but also for other species. ..
  65. Kuroda A, Kumano T, Taguchi K, Nikata T, Kato J, Ohtake H. Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa. J Bacteriol. 1995;177:7019-25 pubmed
    ..In vivo methyl labeling experiments with L-[methyl-3H]methionine showed that this knockout mutant lacked an MCP with a molecular weight of approximately 68,000. ..
  66. Watanabe Y, Takaesu G, Hagiwara M, Irie K, Matsumoto K. Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway. Mol Cell Biol. 1997;17:2615-23 pubmed
  67. Hamano Takaku F, Iwama T, Saito Yano S, Takaku K, Monden Y, Kitabatake M, et al. A mutant Escherichia coli tyrosyl-tRNA synthetase utilizes the unnatural amino acid azatyrosine more efficiently than tyrosine. J Biol Chem. 2000;275:40324-8 pubmed
    ..These results suggest that utilization of F130S TyrRS for in vivo protein biosynthesis may lead to efficient production of azatyrosine-containing alloproteins. ..
  68. Kawaguchi M, Imaizumi Anraku H, Koiwa H, Niwa S, Ikuta A, Syono K, et al. Root, root hair, and symbiotic mutants of the model legume Lotus japonicus. Mol Plant Microbe Interact. 2002;15:17-26 pubmed
    ..Mutants affecting the developmental process of nodule organogenesis were placed in three phenotypic categories: Nod- (Ljsym70 to Ljsym73), Hist- (alb1-1, alb1-2, and Ljsym79), and Fix- (fen1, Ljsym75, and Ljsym81). ..
  69. Inaoka T, Takahashi K, Yada H, Yoshida M, Ochi K. RNA polymerase mutation activates the production of a dormant antibiotic 3,3'-neotrehalosadiamine via an autoinduction mechanism in Bacillus subtilis. J Biol Chem. 2004;279:3885-92 pubmed
    ..Therefore, we propose that unlike the wild-type RNAP, the mutant RNAP efficiently recognized the sigma(A)-dependent promoters, resulting in the dramatic activation of the NTD biosynthesis pathway by an autoinduction mechanism. ..
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